A Rapid Lc-Ms/Ms Method For Simultaneous Determination Of Quetiapine And Duloxetine In Rat Plasma And Its Application To Pharmacokinetic Interaction Study,
2020
Taiwan Food and Drug Administration
A Rapid Lc-Ms/Ms Method For Simultaneous Determination Of Quetiapine And Duloxetine In Rat Plasma And Its Application To Pharmacokinetic Interaction Study, X. Chen, C. Liang, L. Cui, J. Le, Z. Qian, R. Zhang, Z. Hong, Y. Chai
Journal of Food and Drug Analysis
Combinations of new antidepressants like duloxetine and second-generation antipsychotics like quetiapine are used in clinical treatment of major depressive disorder, as well as in forensic toxicology scenarios. The drug–drug interaction (DDI) between quetiapine and duloxetine is worthy of attention to avoid unnecessary adverse effects. However, no pharmacokinetic DDI studies of quetiapine and duloxetine have been reported. In the present study, a rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of quetiapine and duloxetine in rat plasma. A one-step protein precipitation with acetonitrile was applied for sample preparation. The analytes were eluted on an …
Abelmoschus Esculentus Subfractions Improved Nephropathy With Regulating Dipeptidyl Peptidase-4 And Type 1 Glucagon-Like Peptide Receptor In Type 2 Diabetic Rats,
2020
Taiwan Food and Drug Administration
Abelmoschus Esculentus Subfractions Improved Nephropathy With Regulating Dipeptidyl Peptidase-4 And Type 1 Glucagon-Like Peptide Receptor In Type 2 Diabetic Rats, C.-H. Peng, H.-C. Lin, C.-L. Lin, C.-J. Wang, C.-N. Huang
Journal of Food and Drug Analysis
Abelmoschus esculentus (AE) has been used in traditional medicine to ameliorate hyperglycemia, but its mucilage increased bioassay difficulties. We have obtained a series of AE subfractions. Among them F1 and F2 regulated dipeptidyl peptidase-4 (DPP-4) and type 1 glucagon-like peptide receptor (GLP-1R), the treatment targets for type 2 diabetes. F1, F2 and fraction residues (FR) showed advantage on different aspects, which attenuates insulin resistance and metabolic disorder in vivo, and prevents renal-tubular change in vitro. In the present study, using type 2 diabetes model induced by high fat diet (HFD) and streptozotocin (STZ), we aim to investigate whether AE prevent …
Encapsulation Of Babchi Essential Oil Into Microsponges: Physicochemical Properties, Cytotoxic Evaluation And Anti-Microbial Activity,
2020
Taiwan Food and Drug Administration
Encapsulation Of Babchi Essential Oil Into Microsponges: Physicochemical Properties, Cytotoxic Evaluation And Anti-Microbial Activity, G. Wadhwa, S. Kumar, V. Mittal, R. Rao
Journal of Food and Drug Analysis
Babchi essential oil (BEO) is a valuable essential oil reported to possess a variety of biological activities such as antitumor, anti inflammatory, immunomodulatory, antioxidant, antifungal and antibacterial properties. Due to its anti-microbial properties, this oil possesses an immense potential for the treatment of dermatological disorders. Further, it has minimal tendency to develop resistance, a common issue with most of the antibiotics. However, its highly viscous nature and poor stability in the presence of light, air and high temperature, limits its practical applications. To surmount these issues, this research aims to encapsulate BEO in ethyl cellulose (EC) microsponges for enhanced stability, …
Rubiscolin-6 Activates Opioid Receptors To Enhance Glucose Uptake In Skeletal Muscle,
2020
Taiwan Food and Drug Administration
Rubiscolin-6 Activates Opioid Receptors To Enhance Glucose Uptake In Skeletal Muscle, T.S. Kairupan, K.-C. Cheng, A. Asakawa, H. Amitani, T. Yagi, K. Ataka, N.T. Rokot, N.H. Kapantow, I. Kato, A. Inui
Journal of Food and Drug Analysis
Rubiscolin-6 is an opioid peptide derived from plant ribulose bisphosphate carboxylase/oxygenase (Rubisco). It has been demonstrated that opioid receptors could control glucose homeostasis in skeletal muscle independent of insulin action. Therefore, Rubiscolin-6 may be involved in the control of glucose metabolism. In the present study, we investigated the effect of rubiscolin-6 on glucose uptake in skeletal muscle. Rubiscolin-6-induced glucose uptake was measured using the fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) in L6 and C2C12 cell lines. The protein expressions of glucose transporter 4 (GLUT4) and AMP-activated protein kinase (AMPK) in L6 cells were observed by Western blotting. The in vivo effects …
