Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus,
2017
TÜBİTAK
Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus, Ahmet Ceylan, Belma Alabay
Turkish Journal of Veterinary & Animal Sciences
The thymus is a primary lymphoid organ that provides the microenvironment required for the development of T lymphocytes. Thymic epithelial cells (TECs) are among the most critical components in the thymic microenvironment supporting thymocyte selection and maturation. Apoptosis is primarily a physiologic process that plays a major role during the development of T lymphocytes. The first purpose of this study was to examine morphological changes in apoptotic thymocytes by transmission electron microscope in piglets. The second purpose was to reveal the morphological typology of TECs in piglets. Tissue samples of the thymus were taken from healthy Yorkshire race pigs of …
Effect Of Utilization Of A Controlled-Release Intraruminal Monensin Device On Drymatter Intake, Milk Yield, And Some Reproductive Parameters In Dairy Cows, Seçki̇n Salar, Oğuz Çalişici, Duygu Çalişici, İdi̇l Baştan, Ayhan Baştan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to examine the effect of the prepartal administration of the monensin release capsule on dry matter intake (DMI), milk yield, and some reproductive parameters of dairy cows. The cows were divided into study (n = 35) and control (n = 41) groups. The study group cows were administered the intraruminal-monensin releasing capsules at 21 $\pm$ 3 days before expected calving and the control group cows were not subjected to treatment. Monthly mean DMI; cumulative milk yield at 30, 45, and 60 days in milk (DIM); peak milk yield; days of 1st AI; days open; …
Development Of Leptospiral Outer Membrane Protein-Based Dot-Elisa Assays And Immunoprofiling For Diagnosis Of Acute Canine Leptospirosis, Vamshi Krishna Sriram, Siju Joseph, Ambily Ramachandran, Mini Mangattumuruppel
Turkish Journal of Veterinary & Animal Sciences
The present study was carried out to evaluate outer membrane protein (OMP)-based relatively simple dot-ELISA assays for the diagnosis of acute canine leptospirosis and to identify the differential expression of leptospiral OMPs for identifying the markers of acute infection, if any. Among 91 canine serum samples screened, 63 samples were found to be positive by MAT. All the samples were tested by OMP-based IgM and IgG dot ELISAs. The sensitivities of IgM and IgG dot ELISA in comparison to MAT (titer: ≥1:400) were found to be 100%, whereas the specificities were found to be 89.71% and 45.31%, respectively. IgM and …
Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits,
2017
TÜBİTAK
Effect Of Polymorphisms In Intron 1 Of The Swine Pou1f1gene On Growth And Reproductive Traits, Lyubov Getmantseva, Anatoly Kolosov, Maria Leonova, Aleksander Usatov, Faridun Bakoev, Aleksander Klimenko, Siroj Bakoev
Turkish Journal of Veterinary & Animal Sciences
The most prospective and actual trend in farm animal breeding is studying gene polymorphisms affecting the productive traits. The objective of this investigation was to study the effect of polymorphisms in intron 1 of the swine POU1F1 gene on number of days to 100 kg (days to 100 kg), length of body (LB), backfat thickness (BF), and number of piglets born alive (NBA). Research was conducted on purebred pigs of Landrace (n = 80) and Duroc (n = 100) and crossbred pigs (Landrace x Large White) (n = 192). Insertions/deletions in intron 1 of the POU1F1 gene were defined by …
The Effect Of Combining Vitamin E And C On The Viability Improvement Of Transfected Ovine Spermatogonial Stem Cells After Cryopreservation And Thawing, Hosein Shabani, Mohammad Zandi, Hamideh Ofogi, Mohammad Reza Sanjabi, Khosro Hoseini Pajooh
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to identify whether vitamin E and C and their combination in basal freezing medium improved the viability of postthaw transfected spermatogonial stem cells (SSCs). For this purpose, SSCs were harvested by enzymatic digestion twice. After enrichment and culture on Sertoli cell feeder layer, SSCs were characterized by using alkaline phosphatase staining and expression of oct-4 and c-kit genes as specific stem cell markers. As to the transfection of SSCs, different concentrations of DNA (0.2, 0.4, and 0.8 μg) and turbofect (0.4 and 0.8 μL) in 100 μL of medium were studied. The results showed that cryopreservation …
Gene Cloning And Expression Analysis Of Mitochondrial Glutathione Reductase From Arabian Camel (Camelus Dromedarius) Liver In Escherichia Coli, Mona Alharbi, Abdulrahman Mohammad Alsenaidy, Sooad Aldaihan, Anwar Ahmed
Turkish Journal of Veterinary & Animal Sciences
