Comparative Evaluation Of Indirect Enzyme Linked Immunosorbent Assay, Rose Bengal Plate Test, Microagglutination Test, And Polymerase Chain Reaction For Diagnosis Of Brucellosis In Buffaloes, Malik Raies Ul Islam, Mohinder Pratap Gupta, Pritam Kaur Sidhu, Gursimran Filia, Hari Mohan Saxena, Tawheed Ahmed Shafi
Turkish Journal of Veterinary & Animal Sciences
In the present study, 178 blood samples from buffaloes were tested against indirect enzyme linked immunosorbent assay (I-ELISA), rose bengal plate test (RBPT), microagglutination test (MAT), modified microagglutination test (mMAT), and polymerase chain reaction (PCR) to select the most suitable test for efficient and effective diagnosis of bovine brucellosis. Win Episcope 2 software was used to determine the agreement between tests (kappa values at 95% confidence interval). I-ELISA was pair compared with all the other tests. Out of 178 samples, 102 were found positive by I-ELISA, 81 by RBPT, 85 by MAT, 79 by mMAT, and 68 by PCR. Substantial …
Scrapie Cases In The Northern Cyprus,
2013
TÜBİTAK
Scrapie Cases In The Northern Cyprus, Aydin Gürel, Ahmet Gülçubuk, Nuri̇ Turan, Christopher Richard Helps, Hüseyi̇n Yilmaz
Turkish Journal of Veterinary & Animal Sciences
Scrapie is a fatal neurodegenerative disease affecting small ruminants that causes economic losses in many countries. It is categorized together with other fatal neurodegenerative diseases as a transmissible spongiform encephalopathy. The aim of this study was to investigate clinical cases and the cause of death in a flock of sheep from Northern Cyprus (Turkish Republic of Northern Cyprus) suspected of having scrapie. Brains from 4 sheep showing clinical signs of classical scrapie, out of a flock of 200 animals, were analyzed by histopathological examination and infective prion enzyme-linked immunosorbent assay (ELISA). The 4 affected sheep were 2-3 years of age …
Biocompatibility Of High-Density Porous Polyethylene Covered With Fascia Lata In Dorsal Nasal Augmentation-An Experimental Study In Rabbits, Güven Yildirim, Hasan Alpak, İbrahi̇m Sayin, Vedat Onar
Turkish Journal of Veterinary & Animal Sciences
Ten New Zealand Albino rabbits were used in this study. A lateral incision was made on the nasal dorsum and a pocket formed in the subperiosteal plane to replace the implant. A fascia lata graft was obtained from the regio femoralis. High-density porous polyethylene (HDPP) was cut and covered with fascia lata. The material was positioned in the pocket formed on the nasal dorsum. Four months post-procedure, magnetic resonance imaging (MRI) was performed to evaluate the resorption of the material. Animals were then sacrificed and the nasal dorsum, including the graft material, was removed and subjected to macroscopic and histopathological …
Epididymitis And Orchitis Caused By Brucella Melitensis Biovar 3 In A Merino Ram,
2013
TÜBİTAK
Epididymitis And Orchitis Caused By Brucella Melitensis Biovar 3 In A Merino Ram, Esra Büyükcangaz, Ayli̇n Alasonyalilar Demi̇rer, Sevi̇l Erdenli̇ğ, Sabi̇re Deni̇z Misirlioğlu
Turkish Journal of Veterinary & Animal Sciences
This paper describes a case of fibrinopurulent epididymitis and orchitis in a Merino ram owing to Brucella melitensis biovar 3, detected by histopathology, bacteriology, and PCR. Gross lesions included enlargement of the scrotum by abundant fibrinous exudate and adhesions between testicular tunics. Histopathologic lesions were characterized by epididymal abscesses and testicular atrophy. B. melitensis was cultured from the exudate as well as the internal organs of the ram. After conventional biotyping procedures were applied, the isolate was identified as B. melitensis biovar 3. The isolate was also confirmed as Brucella spp. by genus-specific PCR.
