Life Sciences Commons^™

Meiotic Cohesion Requires Accumulation Of Ord On Chromosomes Before Condensation, Eric M. Balicky, Matthew W. Endres, Cary Lai, Sharon E. Bickel

Dartmouth Scholarship

Cohesion between sister chromatids is a prerequisite for accurate chromosome segregation during mitosis and meiosis. To allow chromosome condensation during prophase, the connections that hold sister chromatids together must be maintained but still permit extensive chromatin compaction. In Drosophila, null mutations in the orientation disruptor (ord) gene lead to meiotic nondisjunction in males and females because cohesion is absent by the time that sister kinetochores make stable microtubule attachments. We provide evidence that ORD is concentrated within the extrachromosomal domains of the nuclei ofDrosophila primary spermatocytes during early G2, but accumulates on the meiotic chromosomes by …

Regulation Of Flagellar Dynein By Calcium And A Role For An Axonemal Calmodulin And Calmodulin-Dependent Kinase, Elizabeth F. Smith

Dartmouth Scholarship

Ciliary and flagellar motility is regulated by changes in intraflagellar calcium. However, the molecular mechanism by which calcium controls motility is unknown. We tested the hypothesis that calcium regulates motility by controlling dynein-driven microtubule sliding and that the central pair and radial spokes are involved in this regulation. We isolated axonemes from Chlamydomonasmutants and measured microtubule sliding velocity in buffers containing 1 mM ATP and various concentrations of calcium. In buffers with pCa > 8, microtubule sliding velocity in axonemes lacking the central apparatus (pf18 and pf15) was reduced compared with that of wild-type axonemes. In contrast, at …

Ube1l Is A Retinoid Target That Triggers Pml/Rarα Degradation And Apoptosis In Acute Promyelocytic Leukemia, Sutisak Kitareewan, Ian Pitha-Rowe, David Sekula, Christopher H. Lowrey, Michael J. Nemeth, Todd R. Golub, Sarah J. Freemantle, Ethan Dmitrovsky

Dartmouth Scholarship

All-trans-retinoic acid (RA) treatment induces remissions in acute promyelocytic leukemia (APL) cases expressing the t(15;17) product, promyelocytic leukemia (PML)/RA receptor α (RARα). Microarray analyses previously revealed induction of UBE1L (ubiquitin-activating enzyme E1-like) after RA treatment of NB4 APL cells. We report here that this occurs within 3 h in RA-sensitive but not RA-resistant APL cells, implicating UBE1L as a direct retinoid target. A 1.3-kb fragment of the UBE1L promoter was capable of mediating transcriptional response to RA in a retinoid receptor-selective manner. PML/RARα, a repressor of RA target genes, abolished this UBE1L promoter activity. A hallmark of …

Erv14p Directs A Transmembrane Secretory Protein Into Copii-Coated Transport Vesicles, Jacqueline Powers, Charles Barlowe

Dartmouth Scholarship

Erv14p is a conserved integral membrane protein that traffics in COPII-coated vesicles and localizes to the early secretory pathway in yeast. Deletion of ERV14 causes a defect in polarized growth because Axl2p, a transmembrane secretory protein, accumulates in the endoplasmic reticulum and is not delivered to its site of function on the cell surface. Herein, we show that Erv14p is required for selection of Axl2p into COPII vesicles and for efficient formation of these vesicles. Erv14p binds to subunits of the COPII coat and binding depends on conserved residues in a cytoplasmically exposed loop domain of Erv14p. When mutations are …

Genomic Analysis Of Homotypic Vacuole Fusion, E Scott Seeley, Masashi Kato, Nathan Margolis, William Wickner, Gary Eitzen

Dartmouth Scholarship

Yeast vacuoles undergo fission and homotypic fusion, yielding one to three vacuoles per cell at steady state. Defects in vacuole fusion result in vacuole fragmentation. We have screened 4828 yeast strains, each with a deletion of a nonessential gene, for vacuole morphology defects. Fragmented vacuoles were found in strains deleted for genes encoding known fusion catalysts as well as 19 enzymes of lipid metabolism, 4 SNAREs, 12 GTPases and GTPase effectors, 9 additional known vacuole protein-sorting genes, 16 protein kinases, 2 phosphatases, 11 cytoskeletal proteins, and 28 genes of unknown function. Vacuole fusion and vacuole protein sorting are catalyzed by …

Neurospora Clock-Controlled Gene 9 (Ccg-9) Encodes Trehalose Synthase: Circadian Regulation Of Stress Responses And Development, Mari L. Shinohara, Alejandro Correa, Deborah Bell-Pedersen, Jay C. Dunlap, Jennifer J. Loros

