Food Science Commons^™

Lmpact Of Salmonella Enterica Effector Proteins On Plant Physiology And Immunity, Sara Qader

Honors Theses

Salmonella enterica serovar Typhimurium are primarily recognized as pathogens associated with foodborne illnesses in humans. Salmonella also can colonize plant tissue effectively. The pathogen relies on type III secretion systems (T3SS) to infect mammals and plants successfully and counteract the immune response. Understanding the molecular mechanisms underlying Salmonella’s interactions with plants is essential for developing effective strategies to reduce its impact on human health and agriculture. Effector proteins, known for their role in modulating host-pathogen interactions, have been implicated in the virulence and pathogenicity of Salmonella. However, their specific functions and contributions to Salmonella’s interactions with plants remain largely unknown. …

The Effect Of Activated Alkaline Earth Bentonite On The Quality Of Vegetable Oils, Nodirbek I. Boyjanov, Islombek I. Boyjanov, Аslbek B. Yulchiev, Kamar P. Serkayev

CHEMISTRY AND CHEMICAL ENGINEERING

The purpose of the research is to study the adsorption properties and mineralogical structure of alkaline earth bentonites from the Navbakhor deposit, as well as to develop technological regimes for their acid activation for use as bleaching clay in the purification of vegetable oils. The authors, based on the structural structure of the studied bentonites, achieved an increase in adsorption capacity from 35 cm³/g to 100 cm³/g, by treating with hydrochloric and sulfuric acids with a concentration of 15-20% and in a bentonite:acid ratio of 1:2.5 at a temperature below 100°С. Nitrogen adsorption isotherms of activated …

Influence Of Lipolytic Enzymes On The Fractional Composition Of Wheat Alcohol, Bobir S. Kayumov, Saida Sh. Khakimova, Khasan T. Khasanov

CHEMISTRY AND CHEMICAL ENGINEERING

The purpose of this work is to study the influence of lipolytic enzymes on the fermentation activity and fractional composition of alcohol by the yeast Saccharamyces cerevisea during alcoholic fermentation. Lipolytic enzymes, intensifying the fermentation process and increasing the yield of the target product, have a positive effect on the physiological activity of yeast cells. Hydrolysis of carboxylic acid esters also occurs due to the hydrolase activity of lipases. In the presence of exogenous lipases, hydrolysis of lipids occurs due to the hydrolase activity of lipases and the fractional composition of alcohol changes. The content of esters is reduced by …

Selection Of Optimal Conditions For The Drying Process Of An Aqueous Extract From The Aerial Part Of Scutellaria Adenostegia, Shohida A. Ergasheva, Muniraxon A. Mamatxanova, Temurbek A. Khajibaev, Ravshanjon M. Khalilov, Akhmatkhon U. Mamatkhanov

CHEMISTRY AND CHEMICAL ENGINEERING

In order to establish optimal drying modes for an aqueous extract from the aboveground part of Scutellaria adenostegia, the following parameters were studied: solution concentration; solution feed rate; temperature of the drying agent at the inlet and outlet; pressure when the solution is fed into the drying chamber. It was found that in order to obtain a dry extract from the aboveground part of Scutellaria adenostegia, antihypoxic action, an aqueous extract obtained with water at a temperature of 60 ° C must be concentrated to a content of 15% of dry substances and dried in a spray dryer of the …

Effects Of Meat Attachment And Social Normative Messaging On Consumer Acceptance Of A Blended Plant-Forward Sausage Patty, Benjamin Garza

Theses & Dissertations

OBJECTIVE, The consumption of red and processed meat in the U.S. diet is rooted in psychological and social determinants. While meat alternatives (e.g., Beyond Beef) have recently entered the food and beverage market, they are not well received by consumers. The purpose of this study was to examine the effect of meat attachment and social normative messages on consumer acceptance of a blended, plant-forward meat product. METHODS. Seventy-one university students (Age: M = 22.14; Gender: Female = 52) between the ages of 18-30 years and without food allergies were recruited. This study utilized a 2 (between-subjects; social normative messages) x …

