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Comparative Study On Amount Of Nutraceuticals In By-Products From Solvent And Cold Pressing Methods Of Rice Bran Oil Processing, D. Wongwaiwech, M. Weerawatanakorn, S. Tharatha, C.-T. Ho 2020 Taiwan Food and Drug Administration

Comparative Study On Amount Of Nutraceuticals In By-Products From Solvent And Cold Pressing Methods Of Rice Bran Oil Processing, D. Wongwaiwech, M. Weerawatanakorn, S. Tharatha, C.-T. Ho

Journal of Food and Drug Analysis

Rice bran oil (RBO) has become a popular oil globally. However, the RBO extraction process leaves various residue products, which contain bioactive substances of varying potency which could be significant sources of functional ingredients for both food production and pharmaceutical manufacture. The objective of our study was to compare the bioactive substances in various by-products derived from the two rice bran oil processing methods; solvent extraction and cold pressing. The residues from solvent extraction processing contained up to 97.37 mg/100 g of γ-aminobutyric acid in defatted rice bran, and the rice acid oil contained high levels of vitamin E (tocopherols, …


Raman Spectroscopy Coupled With Chemometric Methods For The Discrimination Of Foreign Fats And Oils In Cream And Yogurt, Karacaglar N.N. Yazgan, T. Bulat, I.H. Boyaci, A. Topcu 2020 Taiwan Food and Drug Administration

Raman Spectroscopy Coupled With Chemometric Methods For The Discrimination Of Foreign Fats And Oils In Cream And Yogurt, Karacaglar N.N. Yazgan, T. Bulat, I.H. Boyaci, A. Topcu

Journal of Food and Drug Analysis

The adulteration of milk fat in dairy products with cheaper non-milk based fats or oils is frequently encountered in the dairy industry. In this study, Raman spectroscopy with chemometric was used for the discrimination of foreign fats and oils in milk cream and yogurt. Firstly, binary mixtures of cream and oils (corn and sunflower oil), and vegetable fat blends which are potentially or currently used by the dairy industry were prepared. All fat or oil samples and their binary mixtures were examined by using Raman spectroscopy. Then, fat content of skim milk was adjusted to 3% (w/w) by the milk …


Prioritization Of Pesticides In Crops With A Semi-Quantitative Risk Ranking Method For Taiwan Postmarket Monitoring Program, W.-C. Chou, W.-R. Tsai, H.-H. Chang, S.-Y. Lu, K.-F. Lin, P. Lin 2020 Taiwan Food and Drug Administration

Prioritization Of Pesticides In Crops With A Semi-Quantitative Risk Ranking Method For Taiwan Postmarket Monitoring Program, W.-C. Chou, W.-R. Tsai, H.-H. Chang, S.-Y. Lu, K.-F. Lin, P. Lin

Journal of Food and Drug Analysis

A risk-based prioritization of chemical hazards in monitoring programs allows regulatory agencies to focus on the most potentially concerned items involving human health risk. In this study, a risk-based matrix, with a scoring method using multiple factors for severity and probability of exposure, was employed to identify the pesticides presented in crops that may pose the greatest risk to human health. Both the probability of exposure and the severity were assessed for 91 pesticides detected in a Taiwanese postmarketing monitoring program. Probability of exposure was evaluated based on the probability of consumption and evidence of pesticide residues in crops. Severity …


Effect Of Sinapic Acid On Aripiprazole Pharmacokinetics In Rats: Possible Food Drug Interaction, M. Raish, A. Ahmad, M.A. Ansari, K.M. Alkharfy, A. Ahad, A. Khan, F.I. Aljenobi, N. Ali, A.M. Al-Mohizea 2020 Taiwan Food and Drug Administration

Effect Of Sinapic Acid On Aripiprazole Pharmacokinetics In Rats: Possible Food Drug Interaction, M. Raish, A. Ahmad, M.A. Ansari, K.M. Alkharfy, A. Ahad, A. Khan, F.I. Aljenobi, N. Ali, A.M. Al-Mohizea

