Veterinary Medicine Commons^™

Comparison Of Different Paternity Test Panels In Sheep, Onur Yilmaz, İbrahi̇m Cemal, Behi̇ç Coşkun, Yusuf Zi̇ya Oğrak, Nezi̇h Ata, Orhan Karaca

Turkish Journal of Veterinary & Animal Sciences

The aims of this study were to evaluate different paternity test panels with 17 microsatellite markers for their effectiveness in paternity analysis and to identify pedigree error rates in the Kangal Akkaraman breed of sheep. The animal material for the study consisted of 175 Kangal Akkaraman sheep, 35 rams, and their 140 offspring, raised on farms and bred using controlled mating. The panels were created according to probability of exclusion (PE) of 17 studied microsatellites. A total of 240 alleles were detected across 17 microsatellite loci. The overall mean value of the polymorphic information content (PIC) (0.78) indicated that the …

The First-Time Isolation Of Brucella Canis From Two Aborted Bitches In A Kennel In Turkey, Ahmet Murat Sayteki̇n, Mustafa Sencer Karagül, Buket Altuntaş, Gülseren Yildiz Öz, Gülnaz Meci̇toğlu, Özge Yilmaz

Turkish Journal of Veterinary & Animal Sciences

Brucella canis is defined as the specific agent that is responsible for canine brucellosis. In this study, B. canis was isolated and identified from the clinical materials belonging to 2 dogs kept in a breeding kennel in Turkey and brought to the researchers? clinic with abortion complaints. With this study, for the first time ever, B. canis isolation and identification was made from dogs that aborted in a kennel. This study emphasizes

Survey Of Dirofilaria Immitis In Dogs From Sivas Province In Thecentral Anatolia Region Of Turkey, Ahmet Duran Ataş, Kürşat Altay, Ahmet Ali̇m, Erkan Özkan

Turkish Journal of Veterinary & Animal Sciences

In this research, we investigate the prevalence of Dirofilaria immitis infection in dogs using microscopical, serological, and molecular methods from the Central Anatolia Region (Sivas) of Turkey and reveal the associated risk factors. Whole blood and serum samples were obtained from 306 dogs older than 7 months. Polymerase chain reaction (PCR) and the modified Knott's method (Knott) were used for detecting the microfilaria and enzyme-linked immunosorbent assay (ELISA) was applied for the presence of D. immitis antigens. The prevalence of D. immitis in dogs was detected as 2.9% (9/306) by PCR and ELISA and as 1.3% (4/306) by Knott. All …

Application Of Reproductive Biotechnologies For Sustainableproduction Of Livestock In Turkey, Abdullah Kaya, Erdoğan Güneş, Erdoğan Memi̇li̇

Turkish Journal of Veterinary & Animal Sciences

Reproductive biotechnology encompasses powerful tools to enhance the efficiency and profitability of livestock reproduction, production, and product quality. These technologies include genomic selection, evaluation of semen (from bulls, bucks, and rams) using advanced cell and molecular technologies, semen cryopreservation and artificial insemination, estrus synchronization, superovulation of the females (cows, dams, and ewes), ovum pick-up, in vitro fertilization and embryo culture, embryo transfer, and pregnancy detection. The extent of applications of these modern technologies and their economic impacts for livestock production in Turkey are elusive. As a result, there is an urgent need for sound economic analyses, research and education, training …

Evaluation Of Real-Time Pcr, Mat, And Recombinant Lipl32-Based Elisa For The Diagnosis Of Canine Leptospirosis In A Disease-Endemic South Indian State, Kerala, Siju Joseph, Mangattumuruppel Mini, Vamshi Krishna Sriram, Ramachandran Ambily, Thazhathuveettil Aravindakshan, Sreedharannair Ajithkumar

Turkish Journal of Veterinary & Animal Sciences

Canine samples (n = 425) collected from cases of suspected leptospirosis over a period of 6 years were tested using the microscopic agglutination test (MAT), recombinant LipL32-based IgM and IgG ELISA, and real-time PCR (RT PCR). A prevalence of 69.41% was recorded by MAT. Among the different tests, IgG ELISA was observed to have maximum agreement with that of MAT (k-0.810), followed by IgM ELISA (k-0.762.). RT PCR had only slight agreement with MAT (k-0.156). However, the percentage positivity of RT PCR with samples with MAT titers of 1:100 and 1:200 were 76.47% and 85.96%, respectively, indicating the reliability of …

