Life Sciences Commons^™

Neutralization-Sensitive R5 Shiv-2873nip Encoding Env From A Infant With Recent Hiv Clade C Infection, Nagadenahalli B. Siddappa, Ruijiang Song, Victor G. Kramer, Agnes-Laurence Chenine, Vijayakumar Velu, Helena Ong, Robert A. Rasmussen, Ricky D. Grisson, Charles Wood, Hong Zhang, Chipepo Kankasa, Rama Rao Amara, James G. Else, Francis J. Novembre, David Montefiori, Ruth M. Ruprecht

Nebraska Center for Virology: Faculty Publications

HIV-C (HIV-C) accounts for >56% of all HIV infections worldwide. To investigate vaccine safety and efficacy in non-human primates, a pathogenic, R5-tropic, neutralization sensitive simian-human immunodeficiency virus (SHIV) encoding HIV-C env would be desirable. We have constructed SHIV-2873Ni, an R5 SHIV encoding a primary pediatric HIV-C env isolated from a 2-month old Zambian infant, who progressed to death within one year of birth. SHIV-2873Ni was constructed using SHIV-1157ipd3N4 (Song et al., J. Viol. 80:8729-38, 2006) as backbone since the latter contains additional NF-κB sites in the long terminal repeats (LTRs) to enhance viral replicative capacity. The parental virus, SHIV-2873Ni, was …

Domain Compliance And Elastic Power Transmission In Rotary F(O)F(1)-Atpase., Hendrik Sielaff, Henning Rennekamp, André Wächter, Hao Xie, Florian Hilbers, Katrin Feldbauer, Stanley D Dunn, Siegfried Engelbrecht, Wolfgang Junge

Biochemistry Publications

The 2 nanomotors of rotary ATP synthase, ionmotive F(O) and chemically active F(1), are mechanically coupled by a central rotor and an eccentric bearing. Both motors rotate, with 3 steps in F(1) and 10-15 in F(O). Simulation by statistical mechanics has revealed that an elastic power transmission is required for a high rate of coupled turnover. Here, we investigate the distribution in the F(O)F(1) structure of compliant and stiff domains. The compliance of certain domains was restricted by engineered disulfide bridges between rotor and stator, and the torsional stiffness (kappa) of unrestricted domains was determined by analyzing their thermal rotary …

Finding Recurrent Regions Of Copy Number Variation: A Review, Oscar M. Rueda, Ramon Diaz-Uriarte

COBRA Preprint Series

Copy number variation (CNV) in genomic DNA is linked to a variety of human diseases, and array-based CGH (aCGH) is currently the main technology to locate CNVs. Although many methods have been developed to analyze aCGH from a single array/subject, disease-critical genes are more likely to be found in regions that are common or recurrent among subjects. Unfortunately, finding recurrent CNV regions remains a challenge. We review existing methods for the identification of recurrent CNV regions. The working definition of ``common'' or ``recurrent'' region differs between methods, leading to approaches that use different types of input (discretized output from a …

Smokejumper Obituary: Stanley, Philip B. (Missoula 1943), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Alkoxyamine Polymers: Versatile Materials For Surface Ligation Applications, Lindsey Hines, Jessica Moore, David Dahl, Belinda Lady, Johnathan Brantley, Hasan Palandoken

Chemistry and Biochemistry

Immobilization of biomolecules (i.e., proteins, carbohydrates), on polymeric surfaces has been an area of intense research. The resultant bioconjugates often display increased stability, bioavailability and activity. Our research program seeks to explore the utility of the alkoxyamine (RONH2) functional group in new materials as versatile ligating sites for the immobilization of various compounds.

