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- Atrophic rhinitis (3)
- Swine (3)
- Lectins (2)
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- Avian speecies (1)
- BRSV (1)
- Backscatter imaging (1)
- Bordetella (1)
- Bovine leukocyte adhesion deficiency (1)
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- CD18 (1)
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- Cattle (1)
- Chondroitinase (1)
- EBM11 (1)
- Endocytosis (1)
- FJ2 integrins (1)
- Granular cells (1)
- Immunocytochemistry (1)
- Inflammation (1)
- Integrins (1)
- Intravascular macrophages (1)
- Leukocyte adhesion deficiency (1)
- Multinucleate spermatocytes (1)
- Mycobacterium paratuberculosis (1)
Articles 1 - 30 of 30
Full-Text Articles in Medicine and Health Sciences
Enhanced Adhesion Of Pasteurella Multocida To Cultured Turkey Peripheral Blood Monocytes, Ingrid M. Pruimbroom, Richard B. Rimler, Mark R. Ackermann
Enhanced Adhesion Of Pasteurella Multocida To Cultured Turkey Peripheral Blood Monocytes, Ingrid M. Pruimbroom, Richard B. Rimler, Mark R. Ackermann
Mark R. Ackermann
Capsular hyaluronic acid (HA) mediates adhesion of serogroup A strains of Pasteurella multocida to elicited turkey air sac macrophages (TASM). In contrast, freshly isolated turkey peripheral blood monocytes (TPBM) do not bind serogroup A strains. Following culture of TPBM for 6 days in chamber slides, adhesion of the bacteria to TPBM increased gradually. Incubation in chamber slides coated with entactin-collagen IV-laminin (ECL) attachment matrix or exposure to phorbol myristate acetate (PMA) further enhanced the adhesion ofP. multocida to TPBM. Addition of HA, but not Arg-Gly-Asp peptide, to TPBM culture inhibited bacterial adherence similarly to the inhibition previously reported for TASM. …
A Highly Adherent Phenotype Associated With Virulent Bvg+-Phase Swine Isolates Of Bordetella Bronchiseptica Grown Under Modulating Conditions, Karen B. Register, Mark R. Ackermann
A Highly Adherent Phenotype Associated With Virulent Bvg+-Phase Swine Isolates Of Bordetella Bronchiseptica Grown Under Modulating Conditions, Karen B. Register, Mark R. Ackermann
Mark R. Ackermann
The ability of Bvg(-)-phase and Bvg(+)-phase Bordetella bronchiseptica swine isolates, grown under modulating or nonmodulating conditions, to adhere to swine ciliated nasal epithelial cells was determined. When virulent strains were cultivated at 37 degrees C in the Bvg+ phase, numerous adherent bacteria (approximately eight per cell, depending on the strain used) were observed. However, when such strains were grown under modulating conditions (23 degrees C), a significant increase in the level of attachment was seen, suggesting that B. bronchiseptica produces a Bvg-repressed adhesin under these conditions. bvg mutant strains, including an isogenic bvgS mutant, adhered minimally. Western blots indicated that …
Effects Of Pasteurella Multocida Toxin On Porcine Bone Marrow Cell Differentiation Into Osteoclasts And Osteoblasts, S. M. Gwaltney, R. J. S. Galvin, K. B. Register, R. B. Rimler, Mark R. Ackermann
Effects Of Pasteurella Multocida Toxin On Porcine Bone Marrow Cell Differentiation Into Osteoclasts And Osteoblasts, S. M. Gwaltney, R. J. S. Galvin, K. B. Register, R. B. Rimler, Mark R. Ackermann
Mark R. Ackermann
The effect of Pasteurella multocida toxin (PMT) on porcine osteoclast and osteoblast differentiation was studied using in vitro cell culture systems. When grown in the presence of Vitamin D3, isolated porcine bone marrow cells formed multinucleated cells with features characteristic of osteoclasts. Exposure of bone marrow cells to Vitamin D3 and PMT during growth resulted in formation of increased numbers and earlier appearance of osteoclasts compared to controls. Ultrafiltered medium from PMT-treated cells likewise increased osteoclast numbers, suggesting that a soluble mediator may be involved in the action of PMT. When cell cultures were treated with fluorescein-labeled PMT, fluorescence was …
