Extraction Process Optimization And Antioxidant And Blood Glucose-Lowering Effect Evaluation Of Polyphenols From Camellia Japonica Flowers,
2025
School of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464399 , China ;Henan Engineering Technology Research Center for Comprehensive Utilization of Characteristic Food Resources in Dabie Mountains , Xinyang 464399 , China
Extraction Process Optimization And Antioxidant And Blood Glucose-Lowering Effect Evaluation Of Polyphenols From Camellia Japonica Flowers, Long Chen, Xiaocong Yang, Shujie Xing, Xinyao Shi, Xueke Zheng, Jing Zhu
Journal of Food Science and Biotechnology
[Objective ] This study optimized the extraction process of polyphenols from Camellia japonica (C.japonica ) flowers and preliminarily explored the antioxidant and blood glucose-lowering effects of the extracted polyphenols,aiming to improve the utilization of C.japonica flowers.[Method ] With the flowers of Xinyang Maojian as raw materials,the ultrasonic-assisted method was adopted to extract polyphenols from C.japonica flowers,and the antioxidant and blood glucose-lowering activities of the extracted polyphenols were analyzed.With the extraction rate of polyphenols from C.japonica flowers as the evaluation index,the response surface methodology was adopted to explore the effects of ethanol volume fraction,solid-liquid ratio,extraction time,and ultrasonic power on the extraction …
Site-Directed Mutagenesis And Covalent Organic Framework Immobilization Of Serine Carboxypeptidase Y From Aspergillus Oryzae M30011 For Synergistic Enhancement Of Enzyme Performance,
2025
Beijing Laboratory for Food Quality and Safety , Beijing Technology and Business University , Beijing 100048 , China
Site-Directed Mutagenesis And Covalent Organic Framework Immobilization Of Serine Carboxypeptidase Y From Aspergillus Oryzae M30011 For Synergistic Enhancement Of Enzyme Performance, Jiawen Jiang, Bin Cai, Ke Xiong, Shucan Mao, Yiqiang Chen, Songlin Li, Pengcheng Zhang, Wei Yang, Borui Li
Journal of Food Science and Biotechnology
[Objective ] To enhance the stability of serine carboxypeptidase during enzymatic hydrolysis and expand its industrial applicability,the author employed rational design and immobilization technology to develop efficient and stable enzymatic catalysts in view of the easy deactivation of this enzyme.[Method ] On the basis of the serine carboxypeptidase Y (CPY ) derived from Aspergillus oryzae M30011,single mutant enzymes M 517R,I464R,and Y 271R and the triple mutant enzyme M 517R/I464R/Y271R were designed.These mutant enzymes were then prokaryotically expressed in Escherichia coli and subjected to enzyme activity assays.Subsequently,the mutant enzyme with the highest activity was immobilized with covalent organic framework materials (COFs …
Inhibition Mechanisms Of Naringenin And Egcg Against The Formation Of The Heterocyclic Amine Phip,
2025
Key Laboratory of Geriatric Nutrition and Health , Ministry of Education , Beijing Technology and Business University , Beijing 100048 , China
Inhibition Mechanisms Of Naringenin And Egcg Against The Formation Of The Heterocyclic Amine Phip, Shuai Yu, Qingchen Luo, Mengmeng Feng, Xin Ai, Yubin Li, Liang Zhao
Journal of Food Science and Biotechnology
[Objective ] This study clarifies the key roles of naringenin and epigallocatechin gallate (EGCG ) in inhibiting 2-amino- 1-methyl- 6-phenylimidazo [4,5-b]pyridine (PhlP ) formation in roasted meat and reveals the molecular mechanisms by which naringenin and EGCG regulate precursor consumption and intermediate accumulation,aiming to provide a scientific basis for reducing the risk of heterocyclic amines in food processing.[Method ] The changes in precursors of PhIP in roasted chicken breast were determined,and a model reaction system of flavonoids,methylglyoxal (MGO ),and phenylalanine (Phe) was established.The reaction kinetics curves of key substances were studied to decipher the inhibition mechanisms of naringenin and EGCG …
Co-Expression And Application Of Enzymes For Preparation Of Β-Nicotinamide Mononucleotide,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China;State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Co-Expression And Application Of Enzymes For Preparation Of Β-Nicotinamide Mononucleotide, Jiehu Liu, Lingqia Su, Huixian Fu, Jing Wu
Journal of Food Science and Biotechnology
[Objective ] This study aims to reduce the cost and simplify the production process of β-nicotinamide mononucleotide (β-NMN ) by the enzyme catalytic method.[Method ] On the basis of the previously established β-NMN synthesis system with six enzymes,the six enzyme genes were combined in pairs to construct double-enzyme co-expression recombinant strains.Each crude enzyme solution obtained by co-expression was used for multi-enzyme catalytic cascade reaction to synthesize β-NMN.[Result ] The yield of β-NMN was as high as 72.90% when the concentration of the substrate D-ribose was 10 mmol/L.The optimal temperature and IPTG induction concentration for inducing the enzyme expression of the …
