Natural Products Chemistry and Pharmacognosy Commons^™

Penetapan Kadar Fenolik Dan Flavonoid Total Ekstrak Metanol Buah Dan Daun Patikala (Etlingera Elatior (Jack) R.M.Sm), Aktsar Roskiana Ahmad, Juwita Juwita, Siti Afrianty Daniya Ratulangi

Pharmaceutical Sciences and Research

Fruit and leaves of patikala (Etlingera elatior (Jack) R.M.Sm), is a traditional medicinal plants used by people to treat various diseases. The purpose of this study was to determine the total phenolic and flavonoid compounds of E. elatior fruits and leaves extracts. We extracted dried fruits and leaves samples by maceration method using methanol. Qualitative analysis of chemical compounds with certain eluent was done using Thin Layer Chromatography (TLC) to determine the group of active compounds in the extract. The amount of phenolic compounds in the sample was determined by the colorimetric method at a maximum wavelength of 744.8 nm …

Losio Antioksidan Buah Naga Merah (Hylocereus Polyrhizusbritton And Rose), Amanda Angelina Sinaga, Sri Luliana, Andhi Fahrurroji

Pharmaceutical Sciences and Research

Hylocereus polyrhizus Britton and Rose (Red dragon fruit) has been shown to have antioxidant activity which contains vitamin C, polyphenol, and flavonoid. This research was aimed to investigate antioxidant activity from H. polyrhizus in form of lotion. Lotion were made with 5 concentration from H. polyrhizus methanol extract were 0.04, 0.08, 0.16, 0.32 and 0.64%. Lotion antioxidant activity was measured using DPPH method. The result showed that H. polyrhizus methanol extract had antioxidant activity with inhibition concentration were 19.99±0.33; 25.01±0.08; 39.14±0.04; 66.69±0.12 and 83.37±0.05. The result showed significant differences on antioxidant activity. The physical stability observation of five formula with …

Pembentukan 8-Ohdg Dari Zat Toksik Pemicu Radikal Bebas, Intan Cahaya Dani, Budiawan Budiawan

Pharmaceutical Sciences and Research

This study was conducted to observe the profile of DNA Adduct (8-OHdG) formation as DNA damage indicators, by using calf thymus DNA incubated with toxic and carcinogenic compounds. The compounds which could trigger free radicals in this research were PAH(Benzo[a]Pyrene), TiO2, and CuCl2. Calf thymus DNA was incubated with Benzo[a]Pyrene and CuCl2 compounds under pH and temperature variations. The incubation of calf thymus DNA with TiO2-UV radiation (254 nm) was used to induce the formation of reactive oxygen species (ROS) in the process of oxidative DNA damage. From this research, all of compounds have potency to trigger the formation of …

Sintesis Ramah Lingkungan Senyawa Imina Turunan Vanilin Dan 2-Hidroksi Asetofenon Serta Uji Aktivitas Biologi Dan Antioksidan, Herry Cahyana, Putri Pratiwi

Pharmaceutical Sciences and Research

Eco-friendly synthesis of imine derivative compound from 2-hydroxy acetophenone and vanillin had been conducted by using a method of stirring (with stirrer) in a water solvent. The whole experiment began with the synthesis of imine compounds, followed by purification and characterization of the synthesis products and finally analysis on their biological and antioxidant activities. The experiments showed that imine compounds could be synthesized by a chemical reaction of 4-amino antipirin aqueous solution with 2-hydroxy acetophenone and vanillin at magnetic stirrer speed of 250 and 450 rpm, respectively, which produced product A, (E)-4-(1-(2-hydroxyphenyl) ethylideneamino)-1,5-dimethyl-2-phenyl-1H-pyrazol-3(2H) -one and product B, (E)-4-(4-hydroxy-3-(vinyloxy) benzylideneamino)-1,5-dimethyl-2-phenyl-1H-pyrazol-3(2H)-one. Yield …

Evaluasi Kadar Kurkumin Dalam Jamu Tradisional Kunir Asam Yang Dijual Di Pasar Kota Gede Bulan Februari 2015, Fitrilya Mustikahati Yusuf, Nurkhasanah Nurkhasanah