The ease with which alkoxyamines (RONH2) condense with aldehydes or ketones has prompted their widespread use in labelling liposome, bacterial and mammalian cell surfaces as well as chemoselectively ligating small molecule ‘recognition elements' onto polyfunctional substrates. These condensation reactions proceed in aqueous media to afford the robust …

Cariporide Prodrugs: Targeting Brain Cancer Cells Through Sodium-Proton Exchange Inhibition, Jacob Vervynckt, Johnathan Brantley, Jessica Moore, Mark Graves Ii, William Harley, Fredric A. Gorin, Hasan Palandoken

Chemistry and Biochemistry

More than 200,000 people in the United States are diagnosed with a primary or metastatic brain tumor annually. The life expectancy for these individuals is approximately 9-12 months from the time of diagnosis. This poor prognosis is due to the ineffectiveness of existing therapies (i.e., chemotherapy and radiotherapy) against brain cancer, where the primary problem is the inability to differentiate cancer cells from healthy brain cells.

Relative to healthy brain tissue, the heightened metabolism of cancer cells increases their reliance on the ion transport proteins NHE (sodium-proton exchanger) and NCX (sodium-calcium exchanger). Inhibition of these proteins disrupts the intricate pH …

Apoyo Del Intsormil Incrementa La Produccion Y Productividad Del Cultivo De Sorgo En America Central, Intsormil

INTSORMIL Impacts and Bulletins

El INSORMIL ha apoyado, las actividades de investigación y transferencia de tecnología de sorgo desde 1981, con énfasis en Nicaragua y El Salvador pero en los ultimos años se viene expandiendo a otros países de America Central. Estadísticas del Ministerio de Agricultura y Ganadería de El Salvador, indican un signifi cativo incremento en la producción nacional de grano de sorgo en la misma superfi cie sembrada desde hace 9 años, debido principalmente al incremento en el rendimiento por unidad de superfi cie, tal como se presenta en el gráfi co. En este gráfi co puede verse que desde 1999 al …

Neuronostatin Encoded By The Somatostatin Gene Regulates Neuronal, Cardiovascular, And Metabolic Functions, Willis K. Samson, Jian V. Zhang, Orna Avsian-Kretchmer, Kai Cui, Gina L.C. Yosten, Cindy Klein, Rong Ming Lyu, Xiong Wang Yong, Qun Chen Xiang, Jun Yang, Christopher J. Price, Ted D. Hoyda, Alastair V. Ferguson, Xiao Bin Yuan

Biological Sciences Faculty Research & Creative Works

Somatostatin is important in the regulation of diverse neuroendocrine functions. Based on bioinformatic analyses of evolutionarily conserved sequences, we predicted another peptide hormone in pro-somatostatin and named it neuronostatin. Immuno-affinity purification allowed the sequencing of an amidated neuronostatin peptide of 13 residues from porcine tissues. In vivo treatment with neuronostatin induced c-Fos expression in gastrointestinal tissues, anterior pituitary, cerebellum, and hippocampus. In vitro treatment with neuronostatin promoted the migration of cerebellar granule cells and elicited direct depolarizing actions on paraventricular neurons in hypothalamic slices. In a gastric tumor cell line, neuronostatin induced c-Fos expression, stimulated SRE reporter activity, and promoted …

Fisheries Occasional Publication No.72 - Western Rock Lobster Ecological Effects Of Fishing Research Plan., Rhys Brown

Fisheries Occasional Publications

The commercial West Coast Rock Lobster Fishery (WCRLF) targets the western rock lobster (WRL), Panulirus cygnus, averaging approximately 10,000 tonnes of lobsters each year. Currently approximately 450 to 500 boats operate in the Fishery in waters ranging from 5 m to 200 m depth adjacent to the Western Australian coast and stretching from Cape Leeuwin in the south to Shark Bay in the north. The Fishery is managed by the Department of Fisheries (DoF) in Western Australia, using an input control system designed to constrain exploitation/fishing effort and deliver sustainable catches. The annual value of the catch is estimated at …

Expressional Control Of A Cadmium-Transporting P1b-Type Atpase By A Metal Sensing Degradation Signal, David J. Adle, Jaekwon Lee

