Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- Selected Works (14)
- Brigham Young University (12)
- University of Nebraska - Lincoln (9)
- California Polytechnic State University, San Luis Obispo (8)
- University of Massachusetts Amherst (6)
-
- Western University (6)
- Old Dominion University (5)
- Technological University Dublin (5)
- Chapman University (4)
- Munster Technological University (4)
- San Jose State University (4)
- University of Missouri, St. Louis (4)
- Wayne State University (4)
- Central Washington University (3)
- Montclair State University (3)
- SelectedWorks (3)
- University of the Pacific (3)
- Clemson University (2)
- Dominican University of California (2)
- Marshall University (2)
- Portland State University (2)
- Syracuse University (2)
- University of Montana (2)
- University of South Carolina (2)
- Western Washington University (2)
- Aga Khan University (1)
- Cedarville University (1)
- City University of New York (CUNY) (1)
- Cleveland State University (1)
- Colby College (1)
- Keyword
-
- Models, Molecular (5)
- Biochemistry (4)
- Escherichia coli (4)
- Models (4)
- Molecular (4)
-
- Selected Peer-reviewed Publications (4)
- Biological Sciences (3)
- Protein Binding (3)
- Protein Structure (3)
- Protein Subunits (3)
- Amino acid (2)
- Aminoacyl-tRNA synthetase (2)
- Carrier Proteins (2)
- Catalytic Domain (2)
- Cloning (2)
- DNA (2)
- Dimerization (2)
- Escherichia coli Proteins (2)
- Gene expression (2)
- Hydrolysis (2)
- Maltose-Binding Proteins (2)
- Mass spectrometry (2)
- Microbiology (2)
- Molecular Sequence Data (2)
- Peroxisomes (2)
- Protein Structure, Tertiary (2)
- RA (2)
- Rotation (2)
- Saccharomyces cerevisiae (2)
- Streptavidin (2)
- Publication
-
- Department of Biochemistry: Faculty Publications (9)
- Chemistry and Biochemistry (8)
- Theses and Dissertations (8)
- Biochemistry Publications (6)
- Faculty Publications (6)
-
- Articles (5)
- Chemistry & Biochemistry Faculty Works (5)
- Biochemistry and Molecular Biology Faculty Publications (4)
- All Dugoni School of Dentistry Faculty Articles (3)
- All Faculty Scholarship for the College of the Sciences (3)
- Biology, Chemistry, and Environmental Sciences Faculty Articles and Research (3)
- Electronic Theses and Dissertations (3)
- Elizabeth Vierling (3)
- Nigam Rath (3)
- Theses (3)
- Theses, Dissertations and Culminating Projects (3)
- All Dissertations (2)
- Biochemistry and Microbiology (2)
- Chemistry Faculty Publications and Presentations (2)
- Chemistry and Biochemistry Faculty Publications (2)
- Danny Schnell (2)
- Daron E Janzen, Ph.D. (2)
- Daryl K. Eggers (2)
- Faculty Publications, Chemistry (2)
- Honors Capstone Projects - All (2)
- Natural Sciences and Mathematics | Faculty Scholarship (2)
- Yan Lu (2)
- Bioelectrics Publications (1)
- Biological Sciences Faculty Publications (1)
- Chemistry (1)
- Publication Type
- File Type
Articles 1 - 30 of 141
Full-Text Articles in Life Sciences
A Simple Spectrophotometric Streptavidin-Biotin Binding Assay Utilizing Biotin-4-Fluorescein., Mark Waner, David Mascotti
A Simple Spectrophotometric Streptavidin-Biotin Binding Assay Utilizing Biotin-4-Fluorescein., Mark Waner, David Mascotti
Mark J. Waner
A new assay for biotin binding capacity of Streptavidin (SA) is presented in this work. The assay is based on the large decrease in the extinction coefficient at 493 nm that accompanies binding of biotin-4-fluorescein (B4F) to SA. This decrease is attributed to formation of a charge transfer complex between the B4F-donor and one or more SA residues. We show that one may observe the stoichiometric binding via monitoring the absorbance at 493 nm using either SA or B4F as the titrant. The sensitivity of the assay is at the lower end of similar fluorimetric and photometric assays. Though the …
Laser-Induced Photon-Branched Chain Reaction In A Chemically-Active Gas-Dispersed Medium, Thomas George, Renat Letfullin, Galen Duree
Laser-Induced Photon-Branched Chain Reaction In A Chemically-Active Gas-Dispersed Medium, Thomas George, Renat Letfullin, Galen Duree