Effects Of Exogenous Enzyme Treatment On Quality And Main Components Of Summer Green Tea,
2025
Tea Research Institute , Chongqing Academy of Agricultural Sciences , Chongqing 402160 , China
Effects Of Exogenous Enzyme Treatment On Quality And Main Components Of Summer Green Tea, Shanmin Chen, Hongyu Luo, Yi Wang, Rui Chang, Linying Yuan, Juan Yang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effect of exogenous enzyme treatment on the quality of summer green tea and optimize the enzyme treatment conditions.[Method ] Sensory evaluation was employed to evaluate and screen the tea samples treated with six exogenous enzymes.The effects of cellulase,tannase,and treatment conditions on the sensory quality,bitter/astringent taste attributes,and main biochemical components of summer green tea were studied by electronic tongue and chemometrics methods.The optimal treatment conditions of cellulase and tannase were comprehensively evaluated by hierarchical cluster analysis,correlation analysis,and principal component analysis (PCA ).[Result ] Sensory evaluation revealed that cellulase and tannase significantly improved the …
Comparative Impacts Of Irradiation On Antioxidant Compounds Of Scalded And Non-Scalded Skin Of Granny Smith Apples,
2025
Chapman University
Comparative Impacts Of Irradiation On Antioxidant Compounds Of Scalded And Non-Scalded Skin Of Granny Smith Apples, Angela Banda
Food Science (MS) Theses
Post-harvest Superficial scald affects the quality of Granny Smith Apples. Oxidative stress develops superficial scald with antioxidant systems mitigating the extent of this damage. This study evaluated the effects of gamma, X-ray, and electron beam irradiation on antioxidants – ascorbic acid, total phenolics, α-tocopherol, carotenoids, chlorophyll, and campesterol – in scalded and non-scalded apple peel tissue. Apples were treated with 0.2-0.77 kGy and stored at 0-1 °C. Irradiation at the applied doses increased scald incidence, suggesting that disruption of antioxidant systems promoted reactive oxygen species accumulation and membrane damage. While certain antioxidants appeared to be upregulated as a stress response, …
Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library,
2025
State Key Laboratory of Food Science and Resources , Nanchang University , Nanchang 330047 , China;College of Food Science & Technology , Nanchang University , Nanchang 330047 , China
Preparation Of Human Asprosin Nanobodies Based On A Natural Nanobody Phage Display Library, Yun Liang, Yi Huang, Qinghua He
Journal of Food Science and Biotechnology
[Objective ] Asprosin (ASP ),an adipokine produced from fibrillin- 1 (FBN 1) cleavage,is closely linked to type 2 diabetes mellitus and obesity when its level is abnormal,demonstrating significant effect on human health.Therefore,monitoring the ASP level is crucial.The author aims to screen the nanobodies specifically binding to ASP via phage display technology,offering a new antibody component for the research on ASP.[Method ] The antigenic epitopes of ASP were predicted by the online tools ABCpred and NovoPro,and then coupled with bovine serum albumin (BSA ) to form the coating antigen (ASP-Epitope-BSA ).After five rounds of bio-panning,the nanobodies specifically binding to ASP were …
Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships,
2025
School of Chemical and Material Engineering , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Synthetic and Biological Colloids , Ministry of Education , Jiangnan University , Wuxi 214122 , China
Modulatory Effects Of Flavonoids On Il- 4/Il-13 In Atopic Dermatitis And Their Structure-Activity Relationships, Yueyuan Wang, Jing Liu, Chen Li, Fengfeng Chen, Cheng Yang, Bingtian Zhao
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the ameliorating effects of flavonoids on atopic dermatitis (AD) and their structure-activity relationships.[Method ] Flavonoids were selected through network pharmacology with interleukin- 4 (IL-4) and interleukin- 13 (IL-13) as key targets.The immunoglobulin E (IgE)-induced RBL- 2H3 cell model was employed to evaluate the effects of flavonoids on inflammatory factors,degranulation activities,and calcium ion influx,and the structure-activity relationships were analyzed.Finally,molecular docking was utilized to assist in exploring the action mechanism.[Result ] All of the six flavonoids (luteolin,apigenin,chrysin,quercetin,kaempferol,and fisetin ) screened by network pharmacology had significant inhibitory effects on the expression of IL- 4,IL- 13,tumor necrosis …
Structural And Solution Property Characterization Of Oat Β-Glucans,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Structural And Solution Property Characterization Of Oat Β-Glucans, Hongli Zhang, Yaning Shi, Ming Miao
Journal of Food Science and Biotechnology
