Application Of A Rapid Test Strip Method In Quantitative Detection Of Microorganisms In Chilled Meat,
2025
College of Life Sciences , Linyi University , Linyi 276005 , China;Department of Biotechnology , The University of Suwon , Suwon 18323 , South Korea
Application Of A Rapid Test Strip Method In Quantitative Detection Of Microorganisms In Chilled Meat, Yanzhen Zhang, Ning Liu, Wei Wang, Xianqi Yao, Laixue Ni, Yunguo Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the feasibility of using the rapid test strip method for detecting key microbial quality control indicators in chilled meat,specifically the total plate count and Escherichia coli /coliforms.[Method ] The performance of the rapid test strip method was assessed through repeatability tests,and the national standard plate count method was used as the reference for method comparison.Statistical significance of the quantification results was analyzed by a t-test,and linear regression was adopted to analyze the correlation.[Result ] The repeatability deviation of the rapid test strip method (r≤0.25) met the requirements of ISO 4833—1:2013.No significant difference was …
Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey,
2025
School of Liquor and Food Engineering , Guizhou University , Guiyang 550025 , China ;Guizhou Xijiu Investment Holding Group Co ., Ltd ., Zunyi 564662 , China
Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey, Taotao Wei, Qian Chen, Xiangjie Tao, Bin Tang, Fusheng Ren, Ping Hu
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the impacts of single strain fermentation and mixed fermentation on the flavor,composition,and antioxidant activity of soybean whey.[Method ] Gas chromatography-ion mobility spectrometry (GC-IMS ) was coupled with E-nose and high-performance liquid chromatography (HPLC ) to a nalyze the flavor and function of soybean whey fermented by the single and mixed strains.[Result ] Compared with single-strain fermentation by Limosilactobacillus fermentum HJS- 1 or Kluyveromyces marxianus HJS- 2,mixed fermentation (Limosilactobacillus fermentum HJS- 1 + Kluyveromyces marxianus HJS- 2) effectively improved the levels of functional substances and antioxidant activity of soybean whey.Compared with non-fermented soybean whey,mixed …
Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli,
2025
State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China
Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli, Luxi Gan, Heng Gong
Journal of Food Science and Biotechnology
[Objective ] This study aimed to improve the yield and conversion rate of 5-methylpyrazine- 2-carboxylic acid (MPCA ) through biocatalysis.[Method ] Escherichia coli (E.coli）was used as the model organism to construct an MPCA synthesis system,and the whole-cell catalytic reaction conditions of recombinant E.coli were optimized.In addition,the parental host strain was subjected to ultraviolet mutagenesis for the screening of host strains tolerant to high mass concentrations of 2,5-dimethylpyrazine (DMP ).[Result ] The recombinant mutagenized strain could completely convert 10 g/L DMP to MPCA within 12 h in a 10 mL reaction system,achieving a yield of 12.9 g/L and a corresponding molar …
Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation,
2025
School of Food Engineering , Harbin University , Harbin 150086 , China
Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- …
Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein,
2025
College of Life Sciences and Medicine , Zhejiang Sci-Tech University , Hangzhou 310018 , China;Shaoxing Academy of Biomedicine , Zhejiang Sci-Tech University , Shaoxing 312369 , China
Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein, Zhuo Jiang, Weina Lu, Qi Lin, Zhan Luo, Zhengbin Lyu, Zuoming Nie
Journal of Food Science and Biotechnology
