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Articles 121 - 130 of 130
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The Generation Of Cytotoxic Tumour Infiltrating Lymphocyte Clones Against Head And Neck Squamous Cell Carcinoma, Henry J. Lapointe
The Generation Of Cytotoxic Tumour Infiltrating Lymphocyte Clones Against Head And Neck Squamous Cell Carcinoma, Henry J. Lapointe
Digitized Theses
73 samples of H&NSCCa were processed for these studies. An enzymatic disaggregation protocol for the flow cytometric analysis of DNA content of tumour whole cell preparations was developed. Analysis of the tumour cell component without the need for incorporation of reference controls provided a more accurate assessment of DNA content of tumour cell populations. Six lines of H&NSCCa were established in vitro from fresh tumour samples. Tumour cell effects on PHA- or IL-2 stimulated proliferation of PBMNCs ranged from 61% and 45% suppression to 4% and 121% enhancement of the proliferative responses respectively. Flow cytometric analysis revealed a down regulation …
Structure/Function Studies Of Trypanosome Variant Surface Glycoproteins, Vern Bruce Carruthers
Structure/Function Studies Of Trypanosome Variant Surface Glycoproteins, Vern Bruce Carruthers
Digitized Theses
Antigenic variation is manifested by many prominent infectious organisms in adaptive response to host immunity. However, polymorphism in antigenic structure is often limited by the necessity to maintain functionality of the antigen. It follows that it may be possible to design molecularly defined vaccines capable of accounting for variable antigenic phenotype. For this reason, the focus of the present thesis was to develop a model system using trypanosome variant surface glycoprotein (VSG) for examining the effects of amino acid sequence variation on the specificity of antigenic determinants.;In order to assess the suitability for a model system of four VSGs (isoVAT …
Magnetic Resonance Spectroscopy Of Probe Labelled Glycolipids In Model Membranes, And Development Of Lipid Probes For Magnetic Resonance Imaging, Eugene Florio
Digitized Theses
Glycosphingolipids are the carbohydrate-bearing lipids of animal cells. These usually minor constituents of the plasma membrane have been proposed to have major roles in structure and recognition. Glycosphingolipids are distinctly different from phospholipids in that: (i) they possess donor H-bonding groups, (ii) they often have disproportionately long, single fatty acid chains and (iii) they can be {dollar}\alpha{dollar}-hydroxylated on the fatty acid chain.;As part of a research program to investigate the behaviour and interaction of glycosphingolipids in biological membranes we have developed spin labelled and deuterium labelled derivatives. These included novel 24-carbon (lignoceric acid) spin labelled at C-12 or C-16, and …
Studies On The In Vitro Transposition Of Bacteriophage Mu Dna, Michael Gordon Surette
Studies On The In Vitro Transposition Of Bacteriophage Mu Dna, Michael Gordon Surette
Digitized Theses
The early steps in the transposition of Mu DNA have been examined in vitro using purified Mu A, Mu B and E. coli HU proteins, a mini-Mu donor plasmid and target DNA. Two stable protein-DNA complexes, or transpososomes, have been characterized. Type 1 complex formation requires a supercoiled mini-Mu donor plasmid, Mu A and HU protein. In the Type 1 complex the two ends of Mu are held together, creating two independent topological domains; a supercoiled Mu domain and a relaxed vector domain (due to nicking at the Mu ends). In the presence of Mu B protein, ATP, and target …
Food Limitation And The Adaptive Significance Of Clutch Size In American Coots (Fulica Americana), Todd William Arnold
Food Limitation And The Adaptive Significance Of Clutch Size In American Coots (Fulica Americana), Todd William Arnold
Digitized Theses
For many species of birds, egg formation costs are considered important constraints on timing of breeding, clutch size, and egg size. For American Coots (Fulica americana), body reserves and current food availability are both thought to affect these aspects of reproduction. In order to test the egg formation hypothesis, I conducted numerous observational and manipulative experiments on wild, free-ranging American Coots. Clutch size declined with laying date in five out of six years, contrary to seasonal patterns of food availability in prairie wetlands. Clutch size increased during two of three years in response to supplemental feeding. Laying date was only …