Determination Of In Situ Degradation Kinetics Of Some Legume Waste Not Used For Human Consumption, Şevket Evci̇, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

The objective of this experiment was to evaluate the chemical composition, in situ ruminal degradation kinetics, and protein fractions of subsieve dry beans (DB), chickpeas (CCP), red lentils (RL), and green lentils (GL). Four samples of those legumes were utilized as replicates and were incubated for up to 48 h in the rumen of 3 rams. RL and CCP had higher organic matter (OM) than DB and GL. Crude protein (CP) was the highest in GL (P < 0.05). Ether extract (EE) concentrations were higher in CCP and DB compared to those of RL and GL (P < 0.05). Crude fiber (CF), acid detergent fiber (ADF), and neutral detergent fiber (NDF) contents were the highest in RL (P < 0.05). The acid insoluble nitrogen (ADIN-N) content was similar in all samples evaluated. While RL had the lowest OM and CP degradability and water-soluble OM and CP concentrations, DB and CCP had the highest potentially degradable OM and CP contents at the end of the 48 h incubation period (P < 0.05). Escaped protein content was the highest in RL and the lowest in DB (P < 0.05). In conclusion, some of the pulse species that are produced locally and not suitable for human consumption can be used in the diet of ruminant animals as a source of protein based on OM and CP degradabilities and escape protein contents.

Complete Genome Sequence Of Moraxella Bovis Strain Epp-63 (300), An Etiologic Agent Of Infectious Bovine Keratoconjunctivitis, John Dustin Loy, Aaron M. Dickey, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

We report here the complete closed genome sequence of Moraxella bovis strain Epp-63 (300) (Epp63). This strain was isolated from an infectious bovine keratoconjunctivitis (IBK) case in 1963. Since then, Epp63 has been used extensively for IBK research. Consequently, the genome sequence of Epp63 should help elucidate IBK host-pathogen interactions.

The Effect Of Green Tea Waste Extract On Ruminal Degradability And Intestinal Digestibility Of Barley Grain, Mohammad Nasehi, Nor Mohammad Torbatinejad, Morteza Rezaie, Taghi Ghoorchi

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to investigate the effects of different levels of green tea waste extract (GE) on ruminal degradability and intestinal digestibility of dry matter (DM), crude protein (CP), and starch of barley grain. Therefore, barley grain was treated with 0 as control, and 5%, 10%, 15%, and 20% of GE. The potential of DM degradability (a + b) of barley grain treated with 15% and 20% of GE was significantly lower compared to other treatments (Linear [L] P < 0.05). The lowest (a + b) fraction of CP degradability was found in the barley grain treated with 20% GE (L P < 0.05). The (a + b) fraction and effective degradability of starch in barley grain treated with 15% and 20% of GE were significantly lower than those in other treatments (L and quadratic [Q] P < 0.05). The highest intestinal DM, CP, and starch digestibility were associated with barley grain treated with 10% GE (L and Q P < 0.05). Based on recent results, processing barley grain with GE leads to a decrease of DM, CP, and starch degradability. The treatment of 10% GE improved intestinal digestibility of the aforementioned nutrients; therefore, it can be used to protect barley grain against ruminal degradability.

Comparative Analysis Of Low Molecular Weight Fraction Of Conditioned Media Derived From Starvation-Dormant And Anaerobically-Dormant Mycobacterium Avium Subsp. Paratuberculosis, Marina Buciuc

All Graduate Theses, Dissertations, and Other Capstone Projects

Johne's disease (JD) caused by Mycobacterium avium subsp. paratuberculosis (MAP) is a contagious chronic enteritis affecting all ruminants and causing economic loss of at least $250 million annually in the US dairy industry. One of the major limitations in the diagnosis of JD is the low sensitivity of fecal culture in animals with no clinical signs of the disease, but who are actively shedding MAP. Persistence of MAP in the host with no apparent signs of infection is attributed to its ability to enter a dormant state wherein the bacterium is viable, but is not able to replicate until conditions …

Rhodococcus Equi In The Foal – Improving Diagnostic And Prevention Measures, Fernanda Bicudo Cesar