[Objective ] This study aims to design a fusion protein with high activities and high stability of superoxide dismutase (SOD ) and catalase (CAT ).[Method ] Using AcSOD 11 and FeCAT proteins as the research subjects,three fusion proteins of SOD and CAT were designed:AcSOD 11-(GGGGS )*3-FeCAT,FeCAT-(GGGGS )*3-AcSOD 11,and FeCAT- (EAAAK )*3-AcSOD 11,which were named AcSOD 11-G3-FeCAT,FeCAT-G3-AcSOD 11,and FeCAT-E 3-AcSOD 11,respectively.Soluble expression of these three fusion proteins was achieved in Escherichia coli BL21(DE3).The thermal stability and pH stability of the three fusion proteins were then assessed.[Result ] The three fusion proteins showed no significant difference in the specific activity of SOD,while …
Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications,
2025
University of Connecticut - Storrs
Fabrication Of Three-Dimensional Seaweed-Based Scaffolds And Evaluation Of Cell Attachment And Viability For Cultured Meat Applications, Srishti Tandon
Honors Scholar Theses
Global demand for animal protein is increasing rapidly, underscoring the need for sustainable meat production. Cultured meat is a promising alternative to traditional meat production techniques, but it requires optimization before it can be implemented on a large scale. Scaffolds are an important component for making cultured meat, as they provide structural support for cell attachment and mimic the texture of meat. Scaffolds need to be biocompatible, mechanically supportive, and biochemically active to promote proper cell adhesion, proliferation, and differentiation. This project investigated brown seaweed extract (SWE) hydrogels as a scaffold for cultivated meat applications and compared their mechanical and …
Fruit Characteristics Of F1 Nil Sletr1-2 Vary With Parental Background,
2025
Master Program of Agronomy, Faculty of Agriculture, Universitas Padjadjaran
Fruit Characteristics Of F1 Nil Sletr1-2 Vary With Parental Background, Imas Mintarsih, Anne Nuraini, Farida, Erni Suminar, Rika Meliansyah, Syariful Mubarok
Jurnal Kultivasi
The Sletr1-2 allele, which reduces ethylene sensitivity in tomatoes, offers a promising approach to extending fruit shelf life by slowing ripening-related metabolism. However, the trait’s success depends on compatibility with the recipient cultivar’s genetic background. This study evaluated fruit characteristics of F1 near-isogenic line (NIL) hybrids carrying Sletr1-2 in the commercial backgrounds ‘Costoluto’, ‘Oxheart’, and ‘Sart Roloise’. The field trial was carried out between May and August 2024 at the Experimental Station of Universitas Padjadjaran, Sumedang, Indonesia, following a randomized complete block design with eight biological replicates for each treatment. The results revealed that fruit characteristics of F1 NIL Sletr1-2 …
Reducing Foodborne Bacteria Public Health Burden By Novel Compounds And Pressure-Based Technologies,
2025
Tennessee State University
Reducing Foodborne Bacteria Public Health Burden By Novel Compounds And Pressure-Based Technologies, Junice Alannah Sibley
Tennessee State University Alumni Theses and Dissertations
Food safety strategies are being developed in the hopes of combating foodborne pathogens and their risks. Previous techniques and technologies are struggling to meet the needs of the public, leading to the demand for new, innovative methods. Mild high-pressure processing (HPP) of 350 MPa was used in a synergetic combination with mild heat of 60°C and the antimicrobial carvacrol for the decontamination of non-typhoidal Salmonella serovars that resulted in a >5 log CFU/mL reduction. Additionally, a secondary study was conducted comparing a single Salmonella Tennessee strain with a 4-strain Salmonella cocktail to examine sensitivity to pressure-based treatments resulting in a …
Acid And Sequential Acid–Alkali Extraction To Mitigate Enzyme Activity And Off-Flavors In Chickpea Protein: Implications On Structural, Functional, And Bioactive Properties,
2025
University of Arkansas, Fayetteville
Acid And Sequential Acid–Alkali Extraction To Mitigate Enzyme Activity And Off-Flavors In Chickpea Protein: Implications On Structural, Functional, And Bioactive Properties, Kavitha Lakshmipathy, Sukanya Poddar, Nikitha Modupalli, Scott Lafontaine, Md Mahfuzur Rahman
Food Science Faculty Publications and Presentations
