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Metabarcoding-Biomass Relationships And Internal Standards For Quantitative Dna Metabarcoding, Frances Webber 2025 University of Rhode Island

Metabarcoding-Biomass Relationships And Internal Standards For Quantitative Dna Metabarcoding, Frances Webber

Open Access Master's Theses

DNA metabarcoding has been widely adopted due to its power in investigating the composition of protistan communities, accurately identifying even small and cryptic taxa. However, the compositional nature of DNA metabarcoding results, and the distortion of natural abundances due to variation of the often-used 18S rRNA gene copy number over several orders of magnitude, renders these results semi-quantitative at best, limiting the application of DNA metabarcoding to ecological study. Here, we constructed mock communities from uni-algal cultures of taxonomically diverse phytoplankton and conducted metabarcoding using multiple primer sets and both DNA and cDNA as PCR templates. For each culture, chlorophyll …


Analyzing Seasonal Wind Trends And Climate Change Impacts Along The East Coast Of The United States (1990-2022), Avery L. Wilson 2025 University of New Hampshire, Durham

Analyzing Seasonal Wind Trends And Climate Change Impacts Along The East Coast Of The United States (1990-2022), Avery L. Wilson

Honors Theses and Capstones

This study investigates long-term trends in ocean wind intensity and variability along the U.S. East Coast between 1990 and 2022 using ECMWF ERA5 reanalysis data. Focusing on monthly maximum, mean, and standard deviation (STD) of wind speed at 10 meters above the surface, linear regressions were performed to analyze long-term trends. Results indicate a significant increase in wind intensity and variability in the northeastern region, particularly during winter months, which may forecast more frequent and intense cyclonic activity such as Nor’easters. Conversely, a weak decreasing trend was observed in the south. These shifts have implications for ocean-atmosphere dynamics, including vertical …


A Socio-Ecological Investigation Of Ulithian Spearfishing, Joshua Pi 2025 University of Rhode Island

A Socio-Ecological Investigation Of Ulithian Spearfishing, Joshua Pi

Open Access Dissertations

Coral reef socio-ecological systems (SESs) characterize the interconnected relationships between coral reef ecosystems and reef-dependent communities. One such SES exists in Ulithi Atoll, Federated States of Micronesia, where Ulithians fish, steward, and govern their reefs through customary marine tenure, traditional ecological knowledge, and cultural taboos. In recent decades, rapid modernization in Ulithi has introduced new fishing technologies, transformed fishing practices, and ultimately reshaped the functioning of Ulithi’s traditional SES. One significant change was the introduction of spearfishing during the mid-20th century, which is more selective and individualistic compared to other community-centric fishing methods that have existed for generations. This …


Comparing Surface Currents Near The Mouth Of Three Bays Along The U.S. East Coast: Chesapeake Bay, Delaware Bay, And New York Bay, Tal Ezer, Teresa Updyke 2025 Old Dominion University

Comparing Surface Currents Near The Mouth Of Three Bays Along The U.S. East Coast: Chesapeake Bay, Delaware Bay, And New York Bay, Tal Ezer, Teresa Updyke

CCPO Publications

Monthly surface currents at 2 km resolution near the mouths of three U.S. east coast bays were obtained from high-frequency radars (Coastal Ocean Dynamics Application Radar, CODAR) during 2012–2024. The currents near these bays, the Chesapeake Bay (CB), the Delaware Bay (DB) and the New York Bay (NB) were analyzed to infer similarity and differences, as well as potential common forcing from regional and basin-scale factors. The contribution to flow variability from local and remote forcing is evaluated by comparing surface currents with (a) river discharges into each bay, (b) with local and regional winds, and (c) with the North …


Multi-Model Estimate Of Antarctic Ice-Shelf Basal Mass Budget And Ocean Drivers, Benjamin K. Galton-Fenzi, Richard Porter-Smith, Sue Cook, Eva Cougnon, David E. Gwyther, Wilma G. C. Huneke, Madelaine G. Rosevear, Xylar Asay-Davis, Fabio Boeira Dias, Michael S. Dinniman, David Holland, Kazuya Kusahara, Kaitlin A. Naughten, Keith W. Nicholls, Charles Pelletier, Ole Richter, Hélène Seroussi, Ralph Timmerman 2025 Australian Antarctic Division

