Characterizing A Novel Monoclonal Ampa Receptor 1/2/3 Antibody In The Hippocampus And Prefrontal Cortex Of Rat, Monkey, And Human,
2014
Pitzer College
Characterizing A Novel Monoclonal Ampa Receptor 1/2/3 Antibody In The Hippocampus And Prefrontal Cortex Of Rat, Monkey, And Human, Sebastian Aguiar
Pitzer Senior Theses
The excitatory, ionotropic glutamatergic AMPA receptor is the most common membrane-bound receptor in the central nervous system. AMPARs and the NMDA receptors are central to synaptic plasticity, memory, and mechanisms of neurodegeneration. The AMPAR is an obligate heterotetramer, composed of subunits GluA1-4. Subunit permutation determines ion conductance, trafficking and other functional characteristics. Few available antibodies are subunit-specific, disabling researchers from accurately visualizing differential AMPAR subunit distribution in the nervous system. This study sought to visualize a novel monoclonal GluA1/2/3 antibody with functional avidity for three of four receptor subunits and to characterize the ultrastructural localization of these receptors using confocal …
Mechanisms Of Cyclooxygenase-2-Dependent Human Aortic Smooth Muscle Cell Phenotypic Modulation,
2014
University of Kentucky
Mechanisms Of Cyclooxygenase-2-Dependent Human Aortic Smooth Muscle Cell Phenotypic Modulation, Oreoluwa O. Adedoyin
Theses and Dissertations--Pharmacy
Abdominal aortic aneurysm (AAA) is a disease of the aorta characterized by pathological remodeling and progressive weakening of the vessel resulting in the increased risk of rupture and sudden death. In a mouse model of the disease induced by chronic Angiotensin II (AngII) infusion, progression of AAAs is associated with reduced differentiation of smooth muscle cells (SMCs) at the site of lesion development. In the mouse model, the effectiveness of cyclooxygenase-2 (COX-2) inhibition for attenuating AAA progression is associated with maintenance of a differentiated SMC phenotype. However, the safety of COX-2 inhibitors is currently in question due to the increased …
Flavonoids With Novel Nicotinic Activity As Potential Pharmacotherapies To Treat Ethanol-Induced Neurotoxicity,
2014
University of Kentucky
Flavonoids With Novel Nicotinic Activity As Potential Pharmacotherapies To Treat Ethanol-Induced Neurotoxicity, Joseph A. Lutz
Theses and Dissertations--Pharmacy
Ethanol causes neurotoxicity via several mechanisms at different points in the cycle of dependence, including neuroinflammation and oxidative stress during ethanol exposure as well as excitotoxicity during ethanol withdrawal. The primary therapeutic implication is that ethanol-induced neurotoxicity requires multifunctional pharmacotherapies which reduce all mechanisms. Using an innovative pharmacological high throughput screening method on a large plant extract library we discovered flavonoids with alpha7 nicotinic acetylcholine receptor (nAChR) activity. In addition to their well-known anti-inflammatory and antioxidant properties, this novel activity means they can potentially reduce excitotoxicity and therefore makes them ideal for inhibition of ethanol-induced neurotoxicity. Rhamnetin, the candidate compound, …
Comparison Of Eight Published Models For Estimating Vancomycin Clearance From Creatinine Clearance In Thai Adult Patients,
2014
Chulalongkorn University
Comparison Of Eight Published Models For Estimating Vancomycin Clearance From Creatinine Clearance In Thai Adult Patients, Aim-On Pradoo, Thitima Wattanavijitkul, Darunee Chotiprasitsakul, Duangchit Panomvana
The Thai Journal of Pharmaceutical Sciences
This prospective study was performed to determine the best published model for estimating vancomycin clearance from creatinine clearance (CLcr) in Thai adult patients with CLcr greater than or equal to 30 ml/min. Data from 36 adult patients, who were giv...
Molecular Mechanisms Of Chemotherapeutic Resistance In Lung Cancer,
2014
Chulalongkorn University
Molecular Mechanisms Of Chemotherapeutic Resistance In Lung Cancer, Piyaparisorn Wongvaranon, Varisa Pongrakhananon, Pithi Chanvorachote
The Thai Journal of Pharmaceutical Sciences
Lung cancer mortality is mainly due to the high rate of chemotherapeutic resistance frequently found in lung cancer patients. In order to develop the novel strategies for effective treatment, the molecular basis regarding adaptive machineries of lung can...
Understanding The Medication Experiences Of Thai Patients Attending A Medication Therapy Management Clinic,
2014
Chulalongkorn University
Understanding The Medication Experiences Of Thai Patients Attending A Medication Therapy Management Clinic, Phantipa Sakthong, Win Winit-Watjana, Rungpetch Sakulbumrungsil
The Thai Journal of Pharmaceutical Sciences
The study aimed to explore Thai patients' medication experiences in five domains, i.e., attitudes, understanding, expectations and wants, concerns, and convenience of medicine use. This cross-sectional descriptive study was carried out using a qualitativ...
