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Effect Of Feeding De-Oiled Wet Distillers Grains Plus Solubles On Beef Oxidation And Tenderness, Katherine I. Domenech, Kim A. Varnold, Michelle E. Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson, Chris R. Calkins 2014 University of Nebraska–Lincoln

Effect Of Feeding De-Oiled Wet Distillers Grains Plus Solubles On Beef Oxidation And Tenderness, Katherine I. Domenech, Kim A. Varnold, Michelle E. Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson, Chris R. Calkins

Nebraska Beef Cattle Reports

Cattle fed a de-oiled wet distiller’s grains plus solubles (WDGS) diet were compared to cattle fed corn or traditional (full-fat) WDGS diets to determine effects on discoloration, oxidation, and tenderness of beef aged for seven and 21 days. At seven days of aging, dietary treatment had no effect on discoloration. At 21 days of aging, beef from cattle fed de-oiled WDGS had less oxidation than the corn control and several of the full-fat WDGS treatments. Although tenderness increased with aging and retail display, dietary treatment had no effect on tenderness. These findings suggest that these dietary treatments, followed by a …


Effect Of Feeding Different Types Of Byproducts And Concentrations Throughout A Beef Growing System On Ground Beef Color And Lipid Oxidation, Joe O. Buntyn, Brandy D. Cleveland, Amy L. Redfield Redfield, James C. MacDonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan 2014 University of Nebraska–Lincoln

Effect Of Feeding Different Types Of Byproducts And Concentrations Throughout A Beef Growing System On Ground Beef Color And Lipid Oxidation, Joe O. Buntyn, Brandy D. Cleveland, Amy L. Redfield Redfield, James C. Macdonald, Galen E. Erickson, Tommi F. Jones, Ty B. Schmidt, Gary A. Sullivan

Nebraska Beef Cattle Reports

The objective of this trial was to evaluate the effect of feeding different concentrations of wet distillers grains during winter backgrounding and either modified wet distillers grains or Sweet Bran® during the finishing phase on ground beef color and lipid oxidation. After a 14 day aging period, ground beef patties were made and placed in a simulated retail display for seven days. There were no overall differences in lipid oxidation between treatments but was a treatment by day interaction for discoloration. Ground beef from heifers finished with modified wet distillers grains discolored at a greater extent when compared to …


Shelf Life Of Cooked Ground Beef Patties From Cattle Fed Wet Distillers Grains With Solubles, Nathan T. Dierks, Tommi F. Jones, Kimberly A. Varnold Varnold, Derek J. Schroeder, Amy L. Redfield, Gary A. Sullivan 2014 University of Nebraska–Lincoln

Shelf Life Of Cooked Ground Beef Patties From Cattle Fed Wet Distillers Grains With Solubles, Nathan T. Dierks, Tommi F. Jones, Kimberly A. Varnold Varnold, Derek J. Schroeder, Amy L. Redfield, Gary A. Sullivan

Nebraska Beef Cattle Reports

Cattle were grazed without or with energy supplementation of wet distillers grains with solubles (WDGS) during backgrounding, and were finished on a corn-based diet with or without 35% WDGS. Ground beef patties were made from shoulder clods, cooked, and stored in a refrigerated or frozen state. Cattle supplemented with WDGS had greater lipid oxidation in cooked ground beef patties regardless of finishing diet or storage type.


Differences In Fecal Bacterial Community Composition Between Beef Steers Which Are High-Shedders And Low-Shedders Of Shiga Toxin-Producing Escherichia Coli (Stec), Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando 2014 University of Nebraska–Lincoln

Differences In Fecal Bacterial Community Composition Between Beef Steers Which Are High-Shedders And Low-Shedders Of Shiga Toxin-Producing Escherichia Coli (Stec), Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando

Nebraska Beef Cattle Reports

The community composition of the fecal microbiota was compared between beef steers which were high-shedders and low-shedders of Shiga toxin-producing Escherichia coli. Based on Shannon and Chao 1 diversity indices, the high-shedders had a more diverse fecal bacterial community than the low-shedding steers. Members of the genus Prevotella were observed as being more abundant in the low-shedders compared to the high-shedders, while Succinivibrio were more abundant in the high-shedders. Isolation of specific bacteria which are significantly more abundant in low-shedders may pave the way to developing direct-fed microbials which are effective in reducing STEC shedding among high-shedding beef steers.