Chemistry & Biochemistry Faculty Works
A promising avenue in the development of high-energy pulsed chemical HF/DF lasers and amplifiers is the utilization of a photon-branched chain reaction initiated in a two-phase active medium, that is, a medium containing a laser working gas and ultradispersed passivated metal particles. These particles are evaporated under the action of IR laser radiation which results in the appearance of free atoms, their diffusion into the gas, and the development of a photon-branching chain process, which involves photons as both reactants and products. The key obstacle here is the formation of a relatively large volume (in excess of 10^3 cm^3) of …
Laser-Induced Photon-Branched Chain Reaction In A Chemically-Active Gas-Dispersed Medium, Thomas F. George, Renat R. Letfullin, Galen C. Duree
Laser-Induced Photon-Branched Chain Reaction In A Chemically-Active Gas-Dispersed Medium, Thomas F. George, Renat R. Letfullin, Galen C. Duree
Thomas George
Structure And Dynamics Of Metalloproteins In Live Cells, Jeremy D. Cook, James E. Penner-Hahn, Timothy L. Stemmler
Structure And Dynamics Of Metalloproteins In Live Cells, Jeremy D. Cook, James E. Penner-Hahn, Timothy L. Stemmler
Biochemistry and Molecular Biology Faculty Publications
X-ray absorption spectroscopy (XAS) has emerged as one of the premier tools for investigating the structure and dynamic properties of metals in cells and in metal containing biomolecules. Utilizing the high flux and broad energy range of X-rays supplied by synchrotron light sources, one can selectively excite core electronic transitions in each metal. Spectroscopic signals from these electronic transitions can be used to dissect the chemical architecture of metals in cells, in cellular components and in biomolecules at varying degrees of structural resolution. With the development of ever-brighter X-ray sources, X-ray methods have grown into applications that can be utilized …
The Effect Of Oxidant And The Non-Oxidant Alteration Of Cellular Thiol Concentration On The Formation Of Protein Mixed-Disulfides In Hek 293 Cells, Jasen Lee Gilge, Michael Fisher, Yuh-Cherng Chai
The Effect Of Oxidant And The Non-Oxidant Alteration Of Cellular Thiol Concentration On The Formation Of Protein Mixed-Disulfides In Hek 293 Cells, Jasen Lee Gilge, Michael Fisher, Yuh-Cherng Chai
Chemistry
Cellular molecules possess various mechanisms in responding to oxidant stress. In terms of protein responses, protein S-glutathionylation is a unique post-translational modification of protein reactive cysteines forming disulfides with glutathione molecules. This modification has been proposed to play roles in antioxidant, regulatory and signaling in cells under oxidant stress. Recently, the increased level of protein S-glutathionylation has been linked with the development of diseases. In this report, specific S-glutathionylated proteins were demonstrated in human embryonic kidney 293 cells treated with two different oxidative reagents: diamide and hydrogen peroxide. Diamide is a chemical oxidizing agent whereas hydrogen peroxide is a physiological …
Towards The Total Synthesis Of Thioviridamide: Thiyl Radical Approach To The Beta-Thioenamide Linkage Formation, Jung-Hoon Kang
Towards The Total Synthesis Of Thioviridamide: Thiyl Radical Approach To The Beta-Thioenamide Linkage Formation, Jung-Hoon Kang
Theses and Dissertations
We developed an approach to the β-thioenamide linkage contained in the S-(2-aminovinyl)cysteine (avCys) residue of thioviridamide.1,2 Kinetic and thermodynamic control of radical additions of thiols to ynamides were studied for the formation of β-thioenamide linkage. Thiyl radicals are electrophilic and ynamides are electron-rich alkynes. This complementary polarity of the radical and acceptor increases the likelihood of a successful radical addition reaction. Because little is known about these types of compounds (β-thioenamides), we were unsure what kinds of yields and stereoselectivities (cis vs. trans) to expect. The adduct stability is another issue to consider. Fortunately, under typical radical addition conditions, the …
