Large or Food Animal and Equine Medicine Commons^™

Dietary Impact On Antibiotic Resistance In Feedlot Manure, Maranatha Zelt, Amy Schmidt, Noelle Mware, Xu Li, Galen E. Erickson

Nebraska Beef Cattle Reports

There is a growing public concern regarding antibiotic resistance and the use of antibiotics, including in livestock management. Understanding the ecology of antibiotic resistance among microbes, identifying resistance gene reservoirs, and implementing antibiotic resistance mitigation practices in livestock production are critical to protecting animal and human health while meeting increasing food demands. This research is one of several studies seeking to assess risk for livestock- to- human transfer of antibiotic resistance and to identify mechanisms for reducing that risk where possible. This study evaluated the impact of forage concentration and supplemental essential oil in beef cattle finishing diets on antibiotic …

Pseudomonas Survive Thermal Processing And Grow During Vacuum Packaged Storage In An Emulsified Beef System, Samuel C. Watson, Rebecca A. Furbeck, Byron D. Chaves, Gary A. Sullivan

Nebraska Beef Cattle Reports

New research has suggested the ability of Pseudomonas, a common spoilage microorganism, to grow in cooked beef products stored under vacuum which challenges the traditional understanding of the role of Pseudomonas during cooked beef spoilage. Understanding the mechanisms of survival and growth of Pseudomonas in these products is crucial for improving shelf life. The objective of this experiment was to determine Pseudomonas survival in a thermally processed, emulsified cooked beef model system. After eight weeks of refrigerated storage, Pseudomonas was recovered from cooked emulsified beef, indicating the potential for Pseudomonas to survive thermal processing and cause spoilage in cooked vacuum …

Accelerated Dry Aging Under Anaerobic Conditions, Joseph A. Sonderman, Soon K. Lau, Felipe A. Ribeiro, David M. Velasco, Nicolas A. Bland, Nicolas J. Herrera, Morgan L. Henriott, Jeyamkondan Subbiah, Chris Calkins

Nebraska Beef Cattle Reports

The purpose of dry aging is to develop novel favors and other sensory characteristics different from wet aged meat. However, leaving meat exposed to air for an extended period of time can have negative effects on meat quality. As the meat is exposed to oxygen for an extended period of time, lipids are oxidized resulting in compounds that negatively affect favor. In this study, oxygen concentration was regulated along with time, temperature, humidity, and air flow. The purpose of oxygen regulation was to determine the effect of oxidation on the quality, specifically flavor preference, of dry aged meats. Sensory analysis …

Economic Analysis Of Increased Corn Silage Inclusion In Beef Finishing Cattle, Hannah C. Wilson, J. C. Macdonald, Andrea K. Watson, Terry Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

An economic analysis was conducted to assess the feasibility of feeding greater inclusions of corn silage in finishing diets. Cattle were fed two inclusions of corn silage (15 and 45% of diet dry matter) with or without tylosin. Cattle fed 15% corn silage with tylosin had the best feed conversion, 15 % corn silage without tylosin was intermediate, and both 45% corn silage with and without tylosin had the poorest feed conversion. Feeding corn silage at greater inclusions decreased ADG but increased final body weight when fed to an equal fatness (28 days longer). However, feeding corn silage at 45% …

Impact Of Days Fed On Holstein Bull And Steer Performance And Cutability Of Cattle Pen- Fed Organic Diets, Elizabeth A. Schumacher, Braden C. Troyer, Bradley M. Boyd, Levi Mcphillips, J. C. Macdonald, Andrea K. Watson, Terry Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Performance, carcass characteristics, and total meat yield of Holstein bulls and steers were compared in a simulated organic production system with the goal of producing ground beef. Holstein bulls (n = 120, initial BW = 487 lb) and steers (n = 120, initial BW = 471 lb) of the same age were blocked by BW and assigned randomly to be harvested at 308, 343, 378, and 413 days on feed. After harvest, all meat o" the carcass was considered trim and was collected and weighed to calculate total trim yield. Bulls gained faster and had greater live body weight, carcass …

Training Improves The Reliability Of Temperament Assessment In Cattle, Jamie T. Parham, Jessica J. Schmidt, Ronald M. Lewis

Nebraska Beef Cattle Reports

Accurate and precise measurement of docility in cattle is paramount when including temperament as a criterion for selection. The value of training individuals in assigning a docility score was evaluated by comparing the reliability of individual assessments of temperament in beef cattle before and after various instructional methods. Preceding training, participants’ assessment of cattle behavior, videoed while each heifer was restrained in a chute, was not impacted by age, gender, or pre- existing cattle handling experience. Groups of participants that received additional training were more accurate and precise in evaluating temperament, regardless of training method, compared to those without. No …

Mineral Concentrations Of Forages For Livestock In Nebraska And South Dakota, Rebecca J. Kern, John W. Kern, Hannah M.G. Dorn, Carrie E. Putnam, Janna J. Block, Adele A. Harty, M. E. Drewnoski

Nebraska Beef Cattle Reports

Forage samples from Nebraska and South Dakota submitted to Ward Laboratories, Inc. from 2012– 2019 were analyzed for mineral concentrations. Samples were categorized by forage species, quality based on protein content, and mineral concentration based on requirements for lactating beef cows. ! e data indicate that copper and zinc are frequently deficient across all species and levels of forage quality, emphasizing the need for supplementation. Except for magnesium, macro- mineral deficiencies are less likely to occur when feeding high quality forages in Nebraska and South Dakota. Corn feedstuffs are particularly likely to result in mineral deficiencies if fed without mineral …

Evaluation Of Models Used To Predict Dry Matter Intake In Forage- Based Diets, Aksel Wiseman, Andrea K. Watson, Rick Stock, Terry Klopfenstein

Nebraska Beef Cattle Reports

Accurately predicting intake is critical to model performance of cattle in order to formulate diets to meet nutritional requirements. Modeling systems must be accurate in order to provide correct information to producers. Multiple studies with growing cattle consuming forage- based diets were summarized. Actual gain and weights of the cattle were used to determine predicted dry matter intake using the Beef Cattle Nutrient Requirements Model (2016). ! e predicted dry matter intakes were compared to observed dry matter intakes to determine accuracy of the prediction model. ! e model over predicted intakes at low TDN and under predicted intakes at …

Winter Growth Rate And Timing Of Marketing On Economics Of Yearling Systems, Michael Merical, M. E. Drewnoski, Jay Parsons

Nebraska Beef Cattle Reports

Economic analyses were conducted examining 18 years of Nebraska monthly- average auction data to ! nd the e" ects of certain management decisions on the pro! tability of yearling production systems. A 2×2 experimental design was used to examine four possible scenarios. $ e variables were either fast winter growth (daily gain, 2.0 lb/ day) or slow winter growth (daily gain, 0.8 lb/day), and either a September or a July marketing date. In addition to pro! tability, risk management was also examined in this study. Average pro! tability of all scenarios was good, ranging from $112 to $143 per calf. …

Impact Of Masters Choice Corn Silage On Nutrient Digestion In Growing Cattle, Jiehua Xiong, Mitch M. Norman, Hannah C. Wilson, Caleb Crabtree, Galen E. Erickson

Nebraska Beef Cattle Reports

A digestion study was conducted to evaluate Masters Choice corn silage hybrids on nutrient digestibility in growing beef steers. ! e three hybrids evaluated were a conventional hybrid (CON) commonly grown in Eastern Nebraska which served as the control, Masters Choice hybrid MCT6365 RIB (MC1) that has been selected to improve " ber and starch digestion and Masters Choice hybrid MCT6733 GT3000 (MC2) selected to improve " ber digestion. Treatment diets consisted of 80% of the diet dry matter (DM) of each corn silage hybrid. Steers fed MC1 corn silage had the greatest organic matter (OM), energy digestibility, and digestible …

Cellular Proliferation Of Equine Bone Marrow- And Adipose Tissue-Derived Mesenchymal Stem Cells Decline With Increasing Donor Age, Jasmin Bagge, James N. Macleod, Lise C. Berg

