Effect Of Buck Existence On Some Reproductive Hormone Levels During Premating Inakkeçi Goats,
2021
TÜBİTAK
Effect Of Buck Existence On Some Reproductive Hormone Levels During Premating Inakkeçi Goats, Ali̇ Şenok, Erkan Pehli̇van, Fati̇n Cedden
Turkish Journal of Veterinary & Animal Sciences
In this study, the effect of buck presence on serum progesterone, estrogen and FSH levels during the premating in 20 female Akkeçi goats was investigated. The goats were assigned to two groups as follows: control group (n = 10) and treatment group (n = 10). At the beginning of study, first blood samples (day 0) were taken from each goat kept isolated away from bucks at mating season. At day 13, female goats in treatment group were placed in a pen next to, that of bucks in order to provide buck effect. Second blood samples were taken from each does …
Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model,
2021
Faculty of Veterinary Science
Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model, Prasert Apiwatsiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lactic acid bacteria (LAB) have been widely used as probiotics in the livestock industry because of their high potential for antibacterial activity, anticonjugation, antibiofilm capacity, and gut microbiome regulation. Lactobacillus plantarum 22F, 25F (L22F and L25F), and Pediococcus acidilactici 72N (P72N) showed several promising in vitro and in vivo properties, according to our recent research. The objective of this study was to evaluate the Thai LAB efficacy on anticonjugation and antibiofilm activities in E. coli harboring the mcr-1 gene. When compared to the neutralizing cell-free supernatant (CFS), we discovered that the CFS derived from our LAB strains at 1:16 dilution …
Molecular Detection And Genetic Diversity Of Rotavirus A In Domestic Dogs And Cats In Bangkok, Thailand,
2021
Faculty of Veterinary Science
Molecular Detection And Genetic Diversity Of Rotavirus A In Domestic Dogs And Cats In Bangkok, Thailand, Ekkapat Chamsai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Rotavirus A (RVA) in one of the important pathogens causing gastroenteritis in humans and many animal species. The zoonotic potential of RVA has been reported and raises major concerns, especially in high animal-human interfaces settings. The objectives of this thesis were to determine the occurrence of RVAs in dogs and cats, and to investigate the genetic diversity and genetic relationship among RVAs in dogs, cats, human, and other animal species. The total of 572 rectal swab samples were collected from dogs and cats in animal hospitals, in Bangkok and vicinities, during January 2020 to June 2021. The conventional one-step RT-PCR …
In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells,
2021
Faculty of Veterinary Science
In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells, Quynh Le Dang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine mesenchymal stem cells (cMSCs) have potential applications for regenerative therapy, including the generation of insulin-producing cells (IPCs) for studying and treating diabetes. In this study, we established a useful protocol for generating IPCs from canine adipose mesenchymal stem cells (cAD-MSCs). Subsequently, in vitro preservation of pluronic F127-coated alginate (ALGPA)-encapsulated cAD-MSC-derived IPCs was performed to verify ready-to-use (RTU) IPCs. IPCs were induced from cAD-MSCs with the modulated three-stepwise protocol. The first step of definitive endoderm (DE) induction showed that the cooperation of Chir99021 and Activin A created the effective production of Sox17-expressed DE cells. The second step for pancreatic endocrine …
Active And Passive Immune Responses And Protection Of Nile Tilapia (Oreochromis Niloticus) Immunized With Tilapia Lake Virus Inactivated Vaccines,
2021
Faculty of Veterinary Science
Active And Passive Immune Responses And Protection Of Nile Tilapia (Oreochromis Niloticus) Immunized With Tilapia Lake Virus Inactivated Vaccines, Thao Thu Mai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tilapia tilapinevirus, also known as tilapia lake virus (TiLV), is a contagious viral pathogen, resulting in mass mortalities and economic losses for tilapia industry. Here, we developed two simple cell-culture, heat-killed and formalin-killed vaccines (HKV and FKV) aiming to prevent this disease. The vaccine efficacies were evaluated by intraperitoneal injection in juvenile tilapia with each vaccine containing 1.8 × 106 TCID50 inactivated virus, followed by a booster dose at 21-day post primary vaccination (dppv) in the same manner. At 28 dppv, the fish were challenged with a lethal dose of TiLV. Expression of five immune genes in head kidney and …