Department of Biochemistry: Faculty Publications

Cadmium is a highly toxic environmental contaminant implicated in various diseases. Our previous data demonstrated that Pca1, a P_1B-type ATPase, plays a critical role in cadmium resistance in yeast S. cerevisiae by extruding intracellular cadmium. This illustrates the first cadmium-specific efflux pump in eukaryotes. In response to cadmium, yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. To gain mechanistic insights into the cadmium-dependent control of Pca1 expression, we have characterized the pathway for Pca1 turnover and the mechanism of cadmium sensing that leads to up-regulation of Pca1. Pca1 is a short-lived protein (t_½ < 5 min) and is subject to ubiquitination when cells are growing in media lacking cadmium. Distinct from many plasma membrane transporters targeted to the vacuole for degradation via endocytosis, cells defective in this pathway did not stabilize Pca1. Rather, Pca1 turnover was dependent on the proteasome. These data suggest that, in the absence of cadmium, Pca1 is targeted for degradation before reaching the plasma membrane. Mapping of the N terminus of Pca1 identified a metal-responding degradation signal encompassing amino acids 250–350. Fusion of this domain to a stable protein demonstrated that it functions autonomously in a metal-responsive manner. Cadmium sensing by cysteine residues within this domain circumvents ubiquitination and degradation of Pca1. These data reveal a new mechanism for substrate-mediated control of P_{1B …}

Pseudomonas Aeruginosa-Candida Albicans Interactions: Localization And Fungal Toxicity Of A Phenazine Derivative, Jane Gibson, Arpanah Sood, Deborah A. Hogan

Dartmouth Scholarship

Phenazines are redox-active small molecules that play significant roles in the interactions between pseudomonads and diverse eukaryotes, including fungi. When Pseudomonas aeruginosa and Candida albicans were cocultured on solid medium, a red pigmentation developed that was dependent on P. aeruginosa phenazine biosynthetic genes. Through a genetic screen in combination with biochemical experiments, it was found that a P. aeruginosa-produced precursor to pyocyanin, proposed to be 5-methyl-phenazinium-1-carboxylate (5MPCA), was necessary for the formation of the red pigmentation. The 5MPCA-derived pigment was found to accumulate exclusively within fungal cells, where it retained the ability to be reversibly oxidized and reduced, and its …

Removal Of Benzaldehyde From Methanol Using Alkoxyamine Functionalized Silica Gel, Dino Sulejmanovic, Carrie Jo Pruitt, Eric Conte, Hasan Palandoken, Shing-Yi Suen

Chemistry and Biochemistry

Alkoxyamines (RONH₂) react readily with aldehydes and ketones to form stable oxime ethers, and, thus are expected to be excellent scavengers of these compounds. We installed alkoxyamines on silica gel surface to remove benzaldehyde from methanol. Silica gel was immersed in H₂O₂/H₂SO₄ solution to activate the surface. Activated silica gel was then reacted with 3-chloropropyltrichlorosilane. Displacement of the chloride with N-hydroxyphthalimide and subsequent treatment with hydrazine provided alkoxyamines on the surface of silica gel. The resulting silica gel surface with alkoxyamine functional groups was immersed in a 100 ppm benzaldehyde solution …

(2008) Northwood Stomwater Technologies And Town Of Northwood Buffer Ordinance, Ttg Environmental Consultants, Llc

PREP Reports & Publications

TTG Environmental Consultants, LLC (TEC), under contract to the New Hampshire Estuaries Project and in conjunction with the Northwood Water Resources Sub-Committee, has prepared this report on stormwater technologies appropriate for the Town of Northwood, NH. This report is divided into two (2) main sections with a number of subsections. The first section describes the need for stormwater management, and the second section describes stormwater management technologies that are appropriate for Northwood. Stormwater management has been evolving for many years, from a need to convey stormwater away from or through a developed site, to the realization that land development has …

Molecular Characterisation Of A Bovine-Like Rotavirus Detected From A Giraffe, Emily Mulherin, Jill Bryan, Marijke Beltman, Luke O'Grady, Eugene Pidgeon, Lucie Garon, Andrew Lloyd, John Bainbridge, Helen O'Shea, Paul Whyte, Séamus Fanning

Department of Biological Sciences Publications

Background

Rotavirus (RV), is a member of the Reoviridae family and an important etiological agent of acute viral gastroenteritis in the young. Rotaviruses have a wide host range infecting a broad range of animal species, however little is known about rotavirus infection in exotic animals. In this paper we report the first characterisation of a RV strain from a giraffe calf.

