Pharmacy and Pharmaceutical Sciences Commons^™

Isolation And Partial Characterization Of Bioactive Fucoxanthin From Himanthalia Elongata Brown Seaweed: A Tlc-Based Approach, Gaurav Rajauria, Nissreen Abu-Ghannam

Articles

Seaweeds are important sources of carotenoids, and numerous studies have shown the beneficial effects of these pigments on human health. In the present study, Himanthalia elongata brown seaweed was extracted with a mixture of low polarity solvents, and the crude extract was separated using analytical thin-layer chromatography (TLC).The separated compounds were tested for their potential antioxidant capacity and antimicrobial activity against Listeria monocytogenes bacteria using TLC bioautography approach. For bio-autography, the coloured band on TLC chromatogram was visualized after spraying with DPPH and triphenyltetrazolium chloride reagents which screen antioxidant and antimicrobial compounds, respectively, and only one active compound was ...

In-House Mentorship, Reza Karimi

Faculty Scholarship (PHRM)

No abstract provided.

Perception, Expression And Management Of The Pain Of Childbirth, Mary H. Christenson

Senior Honors Papers

In order to be a competent nurse on an American labor and delivery unit it is important to have an understanding of the pain of childbirth. This includes a thorough understanding of pain as a sensation and its manifestation during the birthing experience. An understanding of pain is useless, however, unless standardized pain assessment practices are used. The most accurate pain assessment is associated with a general understanding of cultural trends in pain perception and expression. Along with culture, other factors also influence how a woman senses and copes with the pain of labor. Anxiety is one of these influential ...

Oral Contraceptives, Stephenie G. Fortier

Senior Honors Papers

Cyclic oral contraceptive and continuous oral contraceptives have many similarities yet a few distinct differences. One of the major differences between cyclic and continuous oral contraceptives is that cyclic oral contraceptive pills have twenty one active pills and seven inactive pills versus the new continuous oral contraceptive pills which have 28 active pills and no inactive pills. However, though cyclic and continuous oral contraceptives do have a few differences, both types of oral contraceptives affect a woman’s body in similar ways. When choosing between cyclic and continuous oral contraceptives, it is important for women to be informed about the ...

Towards Elucidation Of A Viral Dna Packaging Motor, Chad T. Schwartz

Theses and Dissertations--Pharmacy

Previously, gp16, the ATPase protein of phi29 DNA packaging motor, was an enigma due to its tendency to form multiple oligomeric states. Recently we employed new methodologies to decipher both its stoichiometry and also the mechanism in which the protein functions to hydrolyze ATP and provide the driving force for DNA packaging. The oligomeric states were determined by biochemical and biophysical approaches. Contrary to many reported intriguing models of viral DNA packaging, it was found that phi29 DNA packaging motor permits the translocation of DNA unidirectionally and driven cooperatively by three rings of defined shape. The mechanism for the generation ...

Acute Vibration Induces Transient Expression Of Anabolic Genes In The Murine Intervertebral Disc, Matthew R. Mccann, Priya Patel, Kim L. Beaucage, Yizhi Xiao, Corey Bacher, Walter L. Siqueira, David W. Holdsworth, S. Jeffrey Dixon, Cheryle A. Séguin

Physiology and Pharmacology Publications

No abstract provided.

Patients' Perceptions Of Community Pharmacists Giving Vaccinations, Darlinghton O. Njoku, Vineeta Rao, Morgan R. Bailey, Matt Madden, Joshua A. Sitler, Tracy R. Frame

The Research and Scholarship Symposium

No abstract provided.

Stemming The Global Trade In Falsified And Substandard Medicines, Lawrence O. Gostin, Gillian J. Buckley, Patrick W. Kelley

Georgetown Law Faculty Publications and Other Works

Drug safety and quality is an essential assumption of clinical medicine, but there is growing concern that this assumption is not always correct. Poor manufacturing and deliberate fraud occasionally compromises the drug supply in the United States, and the problem is far more common and serious in low- and middle-income countries with weak drug regulatory systems. An Institute of Medicine consensus committee report identified the causes and possible solutions to the problem of falsified and substandard drugs around the world.

The vocabulary people use to discuss the problem is itself a concern. The word counterfeit is often used innocuously to ...