Factors Affecting Colostrum Quality Of Ewes And Immunostimulation,
2013
TÜBİTAK
Factors Affecting Colostrum Quality Of Ewes And Immunostimulation, Özge Yilmaz, Güven Kaşikçi
Turkish Journal of Veterinary & Animal Sciences
Maternal antibodies play a major role in the development of the immune system of a young animal. Ideally, the transmission of maternal immunity to the foetus occurs in the uterus. However, in ruminants, as there is no maternal antibody transfer from the mother to her young by way of the uterus, the antibodies present in the colostrum are a vital necessity for newborn ruminants. After birth, an unhealthy neonatal period for the young animal and an unhealthy puerperal period for the mother can cause significant financial losses. In animal husbandry, due to the budget allocated for the treatment of diseases …
Ultrasonographic Evaluation Of Buffalo Eyes,
2013
TÜBİTAK
Ultrasonographic Evaluation Of Buffalo Eyes, Gholamreza Assadnassab, Majid Fartashvand
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to describe the ultrasonography appearance and measurements of normal buffalo eyes. Ocular ultrasonographic examinations were performed on 20 adult buffaloes. Transpalpebral ultrasonographic images were obtained with a 6-8 MHz linear transducer in the axial imaging plane. The ultrasonographic appearance of structures within the buffalo eye is similar to that in other species. When comparing measurements, the anteroposterior depth of the lens, vitreous chamber, and axial length of the globe on the left side were greater than on right, and the anterior chamber depth and scleroretinal rim wall thickness on the left side were also …
The Effect Of Flashed Light On The Ultrastructure Of The Retinal Photoreceptor Cells In Rabbit: A Transmission Electron Microscope Study, Arash Esfandiari, Iraj Posti, Asghar Dehghan, Nasser Hadjipour
Turkish Journal of Veterinary & Animal Sciences
The effects of light exposure on the retina have been carefully considered in recent years. The purpose of the current study was to investigate the effect of flashed light with low and high intensity on the photoreceptor layer of rabbit. Fifteen adult male New Zealand White rabbits were randomly divided into 3 groups: a control group, experimental group I (exposed to 60 W of flashed light 5 times), and experimental group II (exposed to 1000 W of flashed light 5 times). The retinas of the eyes were removed and studied by transmission electron microscope. The photoreceptor layer damage in experimental …
The Effect Of Electron Beam Irradiation On Β-Glucan Content, X-Ray Diffraction Of Starch, Protein Subunit Patterns, And In Vivo Digestibility Of Barley Grain In Cockerels, Parvin Shawrang, Ali Asghar Sadeghi, Behnam Ghorbani
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to evaluate the effect of electron beam radiation on the chemical composition, the \beta-glucan content, the water extract viscosity, the crystallinity of starch, the patterns of protein subunits, and the in vivo digestibility of barley grain. Barley samples were irradiated at doses of 10, 15, 20, 25, and 30 kGy under the electron beam. In a completely randomized design, cockerels were fed raw or irradiated samples to determine the digestibility of crude protein and gross energy. Irradiation had no effect on the total contents of protein, starch, and \beta-glucan. At doses of 10, 15, 20, …
Fertility Issues In Simmental Cows In Central Croatia: A 5-Year Study,
2013
TÜBİTAK
Fertility Issues In Simmental Cows In Central Croatia: A 5-Year Study, Robert Zobel, Suzana Tkalcic, Wendell Cole
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to establish the fertility trends in dairy cows in Central Croatia and compare the trends and causes of infertility and subfertility during a 5-year period. A total of 12,060 Simmental cows and heifers were included in the study. The animals were examined by vaginoscopy, transrectal palpation, and ultrasonography on a regular basis. Fertility problems that included infertility and subfertility were diagnosed in 56.61% of all observed animals. The total number of animals diagnosed with subfertility increased and almost doubled by the end of the study period. Subfertility issues associated with anovulatory oestrus, delayed ovulation, …
