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Effect Of Direct-Fed Microbials And Monensin On In Vitro Rumen Fermentation, Sheryl Wingard 2014 University of Kentucky

Effect Of Direct-Fed Microbials And Monensin On In Vitro Rumen Fermentation, Sheryl Wingard

Theses and Dissertations--Animal and Food Sciences

The impact of supplying a mixed culture of lactate producing bacteria on in-vitro rumen fermentation of forage- (Experiment 1) and concentrate- (Experiment 2) based diets in the presence and absence of monensin was explored. In experiment 1, interactions between DFM and MON were absent (P>0.10) for gas production and fermentative end products. Gas production and fermentative end products were unaffected by DFM alone (P>0.10). Monensin decreased (P<0.001) CH4 and NH3 production, rate and total gas production, as well as total VFA concentration and molar proportions of acetate and butyrate. However, MON increased (P<0.001) proportions of propionate, valerate, isobutyrate and isovalerate. Independently, DFM and MON increased (P<0.001) end point pH. In experiment 2, DFM x MON interactions or tendencies (P=0.07, P<0.01, P<0.01) were present, DFM effects were abated by MON, for rate, total gas production and total VFA concentrations. Acetate:propionate ratio was decreased (P<0.01) with MON and was unaffected by DFM. Ammonia-N concentration was increased (P<0.01) by DFM and unaffected (P=0.75) by MON. Both DFM and MON treatment increased (P<0.01) ruminal pH levels. These studies suggest the effects of DFM and MON of in-vitro fermentation are …


Evaluating Dietary Amino Acid Adequacy In Horses Using Isotopic Techniques, Sara L. Tanner 2014 University of Kentucky

Evaluating Dietary Amino Acid Adequacy In Horses Using Isotopic Techniques, Sara L. Tanner

Theses and Dissertations--Animal and Food Sciences

Little is known about amino acid (AA) requirements in horses despite muscle mass accretion being of importance to an athletic species. Isotope methods for determining AA requirements and whole-body protein synthesis (WBPS) had not been previously used in growing horses. The first study herein was the first to apply isotope methods to determine WBPS in growing horses. In the study, weanling colts received two different levels of crude protein. Whole-body protein kinetics indicated that WBPS was greater when the weanlings were fed the diet with a greater crude protein content (P<0.05). The second study sought to determine a lysine requirement for yearling horses using the indicator AA oxidation (IAAO) method. Despite using six dietary levels of lysine; three above and three below the current recommendation, no breakpoint could be determined. Phenylalanine kinetics were not affected by lysine level (P>0.05), but plasma lysine increased linearly with lysine intake …


Modulation Of Vaccine-Induced Responses By Anthelmintic Treatment In Ponies, Emily Rubinson 2014 University of Kentucky

Modulation Of Vaccine-Induced Responses By Anthelmintic Treatment In Ponies, Emily Rubinson

Theses and Dissertations--Medical Sciences

Vaccines and anthelmintics induce an inflammatory response in equids. Since they are commonly given concurrently, it is practical to study any interaction between them. This study evaluated whether IVM and PYR would modulate the acute phase inflammatory response, the systemic gene expression of pro-inflammatory cytokines, and vaccine-specific titers induced by WNV, EHV, and KLH vaccines. Naturally-infected, yearling ponies were sorted by gender, then fecal epgs. They were randomly assigned to three treatment groups: IVM, PYR, and control. All ponies received vaccinations intramuscularly on days 0 and 29. Whole blood, serum, and plasma samples were collected 1, 3, and 14 days …


Relationships Between Equine Management Practices And Intestinal Parasite Infection, Abigail Pagel 2014 Seattle Pacific University

Relationships Between Equine Management Practices And Intestinal Parasite Infection, Abigail Pagel

Honors Projects

Parasitology is an important area of veterinary medicine, but the risk factors for high parasite loads are not well-understood. Equine intestinal parasites can cause extensive disease and death. In the current study, the relationship between equine intestinal parasite loads and adherence to veterinary guidelines for equine management practices was studied. Satisfactory adherence to guidelines regarding food, pasture, and flooding management was related to lower parasite loads. Adherence to guidelines regarding deworming, quarantine, bedding, and water did not appear to lower parasite loads. Still, adhering to these guidelines has been shown to improve equine welfare, even if they are not related …


Proteomic Analysis Of Mammary Epithelial Cell Development, Alyssa Kay Conly 2014 California Polytechnic State University, San Luis Obispo