Three-Dimensional Microfluidic Devices Fabricated In Layered Paper And Tape, Andres W. Martinez, Scott T. Phillips, George M. Whitesides
Three-Dimensional Microfluidic Devices Fabricated In Layered Paper And Tape, Andres W. Martinez, Scott T. Phillips, George M. Whitesides
Chemistry and Biochemistry
This article describes a method for fabricating 3D microfluidic devices by stacking layers of patterned paper and double-sided adhesive tape. Paper-based 3D microfluidic devices have capabilities in microfluidics that are difficult to achieve using conventional open-channel microsystems made from glass or polymers. In particular, 3D paper-based devices wick fluids and distribute microliter volumes of samples from single inlet points into arrays of detection zones (with numbers up to thousands). This capability makes it possible to carry out a range of new analytical protocols simply and inexpensively (all on a piece of paper) without external pumps. We demonstrate a prototype 3D …
Sinorhizobium Meliloti, A Bacterium Lacking The Autoinducer-2 (Ai-2) Synthase, Responds To Ai-2 Supplied By Other Bacteria, C. S. Pereira, J. R. Mcauley, M. E. Taga, K. B. Xavier, Stephen T. Miller
Sinorhizobium Meliloti, A Bacterium Lacking The Autoinducer-2 (Ai-2) Synthase, Responds To Ai-2 Supplied By Other Bacteria, C. S. Pereira, J. R. Mcauley, M. E. Taga, K. B. Xavier, Stephen T. Miller
Chemistry & Biochemistry Faculty Works
Many bacterial species respond to the quorum-sensing signal autoinducer-2 (AI-2) by regulating different niche-specific genes. Here, we show that Sinorhizobium meliloti, a plant symbiont lacking the gene for the AI-2 synthase, while not capable of producing AI-2 can nonetheless respond to AI-2 produced by other species. We demonstrate that S. meliloti has a periplasmic binding protein that binds AI-2. The crystal structure of this protein (here named SmlsrB) with its ligand reveals that it binds (2R,4S)-2-methyl-2,3,3,4-tetrahydroxytetrahydrofuran (R-THMF), the identical AI-2 isomer recognized by LsrB of Salmonella typhimurium. The gene encoding SmlsrB is in an operon with orthologues of the lsr …
Biochemical Characterization Of Two Yeast Paralogous Proteins Mth1 And Std1, Satish Pasula
Biochemical Characterization Of Two Yeast Paralogous Proteins Mth1 And Std1, Satish Pasula
Dissertations
Glucose is the most abundant monosaccharide and preferred carbon and energy source for most cells. Many organisms have evolved sophisticated means to sense glucose and respond to it appropriately. The budding yeast, Saccharomyces cerevisiae senses glucose through two transmembrane proteins, Snf3 and Rgt2. In the presence of extracellular glucose Snf3 and Rgt2 generate an intracellular signal that leads to the degradation of Mthl and Stdl, thereby inducing the expression of hexose transporter genes (EXT) by inhibiting the function of Rgtl, a transcriptional repressor of HXT genes. Mthl and Stdl are degraded via the Yckl/2 Kinase-SCFGrrl-26S proteasome pathway triggered by the …
Characterization Of The Ompl1 Gene Of Pathogenic Leptospira Species In China And Cross-Immunogenicity Of The Ompl1 Protein, Haiyan Dong, Ye Hu, Feng Xue, Dexter Sun, David M. Ojcius, Yafei Mao, Jie Yan
Characterization Of The Ompl1 Gene Of Pathogenic Leptospira Species In China And Cross-Immunogenicity Of The Ompl1 Protein, Haiyan Dong, Ye Hu, Feng Xue, Dexter Sun, David M. Ojcius, Yafei Mao, Jie Yan
All Dugoni School of Dentistry Faculty Articles
Background: The usefulness of available vaccine and serological tests for leptospirosis is limited by the low cross-reactivity of antigens from numerous serovars of pathogenic Leptospira spp. Identification of genus-specific protein antigens (GP-Ag) of Leptospira would be important for development of universal vaccines and serodiagnostic methods. OmpL1, a transmembrane porin of pathogenic leptospires, was identified as a possible GP-Ag, but its sequence diversity and immune cross-reactivity among different serovars of pathogenic leptospires remains largely unknown.
