Veterinary Medicine Commons^™

“Adopt-A-Tissue” Initiative Advances Efforts To Identify Tissue-Specific Histone Marks In The Mare, N. B. Kingsley, Natasha A. Hamilton, Gabriella Lindgren, Ludovic Orlando, Ernest Bailey, Samantha Brooks, Molly Mccue, Theodore S. Kalbfleisch, James N. Macleod, Jessica L. Petersen, Carrie J. Finno, Rebecca R. Bellone

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.

Long Non-Coding Rna Zfas1 Is A Major Regulator Of Epithelial-Mesenchymal Transition Through Mir-200/Zeb1/E-Cadherin, Vimentin Signaling In Colon Adenocarcinoma, Stephen J. O'Brien, Casey Fiechter, James Burton, Jacob Hallion, Mason Paas, Ankur Patel, Ajay Patel, Andre Rochet, Katharina Scheurlen, Sarah Gardner, Maurice Eichenberger, Harshini Sarojini, Sudhir Srivastava, Shesh Rai, Theodore S. Kalbfleisch, Hiram C. Polk, Susan Galandiuk

Veterinary Science Faculty Publications

Colon adenocarcinoma is a common cause of cancer-related deaths worldwide. Epithelial-mesenchymal transition is a major regulator of cancer metastasis, and increased understanding of this process is essential to improve patient outcomes. Long non-coding RNA (lncRNA) are important regulators of carcinogenesis. To identify lncRNAs associated with colon carcinogenesis, we performed an exploratory differential gene expression analysis comparing paired colon adenocarcinoma and normal colon epithelium using an RNA-sequencing data set. This analysis identified lncRNA ZFAS1 as significantly increased in colon cancer compared to normal colon epithelium. This finding was validated in an institutional cohort using laser capture microdissection. ZFAS1 was also found …

An Inactivated Bacterium (Paraprobiotic) Expressing Bacillus Thuringiensis Cry5b As A Therapeutic For Ascaris And Parascaris Spp. Infections In Large Animals, Joseph F. Urban, Martin K. Nielsen, David Gazzola, Yue Xie, Ethiopia Beshah, Yan Hu, Hanchen Li, Florentina Rus, Kelly Flanagan, Austin Draper, Sridhar Vakalapudi, Robert W. Li, Gary R. Ostroff, Raffi V. Aroian

Maxwell H. Gluck Equine Research Center Faculty Publications

Ascaris and Parascaris are important parasites in the family Ascarididae, large, ubiquitous intestinal-dwelling nematodes infecting all classes of vertebrates. Parasitic nematode drug resistance in veterinary medicine and drug recalcitrance in human medicine are increasing worldwide, with few if any new therapeutic classes on the horizon. Some of these parasites are zoonotic, e.g., Ascaris is passed from humans to pigs and vice versa. The development of new therapies against this family of parasites would have major implications for both human and livestock health. Here we tested the therapeutic ability of a paraprobiotic or dead probiotic that expresses the Bacillus …

Characterization Of Memory T-Lymphocyte Subsets In Dogs, Annie Beth Bauman

LSU Master's Theses

A more complete understanding of canine T-cell immunity is necessary for improving diagnostic and therapeutic approaches to canine diseases, developing cell-based canine immunotherapeutics, and evaluating dogs as large mammal models for comparative immunology research. Canine immunological studies are limited by the lack of established phenotypic markers identifying diverse differentiation states in canine T-cells. Differential expression of CD45RA (indicating antigen experience) and CD62L (indicating lymph node homing capability), shows promise as a tool to delineate canine naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector memory (TEMRA) T-cells. The aim of this study was to characterize canine memory T-cell …

Maldi-Tof Ms Biomarker Detection Models To Distinguish Rtx Toxin Phenotypes Of Moraxella Bovoculi Strains Are Enhanced Using Calcium Chloride Supplemented Agar, Matthew M. Hille, Michael L. Clawson, Aaron M. Dickey, Justin H. Lowery, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Moraxella bovoculi is the bacterium most often cultured from ocular lesions of cattle with infectious bovine keratoconjunctivitis, also known as bovine pinkeye. Some strains of M. bovoculi contain operons encoding for a repeats-in-toxin (RTX) toxin, which is a known virulence factor of multiple veterinary pathogens. We explored the utility of MALDI-TOF MS and biomarker detection models to classify the presence or absence of an RTX phenotype in M. bovoculi. Ninety strains that had undergone whole genome sequencing were classified by the presence or absence of complete RTX operons and confirmed with a visual assessment of hemolysis on blood agar. Strains …

