Virulence And Drug Resistance Of Aeromonas Veronii Isolated From Shellfish,
2021
Chulalongkorn University
Virulence And Drug Resistance Of Aeromonas Veronii Isolated From Shellfish, Yu-Xuan Xu, Hai-Peng Zhang, Guan-Yi Xu, Inam Muhamamda, Wen-Long Dong, Yi-Ming Wang, Ling-Cong Kong, Hong-Xia Ma
The Thai Journal of Veterinary Medicine
Aeromonas veronii (A. veronii) is one of the important zoonotic pathogens. The development of drug resistance and enrichment of resistant genes not only cause misery from bacterial diseases but also pose a threat to public health security. In this study, A. veronii was isolated and identified from 357 shellfish samples imported from 6 aquaculture bases in Jilin Province, China, and tested for virulence, tolerance and drug resistance genes. According to 16S rRNA and gyrB gene PCR result, 47 strains were identified as Aeromonas veronii. The distribution of 9 virulence factors including fla (55.3%), Alt (42.5%), Act (31.9%), Aer (31.9%), Lip …
Early Pregnancy Diagnosis In Water Buffaloes Through Detection Of Pregnancy-Associated Glycoprotein (Pag) In Milk Using Enzyme-Link Immunosorbent Assay,
2021
Chulalongkorn University
Early Pregnancy Diagnosis In Water Buffaloes Through Detection Of Pregnancy-Associated Glycoprotein (Pag) In Milk Using Enzyme-Link Immunosorbent Assay, Roseline D. Tadeo, Eufrocina P. Atabay, Edwin C. Atabay, Danica D. Matias, Zeshalyn P. Fajardo, Jhon Paul R. Apolinario, Carlito F. Dela Cruz, Ramesh C. Tilwani
The Thai Journal of Veterinary Medicine
Early pregnancy diagnosis following insemination is an important component of the reproductive management program to shorten the calving interval in water buffaloes. A recent method to detect pregnancy in dairy animals is through pregnancy-associated glycoproteins (PAGs) that are present during implantation and throughout the gestation period. In this study, milk PAG Enzyme-Link Immunosorbent Assay (ELISA) technique was used and run in parallel with blood PAG ELISA for its efficiency in detecting early pregnancy in lactating buffaloes. Fixed Time Artificial Insemination (FTAI) was conducted in lactating buffaloes followed by the collection of milk and blood samples postFTAI. Samples were then assayed …
Prevalence Of Canine Vector-Borne Blood Parasites In The Plain Regions Of Cambodia,
2021
Chulalongkorn University
Prevalence Of Canine Vector-Borne Blood Parasites In The Plain Regions Of Cambodia, Koemseang Nhuong, Samut Sum, Piyanan Taweethavonsawat
The Thai Journal of Veterinary Medicine
Canine vector-borne blood parasites (CVBP) cause various diseases in dogs. The aim of this study was to survey canine blood parasites in the plain regions of Cambodia. The study was conducted from November 2014 to September 2015. A total of 455 blood samples were collected from rural and urban areas in the plain regions of Cambodia. Six infected dogs were found (1.32%), with the following parasites: Dirofilaria immitis (2/6), Brugia pahangi (1/6), Babesia vogeli (1/6) and two co-infections of Ehrlichia canis with Anaplasma platys (1/6) and Babesia vogeli with Anaplasma platys (1/6). However, the rate of detection of blood parasitic …
Reverse Transcription Loop-Mediated Isothermal Amplification Combined With Lateral Flow Device (Rt-Lamp-Lfd) For Swine Influenza Virus Detection,
2021
Chulalongkorn University
Reverse Transcription Loop-Mediated Isothermal Amplification Combined With Lateral Flow Device (Rt-Lamp-Lfd) For Swine Influenza Virus Detection, Albarriyati Ummi Asih, Taveesak Janetanakit, Chanakarn Nasamran, Napawan Bunpapong, Supanat Boonyapisitsopa, Alongkorn Amonsin
The Thai Journal of Veterinary Medicine
Swine influenza virus (SIV) causes respiratory disease in pigs leading to economic losses in swine production. Some SIV subtypes can also infect human and considered as a zoonotic pathogen. The importants of swine influenza are not only impact on the economic losses but also the human health. The objectives of this study were to develop a reverse transcription loop-mediated isothermal amplification combined with lateral flow device assay (RT-LAMP-LFD) for the detection of SIVs and to evaluate the performance, sensitivity and specificity of the assay. Our result showed that RTLAMP-LFD assay was developed with six newly designed primers and with optimum …
Survey Of Knowledge And Opinion Among Thai Veterinarians On Nicotine Toxicity And Second-Hand Smoke Effects On Pets’ Health+,
