Dentistry Commons^™

The Role Of Human Msc Derived Exosomes In The Treatment Of Periodontal Diseases, Sonia S. Talegaonkar

Theses and Dissertations

Periodontal disease affects 47% of Americans over 30. Characterized by microbial dysbiosis and unregulated inflammation, severe periodontitis causes degradation of bone and soft tissue around teeth. Current treatments have limited regenerative outcomes and frequent reinfection by harmful bacteria. Human mesenchymal stem cells (hMSCs) have been shown to promote wound healing and tissue regeneration. Many therapeutic benefits of hMSCs are due to their secretome products, like exosomes. Our long-term goal is to develop periodontal therapies with hMSC exosomes. The objectives of this study were to determine the effect of hMSC-derived exosomes on cellular activity of hMSCs and investigate whether hMSC exosome …

A Comparative Study Of Intraradicular Enterococcus Faecalis Biofilm Removal With Three Root Canal Treatment Systems: A Scanning Electron Microscopy Evaluation, Cyrous Ardalan

Theses and Dissertations

The objective of this study was to evaluate the biofilm removal efficacy of three root canal treatment systems: ProUltra® PiezoFlow™, traditional needle irrigation, and the GentleWave® system in an ex-vivo benchtop study. Twenty-four extracted maxillary and mandibular molars were selected. Teeth were all instrumented to a master apical file size #25 with 4% taper. Teeth were then randomly divided into four experimental groups and two control groups. The root canals were inoculated with a culture of Enterococcus faecalis and incubated for five weeks to form a biofilm. Each group was then treated with one of the different root canal treatment …

The Prevalence, Predictive Factors, And Classification Of Intrapulpal Cracks In Maxillary Molars Requiring Endodontic Treatment, Husain Karashi

Theses and Dissertations

Diagnosis and treatment of teeth with longitudinal fractures is challenging. Cracks are usually not visible radiographically; they require a thorough evaluation to aid in diagnosis. Patients may be asymptomatic, demanding the dentist rely on clinical findings to make a diagnosis. Early diagnosis of the presence and extent of a crack is essential for the successful management of a cracked tooth. There is limited information in the literature regarding the prevalence or predictive factors of cracks extending into the pulp chamber of teeth. The purpose of this study was to determine the prevalence of intrapulpal cracks in maxillary molars and to …

Orthodontists' And Parents' Perspective Of Occlusion In Varying Anterior-Posterior Positions: A Comparative Study, David H. Lindsey

Theses and Dissertations

Objective: The purpose was to compare orthodontists’ and parents’ perception of orthodontic treatment outcomes in the anterior-posterior (AP) dimension. Assessment of treatment time and compliance were also investigated.

Material and Methods: Parallel surveys for orthodontists (n=1000) and parents (n=750) displayed occlusions from 3 mm Class III (Cl III:3) to 3 mm Class II. Participants rated occlusal relationships on a 100 mm VAS from least to most acceptable (0-100).

Results: 233 orthodontists (23%) and 243 parents (32%) responded. Orthodontists (mean=93.9, 25.9) and parents (mean=80.7, 40.9) rated Class I (Cl I) occlusion most and Cl III:3 least acceptable. No significant difference was …

Work-Related Musculoskeletal Disorders Among Dentists And Orthodontists, Natalie R. La Rochelle

Theses and Dissertations

WORK-RELATED MUSCULOSKELETAL DISORDERS AMONG DENTISTS AND ORTHODONTISTS

A thesis submitted in partial fulfillment of the requirements for the degree of

Master of Science in Dentistry at Virginia Commonwealth University.

by

Natalie R. La Rochelle

Thesis Director: Dr. Eser Tüfekçi, D.D.S., M.S., Ph.D., M.S.H.A.