The Rapid And Sensitive Detection Of Edible Bird's Nest (Aerodramus Fuciphagus) In Processed Food By A Loop-Mediated Isothermal Amplification (Lamp) Assay,
2020
Taiwan Food and Drug Administration
The Rapid And Sensitive Detection Of Edible Bird's Nest (Aerodramus Fuciphagus) In Processed Food By A Loop-Mediated Isothermal Amplification (Lamp) Assay, M.-S. Lee, J.-Y. Huang, Y.-Y. Lien, S.-C. Sheu
Journal of Food and Drug Analysis
Edible bird's nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers’ interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. …
Ubiquitin-Protein Ligase E3a (Ube3a) As A New Biomarker Of Cardiac Hypertrophy In Cell Models,
2020
Taiwan Food and Drug Administration
Ubiquitin-Protein Ligase E3a (Ube3a) As A New Biomarker Of Cardiac Hypertrophy In Cell Models, K.-C. Cheng, Y. Li, W.-T. Chang, Z.-C. Chen, J.-T. Cheng, C.-C. Tsai
Journal of Food and Drug Analysis
Cardiac hypertrophy is widely diagnosed in clinical cardiac disorders. The pathophysiology of hypertrophy is complex and multifactorial, a series of molecular and cellular changes are participated, such as activation of different signaling pathways, a switch of fetal gene program in the myocardium, and apoptosis. Some biomarkers have been applied to assess cardiac hypertrophy including atrial natriuretic peptides (ANP), brain/B-type natriuretic peptides (BNP), and α- or β- Myosin Heavy Chain (MHC) in addition to others. Recently, ubiquitin-protein ligase E3A (UBE3A) has been observed to increase in cardiac hypertrophy. Therefore, UBE3A as a new biomarker seems valuable in the clinic. The cardiac …
Antidepressant And Anxiolytic Effects Of The Proprietary Chinese Medicine Shexiang Baoxin Pill In Mice With Chronic Unpredictable Mild Stress,
2020
Taiwan Food and Drug Administration
Antidepressant And Anxiolytic Effects Of The Proprietary Chinese Medicine Shexiang Baoxin Pill In Mice With Chronic Unpredictable Mild Stress, X.-D. Zhou, D.-D. Shi, Z.-J. Zhang
Journal of Food and Drug Analysis
Depression and anxiety often co-occur with cardiac diseases. The Shexiang Baoxin pill (SBP) is a proprietary Chinese medicine initially used to treat cardiac conditions. This study explored whether SBP has antidepressant and anxiolytic effects in addition to hormonal and psychotropic mechanisms. Mice underwent 6 weeks of chronic unpredictable mild stress (CUMS) to induce depression- and anxiety-like behavior. During the 6-week experiment, mice received SBP at intragastric doses of 20.25 mg/kg or 40.5 mg/kg daily. Animals were then tested for depression in sucrose preference, forced-swimming, and tail suspension paradigms, and for anxiety in open field and elevated plus maze tests. Both …
Novel Technologies In Detection, Treatment And Prevention Of Substance Use Disorders,
2020
Taiwan Food and Drug Administration
Novel Technologies In Detection, Treatment And Prevention Of Substance Use Disorders, H. Moulahoum, F. Zihnioglu, S. Timur, H. Coskunol
Journal of Food and Drug Analysis
Substance use disorders are a widely recognized problem, which affects various levels of communities and influenced the world socioeconomically. Its source is deeply embedded in the global population. In order to fight against such an adversary, governments have spared no efforts in implementing substance abuse treatment centers and funding research to develop treatments and prevention procedures. In this review, we will discuss the use of immunological-based treatments and detection kit technologies. We will be detailing the steps followed to produce performant antibodies (antigens, carriers, and adjuvants) focusing on cocaine and methamphetamine as examples. Furthermore, part of this review is dedicated …
Determination Of Sulfamonomethoxine In Tilapia (Oreochromis Niloticus × Oreochromis Mossambicus) By Liquid Chromatography-Tandem Mass Spectrometry And Its Application Pharmacokinetics Study,
2020
Taiwan Food and Drug Administration
Determination Of Sulfamonomethoxine In Tilapia (Oreochromis Niloticus × Oreochromis Mossambicus) By Liquid Chromatography-Tandem Mass Spectrometry And Its Application Pharmacokinetics Study, T.-A. Kung, S.-H. Lee, W.-H. Wang