Dartmouth Scholarship

The circadian clock of Neurospora crassa regulates the rhythmic expression of a number of genes encoding diverse functions which, as an ensemble, are adaptive to life in a rhythmic environment of alternating levels of light and dark, warmth and coolness, and dryness and humidity. Previous differential screens have identified a number of such genes based solely on their cycling expression, including clock-controlled gene 9 (ccg-9). Sequence analysis now shows the predicted CCG-9 polypeptide to be homologous to a novel form of trehalose synthase; as such it would catalyze the synthesis of the disaccharide trehalose, which plays an important …

The Unblazed Trail: Bioinformatics And The Protection Of Genetic Knowledge, Lawrence M. Sung

Faculty Scholarship

No abstract provided.

2002 Nebraska Swine Report, Duane E. Reese

University of Nebraska-Lincoln Extension: Historical Materials

This publication was prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching and Research programs. It deals with the results that were done in reproduction, breeding, health, nutrition, economics and housing of swine.

Marek's Disease Virus (Mdv) Encodes An Interleukin-8 Homolog (Vil-8): Characterization Of The Vil-8 Protein And A Vil-8 Deletion Mutant Mdv, Mark S. Parcells, Su-Fang Lin, Robert L. Dienglewicz, Vladimir Majerciak, Dan R. Robinson, Hua-Chien Chen, Zining Wu, George R. Dubyak, Peter Brunovskis, Henry D. Hunt

Dartmouth Scholarship

Chemokines induce chemotaxis, cell migration, and inflammatory responses. We report the identification of an interleukin-8 (IL-8) homolog, termed vIL-8, encoded within the genome of Marek's disease virus (MDV). The 134-amino-acid vIL-8 shares closest homology to mammalian and avian IL-8, molecules representing the prototype CXC chemokine. The gene for vIL-8 consists of three exons which map to the BamHI-L fragment within the repeats flanking the unique long region of the MDV genome. A 0.7-kb transcript encoding vIL-8 was detected in an n-butyrate-treated, MDV-transformed T-lymphoblastoid cell line, MSB-1. This induction is essentially abolished by cycloheximide and herpesvirus DNA polymerase inhibitor phosphonoacetate, indicating …

Studies On The Formation Of Dna-Cationic Lipid Composite Films And Dna Hybridization In The Composites, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Krishna N. Ganesh

Faculty Works

The formation of composite films of double-stranded DNA and cationic lipid molecules (octadecylamine, ODA) and the hybridization of complementary single-stranded DNA molecules in such composite films are demonstrated. The immobilization of DNA is accomplished by simple immersion of a thermally evaporated ODA film in the DNA solution at close to physiological pH. The entrapment of the DNA molecules in the cationic lipid film is dominated by attractive electrostatic interaction between the negatively charged phosphate backbone of the DNA molecules and the protonated amine molecules in the thermally evaporated film and has been quantified using quartz crystal microgravimetry (QCM). Fluorescence studies …

Characterization Of A Non-Ribosomal Peptide From Agrobacterium Tumefaciens, Graham Strub

Honors Theses

The current efforts underway to sequence the genome of Agrobacterium tumefaciens have led to the discovery of a large region of DNA (52kb) with a strong homology to several polyketide synthetase genes. This was a matter of great interest to the Agrobacterium project; no current literature documents the production of a polyketide or non:iibosomal peptide from Agrobacterium. Furthermore, the multi-domain enzymes coded by these polyketide synthetase genes in other bacterial species are responsible for synthesizing several toxins, many which act as antibiotic agents {8, 9, 12, 16). This was also of great interest, since Agrobacterium is not known to produce …

The Mannose-Sensitive Hemagglutinin Of Vibrio Cholerae Promotes Adherence To Zooplankton, Deborah A. Chiavelli, Jane W. Marsh, Ronald K. Taylor

Dartmouth Scholarship

The bacterium Vibrio cholerae, the etiological agent of cholera, is often found attached to plankton, a property that is thought to contribute to its environmental persistence in aquatic habitats. The V. cholerae O1 El Tor biotype and V. cholerae O139 strains produce a surface pilus termed the mannose-sensitive hemagglutinin (MSHA), whereas V. cholerae O1 classical biotype strains do not. Although V. cholerae O1 classical does not elaborate MSHA, the gene is present and expressed at a level comparable to that of the other strains. Since V. cholerae O1 El Tor and V. cholerae O139 have displaced V. cholerae O1 classical …

Consent To The Use Of Stored Dna For Genetics Research: A Survey Of Attitudes In The Jewish Population, Marc D. Schwartz, Karen H. Rothenberg, Linda Joseph, Judith Benkendorf, Caryn Lerman

Faculty Scholarship

No abstract provided.