Multi-Model Estimate Of Antarctic Ice-Shelf Basal Mass Budget And Ocean Drivers, Benjamin K. Galton-Fenzi, Richard Porter-Smith, Sue Cook, Eva Cougnon, David E. Gwyther, Wilma G. C. Huneke, Madelaine G. Rosevear, Xylar Asay-Davis, Fabio Boeira Dias, Michael S. Dinniman, David Holland, Kazuya Kusahara, Kaitlin A. Naughten, Keith W. Nicholls, Charles Pelletier, Ole Richter, Hélène Seroussi, Ralph Timmerman

CCPO Publications

Societal adaptation to rising sea levels requires robust projections of the Antarctic Ice Sheet's retreat, particularly due to ocean-driven basal melting of its fringing ice shelves. Recent advances in ocean models that simulate ice-shelf melting offer an opportunity to reduce uncertainties in ice–ocean interactions. Here, we compare several community-contributed, circum-Antarctic ocean simulations to highlight inter-model differences, evaluate agreement with satellite-derived melt rates, and examine underlying physical processes. All but one simulation use a melting formulation depending on both thermal driving (T) and friction velocity (u), which together represent the thermal and ocean current forcings at the …


Using Post-Supercritical Co2-Assisted Method To Deposit Niep Alloy Coating On The Micro-Arc Oxidation Layer Of Az31 Magnesium Alloy And Its Corrosion Behavior Analysis, Kang-Yu Liao, Hung-Hua Sheu, Ming-Yuan Lin, Ming-Chih Kuo, Po-Hsun Wu, Hung-Bin Lee 2025 Department of Mechanical Engineering, Lunghwa University of Science and Technology, Guishan, Taoyuan 335, Taiwan

Using Post-Supercritical Co2-Assisted Method To Deposit Niep Alloy Coating On The Micro-Arc Oxidation Layer Of Az31 Magnesium Alloy And Its Corrosion Behavior Analysis, Kang-Yu Liao, Hung-Hua Sheu, Ming-Yuan Lin, Ming-Chih Kuo, Po-Hsun Wu, Hung-Bin Lee

Journal of Marine Science and Technology–Taiwan

In this study, a Ni-P alloy coating was deposited on the micro arc oxidation (MAO) layer of AZ31 magnesium alloy to improve its corrosion resistance. The MAO layers were prepared under different operated voltages from 300 to 400 V. The results indicated that the optimize operated parameters for MAO layer is at 350 V, the MAO coatings had the most compact microstructure and superior corrosion resistance. In order to improve the corrosion resistance of MAO coating, this study chose to deposit Ni-P alloy coating on the surface of MAO coating via the post supercritical CO2-assisted electroless plating (PSC-CO …


The Effect Of Feedback On A Remote Operator’S Maneuvering Control For Mass Remote Navigation Competency, Hyoseon Hwang, Ik-Hyun Youn 2025 Graduate school of Mokpo National Maritime University, Mokpo, 58628, Republic of Korea

The Effect Of Feedback On A Remote Operator’S Maneuvering Control For Mass Remote Navigation Competency, Hyoseon Hwang, Ik-Hyun Youn

Journal of Marine Science and Technology–Taiwan

The rapid advancement of Maritime Autonomous Surface Ships (MASS) technology has highlighted the growing need for Remote Operators (ROs) to manage MASS effectively. ROs possess specialized competencies in areas such as situational awareness, crisis management, and system operation. Despite the introduction of competency-based training and assessment in the maritime sector through the international convention on standards of training, certification, and watchkeeping for seafarers convention, the framework's implementation remains incomplete, particularly in remote operator training for MASS. This study is purpose to identify which competencies improve rapidly with performance feedback and which improve slowly. This study conducted an experiment to quantitatively …


