Veterinary Medicine Commons^™

Semi-Automated Microbiopsy Device: A Potential Tool For Muscle Sampling In Horse, Metha Chanda, Ratchakrit Srikuea, Pawinee Piyachaturawat

The Thai Journal of Veterinary Medicine

Muscle biopsy is considered as a gold standard for evaluations of muscle integrity and pathological conditions in horse skeletal muscle. A semi-automated microbiopsy device with small needle has recently been introduced in horse muscle research, however there is no report on the quality of the muscle tissue obtained by the device. The aim of the present study was to examine the potential of the semi-automated microbiopsy device for horse skeletal muscle tissue sampling by evaluating the quality of samples for histological and biochemical analyses. Gluteus medius muscle samples were taken from six mature polo ponies and three ageing polo ponies …

Optimized Codons Of Polar Tube Protein 1 Gene Of Encephalitozoon Cuniculi To Enhance Protein Expression In Escherichia Coli, Waraporn Sinsuwonkwat, Pornchai Sanyathitiseree, Sakuna Phattanakunanan, Siriluk Jala, Preeda Lertwatcharasarakul

The Thai Journal of Veterinary Medicine

Encephalitozoon cuniculi (E. cuniculi) is an intracellular zoonotic parasite that is recognized as a common parasite of rabbits. Infected rabbits show neurological and renal disorder. The polar tube protein 1 of the spore is revealed to be antigenicity. The present study aimed to produce a recombinant polar tube protein 1 (rPTP1) for which the codon usage of a native ptp1 gene was optimized and synthesized for protein production in Escherichia coli (E. coli). Both a native ptp1 gene amplified by PCR technique and an optimized ptp1 gene were ligated into a pQE30 expression vector and transformed into an E. coli …

Modulatory Effects Of Pg102 (Actinidia Arguta) On Inflammatory Cytokine Mrna Expression In Canine Atopic Dermatitis, Yong-Uk Lee, Seol-Hee Park, Eui-Hwa Nam, Tae-Ho Chung, Cheol-Yong Hwang

The Thai Journal of Veterinary Medicine

Canine atopic dermatitis (AD) is a common, chronic relapsing inflammatory skin disease. An aqueous extract, PG102, derived from Actinidia arguta fruit, has been investigated as an anti-inflammatory agent in humans and atopy-like animal models, but its underlying mechanism for canine AD treatment has not been fully understood. This study evaluated the in vitro anti-inflammatory effect of PG102 with respect to the representative allergy-related cytokine panel from dust mite allergen extract (DME)-stimulated peripheral blood mononuclear cells (PBMCs) of canine AD patients using quantitative real-time PCR. PBMCs were isolated from eight dogs with definite diagnoses of AD and stimulated with DME for …

Osteogenic Differentiation Potential Of Canine Bone Marrow-Derived Mesenchymal Stem Cells Under Different Β-Glycerophosphate Concentrations In Vitro, Chenphop Sawangmake, Sirirat Nantavisai, Thanaphum Osathanon, Prasit Pavasant

The Thai Journal of Veterinary Medicine

Stem cell-based therapy employing bone marrow-derived mesenchymal stem cells has been proposed as a promising approach for bone regenerative treatment. Despite numerous studies supporting the application in human medicine, such information in veterinary practice is still lacking. Thus, this study aimed to investigate in vitro osteogenic differentiation potential of canine bone marrow-derived mesenchymal stem cells under different doses of β-glycerophosphate supplement. The isolated cells showed self-renewing ability and expressed pluripotent marker genes, zinc finger protein 42 and octamer-binding transcription factor 4, suggesting their potentiality in vitro. In vitro osteogenic differentiation was performed by using a regular osteogenic induction medium containing …

Inducible Vancomycin Resistance Is Common In Porcine Enterococcus Gallinarum And E. Casseliflavus Isolates, Wanida Tharvornvee, Chantima Pruksakorn, Porntippa Lekcharoensuk