Nuclear Export Of 60s Ribosomal Subunits Depends On Xpo1p And Requires A Nuclear Export Sequence-Containing Factor, Nmd3p, That Associates With The Large Subunit Protein Rpl10p, Olivier Gadal, Daniela Strau, Jacques Kessl, Bernard Trumpower

Dartmouth Scholarship

Nuclear export of ribosomes requires a subset of nucleoporins and the Ran system, but specific transport factors have not been identified. Using a large subunit reporter (Rpl25p-eGFP), we have isolated several temperature-sensitive ribosomal export (rix) mutants. One of these corresponds to the ribosomal protein Rpl10p, which interacts directly with Nmd3p, a conserved and essential protein associated with 60S subunits. We find that thermosensitive nmd3 mutants are impaired in large subunit export. Strikingly, Nmd3p shuttles between the nucleus and cytoplasm and is exported by the nuclear export receptor Xpo1p. Moreover, we show that export of 60S subunits is Xpo1p dependent. We …

Evaluation Of The Major Histocompatibility Complex (Mhc) In Cranes: Applications To Conservation Efforts, Susan I. Jarvi, Marcia M. Miller, Ronald M. Gotol, George F. Gee, W. Elwood Briles

Proceedings of the North American Crane Workshop

Although there have been heated discussions concerning the relative importance of using Mhc diversity as a basis for selecting breeders in conservation projects, most parties agree that the genetic variability residual in an endangered species should be maintained through genetic management, if at all possible. Substantial evidence exists (particularly in birds) documenting the influences of specific Mhc haplotypes on disease outcome and also that those individuals which are heterozygous for Mhc alleles appear to have an advantage for survival over those that are homozygous. Thus, conservation of genetic variability of the Mhc is likely important for the preservation of fitness, …

2001 Nebraska Swine Report, Duane E. Reese

University of Nebraska-Lincoln Extension: Historical Materials

This publication was prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching and Research programs. It deals with the results that were done in reproduction, breeding, health, nutrition, economics and housing of swine.

Comparative Genetics Of Seven Plants Endemic To Florida’S Lake Wales Ridge, Eric S. Menges, Rebecca W. Dolan, Rebecca Yahr, Doria R. Gordan

Scholarship and Professional Work - LAS

Here we submit that mathematical tools used in population viability analysis can be used in conjunction with floristic and faunistic surveys to predict changes in biogeographic range. We illustrate our point by summarizing the results of a demographic study of Lobelia boykinii. In this study we used deterministic and stochastic matrix models to estimate the growth rate and to predict the time to extinction for three populations growing in the Carolina bays. The stochastic model better discriminated among the fates of the three populations. It predicted extinction for two populations in the next 25 years but no extinction of the …

Hybridization Of Dna By Sequential Immobilization Of Oligonucleotides At The Air-Water Interface, Murali Sastry, Vidya Ramakrishnan, Mrunalini Pattarkine, Anand Gole, K. N. Ganesh

Faculty Works

The hybridization of DNA by sequential electrostatic and hydrogen-bonding immobilization of single-stranded complementary oligonucleotides at the air-water interface with cationic Langmuir monolayers is demonstrated. The complexation of the single-stranded DNA molecules with octadecylamine (ODA) Langmuir monolayers was followed in time by monitoring the pressure-area isotherms. A large (and slow) expansion of the ODA monolayer was observed during each stage of complexation in the following sequence: primary single-stranded DNA followed by complementary single-stranded DNA followed by the intercalator, ethidium bromide. Langmuir-Blodgett (LB) films of the ODA-DNA complex were formed on different substrates and characterized using quartz-crystal microgravimetry (QCM), Fourier transform infrared …

Effects Of Genetic Type And Protein Levels On Growth Of Swine, O.W. Robison, L. L. Christian, R. Goodwin, R. K. Johnson, J. W. Mabry, R. K. Miller, M. D. Tokach