Identification And Antimicrobial Activity Of Streptomyces Strains From Thai Mangrove Sediment,
2014
Chulalongkorn University
Identification And Antimicrobial Activity Of Streptomyces Strains From Thai Mangrove Sediment, Wongsakorn Phongsopitanun, Khanit Suwanborirux, Somboon Tanasupawat
The Thai Journal of Pharmaceutical Sciences
Two actinomycete strains, D2-1 and D2-2, were isolated from mangrove sediment sample collected from Samut Song Kram province, Thailand. On the screening of antimicrobial activity, the crude extracts of fermentation broth of both strains exhibited activit...
Gigantol-Induced Apoptosis In Lung Cancer Cell Through Mitochondrial-Dependent Pathway,
2014
Chulalongkorn University
Gigantol-Induced Apoptosis In Lung Cancer Cell Through Mitochondrial-Dependent Pathway, Sopanya Charoenrungruang, Pithi Chanvorachote, Boonchoo Sritularak, Varisa Pongrakhananon
The Thai Journal of Pharmaceutical Sciences
Lung cancer is identified as a major cause of cancer-related death. Death in this cancer associates with the acquisition of apoptosis avoidance mechanism. Apoptosis, a programmed cell death mechanism, plays an important role on normal cell development an...
Balancing Accountability For Adverse Drug Reactions Under American Product Liability Law: Lessons For Thailand?,
2014
Chulalongkorn University
Balancing Accountability For Adverse Drug Reactions Under American Product Liability Law: Lessons For Thailand?, Sakda Thanitcul, Matthew Lim Braslow
The Thai Journal of Pharmaceutical Sciences
Drugs that are powerful enough to cure disease are powerful enough to do potential harm. This common saying among physicians is the central point of this paper. When harm to a patient does occur, the sense of society requires that where and when appropri...
The Efficacy Of Prostaglandin Synthase Inhibition On Attenuating Pressure Overload Induced Cardiac Remodeling,
2014
University of Mississippi
The Efficacy Of Prostaglandin Synthase Inhibition On Attenuating Pressure Overload Induced Cardiac Remodeling, Krishna T. Nagalla
Electronic Theses and Dissertations
Chronic hypertension has long been known to cause left ventricular remodeling. Although previous studies pointed towards inflammation as the pathological driver of cardiac remodeling, the exact mechanistic pathway associated with pressure overload-induced cardiac remodeling remains to be elucidated. In order to address this issue, we designed this study to determine pathways associated with pressure overload induced cardiac remodeling and identify therapeutic targets to attenuate this maladaptive cardiac remodeling. Rats with surgically constricted abdominal aorta were used as an animal model for pressure overload and associated Left ventricular (LV) structure and functional changes were measured by pressure-volume admittance catheterization. Human cardiac …
Adenosine A2b Receptor Effects On Post-Mi Remodeling And Cardiac Fibroblast Function,
2014
Wayne State University
Adenosine A2b Receptor Effects On Post-Mi Remodeling And Cardiac Fibroblast Function, Enbo Zhan
Wayne State University Dissertations
Adenosine A2B receptor (A2BR) appear to contribute to chronic inflammation. This receptor is highly expressed in macrophages and cardiac fibroblasts, cells which play key roles in inflammation and healing following myocardial infarction (MI). A2BR have been shown to induce collagen production and promote organ fibrosis, although the reports of A2BR role on MI are limited and conflicting. The results of cardiac fibroblast (CF) studies however suggest that non-selective A2BR stimulation inhibits collagen expression. The hypothesis of the present study was that deletion of A2BR reduces adverse remodeling in post-MI, …
Protective Effect Of Deferiprone On 7-Ketocholesterol Formation In Hemin-Induced Ldl Oxidation,
2014
Chulalongkorn University
Protective Effect Of Deferiprone On 7-Ketocholesterol Formation In Hemin-Induced Ldl Oxidation, Usa Suwannasual, Thitima Pengsuparp, Rataya Luechapudiporn
The Thai Journal of Pharmaceutical Sciences
Hemin (iron (III)-protoporphyrin IX) is found that it is elevated in the plasma of thalassemic patients and is a possible cause of oxidative stress-promoting atherosclerosis. Deferiprone (L1), an iron chelator, can prevent free radical formation catalyze...
Development And Validation Of Rp-Hplc Method For Analysis And Stability Study Of 8-Carboxyphenyl-2'-Deoxyguanosine And 8-Bromo-2'-Deoxyguanosine,
2014
Chulalongkorn University
Development And Validation Of Rp-Hplc Method For Analysis And Stability Study Of 8-Carboxyphenyl-2'-Deoxyguanosine And 8-Bromo-2'-Deoxyguanosine, Kulwadee Sawatpaiboontawee, Panutsaya Sermthaveesub, Panuwat Sangsirimongkolying, Patratorn Kunakorntham, Sudarat Thongborriboon, Bodin Tuesuwan, Vorasit Vongsutilers
The Thai Journal of Pharmaceutical Sciences
Modifications on C8 position of purine base have been studied for their effects on B-DNA to Z-DNA conversion. The 8-bromo and 8-carboxyphenyl adduct are among the most potent Z-DNA facil tators and hence arc aimed to be utilized for Z-DNA based probe lor...