Pharmaceutical Sciences and Research

Javanese women often consume kunir asam to relieve dysmenorrhea symptom. It is a traditional herbal medicine which is conventionally prepared from curcuma domestica. One of the active compound of curcuma domestica is curcumin. The purpose of this research is to determine the amount of curcumin available in kunir asam selling in daily market of Kota Gede. Curcumin level in daily batches consistency was also evaluated. Curcumin analysis was conducted by qualitative and quantitative tests by TLC densitometry method. A total of 50.0 ml kunir asam was extracted with 25.0 ml of chloroform, and then the mixture was dried by evaporation. …

Toksisitas Ekstrak Etanol Mangifera Foetida L. Sebagai Pengkelat Besi Ditinjau Dari Ld50 Dan Komponen Sel Darah, Tri Wahyuni, Santi Purna Sari, Ari Estuningtyas, Hj Freisleben

Pharmaceutical Sciences and Research

Ethanolic extract of bacang mango leaves (Mangifera foetida L.) decreased iron concentration in blood Sprague dawley rats that had been induced iron overload. The aim of this experiment was to determine acute toxicity (LD50) value of ethanolic extract of M.foetida by weil method and its effect to blood component. This study was conducted by employing a complete random design using 25 male and 25 female mice of DDY strain which devided into 5 groups. The first group until fourth group were administered ethanolic extract of M.foetida with dose variation which suspension in aquadest orally. The fifth group was control that …

Evaluasi Aktivitas Antioksidan Senyawa 4-[(E)-2-(4-Okso-3-Fenilkuinazolin-2-Il)Etenil]-Benzensulfonamida Dan Analognya, Catur Jatmika, Baitha Palanggatan Maggadani, Hayun Hayun

Pharmaceutical Sciences and Research

Quinazolinone derivative compounds exhibit a broad spectrum of biological activity, including antioxidant. Quinazolinone derivative compound 4-[(E)-2-(4-oxo-3-phenyl quinazolin-2-yl)ethenyl]-benzensulfonamida and its analogs (2a-f) have been synthesized and demonstrated its activity as a selective inhibitor of cyclooxygenase-2. The aim of this research was to evaluate the antioxidant activity of compound 4-[(E) -2-(4-oxo-3-fenilkuinazolin-2-yl)ethenyl]-benzensulfonamida and its analogs with DPPH radical scavenging method, ferric reducing activity potential (FRAP), and phosphomolybdenum method. The experiment revealed that the % inhibition and antioxidant capacity of compound 2c, 2e and 2f were higher compared to compound 2a, 2b and 2d. The DPPH radical scavenging analysis found that the % inhibition …

Sintesis Dan Karakterisasi Hidrogel Superabsorben Kitosan Poli(N-Vinilkaprolaktam) (Pnvcl) Dengan Metode Full Ipn (Interpenetrating Polymer Network), Nadhrah Wivanius, Emil Budianto

Pharmaceutical Sciences and Research

Polymer material used for hydrogel should have capability to swell and to keep water molecules in its structure without solubilised in water. Natural polymers has functional groups which can perform as active sites in modification to produce polymer with better characteristic. Chitosan is a natural polymer which has good swelling ability but lack of structural strength. Synthesis of chitosan hydrogel by interpenetrating polymer network (IPN) will increase its strength through crosslinking. In this research, the first step of modification was the synthesis of chitosan polymer network crosslinked by acetaldehyde. The next step was the synthesis of PNVCL polymer network crosslinked …

Curcumin Induces Apoptosis Of Upper Aerodigestive Tract Cancer Cells By Targeting Multiple Pathways, Arm R. Amin, A. Haque, M. A. Rahman, Z. G. Chen, F. R. Khuri, D. M. Shin

Pharmaceutical Science and Research

Curcumin, a natural compound isolated from the Indian spice "Haldi" or "curry powder", has been used for centuries as a traditional remedy for many ailments. Recently, the potential use of curcumin in cancer prevention and therapy urges studies to uncover the molecular mechanisms associated with its anti-tumor effects. In the current manuscript, we investigated the mechanism of curcumin-induced apoptosis in upper aerodigestive tract cancer cell lines and showed that curcumin-induced apoptosis is mediated by the modulation of multiple pathways such as induction of p73, and inhibition of p-AKT and Bcl-2. Treatment of cells with curcumin induced both p53 and the …

Fda Approves 18 New Molecular Entities/Biologics, Rodney Richmond

Faculty Research ​and Publications

No abstract provided.

