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Investigating The Role Of Epibiotic Bacteria In Defense Against Chytridiomycosis In The Green Frog, Lithobates Clamitans, Sarah Nalven 2013 Colby College

Investigating The Role Of Epibiotic Bacteria In Defense Against Chytridiomycosis In The Green Frog, Lithobates Clamitans, Sarah Nalven

Honors Theses

Amphibian populations have been declining for several decades, in part due to the emerging fungal skin disease, chytridiomycosis, caused by the chytrid fungus Batrachochytrium dendrobatidis (Bd). Chytridiomycosis has caused extinctions and extirpations in many parts of the world, but its influence varies across species, populations, and individuals. The Green Frog (Lithobates clamitans), which inhabits the eastern half of the United States, seems capable of sustaining Bd infections without experiencing die-offs. It is possible that the Green Frog's epibiotic bacteria are contributing to its defense against Bd as the epibiotic bacteria of several amphibian species are known to play …


Effects Of Endophyte Infected Fescue Alkaloid Ingestion On Energy Metabolism, Nitrogen Balance, In Situ Feed Degradation, And Ruminal Passage Rates, Anne F. Koontz 2013 University of Kentucky

Effects Of Endophyte Infected Fescue Alkaloid Ingestion On Energy Metabolism, Nitrogen Balance, In Situ Feed Degradation, And Ruminal Passage Rates, Anne F. Koontz

Theses and Dissertations--Animal and Food Sciences

The decrease in productivity caused by fescue toxicosis has been estimated to cost the United States livestock industry more than $1 billion per year due to reduced growth and diminished reproductive efficiency. This goal of the research presented in this dissertation is to enhance the knowledge base concerning the underlying physiological changes that occur during fescue toxicosis that lead to reduced intake and weight gain in cattle.

As one of the factors associated with fescue toxicosis is a reduction in feed intake, achieving a consistent and adequate intake of toxins can be a complication. Results from experiment 1demonstrate that ruminal …


Evaluation Of The Effects Of Vitamin K On Growth Performance And Bone Health In Swine, James S. Monegue 2013 University of Kentucky

Evaluation Of The Effects Of Vitamin K On Growth Performance And Bone Health In Swine, James S. Monegue

Theses and Dissertations--Animal and Food Sciences

The role of vitamin K in the blood clotting cascade has been well documented. Vitamin K has recently been implicated in improving bone health. The current studies were conducted to determine the effects of vitamin K in diets with and without mycotoxin contaminated corn on growth performance, bone characteristics, and related blood metabolites in pigs from weaning to market. Menadione sodium bisulfite complex (MSBC, 33% vitamin K) was chosen as the source of supplemental vitamin K because it is the most common form fed to swine. Vitamin K was tested at 0, 0.5, and 2.0 ppm in a corn-soybean meal …


Landscape Characteristics Of Upland Sandpiper Habitat In Michigan, Jacob Lawrence Korte 2013 Wayne State University

Landscape Characteristics Of Upland Sandpiper Habitat In Michigan, Jacob Lawrence Korte

Wayne State University Theses

Grassland bird populations have declined across North America due to habitat loss but at a disproportionately higher rate in the midwestern United States, where extensive coverage of grasslands and other open land ecosystems have been converted to other land cover types. The upland sandpiper (Bartramia longicauda Bechstein, UPSA) is a migratory, area-sensitive, terrestrial shorebird that breeds in grasslands and other open land ecosystem types across their North American range. Although breeding habitats of the Great Plains contain the greatest concentrations of this species, anthropogenic openings such as hayfields and pastures serve as surrogate habitat elsewhere, as do remnant patches …


Effect Of Estradiol Supplementation On Blood Estradiol And Metabolite Levels, And Hepatic Protein Expression, In Growing, Mature, And Senescent Beef Cattle, Edwena D. Miles 2013 University of Kentucky

Effect Of Estradiol Supplementation On Blood Estradiol And Metabolite Levels, And Hepatic Protein Expression, In Growing, Mature, And Senescent Beef Cattle, Edwena D. Miles

Theses and Dissertations--Animal and Food Sciences

Estradiol (Compudose®, COM) implants are extensively used in beef cattle production systems to alter body composition and feed efficiency. Little information exists about the physiological mechanisms affected by COM treatment in growing, mature, and senescent female cattle. Moreover, no reports describe the level of blood estradiol resulting from COM treatment. The effect of COM on levels of plasma estradiol and blood metabolites and proteins, and relative content of glutamine synthetase (GS) and other amino acid nitrogen-metabolizing enzymes in liver tissue, was studied using three experimental models relevant to cow-calf production regimens: senescent cows (Trial 1), young mature (young) versus senescent …


