Life Sciences Commons^™

Effects Of Acidic Precipitation On The Soil Chemistry And Bioavailability Of Aluminum, Manganese, And Copper, Alan Christie Stam

Doctoral Dissertations

The effects of acidic precipitation on the soil solution chemistry and bioavailability of AI, Mn, and Cu in soils from spruce-fir and oak-hickory forests were examined in laboratory, greenhouse, and field studies.

Soil solution concentrations of labile (inorganic) monomeric AI (LMAI) increased with decreasing solution pH and with increasing exchangeable AI and decreasing exchangeable Ca/AI of the soils. Dissolved organic matter decreased with decreasing solution pH and influenced decreases in nonlabile (organic) monomeric AI (NLMAI).

Simulated acidic precipitation increased LMAI concentrations in the laboratory equilibration and greenhouse studies, but not in the field plot studies in oak-hickory and spruce-fir forests. …

Prediction Of Release Properties Of Silicone Coated Paper Through Analysis Of Coating Particle Size And Distribution, Kent Bartholomew

Paper Engineering Senior Theses

Silicone release coatings are gaining in popularity. The use of water-borne emulsions is seeing its way into the production of release coatings as well as pressure sensitive applications.

Thermoset silicone release coatings systems consist of a reactive polymer, a crosslinker, and a catalyst. A condensation reaction takes place to form the low surface energy silicone film which is primarily what gives good release property.

It is believed that an emulsion's stability, and hence quality, can be related to the particle size and distribution.

It was the goal of this study to quantify the relationship between particle size/distribution and release properties …

An Analysis Of The Wash And Flotation Deinking Processes, Brian L. Felgner

Paper Engineering Senior Theses

The purpose of this paper is to obtain data, so that we may compare the ability of Floatation vs. Wash deinking to produce a paper of equal standards as the original unprinted base-stock.

It was determined that both flotation and wash deinked stock with the addition of 40% fresh pulp can produce a paper of equal physical and optical properties as the unprinted stock from which it was made.

Floatation deinked stock was found have a higher materials cost, however, it displayed better strength and opacity properties than wash deinked stock. The difference is thought to result from a large …

Site-Directed Mutagenesis Of The Putative Processing Site Of Recombinant Human Immunodeficiency Virus Reverse Transcriptase, Susan M. Poppe

Masters Theses

The human immunodeficiency virus (HIV) is the etiological agent of the acquired immune deficiency syndrome (AIDS). This retrovirus, encodes a RNA dependent DNA polymerase, reverse transcriptase (RT), which is essential to virus replication. Biochemical analysis indicated that RT is composed of 2 polypeptides of 66,000 and 51,000 daltons (p66 and p51) which combine to form a heterodimer. This heterodimer is thought to arise from the post-translational processing where p66 is made and either autocatalytic or proteolytic cleavage events result in a heterogeneous p66/p51. Site-directed mutagenesis was used at the proposed processing site to probe HIV-RT processing.

Three prokaryotic expression vectors …

Effect Of Disulfiram On The Urinary Excretion Of 5-Hydroxytryptophol And 5-Hydroxyindoleacetic Acid In Subjects Consuming Serotonin Rich Foods, Amy G. Tordecilla

Loma Linda University Electronic Theses, Dissertations & Projects

The effect of disulfiram and/or serotonin rich foods (SRF) on the urinary excretion of 5-hydroxytryptophol (5-HTOL) and 5-hydroxyindoleacetic acid (5-HIAA) in normal male subjects was investigated. Urine collections were obtained on 1) regular serotonin free diet, 2) SRF, 3) serotonin free diet with disulfiram, and 4) SRF with disulfiram. Urinary excretion of 5-HTOL in ug/gm creatinine during disulfiram administration was 112.0 ± 24.1. Five HTOL (ug/gm creatinine) with disulfiram and SRF increased to 1532 ± 352.0 (p < 0.001). Five-HIAA (mg/g creatinine) before and after SRF was 3.4 ± .44 and 33.1 ± 6.1 respectively, p < 0.001. With disulfiram and SRF the excretion of was 24.2 ± 3.2 mg/g creatinine which was significantly greater than when on the regular diet (p < 0.001) than the excretion on SRF alone (p < 0.05)

Key words: Disulfiram, serotonin, 5-hydroxytryptophol, 5-hydroxyindoleacetic acid, urinary excretion, high performance liquid chromatography, alcoholism

Hourly Fluctuation Of Middle Ear Pressure As A Function Of Age In School-Age Children, Susan Hogue Henry

Dissertations and Theses

Tympanometry is a useful means of evaluating the status of the middle ear. For the pediatric population, tympanometry is particularly valuable for determining the presence of middle ear effusion. The test has been incorporated in many school hearing conservation programs because of its ease of administration, objectivity, and diagnostic value.

