Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation,
2025
College of Food Science and Engineering , Ningbo University , Ningbo 315211 , China
Research Progress In The Application Of Tea Polyphenols In Meat Processing And Preservation, Yanting Gao, Huanhuan Li, Teng Xu, Ke Zhao, Mingwei Su, Liwen Wu, Honggang Tang
Journal of Food Science and Biotechnology
Tea polyphenols are the general term for polyphenols in tea,mainly including catechins,flavonoids,and anthocyanins,which have antioxidant and antimicrobial properties.The author reviewed the structural characteristics and the antioxidant and antibacterial mechanisms of tea polyphenols.Additionally,the application of tea polyphenols in the processing and preservation of meat products is summarized,providing a theoretical basis for their use in meat products.Studies have shown that tea polyphenols can achieve anti-oxidation by scavenging free radicals,chelating metal ions,and inhibiting oxidase activity.Moreover,tea polyphenols can inhibit cell growth and proliferation by inhibiting the activities of enzymes involved in cell metabolism and damaging the cell membrane system.As natural antioxidants,tea polyphenols can significantly …
Safety Properties , Growth Characteristics , And Fermentation Performance Of Tetragenococcus Halophilus,
2025
College of Food Science , South China Agricultural University , Guangzhou 510642 , China
Safety Properties , Growth Characteristics , And Fermentation Performance Of Tetragenococcus Halophilus, Ranhui Lyu, Yanhua Zhong, Yang You, Runhan Yuan, Enyi Guan, Shan Duan
Journal of Food Science and Biotechnology
[Objective ] The physiological characteristics vary greatly among different strains of Tetragenococcus halophilus (T.halophilus ).By comparing some indicators of safety properties,growth characteristics,and fermentation performance of T.halophilus strains from different sources,the authors aim to establish a regular understanding of this bacterial species.[Method ] Seventeen strains of T.halophilus derived from different aquatic products and soy sauce mash were selected,and their antibiotic resistance,biogenic amine production,hemolytic activity,growth under different environmental conditions,and flavor characteristics after fermenting soybean hydrolysate were determined.Additionally,correlation analysis was conducted to identify the associations among various indicators.[Result ] In terms of safety properties,the 17 strains were generally sensitive to penicillin,vancomycin,and tetracycline,but resistant …
Analysis Of Volatile Aroma Components Of Black Tea Samples From Different Tea Varieties In Guangxi Based On Hs-Spme-Gc-Ms Technology,
2025
Guangxi South Subtropical Agricultural Sciences Research Institute , Chongzuo 532415 , China
Analysis Of Volatile Aroma Components Of Black Tea Samples From Different Tea Varieties In Guangxi Based On Hs-Spme-Gc-Ms Technology, Yunxiong Zhao, Hongyu Feng, Ziping Li, Baogui Liu, Yunxian Wang, Jingjing Yang, Lianfeng Luo
Journal of Food Science and Biotechnology
[Objective ] This study aims to analyze the characteristics of volatile aroma components and key aroma components of black tea samples from different tea varieties in Guangxi.[Method ] The aroma components of 12 black tea samples from Guangxi were identified by sensory quality evaluation,headspace solid-phase microextraction-gas chromatography-mass spectrometry （HS-SPME-GC-MS ）,hierarchical cluster analysis,and orthogonal partial least squares-discrimination analysis (OPLS-DA ).[Result ] The black tea samples from different tea varieties showed different aroma characteristics and mainly presented a floral fruity aroma or a sweet aroma.The black tea from ‘Wuniuzao ’ demonstrated the highest comprehensive score (91.20),being a representative of black tea with …
Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes,
2025
College of Food Science and Technology , Wuhan Business University , Wuhan 430056 , China
Construction , Structural Characterization , And Antioxidant Activity Evaluation Of Yam Polysaccharide-Selenium Nanoparticle Complexes, Yidong Xiao, Lanmei Ou, Mengmeng Zhao
Journal of Food Science and Biotechnology
[Objective ] This study explored the structural characteristics and in vitro antioxidant activity of yam polysaccharide-selenium nanoparticle (YP-SeNPs ) complexes.[Method ] Yam polysaccharide (YP) were obtained through hot water extraction and then purified by purification,diethylaminoethyl cellulose 52 (DEAE-52) column chromatography,and dialysis.With YP as a template,YP-SeNPs were prepared with different Se/YP mass ratios (1∶3,1∶1,and 4∶3) in an oxidation-reduction system comprising ascorbic acid (VC) and sodium selenite (Na 2SeO 3）.The complexes were characterized via multiple techniques,including visual observation of solution color,ultraviolet-visible absorption (UV-vis ) spectroscopy,particle size measurement,transmission electron microscopy (TEM ),scanning electron microscopy (SEM ),atomic force microscopy (AFM ),Fourier-transform infrared spectroscopy (FT-IR …
Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria,
2025
Physical Education College , Suihua University , Suihua 152001 , China
Preparation And Anti-Exercise Fatigue Effect Evaluation Of Γ-Aminobutyric Acid-Enriched Milk Fermented With Lactic Acid Bacteria, Dong Liu, Yuning Yang, Fangfei Dou, Yuxing Li, Minghai Ju
Journal of Food Science and Biotechnology
[Objective ] This study aims to prepare γ-aminobutyric acid (GABA )-enriched milk fermented with lactic acid bacteria and evaluate its anti-exercise fatigue effect.[Method ] High GABA-producing lactic acid bacteria were selected and used to ferment milk with a commercial starter culture.A mouse model of exercise fatigue was established by four weeks of exhaustive swimming training.After four weeks,the exhaustive swimming time,serum biochemical indicators,antioxidant indicators,and energy storage substance levels were measured and analyzed.[Result ] Lactobacillus plantarum (L.plantarum ) LP- 5 was selected as the strain for fermentation,and its GABA yield reached (1.340±0.003) g/L.The fermented milk showed the titratable acidity of (46.29±2.01) ºT,the …
Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes,
2025
State Key Laboratory of Space Medicine , China Astronaut Research and Training Center , Beijing 100094 , China
Research Progress In Application Of Ultra-High Pressure Sterilization In The Processing Of Prepared Dishes, Haisheng Dong, Peng Zang
Journal of Food Science and Biotechnology
As a non-thermal sterilization technology,ultra-high pressure sterilization can protect the thermosensitive nutrients and maintain the texture of food,demonstrating unique technical advantages in the processing of prepared dishes.By a review of domestic and foreign literature and performance of relevant equipment,this paper introduced the principle,equipment,and parameters of ultra-high pressure sterilization.Considering the parameters of ultra-high pressure sterilization and the effects of food ingredients on the pressure tolerance of different microbial species,the factors affecting the effect of ultra-high pressure sterilization were analyzed.In addition,the effects of ultra-high pressure sterilization on the physical and chemical properties,sensory quality,enzyme activity,microorganisms count and bioactive substances of prepared dishes were …
Research Progress In Biological Activities , Mechanisms Of Action , And Applications Of Antimicrobial Peptides,
2025
School of Food and Bioengineering , Changsha University of Science and Technology , Changsha 410114 , China
Research Progress In Biological Activities , Mechanisms Of Action , And Applications Of Antimicrobial Peptides, Yuxin Jiao, Ruolan Shan, Xufeng Wang, Maolong Chen, Li Wen, Yunhui Cheng, Hao Wu
Journal of Food Science and Biotechnology
The widespread abuse of traditional antibiotics has led to the emergence and development of antimicrobial peptides due to the resultant bacterial resistance.Antimicrobial peptides demonstrate high efficacy in combating infectious pathogens and possess unique antimicrobial mechanisms and biocompatibility,effectively reducing the bacterial resistance rate.The high structural adaptability endows antimicrobial peptides with multiple biological activities,enabling targeted action against diverse types of pathogens and abnormal cells.Accordingly,antimicrobial peptides exhibit a promising development prospect.Enhanced stability contributes to prolonging the in vivo half-life and improving the bioavailability of antimicrobial peptides,which holds crucial importance for improving the clinical translation value of antimicrobial peptides.The author summarizes the research progress …
Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles,
2025
School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Mechanisms Of The Quality Changes Of Polygonati Rhizoma Processed By Steaming-Drying Cycles, Zicong Yu, Liuping Fan
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of steaming-drying cycles on the quality changes of Polygonati rhizoma and optimize the processing technology to enhance its application value.[Method ] Slices of Polygonati rhizoma processed by 0~5 steaming-drying cycles were prepared.The drying rate and drying kinetics during the drying stage after each cycle were analyzed,and a drying model was fitted.Changes in color difference,appearance,hardness,and mass fractions of polysaccharides,reducing sugars,polyphenols,flavonoids,saponins,and calcium oxalate raphides were measured.[Result ] The number of steaming-drying cycles significantly affected the quality of Polygonati rhizoma.The drying rate decreased as the drying time increased,conforming to the Modified Page model,while the …
Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey,
2025
Institute of Apicultural Research , Chinese Academy of Agricultural Sciences , Beijing 100093 , China;State Key Laboratory of Resource Insects , Beijing 100093 , China;Key Laboratory of Bee Products for Quality and Safety Risk Assessment , Ministry of Agriculture and Rural Affairs , Beijing 100093 , China
Determination Of Microorganisms And Analysis Of Factors Influencing Osmophilic Yeasts In Apis Cerana Honey, Mei Song, Hongxia Li, Zhaolong Liu, Dong Qiao, Na Li, Lanzhen Chen
Journal of Food Science and Biotechnology
[Objective ] This study determined the mold count,total plate count,and osmophilic yeast count in Apis cerana honey and explored the correlations of osmophilic yeasts with quality parameters and storage conditions of the honey.[Method ] A total of 105 Apis cerana honey samples were used for the determination of mold count,total plate count,and osmophilic yeast count,as well as odor,moisture,phenolic acids,and carbohydrates.The correlations between quality indexes and osmophilic yeasts and the effects of different storage conditions on osmophilic yeast count were explored.[Result ] Among the 105 samples,1,1,and 67 samples showed the mold count,total plate count,and osmophilic yeast count exceeding the national standards.All …
Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor,
2025
School of Life Sciences and Health Engineering , Jiangnan University , Wuxi 214122 , China;School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Photosensitizer-Loaded Glycogen For Photodynamic Therapy Of Tumor, Keke Ma, Jiurui Fang, Huijie Zhang, Jinghua Chen
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to synthesize aminated glycogen (NG) for loading of the photosensitizer TTVBA and construct a nano drug delivery system (NGT NPs ) for photodynamic therapy (PDT ) of tumors.[Method ] Positively charged NG was synthesized through modification of glycogen with diethylenetriamine (DETA ).Then,TTVBA was encapsulated by NG via electrostatic interaction to prepare NGT NPs.The prepared NGT NPs were characterized by infrared spectroscopy,dynamic light scattering (DLS ),and transmission electron microscopy (TEM ).The hemolysis assay was performed to evaluate the biocompatibility of NGT NPs.The reactive oxygen species (ROS ) generation capability of cells treated with …
Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1,
2025
State Key Laboratory of Food Nutrition and Safety , Tianjin University of Science & Technology , Tianjin 300457 , China
Prokaryotic Expression , Purification , And Polyclonal Antibody Preparation Of Chlamydomonas Reinhardtii Channelrhodopsin 1, Jiurong Wang, Bin Xue, Zhenchuan Fan
Journal of Food Science and Biotechnology
[Objective ] This study aims to prepare a highly specific antibody against channelrhodopsin- 1 (ChR 1) from Chlamydomonas reinhardtii (C.reinhardtii ) and investigate its regulatory mechanisms in cellular phototaxis and negative phototaxis.[Method ] The recombinant expression vector pET- 28a(+)-chr1 was constructed and transformed into Escherichia coli (E.coli ) BL21 (DE3) competent cells.The expression of the fusion protein 6×His∷ChR 1 was induced with IPTG,and the obtained protein was purified by nickel-affinity chromatography.The purified protein was used to immunize New Zealand white rabbits for the production of ChR 1 antiserum.ChR 1-specific IgG antibodies were enriched from the antiserum through protein A purification.The …
Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Engineering Escherichia Coli Tolerance To Improve Succinic Acid Production, Jingyu Pan, Yongsheng Tang, Jia Liu, Cong Gao, Xiaomin Li, Wei Song, Wanqing Wei, Guipeng Hu, Jing Wu, Liming Liu, Xiulai Chen
Journal of Food Science and Biotechnology
[Objective ] This study aims to enhance the high osmotic pressure tolerance of Escherichia coli (E.coli ) for the efficient production of succinic acid.[Method ] Adaptive evolution,transcriptomic analysis,reverse metabolic engineering,and fermentation optimization were employed for improving E.coli tolerance to high osmotic pressure.[Result ] By analyzing the key factors influencing succinic acid production by fermentation,osmotic pressure was identified as the critical factor reducing succinic acid production efficiency.A mutant strain,E.coli FN-6,with tolerance to high osmotic pressure was obtained through adaptive evolution.The key gene cusC responsible for osmotic pressure tolerance was identified by transcriptomic analysis.After optimization of the expression level of cusC,the engineered …
Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil,
2025
School of Food Science and Technology , Dalian Polytechnic University , Dalian 116034 , China
Study On The Antioxidant Effects Of Natural Antioxidants And Their Derivatives On Anchovy (Engraulis Japonicus ) Oil, Xuyang Ding, Fujun Liu, Fawen Yin, Dayong Zhou
Journal of Food Science and Biotechnology
[Objective ] The aim of this study is to evaluate and improve the antioxidant effects of natural antioxidants and their derivatives on anchovy (Engraulis japonicus ) oil.[Method ] The 60 ℃ accelerated oxidation model was used to evaluate the antioxidant effects of phytic acid,tea polyphenols (TP),rosemary extract,ascorbyl palmitate,vitamin E,tea polyphenol palmitate (TPP),and bamboo leaf extract on anchovy oil based on the peroxide value (POV ),thiobarbituric acid reactive substances (TBARS ) value,relative intensity of free radicals,and fatty acid composition.[Result ] TPP (single antioxidant ) and TPP+TP (composite antioxidant ) demonstrated the strongest antioxidant effects on anchovy oil.[Conclusion ] Although the antioxidant …
Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7,
2025
School of Food Science and Engineering , Shaanxi University of Science and Technology , Xi’an 710021 , China
Establishment Of Surface-Enhanced Raman Spectroscopy For Detection Of Escherichia Coli O157:H7, Panxue Wang, Cen Li, Xiang Li, Ying Liu, Guoliang Li
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the detection specificity and sensitivity for Escherichia coli (E.coli ) O157:H7.[Method ] A high ly specific and sensitive method for the detection of E.coli O157:H7 was established based on magnetic separation and signal amplification strategies combined with surface-enhanced Raman spectroscopy (SERS ).Aptamers were used as recognition elements and combined with magnetic beads and polystyrene (PS) microspheres to construct a separation probe (MB@Apt ) and a signal amplification Raman tag (4-MBN@PS@Apt ),respectively.[Result ] In the presence of E.coli O157:H7,MB@Apt and 4-MBN@PS@Apt formed a “sandwich ” structured composite (MB@Apt/ E.coli O157:H7/4-MBN@PS@Apt ),which could be rapidly …
Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay,
2025
Wuxi Food Safety Inspection and Test Center , Wuxi 214142 , China ;Technology Innovation Center of Special Food for State Market Regulation , Wuxi 214142 , China ;School of Food Science and Technology , Jiangnan University , Wuxi 214122 , China
Rapid Determination Of Vitamin B 1 In Infant Formula Milk Powder By Fluorescent Microsphere Immunochromatographic Assay, Qianqian Lu, Ting Yang, Yongwei Feng, Jialin Hu, Hua Kuang, Chuanlai Xu, Lingling Guo
Journal of Food Science and Biotechnology
[Objective ] A fluorescent immunochromatographic assay was established based on vitamin B 1 monoclonal antibody to measure the vitamin B 1 in infant formula milk powder.[Method ] The performance of vitamin B 1 monoclonal antibody was evaluated.On this basis,a highly sensitive fluorescent immunochromatographic test strip was developed.[Result ] The vitamin B 1 monoclonal antibody showed high sensitivity,affinity,and specificity.The fluorescent immunochromatographic test strip showed a visual limit of detection (vLOD ) of 10 μg/kg,a cut-off value of 200 μg/kg,and a calculated limit of detection (cLOD ) of 2.056 μg/kg.In addition,the recovery rate of this method was 97.3%~115.4%.The test results of the …
Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods,
2025
College of Food and Bioengineering , Henan University of Science and Technology , Luoyang 471000 , China;Henan International Joint Laboratory of Food Green Processing and Quality Safety Control , Luoyang 471000 , China
Colloidal Carbon-Labelled Immunochromatographic Assay For Rapid Detection Of Marbofloxacin Residues In Animal-Derived Foods, Xiujin Chen, Biao An, Jian Tao, Xinhao Yin, Yao Wang, Huawei Niu, Zhaozhou Li, Min Zhang
Journal of Food Science and Biotechnology
[Objective ] A colloidal carbon-labelled immunochromatographic assay with competitive inhibition was developed for the detection of marbofloxacin (MBF ) residues in milk powder and chicken.[Method ] The anti-MBF polyclonal antibody prepared in the author 's laboratory was employed to produce the colloidal carbon-labelled immunoprobe.The effects of pH of reaction system,resuspension solution,treatment solution of sample pad,standard dilution buffer,mass concentration of colloidal carbon,mass concentration of primary antibody,mass concentration of coating antigen,and mass concentration of secondary antibody on the MBF colloidal carbon-labelled immunochromatographic assay were investigated.The sensitivity,specificity,stability,and accuracy of the method were determined.[Result ] Under optimal conditions,the cut-off value of the method was …
