Other Animal Sciences Commons^™

Genome Editing Using Crispr-Cas9 In Annual Killifish Species, Keria Moritsugu-Vandehey

University Honors Theses

CRISPR-Cas9 genome editing has been used successfully to knock out genes in model organisms such as zebrafish, turquoise killifish, and cichlid fish. CRISPR-Cas9 genome editing has not been verified in the annual killifish, Austrofundulus limnaeus. We hypothesize that targeted editing of the tyrosinase gene in embryos of A. limnaeus will lead to fish without the ability to produce black pigment. Embryos at the 1-cell stage were injected with a Cas9 cocktail containing a mix of guide RNA molecules that target the genomic sequence of the tyrosinase gene and either an mRNA coding for the Cas9 protein or Cas9 protein. …

Physical Properties Of Odorants Affect Behavior Of Trained Detection Dogs During Close-Quarters Searches, Daniel Mejia, Lydia Burnett, Nicholas Hebdon, Peter Stevens, Alexis Shiber, Clay Cranston, Lauryn Degreeff, Lindsay D. Waldrop

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Trained detection dogs have a unique ability to find the sources of target odors in complex fluid environments. How dogs derive information about the source of an odor from an odor plume comprised of odorants with different physical properties, such as diffusivity, is currently unknown. Two volatile chemicals associated with explosive detection, ammonia (NH₃, derived from ammonium nitrate-based explosives) and 2-ethyl-1-hexanol (2E1H, associated with composition C4 plastic explosives) were used to ascertain the effects of the physical properties of odorants on the search behavior and motion of trained dogs. NH₃ has a diffusivity 3.6 times that of …

Impact Of Equine Interaction During Psychotherapy On Anxiety And Depression For Residential Treatment Program Patients Experiencing Substance Withdrawal, Molly M. Friend, Molly C. Nicodemus, Clay Cavinder, Caleb Lemley, Pauline Prince, Katherine A. Cagle-Holtcamp, Rebecca M. Swanson

People and Animals: The International Journal of Research and Practice

As incidences of substance use disorders (SUD) increase in the wake of the COVID-19 pandemic, there is a need for more effective treatment approaches. Further, treatment approaches currently available struggle to retain patients during the period of substance withdrawal in early treatment due to patients’ withdrawal symptoms including increased feelings of anxiety and depression. Withdrawal symptoms have been linked to dysregulated cortisol concentrations present in this period. Psychotherapy incorporating equine interaction (PIE) has emerged in other populations as a treatment that decreases cortisol concentrations and improves treatment retention. The present study investigated the impact of 4 weeks of PIE on …

Animal Conservation In St. Louis, Kate O'Sullivan

Undergraduate Research Symposium

St. Louis has a multitude of organizations involved in the natural sciences. But how many of them actually contribute towards animal conservation? The St. Louis Zoo is an organization that focuses a lot of its effort on presentation, so how does that impact the funds that go towards actually saving the animals? I plan to dive into the different animal-based organizations in St. Louis and its surrounding areas, as well as discuss the positives and negatives of each organization. Furthermore, I will provide examples from several sources that I have been reviewing all year to support my claims. I plan …

Surveys Of Aphonopelma Hentzi In Missouri: Conservation Efforts Through Population, Genetics, And Habitat Studies, Anderson B. Spencer Mr., Becky Hansis-O'Niell

Undergraduate Research Symposium

Aphonopelma hentzi and other tarantula species are greatly understudied organisms. In the Missouri regions there is little knowledge on their mating patterns, gene diversity, or population sizes. The focal glades in this experiment display regional fragmentation, which could prevent tarantulas from traveling and mating between regions of their glades. Due to the lack of knowledge surrounding them, methods for determining gene diversity are harmful to the organism. Our recent work has shown that it is possible to extract genetic information from the molts of burrowing tarantulas. This will allow us to safely determine the lineages and interbreeding patterns of the …

Mark-Recapture Methods In Eastern Diamondback Rattlesnake (Crotalus Adamanteus) Monitoring, Carissa J. Adams

Theses, Dissertations and Capstones

Eastern diamondback rattlesnakes (Crotalus adamanteus; EDB) are an imperiled species native to the Southeastern United States longleaf pine (Pinus palustris) ecosystem. Several markrecapture methods have been used range-wide in hopes of increasing knowledge on their population numbers. I explored the efficacy of two mark-recapture methods (scale cauterization and dorsal head photography) using the Marine Corps Recruit Depot, Parris Island, SC EDB population in this multi-part study. The first method I evaluated was ventral scale branding, which has been widely implemented without knowledge of brand permanence. I classified misidentification errors at capture status (new versus recapture) and …

Greenfeed Methane Measuring Machine, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

The Department of Primary Industries and Regional Development (DPIRD) now has the cutting-edge GreenFeed machine which measures emissions from individual cows and sheep, allowing us to determine Greenhouse Gas (GHG) emissions more accurately in the paddock.

