Meat Science Commons^™

Formation And Characterization Of Lipid Droplets Of The Bovine Corpus Luteum, Heather A. Talbott, Michele R. Plewes, Crystal Krause, Xiaoying Hou, Pan Zhang, William B. Rizzo, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Establishment and maintenance of pregnancy depends on progesterone synthesized by luteal tissue in the ovary. Our objective was to identify the characteristics of lipid droplets (LDs) in ovarian steroidogenic cells. We hypothesized that LDs are a major feature of steroidogenic luteal cells and store cholesteryl esters. Whole bovine tissues, isolated ovarian steroidogenic cells (granulosa, theca, small luteal, and large luteal), and isolated luteal LDs were assessed for LD content, LD-associated proteins and lipid analyses. Bovine luteal tissue contained abundant lipid droplets, LD-associated perilipins 2/3/5, hormone-sensitive lipase, and 1-acylglycerol-3-phosphate O-acyltransferase ABHD5. Luteal tissue was enriched in triglycerides (TGs) compared to other …

Asas-Nanp Symposium: Ruminant/ Nonruminant Feed Composition: Challenges And Opportunities Associated With Creating Large Feed Composition Tables, Andres Schlageter-Tello, George C. Fahey, Tarra Freel, Liz Koutsos, Phillip S. Miller, William P. Weiss

Department of Animal Science: Faculty Publications

Traditional feed composition tables have been a useful tool in the field of animal nutrition throughout the last 70 yr. The objective of this paper is to discuss the challenges and opportunities associated with creating large feed ingredient composition tables. This manuscript will focus on three topics discussed during the National Animal Nutrition Program (NANP) Symposium in ruminant and nonruminant nutrition carried out at the American Society of Animal Science Annual Meeting in Austin, TX, on July 11, 2019, namely: 1) Using large datasets in feed composition tables and the importance of standard deviation in nutrient composition as well as …

Impact Of Cow Milk Production On Cow–Calf Performance In The Nebraska Sandhills, Tasha M. King, Jacki A. Musgrave, Richard N. Funston, J. Travis Mulliniks

Department of Animal Science: Faculty Publications

Livestock producers have tended to select for increased output traits like milk production and growth to increase productivity. Even with the increased selection for greater calf growth potential, some regions in the United States have seen a plateau in calf body weight (BW) at weaning (Lalman et al., 2019). When focusing on reaching maximum potential of these output traits, it is important to consider the multitude of variables that affect a production system. With increased milk production, nutrient requirements for cows become increased (Ferrell and Jenkins, 1984; Montaño-Bermudez et al., 1990), which may not be met if range and forage …

Influence Of Host Genetics In Shaping The Rumen Bacterial Community In Beef Cattle, Waseem Abbas, Jeremy T. Howard, Henry Paz, Kristin E. Hales, James E. Wells, Larry A. Kuehn, Galen E. Erickson, Matthew L. Spangler, Samodha Fernando

Department of Animal Science: Faculty Publications

In light of recent host-microbial association studies, a consensus is evolving that species composition of the gastrointestinal microbiota is a polygenic trait governed by interactions between host genetic factors and the environment. Here, we investigated the effect of host genetic factors in shaping the bacterial species composition in the rumen by performing a genome-wide association study. Using a common set of 61,974 single-nucleotide polymorphisms found in cattle genomes (n = 586) and corresponding rumen bacterial community composition, we identified operational taxonomic units (OTUs), Families and Phyla with high heritability. The top associations (1-Mb windows) were located on 7 chromosomes. These …

Genetic Structure And Admixture In Sheep From Terminal Breeds In The United States, Kimberly M. Davenport, C. Hiemke, Stephanie D. Mc Kay, J. W. Thorne, Ronald M. Lewis, Todd Taylor, B. M. Murdoch

Department of Animal Science: Faculty Publications

Selection for performance in diverse production settings has resulted in variation across sheep breeds worldwide. Although sheep are an important species to the United States, the current genetic relationship among many terminal sire breeds is not well characterized. Suffolk, Hampshire, Shropshire and Oxford (terminal) and Rambouillet (dual purpose) sheep (n = 248) sampled from different flocks were genotyped using the Applied Biosystems Axiom Ovine Genotyping Array (50K), and additional Shropshire sheep (n = 26) using the Illumina Ovine SNP50 BeadChip. Relationships were investigated by calculating observed heterozygosity, inbreeding coefficients, eigenvalues, pairwise Wright’s FST estimates and an identity by state matrix. …