Glutathione reductase (GR) is highly conserved among diverse taxa and has important biochemical functions. These functions may facilitate survival in harsh conditions, but the role of GR from the liver of the Arabian camel (Camelus dromedarius) is unknown. In this study, the mitochondrial glutathione reductase gene (Gsr) from C. dromedarius liver was cloned and highly expressed in Escherichia coli (Jm109) to gain insight into GR functions in the liver. After amplification of the cDNA encoding the functional unit of Gsr (1.2 kb), the products were cloned into the PGEM-T Easy and PET28a vectors. Gsr expression was confirmed using an immunoblotting …
Effects Of Prepartum Treatment With Monensin Or Propylene Glycol Mixed With Concentrate On Milk Yield And Blood Nefa And Bhba Levels In Dairy Cows, Zafer Meci̇toğlu, Sevi̇m Kasap, Mehmet Levent Özdüven, Muhi̇tti̇n Özder, Engi̇n Kennerman
Turkish Journal of Veterinary & Animal Sciences
Sixty multiparous Holstein-Friesian dairy cows made up the study sample. Group M (n = 20) received 300 mg/day monensin (Kexxtone, Elanco) for 21 days prior to the expected calving date. Group PGC (n = 20) received propylene glycol, mixed with concentrate and fed separately from total mixed ration (TMR) twice a day in the last 21 days of gestation. The control group, Group C (n = 20) did not receive any treatment prior to parturition. Cows in all three groups received PG (300 mL/day) for 5 days after parturition. Blood samples were collected 21 and 7 days prior to the …
Infectious Epididymitis Caused By Brucella Ovis In Croatian Sheep Flocks,
2017
TÜBİTAK
Infectious Epididymitis Caused By Brucella Ovis In Croatian Sheep Flocks, Zeljko Cvetnic, Maja Zdelar-Tuk, Sanja Duvnjak, Miroslav Benic, Zeljko Mihaljevic, Boris Habrun, Irena Reil, Marija Cvetnic, Silvio Spicic
Turkish Journal of Veterinary & Animal Sciences
The distribution of infection by the species Brucella ovis in rams and abortion cases in sheep in Croatia was investigated in the period from 2011 to 2015. The investigation relied on serological testing in rams and sheep after abortion conducted using a complement fixation test and immunosorbent assay conducted with bacteriological testing of the testes in seropositive rams and aborted material. Seropositive reactions were confirmed in 1100 (6%) of rams samples and in 12 (2.3%) of ewe samples. Isolated strains were also confirmed on the molecular level. In 10 (45.5%) animals asymmetry of the scrotum and unilateral enlargement of the …
Promising Synergistic Interactions Among Antimicrobials Against Multidrug-Resistant Escherichia Coli Isolated From Animals,
2017
TÜBİTAK
Promising Synergistic Interactions Among Antimicrobials Against Multidrug-Resistant Escherichia Coli Isolated From Animals, Pinar Şahi̇ntürk, Murat Cengi̇z
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
First Report Of Brucella Suis Biovar 2 In Outdoor Reared Pigs(Sus Scrofa Domesticus) In Serbia,
2017
TÜBİTAK
First Report Of Brucella Suis Biovar 2 In Outdoor Reared Pigs(Sus Scrofa Domesticus) In Serbia, Jadranka Zutic, Djordje Cvetojevic, Ljubisa Veljovic, Oliver Radanovic, Slobodan Stanojevic, Branislav Kureljusic
Turkish Journal of Veterinary & Animal Sciences
The objective of this work was to present the first case of Brucella suis biovar 2 isolation in outdoor reared pigs in Serbia. B. suis biovar 2 has not previously been detected in Serbia, from either wild boar or outdoor reared pigs. In our case, brucellosis was discovered in the region of Srem, which geographically constitutes a part of northwestern Serbia. Outdoor reared pigs in the Srem region are part of an extensive breeding system in the field and woods. In the course of a random visit to a herd in the above-mentioned area, the animals' owner discovered two aborted …
Molecular Types And Antimicrobial Resistance Profile Of Staphylococcus Aureus Isolated From Dairy Cows And Farm Environments,
2017
TÜBİTAK
Molecular Types And Antimicrobial Resistance Profile Of Staphylococcus Aureus Isolated From Dairy Cows And Farm Environments, Dattatray Gandhale, Rahul Kolhe, Saba Nalband, Padmakar Deshpande, Urmila Jagtap, Chandrakant Dhandore, Sujata Bhave, Sameer Jadhav, Dushyant Muglikar, Smita Kolhe
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to assess the prevalence of Staphylococcus aureus in cows with subclinical mastitis and detect enterotoxin genes and patterns of antimicrobial resistance. Out of 180 animals, subclinical mastitis was detected in 78 cows (46.99%) and three buffalo (21.43%). The prevalence of clinical mastitis was low (3.61%). S. aureus could be isolated from mastitic milk (67/81), udder swabs (38/81), floor swabs (13/27), milking machine liner (5/8), and healthy animals- milk (12/22). All the strains were confirmed by PCR targeting amplification of the femA gene. Out of 52 PCR-confirmed S. aureus samples, 17 were enterotoxin-producing strains. The …
