Meat Science Commons^™

Genetic Parameter Estimates For Calving Difficulty And Birth Weight In A Multibreed Population, Cashley M. Ahlberg, Larry A. Kuehn, R. Mark Thallman Thallman, Stephen D. Kachman, Matthew L. Spangler

Nebraska Beef Cattle Reports

Eighteen breeds were utilized to estimate genetic parameters for birth weight and calving difficulty on first-parity females. Birth weight and calving difficulty were moderately heritable allowing for genetic selection to decrease calving difficulty. Genetic correlation estimates were positive between direct effects for birth weight and calving difficulty. This work will serve as the foundation for estimating across-breed EPD for calving difficulty in the U.S.

Comparison Of Wet Or Dry Distillers Grains Plus Solubles To Corn As An Energy Source In Forage-Based Diets, Nerissa A. Ahern, Brandon L. Nuttelman, Terry J. Klopfenstein Klopfenstein, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Four experiments were conducted comparing wet or dry distillers grains plus solubles to each other or to corn as an energy source in forage-based diets. Diets included dry distillers grains plus solubles, wet distillers grains plus solubles or dry-rolled corn, with sorghum silage, grass hay and supplement. Data were pooled to generate ADG at differing inclusions allowing energy value of wet distillers grains plus solubles to be calculated relative to dry-rolled corn. The energy value of distillers grains plus solubles fed at 15% of diet DM was 137% and fed at 30% of the diet DM was 136% relative to …

Impact Of Algae Supplemented Diets Combined With Antioxidants On The Nutritional Profile, Quality Attributes, And Storage Stability Of Chicken Breast Meat, Rebecca G. Norcross

Theses and Dissertations--Animal and Food Sciences

Consumers’ demands for ω-3 polyunsaturated fatty acids (PUFAs) are at all-time high. Algae, a common source of PUFAs, and antioxidants are both used as supplements in livestock feeds, are known to affect the overall quality of meat. To implement PUFA deposits into broiler meat, this study evaluated combining antioxidants and algae in broiler feed to enhance the breast meat quality. Broilers were fed diets supplemented with 50 IU Vitamin E or 200 g/ton EconomasE (EcoE, an antioxidant pack) plus 10 IU Vitamin E, with or without 0.5% algae extract (SP-1). The feed oil was partially oxidized soybean oil (POV: 86 …

Dehydrated Citrus Pulp Alters Feedlot Performance Of Crossbred Heifers During The Receiving Period And Modulates Serum Metabolite Concentrations Before And After An Endotoxin Challenge, J. T. Cribbs, B. C. Bernhard, T. R. Young, M. A. Jennings, N.C. Burdick Sanchez, J. A. Carroll, T. R. Callaway, T. B. Schmidt, B. J. Johnson, R. J. Rathmann

Department of Animal Science: Faculty Publications

English × Continental heifers (n = 180) were sourced in 2 loads (219.3 ± 16.0 and 221.4 ± 16.4 kg, respectively) from commercial auction barns to study the effects of feeding dehydrated citrus pulp (DCP) on feedlot performance of newly received heifers. A completely randomized block design was used with BW nested within arrival load and blocked by BW into 3 dietary treatments (36 pens, 5 heifers/ pen, 12 blocks, 3 pens/block, and 12 pens/treatment). Treatment diets contained 1) 0% DCP (control diet [CON]), 2) 10% DCP, or 3) 20% DCP on a DM basis. Diets containing DCP were exchanged …

Case Study: Producer Concerns And Perceptions Regarding The Effect Of Methane On Cattle Production And The Environment: A Survey Of Nebraska Producers, B. M. Boyd, A. Jones, Lisa Franzen-Castle, K. Jenkins, Richard J. Rasby, Matt K. Luebbe, Richard R. Stowell, Samodha C. Fernando, Galen E. Erickson

Department of Animal Science: Faculty Publications

Enteric methane production from cattle and its effect on climate change has been a topic of debate. Multiple studies have explored methods to reduce cattle enteric methane production while simultaneously improving performance. However, most strategies developed have not been widely implemented by cattle producers. Knowledge of producer concerns and perceptions on methane production from cattle and its effect on the environment may be limited. Therefore, the objectives of this survey were to determine what Nebraska producers know about methane production by cattle and how it affects performance and to determine whether different age groups, regions of Nebraska, and production size …

