Life Sciences Commons^™

Analytical Description Of X-Ray Peaks: Application To L X-Ray Spectra Processing Of Lanthanide Elements By Means Of The Electron Probe Micro-Analyzer, G. Remond, Ph. Coutures, C. Gilles, D. Massiot

Scanning Microscopy

The shape of Lα X-ray peaks analyzed by means of a LiF (200 plane) monochromator was described by a pseudo-Voigt function:

P(λ) = 0.35 P₁(λ)+ 0.65 P₂(λ)

where P₁(λ) and P₂(λ) are a Gaussian and a Lorentzian distribution centered at the same wavelength, with the same amplitude and half-width and in relative proportion 0.35 and 0.65 respectively. For peaks occurring at wavelength greater than ≃ 0.17 nm, a Gaussian offset was added in order to correct the asymmetry of peaks resulting from the monochromator mounting within the spectrometer.

The effective wavelength resolution …

Scanning Electron Microscope-Based Metrological Electron Microscope System And New Prototype Scanning Electron Microscope Magnification Standard, Michael T. Postek

Scanning Microscopy

A metrological electron microscope has been developed at the National Institute of Standards and Technology (NIST) traceable to national standards of length, and a new prototype magnification standard meeting the current needs of the scanning electron microscope (SEM) user community has been fabricated. This metrology instrument is designed to certify standards for the calibration of the magnification of the SEM and for the certification of artifacts for linewidth measurement done in the SEM. The artifacts will be useful for various applications in which the SEM is currently being used. The SEM-based metrology system is now operational at the Institute, and …

Electron Microscopy Of Lipid Deposits In Human Atherosclerosis, G. Pasquinelli, P. Preda, M. Vici, M. Gargiulo, A. Stella, M. D'Addato, R. Laschi

Scanning Microscopy

The filipin probe associated with tannic acid stain was used to study intra-and extracellular lipids in surgically removed human atherosclerotic lesions (n = 20). In particular, intimal thickenings, fatty streaks and fibrolipidic plaques have been investigated by using mainly transmission and scanning electron microscopy. In the intimal thickenings, the lipid deposits were mainly localized in the subendothelial space as homogeneously sized particles (40-140 nm) and more heterogeneous uni-multilamellar vesicles (35-700 nm). Intermediate lipid forms were also observed. In the fatty streaks, the lipid deposits were intracellular and mainly observed in cells with a monocyte/macrophagic phenotype. Lipid inclusions, lipid lysosomal bodies …

Changes In The Esophageal Epithelium In Rabbits Treated By Cis-Dichlorodiammineplatinum As Studied By Electron Microscopy, M. Albertsson, C. -H. Hakansson, M. Palmegren

Scanning Microscopy

The esophageal mucosa of the rabbit was investigated after a single dose of 5 mg Cis-Dichlorodiammineplatinum (Cis-DDP). Specimens were taken for scanning electron microscopy, transmission electron microscopy, and light microscopy. Examination was performed daily for 20 consecutive days. A cytotoxic effect was observed already the first day after injection with an intracellular oedema. Thereafter the height of the esophageal epithelium and the basal cell layer steadily decreased to a minimum day 11. This parallels the damaged microridges and an increased cell loss as revealed by scanning electron microscopy. At the end of the observation period the esophageal mucosa had completely …

The Effect Of 10 And 20 Gy Single Dose Irradiation On The Esophageal Mucosa Of The Rabbit. An Electron Microscopic Study, C. H. Hakansson, M. Albertsson, M. Palmegren

Scanning Microscopy

The mucosa of rabbit esophagus was irradiated with single doses of 10 and 20 Gy respectively. Specimens were taken for scanning electron microscopy (SEM), and light microscopy investigations. Examinations were made 1-14 days after 10 Gy and 1-17 days after 20 Gy. Irradiation resulted in edema during the first days. The thickness of the epithelium was at its lowest value around day 5 after the administration of 10 Gy, and on days eight to ten after the administration of 20 Gy. Damage of the epithelium surface, as scored on SEM micrographs, was most pronounced on days eight to eleven after …