Lamb Wool Shedding Is A Good Predictor Of Ewe Wool Shedding, Napoleón Vargas Jurado, Larry Kuehn, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Interest in reducing labour costs due to shearing has led to development of breed types that shed their wool naturally. Selection at young ages can facilitate response. Reliability of predictions of adult from lamb wool shedding (WS) is thus key in the design of breeding programmes to increase shedding. Our objectives were to estimate heritabilities and genetic relationships between WS measured once in lambs and repeatedly in ewes and to assess the accuracy of lamb WS EBV to predict ewe WS EBV based on a multi‐trait threshold or a repeatability model. Data were 4,971 lamb and 3,335 ewe WS records …

Effect Of Ammoniation And Harvest Method On Waste And Consumption Of Corn Residue Bales Fed To Cows In A Round Bale Feeder, Ashley C. Conway, Zachary E. Carlson, Fred H. Hilscher Jr, J. C. Macdonald, Terry J. Klopfenstein, Mary E. Drewnoski

Department of Animal Science: Faculty Publications

To determine the effects of harvest method and ammoniation (3.7% of dry matter) on consumption and waste of baled corn residue, a 6 × 6 Latin square with a 3 × 2 factorial treatment structure was conducted. Six treatments consisted of either nonammoniated or ammoniated residue, harvested one of three ways: conventional rake and bale (CONV), New Holland Cornrower with two rows of stem chopped into the windrow with tailings (2ROW), or EZBale system (EZB) with a disengaged combine spreader and tailings dropped in a windrow. Open cows were grouped by body weight to produce a light block of two …

The Effects Of 2 Genetic Lines On Spatial Distribution And Use And Preference Of Perch And Nest Area In An Aviary System, Sheila E. Scheideler, Pamela E. Eusebio Balcazar, K. Hanford

Department of Animal Science: Faculty Publications

The objective of this trial was to evaluate spatial distribution, nest and perch usage, and preference of Lohmann Brown (BH) and Bovan White (WH) Leghorn hens raised in an aviary system. At 5 wk, 400 floor raised pullets, BH and WH strains, in equal numbers, were placed into 8 modified Big Dutchman Natura aviary units. Each aviary unit had 3 tiers with perches and an indoor litter area. At 25 wk, the number of birds was recorded by scan sampling every 4 h. The number of birds perching in each aviary unit was recorded every 4 h at 15, 25, …

Beef Cows With Atypical Estrous Cyclicity At Puberty Produced Calves With Deficits In Preweaning Muscling, Metabolic Indicators, And Myoblast Function But Not In Feedlot Performance, Rebecca M. Swanson, Rachel L. Gibbs, Caitlin Cadaret, Galen E. Erickson, Ty B. Schmidt, Andrea Cupp, Dustin T. Yates

Department of Animal Science: Faculty Publications

In cattle, age at puberty and number of estrous cycles prior to first breeding contribute to lifetime reproductive success (Perry et al., 1991). In our university beef herd, we have identified a subset of cows that exhibited irregular pubertal cyclicity patterns between weaning and their first breeding season, which we postulate is associated with high androstenedione in follicular fluid (Cupp et al., 2019). Cows with high androstenedione are subfertile but wean calves that average 17 kg heavier than the herd average (Summers et al., 2014). We hypothesized that this additional weight at weaning in their offspring is due to superior …

Deficits In Growth, Muscle Mass, And Body Composition Following Placental Insufficiency-Induced Intrauterine Growth Restriction Persisted In Lambs At 60 D Of Age But Were Improved By Daily Clenbuterol Supplementation, Rachel L. Gibbs, Rebecca M. Swanson, Joslyn K. Beard, Ty B. Schmidt, Jessica Lynn Petersen, Dustin T. Yates