Prevalence Of Canine Atopic Dermatitis And Distribution Of Causative Allergens In Bangkok,
2021
Faculty of Veterinary Science
Prevalence Of Canine Atopic Dermatitis And Distribution Of Causative Allergens In Bangkok, Sopon Sornsanit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine atopic dermatitis (CAD) is a pruritic allergic skin disease caused by an overreaction to environmental allergens. The diagnosis of CAD is made on the basis of the patient's history, physical examination, and exclusion of other pruritic skin diseases. Allergen-specific IgE serology (ASIS) is one of the most frequently recommended tests in veterinary dermatology for identifying causative allergens. The purpose of this study was to determine the prevalence of CAD and the types of allergens that cause it in atopic dogs using ASIS in the Bangkok metropolitan area. The study was divided into two sections. Between 2015 and 2019, 383 …
Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species,
2021
South Dakota State University
Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam
Electronic Theses and Dissertations
BVDV is a major pathogen of ruminants that causes immune dysfunction and lead to secondary bacterial infection. The underlying mechanism of this dysfunction is not well understood. Based on previous results, we believe that macrophages play a central role in BVDV-associated immune dysfunction. Previously, we have shown that the infected monocyte-derived macrophage (MDM) supernatant of the high virulent strains of BVDV significantly induced lymphocyte apoptosis. In the current study, we performed experiments to identify the apoptotic factors in the infected MDM supernatants in vitro. Also, we have extended our investigation to include testing the impact of infected macrophages on neutrophil …
Genetic Variation In The Bovine Myogenic Determination Factor 1 (G.782g>Apolymorphism) And Its Influence On Carcass Traits In Turkish Grey Steppe Cattle, Sena Ardiçli, Özden Çobanoğlu
Turkish Journal of Veterinary & Animal Sciences
MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic …
Effect Of Neem (Azadirachta Indica) And Tulsi (Ocimum Sanctum) Leaves Powder Ongrowth Performance, Hemato-Biochemical Profile And Carcass Traits Of Japanese Quail (Coturnix Japonica), Luqman Arshad, Syed Zeeshan Haider Naqvi, Jibran Hussain, Hala Naeem, Saima Shahzad, Amna Aslam, Abd Ur Rehman, Abdul Ghayas
Turkish Journal of Veterinary & Animal Sciences
The current research investigated the growth performance, hematology, serum biochemistry of Japanese quails fed Neem (Azadirachta indica) and Tulsi (Ocimum sanctum) leaves powder with basic diet. A total number of 300 one-week old Japanese quail chicks were divided randomly into four dietary treatments with five replicates each (15 birds per replicate). Dietary treatments were: T0 or control or basal diet, T1 (Neem 0.5%), T2 (Tulsi 1%) and T3 (Neem 0.25% + Tulsi 0.5%) in basal diet. Growth performance parameters (feed intake, body weight, weight gain and feed conversion rate) were determined weekly. Hematology, serum biochemistry, carcass traits were evaluated on …
Elastography Acoustic Radiation Force Impulse (Arfi): An Investigation Of Ocularelasticity In Dogs With Chronic Secondary Glaucoma, Gabriela Morais Madruga, Marcus Antonio Rossi Feliciano, Marjury Cristina Maronezi, Marcella Rosa Filézio, Thaís Guimarães Morato Abreu, Alexandre Pinto Ribeiro, Feancimery Aparecida Fachini, Karina Kamachi Kobashigawa, Igor Cezar Kniphoff Da Cruz, Ricardo Andres Ramirez Uscategui, José Luiz Laus
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to evaluate and compare the elasticity of the optic nerve (ON) and retinal-choroid-sclera complex (RCSC) in dogs with healthy eyes and secondary glaucoma using ARFI elastography. ARFI elastography was performed on normal eyes (n = 25) and affected by glaucoma (n = 25). The mean values of shear wave velocity (SWV) of the ON and RCSC were compared between groups by the Student?s t-test. The correlation between elastography measurements of the ON and RCSC and the intraocular pressure (IOP) was measured. The SWV of the optic nerve in glaucomatous eyes (4.77 ± 1.59 m/s) …
Identification Of Micrornas In The Follicular And Luteal Tissues Of Holstein-Friesian Cows,
2021
TÜBİTAK
Identification Of Micrornas In The Follicular And Luteal Tissues Of Holstein-Friesian Cows, Selçuk Özdemi̇r, Seli̇m Çomakli
Turkish Journal of Veterinary & Animal Sciences
