Medicine and Health Sciences Commons^™

Experimental Model Of Atrophic Rhinitis In Gnotobiotic Pigs, Mark R. Ackermann, R. B. Rimler, J. R. Thurston

Mark R. Ackermann

To study the pathogenesis of atrophic rhinitis, gnotobiotic pigs (n = 6) were inoculated intranasally with a sterile sonicate of a toxigenic strain of Bordetella bronchiseptica (0.16 mg of protein per ml) at 5 days of age, and they were then inoculated intranasally with 1 ml (5,250 CFU/ml) of a live, toxigenic strain of Pasteurella multocida at 7 days of age. Pigs were necropsied at 2, 5, 9, 14, 21, and 28 days postinoculation; those pigs necropsied after 5 days had developed turbinate atrophy. Other gnotobiotic pigs received the following inoculation protocols: (i) a sterile sonicate of a nontoxigenic strain …

Interphotoreceptor Retinoid-Binding Protein (Irbp) In Progressive Rod-Cone Degeneration (Prcd)—Biochemical, Immunocytochemical And Immunologic Studies, Gustavo Aguirre, Barbara Wiggert, Geetha Kutty, Kenneth Long, Lila Inouye, Igal Gery, Gerald Chader

Gustavo D. Aguirre, VMD, PhD

Colonization Of The Pharyngeal Tonsil And Respiratory Tract Of The Gnotobiotic Pig By A Toxigenic Strain Of Pasteurella Multocida Type D, Mark R. Ackermann, N. F. Cheville, J. E. Gallagher

Mark R. Ackermann

Seven-day-old gnotobiotic pigs were inoculated intranasally with Pasteurella multocida and euthanatized 2, 5, 9, and 14 days after inoculation. Tissues from the oropharynx and respiratory tract of pigs were cultured quantitatively and analyzed microscopically. Pigs remained afebrile and alert, except one that died of acute fibrinopurulent pneumonia. Pasteurella multocida was isolated in greatest numbers from the pharyngeal tonsils, but only in low numbers from turbinate, trachea, lung, spleen, and liver. Significant histologic changes were limited to the tonsil. Infected pigs developed mild tonsillitis with lymphocytic hyperplasia, and accumulation of cell debris and bacteria in crypts. Capsular antigens of P. multocida, …

The Cone Matrix Sheath In The Normal And Diseased Retina: Cytochemical And Biochemical Studies Of Peanut Agglutinin-Binding Proteins In Cone And Rod-Cone Degeneration, Kenneth Long, Gustavo Aguirre

Gustavo D. Aguirre, VMD, PhD

Glycosaminoglycan And Collagen Metabolism In Arylsulfatase B-Deficient Retinal Pigment Epithelium In Vitro, Gustavo D. Aguirre, Lawrence E. Stramm, W Li, M Haskins

Gustavo D. Aguirre, VMD, PhD

Lectin Histochemistry Of Trachea And Lung Of Healthy Turkeys (Meleagris Gallopavo) And Turkeys With Pneumonia, Mark R. Ackermann, N. F. Cheville, P. G. Detilleux

Mark R. Ackermann

Thirteen lectins were used to characterize lectin-binding specificity of glycoconjugates on sections of formalin-fixed lung and trachea from seven normal turkeys, two turkeys with acute pneumonia, and two turkeys with chronic pneumonia. Neuraminidase was used to digest sialic acid residues. One N-acetylgalactosamine-binding lectin and two N-acetylgalactosamine/galactose-binding lectins stained the apical membrane and cytoplasm of multifocal cells that lined air atria and hyperplastic granular cells. Other lectins in these groups stained ciliated cells of the trachea and bronchi and air capillary epithelial cells. Sialic acid residues were on apical surfaces of ciliated and nonciliated tracheal and bronchial lining cells, air capillary …

Immunity Induced In Rats Vaccinated With Toxoid Prepared From Heat-Labile Toxin Produced By Pasteurella Multocida Serogroup D, J. R. Thurston, R. B. Rimler, Mark R. Ackermann, N. F. Cheville, J. M. Sacks

Mark R. Ackermann

Rats were vaccinated with a toxoid (D-toxoid) prepared from purified heat-labile toxin (D-toxin) produced by Pasteurella multocida serogroup D. Vaccination of rats with D-toxoid prevented death and other effects of D-toxin (hepatic necrosis, development of elevated leukocyte counts, lymphopenia, neutrophilia, and elevated complement titers) that occurred in phosphate buffered saline (PBS)-vaccinated control rats.