2021
Chulalongkorn University
Survey Of Knowledge And Opinion Among Thai Veterinarians On Nicotine Toxicity And Second-Hand Smoke Effects On Pets’ Health+, Pongsri Srimoragot, Pratuang Sudsakorn, Phornchai Suwannaphirom, Kanin Ruchisereekul
The Thai Journal of Veterinary Medicine
There may be lack of knowledge in nicotine toxicity and second-hand smoke (SHS) effects, as well as the opinion of pets’ care among Thai veterinarians (vets). To examine knowledge, opinion of nicotine toxicity and SHS effects on dogs and cats’ health in Thai vets. A cross-sectional survey was applied. Data were collected using the self-reported questionnaire. Cronbach's alpha for the knowledge and opinion questionnaire was 0.90 and 0.94, respectively. After MUCIRB approval, systematic sampling was applied to 371 Thai vets. Data were analyzed using descriptive statistics. Thai vets were 5.7 percents current smokers. The majority of them had moderate knowledge …
Retrospective Evaluation Of The Patterns Of Clinical Use Of Anti-Parasitic Agents On Animals Presented For Veterinary Care At An Animal Hospital In Nigeria,
2021
Chulalongkorn University
Retrospective Evaluation Of The Patterns Of Clinical Use Of Anti-Parasitic Agents On Animals Presented For Veterinary Care At An Animal Hospital In Nigeria, Thelma Ebele Ihedioha, Isaac Uzoma Asuzu, John Anaelom Nwanta
The Thai Journal of Veterinary Medicine
Patterns of use of anti-parasitic agents (APAs) on animals presented for veterinary care at the Veterinary Teaching Hospital, Nsukka, Nigeria, from January 2013 to December 2017 were surveyed. Animals presented were predominantly dogs (84.0%) and goats (8.1%). A total of 4851 cases were evaluated, out of which 3196 (65.9%) were treated with APAs. There was a significant variation (p=0.002) in the annual frequency of use of APAs across the study period, but the effect size of the variation was small (ɸc=0.06). Ivermectin was the most frequently used APA (1479 out of the 3196 = 46.3%); followed by praziquantel-pyrantel-fenbendazole combined formulation …
Clinical Evaluation Of The Accuracy Of Foetal Radiographic Findings In Parturition Date Prediction Of Small Size Dogs,
2021
Chulalongkorn University
Clinical Evaluation Of The Accuracy Of Foetal Radiographic Findings In Parturition Date Prediction Of Small Size Dogs, Panthipa Borikappakul, Nardtiwa Chaivoravitsakul, Rampaipat Penchome, Suppawiwat Ponglowhapan
The Thai Journal of Veterinary Medicine
Ultrasonographic measurements of extra-foetal or foetal structures have been reported as an accurate means of predicting the date of parturition in the bitch. However, for many years, radiography has been clinically useful for pregnancy diagnosis, determining the number of foetuses and prolonged foetal death. Guidelines for estimating the canine parturition date based on foetal skeletal mineralization in relation to gestational age and preovulatory LH surge have been applied in small animal practice. The aims of this retrospective study were to evaluate the accuracy of parturition date prediction using foetal radiographic findings in small size dogs (1-10 kg), and to determine …
Evaluation Of Dermal Toxicity Study Of Ethanolic Extract Of Morinda Citrifolia Fruit In Spraque Dawley Rats,
2021
Chulalongkorn University
Evaluation Of Dermal Toxicity Study Of Ethanolic Extract Of Morinda Citrifolia Fruit In Spraque Dawley Rats, Farhan Hanif Reduan Mohd, Rosly Shaari Mohd, Nurul Syahirah Ahmad Sayuti, Abdullahi Aliyu, Noordin Mohamed Mustapha, Md Zuki Abu Bakar, Shanmugavelu Sithambaram, Hazilawati Hamzah
The Thai Journal of Veterinary Medicine
Plant-based preparations are widely known to complement dermatological therapy, however toxic profiles of these plants are lacking and limited. To investigate the toxicity of Morinda citrifolia fruit ethanol extract applied to the skin of rats daily for 28 days, thirty female Sprague Dawley rats were grouped into five groups (n=5 rats for each group) for each study. The experimental period was set at 14 days and 28 days for acute and subacute dermal toxicity, respectively. Rats in the treatment groups were topically applied with the plant extract at three different doses; 2.5%, 5% and 10% on a shaved area of …
Production Performance, Carcass Traits And Meat Quality Of Growing Naemi Lambs Fed A Diet Containing Palm Kernel Meal,