Evaluation Of Carbon Sequestration In Seagrass Meadows Of Kenting: A Comparative Study With Penghu And Dongsha Island, Zih-Wei Tang, Huei-Fen Chen, Jordi Mahardika Puntu, Ping-Yu Chang, Li Lo, Jian-Jhih Chen, Wen-Chen Chou, Lan-Feng Fan, Ying-Ju Chang 2025 Institute of Earth Sciences, National Taiwan Ocean University, Keelung, Taiwan

Evaluation Of Carbon Sequestration In Seagrass Meadows Of Kenting: A Comparative Study With Penghu And Dongsha Island, Zih-Wei Tang, Huei-Fen Chen, Jordi Mahardika Puntu, Ping-Yu Chang, Li Lo, Jian-Jhih Chen, Wen-Chen Chou, Lan-Feng Fan, Ying-Ju Chang

Journal of Marine Science and Technology–Taiwan

This study investigates the controlling factors of carbon sequestration (CS) in seagrass bed sediments across three distinct environments in Taiwan: the Kenting (KT) coastal area, the Penghu (PH) open inner bay, and the Dongsha (DS) Atoll lagoon. Actual measurements of the substrate depth beneath the sediment and ground-penetrating radar (GPR) scans of the beach reveal that the coral reef bedrock in KT slopes landward. A field survey of the seagrass area in KT was conducted, and three cross-sectional profiles were analyzed to determine their topography, yielding a median sediment thickness of 88.2cm. Based on these measurements, the sediment volume was …


Calculation Of Aquifer Transmissivity From Borehole Resistance Measurements And Pumping Test Results, Fen-Rong Yang, Chien-Wei Huang, Tien-Kuen Huang 2025 Doctor Candidate, Department of Civil Engineering, National Chung-Hsing University, 145 Xingda Rd., Taichung 402, Taiwan

Calculation Of Aquifer Transmissivity From Borehole Resistance Measurements And Pumping Test Results, Fen-Rong Yang, Chien-Wei Huang, Tien-Kuen Huang

Journal of Marine Science and Technology–Taiwan

Transmissivity is a crucial hydrogeological parameter for evaluating groundwater resources. This parameter is conventionally evaluated using pumping tests; however, these tests are costly and can be conducted at few sites. Many researchers have used ground resistance and pumping test data to estimate various hydrogeological parameters. In this study, borehole resistance survey and pumping test data collected by the Taiwan Groundwater Monitoring Network were used to establish the relationship between transmissivity and formation factor for aquifers in the Zhuoshui River alluvial fan. This relationship was then used to estimate the water output of the aforementioned aquifers in a cost-effective manner from …


A Neural Network-Based Sliding Mode Adaptive Control System For Unmanned Surface Vehicles, Sun Qiaomei, An Liang, Jiang Linglin 2025 Jiangsu Shipping College, Nantong, China.

A Neural Network-Based Sliding Mode Adaptive Control System For Unmanned Surface Vehicles, Sun Qiaomei, An Liang, Jiang Linglin

Journal of Marine Science and Technology–Taiwan

Over the past decades, numerous studies have explored the use of autonomous unmanned surface vehicles (USVs). Successful USV operation requires a robust tracking control system to perform a range of tasks. However, USV dynamics are uncertain, time-varying, and nonlinear. This paper presents a neural network-based sliding mode adaptive control (NNSMAC) system to enhance USV tracking performance. Trajectories were generated using spline and polynomial interpolation based on a set of N predefined waypoints. Simulation results show that the proposed method outperforms traditional sliding mode control in error reduction, enabling more effective tracking.