Osteohistological Assessment Of Edmontosaurus Radii From The Liscomb Bonebed, Prince Creek Formation, North Slope, Alaska, Emma Griffin

Student Research Poster Presentations 2024

Hadrosaurs were one of the most diverse dinosaur groups in the Late Cretaceous period and are known for their numerous articulated skeletons and monodominant bonebeds (Wosik et al., 2020). They are more commonly known as duck-billed dinosaurs and are found globally including latitudinal extremes such as the artic and Antarctica. Edmontosaurus, a genus of hadrosaur, are herbivores and are very large growing up to 8,000 pounds. Edmontosaurus were known to travel in herds as a defense mechanism and were one of the last non-avian dinosaurs living during the late Campanian stage through the end of the Maastrichtian stage, right up …

Estimation Of Probability Of Habitat Use Of Roosevelt Elk On The Olympic Peninsula, Vincent Michael Gugliotti

Undergraduate Theses, Professional Papers, and Capstone Artifacts

Estimating the probability of habitat use for a particular species is crucial to the direct management and conservation of that species. Without knowledge of habitat preferences, managers cannot effectively focus efforts on vital resources or landscape types. However, modelling probability of habitat use can be done in several ways which leaves room for variation and uncertainty in the estimates produced by each method. This study is an examination of the variation between two estimates of probability of habitat use while focusing on a particular subspecies of elk that inhabits a unique ecosystem relative to other elk subspecies. I modeled elk …

Using Community Science To Monitor Eastern Box Turtles And Engage Public In Conservation, Sarah Zoppa

Masters Theses

The eastern box turtle (Terrapene carolina carolina; EBT) is a charismatic species with populations in decline due to habitat loss, disease, and other anthropogenic factors. However, due to their cryptic nature, studying an EBT population can be a difficult and intensive process. To investigate EBT populations in Coles County, Illinois, we used a combination of community science and computer-based pattern analysis to develop an approach to tracking individual turtles. Citizen volunteers submitted photos of encountered eastern box turtle carapaces for shell pattern analysis along with GPS coordinates of the encounter. We have received 220 submissions from 47 community volunteers. …

Alpaca Assisted Activities In The New England Alpaca Industry: A Possible Solution To Farm Profitability And Participant Mental Health, Ashley Michele Masse

Honors Theses and Capstones

The high operating costs of the New England alpaca industry were putting the majority of alpaca farmers in this region in a state of financial loss. On a separate note, American communities were experiencing a prevalence of anxiety and depression. What if a single solution could help improve the outcome of both these challenges that seem to have little to do with each other? Alpaca Animal Assisted Activities (alpaca AAAs) was a possible solution. This study explored New England alpaca farm profitability and alpaca AAAs in a way no other research study has done before. A Farmer Survey was mailed …

Alpaca Assisted Activities In The New England Alpaca Industry: A Possible Solution To Farm Profitability And Participant Mental Health, Ashley Michele Masse

Honors Theses and Capstones

The high operating costs of the New England alpaca industry were putting the majority of alpaca farmers in this region in a state of financial loss. On a separate note, American communities were experiencing a prevalence of anxiety and depression. What if a single solution could help improve the outcome of both these challenges that seem to have little to do with each other? Alpaca Animal Assisted Activities (alpaca AAAs) was a possible solution. This study explored New England alpaca farm profitability and alpaca AAAs in a way no other research study has done before. A Farmer Survey was mailed …

The Continuation Of Thoroughbred Racing: A Dual Mission, Isabella V. Williams

Honors Theses and Capstones

Thoroughbred racing is the highest-profile segment of the equine industry in the United States and around the world, accounting for 50% of industry jobs and 25% of industry revenue. Public interest in the sport is rising thanks to social media and celebrity involvement at marquee events like the Kentucky Derby, leading to increased scrutiny of the sport, posing a high risk to human and equine participants alike. Public outcry at equine injuries in particular threatens the sport’s social license to operate (SLO), making it critical to protect the “whole-life welfare” of the animals involved in the sport to ensure its …

Impact Of Short-Distance Road Transportation On Horse Health, Erica Jacquay

Theses and Dissertations--Veterinary Science

Horses are regularly transported across the United States; however, the reasons for travel, trip lengths, and management practices are not well characterized. While long distance transportation has been associated with increased incidences of health-related problems, the impact of short distance transportation on horse health is less understood. Notably, aged horses ( > 15 years) have an altered immune response following short-distance transportation, but this has yet to be compared to young horses. It is also unknown whether transportation stress would alter endocrine responses of horses with metabolic disorders, such as insulin dysregulation (ID) or pituitary pars intermedia dysfunction (PPID).

