Food Science Commons^™

Systematic Verification Of Saliva-Based Biosignatures For Oral Cancer Via Targeted Metabolomics, Ya-Ju Hsieh, Yu Chang, Chi Yang, Nai Hsuan Teng, Jau-Song Yu, Kai-Ping Chang, Wen-Chen Chen, Lichieh Julie Chu, Yi-Ting Chen

Journal of Food and Drug Analysis

Oral squamous cell carcinoma (OSCC) remains a major global health challenge, and early detection is essential for improving patient outcomes. To identify reliable salivary biomarkers, we performed a targeted metabolomics study using ¹³C₂/¹²C₂-dansylation, integrating candidate metabolites from published OSCC metabolomics datasets and systematically verifying them through LC-MRM-MS. A panel of 26 metabolites was quantified in saliva samples from 299 subjects, including healthy controls (n = 98), oral potentially malignant disorders (OPMD I, n = 50; OPMD II, n = 53), and OSCC patients (n = 98). Among these, cadaverine, N-acetylcadaverine, choline, glycine, and …

Phthalate Exposure And Lipid Disruption In Taiwanese Children: Insights From Serum Lipidomic Approach, Tzu-Hsin Yen, Wei-Chen Tseng, Sheng-Han Lee, Zhi-Yi Du, Wan-Yu Lin, Mei-Huei Chen, Ching-Chun Lin, Ching-Chun Lin, Hui-Ju Wen, Hao-Jan Liang, Yue-Leon Guo, Pau-Chung Chen, Ching-Yu Lin

Journal of Food and Drug Analysis

Phthalates are ubiquitous environmental contaminants that raise particular concern for children, who generally experience higher exposure levels and are more susceptible to environmental pollutants. To understand potential molecular mechanisms, we conducted a cross-sectional study of 256 Taiwanese children aged 8–10 years from two population-based cohorts: the Taiwan Birth Panel Study and the Taiwan Early-Life Cohort. Serum phosphocholine-containing lipids were profiled by liquid chromatography coupled with triple quadrupole mass spectrometry and associated with urinary levels of 12 phthalate metabolites. Multiple linear regression models, adjusted for demographic and socioeconomic factors, revealed that mono-(2-ethyl-5-hydroxyhexyl) phthalate (MEHHP), an oxidative metabolite of di(2-ethylhexyl) phthalate (DEHP), …

Applications Of Nuclear Magnetic Resonance Spectroscopy In Pediatric Clinical Metabolomics: From Research To Future Perspectives, Chieh-Ni Kuo, Meng-Han Chiang, Hsien-Ju Lee, Yu-Ying Yu, Eric Yi-Liang Shen, Chih-Yung Chiu

Journal of Food and Drug Analysis

Metabolomics studies small-molecule metabolites to provide insights into health and disease, supporting early diagnosis and personalized medicine. Advances in mass spectrometry and nuclear magnetic resonance (NMR) have expanded its use in metabolic, cancer, and cardiovascular diseases. In pediatrics, high-resolution NMR metabolomics has been instrumental in identifying age-related metabolic changes during early childhood and their associations with growth, nutrition, and disease risk. However, a comprehensive review of its clinical applications and future potential remains limited. This review highlights how utilizing specific NMR pulse sequences, such as CPMG and NOESY, allows for precise and non-destructive metabolic profiling of diverse biofluids, supported by …

The Evolution Of Desi-Msi: A Comprehensive Look At Preprocessing And Quantification Strategies For Metabolomics, Cheng-Hung Yang, Hung-Yao Ho, Chen-Yu Chang, Mei-Ling Cheng

Journal of Food and Drug Analysis

Mass spectrometry imaging (MSI) has become an indispensable tool in metabolomics for visualizing the spatial distribution of biomolecules within tissues. Due to its direct, ambient analysis capability, desorption electrospray ionization (DESI-MSI) has evolved from a rapid screening tool into a precise and reliable quantitative platform. We delve into its technical principles and particularly review the criticality of sample preprocessing, highlighting the paradigm shift from a "no-preprocessing" approach to one of "optimized preprocessing." The paper details how factors like sample preservation, choice of embedding agents, washing, drying, and on-tissue chemical derivatization all impact data quality. We also discuss how the addition …

Advanced Technologies Of Single-Cell Metabolomics Unveiling Cellular Metabolic Heterogeneity For Biological And Biomedical Research, Shang-Che Kuo, Ting-Wei Tsao, I-Fan Wang, Ying-Lan Chen