Pioneer Science And The Great Plagues: How Microbes, War, And Public Health Shaped Animal Health, Norman F. Cheville, Purdue University Press

Purdue University Press Books

Pioneer Science and the Great Plagues covers the century when infectious plagues—anthrax, tuberculosis, tetanus, plague, smallpox, and polio—were conquered, and details the important role that veterinary scientists played. The narrative is driven by astonishing events that centered on animal disease: the influenza pandemic of 1872, discovery of the causes of anthrax and tuberculosis in the 1880s, conquest of Texas cattle fever and then yellow fever, German anthrax attacks on the United States during World War I, the tuberculin war of 1931, Japanese biological warfare in the 1940s, and today’s bioterror dangers.

Veterinary science in the rural Midwest arose from agriculture, …

Generation Of A Biobank From Two Adult Thoroughbred Stallions For The Functional Annotation Of Animal Genomes Initiative, Callum G. Donnelly, Rebecca R. Bellone, Erin N. Hales, Annee Nguyen, Scott A. Katzman, Ghislaine A. Dujovne, Kelly E. Knickelbein, Felipe Avila, Theodore S. Kalbfleisch, Elena Giulotto, Nicole B. Kingsley, Jocelyn Tanaka, Elizabeth Esdaile, Sichong Peng, Anna Dahlgren, Anna Fuller, Michael J. Mienaltowski, Terje Raudsepp, Verena K. Affolter, Jessica L. Petersen, Carrie J. Finno

Maxwell H. Gluck Equine Research Center Faculty Publications

Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior to humane euthanasia. Epididymal sperm was recovered from both stallions immediately postmortem and cryopreserved. Aseptically collected full thickness skin biopsies were used to isolate, culture and cryopreserve dermal fibroblasts. Serum, plasma, cerebrospinal fluid, urine, and gastrointestinal content from various locations were collected and cryopreserved. Under guidance of a board-certified veterinary anatomic …

The Effects Of Mechanical Rocking Motion On Transdifferentiating Feline Adipose Multipotent Stromal Cells Into Functioning Pancreatic Β-Cell Like Islets, Emily Halphen

Honors Capstones

No abstract provided.

Pharmacokinetics Of Oxfendazole Nanosuspension In Sheep, Yuzhu Sun, Xiaofang Wang, Aoxue Zhang, Bao Zhang, Liwei Shao, Yue Liu, Haiting Wang, Wanhe Luo, Dongmei Chen, Shuyu Xie

The Thai Journal of Veterinary Medicine

The pharmacokinetics of our previous prepared oxfendazole nanosuspension was studied in sheep to understand its enhancement of bioavailability. Ten sheep were randomly divided into two groups, each of which received a singledose (5mg/kg) oral oxfendazole nanosuspension and oxfendazole granules. After intragastric administration, oxfendazole rapidly reached peak concentrations of 11.25 μg/mL and 5.50 µg/mL at 5.6 h and 6.6 h in the nanosuspension group and granules group and the concentrations gradually reduced to below the detection limit at 96 h and 72h, respectively. The main pharmacokinetic parameters of oxfendazole after administration to the sheep: The Tmax, Cmax, AUClast, MRTlast, T1/2 and …

Investigation Of The Mechanical And Grounding Characteristics Of The Domestic Cat’S Paw Pads Based On Gait Analysis, Ye Mei, Guolin Wang, Haichao Zhou, Congzhen Liu

The Thai Journal of Veterinary Medicine

The paw pads, which can produce moderate changes that are reflected in mechanical and grounding characteristics, play an important role for domestic cats in realizing their biological characteristics. The purpose of this study was to investigate these moderate changes of paw pads in various gaits i.e. walking, trotting and emergency stop gaits based on the ground reaction forces and contact strain experiments to obtain the mechanical and grounding shape representations. The results proved that with the change of gait, the representations changed obviously. For the ground reaction forces, the response of the fore paw pads was more sensitive than that …

