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Establishment Of A Hplc Method For Simultaneous Determination Of 11 Phenolic Acids In Germinated Black Wheat, Qian LI, Xiaodong LI, Ziyi YANG, Yueyao ZHANG, Rui LIU, Zhanglong YU, Yuanlin SUN 2025 Life Sciences Department , Yuncheng University , Yuncheng 044099 , China;College of Food Science and Engineering , Shanxi Agricultural University , Taigu 030801 , China;Shanxi Technology Innovation Center of High Value-Added Echelon Utilization of Premium Agro-Products , Yuncheng 044099 , China

Establishment Of A Hplc Method For Simultaneous Determination Of 11 Phenolic Acids In Germinated Black Wheat, Qian Li, Xiaodong Li, Ziyi Yang, Yueyao Zhang, Rui Liu, Zhanglong Yu, Yuanlin Sun

Journal of Food Science and Biotechnology

[Objective ] In order to study the effects of germination on the mass fractions of phenolic acids in black wheat,a method was established for simultaneous determination of 11 phenolic acids (gallic acid,protocatechuic acid,4-hydroxybenzoic acid,chlorogenic acid,vanillic acid,caffeic acid,syringic acid,p-coumaric acid,ferulic acid,sinapic acid,and salicylic acid ) in germinated black wheat based on high performance liquid chromatography (HPLC ).[Method ] The mobile phase composed of methanol (containing acetic acid at a volume fraction of 1%) and water (containing acetic acid at a volume fraction of 1%) was used for gradient elution at a flow rate of 1 mL/min,a detection wavelength of 280 nm,and …


Dual-Nozzle 3d Printing And Drying Characteristics Of A Hawthorn Snack Based On Dual-Material Combination Gel, Aimiao WANG, Bo WANG, Yuchuan WANG, Jiguang LIU 2025 School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China

Dual-Nozzle 3d Printing And Drying Characteristics Of A Hawthorn Snack Based On Dual-Material Combination Gel, Aimiao Wang, Bo Wang, Yuchuan Wang, Jiguang Liu

Journal of Food Science and Biotechnology

[Objective ] This study aims at solving the problems such as long drying time,single product shape,and poor 3D printing capacity (easy blockage and weak bracing ability ) in conventional processing of hawthorn snack products.[Method ] The low-sugar hawthorn jam and high-sugar banana jam were used as gel components.The 3D printing was carried out with the optimization of dual-material combination gel formula and the optimization of dual-nozzle 3D printing process.Furthermore,the drying characteristics of 3D printing were analyzed and compared with those of conventional dried hawthorn products.[Result ] The combination of hawthorn jam with the sugar mass fraction of 26% and banana …


Respiratory Characteristics Of Late-Maturing Sweet Cherry Cultivars And Their Influence By Temperature And Packaging Methods, Huanhuan ZHI, Yu DONG 2025 Academy of Agriculture and Forestry Sciences , Qinghai University , Xining 810016 , China;Laboratory for Research and Utilization of Qinghai-Tibet Plateau Germplasm Resources , Xining 810016 , China;Key Laboratory of Qinghai-Tibetan Plateau Biotechnology (Qinghai University ), Ministry of Education , Xining 810016 , China;Key Laboratory of Agricultural Integrated Pest Management , Xining 810016 , China

Respiratory Characteristics Of Late-Maturing Sweet Cherry Cultivars And Their Influence By Temperature And Packaging Methods, Huanhuan Zhi, Yu Dong

Journal of Food Science and Biotechnology

[Objective ] This study aims to develop suitable packaging strategies for sweet cherry varieties with different respiratory characteristics.[Method ] The sweet cherry cultivars ‘Lapins’ and ‘Regina’ were put in closed systems with different O 2 volume fractions at 0,5,and 10 ℃.The changes in O 2 consumption rate,CO 2 consumption rate,and respiratory quotient were monitored.Additionally,the changes of gas components,quality,and physiological disorders were assessed for the two cultivars treated with polyethylene (PE) and modified atmosphere packaging (MAP ) after 4 weeks of storage at 0,5,and 10 ℃.[Result ] ‘Lapins’ had lower O 2 consumption rate and CO 2 consumption rate than ‘Regina’ …


Effects Of Ginger Protease On Beef Quality And Protein Degradation, Xinghua CHEN, Shuhong LIU, Huaisheng ZHENG, Zhaobin HUANG, Ping HU 2025 School of Liquor and Food Engineering , Guizhou University , Guiyang 550025 , China