Optimal Marketing Date Of Steers Depends On Marketing Strategy, James C. MacDonald, Cody J. Schneider, Kelsey M. Rolfe Rolfe, Stephen D. Kachman, Terry J. Klopfenstein, Galen E. Erickson 2014 University of Nebraska–Lincoln

Optimal Marketing Date Of Steers Depends On Marketing Strategy, James C. Macdonald, Cody J. Schneider, Kelsey M. Rolfe Rolfe, Stephen D. Kachman, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Seven research trials conducted over five years at the University of Nebraska–Lincoln were summarized to determine how animal performance changes through the finishing period on a live and carcass weight basis. Live weight, carcass weight, carcass ADG, and carcass feed efficiency all changed quadratically throughout the feeding period; live ADG and live feed efficiency declined linearly. During times of negative profit margins, optimal profitability for steers marketed on a live-basis occurred by selling early, whereas optimal profitability was achieved by feeding steers marketed on a carcass-basis longer.


The Effects Of Diet And Cooler Aging On Specific Flavor Notes In Beef, Kimberly A. Varnold, Chris R. Calkins, Rhonda K. Miller Miller, Galen E. Erickson 2014 University of Nebraska–Lincoln

The Effects Of Diet And Cooler Aging On Specific Flavor Notes In Beef, Kimberly A. Varnold, Chris R. Calkins, Rhonda K. Miller Miller, Galen E. Erickson

Nebraska Beef Cattle Reports

Crossbred steers (n = 64) were grazed on warm- or cool-season grass-dominated pastures, without or with energy supplementation of wet distillers grains with solubles (WDGS), and were finished on a corn-based diet with or without 35% WDGS. Finishing on corn increased desirable flavor notes and decreasedundesirable flavor notes in both L. dorsi and B. femoris steaks. In addition, grazing on warm-season grasses increased the prevalence of undesirable flavors but was often dissipated by the addition WDGS supplementation. Longer aging periods tended to increase the prevalence of undesirable flavors, especially in B. femoris steaks. It is recommended producers provide WDGS supplementation, …


Use Of Robust Multivariate Linear Mixed Models For Estimation Of Genetic Parameters For Carcass Traits In Beef Cattle, S. O. Peters, K. Kizilkaya, D. J. Garrick, R. L. Fernando, E. J. Pollak, R. Mark Enns, M. De Donato, O. O. Ajayi, I. G. Imumorin 2014 Berry College

Use Of Robust Multivariate Linear Mixed Models For Estimation Of Genetic Parameters For Carcass Traits In Beef Cattle, S. O. Peters, K. Kizilkaya, D. J. Garrick, R. L. Fernando, E. J. Pollak, R. Mark Enns, M. De Donato, O. O. Ajayi, I. G. Imumorin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Assumptions of normality of residuals for carcass evaluation may make inferences vulnerable to the presence of outliers, but heavy-tail densities are viable alternatives to normal distributions and provide robustness against unusual or outlying observations when used to model the densities of residual effects. We compare estimates of genetic parameters by fitting multivariate Normal (MN) or heavy-tail distributions (multivariate Student’s t and multivariate Slash, MSt and MS) for residuals in data of hot carcass weight (HCW), longissimus muscle area (REA) and 12th to 13th rib fat (FAT) traits in beef cattle using 2475 records from 2007 to 2008 from a large …


Beef Cattle Body Temperature During Climatic Stress: A Genome-Wide Association Study, Jeremy T. Howard, Stephen D. Kachman, Warren M. Snelling, E. John Pollak, Daniel C. Ciobanu, Larry A. Kuehn, Matthew L. Spangler 2013 University of Nebraska-Lincoln

Beef Cattle Body Temperature During Climatic Stress: A Genome-Wide Association Study, Jeremy T. Howard, Stephen D. Kachman, Warren M. Snelling, E. John Pollak, Daniel C. Ciobanu, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Cattle are reared in diverse environments and collecting phenotypic body temperature (BT) measurements to characterize BT variation across diverse environments is difficult and expensive. To better understand the genetic basis of BT regulation, a genome-wide association study was conducted utilizing crossbred steers and heifers totaling 239 animals of unknown pedigree and breed fraction. During predicted extreme heat and cold stress events, hourly tympanic and vaginal BT devices were placed in steers and heifers, respect i v e l y. I n d i v i dua l s we r e genotyped with t h e BovineSNP50K_v2 assay and data …


Atf3 Expression In The Corpus Luteum: Possible Role In Luteal Regression, Dagan Mao, Xiaoying Hou, Heather Talbott, Robert A. Cushman, Andrea S. Cupp, John S. Davis 2013 University of Nebraska Medical Center

Atf3 Expression In The Corpus Luteum: Possible Role In Luteal Regression, Dagan Mao, Xiaoying Hou, Heather Talbott, Robert A. Cushman, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