Results

This report describes the identification and detailed molecular characterisation of a rotavirus strain detected from a 14-day-old Giraffe (Giraffa camelopardalis), presenting with acute diarrhea. The RV strain detected from the giraffe was characterized molecularly as G10P[11]. …

Annette Dupard, Annette Dupard, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Annette Dupard is a native of Vacherie, Louisiana. She is a graduate of Southern University and a retired Food Service Manager for the East Baton Rouge Parish Child Nutrition Programs.

Kris M. Chustz, Kris M. Chustz, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Kris M. Chustz, a native of Baton Rouge, Louisiana, is a recently retired school food service professional, having worked as a Manager, Area Supervisor, and Computer Training Coordinator for the Eastern Baton Rouge Parish.

Emma Jo Williamson, Emma Jo Williamson, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Emma Jo Williamson’s first job after college was in social work before making a career change and teaching Home Economics at Plaquemine High School in Iberville Parish for thirteen years. She then decided to go into school food service. She began her new career as an Assistant Director of the parish for two years before assuming the position of Director. Emma Jo is finally retired after twenty-seven years of serving the children of Iberville Parish.

Threats To The Robert B. Gordon Area For Environmental Studies (Gna), West Chester University Of Pennsylvania

Gordon Natural Area History & Strategic Plan Documents

No abstract provided.

Intsormil Support Contributes To Increased Sorghum Yields In Central America, Intsormil

INTSORMIL Impacts and Bulletins

INTSORMIL has supported sorghum research and technology transfer activities in Central America since 1981. Emphasis has been on El Salvador and Nicaragua but now the program is expanding to other Central American countries. Statistics provided by the Ministry of Agriculture indicate a significant advance in sorghum production in El Salvador. As shown in the graph below surface area in ha sown to sorghum during the period 1999-2009 did not increase (about 105,000 ha). However, during that same period grain production increased from 140,000 MT to 186,000 MT, an increase of 33%. This is due to the dramatic yield increase per …

Transcriptome Analysis Identifies Novel Responses And Potential Regulatory Genes Involved In Seasonal Dormancy Transitions Of Leafy Spurge (Euphorbia Esula L.), David P. Horvath, Wun S. Chao, Jeffrey C. Suttle, Jyothi Thimmapuram, James V. Anderson

Cyber Center Publications

Dormancy of buds is a critical developmental process that allows perennial plants to survive extreme seasonal variations in climate. Dormancy transitions in underground crown buds of the model herbaceous perennial weed leafy spurge were investigated using a 23 K element cDNA microarray. These data represent the first large-scale transcriptome analysis of dormancy in underground buds of an herbaceous perennial species. Crown buds collected monthly from August through December, over a five year period, were used to monitor the changes in the transcriptome during dormancy transitions.

Mary Davis-Palms, Mary Davis-Palms, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Mary Davis-Palms is an Area Supervisor with the East Baton Rough Parish School Nutrition Programs. A graduate of Louisiana State University, Mary has thirty-four years of service in child nutrition.

Nadine Mann, Nadine Mann, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Louisiana native Dr. Nadine Mann is the Director of Operations for the East Baton Rouge Parish Child Nutrition Programs. Nadine holds Bachelor’s and Master’s degrees from Louisiana State University and a Doctorate from Texas Woman’s University in Foods and Nutrition Institution Administration. She has been with the district for thirty-one years.

Gail Johnson, Gail Johnson, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Gail Johnson was the longtime director of school food service in East Baton Rough Parish, Louisiana. She was also a very active member of the Advisory Committee for the National School Food Service Management Institute/Institute of Child Nutrition.

Mary T. Wroten, Mary T. Wroten, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Mary Wroten, a native of Mississippi, worked in the East Baton Rouge Parish Child Nutrition Programs for twenty years. Among her many accomplishments while there was to greatly improve the procurement procedures for the district. Mary’s legacy will continue in Dietetics when her daughter graduates in the same field in May 2009.

