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Effects Of Sodium Orthovanadate On Lps-Induced Senescent Raw 264.7 Cells, Andrew Pugliese
Effects Of Sodium Orthovanadate On Lps-Induced Senescent Raw 264.7 Cells, Andrew Pugliese
Seton Hall University Dissertations and Theses (ETDs)
Cellular senescence is considered a signal transduction process where damaged or stressed cells arrest proliferation. Exogenous stressors including bacterial infection, chemotherapy, and irradiation can drive normal cells to senescence. Sodium orthovanadate (Na3VO4) is a phosphatase inhibitor known to mediate the release of the proinflammatory cytokine, TNF-a. We hypothesized that NaVO4 treatment will reduce proinflammatory expression from senescent cells. In our study we illustrate that prolonged exposure to LPS caused RAW 264.7 cells induced morphological changes indicative of senescent cells including the formation of giant multinucleated cells (GMCs). Furthermore, we found that RAW 264.7 GMC cells …
Effects Of Palmitate On The Expression Of Inflammatory Cytokines In Microglial Cells, Shiyu Ma
Effects Of Palmitate On The Expression Of Inflammatory Cytokines In Microglial Cells, Shiyu Ma
Seton Hall University Dissertations and Theses (ETDs)
Obesity has become a pandemic health issue for the development of more serious illnesses such as insulin resistance, cardiovascular diseases, and hypertension. Among those pathological conditions, low grade chronic inflammation triggered by saturated fatty acids (SFA) such as palmitate (PA) has been suggested to be the culprit of crimes and various signaling modulators ranging from cellular surface receptors such as toll like receptors (TLRs) and G- protein – coupled receptor 40 (GPR40) to intracellular kinases like mitogen-activated protein kinase (MAPK) and protein kinase C (PKC) have been postulated. In this study, mouse BV2 cells were treated with different concentrations of …
Cd11b+ Depletion Of Bone Marrow Derived Macrophages Negatively Affects Bone Fracture Healing, Sarah Hozain
Cd11b+ Depletion Of Bone Marrow Derived Macrophages Negatively Affects Bone Fracture Healing, Sarah Hozain
Seton Hall University Dissertations and Theses (ETDs)
Hematoma formation and inflammation occurs during the beginning stages of fracture repair, which require the presence of innate cells such as macrophages. Macrophages are subdivided into proinflammatory, M1, or anti-inflammatory, M2, phenotypes with different functions, cytokine profiles, and surface markers. In this study, in vitro and in vivo models were used to deplete M1 macrophages, using Mac-1 Sap conjugated antibody (Mac1SAP), to determine the effects on fracture healing. In vitro study, primary macrophages isolated from mice femoral bone marrow were harvested and differentiated into M1 macrophages (+LPS), M2 macrophages (+IL-4), or undifferentiated then treated with either vehicle or 10pM Mac1SAP. …
Endotoxin-Induced Inflammation & Morphine-Induced Conditioned Place Preference In The Hiv-1 Transgenic Rat, Natasha F. Homji-Mishra
Endotoxin-Induced Inflammation & Morphine-Induced Conditioned Place Preference In The Hiv-1 Transgenic Rat, Natasha F. Homji-Mishra
Seton Hall University Dissertations and Theses (ETDs)
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