A V-To-F Substitution In Sk2 Channels Causes Ca2+ Hypersensitivity And Improves Locomotion In A C. Elegans Als Model, Young-Woo Nam, Sabia N. Baskoylu, Dimitris Gazgalis, Razan Orfali, Meng Cui, Anne C. Hart, Miao Zhang
Pharmacy Faculty Articles and Research
Small-conductance Ca2+-activated K+ (SK) channels mediate medium afterhyperpolarization in the neurons and play a key role in the regulation of neuronal excitability. SK channels are potential drug targets for ataxia and Amyotrophic Lateral Sclerosis (ALS). SK channels are activated exclusively by the Ca2+-bound calmodulin. Previously, we identified an intrinsically disordered fragment that is essential for the mechanical coupling between Ca2+/calmodulin binding and channel opening. Here, we report that substitution of a valine to phenylalanine (V407F) in the intrinsically disordered fragment caused a ~6 fold increase in the Ca2+ sensitivity of SK2-a channels. This substitution resulted in a ...
Extraction And Purification Of (E)-Resveratrol From The Bark Of Maine's Native Spruces, 2018 University of Maine
Extraction And Purification Of (E)-Resveratrol From The Bark Of Maine's Native Spruces, Panduka S. Piyaratne
Electronic Theses and Dissertations
Resveratrol is a plant secondary metabolite which protects plants from pathogenic invasions and acts as a remedial agent against injury, stress, and tissue damage by UV radiation. With its characteristic stilbene structure, resveratrol adopts (E) and (Z) isomeric forms. However, the (E) isomer is the most biologically active and abundant form of resveratrol in nature.
(E)- Resveratrol confers a variety of benefits to human health and well-being. Besides its antioxidant activity, the role of (E)-resveratrol as an anti-inflammatory, anti-aging, cardioprotection, and anticancer agent has been a widely explored research interest. Many plant species including mulberry, peanut, and grape are ...
Comparing The Side Effects Of Prescription Opioids And Medicinal Marijuana In The Treatment Of Chronic Pain, 2018 Carroll College - Helena
Comparing The Side Effects Of Prescription Opioids And Medicinal Marijuana In The Treatment Of Chronic Pain, Callie Glenn, Ryan Bopp
Carroll College Student Undergraduate Research Festival
Opioids are medications that interact with receptors in the central nervous system to provide pain relief, and have been a standard foundation for treatment of chronic pain since their discovery. But advancements in medical technology have led to the discovery of several alternatives to narcotics, one being medical marijuana. Cannabis has become increasingly pertinent in the medical field due to the medicinal and recreational legalization of the substance in several states. The purpose of this Evidence Based Practice Brief is to determine how the side effects of medical marijuana compared to those of opioid treatment affect quality of life for ...
Nutraceuticals: An Alternative Treatment For Influenza Virus, 2018 Brigham Young University
Nutraceuticals: An Alternative Treatment For Influenza Virus, Siale Teaupa
FHSS Mentored Research Conference
Influenza virus is a contagious respiratory pathogen that infects hundreds of thousands of people a year, making it a serious global health concern. The virus has a rapid mutation rate and has developed resistance to current antiviral agents, making it a difficult target for effective treatment. There is an increasing need to identify new treatments for Influenza. Recently, we have tested nutraceuticals as an effective alternative for blocking Influenza. We have tested Jamaican Sorrel, Black Currant Berries, and Manuka Honey paired with Bee Pollen for antiviral activity. We have demonstrated that these nutraceuticals block the 2009 Pandemic California Influenza strain ...
Discovery Of Natural Product Analogs Against Ethanol-Induced Cytotoxicity In Hippocampal Slice Cultures, 2018 University of Kentucky
Discovery Of Natural Product Analogs Against Ethanol-Induced Cytotoxicity In Hippocampal Slice Cultures, Meredith A. Saunders-Mattingly
Theses and Dissertations--Psychology
An estimated 13.9% of Americans currently meet criteria for an alcohol (ethanol; EtOH) use disorder (AUD). While there are 4 medications approved by the Food and Drug Administration (FDA) to treat AUD, these treatments have demonstrated poor clinical efficacy. Our ongoing research program encompasses a multi-tiered screening of a natural product library and validation process to provide novel information about the mechanisms underlying EtOH-induced changes in neurobiology and to identify novel chemical scaffolds to be exploited in the development of pharmacological treatments for AUD in a rodent organotypic hippocampal slice culture model. Initial screens of several natural product compounds ...