Theses and Dissertations--Veterinary Science

Although Rhodococcus equi (R. equi), previously known as Corynebacterium equi, was first isolated from pneumonic foals almost a century ago, it remains the most common cause of subacute or chronic granulomatous bronchopneumonia in foals. While the majority of foals exposed to R. equi develop a protective immune response (regressors), others exhibit a unique susceptibility to infection (progressors). The determinants for either outcome are not completely understood. Therefore, current diagnostic and preventive measures are suboptimal and require betterment. In light of this current need, we hypothesized that immunoglobulin G subisotype T [IgG(T)] against the virulence-associated protein A (VapA) …

Cellular And Molecular Basis Of Equine Arteritis Virus Persistent Infection In The Stallion Reproductive Tract: Characterization Of Local Host-Pathogen Interactions Mediating Long-Term Viral Persistence, Mariano Carossino

Theses and Dissertations--Veterinary Science

Equine arteritis virus (EAV) has a global impact on the equine industry being the causative agent of equine viral arteritis (EVA), a reproductive, respiratory, and systemic disease of equids. A distinctive feature of EAV infection is that it establishes long-term persistent infection in the reproductive tract of stallions and is continuously shed in the semen (carrier state). Recent studies showed that long-term persistence is associated with a specific allele of the CXCL16 gene (CXCL16S). However, the cellular and molecular mechanisms underlying the establishment and maintenance of persistent infection are yet to be determined. The studies were undertaken herein …

2018 Nebraska Beef Cattle Report (Complete)

Nebraska Beef Cattle Reports

Contents

Cow/Calf: Analyses of Birthdate and Growth in Beef Heifers Categorized by Puberty and Pregnancy Status • Letrozole: A Steroid-Free Estrous Synchronization Method • Comparison of Two Alternate Prostaglandin Products in Yearling Beef Heifers • Impact of Pre-and Postpartum Nutrition on March-calving Cow and Progeny Productivity • Effects of Late Gestation Supplementation, Synchronization, and Creep Feeding in a Spring Calving Beef Herd in the Nebraska Sandhills • Effect of Supplementation during the Breeding Season on a May-calving Herd in the Nebraska Sandhills • Impact of Winter Supplementation of May Calving Cows and Heifer Development System in Two Different Breeding Seasons …

Impact Of Intake On Methane Production In Growing Steers, Tom M. Winders, Bradley M. Boyd, F. Henry Hilscher Hilscher, Samodha C. Fernando, Richard Stowell, Galen E. Erickson

Nebraska Beef Cattle Reports

A study was conducted to evaluate the impact that level of intake has on methane and carbon dioxide production by growing steers. Two treatments were evaluated that included ad-libitum intake compared to limit-fed steers. The ad-libitum fed cattle had greater gains, similar feed efficiency and produced more methane and carbon dioxide per day, while the limit fed cattle produced more methane and carbon dioxide per pound of intake than the ad-libitum fed cattle.

Supplementing Rumen Undegradable Protein To Grazing Cattle, Braden C. Troyer, Bradley M. Boyd, Andrea K. Watson Watson, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

A pooled-analysis of previous Nebraska Beef Report Articles examined the impact of rumen undegradable protein (RUP) supplementation for cattle grazing different types of forage. Each lb of RUP supplement increased ADG by 0.63 lb/d when cattle were grazing smooth brome and 0.43 lb/d when grazing warm season grasses. Cattle did not respond to RUP when grazing summer annuals which were high (18.2%) in CP.

Experience Improves The Reliability Of Subjective Measurements Of Temperament In Beef Cattle, Jaime T. Praham, Amy E. Tanner, Mark L. Wahlberg Wahlberg, W. S. Swecker Jr., Ronald M. Lewis

Nebraska Beef Cattle Reports

Reliability of experienced and inexperienced observers when assessing the behavior of cattle when restrained in a squeeze chute (chute score), and when exiting the chute (exit score), was compared. Overall, experienced observers had higher reliability than inexperienced observers. Increasing the number of individuals scoring an animal decreased the degree of agreement. However, within an acceptable tolerance for difference in scores, such disagreement may be beneficial; it allows for subtlety in interpretations of temperament, which when averaged, may better reflect docility. Reliabilities were higher for exit score than chute score. This may reflect the complexity of the trait being evaluated, with …

