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Lipopolysaccharide Induced Septicemia In Chronic Morphine Treated Rats, Frank Ocasio
Lipopolysaccharide Induced Septicemia In Chronic Morphine Treated Rats, Frank Ocasio
Seton Hall University Dissertations and Theses (ETDs)
Opiate-addicts have been known to show increased susceptibility to bacterial infection. Lipopolysaccharide (LPS), which is a cell wall component in gram-negative bacterial, is a potent stimulator of inflammation. We investigated how treatment with morphine alters LPS-induced inflammatory responses in the rat. Chronic morphine exposure alone elevated serum endotoxin levels. Animals treated with morphine and LPS (250 µglkg) developed hypothermia, decreased mean arterial pressure (MAP), increased plasma thrombin anti-thrombin III (TAT) complex, and approximately 67% exhibited progressive intramicrovascular coagulation. Morphine also enhanced LPS induced leukocyte endothelial adhesion (LEA), suppressed leukocyte flux and corticosterone production, and elevated interleukin- I�, tumor necrotic factor-a, …
Studies On The Regulation Of Mu Opioid Receptor Mrna Expression In Shsy-5y Human Neuroblastoma Cells, Xin Yu
Studies On The Regulation Of Mu Opioid Receptor Mrna Expression In Shsy-5y Human Neuroblastoma Cells, Xin Yu
Seton Hall University Dissertations and Theses (ETDs)
Prolonged exposure to morphine down-regulates mu opioid receptors (MOR) on both undifferentiated and differentiated (retinoic acid or phorbol ester treated) SHSY-5Y cells. However, morphine pretreatment does not alter MOR receptor affinity for morphine. To investigate the molecular basis for MOR regulation after exposure to its selective agonists, we have developed a quantitative competitive reverse transcriptase-polymerase chain reaction (QC-RT PCR) to quantify the expression of MOR in SHSY-SY cells. Differentiation of SHSY-5Y cells with retinoic acid or phorbol ester up regulated MOR mRNA expression by 30 % and 78%, respectively. A 24 hours treatment with morphine (10 µM) down regulated MOR …
Dara, The Antirestriction Protein Of Bacteriophage P1, Priti R. Vemulapalli
Dara, The Antirestriction Protein Of Bacteriophage P1, Priti R. Vemulapalli
Seton Hall University Dissertations and Theses (ETDs)
No abstract provided.
Regulation Of Proto-Oncogenes Expression In Developmental Epilepsy, Mahnoush Shafiei
Regulation Of Proto-Oncogenes Expression In Developmental Epilepsy, Mahnoush Shafiei
Seton Hall University Dissertations and Theses (ETDs)
Seizures cause selective neuronal cell loss in vulnerable regions such as the hippocampus, cortex, thalamus or substantia nigra (SN) in human or experimental animals. While the HF can propagate seizures, the SN pars reticulata (SNR) regulates the spread ol seizures in an age-dependent fashion, and the pars compacta (SNC) affects extrapyramidal motor control. In mature animals, both structures are wlnerable to seizure-induced damage. In prepubescent rats, seizure susceptibility is reduced and CA 1 region rs primarily affected. The proto-oncogenes, Bcl-2 and Bax, encode specific proteins that inhibit and promote programmed cell death, respectively. In order to determine whether proto-oncogenes are …
Genetic Diversity Of Balbiani Ring L Gene In Two Species Of Chironomus, Lola-Mae Palmer
Genetic Diversity Of Balbiani Ring L Gene In Two Species Of Chironomus, Lola-Mae Palmer
Seton Hall University Dissertations and Theses (ETDs)
Differences in genetic composition have been predicted among aquatic midge fly larva called chironomids. Although several genes including microsatellites and ribosomal protein genes have been explored in our lab, the gene that has so far been studied the most is the Balbiani Ring (BR) 1 gene. There are several genes in this family, including Balbiani Ring 2.1, 2.2 and 6. These BR genes are known to contain long arrays of tandemly repeated units with a ranked repeat organization (Paulsson et al.1992). This secretory protein gene forms a large puff located on polytene chromosome IV (Bentivegna et al., 1993). The gene …
Acute And Persistent Effects Of Interleukin-1ss And Stress On The Hpa Axis, Tara Tumminello
Acute And Persistent Effects Of Interleukin-1ss And Stress On The Hpa Axis, Tara Tumminello
Seton Hall University Dissertations and Theses (ETDs)
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Dipyridamole Reduces Oxidative Stress In Human Coronary Artery Endothelial And Hela Cell Models, Matthew E. Giangrante
Dipyridamole Reduces Oxidative Stress In Human Coronary Artery Endothelial And Hela Cell Models, Matthew E. Giangrante
Seton Hall University Dissertations and Theses (ETDs)
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Formation Of Multistranded Structures From The Self-Assembly Of G-Rich Dna Oligomers By Various Sequence And Environmental Influences, Bryan D. Vi
Seton Hall University Dissertations and Theses (ETDs)
No abstract provided.