Target-Directed Biosynthetic Evolution: Redirecting Plant Evolution To Genomically Optimize A Plant’S Pharmacological Profile, Dustin Paul Brown

Theses and Dissertations--Neuroscience

The dissertation describes a novel method for plant drug discovery based on mutation and selection of plant cells. Despite the industry focus on chemical synthesis, plants remain a source of potent and complex bioactive metabolites. Many of these have evolved as defensive compounds targeted on key proteins in the CNS of herbivorous insects, for example the insect dopamine transporter (DAT). Because of homology with the human DAT protein some of these metabolites have high abuse potential, but others may be valuable in treating drug dependence. This dissertation redirects the evolution of a native Lobelia species toward metabolites with greater activity …

Design, Synthesis, And Pharmacological Evaluation Of Three Series Of Lobelane Analogs As Inhibitors Of The Vesicular Monoamine Transporter (Vmat2), John P. Culver

Theses and Dissertations--Pharmacy

Methamphetamine (METH) abuse is a serious problem in the United States and worldwide. The reward experienced by METH users is due to the increase in extracellular dopamine (DA) concentrations caused by an interaction between METH and the DA transporter (DAT) as well as the Vesicular Monoamine Transporter-2 (VMAT2). The reward felt by users of METH leads to further use of the drug and subsequent abuse. The current project examined the ability of three novel series of lobelane analogs to interact with a binding site on the Vesicular Monoamine Transporter-2 (VMAT2) in an attempt to inhibit the effects of METH. Lobelane …

Chemoenzymatic Studies To Enhance The Chemical Space Of Natural Products, Jhong-Min Chen

Theses and Dissertations--Pharmacy

Natural products provide some of the most potent anticancer agents and offer a template for new drug design or improvement with the advantage of an enormous chemical space. The overall goal of this thesis research is to enhance the chemical space of two natural products in order to generate novel drugs with better in vivo bioactivities than the original natural products.

Polycarcin V (PV) is a gilvocarcin-type antitumor agent with similar structure and comparable bioactivity with the principle compound of this group, gilvocarcin V (GV). Modest modifications of the polyketide-derived tetracyclic core of GV had been accomplished, but the most …

Antibiotics Targeting Tuberculosis: Biosynthesis Of A-102395 And Discovery Of Novel Actinomycins, Wenlong Cai

Theses and Dissertations--Pharmacy

The increase in antibiotic resistance of many bacterial strains including multidrug-resistant tuberculosis (MDR-TB) due to over- and misuse of antibiotics is a serious medical and economical problem. Therefore discovery and development of new antibiotics are urgently needed. Two projects were undertaken to address the need for new anti-tuberculosis antibiotics.

1. Discovery of new chemical entities. A-102395, a new nucleoside inhibitor of bacterial MraY (translocase I, EC 2.7.8.13) that is essential for bacterial survival, was isolated from the culture broth of Amycolatopsis sp. SANK 60206 in 2007. Although A-102395 is a potent inhibitor of translocase I with IC50 of 11 nM, …

Elucidating The Mechanism Of Lipl: A Non-Heme Fe(Ii), Α -Ketoglutarate: Uridine-5’-Monophosphate Dioxygenase, Anwesha Goswami

Theses and Dissertations--Pharmacy

Several nucleoside natural product antibiotics from Streptomyces sp. and actinomycetes have recently been shown to target bacterial peptidoglycan cell wall biosynthesis by inhibiting the bacterial translocase I (MraY). The biosynthetic gene clusters for A-90289, liposidomycins and caprazamycins revealed a protein with sequence similarity to proteins annotated as α-KG:taurine dioxygenases (TauD). This enzyme (LipL) is a mononuclear, non-heme, Fe(II) dependent α-keto glutarate (α-KG) :uridine monophosphate (UMP) dioxygenase responsible for the net dephosphorylation and two electron oxidation of UMP to uridine-5’-aldehyde. The postulated reaction coordinates involving the activation of the C-5’ center in UMP and the corresponding formation of uridine-5’-aldehyde are modeled …