Journal of Food and Drug Analysis

Metabolomics provides direct insights into cellular physiology, yet it faces greater analytical challenges compared to genomics and transcriptomics due to the chemical diversity, instability, and environmental sensitivity of metabolites. Conventional bulk metabolomics averages signals across cell populations, thereby masking cellular heterogeneity critical for understanding disease mechanisms. Recent breakthroughs in single-cell metabolomics (SCM), driven by advances in mass spectrometry, microfluidics, isotope tracing, and spatial omics, have enabled the detection of metabolic diversity at unprecedented resolution. SCM has uncovered cell-type-specific biomarkers, revealed metabolic reprogramming in cancer and immunity and revealed disease progression. These studies highlight SCM’s transformative potential in biomarker discovery, clinical …

Metabolomics Of Per- And Polyfluoroalkyl Substances (Pfas) Exposure: Advances In Lc-Ms Technology And Applications, Yu-Meng Wang, Pei-Chen Lin, Yu-Da Zheng, Pin-Shiuan Wu, Kuo-Tai Hua, Sung-Fang Chen

Journal of Food and Drug Analysis

Per- and polyfluoroalkyl substances (PFAS) are a class of synthetic chemicals known for their high stability and bioaccumulation potential, leading to their widespread presence in the environment and human tissues. Exposure to PFAS has been associated with significant health concerns, including neurotoxicity, hepatotoxicity, and metabolic disturbances. Liquid chromatography–mass spectrometry (LC-MS) based metabolomics has emerged as a powerful and central tool for PFAS research, enabling the comprehensive analysis of metabolic changes induced by exposure. This review provides a critical summary of the applications of LC-MS metabolomics in both clinical studies and various experimental models, including in vivo models (mice) and in …

Recent Advances In Sustainable Production Of Enriched Chicken Eggs: A Mini Review, Karim El-Sabrout, Ayman Abd El-Aziz

Jurnal Ilmu Ternak Universitas Padjadjaran

Enriched or “designer” chicken eggs have emerged as an important category of functional foods aimed at improving human nutrition through dietary manipulation of laying hens. This mini-review summarizes recent advances in egg enrichment strategies, focusing on omega-3 fatty acids, vitamins, minerals, and antioxidant compounds. Omega-3 enrichment remains the most widely adopted approach, achieved through supplementation with flaxseed, fish oil, or microalgae, resulting in significant increases in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in egg yolk. Multi-nutrient enrichment, including selenium, vitamin E, and carotenoids, has also gained attention due to its potential in enhancing antioxidant capacity and health of hens …

Effect Of Dietary Onion Peel Powder (Allium Cepa L.) As A Feed Additive On Production Performance In Broiler, Della Ariesba Putri Dulia, Edhy Sudjarwo, Osfar Sjofjan

Jurnal Ilmu Ternak Universitas Padjadjaran

This study aims to evaluate the use of onion peel powder (Allium cepa L) as a feed additive on feed intake, weight gain, Feed Conversion Ratio (FCR), Production Index, and Income Over Feed Cost (IOFC) in broilers. A total of 144 Lohmann strain broiler (unsexed) were treated from 1 to 35 days of age. There were 6 treatments with 4 replicates, consisting of 24 experimental cages, each filled 6 broilers. The experimental design used completely randomized design (CRD). The treatment groups were as follows: basal feed without antibiotics (P0-), basal feed + 0.1% antibiotics (zinc bacitracin) (P0+), basal feed + …

Effect Of Gowok (Syzygium Polycephalum) Fortification On The Physical Properties And Color Of Goat Milk Yogurt, Siti Rahmawati Zulaikhah, Triana Setyawardani, Juni Sumarmono

Jurnal Ilmu Ternak Universitas Padjadjaran

This study aimed to determine the effect of adding parts of gowok fruit (Syzygium polycephalum) on the physical properties of goat’s milk yogurt. A completely randomized design was applied with treatments consisting of fruit parts (rind, flesh of fruit, and whole seedless fruit) and addition levels (1, 2, and 3%) with three replications. Data were analyzed using analysis of variance followed by Duncan’s multiple range test (DMRT). The results showed that gowok addition had a significant effect (P<0.05) on viscosity, syneresis, and color parameters, but no significant effect (P>0.05) on firmness and work of penetration. Yogurt viscosity ranged from 349.33 to 825 mPa·s and decreased with increasing gowok levels, while …