Morphine Enhances The Permeability Across Vascular Endothelial Cell Barriers, Jenine Anday
Morphine Enhances The Permeability Across Vascular Endothelial Cell Barriers, Jenine Anday
Seton Hall University Dissertations and Theses (ETDs)
Using three types of in vitro endothelial cell barrier models, we investigated the direct effects and mechanisms of morphine on vascular permeability. Our current studies illustrate LPS induces permeability across these VEC barriers, and is significantly enhanced when co-treated with morphine, displayed using the [ 14 CJ-inulin paracellular marker. Incubation with morphine alone induces permeability in a concentration depeodent manner, and is not blocked by the addition of naloxone. Morphine enhances the detrimental effects of LPS on cell viability and alone also decreases endothelial cell viability, concentration-dependently, which is also not affected by naloxone, as demonstrated by the trypan blue …
Cloning Of Hsds Gene And Characterization Of The Protein From Haemophilus Influenzae Rd, Srividya Chalapatti
Cloning Of Hsds Gene And Characterization Of The Protein From Haemophilus Influenzae Rd, Srividya Chalapatti
Seton Hall University Dissertations and Theses (ETDs)
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Effects Of Antimetabolites On Sclerotium Rolfsii Growth And Oxalic Acid Production, Ezikiel Okafor-Agilo
Effects Of Antimetabolites On Sclerotium Rolfsii Growth And Oxalic Acid Production, Ezikiel Okafor-Agilo
Seton Hall University Dissertations and Theses (ETDs)
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Molecular Taxonomy Of The Lichen Genus Heterodermia, Linda Hirschberger
Molecular Taxonomy Of The Lichen Genus Heterodermia, Linda Hirschberger
Seton Hall University Dissertations and Theses (ETDs)
Heterodermia is lichen consisting of a fungus and algae. Morphology and chemistry cannot provide definitive evidence for the identity of Heterodermia because various species have very similar morphologies that cannot always be distinguished. Other species only differ by the presence or absence of secondary chemicals detected. by specific chemical tests, which makes distinguishing between two species difficult. The objective of this project is to use Restriction Fragment Length Polymorphisms (RFLPs) to determine how closely related each Heterodermia species is to one another. RFLPs will also be used to determine how closely related a species is to its equal in another …
Analysis Of Genetic Factors Affecting High Density Lipoprotein Levels And Apoall Levels In C57 B1/6j And Fvb/N Wildtype Mice Using A Qtl Mapping Approach, Judith Barrios
Seton Hall University Dissertations and Theses (ETDs)
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Studying The Expression Of A Novel Microsatellite Mrna In Chironomous Riparius, Mina Ranzurmal
Studying The Expression Of A Novel Microsatellite Mrna In Chironomous Riparius, Mina Ranzurmal
Seton Hall University Dissertations and Theses (ETDs)
The expression of a unique mRNA containing a microsatellite sequence was studied in C. riparius using differential display. First, the rnicrosatellite fragment was sequenced using Sanger's Sequencing method. The microsatellite cDNA contained 11 "ACA" repeats, 12 "AC" repeats, and 5 "CTC" repeats. Second, the affects of cadmium and heat shock on rnicrosatellite mRNA expression were studied. In cadmium experiments, larvae were exposed to low cadmium concentrations (0, 0.04, 0.4, and 4.0 mM) for 24 and 48 h and high cadmium concentrations (0, 1.0, 2.0, 4.0, 8.0, and 16.0 mM) for 48 h. The effects of cadmium on fed larvae at …
Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr.
