Terrestrial and Aquatic Ecology Commons^™

Are Mussel Shells Environmental Dna Time Capsules? A Comparison Of Extraction Methods For Obtaining Dna From Shell Material., Kelsey Elizabeth Rogers

Online Theses and Dissertations

Freshwater mussels have become some of the most imperiled species in North America and widespread populations have succumbed to pollution and many other anthropogenic-related factors. With molecular techniques evolving, a recent interest in ancient DNA and museum specimens has emerged and prompted a study to test the ability of several extraction methods to isolate DNA from museum mussel specimens. The purpose of this study was to determine if four DNA extraction methods had influence on total DNA yield (ng/mg) from mussel tissue. The hinge ligaments of freshwater mussels ranging in collection date (1984-2015) were used as the source of genetic …

Natural Environmental Gradients Predict The Microhabitat Use, Fine-Scale Distribution, And Abundance Of Three Woodland Salamanders In An Old-Growth Forest, Joseph Alex Baecher

Online Theses and Dissertations

Woodland salamanders (Plethodonidae: Plethodon)－a group of sensitive, direct developing, lungless amphibians－are particularly responsive to gradients in environmental conditions. Because of their functional dominance in terrestrial ecosystems, woodland salamanders are responsible for the transformation of nutrients and translocation of energy between highly desperate levels of trophic organization (detrital food webs and high‐order predators). However, the spatial extent of woodland salamanders' role in the ecosystem is likely contingent upon the distribution of their biomass throughout the forest. Therefore, a better understanding of woodland salamander spatial population dynamics is needed to further understand their role in terrestrial ecosystems. The objectives of this study …

Comparison Of Cytochrome B Variation And Morphological Features Of Isolated Banded Pygmy Sunfish (Elassoma Zonatum) Populations In The Lower Green River And Coastal Plain Systems In Western Kentucky, David Cravens

Online Theses and Dissertations

The banded pygmy sunfish Elassoma zonatum is a wide-ranging species found throughout the Southeastern Coastal Plain of North America. In Kentucky, the populations are found above and below the inner boundary of the Coastal Plain. Due to their geographical separation comparisons in body size and morphological features along with genetic comparisons using mitochondrial cytochrome-b (Cyt-b) were conducted to test relationships between populations. Two sites, Cypress Creek in the Lower Green River system and Rose Creek in the Tradewater River drainage, were sampled from above the inner boundary and two additional sites, Bayou de Chien and Running Slough in the Mississippi …

Sample Collection And Dna Extraction Methods For Environmental Dna Metabarcoding In Headwater Streams, Michael Triston Mullins

Online Theses and Dissertations

DNA is found to be free and ubiquitous in the environment where it is no longer associated with the source organism, and is also known as environmental-DNA (eDNA). Methods optimized for specific environments may be able supplement insight to local taxa richness. With the advent of high throughput sequencing and the proliferation of sequence data in public repositories, insights to the biodiversity of communities at the molecular level have been possible. Thus, this study compared commonly used DNA capture (water precipitation and filtration) and extraction (MoBio's PowerWater, Qiagen's DNeasy Blood and Tissue Kit, and a CTAB protocol) methods in their …

Assessment Of The Macroinvertebrate Assemblages From The Mesohabitats Of A Headwater Stream-Wetland Hydrologic Restoration In Eastern Kentucky, Rebecca Jo Roberts

Online Theses and Dissertations

The bulk of streams in the U.S. have been negatively impacted by anthropogenic disturbances and the streams of Kentucky are no exception. In recent decades stream restoration has become a common practice in order to improve habitat degradation resulting from land use practices such as channelization. Despite the large amount of effort and funding stream restoration projects represent, only a small portion have undergone post-restoration assessments of the ecological response in the restored streams. Slabcamp Creek, a headwater stream located in the Licking River basin in eastern Kentucky, underwent a stream-wetland hydrologic restoration in 2010 in order to improve hydrologic …

Nitrogen Pulses And Competition Between Native And Invasive Plant Species, Nicolas Matallana, Mandy L. Slate, Ragan M. Callaway

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Variation in the timing and size of resource fluctuations can influence how plants grow, allocate biomass, and reproduce. Resources are sometimes made available in relatively continuous, reliable pulses while other times they are temporally separated and unpredictable. Native and invasive plant species are thought to respond differently to resource fluctuations, or pulses, which can influence competitive outcomes. The “Fluctuating Resource Hypothesis” predicts that resource fluctuations benefit invasive species more than native species, potentially because many invaders are highly effective at rapidly capturing resources. In a field setting, we examined the effects of varying nitrogen pulses on competition between exotic invasive …

Redd Habitat Selection For Brook Trout In The Necktie River Bemidji, Mn, William Varela, Zachary Buckley