2021
Chulalongkorn University
Production Performance, Carcass Traits And Meat Quality Of Growing Naemi Lambs Fed A Diet Containing Palm Kernel Meal, Gamaledin M. Suliman, Hanee H. Al-Baadani, Mutassim M. Abdelrahman, Ibrahim A. Alhidary
The Thai Journal of Veterinary Medicine
Palm kernel meal (PKM) is a byproduct produced after oil extraction from palm kernels. It is a valuable source of fiber, energy and protein which can reduce the cost of total mixed ration (TMR). This study was conducted to assess the growth performance, carcass characteristics and meat quality of lambs fed TMR pellet with PKM. A total of 32, three-month-old male lambs were randomly distributed into two diets (PTMR1= 0; PTMR2= 200 kg PKM/ton TMR). Results of the study showed that the production performance and the characteristics, composition and fat content of carcasses, as well as meat quality traits were …
Nasal Colonization Of Pigs And Farm Attendants By Staphylococcus Aureus And Methicillin-Resistant Staphylococcus Aureus (Mrsa) In Kebbi, Northwestern Nigeria,
2021
Chulalongkorn University
Nasal Colonization Of Pigs And Farm Attendants By Staphylococcus Aureus And Methicillin-Resistant Staphylococcus Aureus (Mrsa) In Kebbi, Northwestern Nigeria, Mohammed Sani Gaddafi, Yusuf Yakubu, Abdulkadir Usman Junaidu, Mohammad Bashir Bello, Bashiru Garba, Asinamai Athliamai Bitrus, Habiba Lawal
The Thai Journal of Veterinary Medicine
Methicillin Resistant Staphylococcus aureus (MRSA) is a leading cause of nosocomial, community and livestockassociated colonization and infection worldwide. This study aimed at investigating the nasal colonization of pigs and farm attendants by S. aureus and MRSA in Kebbi, North-Western Nigeria. A cross-sectional study was conducted in Kebbi Northwestern Nigeria using simple random sampling. A total of 212 nasal swabs were collected from two farms consisting of 100 samples each from pigs and 12 samples from farm attendants. A well-structured questionnaire was administered for risk factor analysis. Nasal swabs were examined using routine bacteriological culture and identification. Presumptive MRSA was confirmed …
Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam,
2021
Chulalongkorn University
Identification Of Genotype And Phenotype Of Antimicrobial Resistance Of Escherichia Coli Isolates From Pigs In Southern Vietnam, Do Tien Duy, Nguyen Luong Lam Anh, Nguyen Thi Kim Thoa, Phitsanuwattana K, Thongratsakul S, Carrique-Mas Jj, Le Thanh Hien, Nguyen Thi Thu Nam, Nguyen Tat Toan
The Thai Journal of Veterinary Medicine
Escherichia coli is a primary reservoir of antimicrobial resistance, known chiefly for the container of AMR-encoding genes (ARGs), and poses potential risks to human and animal health. This study investigated AMR phenotypes and ARGs in 90 E. coli isolates from different pig groups in 10 farms in southern Vietnam. The minimum inhibitory concentration (MIC) of 19 common antimicrobial agents was determined, and polymerase chain reaction (PCR) was used to investigate seven ARGs (blaTEM, aadA1, strA, dfrA12, sul3, cmlA and tetA). Cohen’s kappa statistic (κ) was applied to assess the concordance between phenotypic and genotypic profiles. A total of 81.1% of …
Evaluation Of Protection Efficiency Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus In Broiler Chickens: Comparative In Field And In Laboratory Studies,
2021
Chulalongkorn University
Evaluation Of Protection Efficiency Of Different Vaccination Programs Against Velogenic Newcastle Disease Virus In Broiler Chickens: Comparative In Field And In Laboratory Studies, Serageldeen Sultan, Marwa Hamed, Nabila Osman
The Thai Journal of Veterinary Medicine
This study was conducted to evaluate the protective efficiency of different vaccines and vaccination programs against Newcastle disease virus (NDV) in chickens, in the field and the laboratory. In the field, three commercial chicken farms (LA, LB, and QC) used different types of live vaccines and vaccination programs via drinking water (DW) were serologically estimated by hemagglutination inhibition (HI) assay to detect the level of circulating antibodies against NDV. The laboratory study was carried out to assess whether the types of vaccine and their route of administration influence the HI level and viral shedding. Chicken groups (G1-G5) except for the …
