Medicine and Health Sciences Commons^™

Regulation Of Rac1 Activation By The Low Density Lipoprotein Receptor–Related Protein, Zhong Ma, Keena S. Thomas, Donna J. Webb, Radim Moravec, Ana Maria Salicioni, Wendy M. Mars, Steven L. Gonias

Ana Maria Salicioni

The low density lipoprotein receptor–related protein (LRP-1) binds and mediates the endocytosis of multiple ligands, transports the urokinase-type plasminogen activator receptor (uPAR) and other membrane proteins into endosomes, and binds intracellular adaptor proteins involved in cell signaling. In this paper, we show that in murine embryonic fibroblasts (MEFs) and L929 cells, LRP-1 functions as a major regulator of Rac1 activation, and that this activity depends on uPAR. LRP-1–deficient MEFs demonstrated increased Rac1 activation compared with LRP-1–expressing MEFs, and this property was reversed by expressing the VLDL receptor, a member of the same gene family as LRP-1, with overlapping ligand-binding specificity. …

Short Communication: Relationship Between Body Growth And Mammary Development In Dairy Heifers, L F. Silva, M J. Vandehaar, Brian K. Whitlock, R P. Radcliff, H A. Tucker

Brian K Whitlock, PhD, DVM, DACT

Our objective was to determine if prepubertal rate of body weight (BW) gain, independent of diet, was related to mammary development of dairy heifers. Data from two studies recently conducted at Michigan State University were used to identify factors, within a dietary treatment group, that would account for variation in first lactation milk production or amount of mammary parenchymal DNA at the time of puberty. Factors analyzed for variation in milk production during first lactation were: postpartum BW, prepubertal BW gain, gestational BW gain, postpartum BW gain, body condition score (BCS) at breeding, and BCS at calving. Factors analyzed for …

Retinal Histopathology Of An Xlrp Carrier With A Mutation In The Rpgr Exon Orf15, Gustavo Aguirre, Beverly Yashar, Sinoj John, Julie Smith, K Breuer, S Hiriyanna, Anand Swaroop, Ann Milam

Gustavo D. Aguirre, VMD, PhD

Encapsulated Cell-Based Delivery Of Cntf Reduces Photoreceptor Degeneration In Animal Models Of Retinitis Pigmentosa, Weng Tao, Rong Wen, Moses B. Goddard, Sandy D. Sherman, Pam J. O'Rourke, Paul F. Stabila, William J. Bell, Brenda J. Dean, Konrad A. Kauper, Veronica A. Budz, William G. Tsiaras, Gregory M. Acland, Sue Pearce-Kelling, Alan Laties, Gustavo D. Aguirre

Gustavo D. Aguirre, VMD, PhD

Effect Of Dietary Protein On Prepubertal Mammary Development In Rapidly Growing Dairy Heifers, Brian K. Whitlock, M J. Vandehaar, L F. Silva, H A. Tucker

Brian K Whitlock, PhD, DVM, DACT

The objective was to determine whether increased dietary protein would enhance mammary development in prepubertal heifers fed for rapid body growth (1.2 kg/d). Fifty-four Holstein heifers (weighing approximately 134 kg) were assigned to one of three treatments. Heifers were fed a total mixed ration with metabolizable energy at 2.85 Mcal/kg and metabolizable protein at low, standard, or high concentrations (37, 41, or 44 g/Mcal of metabolizable energy, respectively) from 3.5 mo of age until slaughter at approximately 46 d after puberty. Heifers fed low, standard, and high protein gained 1130, 1170, and 1180 g/d, respectively. Dietary protein did not affect …

Complement Resistance, As Determined By Viable Count And Flow Cytometric Methods, And Its Association With The Presence Of Iss And The Virulence Of Avian Escherichia Coli, Lisa K. Nolan, Catherine W. Giddings, Shelley M. Horne, Curt Doetkott, Penelope S. Gibbs, Richard E. Wooley, Steven L. Foley

Lisa K. Nolan

Previous work in our labs has shown that avian Escherichia coli virulence is correlated with resistance to complement. Also, our studies have revealed that the presence of the increased serum survival gene (iss), known to contribute to the complement resistance and virulence of mammalian E. coli, may predict the virulent nature of an avian E. coli isolate. This relationship warrants further research, but further clarification of the relationship among virulence, complement resistance, and iss sequences requires use of complement susceptibility assays. Such assays, unfortunately, are labor-intensive, expensive, and difficult to perform. In the present study, the results of two complement …

Location Of Increased Serum Survival Gene And Selected Virulence Traits On A Conjugative R Plasmid In An Avian Escherichia Coli Isolate, Timothy J. Johnson, Catherine W. Giddings, Shelley M. Horne, Penelope S. Gibbs, Richard E. Wooley, Jerod Skyberg, Pam Olah, Ronda Kercher, Julie S. Sherwood, Steven L. Foley, Lisa K. Nolan

Lisa K. Nolan

Avian colibacillosis is a costly disease for the poultry industry. The mechanisms of virulence employed by the etiologic agent of this disease remain ill defined. However, accumulated evidence suggests that complement resistance and the presence of the increased serum survival gene (iss) in an avian Escherichia coli isolate may be indicative of its ability to cause disease. This association of iss with the E. coli implicated in avian disease may mean that iss and/or, perhaps, the genes associated with it are important contributors to avian E. coli virulence. For this reason, we have begun a search for iss's location in …

Virulence Factors Of Escherichia Coli From Cellulitis Or Colisepticemia Lesions In Chickens, J. S. Jeffrey, Lisa K. Nolan, K. H. Tonooka, S. Wolfe, C. W. Giddings, S. M. Horne, S. L. Foley, A. M. Lynne, J. O. Ebert, L. M. Elijah, G. Bjorklund, S. J. Pfaff-Mcdonough, R. S. Singer, C. Doetkott

Lisa K. Nolan

This study was designed to compare virulence factors of cellulitis-derived Escherichia coli to colisepticemic E. coli in order to clarify whether E. coli associated with cellulitis comprise a unique subset of pathogenic E. coli. Isolates were tested for serotype, capsule, aerobactin production, colicin production, the presence of the iss gene, and serum resistance. Untypable isolates made up the greatest percentage of each group. Serotypes O2 and O78 were the most commonly identified among both groups of isolates. No statistical differences in the distribution of aerobactin or colicin production, capsule, or iss gene were observed between groups. Cluster analysis showed that …

Variability Of Placental Expression Of Cyclin E Low Molecular Weight Variants, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Ja Keenan, Rf Elder

Maria Cekanova MS, RNDr, PhD

No abstract provided.

Canine Cngb3 Mutations Establish Cone Degeneration As Orthologous To The Human Achromatopsia Locus Achm3, Duska J. Sidjanin, Jennifer K. Lowe, John L. Mcelwee, Bruce S. Milne, Taryn M. Phippen, David R. Sargan, Gustavo D. Aguirre, Gregory M. Acland, Elaine A. Ostrander

Gustavo D. Aguirre, VMD, PhD