Journal of Food and Drug Analysis

Dietary supplements and foods can interact with various drugs, leading to possible clinical concerns. This study aimed to investigate the effect of orally administered sinapic acid (SA) on the pharmacokinetics of aripiprazole (APZ) in rats and its possible modulatory effects on hepatic cytochrome P450 (CYP3A2 and CYP2D6) expression in the liver tissues. Single dose and multiple dose parallel groups of wistar rats were categorized into six groups (n = 6 each) which abstained from food for 12 h prior to the experiment, while water was allowed ad libitum. The investigation was carried out for single dose: Group I was treated …


Catechin Rich Butanol Fraction Extracted From Acacia Catechu L. (A Thirst Quencher) Exhibits Immunostimulatory Potential, M.A. Sunil, V.S. Sunitha, A. Ashitha, S. Neethu, S.J. Midhun, E.K. Radhakrishnan, M. Jyothis 2020 Taiwan Food and Drug Administration

Catechin Rich Butanol Fraction Extracted From Acacia Catechu L. (A Thirst Quencher) Exhibits Immunostimulatory Potential, M.A. Sunil, V.S. Sunitha, A. Ashitha, S. Neethu, S.J. Midhun, E.K. Radhakrishnan, M. Jyothis

Journal of Food and Drug Analysis

Acacia catechu L., (Fabaceae) named as “catechu” is a plant, the decoction of heartwood of which is daily consumed as thirst quencher by a good percentage of the population in South India. The plant is mainly distributed in India and other Asian countries. It has been used in Indian traditional medicine for the treatment of asthma, bronchitis, colic, diarrhea, boils, skin afflictions, sores and stomatitis. The present investigation was aimed to study the immunomodulatory effects of different fractions of ethanol extract of A. catechu heartwood and HPLC analysis of the active fraction. Three fractions namely, butanol, chloroform and ethyl acetate …


Multiclass Analysis Of Antimicrobial Drugs In Shrimp Muscle By Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry, S. Susakate, S. Poapolathep, C. Chokejaroenrat, P. Tanhan, J. Hajslova, M. Giorgi, K. Saimek, Z. Zhang, A. Poapolathep 2020 Taiwan Food and Drug Administration

Multiclass Analysis Of Antimicrobial Drugs In Shrimp Muscle By Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry, S. Susakate, S. Poapolathep, C. Chokejaroenrat, P. Tanhan, J. Hajslova, M. Giorgi, K. Saimek, Z. Zhang, A. Poapolathep

Journal of Food and Drug Analysis

A reliable, selective and rapid multiclass method has been developed for the simultaneous determination of 55 antibacterial drug residues in shrimp muscle samples by ultra high performance liquid chromatography-tandem mass spectrometry. The investigated compounds comprise of eight different classes, namely fluoroquinolones, sulfonamides and synergistic agents, tetracyclines, macrolides, lincosamides, penicillins, nitroimidazole and amphenicols. A simple liquid extraction procedure was developed consisting of extraction with a mixture of acetonitrile and ethylenediaminetetraacetic acid (EDTA), followed by a defatting step with n-hexane. Chromatographic conditions were optimized, obtaining a running time 74.3% to 113.3%. For precision test, relative standard deviations (RSD, %) were lower than …


Rgo-Ns Sers-Based Coupled Chemometric Prediction Of Acetamiprid Residue In Green Tea, M.M. Hassan, Q. Chen, F.Y.H. Kutsanedzie, H. Li, M. Zareef, Y. Xu, M. Yang, A.A. Agyekum 2020 Taiwan Food and Drug Administration

Rgo-Ns Sers-Based Coupled Chemometric Prediction Of Acetamiprid Residue In Green Tea, M.M. Hassan, Q. Chen, F.Y.H. Kutsanedzie, H. Li, M. Zareef, Y. Xu, M. Yang, A.A. Agyekum

Journal of Food and Drug Analysis

Pesticide residue in food is of grave concern in recent years. In this paper, a rapid, sensitive, SERS (Surface-enhanced Raman scattering) active reduced-graphene-oxide-gold-nano-star (rGO-NS) nano-composite nanosensor was developed for the detection of acetamiprid (AC) residue in green tea. Different concentrations of AC combined with rGO-NS nano-composite electro-statically, yielded a strong SERS signal linearly with increasing concentration of AC ranging from 1.0 × 10−4 to 1.0 × 103 μg/mL indicating the potential of rGO-NS nano-composite to detect AC in green tea. Genetic algorithm-partial least squares regression (GA-PLS) algorithm was used to develop a quantitative model for AC residue prediction. The GA-PLS …