The Thai Journal of Veterinary Medicine

Enterococcus gallinarum and E. casseliflavus possess intrinsic vancomycin resistance, which is mediated by vanC-1 and vanC-2 gene clusters, respectively. The VanC resistance phenotype is expressed inducibly or constitutively. This study characterized the expression types for vancomycin resistance in 23 porcine E. gallinarum and 19 porcine E. casseliflavus isolates with vancomycin minimum inhibitory concentrations between 4 and 16 μg/ml. The vanSC and vanSC-2 genes were sequenced and the deduced amino acid sequences were compared to those of the inducible and constitutive resistance reference strains. Analysis of growth curves revealed that the resistance phenotype in all isolates was induced by vancomycin. In …

Renoprotective Effects Of Megadose Vitamin C On Cisplatin-Induced Kidney Injury, Supa Sithanukul, Chollada Buranakarl, Chutamas Benjanirut, Anudep Rungsipipat

The Thai Journal of Veterinary Medicine

Cisplatin (CDDP)-induced nephrotoxicity is mediated via oxidative stress and may be alleviated using antioxidant activity. Although megadose of vitamin C may cause oxalate nephropathy and apoptosis, the antioxidant effect of vitamin C may be beneficial when given along with CDDP. The objective of this study was to investigate the renoprotective effects of megadose vitamin C on rats receiving CDDP. Rats were divided into 4 groups: group 1, control rats (CONT); group 2, vitamin C-treated rats (VIT C); group 3, CDDP-treated rats (CDDP) and group 4, CDDP + vitamin C-treated rats (CDDP + VIT C). Vitamin C (1000 mg/kg) was given …

Dietary Supplementation Of Condensed Tannin Decreased Lipid Peroxidation In Muscle Of Nile Tilapias (Oreochromis Niloticus), Natthana Thitichayaphong, Theera Rukkwamsuk

The Thai Journal of Veterinary Medicine

The effect of condensed tannin supplementation in diet on lipid peroxidation in the muscle was evaluated in Nile tilapias (Oreochromis niloticus). In total, 250 fish, aged 21 days old, were randomly allocated into 10 experimental tanks, 25 fish per tank. Five tanks were set as the control group and 5 tanks as the supplemented group. The control fish were fed a commercial diet, while the supplemented fish were fed the same control diet plus 4 g/kg of condensed tannin. The diets were offered at a rate of 8% of estimated fish body weight twice daily. The experimental period lasted for …

Effects Of Swimming Frequency On Body Weight And Serum Lipid Profile In Small-Breed Dogs During A Four-Month Period, Korakot Nganvongpanit, Napat Ruamrungsri, Boonyapawn Tepsoontorn, Terdsak Yano, Puntita Siengdee, Siriphun Kongsawasdi

The Thai Journal of Veterinary Medicine

Lack of activities for dogs, that is, decreased body motion and lack of opportunities to exercise, can cause dogs to become overweight. Swimming is one of the best physical exercises for a dog that has been approved by a veterinary doctor, and it provides a form of supported exercise. Almost all the muscles are required for the movement that involves improving strength, working the cardiovascular and respiratory system, and increasing the metabolic rate withoutimpact on joints. One interesting aspect of swimming is its ability for weight and serum lipid control. The effect of frequency-controlled swimming activity on a dog’s weight …

Prevalence Of Bartonella Henselae, Bartonella Clarridgeiae, And Bartonella Vinsonii Subsp. Berkhoffii In Pet Cats From Four Provincial Communities In Thailand, Wilasinee Srisanyong, Ratree Takhampunya, Thidarut Boonmars, Anusak Kerdsin, Fanan Suksawat