Β-Glucuronidase Mediated Pathway Essential For Retinal Pigment Epithelial Degradation Of Glycosaminoglycans. Disease Expression And In Vitro Disease Correction Using Retroviral Mediated Cdna Transfer, Lawrence Stramm, John Wolfe, Edward Schuchman, Mark Haskins, Donald Patterson, Gustavo Aguirre

Gustavo D. Aguirre, VMD, PhD

Segregation Distortion In Inheritance Of Progressive Rod Cone Degeneration (Prcd) In Miniature Poodle Dogs, Gregory M. Acland, Susan Halloran-Blanton, Joann A. Boughman, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

Evaluation Of Chicken Heterophil Adherence, Claire B. Andreasen, Kenneth S. Latimer, W. L. Steffens

Claire B. Andreasen

Adherence of chicken heterophils was evaluated at 37 C using preconstructed columns containing various weights of nylon fiber (75 mg, 100 mg, or 125 mg) and whole blood anticoagulated with sodium heparin or 10% disodium ethylenediamine tetraacetic acid (EDTA). Additionally, 50-mg and 75-mg nylon fiber columns incubated at 41 C were used to evaluate heterophil adherence at an increased temperature. The mean percent adherence for heparin-anticoagulated blood applied to 75-mg, 100-mg, and 125-mg nylon fiber columns at 37 C was 76%, 92% and 97.4%, respectively. Samples applied to 50-mg and 75-mg columns at 41 C had adherence values of 27% …

Organ Hypertrophy And Responses Of Colon Microbial Populations Of Growing Swine To High Dietary Protein, James S. Dickson, Wilson G. Pond, Vincent H. Varel, Friday O. I. Anugwa, Gary S. Ross

James S. Dickson

Thirty-two castrated male crossbred growing pigs (average initial wt 26.9 kg) were used to determine the effect of a high level of dietary protein (37%) compared with a normal level of protein (15%) on enterobacteria and Campylobactersp. inhabitation in the large intestine and on visceral organ hypertrophy and the interrelationships between these two factors. Pigs were kept in pairs (eight pens of two pigs/diet) and fed their respective diets ad libitum. Eight pigs (two pens of two pigs fed each diet) were killed at wk 4, 8. 12 and 16 without fasting. Fecal samples were obtained every 2 wk from …

Metabolic Labeling Of Rod Outer Segment Phospholipids In Miniature Poodles With Progressive Rod-Cone Degeneration (Prcd), Mary Wetzel, Christian Fahlman, Paul O'Brien, Gustavo Aguirre

Gustavo D. Aguirre, VMD, PhD

Frequency Of The Codon 807 Mutation In The Cgmp Phosphodiesterase Β-Subunit Gene In Irish Setters And Other Dog Breeds With Hereditary Retinal Degeneration, Gustavo D. Aguirre, V Baldwin, K M. Weeks, Gregory M. Acland, Kunal Ray

Gustavo D. Aguirre, VMD, PhD

Retinal Pigment Epithelial Glycosaminoglycan Metabolism: Intracellular Versus Extracellular Pathways. In Vitro Studies In Normal And Diseased Cells, Gustavo D. Aguirre, Lawrence E. Stramm, M Haskins

Gustavo D. Aguirre, VMD, PhD

Separation Of Turkey Heterophils From Blood Using Two-Step Ficoll-Hypaque Discontinuous Gradients, Kenneth S. Latimer, Ingrid M. Kircher, Claire B. Andreasen

Claire B. Andreasen

A method is presented to separate turkey heterophils from anticoagulated whole blood using two-step Ficoll-Hypaque discontinuous gradients and ammonium chloride lysis of contaminating erythrocytes. Heterophils can be isolated from multiple blood samples within 3 to 4 hours. Using this technique, 66.4 +- 18.4% (mean +- standard deviation) of blood heterophils were harvested. Final cell isolates averaged 96.0 +- 2.9% heterophils with few contaminating eosinophils (2.5 +- 2.3%) or basophils (1.6 +- 1.8%). Cell viability, as determined by trypan blue dye exclusion, was 98.0 +- 1.4%.

Determination Of Chicken And Turkey Plasma And Serum Protein Concentrations By Refractometry And The Biuret Method, Claire B. Andreasen, Kenneth S. Latimer, Ingrid M. Kircher, John Brown

Claire B. Andreasen

Plasma and serum protein concentrations were determined in chickens and turkeys by refractometry (with human and veterinary refractometers)and by the biuret method. Chicken and turkey serum protein values were significantly lower than respective plasma protein values according to both methods. Refractometer readings for both plasma and serum correlated closely with the results of the biuret test (r2 = 0.72 to 0.97). These findings indicate that plasma and serum protein values may be determined accurately in chickens and turkeys with a handheld refractometer.

