Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia,
2025
Food Laboratory of Zhongyuan , Luohe 462300 , China
Therapeutic Mechanisms Of A Lilium Brownii -Plantago Asiatica L.Compound Solid Beverage Against Hyperuricemia, Feng Li, Jiawei Huang, Xiaowen Guo, Lulu Jiang, Hongyan Zhao, Qiang Li, Chenxiang Sun, Shuying Feng, Qianqian Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the ameliorative effects of a Lilium brownii -Plantago asiatica L.compound solid beverage on hyperuricemia and to elucidate its potential mechanisms.[Method ] Network pharmacology was employed to screen the active constituents,potential targets,and related pathways.Key targets were identified based on a protein-protein interaction (PPI) network.Molecular docking was conducted between the key proteins and active constituents.An animal experiment was performed to validate the effects of the compound solid beverage on serum uric acid levels,renal function indices,activities of uric acid-producing enzymes,and the levels of renal urate transporters.[Result ] Network pharmacology analysis indicated that Lilium brownii and Plantago …
Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp.,
2025
Jiangsu Zhongnongke Food Engineering Co ., Ltd ., Suqian 223800 , China
Research Progress In Enrichment Of Trace Elements Zinc And Selenium In Pleurotus Spp., Jing Zhang, Mengmeng Xu, Liting Zhao, Cui Li, Lei Chen, Song Ming, Zhongyang Ding
Journal of Food Science and Biotechnology
Zinc and selenium are essential trace elements for maintaining human normal physiological function.Deficiency of zinc or selenium can result in immune dysfunction,chronic diseases such as cardiovascular diseases and hyperlipidemia,and even increase the risk of cancer.The human body cannot independently synthesize these trace elements,which can only be obtained from the daily diet.However,natural food sources have low content of zinc and selenium,which is insufficient to meet the bod y’s needs.Therefore,developing fortified foods with trace nutrients is an important approach to meeting residents ’ requirements.Edible fungi serve as natural carriers for enriching trace elements and ideal dietary supplements.Pleurotus spp.,as a typical example,not only …
Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli,
2025
School of Biotechnology , Jiangnan University , Wuxi 214122 , China ;Key Laboratory of Industrial Biotechnology of Ministry of Education , Jiangnan University , Wuxi 214122 , China
Construction And Optimization Of An Efficient Tyrosol Biosynthesis System In Escherichia Coli, Yizhe Xiong, Shougang Wang, Zhiming Rao, Meijuan Xu
Journal of Food Science and Biotechnology
[Objective ] An efficient biosynthetic system was constructed in Escherichia coli to achieve high-yield production of tyramine and its key derivative tyrosol.[Method ] Through the analysis of protein expression,enzymatic properties,and whole-cell catalysis results,tyrosine decarboxylase (TDC ) derived from Enterococcus faecalis was selected as the key enzyme for tyramine synthesis.Subsequently,tyramine oxidase (TYO ) was introduced to construct the TDC-TYO pathway for tyrosol production.Meanwhile,the LAAD-PDC pathway for tyrosol synthesis was established.A dual-enzyme co-expression system was established,and the effects of tandem enzyme order,production pathways,and key enzyme sources on tyrosol yield were analyzed.The recombinant strain B 1,carrying the plasmid pET 28a-EfTDC-TYO,was obtained through whole-cell …
Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy,
2025
Key Laboratory of Industrial Biotechnology of the Ministry of Education , Jiangnan University , Wuxi 214122 , China;Yixing Institute of Food and Biotechnology Co ., Ltd., Wuxi 214200 , China
Promoting The Synthesis Of Salicylic Acid In Escherichia Coli Based On Metabolic Engineering And Multi-Enzyme Cascade Strategy, Kai Wang, Xuewei Pan, Taowei Yang, Zhiming Rao
Journal of Food Science and Biotechnology
[Objective ] The production of the aromatic compound salicylic acid (SA) is limited by its inhibitory effects on enzymes related to cellular energy metabolism.This study aims to develop a strategy for avoiding the impact of SA on enzymes related to cellular energy metabolism and increase the yield of SA.[Method ] A highly efficient Escherichia coli strain was designed to improve SA production by optimizing metabolic pathways and enzyme systems.[Result ] The genome-scale metabolic model GEMS 1515 was employed to predict the key bottle necks in the phosphoenolpyruvic acid (PEP) pathway and SA biosynthesis pathways.The SA yield was successfully increased from …
Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum,
2025
College of Biotechnology and Pharmaceutical Engineering , Nanjing Tech University , Nanjing 211816 , China ;Nanjing Hi-Tech Biological Technology Research Institute Co ., Ltd., Nanjing 210032 , China
Fermentation Technology Of L-Lysine Production By Immobilized Corynebacterium Glutamicum, Qingguo Liu, Hanjie Ying, Wenjun Sun
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the feasibility of the fermentation process for L-lysine production by immobilized Corynebacterium glutamicum.[Method ] Firstly,the effects of different volume fractions of seed medium on the fermentation stability of immobilized Corynebacterium glutamicum were examined.Subsequently,the impacts of immobilization carriers with different specifications on the fermentation performance were analyzed.Finally,a comparative analysis was conducted on the differences between the fermentation processes with free and immobilized Corynebacterium glutamicum.[Result ] The addition of 5% volume fraction of seed medium adequately sustained the growth and metabolic activities of Corynebacterium glutamicum.Higher volume fractions diverted carbon sources towards bacterial growth,thereby limiting product …
Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast,
2025
College of Chemistry and Bioengineering , Guilin University of Technology , Guilin 541004 , China
Reduction Of Methanol Content By Protoplast Fusion In Fruit Wine Yeast, Huixiang Zhang, Nan Lin, Jiaru Wu, Xin Cheng, Weiwu Liang, Shijun Yang
Journal of Food Science and Biotechnology
[Objective ] This study aims to breed a fruit wine yeast strain with low methanol production by protoplast fusion.[Method ] With yeast strains LAB and XR as parents,protoplast fusion was employed to breed the yeast strains for fruit wine fermentation with a low mass concentration methanol and a high volume fraction ethanol.The process of yeast protoplast preparation was optimized by single factor and orthogonal experiments with the formation rate,regeneration rate,and preparation rate of protoplasts as indicators.The parental protoplasts were inactivated by ultraviolet radiation and heating,and 35 g/dL PEG- 6000 was used for chemical fusion after inactivation.[Result ] The optimum conditions …
Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes,
2025
College of Enology , Northwest A&F University , Yangling 712100 , China
Effects Of Metschnikowia Pulcherrima And 24-Epibrassinolide On Postharvest Preservation Of Grapes, Jiayan Qu, Huiping Niu, Bowen Li, Xiao Wang, Lujun Wang, Zhumei Xi, Xuefei Wang
Journal of Food Science and Biotechnology
[Objective ] This study aims to investigate the effects of different treatments on the postharvest preservation of grapes.[Method ] The impacts of 0.4 mg/L 24-epibrassinolide (EBR ),1×109 CFU/mL Metschnikowia pulcherrima,and their combination (YBR ) on postharvest storage of ‘Shine Muscat ’ grapes were evaluated.[Result ] Compared with the control (CK) group,EBR,Metschnikowia pulcherrima,and YBR treatments effectively reduced the water loss rate,maintained the firmness,and delayed the declines in protopectin and stem chlorophyll content of grape.In the later storage stage,YBR treatment delayed the decrease in flavonoid mass fraction,which was 12.95% higher than that of CK group.At the same time,YBR treatment reduced the mass …
Application Of A Rapid Test Strip Method In Quantitative Detection Of Microorganisms In Chilled Meat,
2025
College of Life Sciences , Linyi University , Linyi 276005 , China;Department of Biotechnology , The University of Suwon , Suwon 18323 , South Korea
Application Of A Rapid Test Strip Method In Quantitative Detection Of Microorganisms In Chilled Meat, Yanzhen Zhang, Ning Liu, Wei Wang, Xianqi Yao, Laixue Ni, Yunguo Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to evaluate the feasibility of using the rapid test strip method for detecting key microbial quality control indicators in chilled meat,specifically the total plate count and Escherichia coli /coliforms.[Method ] The performance of the rapid test strip method was assessed through repeatability tests,and the national standard plate count method was used as the reference for method comparison.Statistical significance of the quantification results was analyzed by a t-test,and linear regression was adopted to analyze the correlation.[Result ] The repeatability deviation of the rapid test strip method (r≤0.25) met the requirements of ISO 4833—1:2013.No significant difference was …
Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey,
2025