A Porcine Model For The Evaluation Of Virulence Of Bordetella Bronchiseptica, Mark R. Ackermann, K. B. Register, C. Gentry-Weeks, S. M. Gwaltney, T. Magyar
A Porcine Model For The Evaluation Of Virulence Of Bordetella Bronchiseptica, Mark R. Ackermann, K. B. Register, C. Gentry-Weeks, S. M. Gwaltney, T. Magyar
Mark R. Ackermann
Studies of virulence factors of Bordetella bronchiseptica require a suitable system. Such a system was devised in colostrum-deprived, caesarean-derived pigs, aged 7 d. In two different experiments, pigs (n=11) were inoculated intranasally with 106 colony-forming units of the virulent strain 4609. In the same way, further pigs (n=11) were inoculated with a strain (B133) of unknown virulence. No significant differences between 4609 and B133 colonization were seen. However, colonization of the turbinates was significantly higher than that of the trachea, lung and tonsil, and a significantly higher degree of colonization was present at 11 d post-inoculation (PI) than at 15 …
Passage Of Cd18- And Cd18+ Bovine Neutrophils Into Pulmonary Alveoli During Acute Pasteurella Haemolytica Pneumonia, Mark R. Ackermann, M. E. Kehrli Jr., K. A. Brogden
Passage Of Cd18- And Cd18+ Bovine Neutrophils Into Pulmonary Alveoli During Acute Pasteurella Haemolytica Pneumonia, Mark R. Ackermann, M. E. Kehrli Jr., K. A. Brogden
Mark R. Ackermann
CD18 is a subunit for three β2 integrin molecules (Mac-1, p150, 95, LFA-1), which are expressed on the plasma membrane of neutrophils. These molecules mediate passage of neutrophils into sites of infection. In children and animals that lack CD18 expression, neutrophil infiltration is impaired in most tissues. However, in lung, CD18-neutrophils have been identified in the airway spaces during spontaneous episodes of pneumonia. To determine whether CD18 is vital for passage through the pulmonary alveolar wall, lung lobes of cattle with neutrophils that were deficient in CD18 expression (CD18-) and cattle with normal CD18 expression (CD18+) were inoculated with Pasteurella …
Capsular Hyaluronic Acid-Mediated Adhesion Of Pasteurella Multocida To Turkey Air Sac Macrophages, Ingrid M. Pruimboom, Richard B. Rimler, Mark R. Ackermann, Kim A. Brogden
Capsular Hyaluronic Acid-Mediated Adhesion Of Pasteurella Multocida To Turkey Air Sac Macrophages, Ingrid M. Pruimboom, Richard B. Rimler, Mark R. Ackermann, Kim A. Brogden
Mark R. Ackermann
Serogroup A strains of Pasteurella multocida, the major cause of fowl cholera, are resistant to phagocytosis in nonimmunized birds. Adherence studies with a capsulated strain of P multocida (serotype A:3) and turkey air sac macrophages in culture showed that the bacteria were capable of adhering in large numbers to the macrophages but were not internalized. A noncapsulated variant of the bacteria (serotype -:3) showed little or no adherence and was not internalized. These data indicated that the adhesive properties were caused by the presence of a capsule on the bacteria. The role of capsular hyaluronic acid in adherence to macrophages …
Comparison Of Polyclonal Antibodies To Three Different Preparations Of Mycobacterium Paratuberculosis In Immunohistochemical Diagnosis Of Johne's Disease In Cattle, Judith R. Stabel, Mark R. Ackermann, Jesse P. Goff
Comparison Of Polyclonal Antibodies To Three Different Preparations Of Mycobacterium Paratuberculosis In Immunohistochemical Diagnosis Of Johne's Disease In Cattle, Judith R. Stabel, Mark R. Ackermann, Jesse P. Goff
Mark R. Ackermann