In a study by deJonge and Cummings (1985), the hourly fluctuation of middle ear pressure was reported in a group of kindergarten-age children. The variability of middle ear pressure for that group of children averaged 150 daPa. In the present study, a maturational effect of this hourly fluctuation was observed …

Regulation Of Queuine Insertion Into Transfer Rna: Effects Of Tumor Promoters, Bonnie Jean Brooks

Chemistry & Biochemistry Theses & Dissertations

The purpose of this study was to treat normal human fibroblasts with known tumor promoters and observe queuine modification of tRNA. The queuine insertion enzyme, tRNAguanine ribosyltransferase, was studied in vivo and in vitro. Tumor promoter-treated human fibroblast cultures exhibited variable queuine-insertion rates with a transient inhibition that correlated with variable levels of queuine modified tRNA over time in culture from passages 3 — 8. In contrast the in vitro studies showed that phorbol cetera and saccharin actually increased insertion with strong evidence indicating phosphorylation as a positive modulating force of the enzyme activity. It is proposed that chronic stimulation …

The Determination Of Tamoxifen Citrate In Human Blood Serum And Semen, Karimdad Tahmoresi

Chemistry & Biochemistry Theses & Dissertations

High performance liquid chromatography (HPLC)methodologies for the determination of tamoxifen and its metabolites 4-hydroxytamoxifen and n-desmethyltamoxifen, and using cis-tamoxifen as an internal standard have been developed.

Clinical analyses of tamoxifen and its metabolites in human serum and semen, were performed as follows: The analytes were extracted from serum and semen with hexane, the hexane evaporated, the dried extract then reconstituted with methanol, followed by reverse phase high performance liquid chromatography.

Between-run coefficients of variation (CV'S) for tamoxifen and n-desmethyltamoxifen for serum sample analyses, (n = 15) were, 4% and 13% at mean (SD) concentration of 140.2 (6.2) and 71.8 (9.8) …

Electrostatic Effects On Airborne Asbestos Monitoring, Roxanne Francis

Biological Sciences Theses & Dissertations

The affects of electrostatic forces on the Membrane Filter Method (MFM) used for air-borne asbestos monitoring have been investigated for many years. Several studies have indicated that these forces interfere with the collection of asbestos fibers on the membrane filter, which results in an underestimated analysis of the airborne concentration. By varying the electroconductivity of the extension cowls on the filter cassettes, it was speculated that the significance of the electrostatic forces could be statistically analyzed. Four sets of filter cassettes with grounded conductive (GC) extension cowls, ungrounded conductive (UC) extension cowls, grounded nonconductive (GN) extension cowls, and ungrounded nonconductive …

Changes In Caloric Content As A Result Of Sub-Lethal Stress In Neanthes Virens (Sars), Phyllis J. Friello

Biological Sciences Theses & Dissertations

Neanthes virens were subjected to a toxicity test with 1.8, 3.4, 6.7 and 11.0 mg/1 Cd²⁺. Worms were withdrawn on Days 1, 4, 7, 10 and 14 from each of five replicate tanks and their caloric content determined by bomb calorimetry. Analyses of variance and covariance showed no significant differences in worm caloric content with withdrawal day or cadmium concentration.

In a second experimental series, a sediment toxicity test with Neanthes virens was conducted in contaminated sediments from two sites, as well as reference sediments from a control site. Caloric content of the worms was measured by bomb …

Studies On The Giant Mealie Witchweed, Striga Forbesii Benth., In Zimbabwe, David Allen Knepper

Biological Sciences Theses & Dissertations

Of the seven species of Striga found in Zimbabwe, S. forbesii Benth. is currently the most destructive in terms of crop damage. Owing to its limited occurrence worldwide as a pest of crops, relatively little work has been done on this species.