Antimicrobial Interventions And Application Time Effects On Ground Beef Quality, Ashley R. Mccoy, Dennis E. Burson, Gary A. Sullivan Sullivan

Nebraska Beef Cattle Reports

Small business meat processors can use organic acid antimicrobial interventions to control Shiga toxin-producing E. coli (STEC) when producing ground beef; however, many small producers are concerned about the impact on ground beef quality. The effects of two commonly used organic acids, lactic acid and peroxyacetic acid, were evaluated at short (15 seconds) or extended (3 minutes) raw material dip times on ground beef quality parameters. Beef trim dipped in lactic acid for 3 minutes had a reduction in total aerobic bacteria plate count, but also increased ground beef discoloration and lipid oxidation during retail display. Use of a shorter …

Steer Performance Grazing Corn Residue And Supplemented With Modified Distillers Grains Plus Solubles With Or Without Urea, Robert M. Jones, Robby G. Bondurant, F. Henry Hilscher Hilscher, Jim C. Macdonald

Nebraska Beef Cattle Reports

A growing study was conducted to evaluate the effects of supplementing growing calves grazing corn residue with modified distillers grains plus solubles (MDGS; 3 or 5 lb/d) and with or without urea on growth performance. There were no significant MDGS × urea inclusion interactions observed. Urea inclusion level (0 and 0.12 lb/d) did not affect supplemental intake, ADG, or ending BW. Steers fed 5 lb of MDGS had an increased ADG and a heavier ending BW compared to steers fed 3 lb MDGS daily. Supplemental urea is not necessary when supplementing at least 3 lb MDGS to steers grazing corn …

Evaluation Of Corn Distillers Solubles On Growing Steer Performance, Benjamin H. Hansen, Robert G. Bondurant, F. Henry Hilscher Hilscher, Galen E. Erickson, Jim C. Macdonald

Nebraska Beef Cattle Reports

A growing study evaluated increasing inclusions of corn distillers solubles (CDS) at 10, 20, 30, and 40%, or increasing wet distillers grains plus solubles (WDGS) at 10, 20, 30, and 40% compared to a corn control. Corn was replaced by CDS or WDGS in each forage-based diet. Increasing CDS resulted in a quadratic increase for both DMI and F:G. Increasing WDGS linearly increased both DMI and ADG with no effect on F:G. The energy value of CDS is less than that of corn, whereas WDGS had an energy value similar to corn in growing diets with 50% brome hay. A …

Analyses Of Birthdate And Growth In Beef Heifers Categorized By Puberty And Pregnancy Status, Aline Gomes Da Silva, Andy Roberts, T. L. Meyers, Richard N. Funston

Nebraska Beef Cattle Reports

Heifer records were retrospectively evaluated to see if Julian birthdate, cycling status prior to breeding, and body weight collected from weaning through final pregnancy diagnosis differed when heifers were categorized by 5 different approaches: 1) pubertal status prior to estrous synchronization, 2) whether or not detected in estrus at AI, 3) heifers impregnated by AI vs all other heifers, 4) final pregnancy status, and 5) a 5-way classification accounting for AI and pregnancy status (AI pregnant, heifers subjected to AI that subsequently conceived to bull, heifers not AI that were impregnated by bull, heifers subjected to AI that were not …

The Effect Of Harvest Method And Ammoniation Of Corn Residue On Growing Calf Performance, Ashley C. Conway, Robert G. Bondurant, F. Henry Hilscher Hilscher, James C. Macdonald, Terry J. Klopfenstein, Mary Drewnoski

Nebraska Beef Cattle Reports

A growing trial was conducted to determine the effect of feeding baled corn residue harvested using three different methods, with and without ammoniation of the residue. Residue harvested with the New Holland Cornrower™ with two rows of chopped stem added to the windrow resulted in a 9% increase in ADG compared to conventional rake and bale harvest or turning off the combine spreader and baling tailing. Ammoniation of residue increased ADG 67% (increase of 1.1 lb/d) over non-ammoniated residue. Feed efficiency was not affected by harvest method, but ammoniation decreased F:G by 13% compared to cattle fed non-ammoniated residue. Although …