Physicochemical, Emulsion Stability, And Microbiological Changes Of Light Mayonnaise Containing Soy Protein During Storage, Rifka Anindita Putri, Herly Evanuarini, Premy Puspitawati Rahayu, Khothibul Umam Al Awwaly, Veronica Margareta Ani Nugiartiningsih

Jurnal Ilmu Ternak Universitas Padjadjaran

Light mayonnaise is a low-fat emulsion product that is susceptible to changes in viscosity, emulsion instability, and chemical quality degradation during storage. The addition of functional additives such as soy protein is commonly applied to support product quality. This study aimed to evaluate the physicochemical properties, emulsion stability, and microbiological quality of light mayonnaise containing 1.5% soy protein during storage for 0, 5, 10, and 15 days. The parameters analyzed included viscosity, pH value, emulsion stability, peroxide value, emulsion droplets, and total plate count (TPC). The study was conducted using four storage treatments with five replicates, applying a completely randomized …

Physicochemical, Organoleptic, And Antioxidant Functional Characteristics Of Dangke Whey Protein Ice Cream Fortified With Butterfly Pea Flower Extract, Muh. Achyar Ardat, Raden Hilman Wirayudha, Ekajayanti Kining, Zakiah Wulandari, Irma Isnafia Arief

Jurnal Ilmu Ternak Universitas Padjadjaran

This study aims to develop a functional ice cream based on dangke whey pro‐ tein with the addition of butterfly pea flower extract. Four formulations were pre‐ pared by varying milk and whey proportions: P1 (100% milk), P2 (60% milk + 40% whey), P3 (40% milk + 60% whey), and P4 (100% whey), with 5% butterfly pea ex‐ tract added to each treatment. The results showed that higher whey substitution decreased pH and overrun values but prolonged the melting resistance of ice cream. Moisture content increased with whey proportion, whereas ash content remained relatively constant across treatments. Protein and fat …

Valorization Of Rice Bran For Functional Film Fabrication Using Cold Plasma For Food Packaging And Biomedical Applications, Saydul Md Safwa

Graduate Theses and Dissertations

Abstract Over the past few years, conventional plastic packaging materials have become increasingly concerned with their environmental impact. Therefore, the demand for bioplastics has been increasing significantly over the years. Rice milling, a process that modifies paddy rice into edible rice, generates by-products such as rice bran. Rice bran has cellulose, hemicellulose, lignin, and protein, which makes it a potential material for bioplastic fabrication. However, like most biodegradable films, rice bran films fall short when it comes to practical packaging applications. They often lack the strength, flexibility, moisture resistance, and barrier performance needed to compete with synthetic plastics. Most importantly, …

Effects Of Soil Contamination On The Efficacy Of Antimicrobial Washes For Contaminated In-Shell Pecans, Erin Ramsay

Graduate Theses and Dissertations

A study was done to evaluate the survival of Shiga toxin-producing Escherichia coli (STEC) on directly and soil-inoculated in-shell pecans over a 28-day period, determine STEC reductions after treatment with individual and sequential antimicrobial washes on direct and soil-inoculated in-shell pecans, and determine the cross-contamination potential of the spent pecan washes after a treatment. Pecans were directly and soil-inoculated with an STEC cocktail (O157:H7, O157:NM, O121, and O26). Direct inoculation was achieved by spraying the STEC cocktail on the pecans and homogenizing by hand for 2 min. For the soil-inoculation method, autoclaved pecan tree orchard soil was sprayed with the …

Evaluating Variety- And Market-Class-Specific Effects Of Dietary Pulses On Human Health, Jiayu Tang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

While pulses are globally recognized for their high levels of dietary fibers, protein, and polyphenols, research often focuses on characterizing the physiological effects of a single variety. This approach overlooks the substantial genetic and compositional diversity that exists across pulse varieties and individual market classes, which can differ extensively in nutrient content and structure. These compositional differences have the potential to produce distinct physiological and microbial responses in humans, yet variety- and market-class-specific effects remain poorly characterized.

