Pharmacology Commons^™

Effects Of Dry Binders On The Mechanical And Disintegration Properties Of Nava-Kote Tablets, Chaivut Lorpongpaiboon, Thitianan Kulsirirat, Kanagam Hammaworachat, Siracha Kasemsuwan, Sunee Channarong

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Development Of Dietary Supplement Tablets Containing The Extract Of Mangosteen Fruit Rind, Methanee Khoprasert, Pennapa Srichaiyon, Cholthicha Insom, Wiriyapom Sirikul, Werayut Pothitirat, Wandee Gritsanapan, Orawan Chitvanich

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Formulation Development Of Multilayer Sustained Release Tablets Of Sodium Valproate Combined With Valproic Acid, Worawan Saingam, Poj Kulvanich

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Quantitative Analysis Of “Tee Ka Tuk” Formulation Using Tlc-Densitometric Method, Maneekran Napakraw, Thitima Lhinhatrakool, Prasan Tanguenyongwatana

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Author Index, N/A

The Thai Journal of Pharmaceutical Sciences

No abstract provided.

Floating And Mucoadhesive System Of Furosemide: Development And In Vitro Evaluation, Krunal Takarkhede, Dilesh Jagdish, Shagufta Khan, Pramod Yeole

The Thai Journal of Pharmaceutical Sciences

Furosemide is poorly soluble in acidic pH and absorbed from the upper part of the Gl tract. We, therefore, aimed to develop a sustained release gastroretentive beads containing furosemide inclusion complex. The different formulations of floating beads we...

Influence Of Ephx1 Polymorphism And Clinical Factors On Carbamazepine Resistant Epilepsy, Khanistha Tuanthaisong, Yotin Chinvarun, Mayuree H. Tantisira, Pornpimol Kijsanayotin

The Thai Journal of Pharmaceutical Sciences

The treatment outcomes of carbamazepine (CBZ) vary widely among patients. Single nucleotide polymorphisms (SNPs) in genes involved in pharmacokinetics and pharmacodynamics of CBZ may influence patients’ response to CBZ. We aimed to investigate the asso...

Simultaneous Spectrophotometric Determination Of Difloxacin And Enrofloxacin In Urine Samples By Partial Least Squares Regression (Pls) And Direct Orthogonal Signal Correction-Partial Least Squares Regression (Disc-Ols) Methods, Kuldeep Kaur, Ashok Kumar Malik, Baldev Singh, M. Godarzi

The Thai Journal of Pharmaceutical Sciences

Simultaneous determination of difloxacin and enrofloxacin using two chemometric methods, partial least squares regression (PLS) and direct orthogonal signal correction-partial least squares regression (DOSC-PLS) is described in this paper. The simultaneous determination of these drugs is difficult due to spectral interferences. The calibration graphs were linear in the range of 1-10 μg mL-1 and 1-9 μg mL-1 with detection limits of 0.52 and 0.15 μg mL-1 for difloxacin and enrofloxacin, respectively. The experimental calibration matrix was designed with 25 mixtures of these drugs. The PLS and DOSC-PLS were used for multivariate calibration of spectrophotometric data. The root mean square error …

Facile Derivative Uv Spectroscopy Method: Simultaneous Estimation Of Tinidazole And Fluconazole In Combined Tablet Dosage Form, Saurabh Pandey, Preeti Pandey, Shaifali Dubey, Udisha Chaturvedi, Awani K. Rai

The Thai Journal of Pharmaceutical Sciences

Two derivative spectrophotometric methods have been developed for simultaneous determination of tinidazole and fluconazole in pharmaceutical formulations. The first method depends on utilization of first derivative UV spectrophotometry, with zero-crossing and peak-to-base measurement at 260.57 & 264.23 nm for fluconazole and 263.86 & 318.85 nm for tinidazole. The second method, compensation technique depends on first derivative of the ratio-spectra by measurements of the amplitudes for tinidazole and fluconazole. Calibration graphs were established for tinidazole and fluconazole in the range of 10-100 μg ml-1 and 2-20 μg ml-1, respectively. All proposed methods have been extensively validated. The results were found to …

Simultaneous Estimation Of Cefixime And Cloxacillin In Human Plasma By Reversed Phase-Hplc With Uv Detection, Sommath D. Bhinge, Sharangouda M. Malipatil, Lalit V. Sonawane