Polyclonal antisera were raised in rabbits against preparations of live and heat-killed Mycobac-terium paratuberculosis and cell-wall proteins of M. paratuberculosis and were evaluated as diagnostic tools in immunohistochemical staining of bovine tissue. Live preparations of M. paratuberculosis (LMp) were inoculated intraperitoneally or intravenously at 109/ml. Heat-killed M. paratuberculosis (HKMp) was prepared by treatment of bacteria at 85 C for 10 minutes. Cell-wall proteins were isolated from M. paratuberculosis and conjugated to keyhole limpet hemocyanin to improve antigenicity (KLH-CWPMp). The HKMp and KLH-CWPMp preparations were emulsified in incomplete Freund's adjuvant before subcutaneous inoculation of rabbits. Antibody titers in the terminal blood …
Porcine Reproductive And Respiratory Syndrome Virus Enters Cells Through A Low Ph-Dependent Endocytic Pathway, Luiz C. Kreutz, Mark R. Ackermann
Porcine Reproductive And Respiratory Syndrome Virus Enters Cells Through A Low Ph-Dependent Endocytic Pathway, Luiz C. Kreutz, Mark R. Ackermann
Mark R. Ackermann
The entry pathway of porcine reproductive and respiratory syndrome virus (PRRSV) into MARC-145 cells was investigated using a variety of drugs that interfere with the pH of intracellular vesicles by different mechanisms. Virus entry was assessed by measuring viral RNA replication or production of infectious virus. Chloroquine, ammonium chloride and bafilomycin A1 inhibited RNA replication or production of infectious virus in a dose-dependent manner. These drugs inhibited virus replication when added to the cells prior to, at infection or soon after infection. Moreover, the effect of chloroquine on PRRSV replication was reversible under acidic conditions of the media. Taken together, …
Alimentary And Respiratory Tract Lesions In Eight Medically Fragile Holstein Cattle With Bovine Leukocyte Adhesion Deficiency (Blad), Mark R. Ackermann, M. E. Kehrli Jr., J. A. Laufer, L. T. Nutsz
Alimentary And Respiratory Tract Lesions In Eight Medically Fragile Holstein Cattle With Bovine Leukocyte Adhesion Deficiency (Blad), Mark R. Ackermann, M. E. Kehrli Jr., J. A. Laufer, L. T. Nutsz
Mark R. Ackermann
Lesions in the alimentary tract were studied in eight medically fragile Holstein cattle homozygous for the bovine leukocyte adhesion deficiency (BLAD) allele as determined by polymerase chain reaction and restriction endonuclease analysis. These cattle received institutional medical care but died or were euthanatized because of chronic debilitation associated with diarrhea (6/8) and pneumonia (4/8). The six cattle with diarrhea had acute (n = 3) or chronic (n = 3) intestinal ulcers, but the other two remained relatively healthy for 3 years and did not develop intestinal tract ulcers. Ulcerated areas were present in the small intestine in six animals, and …
Low Calcium Diet And 1,25-Dihydroxyvitamin D3 Infusion Modulate Immune Responses During Mycobacterium Paratuberculosis Infection In Geige Mice, Judith R. Stabel, Jesse P. Goff, Diana L. Whipple, Mark R. Ackermann, Timothy A. Reinhardt
Low Calcium Diet And 1,25-Dihydroxyvitamin D3 Infusion Modulate Immune Responses During Mycobacterium Paratuberculosis Infection In Geige Mice, Judith R. Stabel, Jesse P. Goff, Diana L. Whipple, Mark R. Ackermann, Timothy A. Reinhardt
Mark R. Ackermann
A 12-month study was conducted to evaluate the effects of feeding a low calcium (Ca) diet or 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) infusion on the persistence of Mycobacterium paratuberculosis infection using a mouse model. Male beige mice 6–8 weeks of age were assigned to one of the following treatments: (1) non-infected, (2) infected, (3) non-infected/1,25(OH)2D3, (4) infected/1,25(OH)2D3, and (5) infected/low Ca (0.15%) diet. Infected mice were inoculated intravenously with live M. paratuberculosis. At 1, 6 and 12 months postinfection, mice in Treatments 3 and 4 were implanted subcutaneously with mini-osmotic pumps to deliver 1,25(OH)2D3. Infusion with 1,25(OH)2D3 exacerbated M. paratuberculosis infection in …