The First Iucn Red List Of Cold-Water Corals Highlights Global Declines, Julia D. Sigwart, A. Louise Allcock, Renata Carolina Mikosz Arantes, Kelsey Archer Barnhill, Narissa Bax, Julia S. Beneti, Saskia Brix, Gudmundur Gudmundsson, Catherine S. McFadden, Severin A. Korfhage, Christi Linardich, Declan Morrissey, Pedro de Oliveira Nascimento, Bárbara de Moura Neves, Steinunn H. Ólafsdóttir, Stefán Ragnarsson, Kaveh Samimi-Namin, Íris Sampaio, Mark E. de Wilt 2025 Senckenberg Research Institute and Natural History Museum

The First Iucn Red List Of Cold-Water Corals Highlights Global Declines, Julia D. Sigwart, A. Louise Allcock, Renata Carolina Mikosz Arantes, Kelsey Archer Barnhill, Narissa Bax, Julia S. Beneti, Saskia Brix, Gudmundur Gudmundsson, Catherine S. Mcfadden, Severin A. Korfhage, Christi Linardich, Declan Morrissey, Pedro De Oliveira Nascimento, Bárbara De Moura Neves, Steinunn H. Ólafsdóttir, Stefán Ragnarsson, Kaveh Samimi-Namin, Íris Sampaio, Mark E. De Wilt

Biological Sciences Faculty Publications

The most well-known species-based conservation tool is the International Union for the Conservation of Nature (IUCN) Red List of Threatened Species. The current coverage of species in the Red List is known to under-represent benthic marine species. Cold-water corals (CWCs) are increasingly recognised as key to deep-water biodiversity and integral to protected vulnerable marine ecosystems (VMEs), but no deep-sea coral species were previously included in the Red List. We selected 22 cold-water coral species in the Northeast Atlantic, including 4 reef-forming stony corals and 18 octocorals including sea pens and gorgonians, and completed the first IUCN Red List global assessments …


A Blueprint To Greener Shorelines: Advancing The Effectiveness, Sustainability, And Widespread Adoption Of Coastal Nature-Based Solutions Through Transdisciplinary Research, Taylor M. Sloey, Sierra Hildebrandt, Rebecca L. Morris, Matthew V. Bilskie, Aaron Bland, David Bushek, Gabriella DiPetto, Daniel Elefant, Vincent Encomio, Ramin Familkhalili, Christine Hladik, Danielle Kreeger, Avery B. Paxton, Cindy M. Palinkas, LaTina Steele, Andrew Scheld, Daisuke Taira, Jason D. Toft, Armando J. Ubeda, Christine Whitcraft, Donna Marie Bilkovic 2025 Old Dominion University

A Blueprint To Greener Shorelines: Advancing The Effectiveness, Sustainability, And Widespread Adoption Of Coastal Nature-Based Solutions Through Transdisciplinary Research, Taylor M. Sloey, Sierra Hildebrandt, Rebecca L. Morris, Matthew V. Bilskie, Aaron Bland, David Bushek, Gabriella Dipetto, Daniel Elefant, Vincent Encomio, Ramin Familkhalili, Christine Hladik, Danielle Kreeger, Avery B. Paxton, Cindy M. Palinkas, Latina Steele, Andrew Scheld, Daisuke Taira, Jason D. Toft, Armando J. Ubeda, Christine Whitcraft, Donna Marie Bilkovic

Biological Sciences Faculty Publications

Coastal nature-based solutions (NbS) have emerged as powerful tools to enhance sustainable development and ecological restoration goals. As a rapidly growing field spanning across social, political, ecological, economic, and engineering disciplines, it is critical that researchers working in coastal NbS regularly attempt to identify emerging focal areas for scientific inquiry. Following the 27th Biennial meeting of the Coastal and Estuarine Research Federation, we provide a transdisciplinary perspective (including biologists, engineers, oceanographers, geoscientists, economists, and facilitators of workforce training programs) of pertinent research questions that, if answered, will advance the effectiveness, sustainability, and widespread adoption of coastal NbS. These suggestions for …


An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan 2025 Cal Poly Humboldt

An Economic And Production Analysis Of Seaweed Farms In California And The Pacific Northwest, Brian Donovan

Cal Poly Humboldt theses and projects

Since the early 2010s, the seaweed aquaculture industry has steadily expanded in the United States, with states like Alaska, Maine, Hawaii, and Connecticut leading the way in the development of this emerging sector. However, information gaps remain concerning certain aspects of the domestic seaweed aquaculture industry. The purpose of this study was to examine the status of the seaweed aquaculture industry along the West Coast of the United States; specifically in the states of California, Oregon, and Washington. Seaweed farmers in these states were invited to voluntarily complete surveys asking important production, economic, and commercial questions regarding their operations. Data …


