Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model,
2021
Faculty of Veterinary Science
Efficacies Of Thai Probiotic Strains Against Antibiotic Resistance Bacteria And Modulate Gut Microbiome And Resistome In Pig Model, Prasert Apiwatsiri
Chulalongkorn University Theses and Dissertations (Chula ETD)
Lactic acid bacteria (LAB) have been widely used as probiotics in the livestock industry because of their high potential for antibacterial activity, anticonjugation, antibiofilm capacity, and gut microbiome regulation. Lactobacillus plantarum 22F, 25F (L22F and L25F), and Pediococcus acidilactici 72N (P72N) showed several promising in vitro and in vivo properties, according to our recent research. The objective of this study was to evaluate the Thai LAB efficacy on anticonjugation and antibiofilm activities in E. coli harboring the mcr-1 gene. When compared to the neutralizing cell-free supernatant (CFS), we discovered that the CFS derived from our LAB strains at 1:16 dilution …
In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells,
2021
Faculty of Veterinary Science
In Vitro Generation Of Transplantable Insulin-Producing Cells From Canine Adipose-Derived Mesenchymal Stem Cells, Quynh Le Dang
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine mesenchymal stem cells (cMSCs) have potential applications for regenerative therapy, including the generation of insulin-producing cells (IPCs) for studying and treating diabetes. In this study, we established a useful protocol for generating IPCs from canine adipose mesenchymal stem cells (cAD-MSCs). Subsequently, in vitro preservation of pluronic F127-coated alginate (ALGPA)-encapsulated cAD-MSC-derived IPCs was performed to verify ready-to-use (RTU) IPCs. IPCs were induced from cAD-MSCs with the modulated three-stepwise protocol. The first step of definitive endoderm (DE) induction showed that the cooperation of Chir99021 and Activin A created the effective production of Sox17-expressed DE cells. The second step for pancreatic endocrine …
Active And Passive Immune Responses And Protection Of Nile Tilapia (Oreochromis Niloticus) Immunized With Tilapia Lake Virus Inactivated Vaccines,
2021
Faculty of Veterinary Science
Active And Passive Immune Responses And Protection Of Nile Tilapia (Oreochromis Niloticus) Immunized With Tilapia Lake Virus Inactivated Vaccines, Thao Thu Mai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Tilapia tilapinevirus, also known as tilapia lake virus (TiLV), is a contagious viral pathogen, resulting in mass mortalities and economic losses for tilapia industry. Here, we developed two simple cell-culture, heat-killed and formalin-killed vaccines (HKV and FKV) aiming to prevent this disease. The vaccine efficacies were evaluated by intraperitoneal injection in juvenile tilapia with each vaccine containing 1.8 × 106 TCID50 inactivated virus, followed by a booster dose at 21-day post primary vaccination (dppv) in the same manner. At 28 dppv, the fish were challenged with a lethal dose of TiLV. Expression of five immune genes in head kidney and …
Prevalence Of Canine Atopic Dermatitis And Distribution Of Causative Allergens In Bangkok,
2021
Faculty of Veterinary Science
Prevalence Of Canine Atopic Dermatitis And Distribution Of Causative Allergens In Bangkok, Sopon Sornsanit
Chulalongkorn University Theses and Dissertations (Chula ETD)
Canine atopic dermatitis (CAD) is a pruritic allergic skin disease caused by an overreaction to environmental allergens. The diagnosis of CAD is made on the basis of the patient's history, physical examination, and exclusion of other pruritic skin diseases. Allergen-specific IgE serology (ASIS) is one of the most frequently recommended tests in veterinary dermatology for identifying causative allergens. The purpose of this study was to determine the prevalence of CAD and the types of allergens that cause it in atopic dogs using ASIS in the Bangkok metropolitan area. The study was divided into two sections. Between 2015 and 2019, 383 …
Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species,
2021
South Dakota State University
Viral And Immune Studies On Bovine Viral Diarrhea Virus (Bvdv) In Bovine Species, Karim Abdelsalam
Electronic Theses and Dissertations