Chickpea proteins offer significant nutritional and functional benefits in food products; however, their utilization is often restricted by off-flavors, primarily for lipoxygenase (LOX) and lipase activity. This study aims to develop a novel extraction method by combining acid (pH 2.0) extraction with the conventional alkali (pH 9.0) method to inactivate enzymes and enhance protein quality. Acid-extracted proteins exhibit the highest purity (83.4%), albumin content, and structural flexibility (46.65% random coil). Both acid and sequential acid-alkali-extracted proteins significantly reduced LOX (0.75 and 0.88 U/g) and lipase (0.10 and 0.08 U/g) activity. Acid extraction significantly lowers volatile compounds such as hexanal (0.0409 …
Exploring The Autofermented Cyanobacterial Crude Extract As Antimicrobial Animal Feed Additives.,
2025
Tennessee State University
Exploring The Autofermented Cyanobacterial Crude Extract As Antimicrobial Animal Feed Additives., Mohana Krishnan Neelakrishnan
Tennessee State University Alumni Theses and Dissertations
Overexploitation of antibiotics in the animal industry has driven the need for reduced usage, but this reduction has intensified the challenge posed by Salmonella enterica and Campylobacter jejuni to livestock systems and public health. This situation underlines the need for sustainable antimicrobial alternatives. Although cyanobacteria are promising in this respect, since they may produce a variety of bioactive metabolites, the amounts of these metabolites in their natural state are insufficient to elicit potent antibacterial action. In this study, autofermentation was investigated as a means of enhancing antibacterial efficacy in Fischerella ambigua and Nostoc muscorum by promoting the synthesis of organic …
Dietary Supplementation In Lambs: Evaluating The Effects Of Depth Charge On Meat Composition,
2025
Lipscomb University
Dietary Supplementation In Lambs: Evaluating The Effects Of Depth Charge On Meat Composition, Alianna Koesel
Student Scholar Symposium
Often, animals destined for consumption are fed supplements to help speed up or slow down their growth rate, enhance aesthetics, or standardize the product. Herein, we examined the supplement Depth Charge and its effects on the fat and protein content in lambs of the same age and lineage. Depth Charge increases fiber consumption to add spring of rib or an outward rib cage curvature to an animal's appearance while restricting feed consumption. Since the supplement targets the rib-loin area, we chose to analyze loin chop, loin roast, and hamburger cuts from each lamb and conducted experiments to determine how each …
Sabbatical Visit To Micro4food Research Group At The Free University Of Bolzano,
2025
Utah State University
Sabbatical Visit To Micro4food Research Group At The Free University Of Bolzano, Robert Ward
Funded Research Records
No abstract provided.
Effect Of Thermodynamic Parameters And Intrinsic Properties On Crystallization Kinetics And Physical Properties Of Fats,
2025
Utah State University
Effect Of Thermodynamic Parameters And Intrinsic Properties On Crystallization Kinetics And Physical Properties Of Fats, Silvana Martini
Funded Research Records
No abstract provided.
Elucidating Mechanism Of Formation Of Thermoreversible Gels From Associative Protein-Polysaccharide Mixtures,
2025
Utah State University
Elucidating Mechanism Of Formation Of Thermoreversible Gels From Associative Protein-Polysaccharide Mixtures, Prateek Sharma
Funded Research Records
No abstract provided.
Uv-A Light Activated Biodegradable Antimicrobial Packaging: A Sustainable Hurdle Technology For Food Preservation,
2025
Utah State University
Uv-A Light Activated Biodegradable Antimicrobial Packaging: A Sustainable Hurdle Technology For Food Preservation, Luis J. Bastarrachea
Funded Research Records
No abstract provided.
Investigating The Role Of Mitochondria In Postmortem Metabolism And Shelf Stability Of Fresh Meat,
2025
Utah State University
Investigating The Role Of Mitochondria In Postmortem Metabolism And Shelf Stability Of Fresh Meat, Sulaiman K. Matarneh, Kara J. Thornton-Kurth, Stephan Van Vliet
Funded Research Records
No abstract provided.