MicroRNAs (miRNAs) regulate many physiological pathways, including development, cell differentiation, immune response as well as diseases by post-transcriptional processes. However, information is limited regarding the biological roles of miRNAs in the development of the follicular and luteal tissues in cows. The main aim of the present study was to identify miRNAs that are expressed in the follicular and luteal tissues of cows. We used a comparative RNA sequencing method to identify miRNA candidates that might play key roles in the follicular and luteal phases of Holstein cows. Nine miRNAs were expressed at a high ratio: 8 in the follicular tissue …
Leukocyte Esterase Reagent Strips: Rapid, Economical And Reliable Cow Side Test Forsubclinical Endometritis,
2021
TÜBİTAK
Leukocyte Esterase Reagent Strips: Rapid, Economical And Reliable Cow Side Test Forsubclinical Endometritis, Arshaq Asfar, Khursheed Ahmad Sofi, Mudasir Ahmad Bhat, Abrar Ahad Malik, Asloob Ahmad Malik
Turkish Journal of Veterinary & Animal Sciences
This study was carried out in crossbred cows (>60 DIM) to evaluate leukocyte esterase (LE) strip test, protein strip test, pH strip test, white side test (WST) of estrual mucus, in comparison to endometrial cytology (EC) using cytobrush (CB) as reference diagnostic method for subclinical endometritis (SCE). Endometrial cytology was done in 78 cows with ≥4% PMNs as cut-off value for SCE. Leukocyte esterase strip test was done by contacting estrual mucus with multireagent urinalysis reagent strips and results recorded after 10 min. Similarly, protein and pH strip tests were conducted using the same strips and results recorded after …
The The Effect Of Ketones On Estrogen, Progesterone, Ovulatory Follicle Size, Estrus And Fertility In Holstein Cows,
2021
TÜBİTAK
The The Effect Of Ketones On Estrogen, Progesterone, Ovulatory Follicle Size, Estrus And Fertility In Holstein Cows, Faezeh Pishvaei, Maryam Karimi Dehkordi, Mohammad Reza Nazem
Turkish Journal of Veterinary & Animal Sciences
This study aimed to investigate the effect of hyperketonemia on estrogen, progesterone and ovarian follicle size during postpartum as well as on pregnancy rate at first insemination. Regarding this, 90 Holstein cows were selected from one of the industrial dairy farms in Isfahan province, Iran. Estrus synchronization was routinely applied to all cows using the Ovsynch method. The blood sample of the cows was taken two times. The first blood sample was taken in 7-14 days after calving; to measure betahydroxybutyrate (BHBA), and the second sample was taken at the first insemination to measure the 17-ß oestradiol and progesterone hormones. …
The Effect Of Rangeland Quality On The Mohair Quality Of Angora Goats Fed On The Natural Rangelands,
2021
TÜBİTAK
The Effect Of Rangeland Quality On The Mohair Quality Of Angora Goats Fed On The Natural Rangelands, Betül Zehra Sariçi̇çek
Turkish Journal of Veterinary & Animal Sciences
The aim of this study is to determine the effect of the rangeland quality of Ankara goats fed on some natural rangeland in Ankara on the mohair quality. The research was carried out in 3 different quality natural rangelands in Ankara province. Crude protein, ether extract, Ca, Fe, N, energy contents and digestibility, in vitro gas production, and relative feed values of good quality rangeland plants were the highest compared to other rangelands, but the crude fiber, acid detergent fiber, and neutral detergent fiber were the lowest (p < 0.05). In the study were used a total of 120 animals (20 males, 20 females in each farm) from 3 farms where Angora goats are raised in the region of rangelands. The mohair quality was determined in mohair samples taken from 6 month old, 1.5, and 2.5-yearold animals. Rangeland quality significantly affected mohair lengths, fineness, elasticity, tenacity, and clean mohair yield, in males and females (p < 0.05). The highest values were obtained from goats fed good quality rangeland (p < 0.05). The effect of age on these features was also important. The mohair quality (length, elasticity, tenacity) increased with increasing age (p < 0.05). The effect of the rangeland quality on the nozzle number was not significant, but the effect of age was significant. and, the nozzles number increased with increasing age. The nozzle depth was affected by the rangeland quality and age. The nozzle depth has increased due to the increase in rangeland quality and age. While the mohair Ca, Mg, S, Fe, and N content of male and female goats were higher in goats fed on good quality rangeland, these were lower in goats fed low-quality rangeland (p < 0.05). While mohair Na content positively affected rangeland quality in females (p < 0.01), was insignificant in males.