The Relationship Of Ultrasound Measurements Taken From Two Different Anatomical Regions To Carcass Traits And Chemical Composition Of The Carcass In Karayaka Lambs, Fi̇li̇z Akdağ, Bülent Teke, Mustafa Uğurlu, Mustafa Salman, Yücel Meral
Turkish Journal of Veterinary & Animal Sciences
This study was undertaken to determine the relationship between carcass traits and chemical compositions. Using ultrasound scanning from two different anatomic regions, the study involved estimating carcass traits and chemical composition in male Karayaka lambs. Measurements were taken of skin thickness, subcutaneous fat thickness, muscle depth, muscle width, and muscle area between the 12th and 13th thoracic vertebrae (12- 3T) and 3rd and 4th lumbar vertebrae (3-4L) using real-time ultrasound in 15 six-month-old lambs. The lambs were slaughtered after the ultrasound measurements, and then their warm and cold carcass weights; dressing percentage; bone, meat, and fat amounts; and chemical composition …
Effect Of Dosage And Duration Of Altrenogest Treatmenton Follicular Development And Ovulation In Sows*,
2017
TÜBİTAK
Effect Of Dosage And Duration Of Altrenogest Treatmenton Follicular Development And Ovulation In Sows*, Supatee Kitkha, Alongkot Boonsoongnern, Nattavut Ratanavanichrojn, Pichai Jirawattanapong, Anuchai Pinyopummin
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the effect of altrenogest (ALT) treatment on follicular development and ovulation in sows. Sows (parity 6-9; D0: weaning day) were assigned to four treatments as follows: control (no ALT treatment; n = 20), ALT-4-0 (ALT 20 mg/day, 5 days; D-4-D0; n = 20), ALT-4-2 (ALT 20 mg/day, 7 days; D-4-D2; n = 23), and 2ALT-4-2 (ALT 20 mg twice a day [40 mg/day], 7 days; D-4-D2; n = 24). The sows in each group were further divided into three groups based on slaughter periods: day 1 (DF1) and day 4 (DF4) of …
Maternal Behaviour Of Awassi Sheep And Behaviour Of Thelambs During The First Hour After Parturition,
2017
TÜBİTAK
Maternal Behaviour Of Awassi Sheep And Behaviour Of Thelambs During The First Hour After Parturition, Sabri̇ Gül, Özkan Görgülü, Mahmut Keski̇n, Zühal Gündüz
Turkish Journal of Veterinary & Animal Sciences
Data from sixty-one 2-5-year-old Awassi ewes (38 single and 23 twin births) were evaluated in order to determine behaviour at parturition and the following first hour. Observations were subjected to Kolmogorov-Smirnov and Shapiro-Wilk tests, Pearson-s chi-square test, independent samples t-test, and ANOVA. The Awassi sheep exhibited sniffing action after parturition as the initial behaviour, choosing the back part of the lamb. First licking and sniffing times were found to decrease with maternal age; calculated average data for these characteristics were 138.3 - 15.39 and 118.3 - 26.48 s for singlets, and 147.7 - 15.12 and 123.6 - 29.54 s for …
Antiinflammatory Effect Of Herbal Preparations On Adjuvant Arthritis In Rats,
2017
TÜBİTAK
Antiinflammatory Effect Of Herbal Preparations On Adjuvant Arthritis In Rats, Laimis Akramas, Laima Leonaviciene, Ruta Bradunaite, Audrius Vasiliauskas, Irena Dumalakiene, Danguole Zabulyte, Teresa Normantiene, Irena Jonauskiene, Dalia Vaitkiene
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to investigate the antiinflammatory effects of 2 herbal extracts (CDS and CDSM) in rats with experimental adjuvant arthritis (AA). Joint swelling, development of polyarthritis, blood indices, pro/antioxidant activity, and proinflammatory cytokine IL-17 in blood serum, as well as the liver and paw histopathology and histochemistry, were assessed. Oral injections of extracts showed an antiinflammatory effect analogous to that of diclofenac (DF), improved blood indices and suppressed level of IL-17, significantly diminished joint swelling, and histological changes in the joints. Increase in CAT activity and AOA and reduction in lipid peroxidation was noted. The preparations did not …
Identification Of Candida Strains With Nested Pcr In Bovinemastitis And Determination Of Antifungal Susceptibilities,
2017
TÜBİTAK
Identification Of Candida Strains With Nested Pcr In Bovinemastitis And Determination Of Antifungal Susceptibilities, Göksel Erbaş, Uğur Parin, Şükrü Kirkan, Serap Savaşan, Volkan Özavci, Hafi̇ze Tuğba Yüksel
Turkish Journal of Veterinary & Animal Sciences