Influence Of Chondroitinase On Indirect Hemagglutination Titers And Phagocytosis Of Pasteurella Multocida Serogroups A, D And F, Richard B. Rimler, Karen B. Register, Tibor Magyar, Mark R. Ackermann
Influence Of Chondroitinase On Indirect Hemagglutination Titers And Phagocytosis Of Pasteurella Multocida Serogroups A, D And F, Richard B. Rimler, Karen B. Register, Tibor Magyar, Mark R. Ackermann
Mark R. Ackermann
Capsules of Pasteurella multocida serogroups A, D and F contain mucopolysaccharides which block antigenic determinants and prevent phagocytosis. In this study, capsules of serogroup A, D and F strains of P. multocida were depolymerized by enzyme treatment. Capsule depolymerization of serogroup D and F strains with chondroitinase increased indirect hemagglutination (IHA) test titers and enhanced phagocytosis by swine neutrophils. Capsule depolymerization of serogroup A strains with hyaluronidase increased IHA titers, but depolymerization with chondroitinase did not. When serogroup A strains were treated with a combination of chondroitinase and hyaluronidase, IHA test titers were lower than titers of the same strains …
Nonradioactive Colony Lift-Hybridization Assay For Detection Of Bordetella Bronchiseptica Infection In Swine, K. B. Register, Mark R. Ackermann, D. W. Dyer
Nonradioactive Colony Lift-Hybridization Assay For Detection Of Bordetella Bronchiseptica Infection In Swine, K. B. Register, Mark R. Ackermann, D. W. Dyer
Mark R. Ackermann
Current methods for the isolation and identification of Bordetella bronchiseptica from clinical samples are time-consuming and are based, in part, on subjective observations. We describe the use of a Bordetella-specific DNA probe in a nonradioactive colony lift-hybridization assay for the identification of B. bronchiseptica. Eleven of 82 clinical specimens were found to contain B. bronchiseptica by this method, while only 5 of these were reported to contain the organism when the specimens were analyzed by traditional methods. The chromosomal fragment containing a sequence complementary to the probe appeared to be conserved in B. bronchiseptica isolates from swine from a variety …
Influence Of Inoculation Route On The Carrier State Of Salmonella Choleraesuis In Swine, Jeffrey T. Gray, Paula J. Fedorka-Cray, Thomas J. Stabel, Mark R. Ackermann
Influence Of Inoculation Route On The Carrier State Of Salmonella Choleraesuis In Swine, Jeffrey T. Gray, Paula J. Fedorka-Cray, Thomas J. Stabel, Mark R. Ackermann
Mark R. Ackermann
This study was designed to investigate the carrier state of swine infected with Salmonella choleraesuis. Thirty-five pigs were divided into 3 groups. Groups 1 (n = 15) and 2 (n = 16) were challenged with 108 CFU of S. choleraesuis intranasally or by gastric route, respectively. Group 3 (n = 4) served as uninoculated controls. Pigs were necropsied at 2, 4, 6, and 12 weeks post inoculation. Clinical signs and microscopic lesions were more severe for group 1. Salmonella choleraesuis was recovered from a greater percentage of tissue samples for group 1 versus group 2 at 2, 4, and 6 …
Non-Opsonic Attachment Of Bordetella Bronchiseptica Mediated By Cd11/Cd18 And Cell Surface Carbohydrates, Karen B. Register, Mark R. Ackermann, Marcus E. Kehrli Jr.
Non-Opsonic Attachment Of Bordetella Bronchiseptica Mediated By Cd11/Cd18 And Cell Surface Carbohydrates, Karen B. Register, Mark R. Ackermann, Marcus E. Kehrli Jr.