Proteomic Analysis Of Mammary Epithelial Cell Development, Alyssa Kay Conly

Master's Theses

In this set of studies, a proteomic approach was used to investigate the protein profile of the mammary epithelial cell (MEC) through different stages of mammary development. The HC11 cell line was used to investigate protein changes between undifferentiated and differentiated MEC, which represent the pregnant and lactating states of the cells. This comparison revealed an interesting differential expression profile underscoring many recognized processes that occur in differentiated MECs, while others unveiled differences between MEC differentiation in vitro and in vivo. Primary MEC were also isolated from virgin, pregnant, and primiparous quiescent mice to compare the virgin state of the …


Comparative Study Of Helminth Parasites Of The Many-Ribbed Salamander, Eurycea Multiplicata And Oklahoma Salamander, Eurycea Tynerensis (Caudata: Plethodontidae), From Arkansas And Oklahoma, A, C. T. McAllister, M. B. Connior, C. R. Bursey, H. W. Robison 2014 Eastern Oklahoma State College

Comparative Study Of Helminth Parasites Of The Many-Ribbed Salamander, Eurycea Multiplicata And Oklahoma Salamander, Eurycea Tynerensis (Caudata: Plethodontidae), From Arkansas And Oklahoma, A, C. T. Mcallister, M. B. Connior, C. R. Bursey, H. W. Robison

Journal of the Arkansas Academy of Science

Ninety many-ribbed salamanders, Eurycea multiplicata and 135 Oklahoma salamanders, Eurycea tynerensis were collected between April 2010 and April 2014 from 14 counties of Arkansas and McCurtain County, Oklahoma (E. multiplicata only) and examined for helminth parasites. Twelve (13%) E. multiplicata were infected, including two (2%) each with Brachycoelium salamandrae, Bothriocephalus rarus, Batracholandros magnavulvaris, Cosmocercoides variabilis, and Omeia papillocauda, and one (1%) each with an oligacanthorhynchid cystacanth and Fessisentis vancleavei. Forty-one (30%) of the E. tynerensis were infected, including seven (5%) with Gorgoderina tenua, two (1%) each with Phyllodistomum solidum and cyclophyllidean tapeworm cysticerci, one (0.7%) with Cylindrotaenia americana, six (3%) …


Natural History Notes And Records Of Vertebrates From Arkansas, M. B. Connior, R. Tumlison, H. W. Robison, C. T. McAllister, D. A. Neely 2014 South Arkansas Community College

Natural History Notes And Records Of Vertebrates From Arkansas, M. B. Connior, R. Tumlison, H. W. Robison, C. T. Mcallister, D. A. Neely

Journal of the Arkansas Academy of Science

No abstract provided.


Behavioural Ecology Of The Black-Flanked Rock-Wallaby (Petrogale Lateralis Lateralis): Refuge Importance In A Variable Environment, Craig Pentland 2014 Edith Cowan University

Behavioural Ecology Of The Black-Flanked Rock-Wallaby (Petrogale Lateralis Lateralis): Refuge Importance In A Variable Environment, Craig Pentland

Theses: Doctorates and Masters

The black-flanked rock-wallaby (Petrogale lateralis lateralis) has suffered a significant decline in its distribution in Western Australia. This has been attributed to introduced predators (predominantly the red fox) and herbivores, fire, and habitat destruction due to clearing. Although since 2001 the Department of Environment and Conservation (DEC) had begun to reintroduce this species back into its former range, little was known of the behavioural ecology of this species. Fox control in the 1980s and 1990s resulted in population increases of rock-wallabies on several reserves in the central wheatbelt of WA. However, recently these populations have rapidly declined despite continuing fox …


Spawning Comparison Of Luxilus Chrysocephalus And Campostoma Oligolepis, Christopher Messer 2014 University of Alabama in Huntsville

Spawning Comparison Of Luxilus Chrysocephalus And Campostoma Oligolepis, Christopher Messer

Summer Community of Scholars Posters (RCEU and HCR Combined Programs)

No abstract provided.


Distribution And Elimination Of 3-Trifluoromethyl-4-Nitrophenol (Tfm) By Sea Lamprey (Petromyzon Marinus) And Non-Target, Rainbow Trout (Oncorhynchus Mykiss) And Lake Sturgeon (Acipenser Fulvescens), Michael W. Le Clair 2014 Wilfrid Laurier University

Distribution And Elimination Of 3-Trifluoromethyl-4-Nitrophenol (Tfm) By Sea Lamprey (Petromyzon Marinus) And Non-Target, Rainbow Trout (Oncorhynchus Mykiss) And Lake Sturgeon (Acipenser Fulvescens), Michael W. Le Clair

Theses and Dissertations (Comprehensive)