Separation Of Avian Heterophils From Blood Using Ficoll-Hypaque Discontinuous Gradients, Claire B. Andreasen, Kenneth S. Latimer

Claire B. Andreasen

Rapid separation of avian heterophils from anticoagulated whole blood was achieved using Ficoll-Hypaque discontinuous gradients. An average of 14.4% of blood heterophils was harvested with a mean purity exceeding 99%. Heterophil viability, as determined by trypan blue dye exclusion, averaged 99.8%. The integrity of isolated heterophils was evaluated by cytochemical staining and ultrastructural examination. Cytochemical staining reactions of heterophils in whole blood and of isolated cell suspensions were similar. No ultrastructural abnormalities were observed. Using this procedure, viable intact heterophils were rapidly isolated from blood with an acceptable cell yield and purity for cell function studies.

S-Antigen In A Hereditary Visual Cell Disease. Immunocytochemical And Immunological Studies, Gustavo D. Aguirre, K Long, N Philp, I Gery

Gustavo D. Aguirre, VMD, PhD

Acute Fibrinopurulent Blepharitis And Conjunctivitis Associated With Staphylococcus Hyicus, Escherichia Coli, And Streptococcus Sp. In Chickens And Turkeys, N. F. Cheville, J. Tappe, Mark R. Ackermann, A. Jensen

Mark R. Ackermann

Multiple outbreaks of acute severe fibrinopurulent lesions of the eyelids occurred in chickens and turkeys. Lesions began as tiny foci of epidermal necrosis and ulceration and spread to involve the entire eyelid. Scabs overlying the epidermis contained large gram-positive cocci; lesser numbers of small cocci and gram-negative bacilli were in more superficial areas. Staphylococcus hyicus was isolated from birds in all stages of the disease. Escherichia coli and Streptococcus sp. were isolated only during severe stages; no anaerobic bacterial pathogens were isolated. Vasculitis and perivascular lymphocytic infiltrates in deep layers of the dermis suggested that a staphylococcal toxin may have …

Bovine Ileal Dome Lymphoepithelial Cells: Endocytosis And Transport Of Brucella Abortus Strain 19, Mark R. Ackermann, N. F. Cheville, B. L. Deyoe

Mark R. Ackermann

Ligated ileal loops of calves were inoculated with Brucella abortus and examined at 2, 4, 6, 10, and 24 hours post-inoculation. B. abortus was identified by light and electron microscopy using immunoperoxidase and antibody-coated colloidal gold techniques. B. abortus was detected in vesicles, phagolysosomes, and large vacuoles of lymphoepithelial cells. Numbers of intracellular bacteria decreased with time after inoculation. B. abortus was also seen between and below lymphoepithelial cells and free in the dome interstitium and intestinal lymph vessels. Neutrophils and macrophages in both epithelium and lamina propria contained intact or degraded bacteria within phagosomes, phagolysosomes, and multivesicular bodies. These …

Arylsulfatase B Activity In Cultured Retinal Pigment Epithelium: Regional Studies In Feline Mucopolysaccharidosis Vi., Lawrence E. Stramm, R J. Desnick, M Haskins, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

A Tribute To Charles W. Raker, Robert R. Marshak

Charles W. Raker (1920 - 2014)

A tribute to Charles W. Raker on the occasion of his retirement from the University of Pennsylvania School of Veterinary Medicine in 1985 by his colleague Robert R. Marshak.

Reductions In Taurine Secondary To Photoreceptor Loss In Irish Setters With Rod-Cone Dysplasia, Gustavo D. Aguirre, S Y. Schmidt

Gustavo D. Aguirre, VMD, PhD

Disease Expression In Cultured Pigment Epithelium. Feline Mucopolysaccharidosis Vi., Lawrence E. Stramm, M Haskins, R J. Desnick, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

Experimental Eye Research Volume 35, Issue 6, December 1982, Pages 625–642 Cover Image Retinal Degenerations In The Dog Iii Abnormal Cyclic Nucleotide Metabolism In Rod-Cone Dysplasia, Gustavo Aguirre, Debora Farber, Richard Lolley, Paul O'Brien, James Alligood, R Fletcher

Gustavo D. Aguirre, VMD, PhD

Pathogenesis Of Progressive Rod-Cone Degeneration In Miniature Poodles, Gustavo D. Aguirre, James P. Alligood, P O'Brien, N Buyukmihci

Gustavo D. Aguirre, VMD, PhD

Pigmentary Abnormalities Of The Macula In Rhesus Monkeys: Clinical Observations, R W. Bellhorn, C D. King, Gustavo D. Aguirre, H Ripps, I M. Siegel, H C. Tsai

Gustavo D. Aguirre, VMD, PhD

Gyrate Atrophy Of The Choroid And Retina In A Cat, D L. Valle, A P. Boison, P Jezyk, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

Congenital Stationary Night Blindness: An Animal Model, D A. Witzel, R D. Wilson, E L. Smith, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

The Electroretinogram In Dogs With Inherited Cone Degeneration, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD