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Intra-Articular Characteristics Of Dogs With Medial Patellar Luxation, Sethapong Jahrupatrakorn 2017 Faculty of Veterinary Science

Intra-Articular Characteristics Of Dogs With Medial Patellar Luxation, Sethapong Jahrupatrakorn

Chulalongkorn University Theses and Dissertations (Chula ETD)

The study investigated the relationship of degree of patellar luxation of dogs with joint radiography, intra-articular degenerative change of the stifle with medial patellar luxation (MPL), histopathologic change, and biomarker index. Thirty-eight stifles were divided into four groups: normal stifle joints (4), grades 2 (13), 3 (12), and 4 (9) MPL. Demographic data, lameness score, and degree of patellar luxation of all dogs were assessed and recorded. Radiographs were taken to assess osteophyte formation. Synovial tissue was collected for microscopic evaluation differential cell counts, and cytokines measurement. Stifle joints with MPL were scored during surgical correction. All observed data were …


Comparative Surgical Treatment Of Elongated Soft Palate In Brachycephalic Dogs Using The Ultrasonic Scalpel And The Conventional Incisional Techniques, Sunisa Thunyodom 2017 Faculty of Veterinary Science

Comparative Surgical Treatment Of Elongated Soft Palate In Brachycephalic Dogs Using The Ultrasonic Scalpel And The Conventional Incisional Techniques, Sunisa Thunyodom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Brachycephalic airway obstruction syndrome (BAOS) is life threatening condition in brachycephalic dogs. Surgical correction of primary disorders including elongated soft palate and stenotic nares is indicated in all cases. Aim of this study was to compare the use of the ultrasonic scalpel with the conventional incision technique for staphylectomy. Twenty dogs with BAOS enrolled in this study were randomly assigned into conventional (N=10) or ultrasonic group (N=10). Respiratory score was evaluated and recorded prior to surgery. Staphylectomy time and bleeding were recorded. Respiratory, postoperative complication and pain scores were evaluated and recorded at days 1, 3, 7, 14 and 28 …


การประเมินทางพันธุกรรมของลักษณะการอยู่รอดของลูกสุกรที่สัมพันธ์กับขนาดครอกและน้ำหนักแรกเกิดรายตัว, ทิตยา วรวัฒนธรรม 2017 คณะสัตวแพทยศาสตร์

การประเมินทางพันธุกรรมของลักษณะการอยู่รอดของลูกสุกรที่สัมพันธ์กับขนาดครอกและน้ำหนักแรกเกิดรายตัว, ทิตยา วรวัฒนธรรม

Chulalongkorn University Theses and Dissertations (Chula ETD)

ประเมินค่าพารามิเตอร์ทางพันธุกรรมของลักษณะการอยู่รอดของลูกสุกรแรกเกิด (PSB) และการอยู่รอดของลูกสุกรก่อนหย่านม (PSW) ร่วมกับลักษณะน้ำหนักแรกเกิดรายตัว (IBW) และลักษณะขนาดครอก (LS) จากข้อมูลการอยู่รอดของลูกสุกรจำนวน 14,389 บันทึก (1,413 ครอก) จากแม่สุกร 705 ตัว ข้อมูลการอยู่รอดของลูกสุกรพันธุ์แลนด์เรซ ยอร์กเชียร์ และดูร็อก บันทึกในฟาร์มที่เลี้ยงสุกรในโรงเรือนเปิดที่ไม่มีระบบระบายความร้อน เป็นข้อมูลของลูกสุกรที่เกิดระหว่างปี พ.ศ. 2549 ถึง 2560 ประมาณค่าองค์ประกอบความแปรปรวนด้วยวิธี Gibbs sampling สำหรับลักษณะแบบไบนารี่และวิธี Average Information Restricted Maximum Likelihood (AIREML) สำหรับลักษณะแบบต่อเนื่อง ผลการศึกษาพบว่า ลำดับท้อง ระยะเวลาการอุ้มท้องของแม่สุกร และกลุ่มของโรงเรือน-ปี-เดือนที่ลูกสุกรเกิด มีอิทธิพลต่อ PSB, PSW และ IBW อย่างมีนัยสำคัญทางสถิติ (p<0.05) นอกจากนี้ PSW และ IBW ยังได้รับอิทธิพลจากพันธุ์ และเพศของสุกร (p<0.05) สำหรับ LS พบว่าขึ้นอยู่กับอิทธิพลของพันธุ์ของลูกสุกร และกลุ่มของโรงเรือน-ปี-เดือนที่ลูกสุกรเกิด (p<0.05) ค่าอัตราพันธุกรรมของ PSB, PSW, IBW และ LS มีค่าเท่ากับ 0.23 ± 0.02, 0.36 ± 0.03, 0.38 ± 0.03 และ 0.22 ± 0.01 ตามลำดับ ค่าสหสัมพันธ์ทางพันธุกรรมระหว่าง IBW กับ PSB และ PSW มีค่าสูงเท่ากับ 0.77 ± 0.05 และ 0.85 ± 0.05 ตามลำดับ ในขณะที่ค่าสหสัมพันธ์ทางพันธุกรรมระหว่าง PSB กับ PSW มีค่าปานกลางเท่ากับ 0.69 ± 0.14 สำหรับลักษณะรายครอกพบว่าค่าสหสัมพันธ์ทางพันธุกรรมระหว่าง LS กับลักษณะเปอร์เซ็นต์การอยู่รอดของลูกสุกรแรกเกิด (%PSB) เปอร์เซ็นต์การอยู่รอดของลูกสุกรก่อนหย่านม (%PSW) และน้ำหนักแรกเกิดเฉลี่ย (ABW) มีค่าเป็นลบเท่ากับ -0.16 ± 0.03 -0.64 ± 0.02 และ -0.99 ± 0.001 ตามลำดับ จากค่าสหสัมพันธ์ทางพันธุกรรมแสดงให้เห็นว่า การคัดเลือกเพื่อเพิ่มน้ำหนักแรกเกิดของลูกสุกรอาจมีผลทำให้ PSB และ PSW เพิ่มขึ้น แต่หากคัดเลือกเพื่อเพิ่ม LS จะมีผลทำให้ %PSB, %PSW และ ABW ลดลง


The Lowdown Of Out-Of-Competition Testing: What The Rmtc Isn't Telling Us About Its Proposed Regulations, Clara Fenger, Tanya Boulmetis, Kim Brewer, Thomas Tobin 2016 University of Kentucky

The Lowdown Of Out-Of-Competition Testing: What The Rmtc Isn't Telling Us About Its Proposed Regulations, Clara Fenger, Tanya Boulmetis, Kim Brewer, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.


Allelic Variation In Cxcl16 Determines Cd3+ T Lymphocyte Susceptibility To Equine Arteritis Virus Infection And Establishment Of Long-Term Carrier State In The Stallion, Sanjay Sarkar, Ernest Bailey, Yun Young Go, R. Frank Cook, Ted Kalbfleisch, John E. Eberth, R. Lakshman Chelvarajan, Kathleen M. Shuck, Sergey Artiushin, Peter J. Timoney, Udeni B. R. Balasuriya 2016 University of Kentucky

Allelic Variation In Cxcl16 Determines Cd3+ T Lymphocyte Susceptibility To Equine Arteritis Virus Infection And Establishment Of Long-Term Carrier State In The Stallion, Sanjay Sarkar, Ernest Bailey, Yun Young Go, R. Frank Cook, Ted Kalbfleisch, John E. Eberth, R. Lakshman Chelvarajan, Kathleen M. Shuck, Sergey Artiushin, Peter J. Timoney, Udeni B. R. Balasuriya

Maxwell H. Gluck Equine Research Center Faculty Publications

Equine arteritis virus (EAV) is the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of horses and other equid species. Following natural infection, 10–70% of the infected stallions can become persistently infected and continue to shed EAV in their semen for periods ranging from several months to life. Recently, we reported that some stallions possess a subpopulation(s) of CD3+ T lymphocytes that are susceptible to in vitro EAV infection and that this phenotypic trait is associated with long-term carrier status following exposure to the virus. In contrast, stallions not possessing the CD3+ T …


Assessing Readiness For Clinical Practice: Students’ Perspectives Of Their Veterinary Curriculum, L. Chris Sanchez, Alison Kwiatkowski, Jeff Abbott, Dana N. Zimmel, Linda S. Behar-Horenstein 2016 University of Florida College of Veterinary Medicine

Assessing Readiness For Clinical Practice: Students’ Perspectives Of Their Veterinary Curriculum, L. Chris Sanchez, Alison Kwiatkowski, Jeff Abbott, Dana N. Zimmel, Linda S. Behar-Horenstein

The Qualitative Report

Studies describing the effectiveness of a veterinary curriculum from the student perspective are currently sparse. The overall purpose of this investigation was to describe students’ perceived preparedness for clinical practice. Three focus group meetings with fourth year veterinary students were conducted. Data were open-coded and categorized to identify themes. Four main themes emerged: Challenging communications, Un/appreciating curricular experiences, Documenting demands impede case involvement, and Hungering for timely, effective feedback. Overall students felt comfortable talking to clients about medicine but less comfortable discussing euthanasia or money; they appreciated the split clinical curriculum but questioned the value of the 1st/2nd year courses; …


Animal Health Matters, Jane Hennings, Larry Holler, Russ Daly, Linda Fawcett 2016 South Dakota State University

Animal Health Matters, Jane Hennings, Larry Holler, Russ Daly, Linda Fawcett

Animal Health MATTERS Newsletter

[Page] 1 SDSU and SHIC Study on Senecavirus Offers Keen Insights
[Page] 2 Director's Message: Innovation in the Laboratory and Being Proactive in the Protection of Animal Health
[Page] 2 Bovine Abortion-Sample Submission Guidelines
[Page] 3 South Dakota One Health Meeting Covers Bacterial Zoonotic Disease
[Page] 3 Small Ruminant, Poultry Serology Offerings Expanded at the ADRDL
[Page] 4 Molecular Diagnostics Section Evolves, Adds New Tests
[Page] 5 Recent PCR tests Added by ADRDL
[Page] 6 New Employees
[Page] 7 ADRDL Continues Their Critical Role Supporting Animal Exports


Evaluation Of Several Pre-Clinical Tools For Identifying Characteristics Associated With Limb Bone Fracture In Thoroughbred Racehorses, Anthony Nicholas Corsten 2016 Purdue University

Evaluation Of Several Pre-Clinical Tools For Identifying Characteristics Associated With Limb Bone Fracture In Thoroughbred Racehorses, Anthony Nicholas Corsten

Open Access Theses

Catastrophic skeletal fractures in racehorses are devastating not only to the animals, owners and trainers, but also to the perception of the sport in the public eye. The majority of these fatal accidents are unlikely to be due to chance, but are rather an end result failure from stress fractures. Stress fractures are overuse injuries resulting from an accumulation of bone tissue damage over time. Because stress fractures are pathological, it is possible that overt fractures can be predicted and prevented. In this study, third metacarpals (MC3) from 33 thoroughbred racehorse comprised of 8 non-fractured controls and 25 horses that …


2016 Study Of Current Conditions Of Kentucky County Animal Shelters And Degree Of Compliance With Kentucky Animal Shelter Laws, Cynthia L. Gaskill, Rachel Cullman-Clark, Liane Lachiewicz, Matt Lamarre, Brad Rohleder, Kristin Sadler, Rachel Sparling, Craig N. Carter 2016 University of Kentucky

2016 Study Of Current Conditions Of Kentucky County Animal Shelters And Degree Of Compliance With Kentucky Animal Shelter Laws, Cynthia L. Gaskill, Rachel Cullman-Clark, Liane Lachiewicz, Matt Lamarre, Brad Rohleder, Kristin Sadler, Rachel Sparling, Craig N. Carter

Veterinary Science Reports

Kentucky’s county animal shelter conditions have not been studied for over 20 years. Major goals of this study were to assess current conditions in Kentucky’s county shelters and determine the degree of compliance with Kentucky shelter laws. Additional information was gathered to determine the major problems and needs identified by shelter personnel and researchers. Data was used to determine if additional state funds or refinements and additions to current laws are warranted to ensure humane care of animals in Kentucky’s county shelters. Researchers consisted of a group of 6 veterinary students who traveled to all Kentucky county animal shelters. Ninety …


Chikungunya Virus Infection In Balb/C And Icr Mouse Models, Sonthaya Tiawsirisup, Ranida Tuanudom, Nichapat Yurayart 2016 Chulalongkorn University

Chikungunya Virus Infection In Balb/C And Icr Mouse Models, Sonthaya Tiawsirisup, Ranida Tuanudom, Nichapat Yurayart

The Thai Journal of Veterinary Medicine

Chikungunya virus (CHIKV) infection is an emerging or re-emerging infectious disease found in several countries. The ecology of this virus involves humans, reservoir animals, and mosquito vectors. Two experiments were conducted in this study. Blood samples were collected and tested for CHIKV infection by using immunocytochemistry (ICC) staining and reverse transcription polymerase chain reaction (RT-PCR). For the first experiment, BALB/c mice were inoculated with 106.3 CID50/mouse. Mean CHIKV titers in the BALB/c mice were 103.3, 101.5, 102.3, and 102.3 CID50/ml of serum on day 1 to day 4 post inoculation (PI), respectively. For the second experiment, there were two groups …


Regulation Of Alpha-Herpesvirus Reactivation From Latency By Stress, Insun Kook 2016 University of Nebraska-Lincoln

Regulation Of Alpha-Herpesvirus Reactivation From Latency By Stress, Insun Kook

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Bovine herpes virus 1 (BHV-1) and Herpes simplex virus 1 (HSV-1) are crucial etiological viral agent of clinical diseases. HSV-1 and BHV-1 establish latent infection in sensory neurons. Periodically, reactivation from latency occurs resulting in virus excretion and transmission. Stress increases corticosteroid levels and the incidence of HSV-1 and BHV-1 reactivation from latency. The synthetic corticosteroid, dexamethasone (DEX) mimics stress and induces BHV-1 and HSV-1 reactivation. However, molecular mechanisms by which corticosteroid mediates viral reactivation are not well understood. My dissertation has focused on elucidating events that induce BHV- 1 or HSV-1 reactivation during the early stages of stress-induced escape …


The Impact Of Tickling Rats On Human-Animal Interactions And Rat Welfare, Megan Renee LaFollette 2016 Purdue University

The Impact Of Tickling Rats On Human-Animal Interactions And Rat Welfare, Megan Renee Lafollette

Open Access Theses

Rats initially fear humans which can lead to negative affect, poor welfare, and difficult handling. Also, modeling and measuring positive affect states in rats can pose an additional challenge. Heterospecific play, or “tickling,” is a handling habituation technique that mimics rat rough-and-tumble play that is being used to study positive affect. It can also be used to reduce fear of human, improve welfare, and elicit a positive affect state. However, current studies implementing the technique in laboratory rats use a wide variety of protocols to achieve differential results. Unlike in laboratory environment, pet store rats experience high levels of novelty …


Challenges And Prospects Of The Embryo Transfer Industry From A European Standpoint, Michel Thibier 2016 Chulalongkorn University

Challenges And Prospects Of The Embryo Transfer Industry From A European Standpoint, Michel Thibier

The Thai Journal of Veterinary Medicine

In vivo derived (IVD) and in vitro produced (IVP) embryo transfers are respectively the 2nd and 3rd generations of the reproductive biotechnologies. Commercial application of these technologies began in the seventies for the former and in the eighties for the latter. After 40 years, they have clearly proved their sustainability to the livestock industry. The present article deals with bovine embryos only. The numbers of embryos collected or produced and transferred worldwide are reported in the first part. About one million bovine embryos are transferred each year. This clearly shows the significant impact they have on the cattle industry. The …