Results: PCR analysis demonstrated that the ompL1 gene existed in all 15 official Chinese standard strains as well as 163 clinical strains of pathogenic leptospires isolated …
Novel Xanthine And Oxanine Dna Glycosylase Activities In Yeast And Mammalian Systems, Liang Dong
Novel Xanthine And Oxanine Dna Glycosylase Activities In Yeast And Mammalian Systems, Liang Dong
All Dissertations
DNA that stores genetic information is frequently damaged in cells. The DNA bases carrying an exocyclic amino group [adenine (A), guanine (G), cytosine (C)] encounter deamination even under physiological conditions. Xanthine (X) and the newly discovered oxanine (O) are derived from deamination of guanine; they are potentially cytotoxic and mutagenic lesions. However, in yeast and eukaryotes, studies on the enzymatic repair of these lesions are limited.
In the yeast, Schizosaccharomyces pombe thymine-DNA glycosylase (Spo TDG) is homologous to human thymine-DNA glycosylase (hTDG), an enzyme that removes thymine from T/G pair. It was reported that Spo TDG contains uracil DNA glycosylase …
Evidence Of A Novel Rna Secondary Structure In The Coding Region Of Hiv-1 Pol Gene., Qi Wang, Ian Barr, Feng Guo, Christopher Lee
Evidence Of A Novel Rna Secondary Structure In The Coding Region Of Hiv-1 Pol Gene., Qi Wang, Ian Barr, Feng Guo, Christopher Lee
Natural Sciences and Mathematics | Faculty Scholarship
RNA secondary structures play several important roles in the human immunodeficiency virus (HIV) life cycle. To assess whether RNA secondary structure might affect the function of the HIV protease and reverse transcriptase genes, which are the main targets of anti-HIV drugs, we applied a series of different computational approaches to detect RNA secondary structures, including thermodynamic RNA folding predictions, synonymous variability analysis, and covariance analysis. Each method independently revealed strong evidence of a novel RNA secondary structure at the junction of the protease and reverse transcriptase genes, consisting of a 107-nucleotide region containing three stems, A, B, and C. First, …
Comparison Of Electrically Mediated And Liposome-Complexed Plasmid Dna Delivery To The Skin, Loree C. Heller, Mark J. Jaroszeski, Domenico Coppola, Richard Heller
Comparison Of Electrically Mediated And Liposome-Complexed Plasmid Dna Delivery To The Skin, Loree C. Heller, Mark J. Jaroszeski, Domenico Coppola, Richard Heller
Bioelectrics Publications
BACKGROUND: Electroporation is an established technique for enhancing plasmid delivery to many tissues in vivo, including the skin. We have previously demonstrated efficient delivery of plasmid DNA to the skin utilizing a custom-built four-plate electrode. The experiments described here further evaluate cutaneous plasmid delivery using in vivo electroporation. Plasmid expression levels are compared to those after liposome mediated delivery.
METHODS: Enhanced electrically-mediated delivery, and less extensively, liposome complexed delivery, of a plasmid encoding the reporter luciferase was tested in rodent skin. Expression kinetics and tissue damage were explored as well as testing in a second rodent model.