Progress Toward The Development Of A "Catch/Release" Strategy For Isolating Salvinorin A, From Plant Materails Including Microwave Promotion, Douglas Armstrong, Logan Smith
Faculty Scholarship – Chemistry
We are investigating using the Diels-Alder and retro-Diels-Alder reactions as a potential “catch/release” strategy for isolating Salvinorin A from plant samples (Salvia divinorum), hopefully to improve the current method, which is very long and laborious. Salvinorin A has analgesic activity by activating the kappa opioid receptor, not the mu receptor, and thus it has potential for being developed into a non-addicting analgesic.
Applied Drug Development And Combinatorial Strategies For Antimicrobial Treatment, 2017 Texas A & M University - College Station
Applied Drug Development And Combinatorial Strategies For Antimicrobial Treatment, Steven K. Lai Hing
Andrews Research Conference
Streptococcus mutans JH1140 is a strain of bacteria which produces a lantibiotic product, named mutacin 1140. Mutacin 1140 has been shown to be effective at inhibiting Gram-positive bacterial infections caused by Staphylococcus aureus and Streptococcus pneumoniae. Mutacin 1140 is a ribosomally synthesized peptide antibiotic that undergoes extensive posttranslational modifications (PTM). We have found that Mutacin 1140 and an aminoglycoside, Kanamycin, when combined together, act synergistically against Staphylococcus aureus. This was determined by performing serial kill curve dilution overlays on solid media, followed up with kill curve by microdilution plate, and most recently confirmed with kill curve CFU count plates on ...
Discovery Of Natural Product-Based Antimycobacterial Agents Effective Against Non-Replicating Bacilli, 2017 University of Tennessee Health Science Center
Discovery Of Natural Product-Based Antimycobacterial Agents Effective Against Non-Replicating Bacilli, Shajila Siricilla
Theses and Dissertations (ETD)
New antimycobacterial molecules that kill non-replicating Mycobacterium tuberculosis (Mtb) were identified by screening libraries of synthetic natural products. De novo screening of a 400-membered library of aurachin RE analogs resulted in discovery of UT-317 ((R)-20). UT-317 is a selective vitamin K2 biosynthesis (MenA) inhibitor that killed replicating and non-replicating Mtb at 2.31 μg/mL (MIC) and 0.85 μg/mL, respectively. A 50-membered library of capuramycin analogs was evaluated in their enzymatic inhibitory activities against translocase I (MraY/MurX) and prenyl-phosphate-GlcNAc-1-phosphate transferase (WecA). UT-01320 (45) is identified as a selective WecA inhibitor that kills both replicating and non-replicating ...
The Pharmacognosy And Therapeutic Efficacy Of Turmeric (Curcuma Longa): A Systematic Review, 2017 University of Rhode Island
The Pharmacognosy And Therapeutic Efficacy Of Turmeric (Curcuma Longa): A Systematic Review, Maghan Ballantyne
Senior Honors Projects
Turmeric is a well-known natural product, native to Southeast Asia, commonly
used for a variety of cultural traditions and health benefits. Generally referred
to as curcumin, it is a member of the Zingiberaceae, or ginger family, utilized
for its roots and known for its vibrant yellow hue. Culturally, it is primarily
incorporated into cooking and as an integral part of religious rituals and
ceremonies. Chemically, turmeric is classified as a phenolic compound, made
up of many curcuminoids, each with varying levels of activity. Therapeutically,
its health benefits include anti-inflammatory, anti-hyperlipidemia, anti-oxidant
and anti-pruritic properties. The pharmacognosy of curcumin modulates
Crispr: The Last Piece Of The Genetic Puzzle, 2017 Butler University
Crispr: The Last Piece Of The Genetic Puzzle, Brad Broyles
CRISPR/Cas9 is an emerging gene editing tool. This technology, which evolved naturally as a defense mechanism for bacteria and other prokaryotes, has been repurposed to edit genes in a wide array of cells, including human cells. This paper outlines the discovery, mechanism, and potential applications of CRISPR/Cas9.