The pesticide, 3-trifluoromethyl-4-nitrophenol (TFM), has been highly successful in the control of sea lamprey (Petromyzon marinus) populations in the Great Lakes. Treatments with TFM involve applying it to streams, where it targets larval sea lamprey which live burrowed in the stream substrate. While the toxic mechanism of TFM has been elucidated, and its effects on sea lamprey described, its effects on non-target fish species such as rainbow trout (Oncorhynchus mykiss) and lake sturgeon (Acipenser fulvescens) are not as well understood. The present work demonstrated that rainbow trout show a great capacity to detoxify the lampricide using glucuronidation, when exposed to …


Evaluating The Effect Maturity On The Intake And Digestibility Of Switchgrass Hay Consumed By Beef Steers, David H. Davis 2014 University of Kentucky

Evaluating The Effect Maturity On The Intake And Digestibility Of Switchgrass Hay Consumed By Beef Steers, David H. Davis

Theses and Dissertations--Plant and Soil Sciences

There has been increased interest in utilizing switchgrass (Panicum virgatum) as biomass. There are several challenges to developing this industry, and these have led to the potential use of switchgrass as hay for feeding beef cattle in Kentucky. The effect of increasing maturity on crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and nutritive values of switchgrass hay has been well documented, but few in vivo intake and digestibility trials have been conducted to assess this effect on animal performance when feeding beef cattle. Two in vivo intake and digestibility trials were conducted in 2011 …


Microsporidia Biological Control Agents And Pathogens Of Beneficial Insects, Susan Bjornson, David Oi 2014 Department of Biology, Saint Mary’s University

Microsporidia Biological Control Agents And Pathogens Of Beneficial Insects, Susan Bjornson, David Oi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

No abstract provided.


Humane Education: Perspectives Of Practitioners On Program Evaluation Efforts And Analysis Of Changes In Knowledge, Attitudes, And Empathy In Two Violence Prevention And Intervention Programs, Melanie Wagner 2014 Antioch University - PhD Program in Leadership and Change

Humane Education: Perspectives Of Practitioners On Program Evaluation Efforts And Analysis Of Changes In Knowledge, Attitudes, And Empathy In Two Violence Prevention And Intervention Programs, Melanie Wagner

Antioch University Dissertations & Theses

This descriptive and comparative study examined the current landscape of humane education program evaluation and data analysis through a survey of humane educators across the country. Results of the humane education survey show that data collection and evaluation are occurring in humane education programs but these efforts do not capture and measure empathy, the primary goal of most humane education programs. Humane educators reported they felt the profession is progressive and relevant to a broad host of purposes, from building positive relationships with animals to playing a role in the larger social justice scheme. They also suggested that the field …


Epistasis In Predator-Prey Relationships, Iuliia Inozemtseva 2014 Georgia Southern University

Epistasis In Predator-Prey Relationships, Iuliia Inozemtseva

College of Graduate Studies: Theses & Dissertations

Epistasis is the interaction between two or more genes to control a single phenotype. We model epistasis of the prey in a two-locus two-allele problem in a basic predator- prey relationship. The resulting model allows us to examine both population sizes as well as genotypic and phenotypic frequencies. In the context of several numerical examples, we show that if epistasis results in an undesirable or desirable phenotype in the prey by making the particular genotype more or less susceptible to the predator or dangerous to the predator, elimination of undesirable phenotypes and then genotypes occurs.


Differential Responding By Rhesus Monkeys (Macaca Mulatta) And Humans (Homo Sapiens) To Variable Outcomes In The Assurance Game, Audrey E. Parrish, Sarah F. Brosnan, Bart J. Wilson, Michael J. Beran 2014 Georgia State University

Differential Responding By Rhesus Monkeys (Macaca Mulatta) And Humans (Homo Sapiens) To Variable Outcomes In The Assurance Game, Audrey E. Parrish, Sarah F. Brosnan, Bart J. Wilson, Michael J. Beran

Economics Faculty Articles and Research

Behavioral flexibility in how one responds to variable partner play can be examined using economic coordination games in which subjects play against a variety of partners and therefore may need to alter their behavior to produce the highest payoff. But how do we study this behavioral flexibility once players have settled on a response? Here, we investigated how responding by rhesus monkeys (Macaca mulatta) and humans (Homo sapiens) playing a computerized single-player version of a coordination game, the Assurance game, changed as a function of the variable responses (Stag/Hare) generated by multiple simulations (SIMs). We were interested in whether individuals …


Connecting Model Species To Nature: Predator-Induced Long-Term Sensitization In Aplysia Californica, Maria J. Mason, Amanda J. Watkins, Jordann Wakabayashi, Jennifer Buechler, Christine Pepino, Michelle Brown, William G. Wright 2014 Chapman University