Emergency Contraceptive (Ec) Use In Indigent Populations, Ashley Benjamin, Kasandra Chambers, Melissa Mcnicol, Amy Roy, Kurtis Schultz, April Yoakam, Miriam A. Ansong, Pharm.D., Tracy R. Frame, Ph.D.

The Research and Scholarship Symposium

No abstract provided.

A Case Study On Pharmacy To Explore The Perceptions Of Pharmacy Leaders And Policy Makers On The Benefits, Risks, And Alternatives Of The Doctorate As The Entry Level Degree In Health Professions, Heidi Marie Crocker

Dissertations

The clinical doctorate is an emergent trend in many health profession disciplines. Collier (2008) projects continued momentum toward higher degrees for entry into practice and advancing the field in health professions. There has been minimal research on how the trend of doctoral education in health professions will affect health professions education, delivery of services, and interdisciplinary relationship among health care providers, or the wider society (Freburger, King, & Slifkin, 2008). This research focused on the transition to the clinical doctorate in one profession, Pharmacy, retrospectively examining the inception and enactment phase of the Pharm.D. The study provides important insight into ...

Impact Of A Pharmacist-Managed Smoking Cessation Program, Alexandra Archambault, Belinda Darkwah, Kale Hanavan, Ellery Kent, Myriam Shaw Ojeda, Larisa Yuchimiuk, Kelly J. Hiteshew, Pharm.D.

The Research and Scholarship Symposium

No abstract provided.

Patient Satisfaction With Pharmacist Counseling, Kristina Burban, Samuel Franklin, Sarah Marks, Trevor Reed, Brandon Spears, Aleda M. H. Chen, Ph.D.

The Research and Scholarship Symposium

No abstract provided.

Genetic Manipulation Of Leishmania Parasites Facilitates The Exploration Of The Polyamine Biosynthetic Pathway As A Potential Therapeutic Target, Sigrid C. Roberts

Faculty Scholarship (PHRM)

Parasites of the genus Leishmania cause a variety of devastating and often fatal diseases, ranging from cutaneous ulcerative lesions to fatal visceralizing infections that affect an estimated 12 million people worldwide. Unfortunately, vaccines are not available and the cturent arsenal of drugs used to treat leishmaniasis is far from ideal. Thus the need for new therapeutic targets and a better understanding of host-parasite interactions is urgent. One biochemical pathway that has been successfully exploited for the treatment of a related parasitic disease, African trypanosomiasis, is the polyamine biosynthetic pathway. In order to elucidate the polyamine biosynthetic pathway and to explore ...

Embedded Assessment And Evidence-Based Curriculum Mapping: The Promise Of Learning Analytics, Jane M. Souza

Pharmacy Faculty Publications

At Wegmans School of Pharmacy, we have adopted an embedded assessment approach to curriculum mapping and data collection on student learning outcomes achievement. In essence, we capture the data from the course level exams that our faculty members craft to measure student learning.

Novel Neurotrophic Growth Factor Signaling Pathway Mediates Changes In Epigenetics Through The Redox And Methylation Influence Of Eaat3-Mediated Cysteine Uptake, Nathaniel Wilkens Hodgson

Pharmaceutical Science Dissertations

The level of oxidative stress increases with age, and abnormal elevations are a hallmark of many neurological and neuropsychiatric disorders, including Alzheimer's disease. In neurons, the glutamate, aspartate, and cysteine transporter EAAT3 is the predominant transporter of cysteine, the rate-limiting precursor for synthesis of glutathione, the primary intracellular antioxidant. Thus, the regulation of EAAT3 is crucial in maintaining redox balance in neurons, and agents that change its activity can exert important effects on neuronal redox status. Furthermore, since methylation is highly sensitive to redox status, these agents can also modulate methylation status, including DNA methylation, yielding epigenetic consequences.

The ...