Scanning Electron Microscopy Of Chromosomes And Chromosome Fragments In Transgenic Rainbow Trout, Jane E. Disney, Christine M. Davitt, Gary H. Thorgaard

Scanning Microscopy

Chromosomes and chromosome fragments from embryonic offspring of a transgenic rainbow trout were examined using scanning electron microscopy (SEM). SEM is an extremely useful technique for studying the structure of chromosome fragments since little morphological detail is revealed by conventional staining methodologies and light microscopy. The chromosome preparations were processed for SEM by combining an osmium-thiocarbohydrazide-osmium (OTO) technique with 2-4 nm of gold deposition. This technique revealed the organization of individual chromatin fibers in chromosome fragments and intact chromosomes. Both a linear chromosome fragment with a width similar to that of an intact chromatid (approximately 0.60 micrometers) and a spherical …

The Physical State Of Potassium In Frog Skeletal Muscle Studied By Ion-Sensitive Microelectrodes And By Electron Microscopy: Interpretation Of Seemingly Incompatible Results, L. Edelmann

Scanning Microscopy

According to the commonly accepted membrane pump theory most of cellular K⁺ ions are freely dissolved in free cellular water; the alternative association-induction hypothesis postulates that the bulk of cellular K⁺ is adsorbed (weakly bound) to cellular proteins which are maintained in a specific labile state in the cytoplasm of a living cell. K⁺ activities measured with ion-sensitive microelectrodes in the cytoplasm of frog skeletal muscle seem to confirm the claim that most of cellular K⁺ ions are free in cellular water. On the other hand, it is evident from electron microscopic ion binding studies that …

Scanning Electron Microscopy Of The Small Intestine Mucosa In Children With Celiac Disease After Long-Term Dietary Treatment, M. Morroni, A. Sbarbati, G. D'Angelo, C. Catassi, P. L. Giorgi, S. Cinti

Scanning Microscopy

Jejunal mucosal specimens from twenty children with celiac disease were studied by light microscopy (LM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM) after one year of dietary treatment. An ultrastructural morphometric study was performed in five patients who had an intestinal permeability (IP) test. Seventeen patients were tested for serum antigliadin antibodies (AGA). In ten children, in whom LM showed partial villous atrophy, SEM and TEM examination confirmed the lesion. In the second group (10 children) with normal morphology at routine LM, SEM showed lesions of variable degree in 70% of cases. The morphological ultrastructural investigation showed good …

A Summary Of Continuing Research With Non Native Oysters At The Virginia Institute Of Marine Science, Roger L. Mann

Reports

No abstract provided.

A Scanning Electron Microscopy Analysis Of Human Cytotoxic Cell Subsets And Of Their Mode Of Conjugation With Tumor Cell Targets, Walter Malorni, Giuseppe Arancia, Francesca Iosi, Gianfranco Donelli, Daniela Zarcone, Alessandro Moretta, Carlo Enrico Grossi

Scanning Microscopy

In this study we describe the surface features of non-MHC (Major Histocompatibility Complex)-restricted cytotoxic cells isolated from human peripheral blood. Purified populations of CD3^-, natural killer (NK) cells were allowed to interact with NK-sensitive (K562) and NK-resistant (THP-1-0) tumor cell targets. The type of effector to target cell binding was investigated by scanning electron microscope (SEM) analysis. A different interaction with the effectors is described for NK-resistant targets in comparison with NK-susceptible tumor cells. SEM was also used to investigate the relationship between interleukin 2 (IL2)-activated cytotoxic cells (lymphokine-activated killer, LAK, cells) and the tumor targets. We also …

Interview With Craig Allen Smith (Fa 107), Manuscripts & Folklife Archives

FA Oral Histories

Transcription of interview with Craig Allen Smith conducted by Jeffrey Brian Hagan on 3 November 1989. From folk studies student project titled “Farming Folklore,” concerning farming traditions and practices in Kentucky.