Effects Of Ginger Protease On Beef Quality And Protein Degradation, Xinghua Chen, Shuhong Liu, Huaisheng Zheng, Zhaobin Huang, Ping Hu

Journal of Food Science and Biotechnology

[Objective ] This study aims to investigate the effects of ginger protease on beef tenderness.[Method ] Ginger protease was extracted from mature ginger via ammonium sulfate precipitation.The fundamental properties of the ginger protease,including enzyme activity,protein mass fraction,optimal temperature and pH,and relative molecular mass,were evaluated.Furthermore,the effects of ginger protease on the physicochemical properties,sensory quality,microstructure,and protein degradation characteristics of beef were examined.[Result ] Ginger protease was mainly composed of two proteins with relative molecular masses of 15 293 and 34 191,with a total protein mass fraction of (574.60±0.25) mg/g,an enzyme activity of (31 787±307) U/g,an optimum temperature of 60 ℃,and an optimum …


Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol, Wenjie ZUO, Ping ZHANG, Ruirui XU, Leyi ZHANG, Zhen KANG, Jianghua LI 2025 Science Center for Future Foods , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Jiaxing Institute of Future Food , Jiaxing 314001 , China

Recombinant Pichia Pastoris Fermentation For Biosynthesis Of The Uv Resistant Compound Gadusol, Wenjie Zuo, Ping Zhang, Ruirui Xu, Leyi Zhang, Zhen Kang, Jianghua Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to employ synthetic biology and metabolic engineering techniques to engineer Pichia pastoris (P.pastoris ) for the efficient heterologous synthesis of the UV resistant compound gadusol.[Method ] On the basis of a bioinformatic analysis and codon optimization of the genes involved in the gadusol biosynthetic pathway in Danio rerio,the gadusol fermentation pathway was constructed in P.pastoris via strategies such as promoter engineering,metabolic engineering,and fermentation optimization.[Result ] A high-purity gadusol standard was prepared through optimized extraction and chromatographic separation.The gadusol yield in the recombinant strain P.pastoris HME reached 105 mg/L.By screening of gene expression promoters and optimization …


Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine, Zhenjie LIU, Peihao HUANG, Shu CHEN, Shun RONG, Feiting PENG, Ling CHEN, Weipeng GUO 2025 Institute of Microbiology , Guangdong Academy of Sciences , Guangzhou 510070 , China;Guangdong Detection Center of Microbiology , Guangzhou 510070 , China;State Key Laboratory of Applied Microbiology Southern China , Guangzhou 510070 , China;Guangdong Provincial Key Laboratory of Microbial Safety and Health , Guangzhou 510070 , China;National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food , Guangzhou 510070 , China

Effects Of Critical Environmental Factors On The Antimicrobial Activity Of Ε-Poly- L-Lysine, Zhenjie Liu, Peihao Huang, Shu Chen, Shun Rong, Feiting Peng, Ling Chen, Weipeng Guo

Journal of Food Science and Biotechnology

[Objective ] This study explored the effects of critical environmental factors [water activity (aw),pH,and temperature ] on the antimicrobial activity of ε-poly- L-lysine (ε-PL),aiming to provide a basis for the application of ε-PL.[Method ] With Staphylococcus aureus as an indicator strain,the micro-broth dilution and agar dilution methods as well as antimicrobial growth kinetics were utilized to determine the antimicrobial activity of ε-PL.The protein leakage assay was employed to evaluate the bacterial membrane disruption activity of ε-PL.Live/Dead fluorescence staining was performed to observe the bacterial membrane permeability.[Result ] The minimum inhibitory mass concentration (MIC ) of ε-PL against Staphylococcus aureus was …


Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation, Bo ZHAI, Quanxian SHU, Xiaoyan YANG, Yin MAO, Yu DENG, Yunying ZHAO 2025 School of Biotechnology , Jiangnan University , Wuxi 214122 , China

Probiotic Properties Of Three Strains Of Lactobacillus And Their Application In Corn Husk Fermentation, Bo Zhai, Quanxian Shu, Xiaoyan Yang, Yin Mao, Yu Deng, Yunying Zhao