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: Bone marrow (BM)- and adipose tissue (AT)-derived mesenchymal stem cells (MSCs) are used increasingly for autologous cell therapy in equine practice to treat musculoskeletal and other injuries. Current recommendations often call for 10–100 million MSCs per treatment, necessitating the expansion of primary cells in culture prior to therapeutic use. Of concern, human and rodent studies have shown a decline of both MSC recovery from sampled tissue and in vitro proliferative capacity with increasing donor age. This may be problematic for applications of autologous cell-based therapies in the important equine demographic of older patients.

Objectives: To investigate the effect of …

Climate Change Is Likely To Increase The Development Rate Of Anthelmintic Resistance In Equine Cyathostomins In New Zealand, Christian W. Sauermann, Dave M. Leathwick, Mark Lieffering, Martin K. Nielsen

Maxwell H. Gluck Equine Research Center Faculty Publications

Climate change is likely to influence livestock production by increasing the prevalence of diseases, including parasites. The traditional practice of controlling nematodes in livestock by the application of anthelmintics is, however, increasingly compromised by the development of resistance to these drugs in parasite populations. This study used a previously developed simulation model of the entire equine cyathostomin lifecycle to investigate the effect a changing climate would have on the development of anthelmintic resistance. Climate data from six General Circulation Models based on four different Representative Concentration Pathways was available for three New Zealand locations. These projections were used to estimate …

The Effects Of Music On Dairy Production, Anneliese Kemp

Honors College Theses

The purpose of this research is to find which genre of music dairy cows produce the most milk to in order to aid dairy farmers in yielding maximum product while also keeping their cattle as comfortable as possible. During each lactation a different genre of music will play, basic vital signs, and behavioral observations of the cows will be taken, then the amount of milk produced during that genre will be measured. First, no music will be played, base line vitals will be taken, and milk yield will be measured. This will give an estimate to how positively or negatively …

The Surveillance Of Gastrointestinal Parasitic Nematodes Of Northwest Arkansas Dairy Cattle Using Traditional And Genetic Parasitological Identification Procedures, Eva M. Wray

Graduate Theses and Dissertations

Traditional and genetic parasitological identification procedures were compared using naturaland artificial nematode parasite infections in Holstein steer calves. The traditional parasitological procedures measured fecal egg counts, coprocultures with subsequent larval collection and adult nematodes collected at necropsy. The genetic identification procedures measured ITS-2 sequences extracted from different stages of nematode development: raw feces, concentrated nematode eggs, third stage larvae and adults. The primary nematodes observed were Cooperia oncophora, Cooperia punctata and Ostertagia ostertagi. The traditional techniques were not significantly different from one another, while the genetic sequencing showed variation amongst the different procedures. The raw feces sequences showed the most …

Comparative Analysis Of Intestinal Helminth Infections In Colic And Non-Colic Control Equine Patients, Heidrun Gehlen, Nadine Wulke, Antonia Ertelt, Martin K. Nielsen, Simone Morelli, Donato Traversa, Roswitha Merle, Douglas Wilson, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

All around the world, intestinal helminths constitute one of the most prevalent life-long occurring infections and re-infections affecting all horse age groups. A range of parasite species among strongyles, ascarids, and tapeworms is known to have the potential to cause colic in horses. However, there is a lack of current scientific evidence on the actual relevance of helminth infection levels in the context of colic in horses kept during prevailing epidemiological conditions. Thus, a prospective case-control study on the occurrence of intestinal helminths in a total of 620 mainly adult equine clinic patients was conducted to investigate the association between …

Meta-Analysis Of Cyathostomin Species-Specific Prevalence And Relative Abundance In Domestic Horses From 1975-2020: Emphasis On Geographical Region And Specimen Collection Method, Jennifer L. Bellaw, Martin K. Nielsen

Maxwell H. Gluck Equine Research Center Faculty Publications

BACKGROUND: Cyathostomins infect virtually all horses, and concomitant infections with 10 or more species per horse is standard. Species-specific knowledge is limited, despite potential species bias in development of disease and anthelmintic resistance. This is the first meta-analysis to examine effects of geographical region and cyathostomin collection method on reported composition of cyathostomin communities.