The objectives …

Determining Population Structure In Kentucky Parascaris Univalens Specimens, Constance Finnerty

Theses and Dissertations--Veterinary Science

Parascaris univalens is a highly pathogenic nematode that affects the gastrointestinal system of young horses. When unaddressed, this parasite causes severe intestinal impactions and even death. Parascaris univalens is also found to have an emergence of anthelmintic resistances, causing a concern for horse owners. Despite its clinical significance, very little work has been done on Parascaris univalens to identify its population structure. This study aimed to determine the population structure of Parascaris univalens through Illumina next generation sequencing (NGS) on three hierarchical categorizations: within a horse, within an isolated herd, and within horses in the state of Kentucky. A total …

Genomic Studies Of The Equine Bloodworm, Strongylus Vulgaris, Nichol Ripley

Theses and Dissertations--Veterinary Science

Strongylus vulgaris is the most pathogenic nematode parasite of grazing horses worldwide. This gastrointestinal parasitic nematode is in the family Strongylidae under the subfamily Strongylinae, or Strongylins. While the epidemiology, complete life cycle, and high pathogenicity of the fourth and fifth larval stages of S. vulgaris are well established, we understand very little about its biology. This parasite is renowned for its unique life cycle and capacity to cause arterial damage, leading to significant health and economic impacts for the equine industry. It also is the most intensively documented Strongylin; however, S. vulgaris has yet to gain drug resistance as …

Gastrointestinal Parasite Prevalence Of Missouri Dogs Against Various Risk Factors, Mattie Cobban

Graduate Theses/Dissertations

Gastrointestinal parasites infect various mammalian species, including dogs, causing symptoms like diarrhea, lethargy, anemia, and possible death. Some gastrointestinal parasites possess zoonotic potential posing risk to dog owners, shelter workers, trainers, veterinary staff, groomers, and the public since dogs have access to public areas such as parks and stores. This study aims to understand the prevalence of gastrointestinal parasites of dogs in southwest Missouri under different environmental conditions. Fecal samples were collected from the Polk County Humane Society, local dog trials, and dog owners. The Mini-FLOTAC fecal flotation method was employed to quantify the type and number of parasite ova …

State Barrier Fence - Esperance Extension Project Overview 2024, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

Western Australia’s State Barrier Fence plays an important role in protecting landholders inside the fence by preventing the movement of animal pests, including wild dogs and emus, from pastoral areas into the agricultural regions. It is a state asset which is managed by the Department of Primary Industries and Regional Development.

The original fences (numbers 1, 2 and 3) were constructed between 1902 and 1907. Originally known as the Rabbit Proof Fences, it has also been known as the State Vermin Fence, the Emu Fence and now the State Barrier Fence. Since 2010, the fence has been upgraded to wild …

Compliance And Roughness In Relation To Gecko Locomotion, Brooklynn Campbell

Williams Honors College, Honors Research Projects

Geckos move efficiently through a variety of substrates, from swaying branches to wooden fences to the sides of structures. Previous studies have studied locomotion and adhesion on manmade and rough substrates; however, the work is limited when discussing locomotion and adhesion as a relationship of compliance and roughness. Ease of movement was quantified by speed, under the assumption that the faster a gecko moves, the easier it is for the gecko to cross the substrate. Gehyra oceanica geckos from Moorea, French Polynesia were tested on substrates varying in compliance and roughness with four different treatments in total. Compliance, roughness, and …

Efficacy Of A Laser As A Starling Deterrent And Its Effect On Lactating Dairy Cow Behavior, Callan A. Lichtenwalter, Marcos Marcondes, Kyle R. Taylor, Craig Mcconnel, Amber Adams Progar

Human–Wildlife Interactions

European starlings (Sturnus vulgaris) cause damage (including structural damage) on dairies, eat cattle feed, and can potentially spread disease through their fecal matter. Deterring starlings from dairies without affecting cow (Bos taurus) welfare is vital, and lasers have been effective starling deterrence tools in urban roosts and in some crops. To evaluate if the use of lasers on dairies would affect starling use of freestall barns as night roosts, or lactating cow behavior, 1 laser was installed in each of the 2 freestall barns at the Knott Dairy Center at Washington State University, Pullman, Washington, USA. …