Feasibility Of Integrating Tripterygium Wilfordii Into Modern Cancer Therapy For Increased Efficacy And Minimal Toxicity, Ngoc T. Vo

UROP Posters

Cancer is the second leading cause of death in the U.S. and millions of novel cancer cases are being diagnosed each year. While chemotherapy and ionizing radiation are effective treatments against these malignant tumors, the adverse effects that accompany such treatments are devastating. In order to find alternative treatment methods with less side effects, we turn to Eastern herbal medicine. Recent scientific research has found that Tripterygium wilfordii, an herbal medicine traditionally used to treat inflammation in China, contains compounds (triptolide and celastrol) that prevent the growth of solid tumors, induce apoptosis, and prevent metastasis of developed tumors. Investigations …

Efek Fraksi N-Heksana Kulit Hylocereus Polyrhizus Terhadapaktivitas Katalase Tikus Stres Oksidatif, Eka Kartika Untari, Sri Wahdaningsih, Agustia Damayanti

Pharmaceutical Sciences and Research

Production of excess free radicals cause oxidative damage and degenerative diseases. Catalase enzyme is one of the endogenous intracellular antioxidant that can be reduced by oxidative stress conditions. The peel of red dragon fruit (Hylocereus polyrhizus) as a potent exogenous antioxidants could support the internal enzyme activity to prevent free radicals. The aim of this research was to determine the effect of n-hexane fraction of H.polyrhizus’s peel and the dose that could increase activity of catalase. The peel was maserated with chloroform, then fractionated by n-hexane. Stress condition was achieved by five days fasting and swimming for 10 mins/day to …

Efek Fraksi N-Heksana Kulit Hylocereus Polyhizus Terhadap Kadar Malondialdehida Tikus Stres Oksidatif, Wirna Maya Sari, Sri Wahdaningsih, Eka Kartika Untari

Pharmaceutical Sciences and Research

Excessive free radical production will lead to oxidative stress. One of the indicators used to determine stress oxidative is malondialdehyde (MDA). Hylocereus polyhizus peel is potential used natural exogenous antioxidant. The aim of this research was to study the effect of n-hexane fraction of H.polyhizus peel on MDA level and to determine the dose that could reduce the MDA level. H.polyhizus peel was macerated with chloroform, fractionated with n-Hexane. Rats were divided into 7 groups : normal, negative, positive control vitamin E (18 mg/ kgbw), positive control quercetin (4 mg/200gbw), H.polyhizus peel extract group treated with dose I (10mg/ 200gbw), …

Formulasi Krim Antioksidan Ekstrak Etanol Daun Ubi Jalar Ungu (Ipomoea Batatas (L.) Lamk) Sebagai Anti Aging, Damaranie Dipahayu, Widji Soeratri, Mangestuti Agil

Pharmaceutical Sciences and Research

No abstract provided.

Antibakteri Fraksi N-Heksana Kulit Hylocereus Polyrhizus Terhadap Staphylococcus Epidermidis Dan Propionibacterium Acnes, Sri Wahdaningsih, Eka Kartika Untari, Yunita Fauziah

Pharmaceutical Sciences and Research

Acne can be caused by Staphylococcus epidermidis and Propionibacterium acnes. By using antibiotic for the acne theraphy in a long term period can cause resistance, organ detriment and imunohypersensitivity. Red dragon fruit’s peel (Hylocereus polyrhizus) is one of natural materials which can be used for alternative antibacterial. This study aims to determine the antibacterial activity of n-Hexane fraction of red dragon fruit’s peel against S. epidermidis dan P. acnes by the method of disc diffusion Kirby-Bauer. Red dragon fruit’s peel powder macerated within chloroform, and macerate then fractionated with n-Hexane. Phytochemical screening result red dragon fruit’s peel contains terpenoid and …