Development Of A Row-Crop Tractor Data Acquisition System, James Roeber, Santosh Pitla, Roger M. Hoy, Joe D. Luck

Department of Animal Science: Faculty Publications

Numerous advancements in machinery performance on row-crop agricultural tractors have been made in recent years. Currently, the Organisation for Economic Co-operation and Development (OECD) only tests predetermined points (E.g., max. power, max. torque) for drawbar and hydraulic power. This method of testing has been used for many years with the tractor at a steady state which is usually not typical of row-crop operations. A data acquisition system (DAQ) was designed that records implement energy consumption (E.g., drawbar loading, PTO power, and hydraulic power). The system used a LabVIEW program and a compact data acquisition system (cDAQ) to record data from …

An Application Of Mesh Enrichment Analysis In Livestock, Gota Morota, F. Moreno, James L. Petersen, Daniel C. Ciobanu, K. Tsuyuzaki, I. Nikaido

Department of Animal Science: Faculty Publications

An integral part of functional genomics studies is to assess the enrichment of specific biological terms in lists of genes found to be playing an important role in biological phenomena. Contrasting the observed frequency of annotated terms with those of the background is at the core of overrepresentation analysis (ORA). Gene Ontology (GO) is a means to consistently classify and annotate gene products and has become a mainstay in ORA. Alternatively, Medical Subject Headings (MeSH) offers a comprehensive life science vocabulary including additional categories that are not covered by GO. Although MeSH is applied predominantly in human and model organism …

Optimizing The Use Of Fibrous Residues In Beef And Dairy Diets, James C. Macdonald, G. E. Erickson, P. J. Kononoff, T. J. Klopfenstein

Department of Animal Science: Faculty Publications

Increased corn prices over the past decade have altered land use away from traditional forage in favor of corn. Accordingly, beef and dairy producers have had to adopt non-traditional forage resources into their production systems, many of which have become available as a result of increased corn production. Byproducts of the wet and dry milling industries have been used to replace both corn and forage in beef and dairy diets. Byproducts containing corn bran have large amounts of readily digestible hemicellulose. The use of byproducts may increase milk production, ADG, and G:F in dairy, beef growing, and beef finishing diets, …

Microbial Diversity And Activity In The Nematostella Vectensis Holobiont: Insights From 16s Rrna Gene Sequencing, Isolate Genomes, And A Pilot-Scale Survey Of Gene Expression, Jia Y. Har, Tim Helbig, Ju H. Lim, Samodha C. Fernando, Adam M. Reitzel, Kevin Penn, Janelle R. Thompson

Department of Animal Science: Faculty Publications

We have characterized the molecular and genomic diversity of the microbiota of the starlet sea anemone Nematostella vectensis, a cnidarian model for comparative developmental and functional biology and a year-round inhabitant of temperate salt marshes. Molecular phylogenetic analysis of 16S rRNA gene clone libraries revealed four ribotypes associated with N. vectensis at multiple locations and times. These associates include two novel ribotypes within the ε-Proteobacterial order Campylobacterales and the Spirochetes, respectively, each sharing 99% 16S rRNA identity with Endozoicomonas elysicola and Pseudomonas oleovorans, respectively. Species-specific PCR revealed that these populations persisted in N. vectensis asecually propagated under laboratory …

Effect Of Harvest Method On In Vitro Digestibility Of Corn Residues, Janessa J. Updike, Jana L. Harding, Terry J. Klopfenstein Klopfenstein, James C. Macdonald

Nebraska Beef Cattle Reports

New corn residue harvesting methods were evaluated to determine the impacts of altering the proportions of plant part that are composed in a round bale. In vitro techniques were used to assess the organic matter digestibility of corn residue bale harvested with different proportions of stalks, leaves, and husk. As husk comprised a greater proportion of the bale, digestibility appeared to increase when compared with a conventional bale of cornstalks.

