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Bioanalytical Determination Of Glucose Concentration In Sports Drinks Using Uv/Vis Spectroscopy, Laney Hedgeman, Brianna Bond 2021 Kennesaw State University

Bioanalytical Determination Of Glucose Concentration In Sports Drinks Using Uv/Vis Spectroscopy, Laney Hedgeman, Brianna Bond

Symposium of Student Scholars

Abstract

The purpose of our project is to indirectly monitor the enzymatic activity of glucose oxidase as it reacts with the glucose present in sports drinks through the quantitative analysis of the generated ferricyanide. As glucose oxidase catalyzes the oxidation of beta-D-glucose in the presence of oxygen, D-glucono-1,5-lactone is produced along with hydrogen peroxide. The hydrogen peroxide is used in a subsequent reaction with ferrocyanide catalyzed by horse radish peroxidase to produce water and the chromophore ferricyanide. This compound absorbs in the ultraviolet/visible spectrum at 420 nm, which can be quickly measured using Ultraviolet/Visible Spectroscopy. Since the molar ratio of …


High Protein Rice Flour In The Development Of Gluten Free Pasta, Ana Isabel Serrano Marana 2021 Louisiana State University

High Protein Rice Flour In The Development Of Gluten Free Pasta, Ana Isabel Serrano Marana

LSU Master's Theses

Cahokia Rice is a biofortified high protein (10-12%) variety of rice. This rice has been evaluated and studied to create gluten-free products like cakes, muffins, and bread. This study was aimed to develop and analyze a gluten-free pasta using this high protein rice. Different pasta made with high-protein-white-rice-flour (HPWRP), high-protein-brown-rice flour (HPBRP), commercial-white-rice flour (CWRP), commercial-brown-rice flour (CBRP), and wheat-pasta (WP) as control were prepared and dried.

The cooking quality of the pasta was evaluated by measuring pasting properties, cooking time, water absorption capacity and cooking loss. Physical properties such as color and texture (firmness, compression energy, and work of …


Techniques To Improve The Volume, Texture And Nutritional Quality Of Gluten Free Bread, Zachary Christman 2021 University of Nebraska - Lincoln

Techniques To Improve The Volume, Texture And Nutritional Quality Of Gluten Free Bread, Zachary Christman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This article highlights the use of soy flour to improve the protein and fiber of gluten free bread. Also, the use of an extrusion process on soybean and corn flour blends improves the volume and texture of the bread. A full listing of the ingredients and method for production of higher quality gluten free bread is included.


Bringing Biochemistry Home: Transforming Milk Into Yogurt, Ashley King 2021 University of Mississippi

Bringing Biochemistry Home: Transforming Milk Into Yogurt, Ashley King

Honors Theses

Communicating the beauty and complexity of biochemistry to students in a large classroom during the pandemic: what a challenge! We undertook a novel endeavor in the Department of Chemistry and Biochemistry by introducing a mandatory kitchen chemistry experiment in a lecture course. Milk, the epitome of our identity as mammals, also contains all of the major biochemical macromolecules studied in Biochemistry I. Further, the making of yogurt invokes physical processes that are the major processes and molecular forces that dominate the content of the course. Here, we report the results of massive parallel experiment conducted in the kitchens of the …


Has Maize Overtaken Our Reality? A Personal Briefing, Biochemical Comparison, Agrigenomics, And History Of Maize, Nader Pahlevan 2021 University of Mississippi

Has Maize Overtaken Our Reality? A Personal Briefing, Biochemical Comparison, Agrigenomics, And History Of Maize, Nader Pahlevan

Honors Theses

Maize (Zea mays ssp. Mays) is a revolutionary cereal grain that has raced to the world’s most popular staple crop, transforming societies and impacting history. This paper aims to build and portray the story maize has created through its journey to world domination. The important details that encompass this literature are maize’s cultural significance in my life’s story, the comparison of various starches broken down into amylose and amylopectin ratios, a summative historical account on maize’s spread throughout numerous parts of the old world, and the genetical analysis of maize that explains the key features that have led …


Color And Structural Modifications Of Alkaline Extracted Sunflower Protein Concentrates And Isolates Using L-Cysteine And Glutathione, Akira Ishii 2021 Chapman University

Color And Structural Modifications Of Alkaline Extracted Sunflower Protein Concentrates And Isolates Using L-Cysteine And Glutathione, Akira Ishii