Advanced Biotechnologies For Wildlife Fertility Preservation, Pierre Comizzoli 2016 Chulalongkorn University

Advanced Biotechnologies For Wildlife Fertility Preservation, Pierre Comizzoli

The Thai Journal of Veterinary Medicine

Reproductive biotechnologies are critical tools for saving and maintaining endangered species. Some successes have been reported with the use and integration of artificial insemination (with fresh or frozen-thawed semen) in conservation programs. However, not a single species is currently managed through oocyte freezing or embryo-based technologies. This is primarily due to the lack of knowledge of species biology, as well as inadequate facilities, space, expertise, and funding needed for their successful application. More fundamental studies of animal reproductive biology as well as more fertility preservation options are needed with all parties involved (reproductive technologists, zoo biologists and conservationists) adopting parallel …


Semi-Automated Microbiopsy Device: A Potential Tool For Muscle Sampling In Horse, Metha Chanda, Ratchakrit Srikuea, Pawinee Piyachaturawat 2016 Chulalongkorn University

Semi-Automated Microbiopsy Device: A Potential Tool For Muscle Sampling In Horse, Metha Chanda, Ratchakrit Srikuea, Pawinee Piyachaturawat

The Thai Journal of Veterinary Medicine

Muscle biopsy is considered as a gold standard for evaluations of muscle integrity and pathological conditions in horse skeletal muscle. A semi-automated microbiopsy device with small needle has recently been introduced in horse muscle research, however there is no report on the quality of the muscle tissue obtained by the device. The aim of the present study was to examine the potential of the semi-automated microbiopsy device for horse skeletal muscle tissue sampling by evaluating the quality of samples for histological and biochemical analyses. Gluteus medius muscle samples were taken from six mature polo ponies and three ageing polo ponies …


Optimized Codons Of Polar Tube Protein 1 Gene Of Encephalitozoon Cuniculi To Enhance Protein Expression In Escherichia Coli, Waraporn Sinsuwonkwat, Pornchai Sanyathitiseree, Sakuna Phattanakunanan, Siriluk Jala, Preeda Lertwatcharasarakul 2016 Chulalongkorn University

Optimized Codons Of Polar Tube Protein 1 Gene Of Encephalitozoon Cuniculi To Enhance Protein Expression In Escherichia Coli, Waraporn Sinsuwonkwat, Pornchai Sanyathitiseree, Sakuna Phattanakunanan, Siriluk Jala, Preeda Lertwatcharasarakul

The Thai Journal of Veterinary Medicine

Encephalitozoon cuniculi (E. cuniculi) is an intracellular zoonotic parasite that is recognized as a common parasite of rabbits. Infected rabbits show neurological and renal disorder. The polar tube protein 1 of the spore is revealed to be antigenicity. The present study aimed to produce a recombinant polar tube protein 1 (rPTP1) for which the codon usage of a native ptp1 gene was optimized and synthesized for protein production in Escherichia coli (E. coli). Both a native ptp1 gene amplified by PCR technique and an optimized ptp1 gene were ligated into a pQE30 expression vector and transformed into an E. coli …


Modulatory Effects Of Pg102 (Actinidia Arguta) On Inflammatory Cytokine Mrna Expression In Canine Atopic Dermatitis, Yong-Uk Lee, Seol-Hee Park, Eui-Hwa Nam, Tae-Ho Chung, Cheol-Yong Hwang 2016 Chulalongkorn University

Modulatory Effects Of Pg102 (Actinidia Arguta) On Inflammatory Cytokine Mrna Expression In Canine Atopic Dermatitis, Yong-Uk Lee, Seol-Hee Park, Eui-Hwa Nam, Tae-Ho Chung, Cheol-Yong Hwang

