Large or Food Animal and Equine Medicine Commons^™

Effects Of Feeding Microbial Feed Additives On Growth Performance And Carcass Traits Of Steers Fed Steam-Flaked Corn-Based Diets With Wet Distillers Grains Plus Solubles, Matt K. Luebbe, Karla H. Jenkins, Stephanie A. Furman Furman, Kelly K. Kreikemeier

Nebraska Beef Cattle Reports

An experiment was conducted to determine the effects of feeding two commercially available direct-fed microbials (DFM) on finishing steer performance fed steam-flaked corn based diets. Dietary treatments included a control diet without DFM, and two commercially available products (10-G and Bovamine). No significant differences were observed among treatments for animal performance or carcass characteristics. However, numeric advantages were observed for ADG and feed efficiency when cattle were fed a DFM.

Effects Of Feeding 44 G/Ton Rumensin® During Grain Adaptation On Animal Performance And Carcass Characteristics, Cody J. Schneider, Matt K. Luebbe, Karla Jenkins Jenkins, Stephanie A. Furman, Galen E. Erickson

Nebraska Beef Cattle Reports

Cattle were adapted to a finishing diet over 20 days while feeding 33 or 44 g/ton (DM) of Rumensin. Following grain adaptation, all cattle were fed a common finishing diet containing 33 g/ton Rumensin for the remainder of the feeding period. Feeding 44 g/ton of Rumensin during the adaptation period did not affect performance during the adaptation period or over the entire feeding period when compared to the 33 g/ton adaptation level.

Effect Of Winter Supplementation Level On Yearling System Profitability, Kari L. Gillespie, Brandon L. Nuttelman, Cody J. Schneider Schneider, Terry J. Klopfenstein

Nebraska Beef Cattle Reports

Calves backgrounded in a forage-based system at a high winter supple- mentation level maintained a performance advantage through finishing. High level supplemented cattle gained an additional 0.2 lb. daily during finishing, consumed less total feed in the feedlot, required fewer days on feed to reach a common finish point and produced an additional 85 lb. of saleable live weight compared to cattle backgrounded at a low supplementation level. High level supplemented cattle returned $56.01 more than cattle fed a low level of supplementation during the winter backgrounding phase.

Structure-Function Analysis Of A Protein Encoded By The Bhv-1 Latency Related Gene, Devis Sinani

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Bovine herpes virus 1 (BHV-1) is a significant viral pathogen in cattle that induces a myriad of clinical symptoms. These symptoms include: conjunctivitis, upper respiratory tract infections, genital disorders, and abortions. BHV-1 infection can also lead to transient immune-suppression, which predisposes cattle to secondary bacterial infection leading to life-threatening pneumonia referred to as bovine respiratory disease (BRD). Following acute infection, BHV-1 establishes latency in sensory neurons within trigeminal ganglia. Reactivation of the virus can occur periodically, resulting in virus transmission. The latency-related (LR) RNA is the only abundantly expressed transcript in latently infected sensory neurons and it encodes several proteins, …

A Toxicology And Clinical Study Of Post Race Epitaxis Associated With Exercise Induced Pulmonary Hemorrhage In Thoroughbred Race Horses At The Racecourse Rinconada, Caracas, Venezuela, Abelardo Morales Briceno, Diana Villoria Leon, Kimberly Brewer, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

The aim of this study was to describe cases of epistaxis associated with post-race Exercise Induced Pulmonary Hemorrhage (EIPH) in Thoroughbred horses at the Hippodrome "La Rinconada", Caracas, Venezuela, through a clinic pathological study. We studied a total of 29 cases of epistaxis post-race in Thoroughbred horses at the Hippodrome La Rinconada, Caracas, Venezuela, which is 2,950 meters above sea level. The study included horses between the ages of 2-5 years, 16 stallions and 13 mares, weighing between 450-510 kg. They underwent a clinical examination, although horses presenting with epistaxis were in an emergency situation. Samples of blood and urine …

Breeding Induced Endometritis In The Mare: The Local Innate Immune Response, Elizabeth Moran Woodward

Theses and Dissertations--Veterinary Science

Uterine inflammation after breeding is considered necessary for the clearance of excess semen and debris from the uterus. A subpopulation of mares fails to clear the inflammation in a timely fashion, and develops a persistent breeding induced endometritis (PBIE). Experiments were preformed to evaluate correlations of PBIE to endometrial quality and age. Mares of advanced age and poor endometrial quality had a higher incidence of PBIE. In addition, mares fluctuated in susceptibility to PBIE from one season to the next. The uterine inflammatory gene expression in susceptible and resistant mares within the first 24 hours after breeding was investigated. The …