Details On The Histomorphological Characteristics Of Marine Sea Pen, Virgularia Gustaviana, From The Persian Gulf,
2021
TÜBİTAK
Details On The Histomorphological Characteristics Of Marine Sea Pen, Virgularia Gustaviana, From The Persian Gulf, Zeinab Rezaei, Abdolali Movahedinia, Negin Salamat, Mohammad Sharif Ranjbar
Turkish Journal of Veterinary & Animal Sciences
Sea pens or pennatulaceans (Phylum Cnidaria, Order Pennatulacea) are colonial marine octocorals that typically anchor themselves into soft sediment. They make large colonies from the intertidal zone down to the abyssal plain that provide an important structural habitat to other organisms. In the present study, the morphological and histological characteristics of the dominant species of Virgularia gustaviana, the dominant sea pen species of the Persian Gulf, were surveyed. Samples of V. gustaviana were collected along the coastal intertidal zone of the Soru and Bandar Abbas beaches, located at the northeast of the Persian Gulf. The sea pens were then fixed …
Technical Staffs' Knowledge And Attitudes Survey Regarding Tail Docking On Dairy Farms Of Turkey,
2021
TÜBİTAK
Technical Staffs' Knowledge And Attitudes Survey Regarding Tail Docking On Dairy Farms Of Turkey, Çağri Çağlar Si̇nmez, İsmai̇l Ülger, Gökhan Aslim, Ali̇ Yi̇ği̇t, Mahmut Kali̇ber
Turkish Journal of Veterinary & Animal Sciences
The study examined the knowledge, beliefs, and attitudinal drivers of technical staff and breeders regarding tail docking and the incidence of docking on large-scale dairy farms (≥100 cattle) in Turkey. Survey responses were analyzed using the Mann?Whitney U test to determine attitudinal differences between respondents who self-reported docking tails or not docking their cattle. Using face-toface interviews of 210 respondents at 206 Turkish dairy farms was carried out. Tail docking procedures were practiced on 27.1% of the surveyed farms. At the time of the tail docking, 57.9% of cattle were less than 12 months of age. The most frequent tail …
Identification Of Novel Genetic Variants For Kap1.1, Kap1.3 And K33 Genes In Some Of Indigenous Goat Breeds Of Turkey,
2021
TÜBİTAK
Identification Of Novel Genetic Variants For Kap1.1, Kap1.3 And K33 Genes In Some Of Indigenous Goat Breeds Of Turkey, Razi̇ye Işik, Ayla Fi̇dan, Mehmet İhsan Soysal, Emel Özkan Ünal
Turkish Journal of Veterinary & Animal Sciences
The animal fibres such as mohair, cashmere and cashgora have a complex structure and affected by genetic variation of keratin associated protein genes as KAP 1.1 (Keratin Associated Protein 1.1, formerly known as B2A), KAP1.3 (Keratin Associated Protein 1.3, formerly known as B2C) and K33 (Keratin Intermediate Filaments Type I, formerly known as KRT1.2). Keratin-associated proteins play a significant role in identifying structural and mechanical properties of the hair and wool fibres. This study was conducted to detect genetic variation at the KAP1.1, KAP1.3 and K33 genes in indigenous Turkish goat populations using DNA sequencing method. The DNA of 100 …
Investigation Of The Nontypical Pasteurella Multocida Strains Obtained From Multiple Sources, Regions, And Times: An Unexpected Increase Was Detected, Asli Sakmanoğlu, Ali̇ Uslu, Zafer Sayin, Yasemi̇n Karyeyen, Gökçenur Sani̇oğlu Gölen, Ayşegül İlban, Beatri̇z Padron-Perez, Mustafa Agah Teki̇ndal, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
In this study, it was aimed to isolate Pasteurella multocida from 1250 lung samples (calf, sheep, and goat), with respiratory system infection, such as nasal discharge, cough, fever, and to investigate the phenotypic and genotypic properties of this bacteria. As a result, a total of 92 P. multocida were obtained, of which 66 (71.73%) were from calf, 19 (20.65%) from sheep, and 7 (7.6%) from goat. The A:3A type was the most common type with 34 isolates (36.95%), including 30 from calves (45.45%), and 4 from sheep (21.05%). An alternate mPCR protocol was developed to determine capB and capE genes …
A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods,
2021
TÜBİTAK
A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods, Sami̇ Gökpinar, Aycan Nuri̇ye Gazyağci, Meral Aydeni̇zöz, Ufuk Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kırıkkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with …
Seroprevalence Of Bovine Viral Diarrhoea Virus, Bovine Herpesvirus Type 1 And Bovineherpesvirus Type 4 Infections In Cattle In Ağrı Province, Eastern Anatolia Region, Turkey, Mustafa Si̇nan Aktaş, Hasan Çeli̇k
Turkish Journal of Veterinary & Animal Sciences
This research was carried out to determine the seroprevalence of Bovine Viral Diarrhoea Virus (BVDV), Bovine Herpes Virus 1 (BoHV-1), and Bovine Herpes Virus 4 (BoHV-4) in cattle (n = 188) in Ağrı province of Eastern Anatolia Region, Turkey. The presence of BVDV, BoHV-1, and BoHV-4 antibodies in sera samples were determined by an ELISA test kit. It was determined that the highest seropositivity for BoHV-1 in Tutak disrict (9.9%), in ≤ 3 years age groups (3.39%), and in Simmental cattle (14.28%); for BoHV-4 in Doğubeyazıt disrict (4.16%), %), in ≤ 3 years age groups (1.70%), and in Simmental cross …