Striga forbesii has been found to be a consistent problem on both maize and sorghum in the Hunter's Road District of KweKwe, Zimbabwe. Results of a pot study and field observations, indicate S. forbesii can successfully parasitize Urochloa panicoides Beauv., Sorghum arundinaceum (Desv.) Stapf, and Rottboellia cochinchinensis (L.) L.f., all of which are widespread weeds of arable land. …

Effects Of Dietary Corn Oil And Menhaden Oil On Rats Mammary Tumorigenesis And Prostaglandin E2 Levels, Melissa L. Philley

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Arts and Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Melissa L. Philley on August 4, 1989.

Hypercholesterolemia And Platelet Sensitivity To Thromb In-Induced Aggregation In Whole Blood, Charles E. Hammond Ii

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Arts and Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Sciences in Biology by Charles E. Hammond, II on August 1, 1989.

Frequency Of Rickettsia Sps. In Dermacentor Variabilis And Amblyomma Americanum In Central Hanover County, Virginia, Peggy Ann Keefe

Master's Theses

In the eastern United States, Dermacentor variabilis is the primary vector of Rickettsia rickettsii, the etiological agent for Rocky Mountain Spotted Fever. Cases of Rocky Mountain Spotted Fever in eastern United States increased steadily from 1959 to 1979 to a peak of one case per 200,000 people in 1979 (Morbidity and Mortality Weekly Report, 1985). In 1976, 98.9% of the reported cases of Rocky Mountain Spotted Fever were east of the 100th meridian with 1.1% occurring west of the 100th meridian (Burgdorfer, 1977). Hanover County, Virginia is an endemic area for this disease (Sonenshine, 1971); the last reported case was …

The Genus Marasmius From The Southern Appalachian Mountains, Dennis Edmund Desjardin

Doctoral Dissertations

The genus Marasmius (Tricholomataceae, Agaricales, Basidiomycotina) constitutes one of the larger genera of litter-decomposing and litter-binding mushrooms, represented worldwide by approximately 500 species. Thirty-eight taxa representing eight sections of the genus are recognized as occurring in the southern Appalachian Mountains. Seven of these taxa are new to science, while an additional 14 are reported for the first time from the study area. In addition, one new section is proposed.

Descriptions of southern Appalachian species of Marasmius have been compiled from data on macro- and micromorphological features of basidiomata, supplemented with data on geographical and ecological distribution. Details on nomenclature and …

Cloning And Sequencing Of The Eco Rii Monomer Fragment Of Satellite Dna From The Mouse Mus Musculus, Timothy Patrick Boyle

Masters Theses

Total mouse satellite DNA was isolated in cesium chloride gradients in the presence of the dye Hoechst 33258. This satellite fraction was digested with Bst Nl, an isoschizomer of the restriction enzyme Eco RII, and the 234 base pair monomer fragment was cloned into the plasmid vector pBS. Dideoxynucleotide sequencing of the cloned DNA yielded significant homology with the published consensus sequence of mouse satellite DNA (92%).

Effect Of Photoperiod On Developmental Morphology And Enolase Isoenzyme Immunohistochemistry In Rat And Djungarian Hamster Superficial Pineal Glands, Chalmer D. Mcclure

Loma Linda University Electronic Theses, Dissertations & Projects

The best understood functional activity of the pineal gland is its diurnal production of melatonin in response to environmental lighting cues. Several enzymes of the melatonin pathway respond to daily photoperiod changes, for example hydroxyindole-O-methyltransferase (HIOMT) and serotonin N-acetyltransferase (SNAT). Increased levels of the glycolytic enzyme neuron-specific enolase (NSE) are thought to reflect increased physiological demands placed on neurons and neuroendocrine tissues. Homodimer non-neuronal enolase isoenzyme (NNE) is immunolocalized to cells, and the hybrid enolase (consisting of subunits from NSE and NNE) has been seen in cerebellar stellate and basket cells. Although not rate limiting, concentrations of both NSE and …

The Effect Of An Anti-Osteoclastic Agent And A Collagen Sponge On Root Resorption Following Regenerative Periodontal Surgery In The Dog, Rudolf Hahn