BVDV is a major pathogen of ruminants that causes immune dysfunction and lead to secondary bacterial infection. The underlying mechanism of this dysfunction is not well understood. Based on previous results, we believe that macrophages play a central role in BVDV-associated immune dysfunction. Previously, we have shown that the infected monocyte-derived macrophage (MDM) supernatant of the high virulent strains of BVDV significantly induced lymphocyte apoptosis. In the current study, we performed experiments to identify the apoptotic factors in the infected MDM supernatants in vitro. Also, we have extended our investigation to include testing the impact of infected macrophages on neutrophil …
Genetic Variation In The Bovine Myogenic Determination Factor 1 (G.782g>Apolymorphism) And Its Influence On Carcass Traits In Turkish Grey Steppe Cattle, Sena Ardiçli, Özden Çobanoğlu
Turkish Journal of Veterinary & Animal Sciences
MyoD1 gene is a member of the myogenic differentiation gene family, which plays a crucial role in growth and development. There is a lack of information about the MyoD1variants and their phenotypic influences in Turkish Grey Steppe cattle. Therefore the present study aimed to determine the genetic variability of MyoD1 g.782G>A polymorphism and to evaluate its effects on carcass characteristics in Turkish Grey Steppe cattle. Analyses were conducted on a total of 142 Turkish Grey Steppe bulls. The PCR-RFLP technique was used for genotyping of the g.782G>A polymorphism in exon 1 of the MyoD1 gene. The population genetic …
Effect Of Neem (Azadirachta Indica) And Tulsi (Ocimum Sanctum) Leaves Powder Ongrowth Performance, Hemato-Biochemical Profile And Carcass Traits Of Japanese Quail (Coturnix Japonica), Luqman Arshad, Syed Zeeshan Haider Naqvi, Jibran Hussain, Hala Naeem, Saima Shahzad, Amna Aslam, Abd Ur Rehman, Abdul Ghayas
Turkish Journal of Veterinary & Animal Sciences
The current research investigated the growth performance, hematology, serum biochemistry of Japanese quails fed Neem (Azadirachta indica) and Tulsi (Ocimum sanctum) leaves powder with basic diet. A total number of 300 one-week old Japanese quail chicks were divided randomly into four dietary treatments with five replicates each (15 birds per replicate). Dietary treatments were: T0 or control or basal diet, T1 (Neem 0.5%), T2 (Tulsi 1%) and T3 (Neem 0.25% + Tulsi 0.5%) in basal diet. Growth performance parameters (feed intake, body weight, weight gain and feed conversion rate) were determined weekly. Hematology, serum biochemistry, carcass traits were evaluated on …
Leukocyte Esterase Reagent Strips: Rapid, Economical And Reliable Cow Side Test Forsubclinical Endometritis,
2021
TÜBİTAK
Leukocyte Esterase Reagent Strips: Rapid, Economical And Reliable Cow Side Test Forsubclinical Endometritis, Arshaq Asfar, Khursheed Ahmad Sofi, Mudasir Ahmad Bhat, Abrar Ahad Malik, Asloob Ahmad Malik
Turkish Journal of Veterinary & Animal Sciences
This study was carried out in crossbred cows (>60 DIM) to evaluate leukocyte esterase (LE) strip test, protein strip test, pH strip test, white side test (WST) of estrual mucus, in comparison to endometrial cytology (EC) using cytobrush (CB) as reference diagnostic method for subclinical endometritis (SCE). Endometrial cytology was done in 78 cows with ≥4% PMNs as cut-off value for SCE. Leukocyte esterase strip test was done by contacting estrual mucus with multireagent urinalysis reagent strips and results recorded after 10 min. Similarly, protein and pH strip tests were conducted using the same strips and results recorded after …
A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods,
2021
TÜBİTAK
A Research On Babesia And Theileria Species In Sheep And Goats Of Kırıkkale Provincethrough Molecular Methods, Sami̇ Gökpinar, Aycan Nuri̇ye Gazyağci, Meral Aydeni̇zöz, Ufuk Kaya
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kırıkkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with …
Zilpaterol And Ractopamine: Reaching Consensus On Trace-Level Transfers To Racehorses,
2021
University of Kentucky
Zilpaterol And Ractopamine: Reaching Consensus On Trace-Level Transfers To Racehorses, Jacob Machin, Kimberly Brewer, Clara K. Fenger, Sarah White-Springer, Abelardo Morales-Briceno, Thomas Tobin
Maxwell H. Gluck Equine Research Center Faculty Publications
In this article, we review the investigation of trace amounts of b-2 adrenergic agonists, a drug class that possess significant risk to the integrity of horse racing whether intentionally used or through the inadvertent contamination of feed.