Connecting Model Species To Nature: Predator-Induced Long-Term Sensitization In Aplysia Californica, Maria J. Mason, Amanda J. Watkins, Jordann Wakabayashi, Jennifer Buechler, Christine Pepino, Michelle Brown, William G. Wright

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Previous research on sensitization in Aplysia was based entirely on unnatural noxious stimuli, usually electric shock, until our laboratory found that a natural noxious stimulus, a single sublethal lobster attack, causes short-term sensitization. We here extend that finding by demonstrating that multiple lobster attacks induce long-term sensitization (>= 24 h) as well as similar, although not identical, neuronal correlates as observed after electric shock. Together these findings establish long-and short-term sensitization caused by sublethal predator attack as a natural equivalent to sensitization caused by artificial stimuli.


Four New Mouse Spider Species (Araneae, Mygalomorphae, Actinopodidae, Missulena) From Western Australia, Laura T. Miglio, Danilo Harms, Volker W. Framenau, Mark S. Harvey 2014 University of Western Australia

Four New Mouse Spider Species (Araneae, Mygalomorphae, Actinopodidae, Missulena) From Western Australia, Laura T. Miglio, Danilo Harms, Volker W. Framenau, Mark S. Harvey

Research outputs 2014 to 2021

Four new species of the Mouse Spider genus Missulena Walckenaer, 1805 (family Actinopodidae) are described from Western Australia based on morphological features of adult males. Missulena leniae sp. n. (from the Carnarvon and Yalgoo biogeographic regions), Missulena mainae sp. n. (Carnarvon), Missulena melissae sp. n. (Pilbara) and Missulena pinguipes sp. n. (Mallee) represent a broad spectrum of morphological diversity found in this genus and differ from other congeners by details of the male copulatory bulb, colour patterns, eye sizes, leg morphology and leg spination. Two of the species, M. pinguipes sp. n. and M. mainae sp. n., are characterised by …


Systematics And Biogeography Of The Australian Burrowing Freshwater Crayfish Genus Engaewa Riekk (Decapoda: Parastacidae), Quinton Burnham 2014 Edith Cowan University

Systematics And Biogeography Of The Australian Burrowing Freshwater Crayfish Genus Engaewa Riekk (Decapoda: Parastacidae), Quinton Burnham

Theses: Doctorates and Masters

The overall aim of this study was to explore the systematics and biogeographic patterns of the freshwater crayfish genus Engaewa Riek, a strongly burrowing freshwater crayfish restricted to the coastal corner of south-western Australia (SWA). The genus Engaewa is a Gondwanan relict with great potential as a marker of historical processes, due to its high habitat specificity and low dispersal ability. This study comprises an extensive taxonomic and phylogenetic revision of the genus Engaewa (using both molecular and morphological data), a detailed study of its distribution and uses the knowledge gained to explore biogeographic patterns in the biodiversity hotspot of …


Pcb Effects On Development Of Behavior In Rats, Robert Witte 2014 Bowling Green State University

Pcb Effects On Development Of Behavior In Rats, Robert Witte

Honors Projects

Abstract

PCBs were originally used as industrial chemicals in many devices and applications, including electrical capacitors and transformers, lubricants, and sealants in buildings. PCBs have been investigated and their use has been slowly phased out starting in the U.S. in the 1970s. A worldwide ban was put on the use and manufacturing of PCB in 2001, however they remain in the food chain because of their lipophilic properties and their long half-life. Major sources of PCB include wild-life, fish, and other seafood. Individuals exposed to PCB during development can experience negative effects on neurobiological, cognitive, and behavior functioning in both …


Effects Of Road-Stream Crossings On Populations Of The Idaho Giant Salamander (Dicamptodon Aterrimus), Richard K. Honeycutt 2014 Wildlife Biology

Effects Of Road-Stream Crossings On Populations Of The Idaho Giant Salamander (Dicamptodon Aterrimus), Richard K. Honeycutt

Graduate Student Theses, Dissertations, & Professional Papers

Habitat disturbances affect wildlife populations through numerous mechanisms, and determining specific components of habitat disturbances affecting those populations is challenging. For example, a single disturbance can both change local habitat conditions and impose limitations on dispersal of animals. Both of these components can negatively affect biological responses, such as body condition, local movement patterns, or survival. Culverts are a habitat disturbance having both of these components. Culverts affect local habitat conditions by increasing sediment levels in downstream reaches, negatively affecting animals downstream of culverts. Culverts also impose limits on dispersal by blocking passage of stream organisms to upstream reaches, negatively …


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