The present study investigated the induction and possible role of activating transcription factor 3 (ATF3) in the corpus luteum. Postpubertal cattle were treated at midcycle with prostaglandin F2α(PGF) for 0–4 hours. Luteal tissue was processed for immunohistochemistry, in situ hybridization, and isolation of protein and RNA. Ovaries were also collected from midluteal phase and first-trimester pregnant cows. Luteal cells were prepared and sorted by centrifugal elutriation to obtain purified small (SLCs) and large luteal cells (LLCs). Real-time PCR and in situ hybridization showed that ATF3 mRNA increased within 1 hour of PGF treatment in vivo. Western blot and immunohistochemistry demonstrated …


Loss Of Vascular Endothelial Growth Factor A (Vegfa) Isoforms In The Testes Of Male Mice Causes Subfertility, Reduces Sperm Numbers, And Alters Expression Of Genes That Regulate Undifferentiated Spermatogonia, Ningxia Lu, Kevin M. de Avila, Debra T. Clopton, William E. Pohlmeier, Vanessa M. Brauer, Renee M. McFee, John S. Weber, Napoleone Ferrara, David W. Silversides, Andrea S. Cupp 2013 University of Nebraska-Lincoln

Loss Of Vascular Endothelial Growth Factor A (Vegfa) Isoforms In The Testes Of Male Mice Causes Subfertility, Reduces Sperm Numbers, And Alters Expression Of Genes That Regulate Undifferentiated Spermatogonia, Ningxia Lu, Kevin M. De Avila, Debra T. Clopton, William E. Pohlmeier, Vanessa M. Brauer, Renee M. Mcfee, John S. Weber, Napoleone Ferrara, David W. Silversides, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Vascular endothelial growth factor A (VEGFA) isoform treatment has been demonstrated to alter spermatogonial stem cell homeostasis. Therefore, we generated pDmrt1-Cre;Vegfa−/− (knockout, KO) mice by crossing pDmrt1-Cre mice to floxed Vegfa mice to test whether loss of all VEGFA isoforms in Sertoli and germ cells would impair spermatogenesis. When first mated, KO males took 14 days longer to get control females pregnant (P < .02) and tended to take longer for all subsequent parturition intervals (9 days; P < .07). Heterozygous males sired fewer pups per litter (P < .03) and after the first litter took 10 days longer (P < .05) to impregnate females, suggesting a more progressive loss of fertility. Reproductive organs were collected from 6-month-old male mice. There were fewer sperm per tubule in the corpus epididymides (P < .001) and fewer ZBTB16-stained undifferentiated spermatogonia (P < .003) in the testes of KO males. Testicular mRNA abundance for Bcl2 (P < .02), Bcl2:Bax (P < .02), Neurog3 (P < .007), and Ret was greater (P = .0005), tended to be greater for Sin3a and tended to be reduced for total Foxo1 (P < .07) in KO males. Immunofluorescence for CD31 and VE-Cadherin showed no differences in testis vasculature; however, CD31-positive staining was evident in undifferentiated spermatogonia only in KO testes. Therefore, loss of VEGFA isoforms in Sertoli and germ cells alters genes necessary for long-term maintenance of undifferentiated spermatogonia, ultimately reducing sperm numbers and resulting in subfertility.


The Color And Consistency Of Steaks And Ground Beef Produced From Mature Bulls, Jace Joseph Hollenbeck 2013 University of Arkansas, Fayetteville

The Color And Consistency Of Steaks And Ground Beef Produced From Mature Bulls, Jace Joseph Hollenbeck

Graduate Theses and Dissertations

Two experiments were conducted to compare the color and cooked attributes of steaks and ground beef produced from mature bulls. Beef from mature bulls was used to test the effects of lactic acid (LA) enhancement solution pH (2.5, 3.0, or 3.5; B25, B30, B35, respectively) on fresh (5 d of display) and cooked color and tenderness of strip loin steaks (Exp. 1) and the effect of high pH trim on precooked ground beef patties (Exp. 2). In experiment 1, mature bull strip steaks were enhanced to 111% with a LA, sodium bicarbonate, and tap water solution, in comparison to USDA …


Genome-Wide Prediction Of Age At Puberty And Reproductive Longevity In Sows, Julie Kathleen Tart, Rodger K. Johnson, Justin W. Bundy, N. N. Ferdinand, A. M. McKnite, Jennifer R. Wood, Phillip S. Miller, M. F. Rothschild, Matthew L. Spangler, Dorian J. Garrick, Stephen D. Kachman, Daniel C. Ciobanu 2013 University of Nebraska-Lincoln