Journal of Food and Drug Analysis
A precise and reliable analytical method to measure trace levels of sulfamonomethoxine (SMM) and N4-acetyl metabolite in tilapia samples using liquid chromatography-tandem mass spectrometry was developed. Optimized chromatographic separation was performed on C18 reversed-phase columns using gradient elution with methanol and 5 mmol/L of an ammonium acetate aqueous solution (adjusted to pH 3.5 using formic acid). This study investigated the pharmacokinetic properties and tissue distribution of SMM and its major metabolite N4-acetyl sulfamonomethoxine (AC-SMM) in tilapia after a single dose of 100 mg kg−1 body weight of orally administered SMM. Blood and tissues were collected between 0.5 and 192 h …
Identification Of Baicalin From Bofutsushosan And Daisaikoto As A Potent Inducer Of Glucose Uptake And Modulator Of Insulin Signaling-Associated Pathways,
2020
Taiwan Food and Drug Administration
Identification Of Baicalin From Bofutsushosan And Daisaikoto As A Potent Inducer Of Glucose Uptake And Modulator Of Insulin Signaling-Associated Pathways, Y.-T. Kuo, C.-C. Lin, H.-T. Kuo, J.-H. Hung, C.-H. Liu, A. Jassey, M.-H. Yen, S.-J. Wu, L.-T. Lin
Journal of Food and Drug Analysis
Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by hyperglycemia that can lead to long-term complications including heart diseases, stroke, retinopathy, and renal failure. Treatment strategies include stimulating glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated to improve metabolism-associated disorders including obesity, hyperlipidemia, and nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, we explored in this study the effect of BOF and DAI extracts on glucose absorption and used them as source to identify phytochemical stimulator of glucose absorption. Glucose uptake and mechanistic studies …
Rapid Screening Of Sulfonamides In Dietary Supplements Based On Extracted Common Ion Chromatogram And Neutral Loss Scan By Lc-Q/Tof-Mass Spectrometry,
2020
Taiwan Food and Drug Administration
Rapid Screening Of Sulfonamides In Dietary Supplements Based On Extracted Common Ion Chromatogram And Neutral Loss Scan By Lc-Q/Tof-Mass Spectrometry, N.-Y. Ki, J. Hur, B.H. Kim, K.H. Kim, B.J. Moon, H.B. Oh, J. Hong
Journal of Food and Drug Analysis
There is an increasing amount of dietary supplements that are adulterated with diuretics and anti-diabetic drugs; this has become a global problem due to the wide distribution of dietary supplements and the serious negative health effects of the adulterants. In this study, a rapid screening method was developed for detection and confirmation of 35 sulfonamides in supplements by ultra-high performance liquid chromatography quadrupole/time of flight mass spectrometry. For effective extraction of sulfonamides from dietary supplements, four extraction protocols including HLB and WAX solid-phase extraction, Quick Easy Cheap Effective Rugged and Safe method, and pH-controlled liquid–liquid extraction were evaluated, and pH-controlled …
In Vivo Pro-Angiogenic Effects Of Dracorhodin Perchlorate In Zebrafish Embryos: A Novel Bioactivity Evaluation Platform For Commercial Dragon Blood Samples,
2020
Taiwan Food and Drug Administration
In Vivo Pro-Angiogenic Effects Of Dracorhodin Perchlorate In Zebrafish Embryos: A Novel Bioactivity Evaluation Platform For Commercial Dragon Blood Samples, P. Krishnaraj, Y. Chang, T.-J. Ho, N.-C. Lu, M.-D. Lin, H.-P. Chen
Journal of Food and Drug Analysis
Dragon blood has been used in wound treatment for many years and can be obtained from several distinct plant species. Dracorhodin, the active substituent of dragon blood, is a characteristic compound of the palm tree, Daemonorops draco. At present, the only method to evaluate the quality of commercial dragon blood samples is a HPLC method which determines the amount of dracorhodin in a dragon blood sample. In this study, we used zebrafish embryos as a platform to demonstrate the in vivo pro-angiogenic activity of dracorhodin perchlorate, the chemically synthesized analog of dracorhodin. By using this platform, three different commercial dragon …