Loma Linda University Electronic Theses, Dissertations & Projects

Previous studies indicate that root resorption is a frequent sequela to significant amounts of periodontal repair following experimental regenerative periodontal surgical procedures in animal models. To study if root resorption can be reduced or prevented, periodontal repair was evaluated in large horizontal circumferential defects in the dog in the absence of influence from the oral environment. Diphosphonate (Cl₂ MDP) applied topically to the root surfaces prior to wound closure as well as the use of a collagen sponge membrane (Helistat®) draped over the root surfaces and a control with no additional treatment after defect creation were the modalities employed. …

Immunosuppressive Activity Derived From The Uc729-6 Lymphoblastoid And M21-Hpb Melanoma Cell Lines, Charlene J. Repique

Loma Linda University Electronic Theses, Dissertations & Projects

Previous work conducted in our laboratories, using a murine model, suggested that soluble factors secreted by tumor cells suppress lymphocyte responses. To apply this premise to human tumors, we studied the effects of UC729-6 (lymphoblastoid B-cell) and M21-HPB (malignant melanoma) conditioned media (CM) on lymphocyte proliferation assays, as well as on the growth of other cells. The CM was collected at 2-5 day intervals from cultures of UC729-6 and M21- HPB cells in serum-free media. In assays using phytohemagglutinin (PHA) and concanavalin A (ConA), mononuclear peripheral blood cells from healthy human donors showed decreased [³H]-thymidine ([³H]-Tdr) …

The Effects Of Varying Light Exposure Times On The Colonization And Sporulation Of Gigaspora Margarita In The California Pepper, Mary E. Kirker

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Arts and Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Mary E. Kirker on July 24, 1989.

Microwell Based Competitive Enzyme Immunoassay For Quantifying Serum Triiodothyronine And Thyroxine Levels, Yiwen Yang

Chemistry & Biochemistry Theses & Dissertations

A microwell based competitive enzyme immunoassay for determination of total thyroxine and triiodothyronine in human serum was developed with use of horseradish peroxidase conjugate. The assay, which can be performed in only 1.5 hours at room temperature, involves simultaneous incubation of T₃- or T₄- HRPO conjugate and serum sample in anti-T₃ or anti-T₄ gamma globulin fraction coated polystyrene microwells.

Isolation of anti-T₃ and anti-T₄ gamma globulin fractions were accomplished from whole antiserum by using DEAE-cellulose chromatograph. A carbodiimide coupling method was described to prepare the T₄- and T₃-HRPO …

The Temporal And Spatial Distribution Of Blue Crab Larvae On The Mid-Atlantic Bight Adjacent To The Chesapeake Bay, 1982-1983, Lyle Michael Varnell

OES Theses and Dissertations

Recruitment processes are important information for the management of commercially and recreationally exploited marine species. Recruitment processes of the Chesapeake Bay's most important commercial and recreational species, the blue crab, are unknown, due in part to the lack of knowledge of the processes directly preceeding recruitment.

The present study was undertaken to investigate and explain the movement and developmental patterns of blue crab larvae while offshore. Interannual and intraseasonal variations of these patterns were also examined.

Sampling occurred from May to November during 1982 and 1983. Larvae were collected at three depths (neuston layer (upper 10 cm), one meter and …

Picoplankton/Phytoplankton Dynamics In Hampton Roads, Virginia, Leslie E. Dorworth

OES Theses and Dissertations

The biomass distribution of the three main size components of the phytoplankton (net, nano, and picoplankton) was examined in the lower Chesapeake Bay. Work was done at two stations during the spring and neap tidal cycles when freshwater flow into the bay was at its seasonal minimum and maximum. Emphasis was placed on how the picoplankton ( < 2.0 μm, > 0.2 μm) reacted during the sampling period.