The Occurrence Of Gastrointestinal Parasitic Infections Of Goats In Ratchaburi, Thailand,
2021
Chulalongkorn University
The Occurrence Of Gastrointestinal Parasitic Infections Of Goats In Ratchaburi, Thailand, Witchuta Junsiri, Pulice Tapo, Runglawan Chawengkirttikul, Amaya Watthanadirek, Napassorn Poolsawat, Sutthida Minsakorn, Panat Anuracpreeda
The Thai Journal of Veterinary Medicine
Gastrointestinal (GI) parasitic diseases play an important role in the major constraints on goat production in Thailand and worldwide. The aim of this study was to assess the occurrence of GI parasitic infections among goats in Ratchaburi province of Thailand. In this study, fecal samples (394) were collected and tested by means of the floatation, sedimentation and direct smear methods to discriminate eggs or oocyst of the parasites. The overall occurrence of GI parasitic infections was 86.54%. The respective occurrences for the raising distance, farm system, water source nearby, type of feed and aquatic plants were found to vary significantly …
Artificial Neural Networks Approach For Predicting Methionine Requirement In Broiler Chickens,
2021
Chulalongkorn University
Artificial Neural Networks Approach For Predicting Methionine Requirement In Broiler Chickens, Chanwit Kaewtapee, Charn Khetchaturat, Rattana Nukreaw, Nuttawut Krutthai, Chaiyapoom Bunchasak
The Thai Journal of Veterinary Medicine
The objective of this research was to apply artificial neural networks (ANNs) for predicting the methionine requirement in broiler chickens at day 1-10 (starter period) and day 11-21 (grower period). A total of 28 data was obtained from five hundred and sixty male broiler chicks (Ross 308), which were divided into twenty-eight pens with twenty chickens in each. Body weight was determined at days 10 and 21. A bootstrapping method was used to multiply the observations to overcome the limited data for training. A total of 280 data was obtained and divided into a training set (n = 220) and …
Infectious Endometritis In Arabian Mares: An Updated Clinical Investigation Of Uterine Microbial Isolates, Antimicrobial Sensitivities And Fertility In Egypt,
2021
Chulalongkorn University
Infectious Endometritis In Arabian Mares: An Updated Clinical Investigation Of Uterine Microbial Isolates, Antimicrobial Sensitivities And Fertility In Egypt, Amr S. El-Shalofy, Mohamed K. Derbala, Hanaa A. Asfour, Hany M. Eissa, Aly B. Aly
The Thai Journal of Veterinary Medicine
The present study aimed to survey the prevalence of microbial isolates in the uterus of sub-fertile Arabian mares and to identify their existing antimicrobial sensitivities. In addition, to evaluate the efficacy of antimicrobial therapy based on the fertility outcomes determined by sonographic pregnancy diagnosis. Double guarded uterine swabs were collected from (barren) Arabian mares during mid-estrus. Based on the laboratory findings of microbial culture and antimicrobial sensitivity, mares were treated, inseminated and tested for pregnancy two weeks after ovulation. The predominant microbial isolates were Beta-hemolytic streptococci (BHS) (38.9%), Escherichia coli (E. coli) (33.3%), Staphylococcus aureus (S. aureus) (20.4%) and Candida …
Molecular Evolutionary Analysis Of Orf3 And M Genes Of Porcine Epidemic Diarrhea Virus In Heilongjiang Province Of China,
2021
Chulalongkorn University
Molecular Evolutionary Analysis Of Orf3 And M Genes Of Porcine Epidemic Diarrhea Virus In Heilongjiang Province Of China, Yulong Zhou, Guoqiang Gao, Liang Zheng, Zhijun Wu, Hongwei Cao, Hua Zhang, Yanlong Cong
The Thai Journal of Veterinary Medicine
In this present study, 11 porcine epidemic diarrhea viruses (PEDVs) were isolated from the fecal samples of piglets infected with PEDV in HeiLongJiang province of China. In order to analyze the molecular evolution of these reemerging PEDVs, the ORF3 and M genes of 11 PEDVs were amplified by reverse transcriptase polymerase chain reaction, cloned, sequenced, and analyzed with each other as well as other PEDV reference strains. The complete nucleotide sequence comparison was performed with Bioedit software, and the results showed that the similarity of 11 ORF3 and M genes ranged from 97% to 100%. Nucleotides and amino acids were …
Successful Management Of Hypoadrenocorticism In A French Bulldog By A Smaller Dose And Lesser Frequency Of The Desoxycorticosterone Pivalate: A Case Report,