Mark R. Ackermann
Porcine atrophic rhinitis associated with Bordetella bronchiseptica is characterized by a severe inflammatory response in the mucosa of the nasal turbinates. Initial infiltrates of polymorphonuclear leukocytes (PMN) are followed by accumulations of mononuclear cells. In this report, we have investigated the interaction between porcine PMN and B. bronchiseptica. PMN incubated in PBS with a fluorescently labeled hemagglutinating porcine isolate, but not a non-hemagglutinating variant, had high levels of cell-associated fluorescence as determined by flow cytometry. Light microscopy indicated that most cell-associated bacteria were ingested. Transmission electron microscopy confirmed the presence of intracellular bacteria, which were contained within membrane-bound phagosomes. A …
Pulmonary Lesions In Fetuses Exposed In Utero To Porcine Reproductive And Respiratory Syndrome Virus, Kelly M. Lager, Mark R. Ackermann
Pulmonary Lesions In Fetuses Exposed In Utero To Porcine Reproductive And Respiratory Syndrome Virus, Kelly M. Lager, Mark R. Ackermann
Mark R. Ackermann
The salient features of a porcine reproductive and respiratory syndrome (PRRS) epizootic include respiratory disease followed by reproductive failure. The respiratory component is usually recognized first in finishing pigs or in the breeding herd as a mild flu-like disease with a high morbidity for all ages and high mortality in younger pigs. In breeding herds, acute maternal reproductive failure often follows the onset of respiratory disease. PRRS is characterized by a sudden increase in early farrowings, late-term abortions, stillborn and mummified fetuses, weak neonates with high mortality, late returns to estrus, and repeat breeders. No consistent histopathology has been obseived …
Distribution Of Anti-Cd68 (Ebm11) Immunoreactivity In Formalin-Fixed, Paraffin-Embedded Bovine Tissues, Mark R. Ackermann, B. M. Debey, T. J. Stabel, J. H. Gold, K. B. Register, J. T. Meehan
Distribution Of Anti-Cd68 (Ebm11) Immunoreactivity In Formalin-Fixed, Paraffin-Embedded Bovine Tissues, Mark R. Ackermann, B. M. Debey, T. J. Stabel, J. H. Gold, K. B. Register, J. T. Meehan
Mark R. Ackermann
A commercially acquired anti-human macrophage antibody (anti-CD68; EBM11) was used in an immunocytochemical technique to detect macrophages in formalin-fixed, paraffin-embedded tissues from cattle, pigs, humans, rats, turkeys, dogs, and cats. In healthy cattle, the antibody labeled alveolar macrophages, pulmonary intravascular cells (presumably intravascular macrophages), and macrophage-like cells in other tissues. In bovine lungs infected with Pasteurella haemolytica, EBM11 antibody labeled 95% of alveolar macrophages and macrophages within alveolar septa but only 0–2% of streaming or “oat” leukocytes. Alveolar macrophages were also stained by EBM11 in pigs but not in rats, turkeys, dogs, and cats. The antibody also stained macrophage aggregates …
Infected Cell Types In Ovine Lung Following Exposure To Bovine Respiratory Syncytial Virus, J. T. Meehan, R. C. Cutlip, H. D. Lehmkuhl, J. P. Kluge, Mark R. Ackermann
Infected Cell Types In Ovine Lung Following Exposure To Bovine Respiratory Syncytial Virus, J. T. Meehan, R. C. Cutlip, H. D. Lehmkuhl, J. P. Kluge, Mark R. Ackermann
Mark R. Ackermann
Sixteen adult sheep (ten females, six males obtained from a closed flock at National Animal Disease Center, Ames, IA) were experimentally infected with bovine respiratory syncytial virus strain 375 (BRSV), and lung tissues were stained for viral antigen. Two infected sheep were euthanatized at each of the following post-inoculation times: 12, 24, 36, 48, 72, 96, 144, and 192 hours. Lung, nasal turbinates, trachea, right cranial bronchial and mediastinal lymph nodes, liver, and spleen were collected for histologic evaluation. An indirect immunoperoxidase technique was performed on routine paraffin-embedded sections of lung tissue, trachea, turbinates, and bronchial and mediastinal lymph nodes …
Identification Of Β2 Integrins In Bovine Neutrophils By Scanning Electron Microscopy In The Backscatter Mode And Transmission Electron Microscopy, Mark R. Ackermann, M. E. Kehrli Jr., H. K. Hawkins, J. L. Amenson, J. E. Gallagher
Identification Of Β2 Integrins In Bovine Neutrophils By Scanning Electron Microscopy In The Backscatter Mode And Transmission Electron Microscopy, Mark R. Ackermann, M. E. Kehrli Jr., H. K. Hawkins, J. L. Amenson, J. E. Gallagher
Mark R. Ackermann
Mac-I, LFA-I, and p150,95 are β2 integrin glycoproteins present in the plasma membranes of neutrophils, macrophages, and lymphocytes.
Protection Of Pasteurella Multocida Dermonecrotic Toxin-Challenged Rats By Toxoid-Induced Antibody, R. K. Pettit, R. B. Rimler, Mark R. Ackermann
Protection Of Pasteurella Multocida Dermonecrotic Toxin-Challenged Rats By Toxoid-Induced Antibody, R. K. Pettit, R. B. Rimler, Mark R. Ackermann
Mark R. Ackermann
Two different doses of glutaraldehyde-treated Pasteurella multocida dermonecrotic toxin (PMDT) were used to immunize rats. Rats developed serum IgG antibodies specific for native PMDT, and IgG titers increased with dose and number of toxoid immunizations. Survival rates in both active immunization and passive serum neutralization experiments were dependent on dose of toxoid vaccination and serum levels of anti-PMDT IgG. Vaccination with toxoid prevented weight loss but not leukocytosis and increased complement titers in toxin-challenged rats. Toxoid, itself, induced minimal leukocytosis but no alterations in complement titers or weight gain.