RESULTS: Experiments …
Dna Deamination Repair Enzymes In Bacterial And Human Systems, Rongjuan Mi
Dna Deamination Repair Enzymes In Bacterial And Human Systems, Rongjuan Mi
All Dissertations
DNA repair enzymes and pathways are diverse and critical for living cells to maintain correct genetic information. Single-strand-selective monofunctional uracil DNA glycosylase (SMUG1) belongs to Family 3 of the uracil DNA glycosylase superfamily. We report that a bacterial SMUG1 ortholog in Geobacter metallireducens (Gme) and the human SMUG1 enzyme are not only uracil DNA glycosylases (UDG), but also xanthine DNA glycosylases (XDG). Mutations at M57 (M57L) and H210 (H210G, H210M, H210N) can cause substantial reductions in XDG and UDG activities. Increased selectivity is achieved in the A214R mutant of Gme SMUG1 and G60Y completely abolishes XDG and UDG activity. Most …
The Proton-Translocating A Subunit Of F0f1-Atp Synthase Is Allocated Asymmetrically To The Peripheral Stalk., Monika G Düser, Yumin Bi, Nawid Zarrabi, Stanley D Dunn, Michael Börsch
The Proton-Translocating A Subunit Of F0f1-Atp Synthase Is Allocated Asymmetrically To The Peripheral Stalk., Monika G Düser, Yumin Bi, Nawid Zarrabi, Stanley D Dunn, Michael Börsch
Biochemistry Publications
The position of the a subunit of the membrane-integral F0 sector of Escherichia coli ATP synthase was investigated by single molecule fluorescence resonance energy transfer studies utilizing a fusion of enhanced green fluorescent protein to the C terminus of the a subunit and fluorescent labels attached to specific positions of the epsilon or gamma subunits. Three fluorescence resonance energy transfer levels were observed during rotation driven by ATP hydrolysis corresponding to the three resting positions of the rotor subunits, gamma or epsilon, relative to the a subunit of the stator. Comparison of these positions of the rotor sites with those …
Domain Compliance And Elastic Power Transmission In Rotary F(O)F(1)-Atpase., Hendrik Sielaff, Henning Rennekamp, André Wächter, Hao Xie, Florian Hilbers, Katrin Feldbauer, Stanley D Dunn, Siegfried Engelbrecht, Wolfgang Junge
Domain Compliance And Elastic Power Transmission In Rotary F(O)F(1)-Atpase., Hendrik Sielaff, Henning Rennekamp, André Wächter, Hao Xie, Florian Hilbers, Katrin Feldbauer, Stanley D Dunn, Siegfried Engelbrecht, Wolfgang Junge
Biochemistry Publications
The 2 nanomotors of rotary ATP synthase, ionmotive F(O) and chemically active F(1), are mechanically coupled by a central rotor and an eccentric bearing. Both motors rotate, with 3 steps in F(1) and 10-15 in F(O). Simulation by statistical mechanics has revealed that an elastic power transmission is required for a high rate of coupled turnover. Here, we investigate the distribution in the F(O)F(1) structure of compliant and stiff domains. The compliance of certain domains was restricted by engineered disulfide bridges between rotor and stator, and the torsional stiffness (kappa) of unrestricted domains was determined by analyzing their thermal rotary …
Cariporide Prodrugs: Targeting Brain Cancer Cells Through Sodium-Proton Exchange Inhibition, Jacob Vervynckt, Johnathan Brantley, Jessica Moore, Mark Graves Ii, William Harley, Fredric A. Gorin, Hasan Palandoken
Cariporide Prodrugs: Targeting Brain Cancer Cells Through Sodium-Proton Exchange Inhibition, Jacob Vervynckt, Johnathan Brantley, Jessica Moore, Mark Graves Ii, William Harley, Fredric A. Gorin, Hasan Palandoken
Chemistry and Biochemistry
More than 200,000 people in the United States are diagnosed with a primary or metastatic brain tumor annually. The life expectancy for these individuals is approximately 9-12 months from the time of diagnosis. This poor prognosis is due to the ineffectiveness of existing therapies (i.e., chemotherapy and radiotherapy) against brain cancer, where the primary problem is the inability to differentiate cancer cells from healthy brain cells.
Relative to healthy brain tissue, the heightened metabolism of cancer cells increases their reliance on the ion transport proteins NHE (sodium-proton exchanger) and NCX (sodium-calcium exchanger). Inhibition of these proteins disrupts the intricate pH …
Alkoxyamine Polymers: Versatile Materials For Surface Ligation Applications, Lindsey Hines, Jessica Moore, David Dahl, Belinda Lady, Johnathan Brantley, Hasan Palandoken
Alkoxyamine Polymers: Versatile Materials For Surface Ligation Applications, Lindsey Hines, Jessica Moore, David Dahl, Belinda Lady, Johnathan Brantley, Hasan Palandoken
Chemistry and Biochemistry
Immobilization of biomolecules (i.e., proteins, carbohydrates), on polymeric surfaces has been an area of intense research. The resultant bioconjugates often display increased stability, bioavailability and activity. Our research program seeks to explore the utility of the alkoxyamine (RONH2) functional group in new materials as versatile ligating sites for the immobilization of various compounds.
