Life Sciences Commons^™

Signaling Mechanisms For Muscarinic Receptor-Mediated Coronary Vasoconstriction In Isolated Rat Hearts, Yi Zhang

Electronic Theses and Dissertations

The signaling mechanisms for muscarinic receptor-mediated vasoconstriction in coronary resistance arteries were studied in KCl-arrested isolated rat hearts perfused at a constant flow rate. The cholinergic agonists acetylcholine and bethanechol were given by bolus injection or constant infusion. The coronary vascular resistance was monitored by measuring the changes in perfusion pressure. The selective muscarinic agonist bethanechol caused a similar vasoconstrictor response as ACh, but with less potency and efficacy. Bolus injection of bethanechol evoked a phasic vasoconstriction in a dose-dependent manner, while infusion of bethanechol evoked a tonic vasoconstriction without producing tachyphylaxis. Coronary vascular responses to bethanechol were further examined …

Reactive Oxygen Species Generated By Depletion Of Cellular Glutathione Mediate Cadmium Cytotoxicity, Jun Xu

Masters Theses

Studies were put forth to test whether or not reactive oxygen species, which are influenced by the cellular level of reduced glutathione, mediate the toxicity of cadmium. Cadmium has been a focal point in the health care community in the past few years because it has been shown to induce the development of tumors, interfere with cellular signaling, and cause damage to organs, tissues, and biomolecules. Although various studies and theories have been proposed, little direct evidence, especially from studies utilizing primary cells in culture, has accumulated to show the mechanism by which the cadmium induced toxicity occurs.

Our studies …

Progress Rural Western Australia Conference : A Conference Focusing On How To Bring Out The Best In Your Community, 13 And 14 August 1999, New Norcia, Western Australia : Conference Proceedings, Progress Rural Western Australia

All other publications

The Objective

The 1999 Progress Rural WA Conference aims to build on the skills and exposure that many delegates have already had to inspiring and innovative practice from across the globe and the excellent practice currently being implemented by rural West Australians.

Our keynote speakers have been selected on their ability to paint the big picture - we want to heighten the excitement, push people beyond their comfort zones and stretch peoples thinking.

From project workshops and feedback across the state, we hear a number of common issues. The workshop themes have been developed to provide an action learning experience …

Genetic And Structural Analyses Of Cytoplasmic Filaments Of Wild-Type Treponema Phagedenis And A Flagellar Filament-Deficient Mutant, Jacques Izard, William A. Samsonoff, Mary Beth Kinoshita, Ronald J. Limberger

Department of Food Science and Technology: Faculty Publications

Unique cytoplasmic filaments are found in the treponeme genus of spirochete bacteria. Their function is unknown, but their location underneath the periplasmic flagellar filaments (PFF) suggests a role in motility and/or cell structure. To better understand these unique structures, the gene coding for the cytoplasmic filaments, cfpA, was identified in various treponemal species. Treponema phagedenis cfpA was 2,037 nucleotides long, and the encoded polypeptide showed 78 to 100% amino acid sequence identity with the partial sequence of CfpA from T. denticola, T. vincentii, and T. pallidum subsp. pertenue. Wild-type T. phagedenis and a …

Deliberate Introductions Of Species: Research Needs, John J. Ewel, Dennis J. O'Dowd, Joy Bergelson, Curtis C. Daehler, Carla M. D'Antonio, Luis Diego Gómez, Doria R. Gordon, Richard J. Hobbs, Alan Holt, Keith R. Hopper, Colin E. Hughes, Marcy Lahart, Roger R. B. Leakey, William G. Lee, Lloyd L. Loope, David H. Lorence, Svata M. Louda, Ariel E. Lugo, Peter B. Mcevoy, David M. Richardson, Peter M. Vitousek

Svata M. Louda Publications

Research questions about introductions

Several research questions need to be answered to help ensure that proposed introductions are done wisely and safely.

Guarding against risks without sacrificing benefits:
How can the potential benefits and costs of introductions best be evaluated in economic, environmental, and social terms?
Should all introductions be regulated?
How different must organisms or recipient ecosystems be from those assessed previously to warrant independent assessment?
When is it appropriate to assess and regulate taxa other than species?
What are appropriate ecological and political boundaries for regulation?

Alternatives to introductions:
How and when can indigenous organisms be domesticated so …

Effect Of Various Starch Sources On The Silicone Holdout Of Release Paper, Chandrasekaran Duraiswamy

Masters Theses

Siliconized papers are widely being used from food and packaging industry to pressure sensitive label industry and their use has increased considerably over the last decade. Surface and structural properties of the sheet influence the silicone hold out and release properties.