Development And Validation Of A New Analytical Hplc Method For Simultaneous Determination Of The Antidiabetic Drugs, Metformin And Gliclazide, A. Gedawy, H. Al-Salami, C.R. Dass 2020 Taiwan Food and Drug Administration

Development And Validation Of A New Analytical Hplc Method For Simultaneous Determination Of The Antidiabetic Drugs, Metformin And Gliclazide, A. Gedawy, H. Al-Salami, C.R. Dass

Journal of Food and Drug Analysis

An efficient and simple HPLC method has been developed and validated for the simultaneous determination of gliclazide and metformin hydrochloride in bulk and was applied on marketed metformin and gliclazide products. The mobile phase used for the chromatographic runs consisted of 20 mM ammonium formate buffer (pH 3.5) and acetonitrile (45:55, v/v) The separation was achieved on an Alltima CN (250 mm × 4.6 mm x5μ) column using isocratic mode. Drug peaks were well separated and were detected by a UV detector at 227 nm. The method was linear at the concentration range 1.25–150 μg/ml for gliclazide and 2.5–150 μg/ml …


A Rapid Lc-Ms/Ms Method For Simultaneous Determination Of Quetiapine And Duloxetine In Rat Plasma And Its Application To Pharmacokinetic Interaction Study, X. Chen, C. Liang, L. Cui, J. Le, Z. Qian, R. Zhang, Z. Hong, Y. Chai 2020 Taiwan Food and Drug Administration

A Rapid Lc-Ms/Ms Method For Simultaneous Determination Of Quetiapine And Duloxetine In Rat Plasma And Its Application To Pharmacokinetic Interaction Study, X. Chen, C. Liang, L. Cui, J. Le, Z. Qian, R. Zhang, Z. Hong, Y. Chai

Journal of Food and Drug Analysis

Combinations of new antidepressants like duloxetine and second-generation antipsychotics like quetiapine are used in clinical treatment of major depressive disorder, as well as in forensic toxicology scenarios. The drug–drug interaction (DDI) between quetiapine and duloxetine is worthy of attention to avoid unnecessary adverse effects. However, no pharmacokinetic DDI studies of quetiapine and duloxetine have been reported. In the present study, a rapid and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of quetiapine and duloxetine in rat plasma. A one-step protein precipitation with acetonitrile was applied for sample preparation. The analytes were eluted on an …


Abelmoschus Esculentus Subfractions Improved Nephropathy With Regulating Dipeptidyl Peptidase-4 And Type 1 Glucagon-Like Peptide Receptor In Type 2 Diabetic Rats, C.-H. Peng, H.-C. Lin, C.-L. Lin, C.-J. Wang, C.-N. Huang 2020 Taiwan Food and Drug Administration

Abelmoschus Esculentus Subfractions Improved Nephropathy With Regulating Dipeptidyl Peptidase-4 And Type 1 Glucagon-Like Peptide Receptor In Type 2 Diabetic Rats, C.-H. Peng, H.-C. Lin, C.-L. Lin, C.-J. Wang, C.-N. Huang

Journal of Food and Drug Analysis

Abelmoschus esculentus (AE) has been used in traditional medicine to ameliorate hyperglycemia, but its mucilage increased bioassay difficulties. We have obtained a series of AE subfractions. Among them F1 and F2 regulated dipeptidyl peptidase-4 (DPP-4) and type 1 glucagon-like peptide receptor (GLP-1R), the treatment targets for type 2 diabetes. F1, F2 and fraction residues (FR) showed advantage on different aspects, which attenuates insulin resistance and metabolic disorder in vivo, and prevents renal-tubular change in vitro. In the present study, using type 2 diabetes model induced by high fat diet (HFD) and streptozotocin (STZ), we aim to investigate whether AE prevent …


Encapsulation Of Babchi Essential Oil Into Microsponges: Physicochemical Properties, Cytotoxic Evaluation And Anti-Microbial Activity, G. Wadhwa, S. Kumar, V. Mittal, R. Rao 2020 Taiwan Food and Drug Administration