Department of Animal Science: Faculty Publications

A total of 1,588 pigs representing six genetic lines were included in this study. These lines were selected to represent a wide range of biological types for appetite, rate and composition of gain, and meat quality traits. Each line was fed four different diets differing in lysine content. Barrows and gilts were used in the experiment, and pigs were randomly assigned to come off test at one of three weights (113.6, 131.8, or 150 kg). Rates of increase in weight, backfat thickness, and longissimus muscle area were essentially linear. Genetic type and diet influenced (P < .05) rates of change in weight, backfat thickness, and longissimus muscle area and 10th rib fat depth, carcass longissimus muscle area, and dressing percentage. Genetic type × diet interactions were significant for weight change and 10th rib fat. As off-test-weight class increased fat and longissimus muscle area increased in linear fashion. In general, interactions associated with diet resulted from feeding the diet lowest in lysine. There was little evidence of genetic type × diet interactions. If those interactions that tested significant are real, they are a result of extremes in both genetic type (high fatness) and lysine level (low). Slaughtering pigs at heavier weights results in no change in rate of gain over the feeding period and linear increases in longissimus muscle area and backfat thickness.

Genetic Correlations For Daily Gain Between Ram And Ewe Lambs Fed In Feedlot Conditions And Ram Lambs Fed In Pinpointer Units, L. Dale Van Vleck, Kreg A. Leymaster, Thomas G. Jenkins

Department of Animal Science: Faculty Publications

When performance is recorded in automated facilities that measure feed intake of individual lambs that are penned in a group, such as Pinpointer units, a legitimate question is the degree to which daily gain is genetically correlated with daily gain achieved under feedlot conditions. Lambs were from a composite population (½Columbia, ¼ Suffolk, and ¼ Hampshire germplasm) and of the F₂ or more advanced generations. Data were daily gains of 1,101 rams (PR) fed in Pinpointer units (11 to 17 wk of age) and 2,021 rams (FR) and 3,513 ewes (FE) fed under feedlot conditions (9- or 10-wk period …

Crop Updates 2000 - Weeds, Vanessa Stewart, David Minkey, Abul Hashem, Glen Riethmuller, Martin Harries, Peter Newman, Cameron Weeks, Harmohinder S. Dhammu, Aik Cheam, David Bowran, Terry Piper, Alexandra Wallace, Bill Roy, Keith L. Devenish, Lisa J. Leaver, Brad Rayner, Mike Collins, Marta Monardino, David Pannell, Stephen Powles, Sally Peltzer, Michael Walsh, Charles Boyle, P. Neve, D. Lorraine-Colwill, C. Preston, Art Diggle, Rick Llewellyn, Ryan Duane, Siew Lee, David Nicholson, Peter Carlton, Stewart Smith, Bill Crabtree, Gordon Cumming, David Cameron, Mike Jackson, Scott Paton, John R. Peirce, Greg Shea

Crop Updates

This session covers thirty six papers from different authors:

INTRODUCTION, Vanessa Stewart Agriculture Western Australia

INTEGRATED WEED MANAGEMENT

1. Effect of seeding density, row spacing and Trifluralin on the competitive ability of Annual Ryegrass in a minimum tillage system, David Minkey, Abul Hashem, Glen Riethmuller and Martin Harries, Agriculture Western Australia

2. High wheat seeding rates coupled with narrow row spacing increases yield and suppresses grass, Peter Newman¹ and Cameron Weeks²,¹Agronomist, Elders Limited ²Mingenew/Irwin Group

3. Resistant ryegrass management in a wheat – lupin rotation, Abul Hashem, Harmohinder S. Dhammu, Aik Cheam, David Bowran and …

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Cell Cycle-Dependent Binding Of Yeast Heat Shock Factor To Nucleosomes, Christina Bourgeois Venturi, Alexander M. Erkine, David S. Gross

Scholarship and Professional Work – COPHS

In the nucleus, transcription factors must contend with the presence of chromatin in order to gain access to their cognate regulatory sequences. As most nuclear DNA is assembled into nucleosomes, activators must either invade a stable, preassembled nucleosome or preempt the formation of nucleosomes on newly replicated DNA, which is transiently free of histones. We have investigated the mechanism by which heat shock factor (HSF) binds to target nucleosomal heat shock elements (HSEs), using as our model a dinucleosomal heat shock promoter (hsp82-ΔHSE1). We find that activated HSF cannot bind a stable, sequence-positioned nucleosome in G₁-arrested …

2000 Nebraska Swine Report, Rodger K. Johnson

University of Nebraska-Lincoln Extension: Historical Materials

This publication was prepared by the staff in Animal Science and cooperating Departments for use in Extension, Teaching and Research programs. It deals with the results that were done in reproduction, breeding, health, nutrition, economics and housing of swine.