Professor, Department of Orthodontics

Virginia Commonwealth University

Richmond, Virginia

May 2017

The practice of dentistry is physically demanding due to static and dynamic postures sustained daily throughout careers. Previous literature suggests that work-related musculoskeletal disorders (WMSD) are not solely the result of work habits, but also due to the individual, his or her physical makeup, genetics, and …

A Comparison Of Moderate Oral Sedation Drug Regimens For Pediatric Dental Treatment: A Pilot Study, Ojas A. Parikh

Theses and Dissertations

Purpose: Compare moderate oral sedation of pediatric patients using Hydroxyzine and Meperidine with either Diazepam or Midazolam in management of pediatric dental patients.

Methods: Randomized, double-blind, crossover pilot study of patients 3 to 7 years of age requiring two sedation visits. Frankl and Houpt behavior scores recorded at injection time, initiation of treatment and 100% oxygen at end of treatment. Postoperative phone call surveys conducted within eight hours and within 24 hours of discharge. Wilcoxon Signed-Rank tests, Fisher’s Exact Chi-squared test and 0.10 significance level.

Results: 25 subjects completed 35 sedations. Eight participants completed both treatments and demonstrated significantly higher …

Amixicile Inhibits Anaerobic Bacteria Within An Oral Microbiome Derived From Patients With Chronic Periodontitis, Kane Ramsey

Theses and Dissertations

Periodontitis is a chronic inflammatory disease caused by pathogenic bacteria residing in a complex biofilm within a susceptible host. Amixicile is a non-toxic, readily bioavailable novel antimicrobial that targets strict anaerobes through inhibition of the activity of Pyruvate Ferredoxin Oxidoreductase (PFOR), a major enzyme mediating oxidative decarboxylation of pyruvate. Our study aimed to evaluate the efficacy of amixicile, when compared to metronidazole, in inhibiting the growth of bacteria present in a microbiome harvested from patients with chronic periodontitis.

Plaque samples were harvested from patients with severe chronic periodontitis and cultured under anaerobic conditions. The microbiomes were grown in the presence …

Provider Appearance: A Survey Of Guardian And Patient Preference, Joshua Westphal

Theses and Dissertations

Purpose: To understand guardian and child preferences for the appearance of their pediatric dentist. This was a cross-sectional descriptive study using survey methodology with patients and parents that attended the VCU Pediatric Dental clinic.

Methods: A total sample of 100 guardians and 97 pediatric patient participants completed the computer-based questionnaire. Four subjects were asked to pose for photographs wearing various combinations of attire (professional, casual, white coat, scrubs).

Results: Among guardians, 56% reported preferring a provider in scrubs, with white coat the second most preferred attire (39%) for their children. For pediatric patients, scrubs were still most often selected, …

Evaluation Of Push Out Bond Strength Of Glass Fiber Post Related With Ultrasonic Irrigation, Nomjit Vidhyaphum

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective This study compared the bond strength of glass fiber post to dentin using different irrigation methods and solutions and to evaluate the bond strength in different regions of the root. Methods One hundred human anterior maxillary teeth were randomly divided into 10 groups (n=10). Root canal filling and post space preparation were carried out followed by diverse final irrigation techniques. Groups 1 to 3 underwent syringe irrigation, groups 4 to 6 irrigation with ultrasonic instrumentation and groups 7 to 9 passive ultrasonic irrigation. Different irrigant solutions were used in each groups as 2.5% NaOCl (groups 1,4,7), 17% EDTA (groups …

Microleakage Of Enamel Preservation At Gingival Wall Of Class Ii Resin Composite Restorations (Nanofilled Vs Bulk-Fill), Onladda Pisuttiwong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives. This in vitro study tested the hypothesis that preserving a thin enamel layer at the gingival margin and using bulk-fill resin composites could minimize microleakage of class II resin composite. Materials and Methods. Thirty-six human third molars were randomly divided into three groups of 12 specimens each: Filtek Bulk Fill Posterior Restorative in Capsules (BFC), Filtek Bulk Fill Posterior Restorative in Syringes (BFS) and Filtek Z350 XT (Z350). Teeth were prepared on two sides for a class II cavity (3 mm buccolingually x 2 mm mesiodistally at occlusal and 1.5 mm at coronal x 4 mm of axial depth) …