Surveillance Of Selected Diseases In Free-Ranging Elk (Cervus Elaphus Nelsoni) In Nebraska, 1995-2009, Michael A. Cover, Scott E. Hygnstrom, David W. Oates, Kit M. Hams, Kurt C. Vercauteren

Great Plains Research: A Journal of Natural and Social Sciences (through 2013)

Sera samples were collected from 21 free-ranging, captured female elk (Cervus elaphus nelsoni) in 1995- 96, and tissue and sera samples were collected from 415 hunter-harvested elk from 1995 to 2006 and tested for selected diseases. Titers for Anaplasma marginale were detected in 81 of 436 (19%) elk. Occurrence of antibodies to anaplasmosis increased from 4 to 40 elk from 2002 to 2006. Titers for bovine viral diarrhea virus (BVDV) were detected in 18 of 346 (5%) samples. Titers for Leptospira interrogans serovars were detected in 21 of 289 (7%) of samples from 1995 to 2004. Titers for …

Caring For Compromised Cattle: Assessing Animals At Risk

Nebraska Beef Quality Assurance Program

Cattlemen have long recognized the need to properly care for their livestock. Sound animal husbandry practices – based on research and decades of practical experience – are known to impact the well-being of cattle, individual animal health and herd productivity. There is no doubt that producers work hard to ensure that their cattle are properly cared for throughout all stages of production. Unfortunately, it’s a fact of raising cattle that some animals will become injured to the extent that they are unable to stand or walk without assistance.

Assessor’S Guide To A Beef Quality Assurance Cow-Calf Assessment

Nebraska Beef Quality Assurance Program

The BQA Cow-Calf Assessment is an on-site educational tool that allows for assessing and benchmarking key indicators of animal care and well-being as well as operational conditions. The Cow-Calf Assessment focuses on three main areas – Animals, Records and Best Management Practices (BMP), and Facilities and Equipment.

2011 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow/Calf

• Winter Grazing System and Supplementation of Beef Cows During Late Gestation Influence Heifer Progeny
• Influencing Steer Performance Through Maternal Nutrition
• Integration of DNA Marker Information into Breeding Value Predictions
• Granulosa Cell Gene Expression is Altered in Follicles from Cows with Differing Reproductive Longevity

Growing

• Performance of Growing Cattle Fed Corn Silage or Grazing Corn Residue from Second Generation Insect-protected (MON 89034), Parental, or Reference Corn Hybrids
• Nutrient Composition of Spoiled Wet Byproducts Mixed and Stored with Straw
• Use of Dry Rolled Corn, Dry or Wet distillers Grains Plus Solubles as an Energy Source in High Forage Diets for Growing …

Evaluation Of Mass Spectrometric Methods For Detection Of The Anti-Protozoal Drug Imidocarb, A. F. Lehner, J. A. Hitron, J. May, C. Hughes, R. Eisenberg, N. Schwint, D. P. Knowles, P. Timoney, Thomas Tobin

Maxwell H. Gluck Equine Research Center Faculty Publications

Imidocarb [N,N'-bis[3-(4,5-dihydro-1H-imidazol-2-yl)phenyl]urea, C₁₉H₂₀N₆O₁, m.w. 348.41] is a symmetrical carbanilide derivative used to treat disease caused by protozoans of the Babesia genus. Imidocarb, however, is also considered capable of suppressing Babesia-specific immune responses, allowing Babesia-positive horses to pass a complement fixation test (CFT) without eliminating the infection. This scenario could enable Babesia-infected horses to pass CFT-based importation tests. It is imperative to unequivocally identify and quantify equine tissue residues of imidocarb by mass spectrometry to address this issue. As a pretext to development of sensitive tissue assays, we have investigated possibilities of mass …

Development, Optimization, And Validation Of A Classical Swine Fever Virus Real-Time Reverse Transcription Polymerase Chain Reaction Assay, August J. Eberling, Jill Bieker-Stefanelli, Monica M. Reising, David Siev, Barbara M. Martin, Michael T. Mcintosh, Tammy R. Beckham

Zoonotics and Wildlife Disease: Publications

Classical swine fever (CSF) is an economically devastating disease of pigs. Instrumental to the control of CSF is a well-characterized assay that can deliver a rapid, accurate diagnosis prior to the onset of clinical signs. A real-time fluorogenicprobe hydrolysis (TaqMan) reverse transcription polymerase chain reaction (RT-PCR) for CSF was developed by the United States Department of Agriculture (USDA) at the Plum Island Animal Disease Center (CSF PIADC assay) and evaluated for analytical and diagnostic sensitivity and specificity. A well-characterized panel including Classical swine fever virus (CSFV), Bovine viral diarrhea virus (BVDV), and Border disease virus (BDV) isolates was utilized in …

