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Effects Of Palmitate On The Expression Of Inflammatory Cytokines In Microglial Cells, Shiyu Ma
Effects Of Palmitate On The Expression Of Inflammatory Cytokines In Microglial Cells, Shiyu Ma
Seton Hall University Dissertations and Theses (ETDs)
Obesity has become a pandemic health issue for the development of more serious illnesses such as insulin resistance, cardiovascular diseases, and hypertension. Among those pathological conditions, low grade chronic inflammation triggered by saturated fatty acids (SFA) such as palmitate (PA) has been suggested to be the culprit of crimes and various signaling modulators ranging from cellular surface receptors such as toll like receptors (TLRs) and G- protein – coupled receptor 40 (GPR40) to intracellular kinases like mitogen-activated protein kinase (MAPK) and protein kinase C (PKC) have been postulated. In this study, mouse BV2 cells were treated with different concentrations of …
The Effects Of Palmitate On The Expression Of G-Protein Coupled Receptors And Glut Transporters In Neuro2a Cells, Brian Montoya
The Effects Of Palmitate On The Expression Of G-Protein Coupled Receptors And Glut Transporters In Neuro2a Cells, Brian Montoya
Seton Hall University Dissertations and Theses (ETDs)
Palmitate (PA) is a saturated fatty acid that has been reported to be elevated in individuals with metabolic syndromes. The effects of fatty acids on the expression of various fatty acid receptors on neuronal cells is unknown, as is the effects of fatty acids on GLUT transporters. In this study, Neuro-2a (N2a) cells were treated with either 300μM of bovine serum albumin (BSA) or 300μM of palmitate conjugated to BSA (PA) at various time points to determine the effect of palmitate on the expression of various fatty acid receptors and GLUT transporters. RNA isolation followed by RT-PCR with primers specific …
Investigations On The Effects Of Palmitate On Neuronal Cells, Ryan Gelsinger
Investigations On The Effects Of Palmitate On Neuronal Cells, Ryan Gelsinger
Seton Hall University Dissertations and Theses (ETDs)
Many studies have demonstrated that increased levels of free fatty acids (FFAs) are associated with increased apoptosis in hepatocytes, podocytes, myocytes, and pancreatic islet cells. However, the effects of FAAs on neuronal cell function are not well characterized. In this study, mouse neuroblastoma cells from the Neuro-2a (N2a) cell line were treated with either bovine serum albumin (BSA) or different concentrations of BSA-conjugated palmitate (PA) and examined cell viability using MTT assay. Concentrations of PA at or above 200 μM in the growth media were associated with a decrease in cell viability. In order to examine whether N-acetylcysteine (NAC) or …