A Clinical Survey Investigating The Dental Disorders Of Jennies In China, Wuyan Jiang, Bo Liu, Yipeng Jin, Liang Deng, Qingchao Liao, Jing Li

The Thai Journal of Veterinary Medicine

Recent clinical studies have documented a high prevalence of dental disorders in donkeys. However, there are few studies about the same topic in China. A cross-sectional study was performed to investigate the prevalence of clinically diagnosed dental disorders of donkeys in China. A total of 101 jennies which had never received any dental care were examined for the presence of dental disorders under small-holder farm conditions. The prevalence of dental disorders was analyzed by Chi‐square test or Fisher's exact correlation test. The strength of association was evaluated using Cramer's V test. Jennies between 2 and 15 years of age were …

Brucellosis In A Water Buffalo (Bubalus Bubalis) Herd In Balikesir Province Of Turkey: A Bacteriological And Pathological Investigation, Hasan Özen, Ziya İlhan, Mustafa Usta, Musa Karaman, Fatma İlhan

The Thai Journal of Veterinary Medicine

Brucellosis, caused by a facultative intracellular bacterium, induces great economic loss in the livestock economy worldwide. In this study, the abortion problem in an Anatolian water buffalo herd was investigated by pathological, bacteriological and serological means. An aborted fetus as well as blood and milk samples from 4 buffalo cows with abortion history were studied. The thoracic and abdominal cavities of the aborted fetus were filled with serohemorrhagic fluid, and abomasum had yellowish brown fluid containing fibrous material in it. Bronchointerstitial pneumonia was recognized in the lung tissues. Brucella (B.) abortus biotype 3 was isolated from the aborted fetus. Blood …

Identification And Genotyping Of Chlamydia Abortus With Mlva From Ruminant Abortions In The Marmara Region Of Turkey, Mehmet Engin Malal, Suheyla Turkyilmaz

The Thai Journal of Veterinary Medicine

Abortion in ruminants is an important problem in Turkey and it leads to serious economic losses for farmers. The aim of this study is to diagnose Chlamydia abortus in ruminant abortions in the Marmara Region of Turkey and to determine the genotypes of the agent and obtain the first national epidemiological data in this sense. For this purpose, a total of 730 abortion materials (fetal tissue, fetal stomach contents, placentas, cotyledons, vaginal swabs) belonging to 267 cattle, 380 sheep, 70 goats and 13 water buffaloes were examined for C. abortus with species specific real-time PCR. DNAs of positive samples were …

Toxicity Of Sodium Nitrite-Based Vertebrate Pesticides For European Starlings (Sturnus Vulgaris), Scott J. Werner, Shelagh T. Deliberto, Hailey E. Mclean, Katherine E. Horak, Kirt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In the 21st century, invasive animals rank second only to habitat destruction as the greatest threat to global biodiversity. Socially-acceptable and cost-effective strategies are needed to reduce the negative economic and environmental impacts of invasive animals. We investigated the potential for sodium nitrite (SN; CAS 7632-00-0) to serve as an avian toxicant for European starlings (Sturnus vulgaris L.). We also assessed the non-target hazard of an experimental formulation of SN that is being developed as a toxicant for invasive wild pigs (Sus scrofa L.). In gavage experiments with European starlings, we identified a lowest observed adverse effect level …

Sars-Cov-2 Exposure In Escaped Mink, Utah, Usa, Susan A. Shriner, Jeremy W. Ellis, J. Jeffrey Root, Annette Roug, Scott R. Stopak, Gerald W. Wiscomb, Jared R. Zierenberg, Hon S. Ip, Mia Kim Torchetti, Thomas J. Deliberto

United States Department of Agriculture Wildlife Services: Staff Publications

In August 2020, outbreaks of coronavirus disease were confirmed on mink farms in Utah, USA. We surveyed mammals captured on and around farms for evidence of infection or exposure. Free-ranging mink, presumed domestic escapees, exhibited high antibody titers, suggesting a potential severe acute respiratory syndrome coronavirus 2 transmission pathway to native wildlife.