Predicting Tomorrow: Optimizing The Early Detection Of Disease And Disease Recovery In Dairy Calves Using Precision Technologies,
2021
University of Kentucky
Predicting Tomorrow: Optimizing The Early Detection Of Disease And Disease Recovery In Dairy Calves Using Precision Technologies, Melissa Cantor
Theses and Dissertations--Animal and Food Sciences
The leading causes of morbidity and mortality in preweaned dairy calves are diarrhea and bovine respiratory disease complex (BRD). The delayed detection of these diseases in calves can also delay intervention and disease recovery. The overarching objective of this dissertation was to follow a cohort of calves daily for the first 90 days of life for naturally occurring clinical BRD bouts and diarrheal bouts. The objective answered if feeding behaviors and activity levels were different in at-risk calves during BRD development and BRD recovery from an antimicrobial intervention. Furthermore, the potential of colostrum replacer as a feeding intervention strategy to …
Chemical Castration Using An Intratesticular Injection Of Mannitol: A Preliminary Studyin A Rat Model,
2021
TÜBİTAK
Chemical Castration Using An Intratesticular Injection Of Mannitol: A Preliminary Studyin A Rat Model, Mohammad-Ali Maadi, Mehdi Behfar, Amir Rasaei, Ali Shalizar-Jalali, Gholamreza Najafi, Vahid Mohammadi
Turkish Journal of Veterinary & Animal Sciences
This study was aimed to evaluated chemical sterilization using an intra-testicular injection of mannitol 20% in rats. Fifty male Wistar rats were divided into five groups, including 1) intact, 2) surgical castration and, 3 to 5) intra-testicular injections of normal saline, hypertonic saline, and mannitol, respectively. The baseline testosterone levels were measured. Surgical castration was performed in group 2. Intra-testicular injections were performed in groups 3, 4, and 5. On day 60, the testosterone level was evaluated. Then, sperm count, viability, motility, morphological abnormalities and DNA damage were studied. Gonadosomatic index was recorded on day 60. Serum testosterone level was …
Comparison Of Feed Value Of Amaranthus Powellii Willd. Forage To Some Roughagefeeds,
2021
TÜBİTAK
Comparison Of Feed Value Of Amaranthus Powellii Willd. Forage To Some Roughagefeeds, Gökhan Fi̇li̇k, Ayşe Gül Fi̇li̇k
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to compare the chemical composition, in vitro digestibility, protein and energy value, roughage value of Amaranthus powellii Willd. forage to the most commonly used alfalfa hay and wheat straw in ruminant nutrition. Amaranthus powellii Willd. forage has the potential to be a third quality roughage according to its relative feed value assessment of 104.55 ± 0.67. In addition, the relative forage quality assessment developed for feeding dairy cattle was 97.73 ± 0.05 for Amaranthus powellii Willd. forage, which had a higher the relative forage quality value than alfalfa hay and wheat straw, and it …
Text Mining To Identify The Origin Of Chronic Wasting Disease,
2021
San Jose State University
Text Mining To Identify The Origin Of Chronic Wasting Disease, G. Webb
Faculty Research, Scholarly, and Creative Activity
Chronic wasting disease (CWD) is a 100 percent fatal, prion disease of deer that has the potential to decimate the deer population and jump to the human population. A simple Google search on the “origin of chronic wasting disease” yielded a total of 56 relevant articles. Of these, 75 percent report that the origin is unknown, 19.6 percent report that the disease may have originated in a Fort Collins, Colorado, government research facility, and 5.4 percent report other possible origins. Government sources reported the Fort Collins theory 4.3 percent of the time while non-governmental sources, such as news articles, reported …
Female And Male-Controlled Livestock Holdings Impact Pastoralist Food Security And Women’S Dietary Diversity,
2021
University of Nebraska-Lincoln
Female And Male-Controlled Livestock Holdings Impact Pastoralist Food Security And Women’S Dietary Diversity, Henriette Gitungwa, Christopher Gustafson, E.Y. Jimenez, E. Wesley F. Peterson, M. Mwanzalila, Asha Makweta, E. Komba, R.R. Kazwala, J.A.K. Mazet, Elizabeth Vanwormer
School of Veterinary and Biomedical Sciences: Faculty Publications
Background: Food insecurity is a global problem that requires a One Health approach. As many households in low- and middle-income nations rely on crops and livestock that they produce to meet their household’s needs, food security and nutrition are closely linked to the health of animals and the environment. Resources controlled by women are more often allocated to uses that benefit the entire household, such as food, health, and educating children, than men’s resources. However, studies of gender control of resources among pastoralist societies are scant. We examined the effect of female and male control of livestock resources on food …