The Thai Journal of Veterinary Medicine

Canine atopic dermatitis (AD) is a common, chronic relapsing inflammatory skin disease. An aqueous extract, PG102, derived from Actinidia arguta fruit, has been investigated as an anti-inflammatory agent in humans and atopy-like animal models, but its underlying mechanism for canine AD treatment has not been fully understood. This study evaluated the in vitro anti-inflammatory effect of PG102 with respect to the representative allergy-related cytokine panel from dust mite allergen extract (DME)-stimulated peripheral blood mononuclear cells (PBMCs) of canine AD patients using quantitative real-time PCR. PBMCs were isolated from eight dogs with definite diagnoses of AD and stimulated with DME for …


Osteogenic Differentiation Potential Of Canine Bone Marrow-Derived Mesenchymal Stem Cells Under Different Β-Glycerophosphate Concentrations In Vitro, Chenphop Sawangmake, Sirirat Nantavisai, Thanaphum Osathanon, Prasit Pavasant 2016 Chulalongkorn University

Osteogenic Differentiation Potential Of Canine Bone Marrow-Derived Mesenchymal Stem Cells Under Different Β-Glycerophosphate Concentrations In Vitro, Chenphop Sawangmake, Sirirat Nantavisai, Thanaphum Osathanon, Prasit Pavasant

The Thai Journal of Veterinary Medicine

Stem cell-based therapy employing bone marrow-derived mesenchymal stem cells has been proposed as a promising approach for bone regenerative treatment. Despite numerous studies supporting the application in human medicine, such information in veterinary practice is still lacking. Thus, this study aimed to investigate in vitro osteogenic differentiation potential of canine bone marrow-derived mesenchymal stem cells under different doses of β-glycerophosphate supplement. The isolated cells showed self-renewing ability and expressed pluripotent marker genes, zinc finger protein 42 and octamer-binding transcription factor 4, suggesting their potentiality in vitro. In vitro osteogenic differentiation was performed by using a regular osteogenic induction medium containing …


Inducible Vancomycin Resistance Is Common In Porcine Enterococcus Gallinarum And E. Casseliflavus Isolates, Wanida Tharvornvee, Chantima Pruksakorn, Porntippa Lekcharoensuk 2016 Chulalongkorn University

Inducible Vancomycin Resistance Is Common In Porcine Enterococcus Gallinarum And E. Casseliflavus Isolates, Wanida Tharvornvee, Chantima Pruksakorn, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

Enterococcus gallinarum and E. casseliflavus possess intrinsic vancomycin resistance, which is mediated by vanC-1 and vanC-2 gene clusters, respectively. The VanC resistance phenotype is expressed inducibly or constitutively. This study characterized the expression types for vancomycin resistance in 23 porcine E. gallinarum and 19 porcine E. casseliflavus isolates with vancomycin minimum inhibitory concentrations between 4 and 16 μg/ml. The vanSC and vanSC-2 genes were sequenced and the deduced amino acid sequences were compared to those of the inducible and constitutive resistance reference strains. Analysis of growth curves revealed that the resistance phenotype in all isolates was induced by vancomycin. In …


Field Study Of Seroconversion Of Three Different Commercial Vaccines Of Chicken Infectious Anemia Virus In Thailand, Niwat Chansiripornchai 2016 Chulalongkorn University

Field Study Of Seroconversion Of Three Different Commercial Vaccines Of Chicken Infectious Anemia Virus In Thailand, Niwat Chansiripornchai

The Thai Journal of Veterinary Medicine

The aim of this study was to evaluate the effects of different chicken infectious anemia virus (CIAV) vaccine strains in breeders and progeny under field conditions. For this experiment, one commercial poultry company was selected, and independent management for each house was applied to avoid vaccine cross contamination. No natural infection was found before vaccination. BioChek ELISA kits were used, and a minimum of 20 birds per flock were sampled in each sampling time. Three commercial CIAV vaccine strains that are available in Thailand were selected for the study: 26P4 was vaccinated subcutaneously, Cux-1 was vaccinated orally, and Del Ros …


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