Department of Animal Science: Faculty Publications

Low birthweight in livestock results from stress-induced intrauterine growth restriction (IUGR; Yates et al., 2018). IUGR fetuses exhibit diminished muscle growth that persists in the neonatal stage, leading to asymmetric body composition and decreased weight gain (Cadaret et al., 2019). Ultimately, low birthweight diminishes yield and carcass merit at harvest (Greenwood et al., 2000), making effective postnatal treatment strategies to improve IUGR growth outcomes necessary. In this study, we examined the benefits of injecting the β2 agonist clenbuterol daily to target adrenergic adaptations that we previously observed in IUGR muscle (Posont et al., 2018; Yates et al., 2018). We hypothesized …

Beta-Adrenergic Agonists Increase Maximal Output Of Oxidative Phosphorylation In Bovine Satellite Cells, Renae L. Sieck, Leah K. Treffer, Martonio Ponte Viana, Oleh Khalimonchuk, Ty B. Schmidt, Dustin T. Yates, Jessica L. Petersen

Department of Animal Science: Faculty Publications

Beta-adrenergic agonists (βAA), Ractopamine HCl (RH) and Zilpaterol HCl (ZH), are FDAapproved supplements utilized in pigs and cattle to improve growth performance, carcass weight, and longissimus muscle area (Arp et al., 2014; Lean et al., 2014). Previous studies within our group have focused on understanding molecular changes in skeletal muscle due to βAA supplementation. This work has shown that βAA supplementation increases glucose oxidation in muscle from thermoneutral and heat-stressed lambs (Barnes et al., 2019) and in rat skeletal muscle stimulated with ZH (Cadaret et al., 2017). Skeletal muscle transcriptomics of lambs supplemented ZH revealed upregulation of genes associated with …

Heat Stress And Β-Adrenergic Agonists Alter The Adipose Transcriptome And Fatty Acid Mobilization In Ruminant Livestock, Rachel R. Reith, Renae L. Sieck, Pablo C. Grijalva, Erin M. Duffy, Rebecca M. Swanson, Anna M. Fuller, Kristin A. Beede, Joslyn K. Beard, Duarte E. Diaz, Ty B. Schmidt, Dustin T. Yates, Jessica L. Petersen

Department of Animal Science: Faculty Publications

Growth and feed efficiency of cattle are improved by supplementation with the beta-adrenergic agonists (βAA), ractopamine hydrochloride (RH; β₁AA) or zilpaterol hydrochloride (ZH; β₂AA) (Elam et al., 2009). βAA supplementation alters adipose deposition by inhibiting fatty acid biosynthesis and promoting lipolysis of stored triacylglycerols into free fatty acids (FFAs) (Johnson et al., 2014). However, β₂ adrenoceptors (βAR) desensitize with chronic activation (Re et al., 1997); supplementation is thus limited to the last 20 to 40 d of feeding.

The annual economic impact of heat stress (HS) has been estimated to exceed $2.4 billion (St-Pierre et …

Bovine Respiratory Disease Influences On Nutrition And Nutrient Metabolism, Clinton R. Krehbiel

Department of Animal Science: Faculty Publications

• Inflammation caused by bovine respiratory disease (BRD) continues to be one of the greatest challenges facing beef cattle producers and feedlot managers.

• BRD results in decreased intake, daily gain, and feed efficiency in feedlot calves, decreasing growth rate and increasing required days on feed.

• Morbidity caused by BRD has been associated with decreased hot carcass weight and poor carcass characteristics.

• Acute phase protein production in the liver, initiated by proinflammatory cytokines, may shift the priority for amino acid and energy use by the host animal during periods of sickness.

• Nutrient requirements for stressed calves seem …

Differential Longitudinal Establishment Of Human Fecal Bacterial Communities In Germ-Free Porcine And Murine Models, Nirosh Aluthge, Wesley A. Tom, Alison Bartenslager, Thomas E. Burkey, Phillip S. Miller, Kelly D. Heath, Craig Kreikemeier-Bower, Hatem Kittana, Robert Schmaltz, Amanda Ramer-Tait, Samodha Fernando

Department of Animal Science: Faculty Publications

The majority of microbiome studies focused on understanding mechanistic relationships between the host and the microbiota have used mice and other rodents as the model of choice. However, the domestic pig is a relevant model that is currently underutilized for human microbiome investigations. In this study, we performed a direct comparison of the engraftment of fecal bacterial communities from human donors between human microbiotaassociated (HMA) piglet and mouse models under identical dietary conditions. Analysis of 16S rRNA genes using amplicon sequence variants (ASVs) revealed that with the exception of early microbiota from infants, the more mature microbiotas tested established better …