Mathematical Evaluation Of Population Changes Of Lactobacillus Acidophilus And Bifidobacterium Animalis Ssp. Lactis As Free And Encapsulated Cells In Butter, Rakesh Kaushik, Kritika Gaba, Sanjeev Anand, Gemechis Djira

Dairy Science and Food Science Faculty Publications: 2022 to Present

Growing butter markets, domestically and globally, provide opportunities for value-added variants of butter. Adding probiotics to butter could boosts its bioactivity; however, maintaining probiotic viability during storage is a major challenge. Mathematical analysis of probiotic population changes could help improve our understanding of how probiotics interact with butter and storage conditions. Two strains of probiotics in a 1:1 ratio as free cells or Whey Protein Hydrolysate–Maltodextrin (WPH-MD)-encapsulated cells, Lactobacillus acidophilus ATCC 4356 (LA5) and Bifidobacterium animalis ssp. lactis ATCC 27536 (BB12), were separately mixed into butter at 1% levels. Using analysis of covariance, a mathematical evaluation for probiotic population changes …

Magnetic Three-Dimensional Graphene: A Superior Absorbent For Selective And Sensitive Determination Of Nitrite In Water Samples By Ion-Pair Based-Surfactant-Assisted Solid-Phase Extraction Combined With Spectrophotometry, Mina Vasheghani Farahani, Sajad Karami, Hassan Sereshti, Shokouh Mahpishanian, Somayeh Koupaei Malek, Shahabaldin Rezania

Nutrition, Dietetics, and Food Science Student Research

A straightforward, fast and efficient analytical method was developed which utilizes a magnetic composite called three-dimensional graphene (3D-G@Fe₃O₄) as an adsorbent to recover nitrite ions (NO₂^–) from environmental water samples. The investigation into the synthesized adsorbent contained an examination of its morphology, chemical composition, structural attributes, and magnetic properties. This comprehensive analysis was conducted using various instrumental techniques, including Fourier transform-infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), Raman spectroscopy, X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), Barrett-Joyner-Halenda (BJH), and vibrating sample magnetometry (VSM). The adsorbent surface was activated by adding cetyltrimethylammonium bromide (CTAB) to the …

Design Of Small-Scale Milk Processing Facility, Luke Bond, Robert Stenzel, Ry Steffen, John Van Nieuwenhuyse, Rossana Villa-Rojas, Terry Howell Jr., Tami M. Brown-Brandl, Forrest Kievit

Department of Agricultural and Biological Systems Engineering: Masters Project Reports

This project was intended to investigate the feasibility of a proposed modular dairy processing facility collocated with a small dairy on the University of Nebraska–Lincoln’s Innovation Campus. Priority was placed upon cost savings for equipment to be used in processing. Process flow diagrams, floor plans, production schedules, risk assessments, and plans for wastewater treatment were all developed to address the needs of the plant should it be built. A Monte Carlo simulation was conducted to evaluate the timeline for breaking even financially, resulting in the potential for a payback period of 4.5 years. The 4.5-year payback period was based on …

Moisture Sorption And Quality Characteristics Of Instant Rice, Anna Mays, Griffiths Atungulu

Food Science Undergraduate Honors Theses

Market demand for instant rice--a processed form of rice that is cooked and dehydrated before it is sold--has risen tremendously and become a significant component of the rice industry. Compared to freshly cooked rice, the quality of instant rice (in terms of texture, color, aroma, etc.) is much lower. There exists little information regarding how instant rice’s storage conditions affect its quality. Such information may be elucidated by studying sorption isotherms which describe the storage temperature, relative humidity, and instant rice moisture relationships.

The purpose of this study was to generate moisture sorption isotherms of instant rice for temperatures and …

Assessing Health Implications Of Monarch Butterfly Populations And Behaviors, Kira Goldman, Kelsey E. Fisher Dr.

