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Laser-Molecule Interactions And The Effects Of Permanent Dipoles, Anne E. Kondo
Laser-Molecule Interactions And The Effects Of Permanent Dipoles, Anne E. Kondo
Digitized Theses
The spectral and dynamic effects associated with a non-zero difference, d, between the permanent dipole moments of two molecular states involved in a transition, are investigated for a variety of electromagnetic field-molecule interactions. Both analytical and exact numerical techniques are used to solve the time-dependent Schrodinger equation and to obtain the temporal populations of the molecular states and the associated absorption spectra, for molecular interactions with a Gaussian pulsed, or one or two continuous wave (CW), laser(s).;Analytical rotating wave approximation results are derived for a single CW laser interacting with a two-level d {dollar}\ne{dollar} 0 molecule with an excited state …
Characterization And Expression Of Polyubiquitin And Ubiquitin-Fusion Protein Genes In Tissues From Control And Heat-Shocked Maize Seedlings, Ling Liu
Digitized Theses
Ubiquitin is a highly conserved, 76 amino acid, heat-stable protein found in many eukaryotic cells, including Zea mays L. Northern and dot blot hybridization analyses, using the ubiquitin coding sequence as a probe, demonstrate that the ubiquitin genes in maize are constitutively expressed at a control temperature (25{dollar}\sp\circ{dollar}C), and that heat shock (42.5{dollar}\sp\circ{dollar}C) induces an increase in polyribosome-associated ubiquitin mRNAs in the radicles (4-fold) and plumules (2-fold) of 5-day-old maize (inbred: Oh43) seedlings. These data imply that maize ubiquitin is a heat shock protein.;To assess the implication that maize ubiquitin is a heat shock protein encoded from genes whose expression …
The Structure, Biosynthesis And Function Of Corticosteroid Binding Globulin, Carolyn L. Smith
The Structure, Biosynthesis And Function Of Corticosteroid Binding Globulin, Carolyn L. Smith
Digitized Theses
Corticosteroid binding globulin (CBG) is the major plasma transport protein for glucocorticoids. Complementary DNAs for rat, mouse, rabbit and squirrel monkey CBG were isolated, sequenced and their deduced primary structures were aligned. Overall, they exhibit 40.9% identity, and their comparison revealed conserved N-glycosylation sites and the probable position of the cysteine located within the human CBG steroid binding domain. Rat CBG mRNA is produced primarily by the liver, and in adults, males have two-thirds the CBG mRNA levels of females. During the last third of pregnancy, maternal hepatic CBG mRNA levels are relatively constant while fetal levels are highest at …
Studies Of Cis- Andtrans-Acting Genetic Elements In The Genome Of Minute Virus Of Mice, Ralph Salvino
Studies Of Cis- Andtrans-Acting Genetic Elements In The Genome Of Minute Virus Of Mice, Ralph Salvino
Digitized Theses
Studies presented in this thesis involve the identification and/or further characterization of cis-acting and trans-acting genetic elements required for minute virus of mice (MVM) DNA replication and gene expression. Plasmids with mutations introduced into the viral genome within the full length infectious plasmid pMM984 were transfected into murine A9 and primate COS-7 cells and viral DNA replication and gene expression were assessed using in vivo transient DNA replication and chloramphenicol acetyl transferase expression assays.;Results indicated that the nonstructural gene product NS-1 is a trans-acting protein that is required for the excision and replication of the viral genome and for capsid …
Studies On The In Vitro Transposition Of Bacteriophage Mu Dna, Michael Gordon Surette
Studies On The In Vitro Transposition Of Bacteriophage Mu Dna, Michael Gordon Surette
Digitized Theses
The early steps in the transposition of Mu DNA have been examined in vitro using purified Mu A, Mu B and E. coli HU proteins, a mini-Mu donor plasmid and target DNA. Two stable protein-DNA complexes, or transpososomes, have been characterized. Type 1 complex formation requires a supercoiled mini-Mu donor plasmid, Mu A and HU protein. In the Type 1 complex the two ends of Mu are held together, creating two independent topological domains; a supercoiled Mu domain and a relaxed vector domain (due to nicking at the Mu ends). In the presence of Mu B protein, ATP, and target …