Exogenous Administration Of Lipids To Steers Alters Aspects Of The Innate Immune Response To Endotoxin Challenge, Nicole C. Burdick Sanchez, Jeffery A. Carroll, Janet R. Donaldson, Joe O. Buntyn, Ty B. Schmidt

Department of Animal Science: Faculty Publications

This study examined the effects of increasing energy availability from both dextrose and lipid treatments on the proinflammatory response to LPS in Holstein steers. Steers were randomly assigned to one of three groups: saline at 0.5 ml/ kg body weight (Control) or 50% dextrose [0.5 ml/kg body weight (Dextrose) to mimic calm cattle’s response to LPS] administered immediately prior to LPS (0.5 mg/kg body weight at 0 h) or continuous lipid emulsion infusion from –1 to 6 h [Intralipid 20% (Baxter, Deerfield, IL USA); 0.5 ml/kg/hr (Lipid) to mimic temperamental cattle]. Concentrations of non-esterified fatty acids (NEFA) were greater in …

Mesh Ora Framework: R/Bioconductor Packages To Support Mesh Over-Representation Analysis, Koki Tsuyuzaki, Gota Morota, Manabu Ishii, Takeru Nakazato, Satoru Miyazaki, Itoshi Nikaido

Department of Animal Science: Faculty Publications

Background: In genome-wide studies, over-representation analysis (ORA) against a set of genes is an essential step for biological interpretation. Many gene annotation resources and software platforms for ORA have been proposed. Recently, Medical Subject Headings (MeSH) terms, which are annotations of PubMed documents, have been used for ORA. MeSH enables the extraction of broader meaning from the gene lists and is expected to become an exhaustive annotation resource for ORA. However, the existing MeSH ORA software platforms are still not sufficient for several reasons.

Results: In this work, we developed an original MeSH ORA framework composed of six types of …

A Cooperative Study On The Standardized Total-Tract Digestible Phosphorus Requirement Of Twenty-Kilogram Pigs, O. Adeola, M. J. Azain, S. D. Carter, T. D. Crenshaw, M. J. Estienne, B. J. Kerr, M. D. Lindemann, C. V. Maxwell, P. S. Miller, M. C. Shannon, E. Van Heugten

Department of Animal Science: Faculty Publications

A cooperative study comprising growth performance, bone mineralization, and nutrient balance experiments was conducted at 11 stations to determine the standardized total-tract digestible (STTD) P requirement of 20-kg pigs using broken-line regression analysis. Monocalcium phosphate and limestone were added to a corn–soybean meal–based diet at the expense of cornstarch to establish 6 concentrations of STTD P from 1.54 to 5.15 g/kg in increments of 0.62 g/kg at a constant Ca:total P of 1.52:1.0. Diets were fed to 936 pigs (average initial BW of 19 kg) in 240 pens for 20 replicate pens of barrows and 20 replicate pens of gilts …

Yield And Forage Value Of A Dual-Purpose Bmr-12 Sorghum Hybrid, Melinda K. Yerka, Andrea K. Pederson, J. J. Toy, Galen E. Erickson, Jeffrey F. Pedersen, Rob B. Mitchell

Department of Animal Science: Faculty Publications

Grain sorghum [Sorghum bicolor (L.) Moench] is an important crop for rainfed production systems with 2.7 million ha grown in the United States in 2013. The brown-midrib (bmr) mutations, especially bmr-12, have resulted in low stover lignin and high fiber digestibility without reducing grain yield in some sorghum lines. However, the effect of the bmr trait on beef cattle (Bos taurus) performance when grazing crop residue is unknown. Our objectives were to validate previous small-plot results reporting no grain yield difference between near-isogenic bmr-12 (BMR) and wild-type control (CON) A Wheatland × R

Tx430 …

Cattle With Increased Severity Of Bovine Respiratory Disease Complex Exhibit Decreased Capacity To Protect Against Histone Cytotoxicity, J. A. Matera, B. K. Krehbiel, J. A. Hernandez Gifford, D. L. Step, C. R. Krehbiel, C. A. Gifford

Department of Animal Science: Faculty Publications

Bovine respiratory disease complex (BRDC) is the leading cause of morbidity and mortality in feedlot cattle. Significant inflammation and lesions are often observed in lungs of infected cattle. During acute inflammatory responses, histones contribute to mortality in rodents and humans and serum proteins can protect against histone-induced cytotoxicity. We hypothesized that cattle experiencing chronic or fatal cases of BRDC have reduced ability to protect against cytotoxic effects of histones. Serum samples were collected from 66 bull calves at the time of normal feedlot processing procedures. Animals were retrospectively assigned to groups consisting of calves never treated for BRDC (control [CONT]; …