Use Of Mtb-100tm, Provided Through A Mineral Mix, To Reduce Toxicity When Lactating Beef Cows Graze Endophyte-Infected Tall Fescue, Melanie E. Hoar 2013 University of Kentucky

Use Of Mtb-100tm, Provided Through A Mineral Mix, To Reduce Toxicity When Lactating Beef Cows Graze Endophyte-Infected Tall Fescue, Melanie E. Hoar

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted at the University of Kentucky, Eden Shale Farm, Owenton, KY to evaluate the use of MTB-100TM (Alltech, Inc., Nicholasville, KY) to alleviate the symptoms of fescue toxicity when lactating Angus x Beefmaster cows and their calves grazed endophyte-infected KY-31 tall fescue. Experiment 1 provided a carbohydrate based toxin adsorbent, MTB-100TM, ad libitum in a commercial mineral supplement to project a daily consumption rate of 0, 20 or 40 g of MTB-100TM per cow. Cows were weighed, assigned a body condition score (BCS) and hair coat score (HC), rectal temperatures were recorded and …


Detection Of Antibodies Against Parascaris Equorum Excretory-Secretory Antigens, Steffanie V. Burk 2013 University of Kentucky

Detection Of Antibodies Against Parascaris Equorum Excretory-Secretory Antigens, Steffanie V. Burk

Theses and Dissertations--Animal and Food Sciences

Parascaris equorum is a nematode parasite that infects young horses, sometimes causing unthriftiness, respiratory signs, or intestinal impaction in severe cases. Infection can be diagnosed by detection of eggs in feces, but this is only possible after the worms are fully mature. The goal of this study was to develop an antibody-based test for prepatent diagnosis of P. equorum infection. To produce western blot (WB) antigen, P. equorum larvae were cultured for collection of excretory-secretory antigens (ESA). Sera from 18 pregnant broodmares, their subsequent foals, and a group of 12 older mares and geldings were analyzed. In order to check …


Phosphorus Digestibility And Phytate Degradation In Long Yearlings And Mature Horses, Ashley Fowler 2013 University of Kentucky

Phosphorus Digestibility And Phytate Degradation In Long Yearlings And Mature Horses, Ashley Fowler

Theses and Dissertations--Animal and Food Sciences

Much of the phosphorus (P) in grain-based concentrates fed to growing horses is in the form of phytate-P. Little is known about the ability of young horses to degrade phytate-P or whether age affects mineral digestion in horses. The objective of this study was to examine the effect of age on P, calcium (Ca), and magnesium (Mg) digestibility and phytate-P degradation. Four long yearling geldings and 4 mature geldings were fed a diet of alfalfa cubes, timothy cubes and a pelleted concentrate. The diet contained 0.28% total P and 17.4% of that P was in the phytate form. There was …


The Effect Of Pituitary Pars Intermedia Dysfunction On Protein Metabolism And Insulin Sensitivity In Aged Horses, Laurel M. Mastro 2013 University of Kentucky

The Effect Of Pituitary Pars Intermedia Dysfunction On Protein Metabolism And Insulin Sensitivity In Aged Horses, Laurel M. Mastro

Theses and Dissertations--Animal and Food Sciences

Equine pituitary pars intermedia dysfunction (PPID) typically occurs in horses older than 15 years of age and is characterized by hair coat abnormalities, muscle atrophy and decreased insulin sensitivity. The first objective of this research was to compare the rate of whole body protein metabolism and relative abundance of key factors in the signaling pathways associated with muscle protein synthesis and protein breakdown in response to feeding in Control and PPID horses. No differences (P > 0.05) were seen between the PPID and Control groups in whole-body protein metabolism or post-prandial activation of the muscle signaling pathways regulating skeletal muscle protein …


Evidence For The Maturation Of Cellular Immune Responses In Equine Infectious Anemia Virus-Infected Ponies, Chong Liu 2013 University of Kentucky

Evidence For The Maturation Of Cellular Immune Responses In Equine Infectious Anemia Virus-Infected Ponies, Chong Liu