Journal of Earth and Life Science

The presence of quality suitable habitat is important for the success of naturally reproducing Brook Trout populations. Therefore, measuring redd habitat Brook Trout select for was the focus of this study. Brook Trout redds were observed during the spawning season of 2016. We identified a total of 18 redds in the Necktie River in late October and early November. Specific environmental variables associated with redd selection were measured: velocity, in-stream cover, canopy cover, bank overhang, river width, substrate type and size, temperature, and max-depth. The same environmental variables were measured for 30 randomly selected sites. Redds were distributed in riffles …

Phylogeography And Vicariant Separation Of Two River Darters, Percina Uranidea And Percina Vigil, From Rivers That Drain The North American Interior Highlands, T. Yamashita, J. M. Rigsby, J. Stoeckel

Journal of the Arkansas Academy of Science

The phylogeography and separation of two river darters, Percina uranidea and P. vigil were investigated through sequencing of the mitochondrial Cytochrome B and Cytochrome Oxidase genes. These molecular markers revealed the darters exhibit moderate genetic divergence between two large river drainage systems within the Mississippi River basin associated with the Interior Highlands of midwestern North America. An additional haplotype network analysis also supported these trends. Phylogenetic divergence dating indicated that population separation between the river systems occurred after recent Pleistocene glacial events rather than an early Pleistocene separation.

Human Dimensions Of Habitat Loss In The Plains And Prairie Potholes Ecoregion, Lily A. Sweikert

Electronic Theses and Dissertations

The Plains and Prairie Potholes Ecoregion (PPPE), is located in the north-central contiguous United States and is one of the most imperiled grassland regions in the world. Most of the region is privately owned and used for the production of agricultural commodities. In addition to its direct benefits to humanity, the PPPE provides multiple ecosystem services including, soil and water quality improvements, carbon sequestration, weather amelioration, and wildlife habitat. Recent increases in expansion of row crop agriculture concerns conservation practitioners about the long-term integrity of the PPPE. Conservation practitioners are looking to improve their private landowner conservation initiatives in the …

An Evaluation Of Nitrogen Supplementation And Processed Soy Fractions On The Performance Of Cultured Fishes, Brandon M. White

Electronic Theses and Dissertations

World population growth has resulted in an increased demand for a sustainable food supply. This rapid growth coupled with an increased per capita consumption of seafood, has resulted in many of the world’s marine fisheries being over-exploited in an effort to meet the global demand for seafood. Aquaculture has attempted to fill the supply and demand gap created by the dwindling supply of fish in the world’s oceans. Fishmeal (FM) has traditionally been the primary protein ingredient in aquafeeds fed to farm-raised fish, however its unstable supply and increasing price have driven researchers to identify alternative protein sources. Soybean meal …

Evaluation Of Gamebird Use And Thermal Characteristics Of Alfalfa And Perennial Grasses In Eastern South Dakota, Neal P. Martorelli

Electronic Theses and Dissertations

The northern Great Plains provide critical breeding habitat for waterfowl and gamebirds in the United States. Peak commodity prices in the late 2000s resulted in increased agricultural production and large-scale conversions of grassland habitat to monoculture row crops. However, recent declines in commodity prices have created a renewed interest for private landowners to diversify crops and enroll in government subsidized conservation programs that convert idle grassland and unproductive cropland to wildlife friendly perennial grassland plantings. Exploring alternative grassland restoration techniques can improve the efficiency of management practices to benefit future wildlife habitat and productivity on both public and private lands. …

Effects Of A Large-Scale Habitat Enhancement Project On Largemouth Bass Behavior, Feeding, And Growth In Grand Lake, Texas, Chance Kirkeeng

Electronic Theses and Dissertations

The quality of habitat, or the environment suitable for an organism to survive and reproduce, is often described by the type of features present and the quantity and condition of such features. Habitats in many ecosystems are declining and the loss of important habitats likely affects the success of organisms reliant upon those features. Habitats in reservoir ecosystems are especially at risk because of aging processes that are degrading reservoir habitats at faster rates than habitats in other ecosystems. Habitat enhancement projects are one tool used to combat the effects of aging reservoirs but the benefits of these management actions …

Ecology Of Blanchard Springs Caverns, Ozark National Forest, Arkansas: 42 Years Later, C. J. Midden, S. K. Sasser, J. L. Grove

Journal of the Arkansas Academy of Science

Interrelationships between subterranean and epigean environments affect dispersion and distribution of cave organisms among the macro and microhabitats. This study examined the environmental impact of 42 years of tourism and development in the two lower sections of Blanchard Springs Caverns found in Stone County, Arkansas; and contributes to a better understanding of the seasonal fluctuations of the abiotic and biotic parameters.