Role Of Metallothionein In Chemical Toxicity Mediated By Reactive Intermediates Generated From Xenobiotic And Oxygen Metabolism, Zacharias Emmanuel Suntres
Role Of Metallothionein In Chemical Toxicity Mediated By Reactive Intermediates Generated From Xenobiotic And Oxygen Metabolism, Zacharias Emmanuel Suntres
Digitized Theses
This research was concerned with the role of metallothionein (MT) in chemical toxicity mediated by reactive intermediates generated from xenobiotic and oxygen metabolism. Metallothionein(s) are ubiquitous, low molecular weight, cysteine-rich (30% of total amino acids), metal binding proteins. These proteins have been shown to be important in the regulation of essential metal metabolism and in the detoxication of toxic metals. In the present research it was hypothesized that (i) because of its high cysteinyl thiolate concentration, MT is reactive towards intermediates generated from xenobiotic metabolism; and (ii) the metal (Zn, Cu) released from MT concomitant with the inactivation of metal …
Growth Temperature Effects On Photosynthesis And Photoinhibition Of Spinach (Spinacia Oleracea L), Steven Ray Boese
Growth Temperature Effects On Photosynthesis And Photoinhibition Of Spinach (Spinacia Oleracea L), Steven Ray Boese
Digitized Theses
Spinacea oleracea L. cv Savoy was utilized to determine the effects of growth temperature and temperature shifts after leaf expansion on growth, photosynthesis and photoinhibition.;Growth was slowed at 5{dollar}\sp\circ{dollar}C with the second leaves reaching full expansion at 92 days after planting compared to 32 days for 16{dollar}\sp\circ{dollar}C grown plants. Growth at 5{dollar}\sp\circ{dollar}C compared to 16{dollar}\sp\circ{dollar}C resulted in a 2 to 3 fold increases in leaf area, dry weight per area, and leaf thickness of fully expanded leaves. Further, growth at 5{dollar}\sp\circ{dollar}C resulted in an increase from 2 to 3 palisade layers and an increase in the freezing tolerance from {dollar}-2\sp\circ{dollar}C …
Natural Lta Tumor Cell Resistance To Malignant Conversion By Oncogenesras Andmyc, Alan Bradley Tuck
Natural Lta Tumor Cell Resistance To Malignant Conversion By Oncogenesras Andmyc, Alan Bradley Tuck
Digitized Theses
A tumorigenic, non-metastatic murine fibroblast cell line (LTA) was here used to study aspects of natural cellular resistance to induction of progression to malignancy. The nature of LTA cell growth was established by testing both in vitro and in vivo. In vitro growth showed some but not all characteristic features of transformation (eg. cells showed little contact inhibition of cell growth and were able to multilayer, but were not serum independent). In vivo tumor growth was that of a benign (non-metastatic) fibrosarcoma. Attempts were made to induce LTA cell progression to malignancy by both epigenetic and genetic means. Treatment of …
Solvent Dependence Of Photoinduced Intramolecular Electron Transfer, Jing-Yao Liu
Solvent Dependence Of Photoinduced Intramolecular Electron Transfer, Jing-Yao Liu
Digitized Theses
This work is a study of the solvent and temperature dependence of photoinduced intramolecular electron transfer (ET). The molecule containing a porphyrin and a quinone linked by a phenylalanine amino acid residue was synthesized. Excitation of this molecule (PPAQ) causes electrons to be transferred from the porphyrin to the quinone entity. The ET rate constants were measured by comparing the porphyrin fluorescence lifetime with or without the possibility of ET.;The ET rate constants of PPAQ were measured for several solvents and compared with data of other similar porphyrin-quinone linked molecules. A temperature dependence of ET rate constants study was also …
Psychological Well-Being: Personality And Social Role Perspectives, Kathryn Ann Dance
Psychological Well-Being: Personality And Social Role Perspectives, Kathryn Ann Dance
Digitized Theses
This thesis was concerned with an examination of psychological well-being, defined as consisting of negative affect, positive affect, and self-esteem. Two distinct approaches to well-being were identified (i.e. personality and social role). It was argued that well-being is likely determined by both personality and social role influences, and that these two areas might themselves be interrelated. Both cognitive and affective personality factors were included in this research (i.e. dysfunctional cognitions and affect intensity). Roles were examined at a general level (number of roles and their cognitive representation in terms of complexity) and at a specific level that included assessments of …