The ease with which alkoxyamines (RONH2) condense with aldehydes or ketones has prompted their widespread use in labelling liposome, bacterial and mammalian cell surfaces as well as chemoselectively ligating small molecule ‘recognition elements' onto polyfunctional substrates. These condensation reactions proceed in aqueous media to afford the robust …
Removal Of Benzaldehyde From Methanol Using Alkoxyamine Functionalized Silica Gel, Dino Sulejmanovic, Carrie Jo Pruitt, Eric Conte, Hasan Palandoken, Shing-Yi Suen
Removal Of Benzaldehyde From Methanol Using Alkoxyamine Functionalized Silica Gel, Dino Sulejmanovic, Carrie Jo Pruitt, Eric Conte, Hasan Palandoken, Shing-Yi Suen
Chemistry and Biochemistry
Alkoxyamines (RONH2) react readily with aldehydes and ketones to form stable oxime ethers, and, thus are expected to be excellent scavengers of these compounds. We installed alkoxyamines on silica gel surface to remove benzaldehyde from methanol. Silica gel was immersed in H2O2/H2SO4 solution to activate the surface. Activated silica gel was then reacted with 3-chloropropyltrichlorosilane. Displacement of the chloride with N-hydroxyphthalimide and subsequent treatment with hydrazine provided alkoxyamines on the surface of silica gel. The resulting silica gel surface with alkoxyamine functional groups was immersed in a 100 ppm benzaldehyde solution …
Molecular Characterisation Of A Bovine-Like Rotavirus Detected From A Giraffe, Emily Mulherin, Jill Bryan, Marijke Beltman, Luke O'Grady, Eugene Pidgeon, Lucie Garon, Andrew Lloyd, John Bainbridge, Helen O'Shea, Paul Whyte, Séamus Fanning
Molecular Characterisation Of A Bovine-Like Rotavirus Detected From A Giraffe, Emily Mulherin, Jill Bryan, Marijke Beltman, Luke O'Grady, Eugene Pidgeon, Lucie Garon, Andrew Lloyd, John Bainbridge, Helen O'Shea, Paul Whyte, Séamus Fanning
Department of Biological Sciences Publications
Background
Rotavirus (RV), is a member of the Reoviridae family and an important etiological agent of acute viral gastroenteritis in the young. Rotaviruses have a wide host range infecting a broad range of animal species, however little is known about rotavirus infection in exotic animals. In this paper we report the first characterisation of a RV strain from a giraffe calf.
Results
This report describes the identification and detailed molecular characterisation of a rotavirus strain detected from a 14-day-old Giraffe (Giraffa camelopardalis), presenting with acute diarrhea. The RV strain detected from the giraffe was characterized molecularly as G10P[11]. …
The Effects Of A Networked Data Acquisition System On Student Learning In General Chemistry Laboratories, Stuart C. Burris, Les L. Pesterfield, Darwin Dahl, Hasan Palandoken, Jacqueline Pope-Tarrence
The Effects Of A Networked Data Acquisition System On Student Learning In General Chemistry Laboratories, Stuart C. Burris, Les L. Pesterfield, Darwin Dahl, Hasan Palandoken, Jacqueline Pope-Tarrence
Chemistry and Biochemistry
We have recently incorporated a networked data acquisition and analysis system into in our second semester general chemistry laboratories. Our investigation team has conducted a study to evaluate the effects of the networked data acquisition system on student performance on a Beer's Law laboratory. We focused on two specific phenomena: the effect of the networked data acquisition system on 1) student learning of specific content material and 2) student comfort level with laboratory work and data analysis. The study involved a networked data acquisition group (employing a UV-Vis spectrometer and colorimeters) and a control group (employing traditional, single wavelength spectrometers). …
Photocycloaddition Of Allenes, Crystal Ward
Photocycloaddition Of Allenes, Crystal Ward
Theses and Dissertations
For the past ten years the Fleming research group has been involved in the research of allene compounds. We have explored the synthesis of allenes as well as several reactions involving allenes, such as oxidation and nitrene addition. Recently, we have explored the synthesis and photochemistry of allenic alcohols tethered to silicon. There are literature examples using allenes in synthesis, but very few examples exist using allenes in photochemical cycloaddition reactions. We have found that a diisopropylallenyloxy(cinnamyloxy)silane derivative undergoes [2+2] photochemical cycloaddition to produce a cyclobutylsilane product when irradiated for 60-75 minutes.