Construction Of Staphylococcus Aureus Detection Method Based On,
2025
School of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , China
Construction Of Staphylococcus Aureus Detection Method Based On, Silver Nanoclusters Dna, Xiaotong Lu, Jinzhao Li, Xinjun Du
Journal of Food Science and Biotechnology
[Objective ] A simple and sensitive detection method for Staphylococcus aureus is established for the efficient detection of this pathogen in food.[Method ] Targeting Staphylococcus aureus,a simple and sensitive fluorescent detection method for Staphylococcus aureus,EXPAR-P-AgNCs,was developed based on exponential amplification reaction (EXPAR ) combined with DNA silver nanoclusters (P-AgNCs ).A recognition probe was designed to initiate EXPAR in the presence of Staphylococcus aureus,generating a large amount of target DNA(T).The binding of T to hairpin P displaced the G-rich strand from P-AgNCs,resulting in a decrease in the relative fluorescence intensity of P-AgNCs and enabling the detection of Staphylococc us aureus.[Result ] …
Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu,
2025
Guizhou Xijiu Co ., Ltd., Zunyi 564622 , China
Physicochemical Indicators , Microbial Diversity , And Volatile Substances In The Qupi And Quxin Of High-Temperature Daqu, Dan Xie, Cheng Wu, Wenyu Zhao, Jianfeng Hu, Pingyan Cheng, Yuanlin Bi, Junlin Yang, Xiaolong You, Diqiang Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the physicochemical indicators,microbial diversity,and volatile substances in the Qupi (surface ) and Quxin (core) of high-temperature Daqu.[Method ] High-throughput sequencing and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS ) were employed to compare the microbial diversity,volatile substances,and physicochemical indicators between Qupi and Quxin of three types of Daqu.[Result ] The moisture mass fraction in Qupi of the three types of Daqu was lower than that in Quxin (P<0.05),and the moisture mass fraction was the lowest in the Qupi of white Daqu (WP) and the highest in the Quxin of yellow Daqu (YX).The acidity of Qupi and Quxin were in the order of Quxin of black Daqu (BX) > YX > Qupi of black Daqu (BP) > Quxin of white Daqu (WX) > Qupi of yellow Daqu (YP) > WP.High-throughput sequencing results showed that Firmicutes and …
Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China
Heterologous Expression And Application Of A Thermophilic Lytic Polysaccharide Monooxygenase, Le Xie, Longyun Peng, Yun Hu, Hongyan Ren, Haiyan Sun, Fubao Sun
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the application of a novel thermophilic lytic polysaccharide monooxygenase (LPMO ) in the efficient cellulase-mediated hydrolysis of lignocellulosic substrates.[Method ] The thermophilic enzyme mgLPMO 10,derived from a compost metagenome,was heterologously expressed in Escherichia coli (E.coli ).Its expression level was improved by modifying the signal peptide of the expression vector and induction conditions (temperature,IPTG concentration,and induction time ).The enzyme was then characterized in terms of the optimal temperature,optimal pH,thermal stability,pH stability,and the effects of metal ions on its enzyme activity.Subsequently,the synergistic hydrolysis efficiency of mgLPMO 10 with two thermophilic endoglucanases (TnCelB and DtCelB ) …
Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A,
2025
National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing , Jiangnan University , Wuxi 214122 , China
Preparation , Performance , And Application Of A High-Performance Affinity Chromatography Medium Based On Staphylococcus Aureus Protein A, Zihao Guo, Chaoqing Yan, Haifeng Xia
Journal of Food Science and Biotechnology
[Objective ] Staphylococcus aureus protein A (SPA ) is widely used for the purification of monoclonal antibodies due to its high specificity for immunoglobulin G (IgG).However,the high costs of commercial media have limited the further development of SPA.To address this issue,this paper aims to prepare a high-performance affinity chromatography medium based on SPA as the substitute of introduced affinity chromatography media.[Method ] SPA expressed by the recombinant strain was purified via Ni2+ affinity chromatography and then coupled to the Rigose HF matrix to prepare an self-prepared affinity chromatography medium.[Result ] The self-prepared medium had a static saturated adsorption capacity of …