Identification And Prevalence Of A Genetic Defect That Causes Leukocyte Adhesion Deficiency In Holstein Cattle, Dale E. Shuster, Marcus E. Kehrli Jr., Mark R. Ackermann, R. O. Gilbert
Identification And Prevalence Of A Genetic Defect That Causes Leukocyte Adhesion Deficiency In Holstein Cattle, Dale E. Shuster, Marcus E. Kehrli Jr., Mark R. Ackermann, R. O. Gilbert
Mark R. Ackermann
Two point mutations were identified within the gene encoding bovine CD18 in a Holstein calf afflicted with leukocyte adhesion deficiency (LAD). One mutation causes an aspartic acid to glycine substitution at amino acid 128 (D128G) in the highly conserved extracellular region of this adhesion glycoprotein, a region where several mutations have been found to cause human LAD. The other mutation is silent. Twenty calves with clinical symptoms of LAD were tested, and all were homozygous for the D128G allele. In addition, two calves homozygous for the D128G allele were identified during widespread DNA testing, and both were subsequently found to …
Animal Model Of Human Disease: Bovine Leukocyte Adhesion Deficiency: Beta2 Integrin Deficiency In Young Holstein Cattle, Marcus E. Kehrli Jr., Mark R. Ackermann, Dale E. Shuster, Martin J. Van Der Maaten, Frank C. Schmalstieg, Donald C. Anderson, Bonnie J. Hughes
Animal Model Of Human Disease: Bovine Leukocyte Adhesion Deficiency: Beta2 Integrin Deficiency In Young Holstein Cattle, Marcus E. Kehrli Jr., Mark R. Ackermann, Dale E. Shuster, Martin J. Van Der Maaten, Frank C. Schmalstieg, Donald C. Anderson, Bonnie J. Hughes
Mark R. Ackermann
Leukocyte adhesion deficiency (LAD), is a rare, autosomal recessive disorder caused by a lack or partial absence of a family of leukocyte integrins, Mac-1, LFA-1, and p150,95.1 The leukocyte (32) integrins are glycoproteins essential for normal leukocyte-endothelial cell adherence and emigration. Affected children develop recurrent bacterial infections, persistent leukocytosis, severe hypoplasia of lymphoid tissues and isolated neutrophils, eosinophils, macrophages, and lymphocytes have abnormal function when tested in vitro. Most patients without bone marrow transplants die at an early age.
Use Of Rats To Compare Atrophic Rhinitis Vaccines For Protection Against Effects Of Heat-Labile Protein Toxin Produced By Pasteurella Multocida Serogroup D, J. R. Thurston, R. B. Rimler, Mark R. Ackermann, N. F. Cheville
Use Of Rats To Compare Atrophic Rhinitis Vaccines For Protection Against Effects Of Heat-Labile Protein Toxin Produced By Pasteurella Multocida Serogroup D, J. R. Thurston, R. B. Rimler, Mark R. Ackermann, N. F. Cheville
Mark R. Ackermann
Four bacterin-toxoid and three bacterin commercial vaccines against atrophic rhinitis were tested in rats for their capacity to immunize against the lethal and systemic effects of purified heat-labile protein toxin (D-toxin) produced by Pasteurella multocida serogroup D. Only one bacterin-toxoid vaccine stimulated sufficient immunity to prevent the death of all rats challenged with D-toxin. None of the vaccines prevented weight loss, leukocytosis or increases in serum complement titers in rats challenged with D-toxin. Rats provide an inexpensive animal model for testing the capacity of vaccines to generate antitoxic immunity against the lethal and systemic effects of D-toxin.