Encapsulation Of Babchi Essential Oil Into Microsponges: Physicochemical Properties, Cytotoxic Evaluation And Anti-Microbial Activity, G. Wadhwa, S. Kumar, V. Mittal, R. Rao

Journal of Food and Drug Analysis

Babchi essential oil (BEO) is a valuable essential oil reported to possess a variety of biological activities such as antitumor, anti inflammatory, immunomodulatory, antioxidant, antifungal and antibacterial properties. Due to its anti-microbial properties, this oil possesses an immense potential for the treatment of dermatological disorders. Further, it has minimal tendency to develop resistance, a common issue with most of the antibiotics. However, its highly viscous nature and poor stability in the presence of light, air and high temperature, limits its practical applications. To surmount these issues, this research aims to encapsulate BEO in ethyl cellulose (EC) microsponges for enhanced stability, …


Rubiscolin-6 Activates Opioid Receptors To Enhance Glucose Uptake In Skeletal Muscle, T.S. Kairupan, K.-C. Cheng, A. Asakawa, H. Amitani, T. Yagi, K. Ataka, N.T. Rokot, N.H. Kapantow, I. Kato, A. Inui 2020 Taiwan Food and Drug Administration

Rubiscolin-6 Activates Opioid Receptors To Enhance Glucose Uptake In Skeletal Muscle, T.S. Kairupan, K.-C. Cheng, A. Asakawa, H. Amitani, T. Yagi, K. Ataka, N.T. Rokot, N.H. Kapantow, I. Kato, A. Inui

Journal of Food and Drug Analysis

Rubiscolin-6 is an opioid peptide derived from plant ribulose bisphosphate carboxylase/oxygenase (Rubisco). It has been demonstrated that opioid receptors could control glucose homeostasis in skeletal muscle independent of insulin action. Therefore, Rubiscolin-6 may be involved in the control of glucose metabolism. In the present study, we investigated the effect of rubiscolin-6 on glucose uptake in skeletal muscle. Rubiscolin-6-induced glucose uptake was measured using the fluorescent indicator 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxyglucose (2-NBDG) in L6 and C2C12 cell lines. The protein expressions of glucose transporter 4 (GLUT4) and AMP-activated protein kinase (AMPK) in L6 cells were observed by Western blotting. The in vivo effects …


The Rapid And Sensitive Detection Of Edible Bird's Nest (Aerodramus Fuciphagus) In Processed Food By A Loop-Mediated Isothermal Amplification (Lamp) Assay, M.-S. Lee, J.-Y. Huang, Y.-Y. Lien, S.-C. Sheu 2020 Taiwan Food and Drug Administration

The Rapid And Sensitive Detection Of Edible Bird's Nest (Aerodramus Fuciphagus) In Processed Food By A Loop-Mediated Isothermal Amplification (Lamp) Assay, M.-S. Lee, J.-Y. Huang, Y.-Y. Lien, S.-C. Sheu

Journal of Food and Drug Analysis

Edible bird's nest (EBN) is a well-known and precious traditional Chinese herbal material (CHM). Because of this, preventing the adulteration of EBN efficiently and precisely is crucial to protect consumers’ interests and health. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for the detection of EBN using specifically designed LAMP primers. The results demonstrated that the identification of EBN by LAMP assay was specific and rapid (within 1 h). It had no cross-reaction with EBN adulterants, including white fungus, egg white and pig skin, in different ratios. The relative detection limit was 0.01% EBN in the adulterants. …


Ubiquitin-Protein Ligase E3a (Ube3a) As A New Biomarker Of Cardiac Hypertrophy In Cell Models, K.-C. Cheng, Y. Li, W.-T. Chang, Z.-C. Chen, J.-T. Cheng, C.-C. Tsai 2020 Taiwan Food and Drug Administration

Ubiquitin-Protein Ligase E3a (Ube3a) As A New Biomarker Of Cardiac Hypertrophy In Cell Models, K.-C. Cheng, Y. Li, W.-T. Chang, Z.-C. Chen, J.-T. Cheng, C.-C. Tsai