School of Liquor and Food Engineering , Guizhou University , Guiyang 550025 , China ;Guizhou Xijiu Investment Holding Group Co ., Ltd ., Zunyi 564662 , China
Effects Of Single And Mixed Fermentation On Flavor , Composition , And Antioxidant Activity Of Soybean Whey, Taotao Wei, Qian Chen, Xiangjie Tao, Bin Tang, Fusheng Ren, Ping Hu
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the impacts of single strain fermentation and mixed fermentation on the flavor,composition,and antioxidant activity of soybean whey.[Method ] Gas chromatography-ion mobility spectrometry (GC-IMS ) was coupled with E-nose and high-performance liquid chromatography (HPLC ) to a nalyze the flavor and function of soybean whey fermented by the single and mixed strains.[Result ] Compared with single-strain fermentation by Limosilactobacillus fermentum HJS- 1 or Kluyveromyces marxianus HJS- 2,mixed fermentation (Limosilactobacillus fermentum HJS- 1 + Kluyveromyces marxianus HJS- 2) effectively improved the levels of functional substances and antioxidant activity of soybean whey.Compared with non-fermented soybean whey,mixed …
Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli,
2025
State Key Laboratory of Bioreactor Engineering , East China University of Science and Technology , Shanghai 200237 , China
Efficient Catalytic Synthesis Of 5-Methylpyrazine- 2-Carboxylic Acid By Recombinant Escherichia Coli, Luxi Gan, Heng Gong
Journal of Food Science and Biotechnology
[Objective ] This study aimed to improve the yield and conversion rate of 5-methylpyrazine- 2-carboxylic acid (MPCA ) through biocatalysis.[Method ] Escherichia coli (E.coli）was used as the model organism to construct an MPCA synthesis system,and the whole-cell catalytic reaction conditions of recombinant E.coli were optimized.In addition,the parental host strain was subjected to ultraviolet mutagenesis for the screening of host strains tolerant to high mass concentrations of 2,5-dimethylpyrazine (DMP ).[Result ] The recombinant mutagenized strain could completely convert 10 g/L DMP to MPCA within 12 h in a 10 mL reaction system,achieving a yield of 12.9 g/L and a corresponding molar …
Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation,
2025
School of Food Engineering , Harbin University , Harbin 150086 , China
Goat Milk Formula Powder Ameliorates Lipopolysaccharide-Induced Intestinal Inflammation, Yi Shan, Xiaoling Liu, Shumei Wang
Journal of Food Science and Biotechnology
[Objective ] This study aimed to investigate the effects of goat milk formula powder on lipopolysaccharide (LPS)-induced intestinal inflammation and barrier impairment in mice.[Method ] Histopathological observation,real-time quantitative PCR,and enzyme-linked immunosorbent assay were used to assess changes in intestinal inflammation levels and barrier function-related indicators in mice following intervention with goat milk formula powder.Additionally,the composition of the gut microbiota was analyzed to elucidate its potential mechanisms of action.[Result ] Goat milk formula powder alleviated intestinal histopathology and increased the number of goblet cells.Compared with the model group,the goat milk formula powder intervention reduced the mass fractions of pro-inflammatory cytokines TNF- …
Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein,
2025
College of Life Sciences and Medicine , Zhejiang Sci-Tech University , Hangzhou 310018 , China;Shaoxing Academy of Biomedicine , Zhejiang Sci-Tech University , Shaoxing 312369 , China
Design , Expression , And Stability Evaluation Of A Superoxide Dismutase-Catalase Fusion Protein, Zhuo Jiang, Weina Lu, Qi Lin, Zhan Luo, Zhengbin Lyu, Zuoming Nie
Journal of Food Science and Biotechnology
[Objective ] This study aims to design a fusion protein with high activities and high stability of superoxide dismutase (SOD ) and catalase (CAT ).[Method ] Using AcSOD 11 and FeCAT proteins as the research subjects,three fusion proteins of SOD and CAT were designed:AcSOD 11-(GGGGS )*3-FeCAT,FeCAT-(GGGGS )*3-AcSOD 11,and FeCAT- (EAAAK )*3-AcSOD 11,which were named AcSOD 11-G3-FeCAT,FeCAT-G3-AcSOD 11,and FeCAT-E 3-AcSOD 11,respectively.Soluble expression of these three fusion proteins was achieved in Escherichia coli BL21(DE3).The thermal stability and pH stability of the three fusion proteins were then assessed.[Result ] The three fusion proteins showed no significant difference in the specific activity of SOD,while …
Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies,
2025
University of Nebraska-Lincoln
Defining The Causal Contribution Of The Gut Microbiota To Cardiomyopathy Pathogenesis As A Basis For Developing Microbiome-Targeted Therapies, Xu Shi