Polymer Supported Optical Biosensors, Ignacio Joseph Garcia
Polymer Supported Optical Biosensors, Ignacio Joseph Garcia
Electronic Theses and Dissertations
The use of various polymer supports adhering phospholipid multibilayers to an internal reflection element have been investigated. The polymer supports studied range from polystyrene (PS), and 100 nm wide PS nanospheres to triethylaminated poly(vinyl benzyl chloride) (PVBC). The PS nanospheres showed the most promise as an adhesion layer since they appear to be the most robust with repeated washings. They also appear to stabilize their adjacent phospholipid multibilayer by increasing the lipid melting temperature. The triethylaminated DVBC also provided an increase of lipid melting temperature, but not quite to the same degree as the PS nanospheres. The cooperativity of such …
Tethering Polypeptides Through Bifunctional Peg Cross-Linking Agents To Probe Protein Function: Application To Atp Synthase., Daniel J Cipriano, Stanley D Dunn
Tethering Polypeptides Through Bifunctional Peg Cross-Linking Agents To Probe Protein Function: Application To Atp Synthase., Daniel J Cipriano, Stanley D Dunn
Biochemistry Publications
Chemical crosslinking mediated by short bifunctional reagents has been widely used for determining physical relationships among polypeptides in multisubunit proteins, but less often for functional studies. Here we introduce the approach of tethering polypeptides by using bifunctional reagents containing a lengthy, flexible PEG linker as a form of crosslinking especially suited to functional analyses. The rotary molecular motor ATP synthase was used as a model subject. Single cysteine residues were introduced into selected positions of ATP synthase epsilon subunit, a component of the rotor subcomplex of the enzyme, and the unrelated maltose binding protein (MBP), then the two purified recombinant …
An Inositolphosphorylceramide Synthase Is Involved In Regulation Of Plant Programmed Cell Death Associated With Defense In Arabidopsis, Wenming Wang, Xiaohua Yang, Samantha Tangchiaburana, Roland Ndeh, Jennifer E. Markham, Yoseph Tsegaye, Teresa M. Dunn, Guo-Liang Wang, Maria Bellizzi, James F. Parsons, Danielle Morrissey, Janis E. Bravo, Daniel V. Lynch, Shunyuan Xiao
An Inositolphosphorylceramide Synthase Is Involved In Regulation Of Plant Programmed Cell Death Associated With Defense In Arabidopsis, Wenming Wang, Xiaohua Yang, Samantha Tangchiaburana, Roland Ndeh, Jennifer E. Markham, Yoseph Tsegaye, Teresa M. Dunn, Guo-Liang Wang, Maria Bellizzi, James F. Parsons, Danielle Morrissey, Janis E. Bravo, Daniel V. Lynch, Shunyuan Xiao
Department of Biochemistry: Faculty Publications
The Arabidopsis thaliana resistance gene RPW8 triggers the hypersensitive response (HR) to restrict powdery mildew infection via the salicylic acid–dependent signaling pathway. To further understand how RPW8 signaling is regulated, we have conducted a genetic screen to identify mutations enhancing RPW8-mediated HR-like cell death (designated erh). Here, we report the isolation and characterization of the Arabidopsis erh1 mutant, in which the At2g37940 locus is knocked out by a T-DNA insertion. Loss of function of ERH1 results in salicylic acid accumulation, enhanced transcription of RPW8 and RPW8-dependent spontaneous HR-like cell death in leaf tissues, and reduction in plant stature. …
Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last
Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last
Yan Lu
No abstract provided.
Global Analysis Of Gene Expression Changes During Retinoic Acid-Induced Growth Arrest And Differentiation Of Melanoma: Comparison To Differentially Expressed Genes In Melanocytes Vs Melanoma, Mary H. Estler, Goran Boskovic, James Denvir, Sarah Miles, Donald A. Primerano, Richard M. Niles
Global Analysis Of Gene Expression Changes During Retinoic Acid-Induced Growth Arrest And Differentiation Of Melanoma: Comparison To Differentially Expressed Genes In Melanocytes Vs Melanoma, Mary H. Estler, Goran Boskovic, James Denvir, Sarah Miles, Donald A. Primerano, Richard M. Niles
Biochemistry and Microbiology
BACKGROUND: The incidence of malignant melanoma has significantly increased over the last decade. Some of these malignancies are susceptible to the growth inhibitory and pro-differentiating effects of all-trans-retinoic acid (RA). The molecular changes responsible for the biological activity of RA in melanoma are not well understood.