Food Science (MS) Theses

Sunflower protein can be extracted at alkaline pH with sulfites or organic solvent de-phenolization of sunflower seed meals if greening is unwanted. This greening is promoted at alkaline pH when chlorogenic acid (CGA) oxidizes and reacts with free amine groups from amino acids, such as lysine. Thiol-containing dough conditioners: L-cysteine hydrochloride and reduced glutathione (GSH) were investigated as an alternative de-greening strategy to de-phenolization. Varying pH from 7 to 11 and concentration of 0.82 to 5.6 mM were randomly assigned by Response Surface Methodology (RSM) to evaluate the combined and additive greening inhibition effect of thiol and extraction pH. The …


Influence Of Encapsulation Of Supplemental Amino Acids On Their Utilization In Broilers, Ethan A. Collins 2021 University of Arkansas, Fayetteville

Influence Of Encapsulation Of Supplemental Amino Acids On Their Utilization In Broilers, Ethan A. Collins

Poultry Science Undergraduate Honors Theses

This study was developed to assess whether microencapsulation of amino acids (AA) improves their absorption to increase broiler growth performance and reduce nitrogen excretion compared to standard feed-grade AA. Five hundred and sixty Ross 708 male broilers were randomly distributed to 70 battery cages and reared for 21 days. Reported data is grouped into periods: 0-7 days, 0-14 days, and 0-21 days. A total of 5 treatment diets were fed: an industry-based control and 4 diets with decreased levels of methionine, lysine, and threonine in either encapsulated or free forms, with or without a botanical feed additive. Encapsulated AA increased …


Gut Community Response To Wheat Bran And Pinto Bean, ShuEn Leow 2021 University of Nebraska-Lincoln

Gut Community Response To Wheat Bran And Pinto Bean, Shuen Leow

Department of Food Science and Technology: Dissertations, Theses, and Student Research

There is general consensus among the scientific community that dietary fibers reduce the risk of Western diseases through their fermentation by beneficial microbial communities in the human gut. However, dietary fibers in wheat bran (WB) and pinto bean (PB) are incompletely fermented by the gut microbiota. Therefore, there is a critical need to identify gut microbial communities that can increase fermentation of dietary fibers from these foods to maximize their disease-preventing properties. The goal of this study was to identify such communities with increased capacity to ferment the dietary fibers in WB and PB. To accomplish these goals, a stepwise …


Food Sensitivity In Individuals With Altered And Unaltered Digestive Tracts, Walker Carson 2021 University of Nebraska-Lincoln

Food Sensitivity In Individuals With Altered And Unaltered Digestive Tracts, Walker Carson

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Immunoglobulin G (IgG) against food proteins has become the subject of much discussion with regards to its role in adverse food reactions. An estimated 20% of the population suffers from some type of food intolerance. Food sensitivity can present with a vast range of symptoms and severities. Diet can have a substantial impact on the wellbeing of individuals with diseases of the gastrointestinal tract, such as Crohn’s disease or ulcerative colitis. Incidentally, these diseases have been associated with elevated levels of food-specific IgG as well an increased likelihood of food sensitivity. The presence of food-specific IgG and food sensitivity in …


Novel Sources Of Food Allergens, Lee Palmer 2021 University of Nebraska-Lincoln

Novel Sources Of Food Allergens, Lee Palmer

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Technological advancement and globalization have led to the spread of foods to countries where the food does not yet have a documented history of consumption, in other words, novel foods. Novel foods also encompass truly novel foods, foods that have been processed in a novel manner, and novel means of exposure. With novel foods comes the potential of food allergens that pose an uncharacterized risk to those with food allergies. Food allergies are an increasingly important facet of public health. Therefore, a deeper understanding of novel foods as well as methods to evaluate consumers’ potential risk is necessary. Literature reviews …


Combatting Inefficient Food Waste In Nwa With Food Loops, Davis Ehlers 2021 University of Arkansas, Fayetteville

Combatting Inefficient Food Waste In Nwa With Food Loops, Davis Ehlers

Economics Undergraduate Honors Theses

It is no longer a speculation that the actions of the human race have had detrimental impacts on the environment. As consumers become increasingly aware of this fact- and the fact that many of the issues are caused by bad business and environmental management, many individuals are wondering what they can do about it. This growing area of demand is where many businesses have begun to take on the challenge of being profitable while benefiting the environment at the same time, and some have even found ways to create a profit by engaging in activities that directly benefit the environment. …


Corruption In Capsules: How It Is Legal For Companies To Put Harmful Ingredients In Vitamins And Dietary Supplements, Emily Leggiero 2021 Kutztown University of Pennsylvania

Corruption In Capsules: How It Is Legal For Companies To Put Harmful Ingredients In Vitamins And Dietary Supplements, Emily Leggiero