Automated Detection Of Kinky Back In Broiler Chickens Using Optimized Deep Learning Techniques,
2025
University of Arkansas, Fayetteville
Automated Detection Of Kinky Back In Broiler Chickens Using Optimized Deep Learning Techniques, Ramesh Bahadur Bist, Andi Asnayanti, Anh Dang Trieu Do, Yang Tian, Chaitanya Palleria, Dongyi Wang, Adnan Alrubaye
Food Science Faculty Publications and Presentations
The global poultry industry faces growing challenges from skeletal disorders, with Kinky Back (KB) significantly impacting broiler welfare and production. KB causes spinal deformities that reduce mobility, feed access, and increase mortality. It often remains undetected in early subclinical stages. Traditional KB diagnosis methods are slow and subjective, and highlighting the need for an automated and objective detection. This study develops a machine learning approach for detecting KB in broilers using image data. Male Cobb 500 broilers were raised under controlled conditions and monitored over 7 weeks using overhead 4K video cameras. Behavioral and posture data related to KB were …
Assessment Of Microbiological And Physicochemical Parameters In Ground Beef From Fast Food Outlets In The Prishtina Region,
2025
University for Business and Technology-UBT
Assessment Of Microbiological And Physicochemical Parameters In Ground Beef From Fast Food Outlets In The Prishtina Region, Diora Kastrati, Veton Haziri, Ibrahim Mehmeti, Violeta Lajqi Makolli, Arianeta Nura, Flutura C. Ajazi
International Journal of Business and Technology
Fast food consumption has significantly increased, particularly in fast-paced cities like Prishtina. Hamburgers, one of the most consumed products in this industry, rely on ground beef, which poses notable food safety risks due to potential contamination and inadequate hygiene practices. The aim of this study was to evaluate selected microbiological and physicochemical parameters in ground beef from fast food outlets in the Prishtina region. The research involved analyzing 12 samples from various fast-food establishments in Prishtina, collected during June-July 2024. Microbiological analyses focused on total mesophilic aerobic bacteria and Escherichia coli, using standard cultivation methods on Plate Count Agar and …
Development And Evaluation Of Rhizoqol, A Quality-Of-Life Caregiver-Reported Survey For Rhizomelic Chondrodysplasia Punctata, A Rare Peroxisomal Disorder,
2025
Montclair State University
Development And Evaluation Of Rhizoqol, A Quality-Of-Life Caregiver-Reported Survey For Rhizomelic Chondrodysplasia Punctata, A Rare Peroxisomal Disorder, Mousumi Bose, Tahra C. Anglade, Chelsea I. Donlon, Adrian L. Kerrihard, Hila F. Berger, Ariel S. Berkowitz, Shawn A. Ritchie, Tara M. Smith
Department of Nutrition and Food Studies Scholarship and Creative Works
Background: Rhizomelic chondrodysplasia punctata (RCDP) is a rare genetic disorder characterized by symptoms such as respiratory dysfunction, seizures, orthopedic issues, and neurodevelopmental delay. Potential therapeutics for RCDP warrant the development of clinical outcome assessments to assess the efficacy of treatment and the well-being of patients. Our study aimed to develop a valid quality-of-life (QOL) caregiver-reported survey instrument, RhizoQOL, to be used as a supportive endpoint in RCDP clinical trials. Methods: Development of the RhizoQOL survey tool included three RCDP caregiver focus groups to elicit concepts to serve as potential domains in a QOL survey instrument for RCDP, pilot survey development …
Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies,
2025
University of Nebraska-Lincoln
Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies, Xu Shi
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Heart failure (HF) is a leading cause of morbidity and mortality worldwide, placing substantial clinical and economic burdens on society. Although advances in therapy have improved patient outcomes, the biological mechanisms that drive HF onset and progression remain incompletely understood. Increasing evidence suggests that the gut microbiota, the trillions of microorganisms residing in the gastrointestinal tract, plays a key role in some forms of heart disease, especially cardiovascular disease. Moreover, gut microbial dysbiosis has been associated with systemic inflammation, metabolic imbalance, and immune dysregulation, all of which contribute to the pathophysiology of HF. Cardiomyopathy, a primary disorder of the heart …