Surface sizing of the sheet is done prior to silicone coating. The objective of this research is to find their film forming capabilities, fluid hold out of various starches, and the effect of the various calendering process on the release properties was also studied.

No significant difference between the starch sources were observed, except for the modified com starch …

Systematics Of The Eucestoda: Advances Toward A New Phylogenetic Paradigm And Observations On The Early Diversification Of Tapeworms And Vertebrates, Eric P. Hoberg, Scott Lyell Gardner, Ronald A. Campbell

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Evolutionary relationships of the Eucestoda have received intense but sporadic attention over the past century. Since 1996, the landscape has dramatically changed with respect to our knowledge of the phylogenetic relationships among the tapeworms. The 2nd International Workshop for Tapeworm Systematics (IWTS) held in Lincoln, Nebraska in October of that year provided the catalyst for development of novel hypotheses for inter-and intra-ordinal phylogeny. The working-group structure of the 2nd IWTS and results of phylogenetic studies are briefly introduced in the present manuscript. Higher-level phylogenies derived from parsimony analysis of independent data bases representing comparative morphology or molecular sequences were largely …

Role Of Mitogen-Activated Kinases In Cd40-Mediated T Cell Activation Of Monocyte/Macrophage And Vascular Smooth Muscle Cell Cytokine/Chemokine Production, Denise M. Milhorn

Electronic Theses and Dissertations

This dissertation represents efforts to determine the functional consequences acquired by vascular smooth muscle cells (SMC) in response to CD40 ligation by activated CD154+ T cells, and to elucidate components of the signaling pathway(s) activated in response to CD40 signaling in both monocytes and SMC. To study the consequences of CD40 stimulation, primary human monocytes and aortic SMC were treated with plasma membranes purified from CD154 + , CD4+ T cells. The results presented in this dissertation demonstrate that SMC, like monocytes/macrophages, are capable of interacting with T cells in a manner that results in reciprocal activation events. SMC were …

Avian Communities Of Large Reclaimed Mine Grasslands In Southwestern Indiana, Travis L. Devault

All-Inclusive List of Electronic Theses and Dissertations

Many bird species native to the tallgrnss prairie region oCthc central U.S. have declined drastically since European settlement due lo the destruction and alteration of grassland habitat. In this study, the breeding bird communities or 21 large reclaimed surface coal mine grasslands in a seven-county area or southwestern Indiana were examined with respect to species and guild composition, density, variation among sites, and grassland size. In addition, the vegetation characteristics of nine large mine grasslands were described and related to the presence and abundance or eight grasslund bird species common to the study areas. The immense size of mine grasslands …

Universal Design And Nature Trails: Balancing Accessibility, Site Integrity, And The Recreation Experience, Steve Kirkindall

Electronic Theses and Dissertations

This study addressed the application of Americans With Disabilities Act (ADA) guidelines in outdoor recreation settings. The purpose was three-fold: to develop site- and user-friendly design principles for non-urban, accessible nature trails; to design and construct a trail based on those principles; and to evaluate both principles and trail by surveying trail users. The Delphi Method was employed with a national panel to assess current expert opinion regarding accessibility and trails. Fifty-nine principles were developed from combined results of panel deliberations and applied experience gained constructing a prototype trail utilizing the universal design concept. Trail users rated the importance of …

Succinate Dehydrogenase (Sdh) From Bradyrhizobium Japonicum Is Closely Related To Mitochondrial Sdh, David J. Westenberg, Mary Lou Guerinot

Dartmouth Scholarship

The sdhCDAB operon, encoding succinate dehydrogenase, was cloned from the soybean symbiont Bradyrhizobium japonicum. Sdh from B. japonicum is phylogenetically related to Sdh from mitochondria. This is the first example of a mitochondrion-like Sdh functionally expressed in Escherichia coli.