Applications Of Cyclic Peptide Nanotubes (Cpnts),
2020
Taiwan Food and Drug Administration
Applications Of Cyclic Peptide Nanotubes (Cpnts), W.-H. Hsieh, J. Liaw
Journal of Food and Drug Analysis
Self-assembled cyclic peptide nanotubes (cPNTs) have recently drawn particular attention as one of the most intriguing nanostructures in the field of nanotechnology. Given their unique features including high surface area, increased drug loading, environmental stability, enhanced permeation, and modifiable drug release, these hollow tubular structures can be constructed with cyclic di-, tri-, tetra-, hexa-, octa-, and decapeptides with various amino acid sequences, enantiomers, and functionalized side chains and can be applied for antiviral and antibacterial drugs, drug delivery and gene delivery vectors, organic electronic devices, and ionic or molecular channels. Recent publications have presented promising results regarding the use of …
Novel Inspection Of Sugar Residue And Origin In Honey Based On The 13c/12c Isotopic Ratio And Protein Content,
2020
Taiwan Food and Drug Administration
Novel Inspection Of Sugar Residue And Origin In Honey Based On The 13c/12c Isotopic Ratio And Protein Content, C.-T. Chen, B.-Y. Chen, Y.-S. Nai, Y.-M. Chang, K.-H. Chen, Y.-W. Chen
Journal of Food and Drug Analysis
Regarding the honey industry, market prices are strongly affected by the origin and composition of products. In particular, the adulteration of honey can be divided into cases of honey being mixed with artificial syrup, the different origin of the adulteration and the presence of cane sugar residue. Unfortunately, recent studies mentioned that most honey is mixed with artificial syrups. Thus, determining such unnaturally present sugar is necessary to maintain the trust of the consuming populations. To investigate the possibility of syrup augmentation, this study first clarifies two points of great importance. First, long-term feeding of cane sugar to honey bee …
Ccm111 Prevents Hepatic Fibrosis Via Cooperative Inhibition Of Tgf-Β Wnt And Stat3 Signaling Pathways,
2020
Taiwan Food and Drug Administration
Ccm111 Prevents Hepatic Fibrosis Via Cooperative Inhibition Of Tgf-Β Wnt And Stat3 Signaling Pathways, I.-Y. Lin, Y.-S. Chiou, L.-C. Wu, C.-Y. Tsai, C.-T. Chen, W.-C. Chuang, M.-C. Lee, C.-C. Lin, T.-T. Lin, S.-C. Chen, M.-H. Pan, N. Ma
Journal of Food and Drug Analysis
CCM111 is an aqueous extract of Antrodia cinnamomea (AC) that has exhibited anti-liver fibrosis functions. However, the detailed mechanisms of AC action against liver fibrosis have not been elucidated yet. The present research showed that CCM111 significantly lowered the levels of the hepatic enzyme markers glutamate oxaloacetate transaminase (GOT) and glutamic pyruvic transaminase (GPT), prevented liver damage and collagen deposition, and downregulated TGF-β/Smad signaling in a dose-dependent manner compared with CCl4 treatment alone. CCM111 markedly inhibited TGF-β Wnt and STAT3 signaling pathway-regulated downstream genes in the liver by next-generation sequencing. The antifibrotic mechanisms of CCM111 were further demonstrated in HSC-T6 …
Simultaneous Quantification Combined With Multivariate Statistical Analysis Of Multiple Chemical Markers Of Wu Ji Bai Feng Pill By Uhplc–Ms/Ms,
2020
Taiwan Food and Drug Administration
Simultaneous Quantification Combined With Multivariate Statistical Analysis Of Multiple Chemical Markers Of Wu Ji Bai Feng Pill By Uhplc–Ms/Ms, S.-N. Duan, W. Qi, S.-W. Zhang, K.-K. Huang, D. Yuan
Journal of Food and Drug Analysis
Wu Ji Bai Feng Pill (WJBFP) is a traditional Chinese medicine (TCM) complex formula, which has been widely used in the treatment of various gynecological disorders. However, the quality control of multiple components in WJBFP is challengeable by using the methods applicable to analysis of several phytochemicals in single herbs or simple herbal preparations. The purpose of this study is to establish an ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC–MS/MS) method for the quantitative determination of 20 bioactive compounds in WJBFP. The modified chromatographic conditions were achieved on an Agilent Poroshell 120 EC-C18 column with a …