Honors Scholar Theses

Pollinator populations have seen massive declines over the past decade due to anthropogenic activity and climate change posing serious threats to the health of humans and ecosystems (Ulyshen & Horn, 2023). As agents responsible for the production of about 35% of micronutrient rich food crops necessary for adequate nutrition to lead healthful lives and prevent nutrition associated diseases, pollinator decline is a public health concern (Aizen et al., 2009). Monarch butterflies (Danaus plexippus) serve as bio indicators for the health of pollinator populations because of their analogous habitat and food resources shared with many other pollinator species, including bees and …

Impact Of Water Combined Wet Ball Milling Extraction And Functional Evaluation Of Dietary Fiber From Papaya (Carica Papaya L), Guihun Jiang, Kashif Ameer, Karna Ramachandraiah, Xiaoyu Feng

School of Medicine Faculty Publications

This study evaluates the structural, physicochemical, functional and rheological properties of papaya dietary fibers (DFs) extracted by alkaline, water and combination of water/wet ball milling. The particle size of DF subjected to water/wet ball milling (WB-DF) was considerably reduced compared to DF extracted by water (W-DF) or alkaline (AL-DF) methods. WB-DF in comparison AL-DF increased the water holding capacity (WHC) by 4.1 folds, oil holding capacity (OHC) by 1.7 folds and water swelling capacity (WSC) by 2.6 folds. WB-DF also improved the cholesterol adsorption capacity (CAC), glucose adsorption (GAC), nitrite-ion adsorption capacity (NIAC) and antioxidant activity. The FT-IR spectra displayed …

Exopolysaccharides Of Lactic Acid Bacteria Isolated From Labneh: Characterization, Bioactivities, And Effects On Gut Microbiome And Fermented Milk Rheology, Mohammed Tarique

Dissertations

Exopolysaccharides (EPS), produced by Lactic Acid Bacteria (LAB), are carbohydrate polymers with unique physicochemical properties and various biological activities widely used in food and pharmaceutical industries. This dissertation is aimed to (1) screen 42 new isolates from traditional yogurt-like products (Labneh) for their probiotic nature and identify the LAB strains using 16S rDNA sequencing; (2) optimize the conditions like pH, temperature, time, and quantity of sucrose for the growth of EPS produced by four selected LAB isolates; (3) purify and characterize EPS for the contents and investigate the bioactivities of the partially purified EPS; (4) study the effects of the …

Bootstrapping For Estimating The Conservative Kill Ratio Of The Surrogate To The Pathogen For Use In Thermal Process Validation At The Industrial Scale, Arshpreet Kaur Khattra, Surabhi Wason, Kevin Thompson, Andy Mauromostakos, Jeyamkondan Subbiah, Jennifer C. Acuff

Food Science Faculty Publications and Presentations

A surrogate is commonly used for process validations. The industry often uses the target log cycle reduction for the test (LCR_Test) microorganism (surrogate) to be equal to the desired log cycle reduction for the target (LCR_Target) microorganism (pathogen). When the surrogate is too conservative with far greater resistance than the pathogen, the food may be overprocessed with quality and cost consequences. In aseptic processing, the Institute for Thermal Processing Specialists recommends using relative resistance (D_Target)/(D_Test) to calculate LCR_Test (product of LCR_Target and relative resistance). This method uses the mean values …

Exploring The Potential Of Electromagnetic-Driven Energy Technologies In Preventing Microbial And Aflatoxin Contamination Of Grains, Abass A. Oduola

Graduate Theses and Dissertations

Electromagnetic-driven energy such as infrared (IR) technology holds great potential in deactivating Aspergillus flavus spores and prevents aflatoxin B1 (AFB1) contamination. Aflatoxins, highly toxic secondary metabolites primarily produced by Aspergillus species within the Flavi section (A. flavus, A. parasiticus, and A. nomius), pose substantial health risks to both humans and animals due to their significant toxicity and carcinogenic properties. Detecting and quantifying the presence and proliferation of these harmful fungi on food materials can be achieved by measuring the concentration of ergosterol. Ergosterol is a sterol compound produced in the cell membranes of fungi during their growth, providing …

Design And Application Of Smart Systems To Address Analytical Problems, Lucas B. Ayres

All Dissertations

This dissertation is a multidisciplinary effort that integrates low-cost analytical instrumentation, redox chemistry, and artificial intelligence to overcome existing limitations in the fields of wearable sensing technology, Deep Eutectic Solvents (DES), and antioxidant chemistry. The overall goal behind each implemented strategy is to enhance the accuracy, efficiency, and accessibility of analytical processes and technologies. A general overview of the thesis, along with the research outcomes is included in Chapter One. The theoretical framework of this dissertation is presented in Chapter Two. Chapter Three describes the development of a wearable platform (sensor and instrumentation) to rapidly detect (~20 minutes) S. aureus …