Journal of Food and Drug Analysis

Cardiac hypertrophy is widely diagnosed in clinical cardiac disorders. The pathophysiology of hypertrophy is complex and multifactorial, a series of molecular and cellular changes are participated, such as activation of different signaling pathways, a switch of fetal gene program in the myocardium, and apoptosis. Some biomarkers have been applied to assess cardiac hypertrophy including atrial natriuretic peptides (ANP), brain/B-type natriuretic peptides (BNP), and α- or β- Myosin Heavy Chain (MHC) in addition to others. Recently, ubiquitin-protein ligase E3A (UBE3A) has been observed to increase in cardiac hypertrophy. Therefore, UBE3A as a new biomarker seems valuable in the clinic. The cardiac …


Antidepressant And Anxiolytic Effects Of The Proprietary Chinese Medicine Shexiang Baoxin Pill In Mice With Chronic Unpredictable Mild Stress, X.-D. Zhou, D.-D. Shi, Z.-J. Zhang 2020 Taiwan Food and Drug Administration

Antidepressant And Anxiolytic Effects Of The Proprietary Chinese Medicine Shexiang Baoxin Pill In Mice With Chronic Unpredictable Mild Stress, X.-D. Zhou, D.-D. Shi, Z.-J. Zhang

Journal of Food and Drug Analysis

Depression and anxiety often co-occur with cardiac diseases. The Shexiang Baoxin pill (SBP) is a proprietary Chinese medicine initially used to treat cardiac conditions. This study explored whether SBP has antidepressant and anxiolytic effects in addition to hormonal and psychotropic mechanisms. Mice underwent 6 weeks of chronic unpredictable mild stress (CUMS) to induce depression- and anxiety-like behavior. During the 6-week experiment, mice received SBP at intragastric doses of 20.25 mg/kg or 40.5 mg/kg daily. Animals were then tested for depression in sucrose preference, forced-swimming, and tail suspension paradigms, and for anxiety in open field and elevated plus maze tests. Both …


Novel Technologies In Detection, Treatment And Prevention Of Substance Use Disorders, H. Moulahoum, F. Zihnioglu, S. Timur, H. Coskunol 2020 Taiwan Food and Drug Administration

Novel Technologies In Detection, Treatment And Prevention Of Substance Use Disorders, H. Moulahoum, F. Zihnioglu, S. Timur, H. Coskunol

Journal of Food and Drug Analysis

Substance use disorders are a widely recognized problem, which affects various levels of communities and influenced the world socioeconomically. Its source is deeply embedded in the global population. In order to fight against such an adversary, governments have spared no efforts in implementing substance abuse treatment centers and funding research to develop treatments and prevention procedures. In this review, we will discuss the use of immunological-based treatments and detection kit technologies. We will be detailing the steps followed to produce performant antibodies (antigens, carriers, and adjuvants) focusing on cocaine and methamphetamine as examples. Furthermore, part of this review is dedicated …


Determination Of Sulfamonomethoxine In Tilapia (Oreochromis Niloticus × Oreochromis Mossambicus) By Liquid Chromatography-Tandem Mass Spectrometry And Its Application Pharmacokinetics Study, T.-A. Kung, S.-H. Lee, W.-H. Wang 2020 Taiwan Food and Drug Administration

Determination Of Sulfamonomethoxine In Tilapia (Oreochromis Niloticus × Oreochromis Mossambicus) By Liquid Chromatography-Tandem Mass Spectrometry And Its Application Pharmacokinetics Study, T.-A. Kung, S.-H. Lee, W.-H. Wang

Journal of Food and Drug Analysis

A precise and reliable analytical method to measure trace levels of sulfamonomethoxine (SMM) and N4-acetyl metabolite in tilapia samples using liquid chromatography-tandem mass spectrometry was developed. Optimized chromatographic separation was performed on C18 reversed-phase columns using gradient elution with methanol and 5 mmol/L of an ammonium acetate aqueous solution (adjusted to pH 3.5 using formic acid). This study investigated the pharmacokinetic properties and tissue distribution of SMM and its major metabolite N4-acetyl sulfamonomethoxine (AC-SMM) in tilapia after a single dose of 100 mg kg−1 body weight of orally administered SMM. Blood and tissues were collected between 0.5 and 192 h …