Genome-Wide Prediction Of Age At Puberty And Reproductive Longevity In Sows, Julie Kathleen Tart, Rodger K. Johnson, Justin W. Bundy, N. N. Ferdinand, A. M. Mcknite, Jennifer R. Wood, Phillip S. Miller, M. F. Rothschild, Matthew L. Spangler, Dorian J. Garrick, Stephen D. Kachman, Daniel C. Ciobanu

Department of Animal Science: Faculty Publications

Traditional selection for sow reproductive longevity is ineffective due to low heritability and late expression of the trait. Incorporation of DNA markers into selection programs is potentially a more practical approach for improving sow lifetime productivity. Using a resource population of crossbred gilts, we explored pleiotropic sources of variation that influence age at puberty and reproductive longevity. Of the traits recorded before breeding, only age at puberty significantly affected the probability that females would produce a first parity litter. The genetic variance explained by 1-Mb windows of the sow genome, compared across traits, uncovered regions that influence both age at …


Using High-Fiber Diets To Limit Energy Intake In Developing Gilts: Effects On Puberty, Reproduction, Culling Rates, Lifetime Productivity, And Progeny Health And Growth, Phillip S. Miller, Rodger Johnson, Justin Bundy, Daniel Ciobanu 2013 University of Nebraska - Lincoln

Using High-Fiber Diets To Limit Energy Intake In Developing Gilts: Effects On Puberty, Reproduction, Culling Rates, Lifetime Productivity, And Progeny Health And Growth, Phillip S. Miller, Rodger Johnson, Justin Bundy, Daniel Ciobanu

Department of Animal Science: Faculty Publications

A total of 182 gilts were used to evaluate the effects of limiting energy intake during development compared to traditional corn-soybean meal based diets using the addition of soybean hulls to reduce caloric intake with ad libitum access to feed. The gilt developmental period consisted of 110 days, from 120 to 230 days of age. Data collected during development included body weight, backfat, longissimus muscle area, and feed intake, every 2 weeks. Estrus detection was conducted on a daily basis until breeding. Females were maintained in the breeding groups for 4 parities (785 days of age), at which time data …


Genome-Wide Association Analyses Of Sow Reproduction And Lifetime Productivity, Daniel Ciobanu 2013 University of Nebraska-Lincoln

Genome-Wide Association Analyses Of Sow Reproduction And Lifetime Productivity, Daniel Ciobanu

Department of Animal Science: Faculty Publications

Concurrent with increased prolificacy, high sow death losses and replacement rates are serious economic and welfare issues facing producers. Reproductive failure is the most frequent reason for culling sows. Lifetime productivity is characterized by moderate heritability and high phenotypic variance and, as a result, substantial genetic variation is expected to exist in most populations. Traditional selection for sow reproductive longevity results is ineffective due to low heritability and late expression of the trait. The primary goal of this research project was to identify DNA markers associated with reproductive and sow lifetime productivity that can be used in nucleus herds to …


Nebraska Bqa: Herd Health Plan/Protocol, Rob Eirich 2013 University of Nebraska-Lincoln

Nebraska Bqa: Herd Health Plan/Protocol, Rob Eirich

Nebraska Beef Quality Assurance Program

Nebraska cattlemen have demonstrated a commitment to the integrity of today's beef products by implementing sound cattle management practices. Consumers are looking for beef raised in a healthy and safe environment. An appropriate Herd Health Plan/Protocol ensures that all cattle are raised in the best health.


Nebraska Bqa: Veterinarian Client Patient Relationship, Rob Eirich 2013 University of Nebraska-Lincoln

Nebraska Bqa: Veterinarian Client Patient Relationship, Rob Eirich

Nebraska Beef Quality Assurance Program

Beef producers are committed producing a high quality, safe and wholesome beef product. A commitment to herd health and veterinary input is critical to achieving this goal. To this end, developing a veterinarian client-patient relationship (VCPR) is important and will benefit all aspects of cattle performance. Veterinarians' working understanding of cattle disease and parasites can help cattle raisers develop targeted vaccination and treatment programs.