Scanning Electron Microscope With A Single-Polepiece Lens, V. Kolařík, I. Müllerová, M. Lenc

Scanning Microscopy

The design of an ultra-high vacuum scanning electron microscope (UHV SEM) with a single-pole-piece lens underneath the specimen is described with the possibility to guide backscattered (BSE) and secondary electrons (SE) which originate in the magnetic field of the single-polepiece lens to the detectors. Our new design of the single-pole-piece lens and in-lens deflection coils closely satisfy the condition of a variable axis immersion lens (VAIL), which results in very low deflection aberrations.

Early Phase Changes During The Setting Of High Copper Amalgams, T. Ruzickova, J. Had

Scanning Microscopy

X-ray diffraction analysis has been used to evaluate the phase composition of two (spherical and lathe cut) HCSC amalgam alloys and early phase changes in these amalgam in the time interval 2.5 min to 7 days after trituration. A method appropriate for the evaluation of these changes was developed. Only two phases were found in alloys Valiant and Ana 2000: gamma(Ag₃Sn) and epsilon(Cu₃Sn). In amalgams, no diffraction peaks were observed corresponding to gamma 2 phase in the time interval form 2.5 min to 7 days after trituration. The relative concentration changes of the reaction products of …

Can Genetically Engineered Crops Become Weeds?, Kathleen H. Keeler

School of Biological Sciences: Faculty Publications

There are significant differences if the distribution of weedy characteristics among weeds, normal plants, and crops. The world’s most serious weeds possess on the average 10 or 11 of these characters, a random collection of British plants have an average seven of the traits, and crop plants only five. For the average crop to become as “weedy” as the average weed, it would need to acquire five weedy traits. Even using the unlikely assumption that those traits are single loci in which a dominant mutation would provide the weedy character, this would require the simultaneous acquisition of five gene substitutions. …

Soil Disturbance-Residue Management Effect On Winter Wheat Growth And Yield, Wallace Wilhelm, H. Bouzerzour, J. F. Power

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The need to reduce soil erosion, maximize soil water conservation, and optimize grain production in dryland cropping systems in the Central Great Plains has culminated in development of nontilled fallow systems. These systems have greatly reduced the degree of soil disturbance, and the amount and degree of residue incorporation. The objectives of this study were to evaluate the influence of soil disturbance and residue management on soil temperature, soil water, and winter wheat (Triticum aestivum L.) growth. Two field studies were established in 1981 and 1982 on an Alliance silt loam (Fine-silty, mixed, mesic Aridic Argiustoll) with treatments consisting …

Interview With William M. Cecil (Fa 107), Manuscripts & Folklife Archives

FA Oral Histories

Transcription of interview with William M. Cecil conducted by Jeffrey Brian Hagan on 1 November 1989. From folk studies student project titled “Farming Folklore,” concerning farming traditions and practices in Kentucky.

Rock Lobster Industry Advisory Committee Report To The Hon Minister For Fisheries., Fisheries Dept. Of Western Australia

Fisheries management papers

To ensure full consultation with members of the fishing industry, the Committee arranged a series of meetings at the main coastal centres after the close of the fishing season. Each meeting followed the same format which allowed matters related to research and management to be discussed. After these meetings the Committee met to formulate its advice to the Minister on the matters raised during the Coastal Tour and from Committee discussions.

Fishing Licences As Security For Loans., Fisheries Department Of Western Australia.