Assessing Impact Of Food Structure On Oral Tribology And In-Vitro Digestion Of Dairy Products, Lamis Ali

All Graduate Theses and Dissertations, Fall 2023 to Present

In this research, we focused on understanding the critical elements impacting consumer experience and the nutritional value of dairy products, specifically their texture, mouthfeel, and protein breakdown in the gastrointestinal tract. Our study aimed to accomplish two main goals. First, we performed analysis of the tribological attributes of various commercially dairy products. The second objective was to investigate the process of disintegration and protein release in selected dairy products.

The study included an assessment of eight dairy products of varied consistencies: solid like cheddar, cheese curd, and parmesan; semi-solid such as cottage cheese, ricotta cheese, and yogurt; and liquid represented …

Determination Of Factors Influencing Microbial Food Safety Risks Of Additive Manufacturing And 3d Printing Of Food, Allyson Nichole Hamilton

Graduate Theses and Dissertations

The burgeoning field of 3D food printing introduces opportunities for customizing the physicochemical properties of food, offering tailored solutions in color, shape, and texture. Despite its potential, the implications of this technology on food safety, particularly the efficacy of manufacturer-recommended cleaning protocols for stainless steel food ink capsules, the transferability of pathogens from a contaminated food ink capsule into the food ink based on macronutrient composition of the food, and the impact of storage conditions on pathogen viability, have not been thoroughly investigated. This dissertation provides a comprehensive analysis of these aspects, focusing on the control of foodborne pathogens and …

Physico-Chemical, Organoleptic, And Antioxidant Properties Of Black Soybean Flour (Glycine Soja (L.) Merrit) Flakes With The Addition Of Bangle (Zingiber Purpureum Roxb.), Ika Dyah Kumalasari, Nurkhasanah Nurkhasanah, Sepitriani Sepitriani

Pharmaceutical Sciences and Research

Flakes are products that are generally made from cereals. In this study, we are interested to use black soybean (Glycine soja (L) Merrit) and bangle (Zingiber purpureum Roxb.) as flakes ingredients. Black soybeans have high antioxidant components, while bangle contains high antioxidant and fiber components, which enables it to capture free radicals. This study aimed to find the most favorable formula with good physical, chemical, and organoleptic characteristics, which also has the highest antioxidant activity. We used the Completely Randomized Design (CRD) method and the variation of formulation used was the ratio of black soybean flour to bangle …

Assessing Sustainability Within The Brewing Industry: A Literature Review And Benchmarking Analysis., Elijah Thomas Miller

Electronic Theses and Dissertations

The goals of this study were twofold: to further examine sustainability within the brewing industry through a literature analysis across four academic journals, and a benchmarking analysis of a Louisville, Kentucky brewery’s resource consumption and emissions production. Publications related to the brewing industry were examined for the presence of sustainability keywords: “efficiency, sustainability, carbon footprint, climate change and greenhouse gas”. Benchmarking examined the local brewery’s primary production facility to determine their performance compared to other surveyed breweries pursuing sustainability across the country. Literature examination found noticeable discrepancies between the appearance of sustainability topics within article abstracts and their discussion within …

Use Of A Novel Combination Of Multiplex Pcr And Dna Barcoding In Assessing Authenticity Of Ginseng Products, Diane Kim

Food Science (MS) Theses

Ginseng (Panax sp.) is a medicinal plant root used for its purported health benefits, primarily in East Asian countries. The COVID-19 pandemic led to a significant increase in sales of dietary supplements, including ginseng supplements, to purportedly support immune health and provide other health benefits. However, this heightened demand has subsequently increased the risk of adulteration in these dietary supplements. The objective of this study was to determine the efficacy of a novel combination of DNA barcoding and multiplex PCR to identify species in ginseng supplements. A total of 50 commercial ginseng supplements containing either Panax ginseng, Panax quinquefolius, or …