Age Related Extracellular Matrix And Interstitial Cell Phenotype In Pulmonary Valves, Shaohua Wu, Vikas Kumar, Peng Xiao, Mitchell Kuss, Jung Yul Lim, Chittibabu Guda, Jonathan Butcher, Bin Duan

Department of Animal Science: Faculty Publications

Heart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 …

Susceptibility Of Swine Cells And Domestic Pigs To Sars-Cov-2, David A. Meekins, Igor Morozov, Jessie D. Trujillo, Natasha N. Gaudreault, Dashzeveg Bold, Mariano Carossino, Bianca L. Artiaga, Sabarish V. Indran, Taeyong Kwon, Velmurugan Balaraman, Daniel W. Madden, Heinz Feldmann, Jamie Henningson, Wenjun Ma, Udeni B. R. Balasuriya, Juergen A. Richt

Faculty Publications

No abstract provided.

Artificial Intelligence And Covid-19: Deep Learning Approaches For Diagnosis And Treatment, M. B. Jamshidi, A. Lalbakhsh, J. Talla, Z. Peroutka, F. Hadjilooei, P Lalbakhsh, M. Jamshidi, L. La Spada, M. Mirmozafari, M. Dehghani, A. Sabet, Sa. Roshani, So. Roshani, N. Bayat-Makou, B. Mohamadzade, Z. Malek, A. Jamshidi, S. Kiani, H. Hashemi-Dezaki, W. Mohyuddin

Faculty Publications

COVID-19 outbreak has put the whole world in an unprecedented difficult situation bringing life around the world to a frightening halt and claiming thousands of lives. Due to COVID-19 & x2019;s spread in 212 countries and territories and increasing numbers of infected cases and death tolls mounting to 5,212,172 and 334,915 (as of May 22 2020), it remains a real threat to the public health system. This paper renders a response to combat the virus through Artificial Intelligence (AI). Some Deep Learning (DL) methods have been illustrated to reach this goal, including Generative Adversarial Networks (GANs), Extreme Learning Machine (ELM), …

The “Challenge" Of Depletion: Why The Oyster Fishery Is Not Self-Regulating, Eric N. Powell, John M. Klinck, Leanne M. Poussard

CCPO Publications

The possibility that the economics of the oyster fishery impose a self-limitation on overharvesting has been proffered on occasion. The inefficiency of harvesting by the fishery has been evaluated and estimates of the exploitation rate permissible under conditions of maximum sustainable yield have been obtained in previous studies. The question becomes to what extent does the inefficiency of harvest interact with the economics of the fishery to compromise ready detection of overfishing? This study explores the possibility that the constraint of economics on the fishery occurs at oyster exploitation rates that are higher than maximum sustainable yield, leading ineluctably to …

Innovation In Meat Production: A Problem And An Opportunity, Christopher J. Bryant

Animal Sentience

Innovation in meat production has enabled modern humans to inflict far greater harm on animals, the environment, and public health than was possible just a few decades ago. Wiebers & Feigin aptly express the urgency with which these issues must be addressed. Those advocating for animals on moral grounds face resistance from omnivores citing taste, price and convenience. Further innovation in meat production (plant-based and cultured meat) will enable us to preserve the experience of eating meat whilst phasing out the many problems caused by industrial animal farming.

Effect Of Different Fat Sources And Vitamin E Isoforms/ Levels On Carcass Characteristics, Meat Quality, And Belly/Bacon Characteristics Of Pigs Grown To Heavy Slaughter Weights (>150kg), Marlee Kelley

Theses and Dissertations--Animal and Food Sciences

Two separate studies were conducted to evaluate the potential interaction of fat sources and vitamin E (VE) on heavy slaughter weights. In Study 1, a total of 64 individually-fed pigs (28.41 ± 0.83 kg) were randomly assigned to 8 dietary treatments in a 4×2 factorial arrangement. Fat treatments included cornstarch (CS), tallow (TW), corn oil (CO), and coconut-oil (CN). VE treatments were dietary alpha-tocopheryl-acetate (ATA) at 11 and 200 ppm. In Study 2, a total of 72 individually fed pigs (28.55 ± 1.16 kg) were randomly assigned to 12 dietary treatments in a 2 × 6 factorial arrangement. Fat treatments …