Journal of Food Science and Biotechnology

[Objective ] This study aims to improve the utilization of maize resources and address the shortage of feed resources.[Method ] The intestinal colonization capacity,tolerance,and antibacterial properties of three Lactobacillus strains,Lactobacillus paracasei ZB- 1 (ZB-1),L.paracasei DY- 2 (DY-2),and L.rhamnosus DY- 4 (DY-4) were evaluated.The most suitable strain for fermenting cornhusks was identified,and its fermentation process was optimized.[Result ] ZB-1 exhibited higher intestinal colonization ability and antibacterial properties than DY- 2 and DY- 4.The antibacterial mechanism of ZB- 1 was likely driven by the action of organic acids in an acidic environment and certain protein-based antimicrobial substances.The fermentation conditions for ZB- 1 …


Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang, Lei SUN, Laifang QI, Qiuyan LIANG, Jinxuan HAN, Tianli FAN, Yuhua MA 2025 College of Chemistry and Chemical Engineering , Xinjiang Normal University , Urumqi 830054 , China;Xinjiang Uygur Autonomous Region Product Quality Supervision and Inspection Research Institute , Urumqi 830011 , China

Diversity Of Fungi On The Surfaces Of Grapes And Raisins In Turpan , Xinjiang, Lei Sun, Laifang Qi, Qiuyan Liang, Jinxuan Han, Tianli Fan, Yuhua Ma

Journal of Food Science and Biotechnology

[Objective ] This study aims to understand the potential harmful fungi that may affect the quality of raisins during the drying process of grapes from Turpan,Xinjiang and to explore the relationships between varieties and the fungal community structure and diversity on the surfaces of grapes and raisins.[Method ] Four popular grape varieties (‘Thompson Seedles s’,‘Boert e’,‘Xiangfe i’,and ‘Seedless Blac k’) in a grapery in Turpan were selected as research objects.The fungal diversity on the surfaces of grapes and raisins was analyzed via high-throughput sequencing.[Result ] The dominant fungal phyla on the surfaces of grapes and raisins were Ascomycota and Basidiomycota.The …


Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model, Zeren CHENG, Xia MENG, Kejing TANG, Ling SHEN, Qing GU, Ping LI 2025 School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China

Regulatory Effects Of Hesperetin And Eriocitrin On Gut Microbiota In An In Vitro Simulation Model, Zeren Cheng, Xia Meng, Kejing Tang, Ling Shen, Qing Gu, Ping Li

Journal of Food Science and Biotechnology

[Objective ] This study aims to compare the effects of hesperetin and eriocitrin on the gut microbiota in different enterotypes.[Method ] An in vitro gut fermentation model was established,which was combined with 16S rRNA high-throughput sequencing and gas chromatography-mass spectrometry (GC-MS ) technique to evaluate the regulatory effects of the two flavonoids on the gut microbiota in different enterotypes.[Result ] After fermentation,both flavonoids significantly reduced the Firmicutes-to-Bacteroidota ratio (F/B ratio ) in terms of relative abundance at the phylum level in enterotype Bacteroides and enterotype Prevotella.At the genus level,pro-inflammatory bacteria including Paeniclostridium and Sutterella showed decreased relative abundance in treatment …


Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen, Xiong HE, Rongxing LI, Shaotong JIANG, Jianfeng LU, Lin LIN 2025 School of Food and Biological Engineering , Hefei University of Technology , Hefei 230601 , China

Effects Of Ultrasound-Assisted Enzymatic Extraction On The Physical And Chemical Properties Of Tortoise Shell Collagen, Xiong He, Rongxing Li, Shaotong Jiang, Jianfeng Lu, Lin Lin

Journal of Food Science and Biotechnology

[Objective ] The effects of different extraction methods on the yield,purity,structural characteristics,and physicochemical properties of tortoise shell collagen were investigated.[Method ] The ultrasound-assisted enzymatic method,acid method,and enzymatic method were used to extract collagen from tortoise shell powder.[Result ] The yield and purity of tortoise shell collagen extracted by ultrasound-assisted enzymatic method were 2.29% and 85.65%,respectively,significantly higher than those extracted by acid method (0.78% and 73.15%) and enzymatic method (1.77% and 75.63%).Ultraviolet absorption spectroscopy,infrared absorption spectroscopy,circular dichroism spectroscopy,and electrophoresis profiles showed that collagens extracted from tortoise shell by three methods were all type I collagen and retained intact triple helix structures.X-ray …


A Novel Turmeric Drying Approach Based On Supercritical Carbon Dioxide, Sumanjot Kaur, Arda Tuhanioglu, Ali Ubeyitogullari 2025 University of Arkansas, Fayetteville

A Novel Turmeric Drying Approach Based On Supercritical Carbon Dioxide, Sumanjot Kaur, Arda Tuhanioglu, Ali Ubeyitogullari