The Prairie Naturalist Volume 31, No.3 September 1999

The Prairie Naturalist

HANTA VIRUS INFECTION IN NORTH DAKOTA SMALL MAMMALS: 1994,1995 ▪ R. W. Seabloom, J. J. Feist, and S L. McDonough

AMERICAN WOODCOCK IN COLORADO ▪ C. E. Braun

AVIFAUNA OF AN EARLY SUCCESSIONAL HABITAT ALONG THE MIDDLE MISSOURI RIVER ▪ D. L. Swanson

REPRODUCTIVE STATUS OF FLEA BEETLES IN NORTH DAKOTA AND SOUTH DAKOTA ▪ M. A. Brinkman and S. A. Clay

NUTRIENT AND ENERGY CHARACTERISTICS OF INVERTEBRATES FROM TWO LOCATIONS IN KANSAS ▪ S. G. Papon, R. J. Robel, and K. Kemp

BOOK REVIEWS

The Wood Warblers ▪ M. G. Knutson

A Fascination With Birds. ▪ J. A. Dechant …

Use Of Extractable Lipofuscin For Age Determination Of Blue Crab Callinectes Sapidus, Se-Jong Ju, David H. Secor, H. Rodger Harvey

OES Faculty Publications

The blue crab Callinectes sapidus is an economically and ecologically important species in many temperate estuaries, yet stock assessments have been limited to length-based methods for demographic analyses. We evaluated the potential of age pigments (lipofuscins) sequestered in neural tissue of eye-stalks and brains to estimate the age of blue crabs collected from Chesapeake Bay and Chincoteague Bay. The rate of lipofuscin accumulation was determined using crabs of known age reared in the laboratory. Age pigments were extracted from neural tissues (eye-stalk or brain), quantified, and normalized to protein content to allow comparisons across tissue types and crab sizes. Field-collected …

The Salinity And Hydrology Of The Upper Slab Hut Catchment, R Ferdowsian, A T. Ryder

Resource management technical reports

No abstract provided.

Maritime Pine For Western Australia - Revegetation On Farms Information Kit, David Bicknell, Department Of Conservation And Land Management

Natural resources published reports

Revegetation on farms

The Revegetation on Farms Project and the material in this Information Kit is targeted at the agricultural zone of Western Australia with less than 600 mm rainfall. Some of the information is valid outside this range.

All information is provided in good faith, to help make informed revegetation decisions leading to sustainable land use.

TreeNote No 20 - Weed control in eucalypts and pines

TreeNote No 21 - Insect pests of eucalypts and pines

In search of the perfect pine - David Gough (Article)

Farming carbon - Syd Shea (Article)

Two-Metal-Ion Catalysis In Adenylyl Cyclase, John J.G. Tesmer, Roger K. Sunahara, Roger A. Johnson, Gilles Gosselin, Alfred G. Gilman, Stephen R. Sprang

Biological Sciences Faculty Publications

Adenylyl cyclase (AC) converts adenosine triphosphate (ATP) to cyclic adenosine monophosphate, a ubiquitous second messenger that regulates many cellular functions. Recent structural studies have revealed much about the structure and function of mammalian AC but have not fully defined its active site or catalytic mechanism. Four crystal structures were determined of the catalytic domains of AC in complex with two different ATP analogs and various divalent metal ions. These structures provide a model for the enzyme- substrate complex and conclusively demonstrate that two metal ions bind in the active site. The similarity of the active site of AC to those …

A Pilot Study Of Exercise Training To Reduce Trunk Fat In Adults With Hiv-Associated Fat Redistribution, Ronenn Roubenoff, Lauren Weiss, Ann Mcdermott, Tanya Heflin, Gregory J. Cloutier, Michael Wood, Sherwood Gorbach

Kinesiology and Public Health

Body fat redistribution (‘lipodystrophy’), with gain in abdominal and trunk fat, and decline in facial and limb fat, is a newly recognized problem in patients with HIV infection that has been linked to use of HIV-1 protease inhibitors. Increased abdominal fat may predispose these patients to hypertension, diabetes, and coronary artery disease. At this time no effective treatment is available. We examined whether exercise training could reduce trunk fat in men with fat redistribution.

Phemeranthus Rugospermus (Holz.) Kiger, Janice Coons

Specimens by Name

No abstract provided.

Kyrt1, A Disarmed Version Of A Highly Tumorigenic Agrobacterium Tumefaciens Strain Identified As Chry5, Rebecca S. Torisky, Glenn B. Collins

Plant and Soil Sciences Faculty Patents

Disarmed A. tumefaciencs strain KYRT1, derived from a highly tumorigenic strain identified as A. tumefaciens strain Chry5. Disarming is accomplished by inactivation of plasmid pTiChry5 T-DNA sequences by, for example, deletion of sequences comprising the T-DNA right border. Methods of making transgenic plants using the novel A. tumefaciens strains are also provided.