Genetics And Some Aging-Related Mechanisms, Şükran Çakir

Turkish Journal of Zoology

Aging can be defined as the time-dependent decline of physiological functions of an organism. Aging, a multifactorial process composed of both genetic and environmental components, is a highly complex biologic phenomenon of great importance. The details of the mechanisms leading to aging are not yet known. All changes in the process of aging have a cellular basis, and perhaps aging should be studied, fundamantally, at the cellular level under defined and controlled environmental conditions. Some genetic approaches have promise for the understanding of this multifactorial process. In this review, some causal hypotheses related to oxidative damage, caloric restriction, mitochondrial mutations, …

On The Polymorphic Meadow Spittlebug, Philaenus Spumarius (L.) (Homoptera: Cercopidae), Selçuk Yurtsever

Turkish Journal of Zoology

Due to its interesting biological aspects, the meadow spittlebug Philaenus spumarius has received great attention from biologists for decades. It has been one of the extensively studied species in ecology and genetics. This homopteran insect shows very high habitat diversity and therefore has a wide global distribution. Adults exhibit a heritable colour/pattern polymorphism on the dorsal surface throughout its range. A similar colour/pattern variation also occurs in certain ventral parts. Recent laboratory studies have dealt with its polyandrous aspect that is, females may mate several times with different males and the offspring of a single female may be fathered, therefore, …

Scientific Investigation Of The Plant Pathogen Agrobacterium Tumefaciens, Casey M. Flanagan, Christopher B. Crowell Ii, Bryan P. Markelz

Honors Theses

A. tumefaciensis a plant pathogen and causative agent of Crown Gall Disease, an ailment that results in the development of tumors in plants. It is a unique organism in that it is the only known non-viral pathogen that transfers its DNA into the host cell. Consequently, the majority of the research conducted on A. tumefaciensinvestigates its mechanism of infection. However, A. tumefaciensis also rare to the bacterial world in that its genome is comprised of two chromosomes: one circular and one linear. Most other bacteria only have one circular chromosome. This area of research has been grossly overlooked and therefore …

Bridging The Gap Between Life Insurer And Consumer In The Genetic Testing Era: The Rf Proposal, Christopher M. Keefer

Indiana Law Journal

No abstract provided.

Phenotypic Expressions Of Ccr5-Δ32/Δ32 Homozygosity, Giang T. Nguyen, Mary Carrington, Judith A. Beeler, Michael Dean, Louis M. Aledort, Philip M. Blatt, Alan R. Cohen, Donna Dimichele, M. Elaine Eyster, Craig M. Kessler, Barbara Konkle, Cindy Leissinger, Naomi Luban, Stephen J. O'Brien, James J. Goedert, Thomas R. O'Brien

Biology Faculty Articles

Objective: As blockade of CC-chemokine receptor 5 (CCR5) has been proposed as therapy for HIV-1, we examined whether the CCR5-Δ32/Δ32 homozygous genotype has phenotypic expressions other than those related to HIV-1.

Design: Study subjects were white homosexual men or men with hemophilia who were not infected with HIV-1. In this study, 15 CCR5-Δ32/Δ32 homozygotes were compared with 201 CCR5 wild-type (+/+) subjects for a wide range of clinical conditions and laboratory assay results ascertained during prospective cohort studies and routine clinical care. CCR5-Δ32 genotype was determined by polymerase chain reaction, followed by single-stranded conformational polymorphism analysis.

Results: …

Implications Of Cloning For Breed Improvement Strategies: Are Traditional Methods Of Animal Improvement Obsolete?, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Can the optimum animal be defined? Will that definition change over time, by location, by market demand? First, assume what may be impossible, that the perfect animal can be defined or that only a limited number of definitions of “perfect” are needed. Then, can the “perfect” animal to match a definition be found? Suppose such an animal is found. Then the question to be answered before trying to clone as a method of genetic improvement becomes “Is the animal perfect because of phenotype or genotype?” In other words, the P = G + E problem exists, which requires traditional methods …

Genotypic Expression At Different Ages: Ii. Wool Traits Of Sheep, H. Okut, C. M. Bromley, L. Dale Van Vleck, G. D. Snowder

Department of Animal Science: Faculty Publications

Genetic parameters for wool traits for Columbia, Polypay, Rambouillet, and Targhee breeds of sheep were estimated with single- and multiple-trait analyses using REML with animal models. Traits considered were fleece grade, fleece weight, and staple length. Total number of observations ranged from 11,673 to 34,746 for fleece grade and fleece weight and from 3,500 to 11,641 for staple length for the four breeds. For single-trait analyses, data were divided by age of ewe: young ages (age of 1 yr), middle ages (ages of 2 and 3 yr), and older ages (age greater than 3 yr). Heritability estimates averaged over breeds …