Shenmai-Yin Decreased The Clearance Of Nifedipine In Rats: The Involvement Of Time-Dependent Inhibition Of Nifedipine Oxidation,
2020
Taiwan Food and Drug Administration
Shenmai-Yin Decreased The Clearance Of Nifedipine In Rats: The Involvement Of Time-Dependent Inhibition Of Nifedipine Oxidation, H.-J. Wang, C.-K. Lu, W.-C. Chen, A.-C. Chen, Y.-F. Ueng
Journal of Food and Drug Analysis
The traditional Chinese herbal formula Shenmai-Yin (SY) and nifedipine have both been used to treat patients with cardiovascular disorders. Nifedipine is primarily oxidized by cytochrome P450 (CYP) 3A. The oxidation and pharmacokinetics of nifedipine were studied in rats in vitro and in vivo to illustrate the interaction of SY with nifedipine. Schisandrol A, schisandrin A and schisandrin B were identified as the main lignans in SY. In the study in vitro, the ethanolic extract of SY was used due to the solubility and the extract inhibited nifedipine oxidation (NFO) activity in a time-dependent manner. Among lignans, schisandrin B caused the …
Comparative Investigation For Raw And Processed Aconiti Lateralis Radix Using Chemical Uplc-Ms Profiling And Multivariate Classification Techniques,
2020
Taiwan Food and Drug Administration
Comparative Investigation For Raw And Processed Aconiti Lateralis Radix Using Chemical Uplc-Ms Profiling And Multivariate Classification Techniques, L. Sun, G. You, X. Cao, M. Wang, X. Ren
Journal of Food and Drug Analysis
A strategy combining chemical UPLC-MS profiling and multivariate classification techniques has been used for the comparison of raw and processed Aconiti Lateralis Radix. UPLC-MS was used to identify 18 characteristic compounds, which were selected for discrimination of the raw and two processed products (Heishunpian and Baifupian). Chemometric analyses, including the combination of a heat map and hierarchical cluster analysis (HCA) and principal component analysis (PCA), were used to visualize the discrimination of raw and two processed products. HCA and PCA provided a clear discrimination of raw Aconiti Lateralis Radix, Heishunpian and Baifupian. Finally, the counter-propagation artificial neural network (CP-ANN) was …
The Regulation Of Drug-Metabolizing Enzymes And Membrane Transporters By Inflammation: Evidences In Inflammatory Diseases And Age-Related Disorders,
2020
Taiwan Food and Drug Administration
The Regulation Of Drug-Metabolizing Enzymes And Membrane Transporters By Inflammation: Evidences In Inflammatory Diseases And Age-Related Disorders, K.-C. Wu, C.-J. Lin
Journal of Food and Drug Analysis
Drug-metabolizing enzymes (DMEs) and membrane transporters play important roles in the absorption, distribution, metabolism, and excretion processes that determine the pharmacokinetics of drugs. Inflammation has been shown to regulate the expression and function of these drug-processing proteins. Given that inflammation is a common feature of many diseases, in this review, the general mechanisms for inflammation-mediated regulation of DMEs and transporters are described. Also, evidences regarding the aberrant expression of these drug-processing proteins in several inflammatory diseases and age-related disorders are provided. © 2018
Antcins, Triterpenoids From Antrodia Cinnamomea, As New Agonists For Peroxisome Proliferator-Activated Receptor Α,
2020
Taiwan Food and Drug Administration
Antcins, Triterpenoids From Antrodia Cinnamomea, As New Agonists For Peroxisome Proliferator-Activated Receptor Α, Y.-J. Wang, S.-C. Lee, C.-H. Hsu, Y.-H. Kuo, C.-C. Yang, F.-J. Lin
Journal of Food and Drug Analysis
Peroxisome proliferator-activated receptor α (PPARα) is a nuclear hormone receptor that transcriptionally regulates lipid metabolism and inflammation; therefore, PPARα agonists are promising agents to treat dyslipidemia and metabolic disorders. PPARα full agonists, such as fibrates, are effective anti-hypertriglyceride agents, but their use is limited by adverse side effects. Hence, the aim of this study was to identify small molecules that can activate PPARα while minimizing the adverse effects. Antrodia cinnamomea, a rare medical mushroom, has been used widely in Asian countries for the treatment of various diseases, including liver diseases. Antcin B, H and K (antcins) and ergostatrien-3β-ol (EK100) are …
