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Seton Hall University Dissertations and Theses (ETDs)

Theses/Dissertations

Cyanobacteria

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Detection, Identification, And Metagenomic Characterization Of Cyanobacteria In Barnegat Bay, New Jersey, Roksana M. Rahman May 2019

Detection, Identification, And Metagenomic Characterization Of Cyanobacteria In Barnegat Bay, New Jersey, Roksana M. Rahman

Seton Hall University Dissertations and Theses (ETDs)

Cyanobacterial harmful algal blooms (CHAB), caused by eutrophication, is known to threaten both wildlife and human health. Barnegat Bay, also home to Oyster Creek nuclear power plant, has experienced an elevated levels of nitrogen due to human activity such as recreational activity and urbanization. This has caused an increase of CHAB’s at a more frequent rate within Barnegat Bay. In order to detect possible CHAB causing cyanobacteria, water samples were collected from 12 different locations within Barnegat Bay. Each sample was filtered through a 30- and 0.4-mm polycarbonate filter sequentially. Chelex DNA extraction, polymerase chain reaction (PCR), and gel electrophoresis …


Flow Cytometric Analysis For Cyanobacteria In 36 New Jersey Freshwater Bodies, Ruchit Patel May 2016

Flow Cytometric Analysis For Cyanobacteria In 36 New Jersey Freshwater Bodies, Ruchit Patel

Seton Hall University Dissertations and Theses (ETDs)

Eutrophication, a process that occurs due to excessive accumulation of nutrients such as nitrogen and phosphorous is primarily from anthropogenic nitrogen and natural occurrences. This phenomenon causes cyanobacterial overgrowth which can potentially lead to toxic algal blooms that affect public drinking water sources and recreational usage. An immediate need to detect bloom forming cyanobacteria in freshwater bodies early hand is critical to implement prevention strategies. These microorganisms contain phycobiliproteins such as phycoerytrhin, and allophycocyanin as part of the phycobillisome that allow autofluorescence. In this study, 36 freshwater bodies from 14 New Jersey counties were collected and processed for flow cytometric …


Identification And Characterization Of Phytoplankton In Barnegat Bay, New Jersey, Nicole L. Elia May 2014

Identification And Characterization Of Phytoplankton In Barnegat Bay, New Jersey, Nicole L. Elia

Seton Hall University Dissertations and Theses (ETDs)

Barnegat Bay is a marine estuary that encompasses 670-square miles of the eastern coast of New Jersey. The bay serves as a home to thousands of species of plants, animals, and microorganisms. During the summer months, the population of the bay increases from 576,000 to over 1.5 million residents. In 2011, the Barnegat Bay Partnership reported that environmental conditions of the bay are declining due to increased pollutants from nutrient fertilizers and runoff. Increased nitrogen levels have led to eutrophication and proliferation of phytoplankton algal blooms. Harmful algal blooms (HABs) cause a decrease in dissolved oxygen levels in the water …