The Effects Of A Networked Data Acquisition System On Student Learning In General Chemistry Laboratories, Stuart C. Burris, Les L. Pesterfield, Darwin Dahl, Hasan Palandoken, Jacqueline Pope-Tarrence

Chemistry and Biochemistry

We have recently incorporated a networked data acquisition and analysis system into in our second semester general chemistry laboratories. Our investigation team has conducted a study to evaluate the effects of the networked data acquisition system on student performance on a Beer's Law laboratory. We focused on two specific phenomena: the effect of the networked data acquisition system on 1) student learning of specific content material and 2) student comfort level with laboratory work and data analysis. The study involved a networked data acquisition group (employing a UV-Vis spectrometer and colorimeters) and a control group (employing traditional, single wavelength spectrometers). …

Sylvia Grabert, Sylvia Grabert, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Sylvia Grabert is a retired Food Service Director for Iberia Parish in Louisiana. Before coming to Iberia Parish, Sylvia founded the first school food service program in St. James Parish. She holds degrees in both Education and Dietetics, and taught school for several years before going into school food service.

Altos Costos Del Trigo: El Caso De La Harina De Sorgo En La Industria De La Panifi Cacion De El Salvador, Intsormil

INTSORMIL Impacts and Bulletins

El programa de tecnología de alimentos del CENTA, con el apoyo del INTSORMIL ha estado desarrollando recetas de pan a base de harina de sorgo y probándolas en pequeñas y medianas panaderías de El Salvador por muchos años. Ahora con los precios de harina de trigo a $0.57 por libra, los panaderos de El Salvador han demostrado un creciente interés en el uso del sorgo a un costo de $0.32 por libra como un sustituto parcial de la harina de trigo en sus productos. Los tecnólogos de alimentos del CENTA han estado promoviendo el uso y consumo de harina de …

Timing Of Supplementation Of Selenium And Isoflavones Determines Prostate Cancer Risk Factor Reduction In Rats, Merrill J. Christensen, Jessica R. Tolman, Edwin D. Lephart, Dennis L. Eggett, Kenneth Setchell

Faculty Publications

High dietary intake of selenium or isoflavones reduces risk factors for prostate cancer. We tested whether combined supplementation of these two dietary components would reduce prostate cancer risk factors in rats more than supplementation of each component individually. Methods Male Noble rat pups were exposed from conception to diets containing an adequate (0.33-0.45 mg/kg diet) or high (3.33-3.45 mg/kg) concentration of selenium as Se-methylselenocysteine and a low (10 mg/kg) or high (600 mg/kg) level of isoflavones in a 2 x 2 factorial design. Pups consumed their respective diets until sacrifice at 35, 100, or 200 days. Male Noble rat breeders, …

Prevalence And Factors Associated With Intestinal Parasitic Infection Among Children In An Urban Slum Of Karachi., Vikram Mehraj, Juanita Hatcher, Saeed Akhtar, Ghazala Rafique, Mohammad Asim Beg

Department of Pathology and Laboratory Medicine

Background:Intestinal parasitic infections are endemic worldwide and have been described as constituting the greatest single worldwide cause of illness and disease. Poverty, illiteracy, poor hygiene, lack of access to potable water and hot and humid tropical climate are the factors associated with intestinal parasitic infections. The study aimed to estimate prevalence and identify factors associated with intestinal parasitic infections among 1 to 5 years old children residing in an urban slum of Karachi Pakistan. Methods And Principal
Findings:A cross sectional survey was conducted from February to June 2006 in Ghosia Colony Gulshan Town Karachi, Pakistan. A simple random …

Improving Reversal Median Computation Using Commuting Reversals And Cycle Information, William Arndt, Jijun Tang

Faculty Publications

In the past decade, genome rearrangements have attracted increasing attention from both biologists and computer scientists as a new type of data for phylogenetic analysis. Methods for reconstructing phylogeny from genome rearrangements include distance-based methods, MCMC methods, and direct optimization methods. The latter, pioneered by Sankoff and extended with the software suites GRAPPA and MGR, is the most accurate approach, but is very limited due to the difficulty of its scoring procedure—it must solve multiple instances of the reversal median problem to compute the score of a given tree. The reversal median problem is known to be NP-hard and all …