[Objective ] This study aims to characterize the structure and investigate the solution properties of oat β-glucan (OBG ).[Method ] Two soluble OBGs with different weigh-average molecular weights (Mw),number-average molar weights (Mn),and peak molecular weights (Mp) were prepared by hot water extraction and ethanol classification.The structures and solution properties were characterized by Fourier-transform infrared spectroscopy (FT-IR ),nuclear magnetic resonance (NMR ),X-ray diffraction (XRD ),scanning electron microscopy (SEM ),high performance size exclusion chromatography coupled with multi-angle laser light scattering (HPSEC-MALLS ),and rheology.[Result ] OBG- 1 and OBG- 2 showed the Mw of 80.73×104 g/mol and 8.05×104 g/mol,the yields of (4.86±0.55)% and …
Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots,
2025
Institute of Sea Food , Zhejiang Gongshang University , Hangzhou 310012 , China
Research And Preliminary Application Of A Rapid Immunoassay Method For Eugenol Based On Quantum Dots, Junjie Sun, Xiaoxia Liu, Ziyi Chen, Yinlai Li, Lu Zhai, Qinzhou Chen, Renyao Jin
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish a rapid immunoassay method for the quantitative detection of eugenol.[Method ] The eugenol monoclonal antibody was conjugated to the surface of quantum dots by the 1-ethyl- 3- (3-dimethylaminopropyl ) carbodiimide (EDC ) and N-hydroxy-succinamide (NHS ) (EDC-NHS ) coupling method,with quantum dots as fluorescent markers.Bovine serum albumin (BSA ) was used for blocking,and fluorescence microscopy,agarose gel electrophoresis,and a microplate reader were used for identification.Furthermore,the established method was compared with high performance liquid chromatography (HPLC ).[Result ] The optimal synthesis conditions were as follows:quantum dot activation time of 30 min,conjugation pH with 8.0,antibody addition …
Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch,
2025
College of Food Science and Engineering , Xinyang Agriculture and Forestry University , Xinyang 464000 , China
Effects Of Tea Polysaccharides On The Properties Of Chestnut Starch, Lei Lei, Wenjun Fu, Lingling Yang, Haibo Liu, Daxi Sang, Qing Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the properties of chestnut starch.[Method ] Tea polysaccharides were added to chestnut starch in different proportions,and the effects of tea polysaccharides on the water-holding capacity,digestive properties,textural properties,and other properties of chestnut starch were investigated.[Result ] The water-holding and oil-holding properties of chestnut starch were the largest when tea polysaccharides were added at 1% and then gradually decreased with the increase in the proportion of tea polysaccharides added.The transparency of chestnut starch gradually increased when tea polysaccharides were added from 1% to 8% and decreased significantly when tea polysaccharides were added at 16%.Adding tea …
Research Progress In Thermo-Reversible Polysaccharide Gels,
2025
State Key Laboratory of Food Science and Resources , Jiangnan University , Wuxi 214122 , China
Research Progress In Thermo-Reversible Polysaccharide Gels, Dongxin Zhou, Ziao Zhang, Su Chen, Weimin Yang, Jiajun Sun, Shengzu Han, Anning Xie, Xiaoxiao Li, Yuxiang Bai
Journal of Food Science and Biotechnology
Thermo-reversible gels are widely applied in the processing of baking,meat,and snack products including cakes,candies,jelly,aspic,and steamed custard buns.Currently,the most effective thermo-reversible gel is gelatin,which can combine with water to form a transparent gel that melts when it is close to the temperature of the human oral cavity.However,gelatin is expensive.Therefore,researchers have turned their attention to polysaccharide gels,hoping to obtain more thermo-reversible food raw materials with different properties.The author reviews thermo-reversible polysaccharide gels from natural sources such as plants,algae,and microorganisms and those modified by physical,chemical,and enzymatic methods and summarizes the sources,structures,gelatinization mechanisms,and product applications of polysaccharide gels.It is expected to provide a reference …
Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases,
2025
Faculty of Life Science and Technology , Kunming University of Science and Technology , Kunming 650500 , China
Mechanisms Of Antioxidant Activity Of Lactic Acid Bacteria And Its Application In Oxidative Stress-Related Diseases, Yixiao Shao, Chuang Huang, Chenjian Liu, Xiaoran Li
Journal of Food Science and Biotechnology
The antioxidant properties of lactic acid bacteria are related to various probiotic activities,which can prevent and alleviate a variety of chronic diseases caused by oxidation in the host.The antioxidant mechanisms of lactic acid bacteria,including the action mechanisms of antioxidant enzymes and non-enzymatic antioxidants and regulation of host signaling pathways,were expounded.Furthermore,the application status of antioxidant properties of lactic acid bacteria in diseases of the nervous system,digestive system,and skin system were summarized.The aim is to explore how to enhance the antioxidant activity of lactic acid bacteria in oxidative stress-related diseases and give new insights into the development of related treatment strategies.
Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Reconstruction And Analysis Of Genome-Scale Metabolic Model Of Aspergillus Oryzae Fmme-S- 38, Cong Gao, Weijia Yuan, Chao Ye, Jia Liu, Liming Liu
Journal of Food Science and Biotechnology
[Objective ] A genome-scale metabolic model (GSMM ) of the high L-malic acid-producing strain Aspergillus oryzae (A.oryzae ) FMME-S- 38 was constructed and analyzed.[Method ] The new GSMM iJW1333 was established for A.oryzae FMME-S- 38 through a two-step process of draft reconstruction and model refinement.[Result ] The model integrated 1 723 biochemical reactions,1 723 metabolites,and 1 333 functional genes,achieving the prediction accuracy of 94.6% for substrate metabolism and 92.0% for cell growth.Comparative genomic analysis revealed that mutations in the genes involved in the hexose monophosphate pathway (HMP ) and oxidative phosphorylation underpinned the enhanced L-malic acid production of A.oryzae FMME-S- …
Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China;Shanghai Engineering Research Center of Food Microbiology , University of Shanghai for Science and Technology , Shanghai 200093 , China
Effects Of Extraction Methods On The Composition And Immunological Activities Of Yeast Mannoproteins, Haibo Zhang, Yongjun Xia, Guangqiang Wang, Ning Peng, Zhixian Chen, Lianzhong Ai
Journal of Food Science and Biotechnology
[Objective ] The extraction efficiency of different methods and the effects of these methods on the composition of mannoproteins extracted from yeast cell walls were compared.Furthermore,the anti-inflammatory activities of the extracted mannoprotein were evaluated.[Method ] Three yeast mannoproteins (MP-W,MP-B,and MP-E ) were extracted via high-temperature water,alkali,and enzyme-assisted extraction methods and purified via ethanol precipitation.The identification of monosaccharide composition and amino acid composition,distribution of relative molecular mass,and Fourier transform infrared spectroscopy were employed to analyze the composition and chemical structures of yeast mannoproteins.A cell experiment was carried out to examine the anti-inflammatory activity.[Result ] The yields of yeast mannoproteins extracted by …
In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging,
2025
Yunnan Botanee Bio-technology Group Co ., Ltd ., Kunming 650106 , China;Shanghai Botanee Bio-technology Group Co ., Ltd ., Shanghai 201702 , China
In Vitro Antioxidant And Anti-Inflammatory Activities Of Tea Flower Extract And Its Inhibitory Effect On Cell Aging, Shishuai Yu, Yining Hao, Bo Peng, Youyun Liu, Liping Qu
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the in vitro antioxidant and anti-inflammatory activities of Yunnan tea flower extract (TFE ) and its Inhibitory effect on cell aging.[Method ] The main active components in TFE were determined by ultraviolet (UV)-visible spectrophotometry and high performance liquid chromatography (HPLC ).The antioxidant and anti-inflammatory activities of TFE were determined by the free radical scavenging assay and UV-induced photoaging model of human keratinocytes (HaCaT ),respectively.After the aging model of fibroblasts was established,the aging level of cells was assessed by the β-galactosidase (β-gal) staining assay,and the expression of matrix metalloproteinase- 1 (MMP- 1),matrix metalloproteinase- 3 …
Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Synthesis Of N-Acetyl- Trans -4-Hydroxyproline Catalyzed By Acyltransferase, Chuanxiang Wei, Keru Chen, Yun Hu, Chao Sun, Rukang Xue, Fubao Sun, Jingfang Cheng
Journal of Food Science and Biotechnology
[Objective ] This study aims to establish the biosynthesis process of N-acetyl- trans -4-hydroxyproline (N-ATHOP ).[Method ] Escherichia coli (E.coli ) BL21 (DE3) was selected as the host to heterologously express the acyltransferase (MsAcT ) derived from Mycobacterium smegmatis.A semi-rational design was employed to construct the mutant MsAcT (S11C) capable of catalyzing the synthesis of N-ATHOP.[Result ] The catalytic reaction conditions of the mutant MsAcT (S11C) were optimized as pH 6.9,reaction temperature 49 ℃,initial trans -4-hydroxyproline (THOP ) concentration of 315.5 mmol/L,and vinyl acetate volume fraction of 10%.Under the optimized conditions,the yield of N-ATHOP synthesized under the catalysis of MsAcT …