We report a wildlife epidemiologic investigation of mammals captured on or near properties in Utah, USA, where outbreaks of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection occurred in farmed mink. Mink farms are relatively common in the United States, and most are small family farms. The US …

Great Expectations: Deconstructing The Process Pathways Underlying Beaver-Related Restoration, Caroline S. Nash, Gordon E. Grant, Susan Charnley, Jason B. Dunham, Hannah Gosnell, Mark B. Hausner, David S. Pilliod, Jimmy Taylor

United States Department of Agriculture Wildlife Services: Staff Publications

Beaver-related restoration is a process-based strategy that seeks to address wide-ranging ecological objectives by reestablishing dam building in degraded stream systems. Although the beaver-related restoration has broad appeal, especially in water-limited systems, its effectiveness is not yet well documented. In this article, we present a process-expectation framework that links beaver-related restoration tactics to commonly expected outcomes by identifying the set of process pathways that must occur to achieve those expected outcomes. We explore the contingency implicit within this framework using social and biophysical data from project and research sites. This analysis reveals that outcomes are often predicated on complex process …

Avian Visual Ecologists Should Consider Uv Absorbance And All Sensory Modalities: A Response To Wisenden Et Al. (2020), Sean T. O'Daniels, Scott J. Werner, Ken Yasukawa

United States Department of Agriculture Wildlife Services: Staff Publications

In a recent publication, Wisenden et al. (2020) examined responses of territorial male Red-winged Blackbirds (Agelaius phoeniceus) to models constructed with ultraviolet (UV)-reflective red epaulets for the purpose of determining if the addition of UV reflectance to epaulets ("UV+") changed the effectiveness of signals to receivers relative to "control" epaulets under field conditions. The authors hypothesized that "UV+epaulet coloration represents a visual signal with increased efficacy in territorial interactions." They presented behavioral data but no visual modeling data. Our aims in this commentary are to suggest alternative terms to those used by the authors, to express concern about the use …

Detection Of Bacillus Cereus And Gram-Negative Bacteria Communities In Commercial Sesame In Japan, Kazuko Kato, Noriko Komagome, Machiko Mineki, Sumalee Boonmar, Yukio Morita

The Thai Journal of Veterinary Medicine

Sesame seeds are used in many traditional Japanese foods for their flavor and taste but commercial sesame can be highly contaminated with bacteria. We therefore examined the bacterial populations, including Bacillus spp., Enterobacteriaceae bacteria and standard plate count bacteria in sesame samples purchased from markets in Japan. A total of 8 sesame samples were tested, of which 4 (50.0%) harbored 4.3–5.6 log cfu/g standard plate count and 2.7–4.3 log cfu/g Enterobacteriaceae bacilli. Pantoea dispersa, P. septica and P. agglomerans were identified by a MALDI-TOF MSbased test. One (12.5%) sample harbored 3.6 log cfu/g Bacillus cereus, but this strain lacked the …

Study Of The Mechanism Of Fomes Officinalis Polysaccharides Inhibiting The Contraction Of The Isolated Duodenum In Mice Through The Myenteric Plexus, Ailong Sha, Haiyan Hao

The Thai Journal of Veterinary Medicine

The objectives of this study were to observe the effect of different concentrations of Fomes officinalis polysaccharides (FOPs) on the contractile activity of the isolated duodenal smooth muscle in mice and explore its mechanism. The contractility of the isolated duodenum was recorded by the Medlab biological signal acquisition and processing system before and after administration and the effects of FOPs-treated groups at low, medium and high dose (5, 10 and 20 mg/mL) on the contractile frequency and amplitude of the intestines were observed. Adrenaline hydrochloride (AD) and CaCl2 were selected respectively to be co-incubated with the high dose FOPs to …

Evaluation Of Tear Quantity And Quality Treated With Gintonin Eye Drops In Labrador Retrievers And English Springer Spaniels, Ji-Seung Choi, Joon-Young Kim, Soon-Wuk Jeong

The Thai Journal of Veterinary Medicine

The aims of this study were to determine the relationship between tear osmolarity (TO), Schirmer tear test-1 (STT- 1) and tear film break-up time (TBUT) values in canines and to determine the impact of eye drops containing gintonin - a ginseng-derived lysophosphatidic acid (LPA) receptor ligand - on these indices. Fourteen canines (four English springer spaniels and ten Labrador retrievers) were randomized into two groups: the control group received only placebo eye drops (carboxymethylcellulose), while the experimental group received carboxymethylcellulose eye drops containing 5 µg of gintonin. Treatment was applied twice per day for a period of four weeks and …