Relationship Between Dead Pupa Removal And Season And Productivity Of Honey Bee (Apis Mellifera, Hymenoptera: Apidae) Colonies,
2013
TÜBİTAK
Relationship Between Dead Pupa Removal And Season And Productivity Of Honey Bee (Apis Mellifera, Hymenoptera: Apidae) Colonies, Ahmet Güler, Hakan Toy
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to evaluate the dead pupae removal behaviour of 90 honey bee (Apis mellifera L.) colonies during the beekeeping season and the relationships between removal and colony productivity. The liquid nitrogen technique was used in May, June, July, August, and September. The number of removed pupae was significantly (P < 0.01) correlated with the month and changed throughout the season. The highest cleaning efficiency of the colonies were recorded in July and September, and the lowest were in May and August. While the average dead pupae removal was 83.75% in the 90 colonies, the percentage of removed pupae varied between 56.4% and 99.3% during the 5 months. There were significant positive correlations between dead pupae removal and honey yield (P < 0.01; r = 0.295), bee wax production (P < 0.01; r = 0.334), and adult worker bee population (P < 0.05; r = 0.233). No correlation was found between dead pupae removal and brood production and average temperature. Although hygienic behaviour has positive effects on many characters relating to productivity in untreated breeding populations, it is affected by many biotic and abiotic factors. This behaviour decreases with many stressful conditions (wasps, Merops sp., predators, honey harvesting) and increases with colony strength during the season.
Effect Of Corpus Luteum: Quality And Recovery Rate Of Buffalo (Bubalus Bubalis) Oocytes,
2013
TÜBİTAK
Effect Of Corpus Luteum: Quality And Recovery Rate Of Buffalo (Bubalus Bubalis) Oocytes, Lakshman Sahoo, Suresh K. Singla
Turkish Journal of Veterinary & Animal Sciences
Recovery rate and quality of oocytes were investigated from abattoir collected ovaries with and without corpora lutea (CL). The oocytes were categorized into four grades: grade-A, grade-B, grade-C, and grade-D based on the presence of the cumulus cells complex around the oocytes. The number of oocytes per ovary were 1.7 ±0.1 and 1.5 ±0.1, obtained from ovaries without CL and with CL, respectively. Recovery rate of different grades of oocytes from ovaries with CL and without CL were almost identical.
Relative Transit Time Of Chyme Between Duodenal And Jejunal Segments Of The Small Intestine Of Cattle,
2013
TÜBİTAK
Relative Transit Time Of Chyme Between Duodenal And Jejunal Segments Of The Small Intestine Of Cattle, Alejandro Plascencia, Maria Alejandra Lopez-Soto, Victor Manuel Gonzalez, Francisco Javier Monge, Richard Zinn
Turkish Journal of Veterinary & Animal Sciences
Two steers (228 ± 4.5 kg) equipped with \"T\" type cannulas in the proximal duodenum, duodenal-jejunal juncture, and distal ileum were used to measure transit time of chyme within the duodenum and jejunum. Steers were fed (as fed basis) with 5.75 kg of chopped alfalfa hay per day. The transit time was measured during 3 consecutive days using aniline dye, which was pulse-dosed via the duodenal and jejunal cannulas. The site of each cannula placement was confirmed and the small intestine was dissected and measured. Time required between the infusion of aniline dye into the proximal duodenal cannula and its …
Vertical Transmission Of Porcine Circovirus 2b (Pcv2b) To Mouse Fetuses In Maternal Uterus By Artificial Insemination With Semen From Pcv2b-Infected Male Mouse, Deng Zhibang, Gong Qianlong, Wang Naidong, Yuan Anwen, Yu Xing Long
Turkish Journal of Veterinary & Animal Sciences