The Thai Journal of Pharmaceutical Sciences

An accurate, rapid and simple reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of cefixime, cloxacillin and for chloramphenicol (internal standard) in human plasma. Chromatographic separation was accomplished using CAPCELL PAK C8 (4.6 mm Ó 250 mm, 5 μm) analytical column using phosphate buffer, methanol and acetonitrile (40:45:15) as eluent. Detection and quantification were performed by UV/Vis detection at 225 nm. The lower limit of detection and quantification were 0.01 μg†mL-1 and 0.25 μg†mL-1 for cefixime, 0.03 μg†mL-1 and 0.39 μg†mL-1 for cloxacillin. The calibration curves were linear over the concentration range 0.5 to …

Thrombolytic Activity Of Some Spices And Plants Available In Bangladesh, Md. Rakib Ai-Manun, Nabiha Amrin, Jahura Begum, Md. Abdul Mazid

The Thai Journal of Pharmaceutical Sciences

Thrombolytic activities of some plants, namely Tamarindus indica (Fabaceae), Flemingia congesta (Fabaceae), Lawsonia inermis (Lythraceae), Mesua nagassarium (Clusiaceae, and spices, namely Coriandrum sativum (Apiaceae), Curcuma longa (Zingiberaceae), Cinnamomum tamala (Lauraceae), Nigella sativa (Ranunculaceae), Eugenia aromaticum (Myrtaceae), available in Bangladesh, were evaluated using an in vitro model. The thrombolytic activity in terms of percentage of weight loss of in vitro formed clots were found as C. sativum 43.25 ± 7.18%, C. longa 53.32 ± 4.96%, C. tamala 22.10 ± 3.18%, N. sativa 28.49 ± 3.72%, E. aromaticum 32.18 ± 3.10%, T. indica 28.91 ± 2.29%, F. congesta 35.27 ± 7.35%, L. …

Lactobacillus Plantarum T23/3 As A Potential Probiotic Strain Isolated From Faces, Titpawan Nakpheng, Rosainee Kha, Neelam Balekar, Sanae Kaewnopparat, Teerapol Srichana

The Thai Journal of Pharmaceutical Sciences

The present study was undertaken to evaluate the properties of Lactobacillus plantarum T23/3 isolated from the feces of healthy elderly volunteer. The L. plantarum T23/3 showed better tolerance property to bile acid, simulated gastric fluid (SGF) and simulated intestinal fluid (SIF), and exhibited antimicrobial activity against tested enteropathogens. It formed macroscopic granules with surface hydrophobicity and co-aggregated with tested microbial strain. It was resistant to some antibiotics used for treatment of diarrhea so there is possibility to co-administer L. plantarum as probiotic with antidiarrheal therapy. Furthermore, adhesion and significant potential for decreasing adhesion of pathogens (E. coli 2601 and V. …

Application Of A Stability-Indicating Hptlc Method For Simultaneous Determination Of Chloramphenicol And Dexamethasone Sodium Phosphate In Eye Drop, Yimer Seid, Araya Hymete, Adnan A. Bekhit

The Thai Journal of Pharmaceutical Sciences

A high performance thin layer chromatography (HPTLC) method was developed and validated for the simultaneous quantification of dexamethasone sodium phosphate and chloramphenicol in eye drop. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using acetonitrile: water: ammonia (10:3:0.5 v/v/v), pH adjusted to 7.8 with 1M HCl, as a mobile phase. The densitometric determination of dexamethasone phosphate and chloramphenicol was carried out at 262 nm. Calibration curves for dexamethasone phosphate and chloramphenicol were linear in the range of 80-300 ng and 400-1400 ng, respectively. The method was validated according to International Conference on Harmonization (ICH) guidelines …

Enhancement Of Wttability And In Vitro Dissoution Properties Of Lercanidipine Hydrochloride By Solid Dispersion Technique, Suneel Pandey, Ekunkumar Chandekar, Amarnath Wattamwar, Sachin Pandit, Hemant Joshee, Arun Patil

The Thai Journal of Pharmaceutical Sciences

The aim of the present study was to improve lercanidipine HCl solubility and in vitro dissolution rate by preparing solid dispersion with polyethylene glycol (PEG) 6000 using the solvent evaporation technique. Solid dispersions with PEG 6000 (as carriers) were prepared in drug: carrier (1:1, 1:5 and 1:10) ratios along with the corresponding physical mixtures. Analytical techniques, FT-IR spectroscopy, differential scanning calorimetry (DSC) and X-ray diffraction (XRD) were used to characterize the drug in the physical mixtures and solid dispersions. The solubility and wettability studies of solid dispersions as well as physical mixtures showed greater improvement compared to the pure drug. …

Cationic Amphiphilic Drug-Induced Autophagosome Accumulation Is Due To Autophagosome Sequestration Within Vimentin Intermediate Filament Networks Resulting In Prolonged Autophagosome Half-Life, Miriam Devorah Kleinman

Wayne State University Dissertations

Accumulations of autophagosomes and non-esterified cholesterol are

observed in several cell lines derived from lysosomal storage diseases,

including Niemann Pick Type C (NPC). The relationship between

autophagosome accumulation and lysosomal non-esterified cholesterol is

unclear. Exposure of murine hepatoma 1c1c7 cultures to the cationic

amphiphilic drugs (CADs) U18666A, imipramine and clozapine caused

lysosomal non-esterified cholesterol and autophagosome accumulation.