Food Science Faculty Publications and Presentations

As a widely used spice, turmeric is rich in curcuminoids, which are known for their anti-inflammatory and antioxidant properties. However, the drying process, especially at high temperatures, often leads to significant curcumin degradation, impacting its bioactivity and quality. This study investigates drying turmeric using an innovative approach based on supercritical carbon dioxide (SC-CO2), and conventional drying methods (oven drying and freeze-drying) in terms of curcuminoid retention, color, solubility, chemical interactions, crystallinity, and morphology. The results revealed that SC-CO2 drying preserved up to 82.3 mu g curcuminoids /mg dried turmeric, which was significantly higher than the levels retained by oven drying …


In Vitro Screening Of Antibacterial Efficacy Of Moringa Oleifera And Thymus Vulgaris Methanolic Extracts Against Different Escherichia Coli Strains And Their In Vivo Effects Against E. Coli-Induced Infection In Broiler Chickens, Majid Ali, Naila Chand, Sarzamin Khan, Rifat Ullah Khan, Babar Maqbool, Shabana Naz, Ala Abudabos, Abdul Hafeez, Ibrahim A. Alhidary 2025 The University of Agriculture, Peshawar 25130, Pakistan

In Vitro Screening Of Antibacterial Efficacy Of Moringa Oleifera And Thymus Vulgaris Methanolic Extracts Against Different Escherichia Coli Strains And Their In Vivo Effects Against E. Coli-Induced Infection In Broiler Chickens, Majid Ali, Naila Chand, Sarzamin Khan, Rifat Ullah Khan, Babar Maqbool, Shabana Naz, Ala Abudabos, Abdul Hafeez, Ibrahim A. Alhidary

Food and Animal Sciences Faculty Research

This study evaluated the antibacterial efficacy and growth-promoting potential of Moringa oleifera and Thymus vulgaris methanolic extracts in broiler chickens challenged with Escherichia coli O78. In vitro antibacterial screening using agar well diffusion and disc diffusion assays revealed that ciprofloxacin exhibited the strongest inhibitory effect, followed by tetracycline and enrofloxacin, whereas among plant extracts, T. vulgaris was more effective than M. oleifera. The optimal combination (M100T150; 100 mg M. oleifera + 150 mg T. vulgaris) produced the largest inhibition zones against E. coli strains. For the in vivo trial, 540 Ross-308 broiler chicks were distributed into six treatment groups in …


Harnessing Rice Glutelin And Chickpea Proteins Interactions To Enhance Functional And Nutritional Qualities Of Plant-Based Cheese, Nikitha Modupalli, Md Mahfuzur Rahman 2025 University of Arkansas, Fayetteville

Harnessing Rice Glutelin And Chickpea Proteins Interactions To Enhance Functional And Nutritional Qualities Of Plant-Based Cheese, Nikitha Modupalli, Md Mahfuzur Rahman

Food Science Faculty Publications and Presentations

The recent demand for plant-based dairy alternatives has increased the focus on improving the organoleptic, and nutritional attributes of plant cheeses to match their dairy counterparts. This study aimed to develop a functional cheese using the synergistic interactions of rice glutelin and chickpea proteins to enhance both nutritional and textural properties. Functional characterization revealed that rice glutelin exhibited a higher water-holding capacity of 340 % and elevated solubility, while chickpea protein showed a superior oil-holding capacity of 550 % and higher surface hydrophobicity. Cheeses were prepared using a 40:60 blend of rice glutelin to chickpea protein, which yielded 21.3 % …


Growing South Dakota (Fall 2025), College of Agriculture, Food and Environmental Sciences 2025 South Dakota State University

Growing South Dakota (Fall 2025), College Of Agriculture, Food And Environmental Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Mesonet at SDState: A benefit to every South Dakotan
[Page] 6 Governor’s Ag Ambassador — Glenn Muller
[Page] 8 Precision agriculture cooperative agreement signed with University of Debrecen
[Page] 10 Students experience international agriculture, culture in Vietnam, New Zealand
[Page] 12 Legen-dairy! SDSU students claim national judging awards
[Page] 13 SDSU Rodeo Team continues storied rodeo tradition at nationals
[Page] 14 SDSU wheat breeders helping feed the world
[Page] 20 SDSU drone holds promise for soil, leaf sampling
[Page] 22 Don't be a fish out of water
[Page] 24 A passion for academic advising
[Page] 25 One takeaway …