Light Microscopic And Ultrastructural Pathology Of Seminiferous Tubules Of Rats Given Multiple Doses Of Pasteurella Multocida Group D Protein Toxin, Mark R. Ackermann, James P. Tappe Jr., John R. Thurston, Richard B. Rimler, Dale E. Shuster, Norman F. Cheville
Light Microscopic And Ultrastructural Pathology Of Seminiferous Tubules Of Rats Given Multiple Doses Of Pasteurella Multocida Group D Protein Toxin, Mark R. Ackermann, James P. Tappe Jr., John R. Thurston, Richard B. Rimler, Dale E. Shuster, Norman F. Cheville
Mark R. Ackermann
Male Holtzman rats were given subcutaneous doses of a purified Pasteurella multocida group D heat-labile toxin on alternate days for up to 22 days. Rats were necropsied at 18 days or 36 days (14 days after last dose of toxin) or when moribund, and testicles were taken for histologic and ultrastructural examination. Other selected tissues, including liver and spleen, were taken for histologic examination. Histologically, testicular and splenic lesions occurred more consistently and at much smaller doses when compared with lesions in other target organs such as liver. Testicular and splenic lesions were present in all rats (6/6) given 0.8 …
Bronchiolar Metaplasia And Ulex Europaeus Agglutinin I (Uea-I) Affinity In Mycoplasma Hyopneumoniae-Infected Lungs Of Six Pigs, Mark R. Ackermann, M. C. Debey, B. M. Debey
Bronchiolar Metaplasia And Ulex Europaeus Agglutinin I (Uea-I) Affinity In Mycoplasma Hyopneumoniae-Infected Lungs Of Six Pigs, Mark R. Ackermann, M. C. Debey, B. M. Debey
Mark R. Ackermann
Mycoplasma hyopneumoniae adheres to the cilia and apical plasma membrane of cells that line the trachea, bronchi, and bron ch ioles. Colonization result s in ciliostasis and peribronchial and peribronchi olar nod ular aggregates of lymphocytes and plasma cells.">Altho ugh adhesion-receptor interaction s between the organism and respiratory epithelial cells have been examined, little attention has been given to the morphologic and phenotypic changes that may occur in cells lining bron chioles during infection. In this study, we describe morphologic cha nges and differenc es in lectin staining patt ern s in the bron chi oles from lungs of …
Experimental Model Of Atrophic Rhinitis In Gnotobiotic Pigs, Mark R. Ackermann, R. B. Rimler, J. R. Thurston
Experimental Model Of Atrophic Rhinitis In Gnotobiotic Pigs, Mark R. Ackermann, R. B. Rimler, J. R. Thurston
Mark R. Ackermann
To study the pathogenesis of atrophic rhinitis, gnotobiotic pigs (n = 6) were inoculated intranasally with a sterile sonicate of a toxigenic strain of Bordetella bronchiseptica (0.16 mg of protein per ml) at 5 days of age, and they were then inoculated intranasally with 1 ml (5,250 CFU/ml) of a live, toxigenic strain of Pasteurella multocida at 7 days of age. Pigs were necropsied at 2, 5, 9, 14, 21, and 28 days postinoculation; those pigs necropsied after 5 days had developed turbinate atrophy. Other gnotobiotic pigs received the following inoculation protocols: (i) a sterile sonicate of a nontoxigenic strain …
Colonization Of The Pharyngeal Tonsil And Respiratory Tract Of The Gnotobiotic Pig By A Toxigenic Strain Of Pasteurella Multocida Type D, Mark R. Ackermann, N. F. Cheville, J. E. Gallagher
Colonization Of The Pharyngeal Tonsil And Respiratory Tract Of The Gnotobiotic Pig By A Toxigenic Strain Of Pasteurella Multocida Type D, Mark R. Ackermann, N. F. Cheville, J. E. Gallagher
Mark R. Ackermann
Seven-day-old gnotobiotic pigs were inoculated intranasally with Pasteurella multocida and euthanatized 2, 5, 9, and 14 days after inoculation. Tissues from the oropharynx and respiratory tract of pigs were cultured quantitatively and analyzed microscopically. Pigs remained afebrile and alert, except one that died of acute fibrinopurulent pneumonia. Pasteurella multocida was isolated in greatest numbers from the pharyngeal tonsils, but only in low numbers from turbinate, trachea, lung, spleen, and liver. Significant histologic changes were limited to the tonsil. Infected pigs developed mild tonsillitis with lymphocytic hyperplasia, and accumulation of cell debris and bacteria in crypts. Capsular antigens of P. multocida, …