The Thai Journal of Veterinary Medicine

Bartonella species are Gram-negative alpha proteobacteria and intracellular parasites of erythrocytes, endothelial and dendritic cells. Many are zoonotic pathogens of various mammalian reservoir hosts and are transmitted by blood-feeding arthropods. Recently, there have been several reports indicating that cats are important reservoir hosts for Bartonella pathogens, including those that cause cat scratch disease affecting humans in many countries including in Thailand. To assess more completely the role of cats as reservoir hosts for Bartonella pathogens in Thailand, 139 blood samples were collected from companion cats from a single community in each of four provinces (Khon Kaen, Kalasin, Nakhon Si Thammarat …

Amyloidosis Associated With Feline Leukemia Virus In A White Bengal Tiger (Panthera Tigris Tigris), N.I. De La Cruz-Hernández, J.O. Merino-Charres, E.M. Salinas-Navarrete, A.E. Monreal Garcia, J. Martinez Burnes, J.A. Rangel Lucio, C. Venegas-Barrera

The Thai Journal of Veterinary Medicine

Feline leukemia virus (FeLV) is the cause of neoplastic and degenerative disorders and immunosuppression in domestic cats. FeLV has never been reported in Bengal tiger (Panthera tigris tigris). Necropsy findings of a white Bengal tigress were a subcutaneous mass of 10 cm in diameter in the right rear limb which was classified as fibrosarcoma. In agreement with macroscopic and microscopic lesions, the morphologic diagnosis was consistent with a multisystemic disease characterized by fibrinonecrotic and suppurative broncopneumonia with multifocal severe bronchiectasis, severe icteric hepatomegaly and renal amyloidosis with membranoproliferative glomerulonephritis. Furthermore, a serum sample was positive to FeLV when diagnosed by …

Efficacy Of 10% W/V Fipronil Spot-On Against Tick (Rhipicephalus Sanguineus) Infestation On Cats, Sonthaya Tiawsirisup, Wilai Rattanatayarom

The Thai Journal of Veterinary Medicine

The efficacy of 10% w/v fipronil spot-on for treatment and prevention of Rhipicephalus sanguineus infestation on cats was examined. Twelve cats were randomly allocated into two groups which were the treatment and control groups. The treatment group received 10% fipronil spot-on at a dosage of 6.7 mg/kg on day 0. A group of 60 R. sanguineus was released to feed on each cat on days -7, -2, 3, 7, 14, 21, and 28. The ticks were counted and removed on days -4, 3, 6, 10, 17, 24, and 31. The infested ticks on the cats were also counted but not …

Prevalence Of Aada1, Aada2, Aadb, Stra And Strb Genes And Their Associations With Multidrug Resistance Phenotype In Salmonella Typhimurium Isolated From Poultry Carcasses, Abbas Doosti, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Mokhtari-Farsani

The Thai Journal of Veterinary Medicine

This study aimed to assess the prevalence of aminoglycoside resistance genes in S. Typhimurium isolated from poultry carcasses in Iran, and to reveal the most prevalent patterns of antimicrobial resistance. A total number of 300 samples of poultry carcasses were analyzed. Salmonella was isolated from 245 samples (81.66%). Multiplex PCR showed that 56.3% of the samples belonged to serovar S. Typhimurium and the remainder (43.6%) contained the rest of serovars. The highest rate of drug resistance was observed for tetracycline (97.0%), nalidixic acid (87.0%) and amoxicillinclavulanic acid (67.4%). These serovars, however, were sensitive to cefotaxime (84.8%), sulfamethoxazole trimethoprim (77.6%) and …

Chikungunya Virus Infection In Balb/C And Icr Mouse Models, Sonthaya Tiawsirisup, Ranida Tuanudom, Nichapat Yurayart