Physicochemical And Structural Optimization Of Date (Phoenix Dactylifera L.) Syrup: Impact Of Extraction Temperatures And Pectin Addition, Afna Thaivalappil Abdul Asiz 2025 United Arab Emirates University

Physicochemical And Structural Optimization Of Date (Phoenix Dactylifera L.) Syrup: Impact Of Extraction Temperatures And Pectin Addition, Afna Thaivalappil Abdul Asiz

Theses

This thesis focuses on the optimization of date (Phoenix dactylifera L.) syrup, an emerging natural sweetener rich in glucose, fructose, and bioactive compounds, by investigating how extraction temperature and pectin incorporation influence its physicochemical and structural properties. Therefore, the main objective of this study was to examine how extraction temperatures (25°C, 50°C, 75°C, and 90°C), and the incorporation of lowmethoxyl pectin (LMP, DE 31%) and high-methoxyl pectin (HMP, DE 85%) prior to concentration, affect the quality, stability, and functionality of date syrup. The results showed that increasing extraction temperatures increased the total soluble solids (16 °Brix at 25°C to …


Machine Learning-Based Optimization Of Alginate, Guar Gum, And Pectin-Based Edible Coatings For Extended Strawberry Shelf Life, Saklain Niam, Iftekhar Ahmad, Md Abu Rayhan, Sajid Mahmood, Parvej Hasan Jon, MD Monir Ahmed 2025 Shahjalal Univ Sci & Technol

Machine Learning-Based Optimization Of Alginate, Guar Gum, And Pectin-Based Edible Coatings For Extended Strawberry Shelf Life, Saklain Niam, Iftekhar Ahmad, Md Abu Rayhan, Sajid Mahmood, Parvej Hasan Jon, Md Monir Ahmed

Food Science Faculty Publications and Presentations

Strawberries deteriorate rapidly under warm, humid conditions, and to make things worse, in many tropical settings refrigerated logistics are limited. Edible biopolymer coatings offer a low-cost method to slow quality loss, but multicomponent formulations behave nonlinearly. In this study, ten types of coatings were developed and evaluated for their effects on the physicochemical (weight loss, firmness, total soluble solids (TSS), pH, color change (Delta E)) and biochemical properties (total phenolic content (TPC), antioxidant property (DPPH) of strawberries over a 6-day storage period at 25 degrees C (298.15K). The optimal formulation, consisting of 0.56 % (w/v) alginate, 0.33 % (w/v) guar …


The Green Plate Effect: Systematic Review And Meta-Analyses Of Vegan Diets And Metabolic Health In Adults- Findings From Randomized Controlled Trials, Wai-kit Tom, Aaron Deming Looi, Vijayakumar Nithusharini, Kavitha Lakshmipathy, Uma Devi Palanisamy, Usha Sundralingam 2025 Monash University

The Green Plate Effect: Systematic Review And Meta-Analyses Of Vegan Diets And Metabolic Health In Adults- Findings From Randomized Controlled Trials, Wai-Kit Tom, Aaron Deming Looi, Vijayakumar Nithusharini, Kavitha Lakshmipathy, Uma Devi Palanisamy, Usha Sundralingam

Food Science Faculty Publications and Presentations

Background: The increasing global interest in consuming plant-based diets, especially the vegan diet, is fuelled by ethical, health, and environmental concerns. A balanced and diverse vegan diet has the potential to play a significant role in achieving and sustaining optimal health, particularly in diet-based interventions for metabolic syndrome. Scope and approach: This systematic review evaluated randomised controlled trials, to elucidate the effects of a strict vegan diet on individual components of metabolic syndrome in the human population. A comprehensive literature search was conducted across four databases (Cochrane Central Register of Controlled Trials; Embase via Ovid; Ovid Medline; Scopus). The search …


Unraveling Crop Nitrogen-Water Dynamics With Hyperspectral-Thermal Sensing In Northern Central Valley, California, Shahryar Fazli, Surendra Maharjan, Wenzhao Li, Joshua B. Fisher, Rejoice Thomas, Fernando Romero Galvan, Gabriela Shirkey, Hesham el-Askary 2025 Chapman University

Unraveling Crop Nitrogen-Water Dynamics With Hyperspectral-Thermal Sensing In Northern Central Valley, California, Shahryar Fazli, Surendra Maharjan, Wenzhao Li, Joshua B. Fisher, Rejoice Thomas, Fernando Romero Galvan, Gabriela Shirkey, Hesham El-Askary