Zeaxanthin Encapsulated With High Internal Phase Emulsions And Its Stability,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China;Key Laboratory of Industrial Biotechnology , Ministry of Education , Jiangnan University , Wuxi 214122 , China
Zeaxanthin Encapsulated With High Internal Phase Emulsions And Its Stability, Ya Li, Ying Ou, Jie Lu, Shuo Meng, Shoushuai Feng, Hailin Yang
Journal of Food Science and Biotechnology
[Objective ] This study prepared highly stable high internal phase emulsions (HIPEs ) to encapsulate zeaxanthin,thereby improving the stability and expanding the food and pharmaceutical applications of zeaxanthin.[Method ] HIPEs were prepared with the gelatin-chitosan suspension for encapsulation of zeaxanthin extracted from goji berries.Ultra-performance convergence chromatography (UPCC ) was employed to separate zeaxanthin.Confocal laser scanning microscopy (CLSM ) was employed to observe the emulsion structure.The rheological analysis was performed to characterize the emulsion,which was combined with the hydrogen peroxide and storage stability tests to evaluate the performance of encapsulation with HIPEs in improving the stability of zeaxanthin.[Result ] The separation …
Health Risk Assessment Of Multiple Mycotoxins In Rice , Maize , Soybean , And Wheat Flour Marketed In Shanghai,
2025
The Institute for Agro-food Standards and Testing Technology , Shanghai Academy of Agricultural Sciences , Shanghai 201403 , China
Health Risk Assessment Of Multiple Mycotoxins In Rice , Maize , Soybean , And Wheat Flour Marketed In Shanghai, Zuoyin Zhu, Jie Wang, Junmei Ma, Hanke Zhao, Haisheng Cheng, Chengbao Huang, Junhua Yang
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the potential health risks to consumers from exposure to multiple mycotoxins in grains.[Method ] Point and probabilistic assessment models were adopted to analyze the dietary exposure risks of adult residents in Shanghai to aflatoxins (AFs),ochratoxin A (OTA ),zearalenone (ZEN ),deoxynivalenols (DONs ),and fumonisins (FBs) through consumption of rice,maize,soybeans,and wheat flour.[Result ] The margin of exposure values (MoE ) for AFs in maize and aflatoxin G 1 (AFG 1) in soybeans exceeded 10 000,indicating no dietary exposure risk.In contrast,AFs in rice and wheat flour,as well as aflatoxin B 1 (AFB 1),aflatoxin B 2 (AFB …
Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles,
2025
Yueyang Inspection and Testing Center ,Yueyang 410042 ,China;Hunan Provincial Pickles Product Quality Supervision and Inspection Center , Yueyang 410042 , China
Identification And Spoilage Capacity Analysis Of Main Spoilage Bacteria In Pickles, Li Zhu, Huimin Li, Liejiang Yuan, Cancan Peng, Bo Zhou, Sheng Song, Haizhi Wu, Mingyang Zhu, Fangmei Wang, Yu Liu, Yuqian Hu, Ge Li, Yiqing Sun, Yi Zhou
Journal of Food Science and Biotechnology
[Objective ] This study aims to identify the main spoilage bacteria and evaluate their spoilage capacity in the spoilage process of pickles.[Method ] High-throughput sequencing was employed to analyze the microbial community dynamics in the samples of pickles at the early,middle,and late stages of spoilage.The key spoilage bacteria were isolated,purified,and then identified through a combination of colony morphology observation,biochemical analysis,and 16S rDNA/ITS sequencing.Re-inoculation experiments were carried out for further validation of the primary spoilage bacteria in pickles.[Result ] Acinetobacter and Bacillus were the dominant spoilage bacterial genera across the three spoilage stages.Notably,Bacillus exhibited rapid growth over time,with the relative abundance …