RESULTS: In an analysis of sequential global gene expression changes during a 4-48 h RA treatment of B16 mouse melanoma cells, we found that RA increased the expression of 757 genes and decreased the expression of 737 genes. We also compared the gene expression profile (no RA treatment) between non-malignant melan-a mouse melanocytes and …
Simple Inhibitors Of Histone Deacetylase Activity That Combine Features Of Short-Chain Fatty Acid And Hydroxamic Acid Inhibitors, Jessica L. Tischler, Basel Abuaita, Sierra C. Cuthpert, Christopher Fage, Kristi Murphy, Andrew Saxe, Edward B. Furr, Jamie Hedrick, Jennifer Meyers, David Snare, Ali R. Zand
Simple Inhibitors Of Histone Deacetylase Activity That Combine Features Of Short-Chain Fatty Acid And Hydroxamic Acid Inhibitors, Jessica L. Tischler, Basel Abuaita, Sierra C. Cuthpert, Christopher Fage, Kristi Murphy, Andrew Saxe, Edward B. Furr, Jamie Hedrick, Jennifer Meyers, David Snare, Ali R. Zand
Chemistry & Biochemistry Publications
Butyric acid and trichostatin A (TSA) are anti-cancer compounds that cause the upregulation of genes involved in differentiation and cell cycle regulation by inhibiting histone deacetylase (HDAC) activity. In this study we have synthesized and evaluated compounds that combine the bioavailability of short-chain fatty acids, like butyric acid, with the bidentate binding ability of TSA. A series of analogs were made to examine the effects of chain length, simple aromatic cap groups, and substituted hydroxamates on the compounds' ability to inhibit rat-liver HDAC using a fluorometric assay. In keeping with previous structure-activity relationships, the most effective inhibitors consisted of longer …
Molecular-Orientation-Dependent Ac Stark Effect And Its Impact On Multiphoton Processes, Xi Chu
Molecular-Orientation-Dependent Ac Stark Effect And Its Impact On Multiphoton Processes, Xi Chu
Chemistry and Biochemistry Faculty Publications
We study the dependence of the ac Stark shifts of electronic energies on the molecular orientation relative to the polarization direction of an incident intense laser field, using a three-dimensional non-Hermitian Floquet method and H(2)+ as a model system. Simultaneously, we also study the orientation-dependent high-order harmonic generation (HHG) and multiphoton ionization (MPI). We find that with the presence of near-one-photon resonance, the Stark effect strongly mixes electronic states of different symmetries to create quasienergy states (QESs). The orientation dependence of multiphoton processes, in which these QESs play an important role, becomes complex. Population transfer is better achieved with aligned …
Phosphorylation Of P27kip1 Regulates Assembly And Activation Of Cyclin D1-Cdk4, Michelle D. Larrea, Jiyong Liang, Thiago G. Da Silva, Feng Hong, Shan H. Shao, Kathy Han, D. Dumont, Joyce M. Slingerland
Phosphorylation Of P27kip1 Regulates Assembly And Activation Of Cyclin D1-Cdk4, Michelle D. Larrea, Jiyong Liang, Thiago G. Da Silva, Feng Hong, Shan H. Shao, Kathy Han, D. Dumont, Joyce M. Slingerland
Chemistry and Physics Faculty Articles
p27 mediates Cdk2 inhibition and is also found in cyclin D1-Cdk4 complexes. The present data support a role for p27 in the assembly of D-type cyclin-Cdk complexes and indicate that both cyclin D1-Cdk4-p27 assembly and kinase activation are regulated by p27 phosphorylation. Prior work showed that p27 can be phosphorylated by protein kinase B/Akt (PKB/Akt) at T157 and T198. Here we show that PKB activation and the appearance of p27pT157 and p27pT198 precede p27-cyclin D1-Cdk4 assembly in early G1. PI3K/PKB inhibition rapidly reduced p27pT157 and p27pT198 and dissociated cellular p27-cyclin D1-Cdk4. Mutant p27 allele products lacking phosphorylation at …