Mathematics, Physics, and Computer Science Faculty Articles and Research

Ensuring global food security in the face of climate change requires optimizing crop water use and nutrient management. This study investigates the relationship between canopy nitrogen (N) and evapotranspiration (ET) across sunflower, rice, walnut, alfalfa, and plum crops using advanced remote sensing technologies. High-resolution hyperspectral data from NASAs Earth Surface Mineral Dust Source Investigation (EMIT) and thermal multispectral data from the Landsat-based OpenET system were analyzed over 1,135 km2 in California. Regression analysis revealed strong spatial association between canopy N and ET for sunflower (R2 = 0.82), rice (R2 = 0.71), and walnut (R2 = 0.68), …


Detection Method For Escherichia Coli Using Real-Time Polymerase Chain Reaction Targeting The Yhav Gene, Muktiningsih Nurjayadi, Anisa Fitriyanti, Royna Rahma Musie, Gusti Angieta Putri, Puan Aqila Azizah, Helzi Angelina, Grace Grace, Ananda Indah Putri Sihombing, Agus Setiawan, Jefferson Lynford Declan, Gladys Indira Putri, Dandy Akbar Juliansyah, Siti Fatimah, Ayu Berkahingrum, Irma Ratna Kartika, Fera Kurniadewi, Vira Saamia, Shyi-Tien Chen, Bassam Aboemolak, Hesham Ali El Enshasy 2025 Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Negeri Jakarta, Jakarta Timur 13220, Indonesia

Detection Method For Escherichia Coli Using Real-Time Polymerase Chain Reaction Targeting The Yhav Gene, Muktiningsih Nurjayadi, Anisa Fitriyanti, Royna Rahma Musie, Gusti Angieta Putri, Puan Aqila Azizah, Helzi Angelina, Grace Grace, Ananda Indah Putri Sihombing, Agus Setiawan, Jefferson Lynford Declan, Gladys Indira Putri, Dandy Akbar Juliansyah, Siti Fatimah, Ayu Berkahingrum, Irma Ratna Kartika, Fera Kurniadewi, Vira Saamia, Shyi-Tien Chen, Bassam Aboemolak, Hesham Ali El Enshasy

Makara Journal of Science

Escherichia coli is a foodborne pathogenic bacterium that can cause diarrhea, while yhaV is a virulence-associated gene linked to the toxin–antitoxin system in E. coli. This study was aimed at evaluating the confirmation, specificity, and sensitivity of a yhaV gene primer using real-time polymerase chain reaction. The yhaV-targeting PCR successfully amplified a DNA fragment with an amplicon length of 207 bp (base pairs) under an annealing temperature optimized to a range of 54 °C to 62 °C via gradient PCR. The PCR using the primer pair produced a consistent Ct (cycle threshold) of 14.14 ± 0.05 and showed …


Organic Glycinate Trace Minerals Improve Hatchability, Bone And Eggshell Breaking Strength, And Mineral Uptake During Late Laying Cycle In Layer Breeders, Mujtaba Akram Jahangir, Muhammad Muneeb, Muhammad Farooq Iqbal, Syeda Maryam Hussain, Syed Sohail Habib, Sohail Ahmad, Kasim Sakran Abass, Nasir Mukhtar, Rashed A. Alhotan, Ali R. Al Sulaiman, Ala E. Abudabos 2025 PMAS Arid Agriculture University

Organic Glycinate Trace Minerals Improve Hatchability, Bone And Eggshell Breaking Strength, And Mineral Uptake During Late Laying Cycle In Layer Breeders, Mujtaba Akram Jahangir, Muhammad Muneeb, Muhammad Farooq Iqbal, Syeda Maryam Hussain, Syed Sohail Habib, Sohail Ahmad, Kasim Sakran Abass, Nasir Mukhtar, Rashed A. Alhotan, Ali R. Al Sulaiman, Ala E. Abudabos

Food and Animal Sciences Faculty Research

This study evaluated the effects of replacing inorganic trace minerals with organic glycinate forms at two inclusion levels in layer breeders during the late laying cycle over 6 weeks (66 to 72 weeks of age). For this, a total of 180 layer breeders (162 hens and 18 males; Lohmann LSL Ultralite) were randomly divided into three treatment groups having six replicates of nine hens each and one male for each replicate following a completely randomized design (CRD). The dietary treatments included: (1) Basal diet + inorganic trace minerals at breed recommended levels (ITM100), (2) Basal diet + organic trace minerals …


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