English Department: Research for Change - Wicked Problems in Our World

The vitamin and supplement industry has increased exponentially in profits as well as potential products on the market since the turn of the century. However, these products are not regulated, nor do they undergo any premarket clinical research or testing. Public health is compromised by vitamins and supplements that are available for American consumption that is disproportionately unregulated to their chemically similar counterparts. This wicked problem is facilitated through the combination of historical legislative definitions that has since been distorted for corrupt administrative gain through the allotment of corporate expenditures. Company disbursements are made to the same policymakers that create …


Bibliometric Analysis Of The 200 Most Cited Articles On Antioxidant From 1976-2020, Ghouse Modin Nabeesab Mamdapur Mr., Neelesh Kumar Nema Dr., Baby Kumaranthara Chacko Dr., Jose Paul Dr. 2021 Synthite Industries Pvt. Ltd., Kolenchery, Ernakulam, Kerala, INDIA

Bibliometric Analysis Of The 200 Most Cited Articles On Antioxidant From 1976-2020, Ghouse Modin Nabeesab Mamdapur Mr., Neelesh Kumar Nema Dr., Baby Kumaranthara Chacko Dr., Jose Paul Dr.

Library Philosophy and Practice (e-journal)

Antioxidant helps to reduce various disorders and control pathological conditions such as cancer, aging, cardiovascular disease, cataracts, immune system decline, and brain dysfunction etc. In this study, the top 200 highly cited articles (citations 327,657) with total 647 authors were retrieved from Elsevier’s Scopus database (105,440 hits) by the keyword “antioxidant” published across the worldwide for the timespan 1976-2020 years. The data were analysed through bibliometric indicators by using VOSviewer and biblioshiny tools to explore various parameters i.e. keywords co-occurrence, authors contribution, highly cited journals, affiliations/organization engaged and worldwide collaboration. In the year 2001 and 1996, maximum number of …


Interactions Between Titanium Dioxide Nanoparticles (Nps) And Mucin, . Boimin 2021 University of Massachusetts Amherst

Interactions Between Titanium Dioxide Nanoparticles (Nps) And Mucin, . Boimin

Doctoral Dissertations

Titanium dioxide nanoparticles (TiO2 NPs) are widely used in many food, consumer, and industrial products. However, little is known about the overall effects of TiO2 NPs on the environment or human health. In order to elucidate the fate, transformation, transport, and toxicological impact of TiO2 NPs, a better understanding is needed of how the physicochemical properties of TiO2 NPs (e.g. size, charge, curvature, hydrophobicity, and surface functionality) interact with their microenvironments (e.g. pH, temperature, bile acids, microbiome, enzymes, surface-active components, and biopolymers). Living organisms including humans have a natural mechanism to protect themselves from physical, biological, …


Development Of Pudding And Gravy With Rice Starch, Stearic Acid And Lysine, Andrea Suazo Rosales 2021 Louisiana State University

Development Of Pudding And Gravy With Rice Starch, Stearic Acid And Lysine, Andrea Suazo Rosales

LSU Master's Theses

Starch is a common ingredient used in the food industry for many products. This study was focused on developing products (gravy and pudding) with a clean label starch. The clean label starch was prepared with rice starch, 1% stearic acid, and 6% lysine, which was gelatinized, freeze-dried, and grinded. Both products were made with three different starch types: rice starch, commercial modified starch, and clean label starch. These starches were analyzed with DSC, SEM, Megazyme Amylose/Amylopectin kit, and RVA. With the results from the RVA, SEM, and DCS it is inferred that the freeze-drying process has altered the structure of …


Postharvest Salicylic Acid And Melatonin Dipping Delay Ripening And Improve Quality Of ‘Sensation’ Mangoes, Mohamed A. Awad, Adel D. Al-Qurashi 2021 King Abdulaziz University, Jeddah, Saudi Arabia

Postharvest Salicylic Acid And Melatonin Dipping Delay Ripening And Improve Quality Of ‘Sensation’ Mangoes, Mohamed A. Awad, Adel D. Al-Qurashi

The Philippine Agricultural Scientist

Freshly harvested mature-green ‘Sensation’ mangoes were dipped for 10 min in 2 mM salicylic acid (SA) and 0.2 mM melatonin (MT) alone or in combination and stored at 23±1°C and 60–70%RH. SA or MT delayed ripening measured as higher green peel color (lower a* values), firmness, titratable acidity (TA) and membrane stability index (MSI) and lower weight loss, total soluble solids (TSS), TSS/TA ratio and hydrolytic enzyme activities (polygalacturonase, xylanase and α-amylase) compared to that of untreated fruit. Total phenolic content (TPC) in peel of treated fruit increased with fluctuations during ripening and was higher than the control. In pulp, …