The Thai Journal of Veterinary Medicine

Chikungunya virus (CHIKV) infection is an emerging or re-emerging infectious disease found in several countries. The ecology of this virus involves humans, reservoir animals, and mosquito vectors. Two experiments were conducted in this study. Blood samples were collected and tested for CHIKV infection by using immunocytochemistry (ICC) staining and reverse transcription polymerase chain reaction (RT-PCR). For the first experiment, BALB/c mice were inoculated with 106.3 CID50/mouse. Mean CHIKV titers in the BALB/c mice were 103.3, 101.5, 102.3, and 102.3 CID50/ml of serum on day 1 to day 4 post inoculation (PI), respectively. For the second experiment, there were two groups …

Balloon Valvuloplasty For Pulmonic Stenosis In Two Dogs: Case Report, Chollada Buranakarl, Anusak Kijtawornrat, Wasan Udayachalerm, Sirilak Disatian Surachetpong, Saikaew Sutayatram, Pasakorn Briksawan, Sumit Durongpongtorn, Rampaipat Tungjitpeanpong, Nardtiwa Chaivoravitsakul

The Thai Journal of Veterinary Medicine

Balloon valvuloplasty for correction of congenital pulmonic stenosis (PS) in two dogs by intervention technique was first reported in Thailand. Both dogs did not show any clinical signs related to congestive heart failure. Physical examination in both dogs revealed heart murmur. Electrocardiographs of both dogs were recorded and showed normal sinus rhythm with deep S wave. In the first dog, thoracic radiograph revealed cardiomegaly with enlargement of the main pulmonary artery and left deviation of the apex due to right ventricular enlargement. Definitive diagnosis of PS was performed by echocardiography. Computed tomography (CT) of both dogs demonstrated stenosis of the …

Immunophenotyping Of Cutaneous Extramedullary Plasmacytoma In Two Dogs, Chutchai Piewbang, Sirintra Sirivisoot, Sitthichok Lacharoje, Anudep Rungsipipat, Somporn Techangamsuwan

The Thai Journal of Veterinary Medicine

Skin biopsied tissues from two dogs, initially diagnosed as hemophagocytic histiocytic sarcoma (HHS) by histopathology, were immunostained with a series of antibodies against lambda light chains, kappa light chains, multiple myeloma oncoprotein 1 (MUM-1), CD-3, paired box 5 (PAX-5), E-cadherin and lysozyme. Heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) was additionally performed on formalin-fixed paraffin-embedded (FFPE) tissues to rule out lymphomas from histiocytomas. Results showed that both cases were immunopositive for lambda light chains, MUM-1, PAK-5 and lysozyme, indicating a diagnosis of extramedullary plasmacytoma (EMP). Moreover, negative PCR result provided additional evidence confirming the diagnosis. Even though hPARR …

Ecg Quiz, Chollada Buranakarl

The Thai Journal of Veterinary Medicine

No abstract provided.

Ophthalmology Snapshot, Nalinee Tuntivanich

The Thai Journal of Veterinary Medicine

No abstract provided.

What Is Your Diagnosis?, Chutimon Thanaboonnipat, Nan Choisunirachon

The Thai Journal of Veterinary Medicine

No abstract provided.

Morphological And Biochemical Adaptive Changes Associated With A Short-Period Starvation Of Adult Male Japanese Quail (Coturnix Japonica), Yasser Ahmed, Soha A. Soliman, Mohammed Abdelsabour-Khalaf

Histology Collection

Objective: The morphological and biochemical impact of a short-period of starvation on Japanese quail was investigated.

Materials and Methods: Ten adult male Japanese quail were divided into two groups; control fed and starved. The control-fed group was offered food and water ad libitum and the starved group was subjected to a short-period of food deprivation. After 2.5 days, the serum was obtained and different parameters including the total protein, AST, ALT, triglyceride, HDL, LDL, creatinine and urea were assessed. Gastrointestinal tract, stomach and liver were excised and their masses were estimated. Paraffin and resin embedded sections from the proventriculus, gizzard, …

Methamphetamine: A Substance Of Emerging Environmental Concern For Horse Racing, Clara Fenger, Tanya Boulmetis, Kim Brewer, Kent Stirling, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

No abstract provided.