Temperature, water quality, and fauna data were collected. A new entrance, lighting, and approximately 12,500 visitors during the 12-month study had no observable effect on cavern temperatures. Stream water quality measurements were comparable to Grove’s 1974 study. Gray bat, …

Response Of Amphibian And Invertebrate Communities To Wetland Mitigation In The Greater Yellowstone Ecosystem, Leah K. Swartz

Graduate Student Theses, Dissertations, & Professional Papers

Wetlands play a critical role in supporting freshwater biodiversity and ecosystem services, but human activities have resulted in large-scale loss and degradation of these habitats across the globe. To offset the decline of wetland area, mitigation wetlands are now frequently constructed, but their ability to replace the functions of natural habitats, including providing habitat for native fauna, remains uncertain. A recent highway reconstruction project in northwestern Wyoming caused impacts to and the destruction of multiple natural wetlands. To mitigate this loss, new wetlands were constructed along the highway corridor. To evaluate the performance of these created wetlands relative to reference …

A Comparison Of Benthic Macroinvertebrate Assemblages Between Perennial And Intermittent Headwater Streams Of The Mattole River In Northern California, Usa, Mason S. London

Cal Poly Humboldt theses and projects

Intermittent streams are common throughout the world and comprise 60% or more of total river lengths in the conterminous United States. Despite their prevalence, intermittent streams are understudied, particularly first-order headwater streams, which are vital for maintaining the function, health and biotic diversity of river networks. In June 2016, I sampled five intermittent and five perennial headwater streams in the Mattole River watershed in northwestern coastal California, USA, to compare benthic macroinvertebrate (BMI) assemblages between intermittent and perennial streams. BMI samples were collected using a 500µm mesh D-net at eight randomly located riffles along a 150-m reach, and then composited, …

Movement, Habitat, And Home Range Of Introduced Bullfrogs (Lithobates Catesbeianus) On Mad River Gravel Ponds (Humboldt Co., Ca, Usa), With Implications For Hydro-Modification As A Method Of Management, Madeline C. Cooper

Cal Poly Humboldt theses and projects

American Bullfrogs are a non-native, invasive species in California (USA), where they are known to have deleterious effects on many native species. Carnivorous adults prey on native amphibians and fish, while herbivorous tadpoles outcompete native tadpoles for algal food resources. Bullfrogs have been successful at colonizing old tailing ponds and other pools left over from mining activities, and these relict pools are common on many rivers in California. Information on the dispersal capabilities of Bullfrogs could help predict range expansions and inform management decisions. Unfortunately, this information is lacking from both their native and invaded range. From May to August …

Modeling The Spread Of Sudden Oak Death Across A Heterogeneous Landscape In Redwood National Park Using A Spatially-Explicit Epidemiological Model, Laura A. Morgan

Cal Poly Humboldt theses and projects

The pathogen Phytophthora ramorum, the causal agent of Sudden Oak Death (SOD), is responsible for the deaths of millions of oak (Quercus spp.) and tanoak (Notholithocarpus densiflorus) trees in California and Oregon (USA). A recent infection in Redwood National Park (RNP) in California (USA) provided an opportunity to adapt an existing SOD model to assess the efficacy of current and proposed management strategies. A common method of SOD treatment includes killing both infected and uninfected hosts in the area of infection, as well as the area surrounding the infection to create buffers to account for undetected …

The Influence Of Canopy Cover And Climate On Early Life-Stage Vital Rates For Northern Red-Legged Frogs (Rana Aurora), And The Implications For Population Growth Rates, Kecly Mcharry

Cal Poly Humboldt theses and projects

Many amphibian species are in decline due to habitat loss and changing climates. Understanding how habitat characteristics and climate influence vital rates, and if they act in concert or in opposition can inform management decisions. This study investigated the potential interaction of canopy cover and climate on early stage vital rates of northern red-legged frogs. Demographic data were collected from sample populations in experimental canopy cover treatments across a latitudinal distribution. Rearing cages were used to estimate hatch success, and mark-recapture surveys to estimate tadpole survival. Ambient air temperature was used as an index of climate because it is easily …

Darlingtonia Californica Phytotelma Organisms: Spatial Relations Within The Metacommunity, Stefani Marie Brandt

Cal Poly Humboldt theses and projects

The California pitcher plant, Darlingtonia californica, provides a unique setting to research community ecology because it harbors small ponds of water, or phytotelmata, in its modified leaves. Each phytotelma hosts a unique community of various invertebrates who live among carcasses of drowned plant prey. These pitchers and their inquiline communities are relatively small yet abundant in the wild, resulting in an ideal natural setup for community composition comparisons. There have been no previous studies that research D. californica inquiline communities across multiple geographic regions concurrently. This study sought to survey phytotelma communities throughout the range of D. californica at …

Coupling Biophysical Complexity And Forest Metabolism In A Floodplain Landscape, Peter Davis

Graduate Student Theses, Dissertations, & Professional Papers

Floodplains are biophysically complex systems that are considered among the most productive and biodiverse ecosystems on Earth. Until recently, quantitative assessment of the relationship between complexity and terrestrial production has been constrained by technological limitation. To address how floodplain biophysical complexity and ecosystem function are related, I employed remote sensing, GIS, and spatial analyses to quantify and couple metrics of complexity and terrestrial production, as well as explore the relationship among complexity, vegetation structural diversity, and terrestrial primary productivity. The study site is a 6.75-km by 1.75-km portion of the Bitterroot River floodplain near Carlton, MT upon which 551 sample …