Department of Food Science and Technology: Dissertations, Theses, and Student Research
Heart failure (HF) is a leading cause of morbidity and mortality worldwide, placing substantial clinical and economic burdens on society. Although advances in therapy have improved patient outcomes, the biological mechanisms that drive HF onset and progression remain incompletely understood. Increasing evidence suggests that the gut microbiota, the trillions of microorganisms residing in the gastrointestinal tract, plays a key role in some forms of heart disease, especially cardiovascular disease. Moreover, gut microbial dysbiosis has been associated with systemic inflammation, metabolic imbalance, and immune dysregulation, all of which contribute to the pathophysiology of HF. Cardiomyopathy, a primary disorder of the heart …
Analysis Of Aroma Volatile Compounds In Black Tea Produced From The Large-Leaf Variety In Northern Guangxi Based On Hs-Spme-Gc-Ms,
2025
Tea Research Institute of Guangxi , Guilin 541004 , China;Guangxi Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization , Guilin 541004 , China;Guangxi Field Scientific Observation and Research Station for Tea Resources , Guilin 541004 , China
Analysis Of Aroma Volatile Compounds In Black Tea Produced From The Large-Leaf Variety In Northern Guangxi Based On Hs-Spme-Gc-Ms, Ting Chen, Chunyu Guo, Yingqi Wu, Min Li, Yuelan Pang, Yan Qing
Journal of Food Science and Biotechnology
[Objective ] This study explores the unique flavor characteristics and volatile compounds of black tea produced from the large-leaf variety in northern Guangxi,aiming to reveal the material basis of aroma formation of this variety.[Method ] Sensory evaluation,determination of biochemical parameters,headspace-solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS ),and odor activity value (OAV ) analysis were performed in combination with chemometrics analysis to analyze the flavor characteristics and volatile compounds of the black tea.[Result ] The black tea samples produced from the large-leaf variety in northern Guangxi had similar aroma properties and were characterized by distinct floral and fruity aromas.A total of 88 volatile …
Advances In The Discovery Of Active Natural Products By Large Fragment Cloning,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China
Advances In The Discovery Of Active Natural Products By Large Fragment Cloning, Dandan Zeng, Jiayu Chen, Tianlu Mo
Journal of Food Science and Biotechnology
Active natural products have diverse structures and activities and can be a major source of small molecule drugs.Given the emergence of multi-drug resistant bacteria and superbacteria,there is an urgent need to develop new active natural products.Large fragment cloning has been an important tool for the discovery of active natural products in recent years,and it has demonstrated obvious advantages in obtaining the biosynthetic gene clusters of active natural products.This approach,combined with efficient cluster-targeted screening methods,has significantly accelerated the discovery of active natural products.This review summarizes a wide variety of active natural products.Furthermore,this paper elaborates on the principles and applications of several …
Preparation And Application Of A Polyclonal Antibody To Chlamydomonas Reinhardtii Rsp 10,
2025
College of Food Science and Engineering , Tianjin University of Science and Technology , Tianjin 300457 , China
Preparation And Application Of A Polyclonal Antibody To Chlamydomonas Reinhardtii Rsp 10, Chan Lu, Yuanyuan Zhang, Ye Tao, Zhenchuan Fan, Yanxia Liu
Journal of Food Science and Biotechnology
[Objective ] This study aims to explore the cilium assembly of the radial spoke protein RSP 10 and develop a highly specific polyclonal antibody to RSP 10.[Method ] Firstly,the prokaryotic expression vector pMal-C 2X-rsp10 was constructed,and the expression of MBP-labeled RSP 10 was induced in Escherichia coli BL21 (DE3).After the fusion protein was purified,and the MBP label was excised.The obtained protein was used to immunize New Zealand male white rabbits,and the antibody titer in the anti-serum was determined by indirect ELISA.[Result ] The rabbit anti- Chlamydomonas reinhardtii RSP 10 antibody with high specificity and high sensitivity was obtained after purification …
Fabrication And Antioxidant Property Characterization Of Caffeic Acid Phenethyl Ester-Loaded Nanoparticles,
2025
School of Health Science and Engineering , University of Shanghai for Science and Technology , Shanghai 200093 , China;School of Pharmacy , Shanghai University of Medicine and Health Sciences , Shanghai 201318 , China