The Effects Of Technology Use In Feedlot Production Systems On Feedlot Performance And Carcass Characteristics, C. L. Maxwell, B. C. Wilson, C. F. O'Neill, B. K. Wilson, C. G. Hixon, C. L. Haviland, A. N. Grimes, M. S. Calvo-Lorenzo, D. L. Vanoverbeke, G. G. Mafi, C. J. Richards, D. L. Step, B. P. Holland, C. R. Krehbiel

Department of Animal Science: Faculty Publications

The objectives of this study were to examine the effects of feedlot production systems with and without the use of a β-adrenergic agonist compared to an all-natural production program on feedlot performance and carcass characteristics. Crossbred beef steers (n = 336; initial BW = 379 ± 8 kg) were randomized to 1 of 3 treatments in a randomized complete block design (RCBD; 14 steers/pen; 8 pens/treatment). Treatments consisted of an all-natural treatment (NAT), a conventional treatment (CONV), and a conventional treatment with a β-agonist (CONV-Z). All treatments were fed the same basal diet with NAT cattle receiving no growth promoting …

Effect Of Lysozyme Or Antibiotics On Faecal Zoonotic Pathogens In Nursery Pigs, J E. Wells, E. D. Berry, N Kalchayanand, L A. Rampel, M Kim, W. T. Oliver

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aims: The objective of this study was to determine the effect of lysozyme and antibiotics on zoonotic pathogen shedding in faeces from nursery pigs housed without and with an indirect disease challenge. Methods and Results: Two replicates of approximately 650 pigs each were weaned and randomly assigned to one of 24 pens in either a nursery room that had been fully disinfected or a nursery room left unclean. Pigs were randomly assigned to control diet (Control), control diet + antibiotics (Antibiotic; chlortetracycline and tiamulin), or control diet + lysozyme (Lysozyme; 100 mg kg ^-1 diet). Rectal swab samples were collected …

Effects Of Atp And Atp Inhibitors On The P2x7 Receptor, Holly Marie Salzbrenner

Honors Program Theses

Extracellular ATP has been shown to increase the permeability of cells via the P2X7 receptor. This relationship could be important in understanding the physiology of the inflammation that occurs in bovine respiratory disease complex (BRDC). My research involved testing to see if this relationship between ATP and the P2X7 receptor is present. Then, potential inhibitors of ATP were tested so they can be used in further research involving ATP and BRDC. To test bovine cells, Mac-T cells were treated with ATP and incubated for various periods of time before Yo-Pro (a fluorescent molecule) was added. The percent change in fluorescence …

New Approaches To Corn Silage Use In Beef Cattle Finishing Diets, Dirk B. Burken

Department of Animal Science: Dissertations, Theses, and Student Research

Cattle feeders are considering alternative feedstuffs to replace expensive corn grain and decrease rations costs. Feeding corn silage allows cattle feeders to take advantage of the entire corn plant at a time of maximum quality and tonnage as well as secure substantial quantities of roughage and grain inventory. Distiller’s grains have proven to be economical and are now a commonplace ingredient in feedlot finishing diets. Experiments were conducted to determine the effects of feeding increased concentrations of corn silage in replacement of corn grain in finishing diets containing distillers grains. Feedlot gains and gain:feed ratios were reduced as corn silage …

Effects Of Feeding Distillers Grains Throughout A Yearling Heifer Beef Production System On Meat Quality Attributes And Effects Of Omnigen-Af With Ractopamine Hydrochloride On Animal Performance And Beef Carcass Characteristics Of Feedlot Steers, Kelby M. Sudbeck

Department of Animal Science: Dissertations, Theses, and Student Research

Heifers (n = 229; 225 kg ± 2) were used in a 2 x 2 x 2 factorial to determine the effects of longterm exposure to corn distillers grains plus solubles (DGS) on meat quality characteristics. Factors included supplementing 0.91 kg (LW) or 2.3 kg (HW) modified DGS (MDGS) while grazing corn stalks; 0.6% BW dry DGS (SS) daily or none (NS) during summer grazing; and finishing diet containing 40% wet corn gluten feed (CGF) or 40% MDGS (DGF) on a DM basis. An interaction was observed within CGF for discoloration, with SS increasing discoloration 30%; but no differences between …