Foreign Object Detection System Development Using Edge Devices Integrated With Quadcopter Autonomous Tracking, Chi-Chia Huang, Ching-Hung Lee 2025 Institute of Electrical and Control Engineering, National Yang Ming Chiao Tung University, Taiwan

Foreign Object Detection System Development Using Edge Devices Integrated With Quadcopter Autonomous Tracking, Chi-Chia Huang, Ching-Hung Lee

Journal of Marine Science and Technology–Taiwan

This study proposes an all-weather autonomous foreign-object detection system capable of operating in low-light environments. We used a highly mobile quadcopter equipped with an edge-computing device as the inspection platform. On the software side, we integrated a low-light image enhancement model, a path-tracking algorithm, and a foreign object detection model to achieve rapid object detection. A lightweight, low-light image-enhancement model reduces the need for additional lighting equipment, thereby enabling reliable detection under low-light conditions. The path-tracking algorithm integrates a lightweight semantic segmentation model with traditional image-processing techniques to establish flight paths efficiently and reliably. The foreign-object detection component employs state-of-the-art …


Optimization Of Underactuated Ship Sliding Mode Controller Based On The Ddpg Algorithm, Lin Ma, Mingzhe Qi, Shicai Chen, Hongwei Bian, Jian Zhang 2025 Navigation College, Dalian Maritime University, Dalian 116026, Liaoning, China

Optimization Of Underactuated Ship Sliding Mode Controller Based On The Ddpg Algorithm, Lin Ma, Mingzhe Qi, Shicai Chen, Hongwei Bian, Jian Zhang

Journal of Marine Science and Technology–Taiwan

Due to the development of ship intelligence, the demand for precise controllers is increasing day by day. In traditional design, the trial-and-error method often leads to the use of inaccurate controller parameters, causing oscillations when environmental disturbances are present. To address these issues, this study introduces deep learning to optimize the controller parameters, particularly those used for sliding mode controllers in underactuated ships. We adopt the Deep Deterministic Policy Gradient (DDPG) algorithm based on the Actor Critic framework. By freely setting the reward function to match the control objective, the trained model can output more accurate controller parameters, with the …


Experiments On Aluminum Alloy Additive Layer Waam Lamination Using The Arc Current Reduction Technique, Jukkapun Greebmalai, Eakkachai Warinsiriruk, Chao-Hsiang Hsiao, Yin-Tien Wang 2025 Department of Industrial Engineering, Mahidol University, Nakhon Pathom 73170, Bangkok, Thailand

Experiments On Aluminum Alloy Additive Layer Waam Lamination Using The Arc Current Reduction Technique, Jukkapun Greebmalai, Eakkachai Warinsiriruk, Chao-Hsiang Hsiao, Yin-Tien Wang

Journal of Marine Science and Technology–Taiwan

Recent advancements in additive manufacturing have been propelled by innovations in processes, materials, and fabrication techniques. This study specifically focuses on the optimizing Wire and Arc Additive Manufacturing (WAAM) within the Gas Metal Arc Welding (GMAW) process. The goal is to build laminated layers using aluminum alloy from the five-thousand series. In this research, a novel technique is introduced that employs a reduced arc-current during the additive layer progress for aluminum alloys, along with a double pulse current in the GMAW process to enhance weldability. The material used for addition is ER5356 aluminum wire, which is applied to an Al-5083 …


An Optimization Model For Supporting Bunkering Decisions In Bulk Shipping, Chung-Cheng Lu, Hui-Chieh Li, Hung-Yu Chen 2025 Department of Transportation and Logistics Management, National Yang Ming Chiao Tung University, Taiwan

An Optimization Model For Supporting Bunkering Decisions In Bulk Shipping, Chung-Cheng Lu, Hui-Chieh Li, Hung-Yu Chen