Theses and Dissertations--Veterinary Science

Equine infectious anemia virus (EIAV) has been used as a model to investigate protective mechanisms against lentiviruses. Unlike other lentiviruses, EIAV replication can be eventually controlled in most infected horses leading to an inapparent carrier state free of overt clinical signs which can last for many years. Maintenance of this carrier state is absolutely dependent on active immune responses as evidenced by the fact that immunosuppressive drugs can induce the recurrence of disease. However, the immune mechanisms that are responsible for this control of infection are not yet identified. As the resolution of the initial infection is correlated with the …


Equine Serum Antibody Responses To Streptococcus Equi And Streptococcus Zooepidemicus, Rafaela De Negri 2013 University of Kentucky

Equine Serum Antibody Responses To Streptococcus Equi And Streptococcus Zooepidemicus, Rafaela De Negri

Theses and Dissertations--Veterinary Science

Streptococcus zooepidemicus (Sz) and Streptococcus equi (Se) share 98% DNA sequence homology, but display different pathogenic properties. Infection by one organism does not cross-protect against the other. To better understand pathogenic differences between these organisms and gain information about which proteins are expressed in horses infected experimentally with Se, intrauterine Sz or naturally with respiratory Sz we compared antibody specificities of convalescent sera using ELISA. These comparisons were based on sets of 8 and 14 immunoreactive recombinant proteins of Se strain CF32 and Sz strain NC78, respectively. Sera from donkeys that were previously naturally affected with strangles and later developed …


Development Of A New Allelic Discrimination Real-Time Pcr Assay For The Diagnosis Of Equine Herpesvirus-1 And Characterization Of The Virulence Determinants Of The Virus, Kathryn L. Smith 2013 University of Kentucky

Development Of A New Allelic Discrimination Real-Time Pcr Assay For The Diagnosis Of Equine Herpesvirus-1 And Characterization Of The Virulence Determinants Of The Virus, Kathryn L. Smith

Theses and Dissertations--Veterinary Science

Equine herpesvirus-1 (EHV-1) can cause acute upper respiratory tract disease, abortion, neonatal death and neurological disease in horses. Rapid, accurate and timely diagnosis of EHV-1 infection in horses is important to curtail the spread of this pathogen. It has been reported that the neuropathogenic phenotype of EHV-1 can result from a single non-synonymous nucleotide substitution at position 2254 (A→G2254) in open reading frame 30 (ORF30). This was the basis for the development of an allelic discrimination, real-time PCR assay to distinguish between potential neuropathogenic and non-neuropathogenic EHV-1 strains. However, PCR analysis of a panel of EHV-1 abortion isolates …


Effects Of Grazing Vs. Resting On Soil Chemistry And Plant Community Dynamics For The Central Coast Of California In 2012, Jessica Combs 2012 California Polytechnic State University, San Luis Obispo

Effects Of Grazing Vs. Resting On Soil Chemistry And Plant Community Dynamics For The Central Coast Of California In 2012, Jessica Combs

Animal Science

No abstract provided.


Influence Of Nutritional Modifications On Sow, Litter, And Nursery Performance, Benjamin Edward Bass 2012 University of Arkansas, Fayetteville

Influence Of Nutritional Modifications On Sow, Litter, And Nursery Performance, Benjamin Edward Bass

Graduate Theses and Dissertations

Two separate series of experiments were conducted to determine: 1) the influence of dietary arginine supplementation in late gestation on reproductive performance, and 2) the impact of a whole yeast product in gestation, lactation, and weaned pig diets on reproductive, growth, and immune parameters.

In the first study 99 sows were provided a control diet, or the control diet supplemented with 1% L-arginine from gestation d 93 to 110. Compared to control-fed sows, no differences were observed in reproductive parameters, however a tendency for increased gestational weight gain was observed for sows provided supplemental arginine.

