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Transmission Of Ranavirus Between Ectothermic Vertebrate Hosts, R Brenes, Matt J. Gray, T B. Waltzek, Rebecca P. Wilkes, Debra L. Miller
Transmission Of Ranavirus Between Ectothermic Vertebrate Hosts, R Brenes, Matt J. Gray, T B. Waltzek, Rebecca P. Wilkes, Debra L. Miller
Debra L Miller
Transmission is an essential process that contributes to the survival of pathogens. Ranaviruses are known to infect different classes of lower vertebrates including amphibians, fishes and reptiles. Differences in the likelihood of infection among ectothermic vertebrate hosts could explain the successful yearlong persistence of ranaviruses in aquatic environments. The goal of this study was to determine if transmission of a Frog Virus 3 (FV3)-like ranavirus was possible among three species from different ectothermic vertebrate classes: Cope's gray treefrog (Hyla chrysoscelis) larvae, mosquito fish (Gambusia affinis), and red-eared slider (Trachemys scripta elegans). We housed individuals previously exposed to the FV3-like ranavirus …
Seasonal Trends In Nesting Leatherback Turtle (Dermochelys Coriacea) Serum Proteins Further Verify Capital Breeding Hypothesis, Justin R. Perrault, J Wyneken, A Page-Karjian, A Merrill, Debra L. Miller
Seasonal Trends In Nesting Leatherback Turtle (Dermochelys Coriacea) Serum Proteins Further Verify Capital Breeding Hypothesis, Justin R. Perrault, J Wyneken, A Page-Karjian, A Merrill, Debra L. Miller
Debra L Miller
Serum protein concentrations provide insight into the nutritional and immune status of organisms. It has been suggested that some marine turtles are capital breeders that fast during the nesting season. In this study, we documented serum proteins in neo-phyte and remigrant nesting leatherback sea turtles (Dermochelys coriacea). This allowed us to establish trends across the nesting season to determine whether these physiological parameters indicate if leatherbacks forage or fast while on nesting grounds. Using the biuret method and agarose gel electrophoresis, total serum protein (median=5.0g/dl) and protein fractions were quantified and include pre-albumin (median=0.0g/dl), albumin (median=1.81g/dl),α1-globulin (median=0.90g/dl),α2-globulin(median]=0.74g/dl), total α-globulin (median=1.64g/dl), …
Characterization Of A Piscirickettsiosis-Like Disease In Hawaiian Tilapia., M. J. Mauel, Debra L. Miller, K. Frazier, A. Liggett, E. Styer, D. Montgomery-Brock, J. Brock
Characterization Of A Piscirickettsiosis-Like Disease In Hawaiian Tilapia., M. J. Mauel, Debra L. Miller, K. Frazier, A. Liggett, E. Styer, D. Montgomery-Brock, J. Brock
Debra L Miller
In 1994, tilapia (Oreochromis mossambicus and Sarotherodon melanotheron) in wild and farmed populations on Oahu, Hawaii, USA, began to die of an unknown disease that was similar but not identical to piscirickettsiosis in salmonids. Only tilapia were affected. Diseased tilapia often swam erratically and had trouble staying at depth. Scattered cutaneous haemorrhage and exophthalmia were often noted. In many cases, fish were found dead with no clinical signs. Gills exhibited epithelial hyperplasia with severe multifocal consolidation of secondary lamellae. Multiple granulomas were observed in the gills, spleen, kidney, choroid gland and testes, but not in the liver. Tilapia mortalities occurred …