Effect Of Remineralization On Color Change Of Bleached Tooth, Panita Suttisakpakdee

Chulalongkorn University Theses and Dissertations (Chula ETD)

The aim of this in vitro study was to investigate the effect of remineralization and time on the color and microhardness change of teeth bleached using 10% carbamide peroxide (10%CP) or 40% hydrogen peroxide (40%HP). Seventy-two 6x6x2 mm3 enamel slabs were prepared from human premolars. The specimens' color and Vickers' microhardness were recorded at baseline (T0). The specimens were divided into six groups, two groups were treated with 10%CP with or without casein phosphopeptides and amorphous calcium phosphate (CPP-ACP) (10%CP and 10% CP/CPP-ACP, respectively), two groups were treated with 40% HP with or without CPP-ACP (40%HP and 40%HP/CPP-ACP, respectively), one …

The Overall Esthetic Assessment Of Anterior Single-Tooth Implant Restoration: Modified Objective Criteria, Pongsakorn Komutpol

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The aim of this study was to observe the correlation of esthetic outcomes of single-tooth implant restoration when using Pink Esthetic Score/White Esthetic Score and the new modified assessment (Modified Objective Criteria; MOC) Materials and Methods: This crossectional retrospective study, up to 5 years, recruited 26 patients who single implant in maxillary central incisor area was placed. All the implants were recalled. Demographic data, intraoral photograph, dental model, periapical radiograph and CBCT were taken. One examiner assessed the esthetic outcomes by using PES/WES and MOC (gingiva, prosthesis and bone foundation) with 2 weeks between each evaluation. The score of …

The Longevity Of Ceramic Veneers: Clinical Evaluation Of Mechanical, Biological, And Esthetic Performances Of Ceramic Veneers Up To 7-Years Retrospective Study, Rapeepan Monaraks

Chulalongkorn University Theses and Dissertations (Chula ETD)

Purpose: To evaluate the longevity and mechanical, biological and aesthetic performance of ceramic veneers placed after 5 to 7 years in service. Materials and Methods: Ceramic veneer restorations were clinically examined following modified from the United States Public Health Service (USPHS) and World Dental Federation (FDI) criteria. Clinical evaluation was performed by two clinicians regarding mechanical, biological and aesthetic performances during maintenance appointments between September 2016 and August 2017. Intra-examiner and inter-examiner reliability were evaluated by calculating the intraclass correlation coefficient and inter-examiner calibration. The results were analysed by Kaplan-Meier survival estimation method and log-rank test at a 95% confidence …

A Comparison Of Taper-Implant Designs And Bone Quality On The Primary Stability: An In Vitro Biomechanical Study, Ratchaya Chayangsu

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective To investigate the effect of the taper-implant design and the effect of bone quality on the primary stability in terms of insertion torque test, removal torque test and resonance frequency analysis. Methods Five taper-implant designs were test in artificial bone blocks with four qualities. Five repetitions per implant design were placed in each bone quality started from softest bone block. The implant motor was used to prepared the osteotomy sites and implant insertion according to manufacturers' recommendation. Peak insertion torque values were measured and recorded by implant motor when the platform of the implant flush to the bone level. …

Host Responses In Peri Implant Tissue In Comparison To Periodontal Tissue, Raviporn Madarasmi

Chulalongkorn University Theses and Dissertations (Chula ETD)

The host response in peri-implant tissue may differ from that in periodontal tissue in a healthy individual. The purpose of this study is to investigate the expression of inflammatory cytokines in peri-implant crevicular fluid (PICF)from single implant with two different abutment types in comparison to healthy periodontal tissue. Moreover, the effect of these inflammatory cytokines on the expression of FAK and RANKL were also studied. Nineteen participants with healthy implants and teeth were recruited according to the inclusion and exclusion criteria. PICF and gingival crevicular fluid (GCF) was collected using sterile paper points. The expression level of inflammatory cytokines including …