Identification Of Baicalin From Bofutsushosan And Daisaikoto As A Potent Inducer Of Glucose Uptake And Modulator Of Insulin Signaling-Associated Pathways, Y.-T. Kuo, C.-C. Lin, H.-T. Kuo, J.-H. Hung, C.-H. Liu, A. Jassey, M.-H. Yen, S.-J. Wu, L.-T. Lin 2020 Taiwan Food and Drug Administration

Identification Of Baicalin From Bofutsushosan And Daisaikoto As A Potent Inducer Of Glucose Uptake And Modulator Of Insulin Signaling-Associated Pathways, Y.-T. Kuo, C.-C. Lin, H.-T. Kuo, J.-H. Hung, C.-H. Liu, A. Jassey, M.-H. Yen, S.-J. Wu, L.-T. Lin

Journal of Food and Drug Analysis

Type 2 diabetes mellitus (T2DM) is a chronic disease characterized by hyperglycemia that can lead to long-term complications including heart diseases, stroke, retinopathy, and renal failure. Treatment strategies include stimulating glucose uptake and controlling blood glucose level. Bofutsushosan (BOF) and Daisaikoto (DAI) are two herb-based kampo medicines that have been demonstrated to improve metabolism-associated disorders including obesity, hyperlipidemia, and nonalcoholic fatty liver. Given their bioactivities against metabolic syndromes, we explored in this study the effect of BOF and DAI extracts on glucose absorption and used them as source to identify phytochemical stimulator of glucose absorption. Glucose uptake and mechanistic studies …


Rapid Screening Of Sulfonamides In Dietary Supplements Based On Extracted Common Ion Chromatogram And Neutral Loss Scan By Lc-Q/Tof-Mass Spectrometry, N.-Y. Ki, J. Hur, B.H. Kim, K.H. Kim, B.J. Moon, H.B. Oh, J. Hong 2020 Taiwan Food and Drug Administration

Rapid Screening Of Sulfonamides In Dietary Supplements Based On Extracted Common Ion Chromatogram And Neutral Loss Scan By Lc-Q/Tof-Mass Spectrometry, N.-Y. Ki, J. Hur, B.H. Kim, K.H. Kim, B.J. Moon, H.B. Oh, J. Hong

Journal of Food and Drug Analysis

There is an increasing amount of dietary supplements that are adulterated with diuretics and anti-diabetic drugs; this has become a global problem due to the wide distribution of dietary supplements and the serious negative health effects of the adulterants. In this study, a rapid screening method was developed for detection and confirmation of 35 sulfonamides in supplements by ultra-high performance liquid chromatography quadrupole/time of flight mass spectrometry. For effective extraction of sulfonamides from dietary supplements, four extraction protocols including HLB and WAX solid-phase extraction, Quick Easy Cheap Effective Rugged and Safe method, and pH-controlled liquid–liquid extraction were evaluated, and pH-controlled …


In Vivo Pro-Angiogenic Effects Of Dracorhodin Perchlorate In Zebrafish Embryos: A Novel Bioactivity Evaluation Platform For Commercial Dragon Blood Samples, P. Krishnaraj, Y. Chang, T.-J. Ho, N.-C. Lu, M.-D. Lin, H.-P. Chen 2020 Taiwan Food and Drug Administration

In Vivo Pro-Angiogenic Effects Of Dracorhodin Perchlorate In Zebrafish Embryos: A Novel Bioactivity Evaluation Platform For Commercial Dragon Blood Samples, P. Krishnaraj, Y. Chang, T.-J. Ho, N.-C. Lu, M.-D. Lin, H.-P. Chen

Journal of Food and Drug Analysis

Dragon blood has been used in wound treatment for many years and can be obtained from several distinct plant species. Dracorhodin, the active substituent of dragon blood, is a characteristic compound of the palm tree, Daemonorops draco. At present, the only method to evaluate the quality of commercial dragon blood samples is a HPLC method which determines the amount of dracorhodin in a dragon blood sample. In this study, we used zebrafish embryos as a platform to demonstrate the in vivo pro-angiogenic activity of dracorhodin perchlorate, the chemically synthesized analog of dracorhodin. By using this platform, three different commercial dragon …


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