Fabrication And Antioxidant Property Characterization Of Caffeic Acid Phenethyl Ester-Loaded Nanoparticles, Yaqiong Chen, Yumeng Li, Hui Wu, Hongdong Song, Xiao Guan
Journal of Food Science and Biotechnology
[Objective ] This study aims to improve the dispersion and utilization of caffeic acid phenethyl ester (CAPE ) in aqueous solutions.[Method ] CAPE-loaded hordein (CAPE-HD ) nanoparticles were constructed via an anti-solvent precipitation method.The physicochemical properties of the nanoparticles were characterized,and the antioxidant properties of the nanoparticles within Caco- 2 cells were investigated.[Result ] The components within the CAPE-HD nanoparticles interacted through non-covalent bonds,such as hydrogen bonding,resulting in an altered state of CAPE upon nanoparticle formation.The CAPE-HD nanoparticles exhibited near-spherical morphology with an average particle size of 193.93 nm,an average polydispersity index (PDI) of 0.26,and average Zeta potential of 14.50 …
Bacterial Diversity In Milk And Traditionally Fermented Dairy Products In Balin Right Banner,
2025
School of Food Science and Engineering , Inner Mongolia Agricultural University , Hohhot 010018 , China
Bacterial Diversity In Milk And Traditionally Fermented Dairy Products In Balin Right Banner, Lijuan Liu, Gaowa Bai, Jinghe Yu, Fang Wang, Youyi Zhang, Li Zhu, Dalai Wuyun
Journal of Food Science and Biotechnology
[Objective ] This study explored the bacterial diversity in milk and traditional fermented dairy products in Balin Right Banner,aiming to lay a foundation for the development and utilization of bacterial resources in the milk and traditional fermented dairy products in this region.[Method ] A total of 32 samples of cow milk,milk tofu,and sour fermentation supernatant of milk tofu were collected from Balin Right Banner,and their bacterial diversity was analyzed by 16S rRNA high-throughput sequencing.[Result ] The diversity and abundance of bacterial communities varied among the milk and traditional fermented dairy products in Balin Right Banner.The dominant bacterial phyla were Firmicutes …
Antimicrobial Resistance And Transmission Risk Of Foodborne Escherichia Coli In Wet Markets,
2025
School of Food Science and Biotechnology , Zhejiang Gongshang University , Hangzhou 310018 , China
Antimicrobial Resistance And Transmission Risk Of Foodborne Escherichia Coli In Wet Markets, Sisi Li, Wei Zhou, Zhijin Zhou, Bin Wang, Chengjun Qiu, Zhangsheng Zhang, Qi Zhang, Yun Sun, Xu Xu, Tao Shen, Jianzhong Han, Daofeng Qu
Journal of Food Science and Biotechnology
[Objective ] To evaluate the antimicrobial resistance and transmission risk of foodborne Escherichia coli in wet markets,this study isolated E.coli from pork,aquatic products,vegetables,and wastewater collected from three wet markets in Hangzhou,Zhejiang.[Method ] The isolates were identified by 16S rRNA sequencing,and their antimicrobial resistance phenotypes were determined via the disk diffusion method.PCR was employed to detect antimicrobial resistance genes and integrons in the isolates.The antimicrobial resistance characteristics of E.coli were analyzed,and conjugation experiments were performed to assess the potential for horizontal transfer of antimicrobial resistance genes.Co-occurrence network analysis was conducted to evaluate the transmission risk of antimicrobial resistance among the isolates.[Result …
A Zif- 8 Electrode Immobilizing Enzyme For Detection Of Glucose Solution In Food,
2025
Key Laboratory of Chemistry and Chemical Engineering on Heavy-Carbon Resources , College of Chemistry and Chemical Engineering , Yili Normal University , Yining 835000 , China
A Zif- 8 Electrode Immobilizing Enzyme For Detection Of Glucose Solution In Food, Tingmei Ma, Shouxin Tang, Wenpan Shi, Yuchen Ma, Haijun Du
Journal of Food Science and Biotechnology
[Objective ] The aim was to construct an electrochemical sensor based on a metal-organic framework-modified electrode for the highly sensitive detection of glucose solution in beverages.[Method ] An electrochemical sensor based on ZIF- 8 composite carbon nanotubes and immobilizing glucose oxidase (GOx ) was prepared by in-situ synthesis and immobilized enzyme technology.The microstructure and morphology of the material were characterized by X-ray diffraction (XRD ),scanning electron microscopy (SEM ),UV-Vis spectroscopy,and infrared spectrometry (IR).The electrochemical performance of the electrode immobilizing enzyme was tested by electrochemical workstation.[Result ] The prepared electrode immobilizing enzyme showed excellent detection performance for glucose solution,with a limit …