Picoplankton made up approximately 13% of the total biomass, during the observed time period, at the more oceanic station. The picoplankton fraction contributed up to 16% of the total biomass at the estuarine station. From this …

Spectroscopic Study Of Decomposition Products Of Gossypol, David Bernard Hankins

Chemistry & Biochemistry Theses & Dissertations

Basic research into reproductive physiology has begun to identify possible new approaches to controlling fertility. Gossypol, a natural polyphenol compound isolated from cottonseed oil, has been shown to be an effective male antifertility agent. The decomposition products of gossypol which are thought to possess more potent antifertility effects than the parent compound gossypol have been studied extensively in more recent research. The purpose of this research is to develop techniques and methods which could be used to identify decomposition products of gossypol. A model decomposition reaction by which a smaller molecule can be isolated in a pure state has been …

Simultaneous Determination Of 3-Methoxy-4-Hydroxyphenyl-Glycol, Homovanillic Acid, And 5-Hydroxyindoleacetic Acid In Plasma By High Performance Liquid Chromatography, Yung-Te Tseng

Chemistry & Biochemistry Theses & Dissertations

A rapid, reliable, and sensitive method for simultaneous determination of 3-methoxy-4-hydroxyphenylglycol (MHPG), Homovanillic acid (HVA), and 5-hydroxyindoleacetic acid (5- HIAA) in plasma is described. The method is based on high performance liquid chromatography with electrochemical detection. Samples were filtered with Amicon Centrifree micropartition systems and extracted with organic solvent before chromatography. Chromatographic separation of sample components was achieved isocratically in a C18 reverse-phase column. Quantitation was performed by measuring the peak height ratios of the sample components to an internal standard. Analytical recoveries ranged from 50.78 (SD 4.2%) to 67.94 (SD 3.28). Within-run coefficient of variations (CVs) ranged from 3.78 …

Mounting Sex Pheromone: A Novel Pheromone Responsible For Mate Recognition In The Ixodidae, Jgc Hamilton

Theses and Dissertations in Biomedical Sciences

Cholesterol oleate was demonstrated to be the cuticular contact sex pheromone of Dermacentor variabilis. This pheromone which has been termed the Mounting Sex Pheromone (MSP) was also demonstrated to be present on the surface of D. andersoni, Amblyomma maculatum and A. americanum. This contact sex pheromone enables males excited and attracted by 2,6-dichlorophenol to identify the female as a potential mating partner. The MSP is the second in the series of three sex pheromones guiding the hierarchy of behavioral responses which constitute tick courtship behavior. Tests with D. variabilis and D. andersoni showed that this pheromone …

The Remipedia (Crustacea): A Study Of Their Reproduction And Ecology, Jill Yager

Biological Sciences Theses & Dissertations

Remipedes are an unusual group of troglobitic crustaceans that live exclusively in anchialine caves. Since their discovery in 1979, nine species have been described, seven of which are found in caves in the West Indies, one from the Yucatan peninsula of Mexico and one from the Canary Islands. Most of what is known about these animals has come mainly from taxonomic descriptions. Little has been published about their reproductive biology or ecology. The objectives of this dissertation were to investigate the reproductive biology and ecology of the remipedes inhabiting Sagittarius Cave on Grand Bahama Island, Bahamas.

Sagittarius Cave was chosen …

Modulation Of Macrophage Chemiluminescence By Biological Response Modifiers And Neuroendocrine Hormones, Jeffrey M. Tosk

Loma Linda University Electronic Theses, Dissertations & Projects

Neuroendocrine mediators have been shown to influence a broad spectrum of immune functions suggesting the existence of a neuroimmune regulatory axis effecting feedback control of the immune response. Most studies in support of this paradigm have employed either intact animals or immune cells derived from blood and other tissues. Under these conditions it becomes difficult to cope with numerous variables associated with the complex milieu of the immune system and the interpretation of data. An in vitro model using a murine macrophage cell line, J774, was developed to circumvent these problems. Using this model, macrophages activated by exposure to the …

Design Specifications And Requirements Of Paper For Flexographic Printing, Martha L. Stankey

Paper Engineering Senior Theses

Flexographic printing is a direct printing from the plate to the paper. This process has been growing during the last few years, especially in the newspaper area. One of the problems in the industry today, however, is the lack of good paper specifications for the flexographic printing process. The objective of this project was to gather samples of good flexographic newsprint, and to test them for paper properties in order to determine a set of paper specifications for flexographic newsprint. Also, lithographic (the predominant printing process in the newspaper industry) newsprint samples were gathered in order to compare between the …

A Comparison Of Maximal And Submaximal Heart Rate And Oxygen Uptake In Combined Arm And Leg Bicycling And Rowing, Victoria L. Asaro

Masters Theses

No abstract provided.