Comparative Study Of Postmortem Blood, Urine And Vitreous Humor Methamphetamine,
2014
Chulalongkorn University
Comparative Study Of Postmortem Blood, Urine And Vitreous Humor Methamphetamine, Rungtip Narapanyakul, Wichian Tungtananuwat, Patramon Yongpanich, Theerin Sinchai, Nantana Thong-Ra-Ar, Somsong Lawanprasert
The Thai Journal of Pharmaceutical Sciences
Methamphetamine (MA) is a highly addictive and toxic central nervous system stimulant. Urine samples are used as evidences of MA abuse under the Narcotics Laws, whereas blood samples are specimens for investigation of MA poisoning. However, both specimen...
Evaluation Of Antioxidant And Free Radical Scavenging Activities Of Different Fractions Of Pterospermum Suberifolium Leaf Extract,
2014
Chulalongkorn University
Evaluation Of Antioxidant And Free Radical Scavenging Activities Of Different Fractions Of Pterospermum Suberifolium Leaf Extract, Dibyajyoti Saha, Swati Paul
The Thai Journal of Pharmaceutical Sciences
The study was aimed an evaluating the antioxidant activities of the various leaf extracts (ethyl acetate, n- butanol and chloroform) of Pterospermum suberifolium by using various in vitro antioxidant assays. The total polyphenols were also determined spe...
Determination Of Retinol And Alpha-Tocopherol In Avocado Nanoemulsion With Monolithic Column,
2014
Chulalongkorn University
Determination Of Retinol And Alpha-Tocopherol In Avocado Nanoemulsion With Monolithic Column, Wisanu Thongchai
The Thai Journal of Pharmaceutical Sciences
A high performance liquid chromatographic procedure was developed for separation of retinol and a-tocopherol from avocado oil. The extraction methods were studied to extract of oil from avocado sample. This method was developed and validated for various ...
A New Rp-Hplc Method For Estimation Of Clindamycin And Adapalene In Gel Formulation: Development And Validation Consideration,
2014
Chulalongkorn University
A New Rp-Hplc Method For Estimation Of Clindamycin And Adapalene In Gel Formulation: Development And Validation Consideration, Rohit. H. Khatri, Rashmin. B. Patel, Mrunali R. Patel
The Thai Journal of Pharmaceutical Sciences
This paper describes a validated high-performance liquid chromatographic (HPLC) method for simultaneous estimation of clindamycin (CLI) and adapalene (ADA) in pure powder and gel formulation. The HPLC separation was achieved on a Luna C18 (2) (250 x 4.6 ...
Verification Of Using Saliva As An Alternative To Serum For Topiramate Monitoring,
2014
Chulalongkorn University
Verification Of Using Saliva As An Alternative To Serum For Topiramate Monitoring, Jareerut Kongrit, Yotin Chinvarun, Nuansri Niwattisaiwong, Somsong Lawanprasert
The Thai Journal of Pharmaceutical Sciences
This study aims to verify the correlation between serum and saliva topiramate concentrations in Thai patients with epilepsy. Twenty-six subjects were patients aged 17-56 years old who were under treatment with topiramate. Their blood and saliva samples w...
Authentication Of A Thai Crude Drug "Kot-Chula-Lumpa" Using Gas Chromatography-Mass Spectrometry And Multivariate Data Analysis,
2014
Chulalongkorn University
Authentication Of A Thai Crude Drug "Kot-Chula-Lumpa" Using Gas Chromatography-Mass Spectrometry And Multivariate Data Analysis, Jankana Burana-Osot, Panadda Phattanawasin, Sununta Srisopon, Praveen Kumar Ashok, Baokang Huang, Uthai Sotanaphun
The Thai Journal of Pharmaceutical Sciences
Kot-chula-lumpa is a crude drug of Thai traditional medicine for fever remedy. To authenticate its botanical origin, gas chromatogram of its non-polar constituents was analysed and compared with those of authentic samples by using chemometric tools. Fing...
Association Of Genetic Variants In Ugt1a6 Genes And Non-Genetic Variant With Valproic Acid Doses And Plasma Concentration In Thai Epileptic Patients,
2014
Chulalongkorn University
Association Of Genetic Variants In Ugt1a6 Genes And Non-Genetic Variant With Valproic Acid Doses And Plasma Concentration In Thai Epileptic Patients, Salinthip Aphichartphunkawee, Yotin Chinvarun, Pornpimol Kijsanayotin
The Thai Journal of Pharmaceutical Sciences
The aim of this study was to investigate the association of genetic variants in UGT1A6 19T>G, 541A>G and 552A>C together with non-genetic variant with VPA dose and plasma concentrations in Thai epileptic patients. Eighty four Thai epileptic patients who ...