In order to investigate whether porcine circovirus 2b (PCV2b) can be replicated in the spermatids of mice and whether semen from PCV2b-infected male mice can induce reproductive failure in female mice, 10 male Kunming mice were intraperitoneally inoculated with PCV2b, and 10 female Kunming mice were artificial inseminated with semen from the PCV2b-inoculated male mice. Semen from inoculated male mice and newborn mice or dead fetuses from inseminated female mice were tested for PCV2b by polymerase chain reaction, transmission electron microscopy, and immunofluorescent histochemistry. The results showed that PCV2b was capable of being replicated in spermatids in Kunming mice and …
Fungal Peritonitis In Dog Caused By Candida Albicans - A Case Report And Literature Overview,
2013
TÜBİTAK
Fungal Peritonitis In Dog Caused By Candida Albicans - A Case Report And Literature Overview, Kamila Glinska, Marcin Jankowski, Krzysztof Kubiak, Jolanta Spuzak, Maciej Grzegory, Stanislaw Dzimira
Turkish Journal of Veterinary & Animal Sciences
This clinical case concerns a male dog of Pointer breed, 10 years of age. The clinical examination revealed severe tenderness and a tension in the abdominal walls. The ultrasound examination detected fluid in the peritoneal cavity. The fluid collected from the peritoneal cavity displayed typical features of exudative fluid. In the cytological examination of the fluid, numerous stimulated endothelial cells, lymphocytes, and neutrophils were found. In the microbiological examination of the peritoneal fluid, Candida albicans was cultured. On the basis of the clinical signs, laboratory test results of the peritoneal fluid, and microbiological culture, the diagnosis of fungal peritonitis was …
The Use Of Moringa Oleifera In Poultry Diets,
2013
TÜBİTAK
The Use Of Moringa Oleifera In Poultry Diets, Talha E. Abbas
Turkish Journal of Veterinary & Animal Sciences
Poultry production sectors in developing countries are facing some problems, one of which is an increase in the cost of feed due to high prices of protein and energy sources. In addition, they are also faced with the problem of the development of antibiotic-resistant pathogens due to unwise and excessive use of antibiotics. Researchers are therefore looking for cheap, available, and safe alternative sources of protein and energy. In addition, scientists are also searching for natural antimicrobial ingredients. Some tropical legumes and plants were introduced into poultry diets as protein sources to decrease the cost of the feed. Recent studies …
Polymorphism Of 5’ Regulatory Region Of Caprine Fshr Gene And Its Association With Litter Size In Jining Grey Goat,
2013
TÜBİTAK
Polymorphism Of 5’ Regulatory Region Of Caprine Fshr Gene And Its Association With Litter Size In Jining Grey Goat, Xiaohong Guo, Yang Li, Mingxing Chu, Chunjing Feng, Ran Di, Qiuyue Liu, Tao Feng, Guiling Cao, Dongwei Huang, Li Fang, Qianqian Tang, Yuehui Ma, Kui Li
Turkish Journal of Veterinary & Animal Sciences
Single nucleotide polymorphisms of the 5' regulatory region of the FSHR gene were detected in 3 goat breeds (Jining Grey, Boer, and Inner Mongolia Cashmere) by the polymerase chain reaction-single-strand conformation polymorphism method, and its effects on litter size in Jining Grey goats were also evaluated. Concerning primer P1, 2 genotypes (AA and BB) were detected in Boer goats, while only the AA genotype was identified in Jining Grey and Inner Mongolia Cashmere goats. Sequencing revealed a T->A transversion at the 26th position and an A->C transversion at the 61st position of the amplified region in genotype BB …
Phenotypic And Genotypic Characterization Of Antibiotic-Resistant Soil And Manure Bacteria Adjacent To Swine Production Facilities, Meli̇ke Eki̇zoğlu, Satoshi Koike, Ivan Krapac, Mahmut Nedi̇m Sultan, Roderick Mackie
Turkish Journal of Veterinary & Animal Sciences