Measurement of LC3-II conversion in the presence of lysosomal inhibitors

bafilomycin A1 and NH4Cl, degradation of long-lived proteins, and

colocalization of GFP-LC3 and LAMP1 indicated an increase in

autophagosome synthesis without compensatory increase in clearance.

Autophagosome synthesis was blocked using 3-MA to monitor …

Effects Of Partner Proteins On Bca2 Ring Ligase Activity, Stephanie Bacopulos, Yutaka Amemiya, Wenyi Yang, Judit Zubovits, Angelika Burger, Martin Yaffe, Arun K. Seth

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

BCA2 is an E3 ligase linked with hormone responsive breast cancers. We have demonstrated previously that the RING E3 ligase BCA2 has autoubiquitination activity and is a very unstable protein. Previously, only Rab7, tetherin, ubiquitin and UBC9 were known to directly interact with BCA2.

Methods

Here, additional BCA2 binding proteins were found using yeast two-hybrid and bacterial-II-hybrid screening techniques with Human breast and HeLa cDNA libraries. Co-expression of these proteins was analyzed through IHC of TMAs. Investigation of the molecular interactions and effects were examined through a series of in vivo and in vitro assays.

Results

Ten unique …

Vascular Kv7 (Kcnq) Potassium Channels As Therapeutic Targets In Cerebral Vasospasm, Bharath Kumar Mani

Dissertations (6 month embargo)

Cerebral vasospasm, a grave sequel to subarachnoid hemorrhage (SAH), is characterized by prolonged severe constriction of arteries in the base of the brain, including the basilar artery. Spasmogens (serotonin, endothelin and vasopressin), elevated in response to SAH, induce persistent depolarization of the myocytes in the artery wall, leading to continuous influx of calcium (Ca2+) through voltage-sensitive Ca2+ channels (VSCC) resulting in hyperconstriction (spasm). The spasm of the arteries restricts blood flow to the brain, inducing ischemic neurological deficits and consequential high morbidity and mortality. The cellular pathogenesis of cerebral vasospasm is poorly understood and the therapeutic options are limited.

Kv7 …

Targeting Α4 Integrin Containing Complexes In Multiple Myeloma Using Peptidomimetics, Michael Foster Emmons

USF Tampa Graduate Theses and Dissertations

In our previous work we demonstrated that the integrin antagonist, HYD1, induced necrotic cell death in myeloma cell lines in vitro and in vivo as a single agent. In order to further delineate biomarkers of response to HYD1 we developed an isogenic drug resistant variant named H929-60. We show that the acquisition of resistance towards HYD1 correlates with reduced expression of the cleaved α4 integrin subunit and beta 1 integrin. Moreover, we demonstrate that HYD1 interacts with α4 integrin in myeloma cells. Consistent with reduced VLA-4 expression, the resistant variant showed ablated functional binding to fibronectin, VCAM-1 and the bone …

Use And Development Of Computational Tools In Drug Discovery: From Small Molecules To Cyclic Peptides, Daniel Navarrete Santiago

USF Tampa Graduate Theses and Dissertations

The scope of this work focuses on computationally modeling compounds with protein structures. While the impetus of drug discovery is the innovation of new therapeutic molecules, it also involves distinguishing molecules that would not be an effective drug. This can be achieved by inventing new tools or by refining old tools. Virtual screening (VS, also called docking), the computational modeling of a molecule in a receptor structure, is a staple in predicting a molecule's affinity for an intended target. In our Virtual Target Screening system (also called inverse-docking), VS is used to find high-affinity targets, which can potentially explain absorption, …

Skin Irritation Study Of Two Patented Mix Extracts From Emblica, Ma-Khwaen And Khanun, Sareeya Reungpatthanaphong, Tuanta Sematong, Buppachart Potduang, Phanukit Kunhachan, Cholticha Niwaspragrit

The Thai Journal of Pharmaceutical Sciences

No abstract provided.