METHODS: Thirty-seven articles published in English in 1975 or later, in which adults of individual species were systematically enumerated, were included. Seven regions; North America, South America, eastern Europe, western Europe, northern Europe, southern Africa, and Oceania, and three cyathostomin collection methods; (i) standard necropsy recovery …

Importation Of Macrocyclic Lactone Resistant Cyathostomins On A Us Thoroughbred Farm, Martin K. Nielsen, M. Banahan, R. M. Kaplan

Maxwell H. Gluck Equine Research Center Faculty Publications

Anthelmintic resistance in equine cyathostomins is both widespread and highly prevalent in the benzimidazole and tetrahydropyrimidine classes; however, reports of resistance to macrocyclic lactone (ML) drugs are sparse and sporadic. This study reports a case of clear ML resistance in a group of Thoroughbred yearlings imported from Ireland to the US in 2019. Fecal egg count reduction (FECR) following ivermectin administered in February 2020 demonstrated 100% reduction in the US bred yearlings, but 93.5%, 70.5%, and 74.5% reduction in three groups of the imported yearlings. The two former groups were then retreated with ivermectin, yielding FECRs of 33.8% and 23.5%, …

The P-Glycoprotein Repertoire Of The Equine Parasitic Nematode Parascaris Univalens, Alexander P. Gerhard, Jürgen Krücken, Emanuel Heitlinger, I. Jana I. Janssen, Marta Basiaga, Sławomir Kornaś, Céline Beier, Martin K. Nielsen, Richard E. Davis, Jianbin Wang, Georg Von Samson-Himmelstjerna

Maxwell H. Gluck Equine Research Center Faculty Publications

P-glycoproteins (Pgp) have been proposed as contributors to the widespread macrocyclic lactone (ML) resistance in several nematode species including a major pathogen of foals, Parascaris univalens. Using new and available RNA-seq data, ten different genomic loci encoding Pgps were identified and characterized by transcriptome-guided RT-PCRs and Sanger sequencing. Phylogenetic analysis revealed an ascarid-specific Pgp lineage, Pgp-18, as well as two paralogues of Pgp-11 and Pgp-16. Comparative gene expression analyses in P. univalens and Caenorhabditis elegans show that the intestine is the major site of expression but individual gene expression patterns were not conserved between the two nematodes. In P. …

Metagenomic, Viral And Host Genetic Analyses Of Congenital Tremor In Pigs, Kylee Marie Sutton

School of Biological Sciences: Dissertations, Theses, and Student Research

The Pestivirus genus contains several viral species having a major impact on the livestock species. While there were only four major pestiviral species for a time, recent metagenomic sequencing approaches identified additional species, such as atypical porcine pestivirus (APPV). Congenital tremor was first identified almost a hundred years ago and since still has an impact on swine health. There are two main types of congenital tremor, type A and B, with type A congenital tremor further subcategorized based on causative agent. Until recently, type A-II congenital tremor did not have a known cause. Recent viral sequencing of affected samples, including …

The Efficacy Of Extended-Release Eprinomectin For The Reduction Of Horn Flies, Face Flies, And Fecal Egg Counts Of Parasitic Nematodes In Replacement Beef Heifers, Sophia F. Landers

Animal Science Undergraduate Honors Theses

The purpose of this study was to evaluate the efficacy of extended-release eprinomectin against horn flies, face flies, and fecal egg counts of parasitic nematodes in crossbreed replacement beef heifers. Fifty-four heifers were randomly placed into three treatment groups (N=18 heifers/treatment). Group 1 was administered the labeled dosage of extended-release eprinomectin on day 0. Group 2 acted as the negative control. Group 3 received the anthelmintic injection once a quarter of the heifers in the group reached the threshold treatment level for horn flies (N=200 flies/animal; day 41). Nematode infections were measured via fecal egg counts while horn and face …