Cropwatch No. 99-19, July 23, 1999, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field updates………….168

Insect thresholds………….169

Effects of short crop………….169

Wheat yields………….169

Soybean leaf cupping………….170

Insect update………….171

Dicamba injury………….172

Rust in beans………….173

Sunflower moths………….173

Precipitation…………. 174

Some Thoughts About The Number And Size Of Farms In Nebraska, Roy Frederick

Cornhusker Economics

The 1997 Census of Agriculture indicated that Nebraska had 51,454 farms at the end of that year. (A farm is a place from which $1,000 or more of agricultural products are sold annually). This is the smallest number of farms since early in the state抯 history.

Agricultural Land Values In South Dakota: A Comparison Of Methods And Findings From Two Surveys: 1995-1999, Larry Janssen

Economics Research Reports (1969 - 2017)

This report provides comparative findings from two South Dakota farmland market value surveys (SDSU and SDASS) for the 1995 - 1999 period. The annual SDSU farmland market survey is a key informant mail survey of agricultural appraisers, lenders, and extension educators. The annual SDASS county-level farmland survey results are based on nearly 3300 telephone survey responses from a random sample of farmers and ranchers. This report also includes considerable documentation of methods used to generate land value estimates at the regional and statewide level from available data in both surveys. The results and discussion sections include comparisons of: (1) relative …

Historical Evolution Of Crop Systems In Eastern South Dakota: Economic Influences, Linda Dumke, Thomas L. Dobbs

Economics Research Reports (1969 - 2017)

Cropping systems in the United States and throughout much of the world have moved toward shorter and less diverse rotations during the last half of the twentieth century. However, as we approach the new millennium, there is growing concern about the ecological sustainability of monocultures and such narrow rotations as the com-soybean rotation. Problems of pest control, crop disease, groundwater contamination from chemical pesticides and fertilizers, and soil erosion are proving to be very difficult when crop rotation systems lack diversity. "Silver bullet" technologies sometimes buy time, but without diversity, new ecological problems soon replace the problem just "solved" with …

Hübner’S Helicta, The Forgotten Neonympha: The Recognition And Elevation Of Neonympha Helicta (Nymphalidae: Satyrinae) To Specific Status, The Designation Of Neotypes For N. Helicta And N. Areolatus, The Subspecific Transfer Of Septentrionalis To Helicta And The Description Of A Third Helicta Subspecies From South Florida, Ronald R. Gatrelle

The Taxonomic Report of the International Lepidoptera Survey

Hübner (1806) based the name Oreas fimbriata helicta on a John Abbot painting of a southeastern United States satyr without a written description. Subsequent lepidopterists have nearly ignored this taxon. Helicta is herein recognized as a valid species–Neonympha helicta. A neotype is designated for helicta from Aiken County, South Carolina and deposited in the Allyn Museum of Entomology Sarasota, Florida. A neotype is also designated for Papilio areolata J.E. Smith, 1797 from Chatham County, Georia and deposited in the Allyn Museum of Entomology. Septentrionalis Davis, 1924 is recognized as the northern United States subspecies of helicta not areolatus …

Lysimachia Quadriflora Sims, Jennifer Ward

Specimens by Name

No abstract provided.

Phyla Lanceolata (Michx.) Greene, Jennifer Ward

Specimens by Name

No abstract provided.

Phyla Lanceolata (Michx.) Greene, Jennifer Ward

Specimens by Name

No abstract provided.

Are The Market Lows In For Wheat?, Lynn Lutgen

Cornhusker Economics

On July 12, 1999, the United States Department of Agriculture released its crop production report. The report indicated that all wheat production is down nine percent from 1998. The 1999 United States wheat crop is now estimated at 2.333 billion bushels, which is 217 million less than the 1998 harvest.

Are The Market Lows In For Wheat?, Lynn Lutgen

Cornhusker Economics

On July 12, 1999 USDA released it’s crop production report. The report indicated that all wheat production is down nine percent from 1998. The 1999 U.S. wheat crop is now estimated at 2.333 billion bushels, which is 217 million less than the 1998 harvest.

Posttranscriptional Regulation Of Mammalian Methionine Synthase By B12, Sumedha Gulati, Lawrence C. Brody, Ruma V. Banerjee

Department of Biochemistry: Faculty Publications

Methionine synthase is one of two key enzymes involved in the removal of the metabolite, homocysteine. Elevated homocysteine levels constitute a risk factor for cardiovascular diseases and for neural tube defects. In cell culture, the activity of methionine synthase is enhanced several-fold by supplementation with its cofactor, B₁₂. The mechanism of this regulation is unknown, although it has been ascribed to a shift from apoenzyme to holoenzyme. Using sensitive assay techniques as well as a combination of Northern and Western analyses, we demonstrate that the effect of B,, on induction of methionine synthase activity is paralleled by an …