Fisheries management papers

During mid 1988 the Minister for Fisheries at that time, the Hon Julian Grill, sought the establishment of a committee of advice to examine security arrangements for financial institutions providing funds for fishing licences and fishing boats. The prime objective of the Committee was to examine and report upon the requirements for establishing fishing licences as a form of collateral for loans. The major issue before the Committee arose from licence holders seeding generally the means by which their investment in a fishing licence could be used as security, whereas lending institutions sought to ensure their loans would be adequately …

Crop And Livestock Enterprises, Risk Evaluation, And Management Strategies On South Dakota Sustainable Farms, Donald Taylor, Thomas L. Dobbs, David L. Becker, James D. Smolik

Economics Research Reports

This report presents findings from personal interviews undertaken in January-March 1989 with 22 of the 32 sustainable/regenerative farmers in South Dakota who responded to a Summer 1988 mail survey concerning their sustainable farming practices. [For the mail survey results, see Taylor, Dobbs, and Smolik, 1989.] The major purpose of the personal interview part of the study reported herein was to gain greater insight into (1) the sustainable crop rotations and livestock enterprises on these farmers' farms and (2) the judgments of these farmers about the riskiness and managerial strategies for meeting selected challenges of sustainable agriculture.

Presence Of A Cs-Resistant Transient Outward Current Which Distorts Ica Measurements In Guinea-Pig Single Ventricular Cells, Georges Christé, Brigitte Delachapelle, Fouad Lemtiri-Chlieh, Carlos Ojeda

fouad Lemtiri-Chlieh

To correctlyr analyse the physical properties of ion channels and the actions of drugs upon them, it is important that the membrane conducts only the ionic species believed permeant. In guinea-pig ventricular cells it has been assumed that only ICaL flows in the potential range -50 to +60 mV in the presence of intra- and extracellular Cs (> 20 mM). We report here that this is not the case due to the presence of a Cs-insensitive, transient outward current.

Measurements Of Cytosolic Free Ca In Cultured Rat Neonate And Adult Guinea-Pig Ventricular Cardiac Myocytes, Jean-Claude Bernengo, Fouad Lemtiri-Chlieh, Carlos Ojeda, Nadine Pltonoff

fouad Lemtiri-Chlieh

Cytosolic free Ca was measured with Indo-1 (Grynkiewicz et al. 1985) as free acid (in guinea-pig cells) or as the ester (in rat neonate cells). Using image analysis we obtained concentration maps at 40 ms intervals at two wavelengths (Cannell et al. 1986). In rat neonate cells, at rest and during contraction, calcium was apparently non-uniformly distributed, in contrast to guinea-pig adult cells. Using a dual PM spectroscopic system adapted to an inverted microscope we found: (1) the conversion of Indo-AM to Indo- 1 in the cytosol is not complete (< 40% contribution to the total light); (2) rat neonate cells buffer Ca (at rest 130-170 nM) only for [Ca]. between 1 and 3 mM, whereas in adult guinea-pig cells the range extended to 10 mM; (3) during contraction [Ca]i rose from 200 nm to no more than 1 microM. Digitoxin increases basal and peak [Ca]i; (4) during spontaneous contractions, in guinea-pig cells, [Ca]i rises from 70 to 200 nm when [Ca]o = 0, and from 170 nm to about 1 microM when [Ca]o = 2 mM; (5) in voltage-clamp conditions the rise in [Ca]i does not exceed 2 microM and oscillations occur in the presence of forskolin. When loading with Indo-1, cells frequently cease to contract (Powell et al. 1988) and the variations in [Ca]i are then very small (about 200 nm at peak ICa). In both cases these values are small compared to the total charge crossing the membrane.

Pig Vocalizations Under Selected Husbandry Practices, Hongwei Xin, James A. Deshazer, D. W. Leger

Hongwei Xin

Acoustical characteristics of vocalizations of sows, piglets, and nursery pigs under selected husbandry practices were analyzed with a digital signal processing system. The duration (D) and major energy-resonance frequency (P) for each call were determined as follows: a) processing of piglets (D = 0.81 s and f* = 3 700 Hz); b) food anticipation of breeding-gestation sows (2.50 s and 3 000 Hz); c) isolation of piglet (0.34 s and 500, 3 500 Hz); d) startling of nursery pigs (0.29 s and 900 Hz); e) sows in heat (3.07 s and 1 375 Hz); f) farrowing (0.10 s and 3 …

Kentucky Warbler (Vol. 65, No. 4), Kentucky Library Research Collections

Kentucky Warbler

No abstract provided.