Journal of Marine Science and Technology–Taiwan

This study addresses a bunkering optimization problem for bulk shipping carriers that explores route deviation to find optimal bunkering locations and amounts. A mixed integer linear programming (MILP) model is developed to determine optimal bunkering decisions that minimize total operating costs, including total daily operating costs, bunkering costs, port charges and surcharges, while observing time window constraints at loading/unloading ports. The proposed model is evaluated using instances generated from real data provided by H company with its operational headquarters in Taipei, Taiwan, which operates a fleet of handysize ships in East and South Asia. The results indicate that optimization models …


Assessment Of Coastal Navigation Safety: A Case Study Of Boka Bay With Stakeholder Perspectives, Ivan Mraković, Rino Bošnjak 2025 Faculty of Maritime Studies Kotor, University of Montenegro, Kotor, Montenegro

Assessment Of Coastal Navigation Safety: A Case Study Of Boka Bay With Stakeholder Perspectives, Ivan Mraković, Rino Bošnjak

Journal of Marine Science and Technology–Taiwan

The navigational safety of Boka Bay, i.e., a critical maritime area along the Adriatic coast, is of great importance owing to its ecological sensitivity, diverse marine traffic, and significant economic activity. This study provides a comprehensive assessment by analyzing the perspectives of various stakeholders, including seafarers, local pilots, skippers, regulatory authorities, and others engaged in maritime activities within the bay. Using a structured questionnaire survey, both quantitative and qualitative data were collected in formats such as descriptive responses, Yes/No choices, and Likert scale ratings, ensuring a well-rounded understanding of navigational practices and challenges. The data were analyzed using descriptive statistics, …


A Hybrid Model To Assess Commercial Port Resilience In Taiwan, Po-Hsing Tseng, James J.H. Liou 2025 Department of Shipping & Transportation Management, National Taiwan Ocean University, No.2, Beining Rd., Keelung 202, Taiwan;

A Hybrid Model To Assess Commercial Port Resilience In Taiwan, Po-Hsing Tseng, James J.H. Liou

Journal of Marine Science and Technology–Taiwan

Since 2021, international commercial ports have faced unprecedented challenges (e.g., COVID-19 pandemic, Suez Canal congestion), impacting daily life, work, human well-being, properties, the environment, and socio-economic activities. The adaptation capability resilience of international commercial ports to respond to external changes has become crucial. This study develops an expert knowledge-based hybrid multi-criteria decision-making model integrating Best-Worst Method (BWM), Rough Dombi Aggregator, and Combined Compromise Solution (CoCoSo) to evaluate port resilience capabilities, using Taiwan's international commercial ports as a case study. We propose four evaluation dimensions encompassing 13 indicators: detection capability, resistance capability, resource integration capability, and recovery capability. The BWM determined …


An Evaluation Of The Possibility And Effectiveness Of Renewable Energy In Container Terminal At Jinhae New Port, Min-seop Sim, Yul-seong Kim, Chang-hee Lee 2025 Department of Convergence Interdisciplinary Education of Maritime & Ocean Contents (Logistics System), National Korea Maritime and Ocean University, Busan, Republic of Korea

An Evaluation Of The Possibility And Effectiveness Of Renewable Energy In Container Terminal At Jinhae New Port, Min-Seop Sim, Yul-Seong Kim, Chang-Hee Lee

Journal of Marine Science and Technology–Taiwan

The international goals of combating climate change and reducing greenhouse gas emissions are driving container terminal operators to adopt renewable energy solutions. This study evaluated the feasibility and effectiveness of introducing renewable energy and eco-fuels for energy independence at the Jinhae New Port Container Terminal, South Korea. Using portfolio analysis, eight renewable energy technologies (solar power, solar thermal energy, wind energy, hydropower, marine energy, geothermal energy, bioenergy, and waste-to-energy) and seven eco-friendly fuels (electricity, liquefied natural gas, liquefied petroleum gas, hydrogen fuel cells, ammonia fuel cells, bioenergy, and hybrid systems) were assessed. Survey data from stakeholders identified solar, wind, and …


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