Heeding The Call Of Covid-19,
2021
Mayo Clinic
Heeding The Call Of Covid-19, David Wiebers, Valery Feigin
Animal Sentience
We are grateful to all of our commentators. They have provided a wide range of valuable perspectives and insights from many fields, revealing a broad interest in the subject matter. Nearly all the commentaries have helped to affirm, refine, expand, amplify, deepen, interpret, elaborate, or apply the messages in the target article. Some have offered critiques and suggestions that help us address certain issues in greater detail, including several points concerning industrialized farming and the wildlife trade. Overall, there is great awareness and strong consensus among commentators that any solution for preventing future pandemics and other related health crises must …
The Platelet Rich Plasma Injection Application For Canine Legg-Calvé-Perthes Disease Treatment,
2021
Faculty of Veterinary Science
The Platelet Rich Plasma Injection Application For Canine Legg-Calvé-Perthes Disease Treatment, Phanupong Chinnabrut
Chulalongkorn University Theses and Dissertations (Chula ETD)
The etiology of canine Legg-Calve-Perthes disease (LCPD) is still unknown. The current treatments are conservative and surgery. Although non-steroidal anti-inflammatory drugs (NSAIDs), nutraceuticals, and rehabilitation were used in the conservative treatment, it only had a 25% success rate. In a dog with severe clinical signs and bony change in radiography, the femoral head and neck ostectomy were selected. This procedure could decrease pain, but in clinical evaluation with force plate and kinetic and kinematic measurements, the dogs were shown to have lameness. The total hip replacement was done in a dog with good clinical results, but cost was a limitation …
Molecular Detection And Genetic Diversity Of Rotavirus A In Domestic Dogs And Cats In Bangkok, Thailand,
2021
Faculty of Veterinary Science
Molecular Detection And Genetic Diversity Of Rotavirus A In Domestic Dogs And Cats In Bangkok, Thailand, Ekkapat Chamsai
Chulalongkorn University Theses and Dissertations (Chula ETD)
Rotavirus A (RVA) in one of the important pathogens causing gastroenteritis in humans and many animal species. The zoonotic potential of RVA has been reported and raises major concerns, especially in high animal-human interfaces settings. The objectives of this thesis were to determine the occurrence of RVAs in dogs and cats, and to investigate the genetic diversity and genetic relationship among RVAs in dogs, cats, human, and other animal species. The total of 572 rectal swab samples were collected from dogs and cats in animal hospitals, in Bangkok and vicinities, during January 2020 to June 2021. The conventional one-step RT-PCR …
Selection Of Candidate Probiotic Bacteria From Chickens With Antagonism Against Campylobacter,
2021
Faculty of Veterinary Science
Selection Of Candidate Probiotic Bacteria From Chickens With Antagonism Against Campylobacter, Sirawich Danwanichwong
Chulalongkorn University Theses and Dissertations (Chula ETD)
Campylobacter is one of the most common causes of bacterial foodborne disease in humans worldwide. The effect of probiotics on Campylobacter control in poultry is inconsistent or rarely observed. For this reason, research on probiotics for Campylobacter control in poultry is required. Therefore, the objective of this study was to identify lactobacilli, Bacillus or Enterococcus strains isolated from Campylobacter-negative chickens which have the ability to inhibit the growth of Campylobacter in vitro and demonstrate favorable probiotic characteristics. A total of 602 bacterial isolates from Campylobacter-negative broiler chickens were assessed for their probiotic properties including Campylobacter inhibiting activity test, hemolytic activity …
Stress, Burnout, And Well-Being In New Veterinary Graduates: Evaluating A Pilot Online Professional Development Program,
2021
University of Kentucky
Stress, Burnout, And Well-Being In New Veterinary Graduates: Evaluating A Pilot Online Professional Development Program, Addie Rose Reinhard
Theses and Dissertations--Community & Leadership Development
Suicide, stress, and burnout are occurring at high rates among veterinary professionals, and the transition from student to practicing veterinarian has been shown to be a particularly stressful time. The aim of this study was to evaluate an online professional development program for new veterinary graduates incorporating peer social support and training in professional skills important for success in the transition to practice. In this mixed methods study, the program was evaluated qualitatively with focus group data and quantitatively by assessing knowledge gained and levels of stress, burnout, social support, and well-being. Seven new veterinary graduates participated in the program, …