Effect Of Citrus Byproducts On Survival Of O157:H7 And Non-O157 Escherichia Coli Serogroups Within In Vitro Bovine Ruminal Microbial Fermentations, Heather A. Duoss-Jennings, Ty B. Schmidt, Todd R. Callaway, Jeffery A. Carroll, James M. Martin, Sara A. Shields-Menard, Paul R. Broadway, Janet R. Donaldson 2013 Iowa State University

Effect Of Citrus Byproducts On Survival Of O157:H7 And Non-O157 Escherichia Coli Serogroups Within In Vitro Bovine Ruminal Microbial Fermentations, Heather A. Duoss-Jennings, Ty B. Schmidt, Todd R. Callaway, Jeffery A. Carroll, James M. Martin, Sara A. Shields-Menard, Paul R. Broadway, Janet R. Donaldson

Department of Animal Science: Faculty Publications

Citrus byproducts (CBPs) are utilized as a low cost nutritional supplement to the diets of cattle and have been suggested to inhibit the growth of both Escherichia coli O157:H7 and Salmonella. The objective of this study was to examine the effects in vitro that varying concentrations of CBP in the powdered or pelleted variety have on the survival of Shiga-toxin Escherichia coli (STEC) serotypes O26:H11, O103:H8, O111:H8, O145:H28, and O157:H7 in bovine ruminal microorganism media. The O26:H11, O111:H8, O145:H28, and O157:H7 serotypes did not exhibit a change in populations in media supplemented with CBP with either variety. The O103:H8 …


The Effect Of Soluble Uterine Factors On Porcine Embryo Development Within A Three-Dimensional Alginate Matrix System, Catherine Sargus-Patino, Elane C. Wells, Jeremy R. Miles, Angela K. Pannier 2013 University of Nebraska-Lincoln

The Effect Of Soluble Uterine Factors On Porcine Embryo Development Within A Three-Dimensional Alginate Matrix System, Catherine Sargus-Patino, Elane C. Wells, Jeremy R. Miles, Angela K. Pannier

Department of Animal Science: Faculty Publications

Between day 10 and 12 of gestation in the pig, the embryo undergoes a dramatic morphological change, known as elongation. During elongation the embryo produces and secretes estrogen, which serves as a key signal for maternal recognition of pregnancy. The uterine environment prepares for embryo elongation and implantation by releasing nutrients, metabolites, and hormones in a time-dependent manner relative to paracrine signals from the conceptus (i.e., estrogen) and endocrine/paracrine signals within the endometrium (i.e., progesterone). Despite advances in identification of specific uterine factors present throughout pregnancy in the pig, little is known about the exact mechanisms by which porcine embryos …


Genetic Testing For Tmem154 Mutations Associated With Lentivirus Susceptibility In Sheep, Michael P. Heaton, Theodore S. Kalbfleisch, Dustin T. Petrik, Barry Simpson, James W. Kijas, Michael L. Clawson, Carol G. Chitko-McKown, Gregory P. Harhay, Kreg A. Leymaster, International Sheep Genomics Consortium 2013 US Meat Animal Research Center, Clay Center, NE

Genetic Testing For Tmem154 Mutations Associated With Lentivirus Susceptibility In Sheep, Michael P. Heaton, Theodore S. Kalbfleisch, Dustin T. Petrik, Barry Simpson, James W. Kijas, Michael L. Clawson, Carol G. Chitko-Mckown, Gregory P. Harhay, Kreg A. Leymaster, International Sheep Genomics Consortium

Roman L. Hruska U.S. Meat Animal Research Center: Reports

In sheep, small ruminant lentiviruses cause an incurable, progressive, lymphoproliferative disease that affects millions of animals worldwide. Known as ovine progressive pneumonia virus (OPPV) in the U.S., and Visna/Maedi virus (VMV) elsewhere, these viruses reduce an animal’s health, productivity, and lifespan. Genetic variation in the ovine transmembrane protein 154 gene (TMEM154) has been previously associated with OPPV infection in U.S. sheep. Sheep with the ancestral TMEM154 haplotype encoding glutamate (E) at position 35, and either form of an N70I variant, were highly-susceptible compared to sheep homozygous for the K35 missense mutation. Our current overall aim was to characterize TMEM154 in …


Nutritional Management Post-Ai To Enhance Pregnancy Outcomes, S. L. Lake, R. Arias, P. Gunn, G. A. Bridges 2013 University of Wyoming

Nutritional Management Post-Ai To Enhance Pregnancy Outcomes, S. L. Lake, R. Arias, P. Gunn, G. A. Bridges

Range Beef Cow Symposium

Effective replacement heifer development is a critical segment of the integrated management program in an efficient beef cow production system. The most critical factor determining the success of any heifer development program is nutrition. Most efforts are concentrated in providing the right amount and quality of feed to achieve gains from 1.5 to 2.0 lb/day, so heifers can reach 65% of their mature BW by the day of breeding. Approximately 80% of the U.S. cow-herds are spring calving, which means that producers utilizing estrous synchronization and AI are keeping their heifers in a feedlot environment until heifers are inseminated. Immediately …


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