2021
Chulalongkorn University
Successful Management Of Hypoadrenocorticism In A French Bulldog By A Smaller Dose And Lesser Frequency Of The Desoxycorticosterone Pivalate: A Case Report, Phuttipan Channgam, Walasinee Sakcamduang
The Thai Journal of Veterinary Medicine
A 1 year and 4 months old, intact female French Bulldog was presented because of weakness, anorexia, and vomiting for 3 days. Laboratory findings included azotemia, hyponatremia, hypochloremia, sodium to potassium (Na:K) ratio was 23.88, and the lower level of basal cortisol concentration than the detection limit. The small size of both adrenal glands obtained from abdominal ultrasonography. Subsequently, the adrenocorticotropic hormone (ACTH) stimulation test was performed, and the diagnosis of hypoadrenocorticism was confirmed. The desoxycorticosterone pivalate (DOCP) with a smaller dose and lesser frequency than the recommendation of 2.2 mg/kg every 25 days. The dog received DOCP subcutaneous injection …
Factors Leading To Successful Island Rodent Eradications
Following Initial Failure,
2021
Manaaki Whenua- Landcare Research
Factors Leading To Successful Island Rodent Eradications Following Initial Failure, Araceli Samaniego, Peter Kappes, Keith Broome, Steve Cranwell, Richard Griffiths, Grant Harper, Pete Mcclelland, Russell Palmer, Gerard Rocamora, Keith Springer, David Will, Shane Siers
United States Department of Agriculture Wildlife Services: Staff Publications
Island rodent eradications are increasingly conducted to eliminate the negative impacts of invasive rodents. The success rate in the tropics has been lower than in temperate regions, triggering research and reviews. Environmental factors unique to the tropics (e.g., land crabs and year-round rodent breeding) have been associated with eradication failure. Operational factors have also been important, but these have not been comprehensively assessed. The environmental and operational factors using global cases where rodent eradication initially failed and subsequent attempts occurred were compared. It was determined whether operational factors explained the initial failures, whether operational improvements explained subsequent successes, and whether …
The Pelvic Flexure Separates Distinct Microbial Communities In The Equine Hindgut,
2021
Colorado State University
The Pelvic Flexure Separates Distinct Microbial Communities In The Equine Hindgut, Kailee J. Reed, Isabelle G. Z. Kunz, Jessica A. Scare, Martin K. Nielsen, Philip J. Turk, Robert J. Coleman, Stephen J. Coleman
Maxwell H. Gluck Equine Research Center Faculty Publications
As hindgut fermenters, horses are especially dependent on the microbiota residing in their cecum and large intestines. Interactions between these microbial populations and the horse are critical for maintaining gut homeostasis, which supports proper digestion. The current project was motivated to determine if any features of the fecal microbiota are informative of the microbial communities from the cecum, ventral colon, or dorsal colon. Digesta from the cecum, ventral colon, dorsal colon and feces were collected from 6 yearling miniature horses. Microbial DNA was isolated and the microbiota from each sample was characterized by profiling the V4 region of the 16S …
Genomic Comparisons Of Persian Kurdish, Persian Arabian And American Thoroughbred Horse Populations,
2021
University of Kentucky
Genomic Comparisons Of Persian Kurdish, Persian Arabian And American Thoroughbred Horse Populations, Navid Yousefi-Mashouf, Hassan Mehrabani-Yeganeh, Ardeshir Nejati-Javaremi, Ernest Bailey, Jessica L. Petersen
Maxwell H. Gluck Equine Research Center Faculty Publications
The present research aimed to characterize the Persian Kurdish horse population relative to the Persian Arabian and American Thoroughbred populations using genome-wide SNP data. Fifty-eight Kurdish, 38 Persian Arabian and 83 Thoroughbred horses were genotyped across 670,796 markers. After quality control and pruning to eliminate linkage disequilibrium between loci which resulted in 13,554 SNPs in 52 Kurdish, 24 Persian Arabian and 58 Thoroughbred horses, the Kurdish horses were generally distinguished from the Persian Arabian samples by Principal Component Analyses, cluster analyses and calculation of pairwise FST. Both Persian breeds were discriminated from the Thoroughbred. Pairwise FST between …