Standardization Of An Immunoassay For The Detection Of Antibodies To B2 Glycoprotein-I, Eric N. Erickson Jr.
Seton Hall University Dissertations and Theses (ETDs)
Antiphospholipid Syndrome (APS) is an evolving autoimmune disease with numerous clinical manifestations. APS occurs in two forms: Primary Antiphospholipid Syndrome (PAPS) and secondarily in association with other autoimmune disorders such as Systemic Lupus Erythematosis (SLE). In the brief period since its discovery as a cofactor for anticardiolipin antibodies, p1-glycoprotein I (�GPI; apolipoprotein H) has been recognized as the autoantigen in the absence of anionic phospholipid when appropriately presented to human autoantibodies. Recent studies suggest that this presentation requires the surface-dependent unmasking of a cryptic epitope. Immunoassays of autoantibodies directed against p1GPI, developed through the application of suitable polymeric matrices, may …
Characterization Of The Adenovirus Protease Activity In Adenovirus Gene Therapy Preparations, Frederick William Porter Iv
Characterization Of The Adenovirus Protease Activity In Adenovirus Gene Therapy Preparations, Frederick William Porter Iv
Seton Hall University Dissertations and Theses (ETDs)
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Characterization Of A Membrane Bound Proteolytic Enzyme Of Rat Osteoblasts, Anthony M. Orrico
Characterization Of A Membrane Bound Proteolytic Enzyme Of Rat Osteoblasts, Anthony M. Orrico
Seton Hall University Dissertations and Theses (ETDs)
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Developing A Gene Specific Probe For Phosphoenolpyruvate Carboxykinase Mrna, Stephen M. Kuntz
Developing A Gene Specific Probe For Phosphoenolpyruvate Carboxykinase Mrna, Stephen M. Kuntz
Seton Hall University Dissertations and Theses (ETDs)
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Effects Of Lead On Inflammatory Microhemodynamics In Male Wistar Rats, Tanaz Mistry
Effects Of Lead On Inflammatory Microhemodynamics In Male Wistar Rats, Tanaz Mistry
Seton Hall University Dissertations and Theses (ETDs)
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Partial Sequencing Of A Balbiani Ring Gene And Development Of A Molecular Probe, Kerri Ann Kurzeja
Partial Sequencing Of A Balbiani Ring Gene And Development Of A Molecular Probe, Kerri Ann Kurzeja
Seton Hall University Dissertations and Theses (ETDs)
The Environmental Protection Agency is currently developing sediment quality criteria in order to protect the nation's water sources. In order to assist in the development of standards for the disposal of heavy metals, our laboratory is undergoing sediment toxicity studies on Chironomus riparius. It is an EPA approved test organism that spends most of its life in the sediment. Following a previous study (Cochrane et al., 1994), the organism's response to heavy metals will be monitored on the genetic level. Several genes, including a Balbiani ring (BR) gene, which was the focus of this thesis, will be studied to determine …
Identification Of An Amylase-Like Gene In Chironomus Riparius, Loriann Mahurter
Identification Of An Amylase-Like Gene In Chironomus Riparius, Loriann Mahurter
Seton Hall University Dissertations and Theses (ETDs)
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Binding Of Cobalt (Iii) To Nucleic Acids Via Reaction With [Co(Nh3)5(Oh2)]3+, David M. Calderone
Binding Of Cobalt (Iii) To Nucleic Acids Via Reaction With [Co(Nh3)5(Oh2)]3+, David M. Calderone
Seton Hall University Dissertations and Theses (ETDs)
No abstract provided.