Evaluation Of Voids In Class Ii Restorations Restored With Bulk-Fill And Conventional Nanohybrid Resin Composite, Saiisara Chaidarun

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: The aim of the study was to evaluate the influence of four resin composites on voids in small and large Class II cavities. Furthermore, the thickness of the first increment of the restorations was studied. Methods: Eighty artificial lower second premolars were divided into two preparation designs with 40 standardized Class II cavities in each, and then restored with four resin composites (three bulk-fill types: SonicFill 2, Filtek Bulk Fill (capsule), Filtek Bulk Fill (syringe) and a conventional nanohybrid resin composite: Premise). Restorations were sectioned for microscopic evaluation and a Kruskal-Wallis analysis was performed to evaluate the number of …

Immunohistochemical Assessment Of The Peri-Implant Soft Tissues Around Different Abutment Materials : An Experimental Study In Human, Sirikarn Thongmeearkom

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective To evaluate the effect of 4 different types of abutment material, which are titanium, zirconium oxide, gold alloy, and zirconia-coping cemented on titanium-base, on the surrounding soft tissues. Material and Methods Twenty dental implants in posterior edentulous area were randomly divided into 4 groups and inserted 4 types of abutment materials; Titanium, zirconia, gold-alloy, and titanium-base, on the implant installation surgery day. Eight weeks after implant surgery, peri-implant soft tissues around experimental abutments were harvested and split according to implant side; buccal, lingual, mesial, and distal. The specimens were processed through immunohistochemical preparation and stained with CD3, CD20, CD68, …

Prevalence Of Chronic Temporomandibular Disorder Pain And Comorbidities In A Group Of Thai Patient: A Cross Sectional Study, Tra Thu Nguyen

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: 1. to investigate the prevalence of chronic temporomandibular disorder (TMD) and its comorbidities in a group of Thai patients; 2. to evaluate the relationship between pain parameters (pain duration, pain intensity, and pain frequency) with the number of comorbidities and widespread pain index (WPI); 3. to assess the relationship between the presence of chronic pain with familial history in TMD pain patients. Methods: A cross-sectional study was undertaken of 351 outpatients. Patients were given an interviewer-administered questionnaire which contained four parts (demographic data, pain parameters, WPI and comorbidities diagnosis). Diagnosis was made in accordance with Diagnostic Criteria for Temporomandibular …

Patient's Perception And Satisfaction On Dental Implant Therapy By Postgraduate Dental Students And Experienced Dentists, Pat Vipattanaporn

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objective: To evaluate and compare patient's perception and satisfaction between patients who received dental treatment from postgraduate dental students and experienced dentists. Material and methods: This descriptive cross-sectional study was performed in patients who received the dental implant from Faculty of dentistry Chulalongkorn University. A data collection of patient's perception and satisfaction was done by questionnaire which was sent by mail. The questionnaire contained 23 statements including 7 statements of demographic data, 7 statements of perception, and 9 statements of satisfaction. The patient had to answer through the one-best-answer multiple choices and visual analog scale (VAS). Results: The 382 participants …

Comparison Of The Accuracy Of Surgical Guided Template Produced From Intraoral Scan Technique And Model Scan Technique For Implantation Using Computer Assisted Implant Surgery, Prudsaporn Kiatkroekkrai

Chulalongkorn University Theses and Dissertations (Chula ETD)

Objectives: To compare the accuracy of implant position placed by CAIS technique using surgical guided template produced by intraoral scan and model scan. Materials and methods: Patients who needed single tooth implant restoration were enrolled in this study and devided into two groups randomly according to the surface scan technique (n=30 each group). Intraoral scans were done using the Trios Scanner (3shape). While, diagnostic casts were scanned by D900L lab scanner (3shape). The templates were design by the clinician using coDiagnostiX software (Dental Wings Inc.). Implant surgery were performed according to fully CAIS protocol. CoDiagnostiX software was used to compare …