Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Medicine and Health Sciences
Chimeric Protein A/G Conjugate For Detection Of Anti–Toxoplasma Gondii Immunoglobulin G In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
Chimeric Protein A/G Conjugate For Detection Of Anti–Toxoplasma Gondii Immunoglobulin G In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
John J Schaefer
Serological testing for toxoplasmosis diagnosis remains the method of choice in human medicine due to the accessibility of the requisite sample, the difficulty in predicting the parasite’s location in the host for direct detection, and the availability of established commercial methods. In veterinary medicine, although the first 2 conditions are unchanged, there is a need for commercially produced test methods that are validated for Toxoplasma gondii detection across the range of animal species that can serve as intermediate hosts. The development of such a serological method for animals would allow the diagnosis of toxoplasmosis in individual animals and a higher …
Modification Of A Commercial Toxoplasma Gondii Immunoglobulin G Enzyme-Linked Immunosorbent Assay For Use In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
Modification Of A Commercial Toxoplasma Gondii Immunoglobulin G Enzyme-Linked Immunosorbent Assay For Use In Multiple Animal Species, John J. Schaefer, Holly A. White, Stephanie L. Schaaf, Hussni O. Mohammed, Susan E. Wade
John J Schaefer
A challenge faced by veterinary diagnosticians in serologic analysis for exposure to pathogens is the need for a protein conjugate capable of antibody attachment in many animal species. The advent of protein conjugates that are less specific in nature allows diagnosis across many species with little or no modification of technique. Toxoplasma gondii is an organism of veterinary interest that has been demonstrated to infect a plethora of warm-blooded animals. However, the serologic tests available for simultaneous diagnosis in this broad range are limited in number. The current study examined the use of an immunoglobulin G enzyme-linked immunosorbent assay (ELISA) …
Efficacy Of A Doxycycline Treatment Regimen Initiated During Three Different Phases Of Experimental Ehrlichiosis, Jennifer C. Mcclure, Michelle L. Crothers, John J. Schaefer, Patrick D. Stanley, Glen R. Needham, S A. Ewing, Roger W. Stich
Efficacy Of A Doxycycline Treatment Regimen Initiated During Three Different Phases Of Experimental Ehrlichiosis, Jennifer C. Mcclure, Michelle L. Crothers, John J. Schaefer, Patrick D. Stanley, Glen R. Needham, S A. Ewing, Roger W. Stich
John J Schaefer
Doxycycline is the treatment of choice for canine monocytic ehrlichiosis (CME), a well-characterized disease and valuable model for tick-borne zoonoses. Conflicting reports of clearance of Ehrlichia canis after treatment with doxycycline suggested that the disease phase during which treatment is initiated influences outcomes of these treatments. The purpose of this study was to evaluate the efficacy of a 28-day doxycycline regimen for clearance of experimental E. canis infections from dogs treated during three phases of the disease. Ten dogs were inoculated with blood from E. canis carriers and treated with doxycycline during acute, subclinical, or chronic phases of CME. Daily …