Lectin Histochemistry Of Trachea And Lung Of Healthy Turkeys (Meleagris Gallopavo) And Turkeys With Pneumonia, Mark R. Ackermann, N. F. Cheville, P. G. Detilleux
Lectin Histochemistry Of Trachea And Lung Of Healthy Turkeys (Meleagris Gallopavo) And Turkeys With Pneumonia, Mark R. Ackermann, N. F. Cheville, P. G. Detilleux
Mark R. Ackermann
Thirteen lectins were used to characterize lectin-binding specificity of glycoconjugates on sections of formalin-fixed lung and trachea from seven normal turkeys, two turkeys with acute pneumonia, and two turkeys with chronic pneumonia. Neuraminidase was used to digest sialic acid residues. One N-acetylgalactosamine-binding lectin and two N-acetylgalactosamine/galactose-binding lectins stained the apical membrane and cytoplasm of multifocal cells that lined air atria and hyperplastic granular cells. Other lectins in these groups stained ciliated cells of the trachea and bronchi and air capillary epithelial cells. Sialic acid residues were on apical surfaces of ciliated and nonciliated tracheal and bronchial lining cells, air capillary …
Immunity Induced In Rats Vaccinated With Toxoid Prepared From Heat-Labile Toxin Produced By Pasteurella Multocida Serogroup D, J. R. Thurston, R. B. Rimler, Mark R. Ackermann, N. F. Cheville, J. M. Sacks
Immunity Induced In Rats Vaccinated With Toxoid Prepared From Heat-Labile Toxin Produced By Pasteurella Multocida Serogroup D, J. R. Thurston, R. B. Rimler, Mark R. Ackermann, N. F. Cheville, J. M. Sacks
Mark R. Ackermann
Rats were vaccinated with a toxoid (D-toxoid) prepared from purified heat-labile toxin (D-toxin) produced by Pasteurella multocida serogroup D. Vaccination of rats with D-toxoid prevented death and other effects of D-toxin (hepatic necrosis, development of elevated leukocyte counts, lymphopenia, neutrophilia, and elevated complement titers) that occurred in phosphate buffered saline (PBS)-vaccinated control rats.
Acute Fibrinopurulent Blepharitis And Conjunctivitis Associated With Staphylococcus Hyicus, Escherichia Coli, And Streptococcus Sp. In Chickens And Turkeys, N. F. Cheville, J. Tappe, Mark R. Ackermann, A. Jensen
Acute Fibrinopurulent Blepharitis And Conjunctivitis Associated With Staphylococcus Hyicus, Escherichia Coli, And Streptococcus Sp. In Chickens And Turkeys, N. F. Cheville, J. Tappe, Mark R. Ackermann, A. Jensen
Mark R. Ackermann
Multiple outbreaks of acute severe fibrinopurulent lesions of the eyelids occurred in chickens and turkeys. Lesions began as tiny foci of epidermal necrosis and ulceration and spread to involve the entire eyelid. Scabs overlying the epidermis contained large gram-positive cocci; lesser numbers of small cocci and gram-negative bacilli were in more superficial areas. Staphylococcus hyicus was isolated from birds in all stages of the disease. Escherichia coli and Streptococcus sp. were isolated only during severe stages; no anaerobic bacterial pathogens were isolated. Vasculitis and perivascular lymphocytic infiltrates in deep layers of the dermis suggested that a staphylococcal toxin may have …
Bovine Ileal Dome Lymphoepithelial Cells: Endocytosis And Transport Of Brucella Abortus Strain 19, Mark R. Ackermann, N. F. Cheville, B. L. Deyoe
Bovine Ileal Dome Lymphoepithelial Cells: Endocytosis And Transport Of Brucella Abortus Strain 19, Mark R. Ackermann, N. F. Cheville, B. L. Deyoe
Mark R. Ackermann
Ligated ileal loops of calves were inoculated with Brucella abortus and examined at 2, 4, 6, 10, and 24 hours post-inoculation. B. abortus was identified by light and electron microscopy using immunoperoxidase and antibody-coated colloidal gold techniques. B. abortus was detected in vesicles, phagolysosomes, and large vacuoles of lymphoepithelial cells. Numbers of intracellular bacteria decreased with time after inoculation. B. abortus was also seen between and below lymphoepithelial cells and free in the dome interstitium and intestinal lymph vessels. Neutrophils and macrophages in both epithelium and lamina propria contained intact or degraded bacteria within phagosomes, phagolysosomes, and multivesicular bodies. These …