Regulation Of The Latency–Reactivation Cycle By Products Encoded By The Bovine Herpesvirus 1 (Bhv-1) Latency-Related Gene, Clinton Jones, Leticia Frizzo Da Silva, Devis Sinani

School of Veterinary and Biomedical Sciences: Faculty Publications

Like other α-herpesvirinae subfamily members, the primary site for bovine herpesvirus 1 (BHV-1) latency is ganglionic sensory neurons. Periodically BHV-1 reactivates from latency, virus is shed, and consequently virus transmission occurs. Transcription from the latency-related (LR) gene is readily detected in neurons of trigeminal ganglia (TG) of calves or rabbits latently infected with BHV-1. Two micro-RNAs and a transcript encompassing a small open reading frame (ORF-E) located within the LR promoter can also be detected in TG of latently infected calves. A BHV-1 mutant that contains stop codons near the beginning of the first open reading frame (ORF2) within the …

Tactics For Identifying And Eliminating Tritrichomonas Foetus From Infected Beef Herds, Jeff D. Ondrak

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

The protozoan, Tritrichomonas foetus (TF), has been recognized as a cause of bovine infertility for more than 100 years (Skirrow and BonDurant, 1988). As an obligate parasite of the bovine reproductive tract its control and eradication seems achievable (Harding, 1950). However, this disease continues to trouble United States cattle producers and a recent epidemic in the Western US has lead to increased interest in research and regulatory efforts (Cima, 2009). Outbreak investigations were carried out on three Nebraska ranches to assess the efficiency of currently available diagnostic tests, culture, gel polymerase chain reaction (PCR), and real time PCR (rtPCR), in …

Dairy Animal Care: Quality Assurance

Nebraska Beef Quality Assurance Program

Dairy producers are encouraged to participate in the voluntary Dairy Animal Care and Quality Assurance (DACQA) Certification program. The purpose of this program is to enhance and demonstrate quality animal care practices, which assure food safety, quality and value as well as enhance consumer confidence in the milk and beef products that are harvested from cattle on America’s dairy farms.

School Of Veterinary Medicine & Biomedical Sciences: 2010 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

On September 4, 2009, The Department of Veterinary Medicine and Biomedical Sciences was renamed the School of Veterinary Medicine and Biomedical Sciences. The School is informally organized into six functional areas: • Undergraduate Teaching • Research and Graduate Studies • Extension • Veterinary Diagnostic Center (VDC) • Great Plains Veterinary Educational Center (GPVEC) • Professional Program in Veterinary Medicine (PPVM) While teaching and research activities bridge the entire faculty, subsets of the faculty are primarily involved with one area, i.e., the undergraduate program, the research and graduate program, the veterinary medical students clinical teaching at the GPVEC, the pre-clinical teaching …

Gene Delivery In The Equine Cornea: A Novel Therapeutic Strategy, Dylan G. Buss, Ajay Sharma, Elizabeth A. Giuliano, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To determine if hybrid adeno-associated virus serotype 2/5 (AAV5) vector can effectively deliver foreign genes into the equine cornea without causing adverse side effects. The aims of this study were to: (i) evaluate efficacy of AAV5 to deliver therapeutic genes into equine corneal fibroblasts (ECFs) using enhanced green fluorescent protein (EGFP) marker gene and (ii) establish the safety of AAV5 vector for equine corneal gene therapy.

Animal Material—Primary ECF cultures were harvested from healthy donor equine corneas. Cultures were maintained at 370C in humidified atmosphere with 5% CO2.

Procedure—AAV5 vector expressing EGFP under control of hybrid cytomegalovirus (CMV) + chicken …

Isolation And Cultivation Of Equine Corneal Keratocytes, Fibroblasts And Myofibroblasts, Dylan G. Buss, Elizabeth A. Giuliano, Ajay Sharma, Rajiv R. Mohan

Pharmacy Faculty Articles and Research

Objective—To establish an in vitro model for the investigation of equine corneal wound healing. To accomplish this goal, a protocol to isolate and culture equine corneal keratocytes, fibroblasts and myofibroblasts was developed.

Animal material—Equine corneal buttons were aseptically harvested from healthy research horses undergoing humane euthanasia for reasons unrelated to this study. Slit-lamp biomicroscopy was performed prior to euthanasia by a board-certified veterinary ophthalmologist to ensure that all samples were harvested from horses free of anterior segment disease.

Procedure—Equine corneal stroma was isolated using mechanical techniques and stromal subsections were then cultured. Customized media at different culture conditions was used …