In a second study, 98 …


Timing Of Castration, With Or Without Analgesia, On Growth Performance, Hematology, And Behavior Of Beef Calves, April C. Brown 2012 University of Arkansas, Fayetteville

Timing Of Castration, With Or Without Analgesia, On Growth Performance, Hematology, And Behavior Of Beef Calves, April C. Brown

Graduate Theses and Dissertations

Angus-sired (n = 30) and Hereford-sired (n = 32) bull calves were assigned randomly to 1 of 4 treatments. Treatments were 1) surgical castration near birth, 2) oral administration of the analgesic meloxicam (1 mg/kg of body weight) followed by surgical castration near birth, 3) surgical castration at weaning, or 4) surgical castration at weaning with oral administration of the analgesic meloxicam (1 mg/kg of body weight). Data was recorded for behavior, hematology, and growth at birth and weaning. Calf standing and lying activity was monitored at both stages by recording x and y axis positions of a data logger …


Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2010, Katherine McGraw, Jennie Popp, Wayne Miller 2012 University of Arkansas, Fayetteville

Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2010, Katherine Mcgraw, Jennie Popp, Wayne Miller

AAES Research Reports and Research Bulletins

This report is the seventh in a series of reports examining agriculture’s economic contribution on the Arkansas economy. Utilizing data from the United States Bureau of Economic Analysis (BEA), USDA Economic Research Service (ERS), USDA National Agricultural Statistics Service (NASS), and Minnesota IMPLAN Group, Inc. (MIG), the economic contribution of agriculture on the Arkansas economy was estimated for the most recent year available, 2010. Gross Domestic Product (GDP) by State information for Arkansas in 2010 was compared with those of other states in the Southeast U.S. to give a measure of the relative importance of agriculture in Arkansas.2 The total …


Grey Parrot Number Acquisition: The Inference Of Cardinal Value From Ordinal Position On The Numeral List, Irene M. Pepperberg, Susan Carey 2012 Harvard University

Grey Parrot Number Acquisition: The Inference Of Cardinal Value From Ordinal Position On The Numeral List, Irene M. Pepperberg, Susan Carey

Sentience Collection

No abstract provided.


Haemato Biochemical Alterations In Goats Infected With Coccidiosis, J. Anumol, P. V. Tresamol, K. Vinodkumar, M. R. Saseendranath 2012 Kerala Veterinary and Animal Sciences University

Haemato Biochemical Alterations In Goats Infected With Coccidiosis, J. Anumol, P. V. Tresamol, K. Vinodkumar, M. R. Saseendranath

Biochemistry Collection

Coccidiosis is an important disease which account for anaemia and associated problems like poor growth rate, suppressed resistance and high mortality in kids. While considering the various etiological factors resulting in anaemia among goats of Kerala, coccidiosis account for about 8 per cent, indicating the significance of adopting proper prevention and control measures against this disease. The present study reveals the severity and depth of anaemic changes induced by coccidia in goats. All the haematological parameters, serum biochemical parameters and serum levels of iron, copper and zinc of coccidiosis infected animals are evaluated statistically by comparing with healthy control animals.


Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder 2012 Eastern Illinois University

Intracellular Expression Of An Ice Nucleation Protein Reduces Cryoinjury In Insect Cells, Avril M. Harder

Student Honors Theses

Exposure of insect cells to subzero temperatures typically leads to cell membrane disruption and lethal intracellular ice formation. This study seeks to examine the cryoprotective value of trangenically expressing a bacterial ice nucleation protein (INP) in Spodoptera frugiperda (Sf-21) cells. The bacterium Pseudomonas syringae naturally produces a membrane-bound INP (inaZ), capable of structuring water and initiating ice formation at temperatures as high as -2 °C. I hypothesized that intracellular expression of an altered form of inaZ (PsINP) in Sf-21 cells will mediate highly regulated ice nucleation when cells are cooled to -80 °C in a slow, controlled manner, …


Size And Shape Information Serve As Labels In The Alarm Calls Of Gunnison’S Prairie Dogs Cynomys Gunnisoni, C. N. Slobodchikoff, William R. Briggs, Patricia A. Dennis, Anne-Marie C. Hodge 2012 Northern Arizona University

Size And Shape Information Serve As Labels In The Alarm Calls Of Gunnison’S Prairie Dogs Cynomys Gunnisoni, C. N. Slobodchikoff, William R. Briggs, Patricia A. Dennis, Anne-Marie C. Hodge

Veterinary Science and Medicine Collection

Some animals have the capacity to produce different alarm calls for terrestrial and aerial predators. However, it is not clear what cognitive processes are involved in generating these calls. One possibility is the position of the predator: Anything on the ground receives a terrestrial predator call, and anything in the air receives an aerial predator call. Another possibility is that animals are able to recognize the physical features of predators and incorporate those into their calls. As a way of elucidating which of these mechanisms plays a primary role in generating the structure of different calls, we performed two field …


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