Mastitis is one of the biggest problems of dairy cattle industries. In recent years, fungal agents have been frequently reported among the causative agents for mastitis. Among the fungal agents, Candida species are the most common. This study's aims were the isolation, identification, and determination of antifungal susceptibility of Candida species causing mycotic mastitis in cattle. A total of 260 mastitic milk samples were collected from different farms. Identification was conducted by rapid diagnostic tests and nested PCR method; 6 different antifungal agents were examined. Candida sp. was detected in 46 (17.7%) of the 260 mastitic milk samples. Based on …
Barley As An Alternative To Rice In Dog Food,
2017
TÜBİTAK
Barley As An Alternative To Rice In Dog Food, Fatma İnal, Mustafa Selçuk Alataş, Oğuzhan Kahraman, Şeref İnal, Mustafa Uludağ, Emel Gürbüz, Esad Sami̇ Polat
Turkish Journal of Veterinary & Animal Sciences
In this study, 30 mixed-breed, neutered, young adult male dogs at 15-30 kg live weights were used. The foods were produced at a special facility. Dog food produced using 25% barley had a gelatinized starch content significantly greater than that of food produced using 25% rice: 18.36% vs. 17.45%, respectively. No considerable difference was observed in the dogs' preference between the two foods (50.95% vs. 49.05%). Dry matter digestibility of rice and barley food was 86.10% and 83.85% and crude protein digestibility was 80.44% and 79.24%, respectively. Based on the consumption of rice or barley, stool consistency did not change.
Effect Of Varying Levels Of Concentrate On Growth Performance And Feed Economics In Nili-Ravi Buffalo Heifer Calves,
2017
TÜBİTAK
Effect Of Varying Levels Of Concentrate On Growth Performance And Feed Economics In Nili-Ravi Buffalo Heifer Calves, Zeeshan Muhammad Iqbal, Muhammad Abdullah, Khalid Javed, Makhdoom Abdul Jabbar, Nisar Ahmad, Yasir Allah Ditta, Hamid Mustafa, Faisal Shahzad
Turkish Journal of Veterinary & Animal Sciences
An experiment was conducted to optimize the effect of concentrate levels on growth performance in Nili-Ravi buffalo heifers. These animals (n = 30), aged 4-6 months, were divided into three treatment groups (A, B, C) and were offered concentrate at the rate of 0.5%, 1.0%, and 1.5% of body weight for an 8-month period, respectively. The statistical data showed a significant (P < 0.01) effect on dry matter intake among all treatment groups. However, dietary treatments showed a nonsignificant effect on body weight gain. Treatment A showed a better (P < 0.01) feed conversion ratio (10.01 - 0.55) compared to treatment B (11.75 - 0.79) and C (12.85 - 0.74). The average body condition score, body measurements, and blood profiles were not affected by varying levels of concentrate. However, total serum protein was found lower (P < 0.01) in treatment A compared with the other two treatment groups. The cost for production per kg gain was 80.8% higher in treatment C and 46.6% higher in treatment B compared to treatment A. It can be concluded that buffalo heifers can be reared cost-effectively on a minimum concentrate level (0.5% of body weight) along with ad libitum green fodder.
Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog,
2017
TÜBİTAK
Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur
Turkish Journal of Veterinary & Animal Sciences
A 10-year-old spayed female Maltese dog presented to a local animal hospital with a subcutaneous mass (4 x 3 x 3 cm) in the right shoulder region. The mass was well circumscribed, with soft tissue opacity and variable levels of mineralization, but with no bony involvement in radiography. The mass was surgically removed. Upon histological examination, the mass consisted of malignant mesenchymal cells that had produced a chondroid matrix and osteoid. Round to polygonal neoplastic cells with large nuclei showed moderate anisokaryosis and variable numbers of mitotic figures. The tumor cells were positive for vimentin and osteocalcin and negative for …
Association Of Porcine Cryptorchidism With Dna Parentage Test, Testicular Protein Expression And Testicular Proteomics,
2017
Faculty of Veterinary Science
Association Of Porcine Cryptorchidism With Dna Parentage Test, Testicular Protein Expression And Testicular Proteomics, Nathamon Yimpring
Chulalongkorn University Theses and Dissertations (Chula ETD)
Cryptorchidism is one of the most common congenital abnormalities observed in boars. One of the important obstacles to produce good quality pork is cryptorchidism. Pigs with cryptorchidism cause defects in spermatogenesis, low conception rate and/or infertility. This odor can be the cause of economic loss because consumers reject to buy meat with boar taint and flavor. In fattening farm, multi-sire mating system is normally used. Molecular genetic techniques allow the possibility of testing on genetic and parentage control. This research aimed to study i. To optimize microsatellite markers and develop the DNA fingerprint technique used to identify the parentage of …