Adverse Reactions Of Biological Therapies In Patients With Psoriasis, 2017 Carol Davila University, Department of Dermatology
Adverse Reactions Of Biological Therapies In Patients With Psoriasis, Maria I. Sârbu, Mircea Tampa, Mădălina I. Mitran, Cristina I. Mitran, Alexandra M. Limbău, Simona R. Georgescu
Journal of Mind and Medical Sciences
Psoriasis is a chronic, immune-mediated disorder characterized by well demarcated, erythematous plaques covered by thick, silvery-white scales, most often located on the knees, elbows, sacral area and scalp. It has a significant impact on the patient's quality of life.
Biological therapies revolutionized the treatment of psoriasis vulgaris but there has been concern regarding the use of those agents due to severe adverse reactions reported in patients receiving TNF-α inhibitors for various inflammatory diseases.
The aim of this paper is to review the most important adverse reactions reported in patients receiving biological treatments. The most common and severe side effects ...
Pharmacological Basis For The Medicinal Use Of Polyherbal Formulation And Its Ingredients In Cardiovascular Disorders Using Rodents, A Malik, Malik Hassan Mehmood, H Channa, Ms Akhtar, Ah Gilani
Department of Biological & Biomedical Sciences
A compound herbal formulation (POL4) has been used in the indigenous system of medicine to treat cardiometabolic disorders like diabetes and associated hypertension. POL4 and most of its constituents have not been studied widely for its therapeutic use in hypertension. This study is aimed to determine the efficacy and possible insight into mechanism(s) for the medicinal use of POL4 and its ingredients in hypertension.
Ethical Imperatives Of Timely Access To Orphan Drugs: Is Possible To Reconcile Economic Incentives And Patients’ Health Needs?, 2017 University of Massachusetts Amherst
Ethical Imperatives Of Timely Access To Orphan Drugs: Is Possible To Reconcile Economic Incentives And Patients’ Health Needs?, Rosa Rodriguez-Monguio, T. Spargo, Enrique Seoane-Vazquez
Pharmacy Faculty Articles and Research
More than 6,800 rare diseases and conditions have been identified in the US, which affect 25–30 million Americans. In 1983, the US Congress enacted the Orphan Drug Act (ODA) to encourage the development and marketing of drugs to treat rare diseases and conditions. This study analyzed all orphan designations and FDA approvals since 1983 through 2015, discussed the effectiveness of incentives for the development of treatments for rare diseases, and reflected on the ethical imperatives for timely access to orphan drugs.
Study data were derived from the Food and Drug Administration (FDA) Orange Book and the ...
The International Scientific Association For Probiotics And Prebiotics (Isapp) Consensus Statement On The Definition And Scope Of Prebiotics, Glenn R. Gibson, Robert W. Hutkins, Mary Ellen Sanders, Susan L. Prescott, Raylene A. Reimer, Seppo J. Salminen, Karen Scott, Catherine Stanton, Kelly S. Swanson, Patrice D. Cani, Kristin Verbeke, Gregor Reid
Faculty Publications in Food Science and Technology
In December 2016, a panel of experts in microbiology, nutrition and clinical research was convened by the International Scientific Association for Probiotics and Prebiotics to review the definition and scope of prebiotics. Consistent with the original embodiment of prebiotics, but aware of the latest scientific and clinical developments, the panel updated the definition of a prebiotic: a substrate that is selectively utilized by host microorganisms conferring a health benefit. This definition expands the concept of prebiotics to possibly include non-carbohydrate substances, applications to body sites other than the gastrointestinal tract, and diverse categories other than food. The requirement for selective ...
Infusion Of Essentail Oils In Agarose Gels To Create Antimicrobial Surfaces, 2017 Honors College, Pace University
Infusion Of Essentail Oils In Agarose Gels To Create Antimicrobial Surfaces, Simran Guron
Honors College Theses
The current use of antibiotics is very excessive in multiple settings, from treating patients to being present in cleaning products. This overuse of antibiotics is resulting in multiple consequences, ranging from having a negative impact on human health to breeding antibiotic-resistant strains of microorganisms. Antimicrobial surfaces are useful today to aid in sterilization, especially in the medical setting. This would help prevent the spread of infection of microorganisms in these settings. In our research, agarose acts as the antimicrobial surface and essential oils would act as the solution incorporated into the surface that has the antimicrobial properties. Agarose is a ...