Complex Motor Dynamics And Control In Multi-Looped Negative Feedback Systems, Anne Beuter, John Milton, Christiane Labrie, Leon Glass

WM Keck Science Faculty Papers

Experiments were performed in normal and Parkinsonian subjects who were asked to maintain a constant finger position using time-delayed visual feedback. The finger position showed complex dynamics, with characteristic differences between normal and Parkinosonian subjects. Here we discuss some of the theoretical issues that must be resolved in order to understand the mechanisms that underlie the generation of these complex signals.

Linker Insertion Mutants Of Simian Virus 40 Large T Antigen That Show Trans-Dominant Interference With Wild-Type Large T Antigen Map To Multiple Sites Within The T-Antigen Gene., Jiyue Y. Zhu, Charles N. Cole

Dartmouth Scholarship

Linker insertion mutants affecting the simian virus 40 (SV40) large tumor (T) antigen were constructed by inserting a 12-base-pair oligonucleotide linker into restriction endonuclease cleavage sites located within the early region of SV40. One mutant, with the insertion at amino acid 5, was viable in CV-1p and BSC-1 cells, indicating that sequences very close to the amino terminus of large T could be altered without affecting the lytic infection cycle of SV40. All other mutants affecting large T were not viable. In complementation assays between the linker insertion mutants and either a late-gene mutant, dlBC865, or a host range/helper function …

Muscle Activities That Stretch The Mind, James L. Hanegan, Heather Mckean

Biology Faculty Publications

No abstract provided.

An Alternative Approach For Evaluating The Efficacy Of Potential Biocontrol Agents Of Weeds. 2. Path Analysis, Dan J. Pantone, William A. Williams, Armand R. Maggenti

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Path analysis was used to assess the efficacy of the fiddleneck flower gall nematode as a weed biocontrol agent of coast fiddleneck in competition with wheat during two years of field experiments. The path analysis revealed that the number of inflorescences/plant for fiddleneck and the number of heads/plant for wheat were the most important yield components that determine fecundity and seed yield. The density of fiddleneck had a much greater impact on the yield components of fiddleneck than did the density of wheat or the nematode rate of inoculation. The nematode had its greatest negative impact on the number of …

Detection In Soil Of A Deletion In An Engineered Dna Sequence By Using Dna Probes, Janet K. Jansson, William E. Holben, James M. Tiedje

Biological Sciences Faculty Publications

Two Pseudomonas strains were engineered to contain the nptII gene and plasmid vector sequences in their chromosomes. After incubation of these strains in nonsterile soil, total bacterial DNA was isolated and analyzed by Southern blot hybridization with the nptII gene and the plasmid vector as probes. In addition to the expected bands of hybridization, a new band corresponding to the loss of vector sequences from the chromosome while retaining the nptII gene was observed for one of the strains. The more stressful conditions encountered in soil appeared to increase the frequency of loss of the vector sequences …

Hagan, Jeffrey Brian (Fa 107), Manuscripts & Folklife Archives

FA Finding Aids

Finding aid and full-text scan of paper (Click on “Additional Files” below) for Folklife Archives Project 107. Farming folklore. Interviews, November 1, 3, 1989, with William M. Cecil and Craig Allen Smith concerning farm occupational lore and duties. Includes paper discussing collector's fieldwork experiences.

Evaluation Of Automated Threshold Selection Methods For Accurately Sizing Microscopic Fluorescent Cells By Image-Analysis, Me Sieracki, Se Reichenbach, Kl Webb

VIMS Articles

The accurate measurement of bacterial and protistan cell biomass is necessary for understanding their population and trophic dynamics in nature. Direct measurement of fluorescently stained cells is often the method of choice. The tedium of making such measurements visually on the large numbers of cells required has prompted the use of automatic image analysis for this purpose. Accurate measurements by image analysis require an accurate, reliable method of segmenting the image, that is, distinguishing the brightly fluorescing cells from a dark background. This is commonly done by visually choosing a threshold intensity value which most closely coincides with the outline …