Evaluation Of The Inactivation Kinetics Of Cronobacter Sakazakii In Infant Formula Treated By Radio Frequency Dielectric Heating And Uvc Light [Research Note], Yun Deng, Yuwei Wu, Yuanrong Zheng, Danfeng Wang, Zhenmin Liu 2021 Shanghai Jiaotong University

Evaluation Of The Inactivation Kinetics Of Cronobacter Sakazakii In Infant Formula Treated By Radio Frequency Dielectric Heating And Uvc Light [Research Note], Yun Deng, Yuwei Wu, Yuanrong Zheng, Danfeng Wang, Zhenmin Liu

The Philippine Agricultural Scientist

We studied the inactivation kinetics of Cronobacter sakazakii (CS) in infant formula subjected to radio frequency dielectric heating (RF) alone or sequential RF-UVC irradiation for 30 min. We used one linear model and two nonlinear models to fit the data and compared the results. Our findings revealed that CS was more susceptible to the combined treatment than to RF alone at the same temperature and that inactivation rate increased with increasing temperature. The sequential RF (90°C, 5 min) - UVC (25 min) treatment was the most effective at inactivating CS. The survival curves of CS were non-linear. …


Postharvest Fruit Quality Of Apple Influenced By Ethylene Antagonist Fumigation And Ozonized Cold Storage, Vijay Y. Tokala, Zora Singh, Poe N. Kyaw 2021 Edith Cowan University

Postharvest Fruit Quality Of Apple Influenced By Ethylene Antagonist Fumigation And Ozonized Cold Storage, Vijay Y. Tokala, Zora Singh, Poe N. Kyaw

Research outputs 2014 to 2021

The effects of two new ethylene antagonists namely 1H-cyclopropabenzene (BC) and 1H-cyclopropa[b]naphthalene (NC), as well as 1-methylcyclopropene (1-MCP) on ethylene production and fruit quality of Cripps Pink and Granny Smith apple in ozonized cold storage, were investigated. When compared to control, Cripps Pink fruit fumigated with BC and NC exhibited significantly lowest ethylene production and respiration, whilst the Granny Smith fruit treated with 1-MCP exhibited lowest ethylene production followed by NC and BC treatments. Application of ozone in cold storage maintained higher levels of sugars but elevated ethylene production in both the apple cultivars. No significant interaction was recorded between …


Novel Uplc-Ms/Ms Method For Standardization Of Niazimicin Content In Edible Seeds And Leaves Of Moringa Oleifera Lam., Eman M. Abdelsayed, Amira Abdel Motaal, Rasha S. Hanafi 2021 University of Hertfordshire hosted by Global Academic Foundation, Egypt

Novel Uplc-Ms/Ms Method For Standardization Of Niazimicin Content In Edible Seeds And Leaves Of Moringa Oleifera Lam., Eman M. Abdelsayed, Amira Abdel Motaal, Rasha S. Hanafi

Journal of Food and Drug Analysis

Moringa oleifera (MO) is a highly nutritious plant, whose leaves and seed pods are consumed in Africa, Asia, sub-Himalayan regions and South America. A novel ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for the standardization of the bioactive thiocarbamate compound from MO, niazimicin (NZ) in seeds and leaves, is developed, optimized and validated according to the International Conference on Harmonization (ICH) guidelines, using desipramine as the internal standard. Multiple reaction monitoring detection of transitions 358.05>106.86 and 266.38 > 193.04 with collision energy of 25V and 40V, respectively were used. A gradient was optimized at 35-55%B …


Three-Way Analysis-Based Ph-Uv-Vis Spectroscopy For Quantifying Allura Red In An Energy Drink And Determining Colorant's Pka, Erdal Dinç Prof., Nazangül Ünal, Zehra Ceren Ertekin 2021 Ankara University

Three-Way Analysis-Based Ph-Uv-Vis Spectroscopy For Quantifying Allura Red In An Energy Drink And Determining Colorant's Pka, Erdal Dinç Prof., Nazangül Ünal, Zehra Ceren Ertekin

Journal of Food and Drug Analysis

Three-way analysis-based pH-UV-Vis spectroscopy was proposed for quantifying allura red in an energy drink product without the need for chromatographic analysis, and determining the colorant’s pKa without using any titration technique. In this study, UV-Vis spectroscopic data matrices were obtained from absorbance measurements at five different pH levels from pH 8 to pH 12 and arranged as a three-way array (wavelength x sample x pH). In the three-way analysis procedure, parallel factor analysis (PARAFAC) was implemented to decompose the three-way array into a set of trilinear components. Each set of three components relates to spectral, pH and relative concentration profiles …


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