This study aimed to identify phenotypic and genotypic resistance profiles of bacteria isolated from soil and manure samples obtained from a farm where tetracycline and tylosin were used extensively. Samples were collected from the manure and soil, before and after manure application. All of the 151 bacteria were identified based on BLAST results. Minimum inhibitory concentrations were determined by agar dilution and E-test methods. Class-specific primers were used to amplify 6 erythromycin and 7 tetracycline resistance (Tc^r) genes for 46 tetracycline- and 18 erythromycin-resistant isolates. Tc^r gene sequence identity between different species of isolates was identified to investigate the dissemination …
Enhancement Of Immune Response For Newcastle Disease Vaccine Using A Combined Adjuvant Solution Of Astragalus Polysaccharides, Levamisole, And Selenoprotein, Yaogui Sun, Xiaojing Xie, Junping He, Junbing Jiang, Ruiyan Niu, Yuansheng Bai, Hongquan Li
Turkish Journal of Veterinary & Animal Sciences
The Newcastle disease vaccine strain I (ND I vaccine) was reconstituted using a 3-component combined adjuvant solution containing Astragalus polysaccharide (APS), levamisole (LMS), and selenoprotein (Se). A total of 200 Hy-Line brown chickens, 60 days of age, were randomly assigned into 10 groups. The first group was a negative control and was immunized using the phosphate buffered saline-reconstituted vaccine. The other 9 groups were immunized using the vaccine reconstituted with the combined adjuvant solution. The serum and blood samples were collected on days 7, 14, 21, 27, 35, and 42 after the vaccinations and were analyzed for IgG, IL-2, and …
Comparative Evaluation Of Liquid-Phase Blocking Elisa And Solid-Phase Competition Elisa Methods For The Detection Of Antibodies To The Structural Proteins Of Foot-And-Mouth Disease Types O And A Viruses, Murat Şevi̇k, Fahri̇ Ferudun Öztürk
Turkish Journal of Veterinary & Animal Sciences
In this study, we compared 2 methods, liquid-phase blocking ELISA (LPBE) and solid-phase competition ELISA (SPCE), for the detection of antibodies to the structural proteins of foot-and-mouth disease virus (FMDV). These methods were compared using sera collected from cattle (n = 30) without a history of foot-and-mouth disease infection or vaccination, cattle (n = 180) vaccinated with oil-adjuvanted bivalent vaccine, and international reference sera (positive, weak positive, and negative) for FMDV serotypes O and A. The results showed that SPCE had a better specificity (96.67% for serotype O and 100% for serotype A) than LPBE (90% for serotype O and …
Acute Toxicity Of Diazinon And Its Effects On Hematological Parameters In The Indian Carp, Cirrhinus Mrigala (Hamilton),
2013
TÜBİTAK
Acute Toxicity Of Diazinon And Its Effects On Hematological Parameters In The Indian Carp, Cirrhinus Mrigala (Hamilton), Abdul Rauf, Naeemuddin Arain
Turkish Journal of Veterinary & Animal Sciences
Diazinon is a commonly used pesticide and its existence in the fresh water reservoirs of Pakistan has been reported by some authors. A semistatic experiment was conducted to find out the acute toxicity of diazinon as an aquatic pollutant on some hematological parameters of Indian carp (Cirrhinus mrigala). The 1, 24, 48, 72, and 96 h LC50 values of diazinon for Indian carp were estimated as 17.32 mg/L, 15.39 mg/L, 12.64 mg/L, 10.98 mg/L, and 8.15 mg/L respectively. A significant increase in the number of dead fish was observed as the concentration of diazinon and exposure time increased (P < 0.05). The results after acute exposure to 8.15 mg/L of diazinon for 96 h showed that compared to the control fish, the total erythrocytes, leukocytes, hemoglobin, and hematocrit values decreased significantly (P < 0.05) in exposed fish. The mean corpuscular volume and mean corpuscular hemoglobin increased significantly (P < 0.05), while the mean corpuscular hemoglobin concentration of both groups remained unchanged during the study. In conclusion, acute exposure to LC50 values of diazinon induced hematological alterations in Indian carp and offers a tool to evaluate toxicity derived alterations.