Association Of Various Concentrations Of Cat's Claw Herb (Uncaria Tomentosa) On Lymphocyte Proliferation And Nitric Oxide Expression: An In-Vitro Study Of Osteoarthritis, 2017 University of Northern Iowa
Association Of Various Concentrations Of Cat's Claw Herb (Uncaria Tomentosa) On Lymphocyte Proliferation And Nitric Oxide Expression: An In-Vitro Study Of Osteoarthritis, Noha Fadlalddin
Electronic Theses and Dissertations
Uncaria tomentosa, also known as cat’s claw, is an indigenous herb which originates from the Amazon River basin and has proven to be highly effective via case report and limited epidemiological evaluation in the pallative treatment of a number of inflammatory conditions. The herb is particularly preferred on the American market due to its various uses for healing, which provides the impetus for this research. The herb is being used to treat osteoarthritis and claims have been made for its ability to: enhance the repair of deoxyribonucleic acid (DNA), support joint health and improve immune function, enable normal division ...
The Effects Of Adaptogens On The Physical And Psychological Symptoms Of Chronic Stress, 2017 Georgia State University
The Effects Of Adaptogens On The Physical And Psychological Symptoms Of Chronic Stress, Tosin O. Ajala
DISCOVERY: Georgia State Honors College Undergraduate Research Journal
Stress is a state of disharmony or threatened homeostasis. The maintenance of homeostasis in stages of internal or external challenges, called stressors, requires constant adjustments of hormonal, behavioral, and autonomic functions. The nuances of life may bring about unwanted stress to the human body. Some of the top causes of stress in America include work, finances, relationships, and health. There are numerous physical and psychological symptoms associated with long-term “chronic” stress, which include chronic fatigue, frustration, irritability, insomnia, frequent headaches, chest and back pain, weakness, and weight gain or weight loss. These symptoms of chronic stress are associated with depression ...
Evaluation Of Accurate Dietary Supplement Product Labeling, 2016 University of New England
Evaluation Of Accurate Dietary Supplement Product Labeling, Laura Hitchcock, Brandon Kong, Hoang Pham, John Redwanski
Pharmacy Practice Faculty Posters
Herbal supplement sales in the U.S. increased by 7.5% between 2014 and 2015, from $6.441 billion to $6.922 billion, according to newly released data from the Nutrition Business Journal (NBJ). Dietary supplements and over-the-counter medications (OTC’s) do not have to be proven safe or effective before being sold to consumers like prescription medications. Under the Dietary Supplement Health and Education Act of 1994, manufacturers only have to prove their product causes no harm to consumers. Dietary supplement labels are not reliable, since they are not regulated. Without verification a consumer cannot be sure that what ...
Vitamin K2 (Menaquinone) Supplementation And Its Benefits In Cardiovascular Disease, Osteoporosis, And Cancer, 2016 Marshall University, Joan C. Edwards School of Medicine
Vitamin K2 (Menaquinone) Supplementation And Its Benefits In Cardiovascular Disease, Osteoporosis, And Cancer, Grant S. Buchanan, Md, Thomas Melvin, Brandon Merritt, Charles Bishop, Md, Franklin D. Shuler, Md, Phd
Franklin D. Shuler
Vitamin K is known to play an essential role in the coagulation cascade; however, a growing body of research has found that a subtype of this vitamin, vitamin K2 (menaquinone) may have a beneficial effect in osteoporosis, cardiovascular disease, and cancer. This purpose of this article is to provide a comprehensive review of recent literature regarding menaquinone and its role in human health. This review discusses the physiology of menaquinone, its clinical benefits in cardiovascular disease, osteoporosis, and cancer, and how it may interact with certain medications. The authors conclude that menaquinone supplementation has been shown to improve carboxylation of ...
Countercurrent Chromatography Fractions Of Plant Extracts With Anti-Tuberculosis Activity, 2016 Olivet Nazarene University
Countercurrent Chromatography Fractions Of Plant Extracts With Anti-Tuberculosis Activity, Douglas Armstrong, Nathan C. Krause, Drew Frey, J. Brent Friesen, Baojie Wan, Jordan Gunn, Scott Franzblau
Faculty Scholarship – Chemistry
Samples of numerous plant species were received from the southwestern part of the USA, from Richard Spjut, and plant samples were collected here in Illinois. All were extracted with typical solvents, giving crude residues, some of which were subjected to chromatographic methods. Some of the crude residues and some of the fractions were tested for anti-tuberculosis activity and/or antibacterial activity.
In a general way, bioactive natural products are dealt with very well by Liang & Fang. More specifically, the southwestern part of the United States has a large variety of indigenous plants many of which have not been investigated for ...