Investigating Key Post-Pks Enzymes From Gilvocarcin Biosynthetic Pathway, Nidhi Tibrewal

Theses and Dissertations--Pharmacy

Gilvocarcin V (GV) belongs to the angucycline class of antibiotics that possesses remarkable anticancer and antibacterial activities with low toxicity. Gilvocarcin exhibits its light induced anticancer activity by mediating crosslinking between DNA and histone H3. When photo-activated by near-UV light, the C8 vinyl group forms a [2+2] cycloadduct with thymine residues of double stranded DNA. _D-fucofuranose is considered essential for histone H3 interactions. However, the poor water solubility has rendered it difficult to develop gilvocarcin as a drug. We aim to design novel gilvocarcin analogues with improved pharmaceutical properties through chemo-enzymatic synthesis and mutasynthesis. Previous studies have characterized ...

Multi-Compartmental Delivery Systems For Peptide And Dna Vaccines In Melanoma Immunotherapy, Mayurkumar Chimanlal Kalariya

Pharmaceutical Science Dissertations

The vaccine delivery systems containing adjuvants modulate magnitude, breadth, quality, and longevity of immune responses to antigen vaccinations aimed to induce cell-mediated immunity. For vaccines targeting tumor antigens, adjuvant-based delivery systems are necessary to overcome various tolerance mechanisms and facilitate induction of cytotoxic T lymphocytes (CTLs) that can traffic to and lyse malignant cells. The objective of this doctoral thesis project was to develop a multi-compartmental vaccine delivery system for safe and efficient delivery of a variety of melanoma antigens for immunotherapy.

Water-in-oil-in-water (W/O/W) multiple emulsions-based multi-compartmental vaccine delivery systems containing the gp100 peptide or hgp100 plasmid DNA ...

The Mechanism Of Monoacylglycerol Lipase (Mgl) Inactivation: A Study Using Nuclear Magnetic Resonance Spectroscopy And Mass Spectrometry, Ioannis L. Karageorgos

Pharmaceutical Science Dissertations

The endocannabionoid signaling system is composed of two main cannabinoid receptors (CB1 and CB2 ), their principal endogenous ligands (anandamide (AEA) and 2-arachidonoylglycerol (2-AG)) and enzymes primarily responsible for degrading endocanabinoids such as fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MGL), abhydrolase domain-containing protein-6 and N-acylethanolamine-hydrolyzing acid amidase (NAAA) that are responsible for reduction of endocanabinoid system activity and its consequent (patho) physiological effects. Although FAAH may inactivate 2-AG in vitro, its main in vivo biological substrate is anandamide, whereas MGL exclusively hydrolyzes 2-AG to stoichiometric amounts of arachidonic acid (AA) and glycerol in vivo. Unlike anandamide, that is a partial ...

Cysteine Dependence Of Insulin-Like Growth Factor Effects On Redox And Methylation Status, Yiting Li

Pharmaceutical Science Master's Theses

Methionine synthase serves as a bridge between the methionine cycle and the dopamine-stimulated phospholipid methylation cycle. Cobalamin is the co-factor of methionine synthase to catalyze the conversion of homocysteine to methionine. Oxidized cobalamin halts methionine synthase activity, increasing homocysteine diversion to the transsulfuration pathway leading to formation of cysteine which can then be converted to the primary intracellular antioxidant glutathione.

Methionine synthase is a multi-domain enzyme and its linker domain termed `cap' is responsible for protecting cobalamin from dissociation and oxidation. In human brain, the cap domain of methionine synthase can be alternatively spliced in response to oxidative stress. Spliced ...

Enhancement Of In Vitro Therapeutic Efficacy Using Combination Paclitaxel And Ceramide Therapy Using Egfr Targeted Biodegradable Polymeric Nanoparticles In Ovarian Cancer Model, Darshna Rajendrakumar Patel

Pharmaceutical Science Master's Theses

Currently, tumor-cell resistance to the chemotherapy is key reason for failure of the clinical treatment of cancer cells; almost all chemotherapeutic strategies used are susceptible to multidrug resistance (MDR). The development of MDR is major hindrance to eradication of cancer as it interferes with the effect of therapeutic agents on tumors and increases chances of cancer resurgence.

MDR refers to a cross-resistance to structurally and functionally unrelated drugs thereby rendering the tumor unresponsive to most chemotherapeutic options. It results from microenvironmental selection, like poor delivery efficiency of drug at the disease site, phenotypic alterations in cancer cells leading to enhanced ...