This thesis uses two complementary research methodologies to investigate the health-relevant effects of different pulse varieties and market classes, providing foundational knowledge to …

Evaluating Relationships Between Immunometabolism And Excess Adiposity Using Anthropometric And Body Composition Measurements, Corina Grimaldo

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Metabolic dysfunction is a key contributor to the development of chronic disease and is associated with excessive adiposity. Though body mass index (BMI) is a common screening tool used to identify possible metabolic dysfunction, it does not consider fat distribution. Other tools are needed to assess body composition and determine fat distribution. In recent studies, it has been suggested that alterations in metabolic processes may occur prior to a clinical diagnosis, and the combination of body composition and blood-based markers can help identify those early changes. The overall objective of this study is to identify low-cost indicators of metabolic dysregulation …

Evaluating The Impact Of Region, Vintage, And Carbon Dioxide-Based Winemaking Techniques On Wine Quality Of Mediterranean Varietals, Coleman R. Imrisek

Master's Theses

Changing Vitis vinifera ripening patterns as a result of increasing climate variability has provided challenges against the production of wines with a balanced sensory profile, with adequate acidity, color, aroma, and texture. This thesis aimed to evaluate the ripening patterns and wine profiles of potentially climate-adaptive Mediterranean varietals in two different California Central Coast climates. Additionally, different carbon-dioxide based winemaking techniques were also assessed. Viognier and Grenache Noir grapes were sourced from the warmer, diurnal Paso Robles AVA and the cooler, marine Santa Maria AVA during the 2024 and 2025 growing seasons, with Syrah and Grenache Blanc grapes being only …

Evaluation Of Loading, Release, And Bioavailability Of Resveratrol Encapsulated In Porous Starch, Yu-Yun Wang

Graduate Theses and Dissertations

Resveratrol (Res) is a polyphenolic compound found in grapes, red wine, and berries, with health benefits such as antioxidant, anti-inflammatory, and cardioprotective properties. The poor stability and bioaccessibility of Res after consumption by humans require the development of delivery systems. Among many delivery systems, porous starch (PS) is a safe, cost-effective, and high in adsorption capacity encapsulation material for drugs and bioactive compounds sensitive to environmental factors like light and heat. Porous rice, corn, and potato starch have different granule sizes, pore volumes, and surface areas. However, the impact of these encapsulation capabilities remains unclear among the three porous starches. …

Soilless Substrates As A Critical Control Point For Mitigating Food Safety Risks Within Hydroponic Leaf Lettuce Production, Eric Nuertey Tetteh

Graduate Theses and Dissertations

The effect of hydroponic soilless substrates and microbial biocontrol agents on the persistence of foodborne pathogens in indoor lettuce production is variable depending on substrate type, pathogen species, and biocontrol formulation. This study addressed key gaps by first characterizing the ability of different substrates to support Salmonella and Listeria monocytogenes survival and then evaluating the ability of pine biochar and Bacillus subtilis to suppress pathogen persistence in selected substrates under controlled conditions. In the first experiment, the persistence of Salmonella and L. monocytogenes was evaluated in four soilless substrates: rockwool (RW), low density foam (LDF), peat moss (PM) and coco …

Valorisation Potential Of Microalgae As A Sustainable Source Of Lipids And Health Promoting Compounds, Bozena Mccarthy

Theses

The transition toward a circular bioeconomy requires sustainable strategies for managing and valorising food industry effluents while reducing reliance on environmentally damaging raw materials such as palm oil, which is associated with deforestation, biodiversity loss and greenhouse gas (GHGs) emissions. Microalgae represent a promising platform for converting nutrient rich industrial by-products into biomass and high-value compounds for food, nutraceutical and bio-based applications. Dairy processing generates large quantities of whey, creating environmental challenges due to its high organic load. Improved whey treatment and valorisation strategies are therefore essential for enhancing sustainability in food production systems. In this study, the biomass productivity …

Multiplex Pcr Assay To Distinguish Porcine And Non-Porcine Meats In Processed Foods Using Specific Primers From Nadh Dehydrogenase And Atp Synthase Gene, Kristianto Nugroho, Amalia Prihaningsih, Rerenstradika Tizar Terryana, Slamet Diah Volkandari, Mastur Mastur, Puji Lestari

Makara Journal of Science

Food safety issues, such as food adulteration between halal and non-halal meats, are among the most serious problems in Muslim countries like Indonesia. Food adulteration produces a mixture of products that cannot be detected visually by customers, and it could lead to several social and health problems. The availability of a detection method that can disti-nguish between porcine and non-porcine meats is very important, particularly one that can be utilized in small-scale labo-ratories. The aim of this study was to design and optimize species-specific primers that could distinguish between porcine and non-porcine meats in processed foods by utilizing NADH Dehydrogenase …