Dairy Science Commons^™

Investigation Of Methionine And Lysine Derivatives As A Source Of Rumen-Protected Amino Acids For Lactating Dairy Cows, Mark Avila Fagundes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cows have a protein requirement for growth, maintenance, and lactation. In order to meet those protein requirements, dairy farmers can supplement or feed cattle with specific amino acids, the building blocks that make-up protein. However, in order for the amino acid product to be effective it must avoid degradation in the rumen and be delivered in the small intestine for absorption. Lysine and methionine have traditionally been recognized as the most limiting amino acids for lactating dairy cows. Therefore, nutrition companies have focused on finding ways to encapsulate or protect lysine and methionine from rumen microbes. The N-acetyl-L-methionine and N-acetyl-L-lysine …

The Effects Of Trace Mineral Supplementation On Performance, Health, And Carcass Quality Of At-Risk Mineral Deficient Feedlot Cattle, Tevan J. Brady

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Morbidity in feedlot cattle due to disease is a common economical loss for feedlot producers. Utah is not typically considered a ‘feedlot state’, but there are several producers in the southern part of the state that specialize in receiving at-risk cattle. These cattle are at-risk because they are coming from areas known to be mineral deficient. Areas such as the western US, are known to be deficient in several trace minerals important in immune response. Therefore, it is critical that producers have an adequate plan to decrease the negative effects that this has on economic viability of their beef operations, …

Detecting Dna Oxidation In Sperm, Alexie Zwerdling

Student Research Symposium

Declining body-wide levels of nicotinamide adenine dinucleotide (NAD) that are present in normal aging men are linked to decreased sperm quality and DNA integrity. When NAD is low, the amount of oxidative stress on the DNA appears to be increased, due to the limited regeneration of the antioxidant glutathione, which is NAD-dependent. We therefore hypothesize that the increased sperm DNA damage in the aging male is caused by an elevated formation of 8-oxoguanine in the DNA, caused by increased oxidative stress when NAD levels are low. To test this hypothesis, a transgenic mouse model (ANDY mouse) was used to create …

Using Handmade Cloning To Reduce Oocyte Mitochondria In Reconstructed Embryos, Laura Adams

Student Research Symposium

Mitochondrial heteroplasmy, the presence of more than one population of mitochondrial DNA (mtDNA) in one cell, can cause metabolic issues and reduced ATP synthesis, even at relatively low levels. The presence of this condition in somatic cell nuclear transfer (SCNT) embryos likely contributes to reduced prenatal and postnatal viability: the majority of the oocyte’s mitochondria is still present at the time of nuclear transfer, as well as through any further embryonic development post-fusion and activation. Though the somatic cell also contributes its mitochondria (which can successfully communicate with its nucleus), the oocyte’s mitochondria compose a greater proportion of the total …

Roles Of Nad+ In Testicular Aging And Epigenetic Sperm Modifications, Renata Hoskova

Student Research Symposium

Since 1980, the average age of first-time fathers has been increasing. With increased age, sperm chromatin quality and nicotinamide adenine dinucleotide (NAD) levels are reduced. In order to condense around protamines, sperm DNA undergoes breaks, at which time epigenetic reprogramming occurs. In humans, about 2% of DNA stays wound to histones in spermatozoa. Histones carry post-translational modifications that control gene activity and have the potential to transmit epigenetic information to the offspring. Analysis of epigenetic sperm modifications is being conducted within niacin deficient versus niacin replete mice. The experimental plan includes the characterization of histone exchange in elongating spermatids via …

Bolstering Southeast Asia's Livestock Biosecurity And Climate Change Resilience Essential For Global Health, Madelyn Kunzler

Student Research Symposium

A large factor of climate change is its effect on infectious zoonotic diseases. Zoonotic diseases are diseases that can be transferred from animals into humans. Cases of zoonotic diseases have increased as humans have interacted more with animals, and as worldwide travel has become more frequent. It is predicted that 60% of emerging human diseases will be zoonotic. The cases will continue to increase to due climate change. As climate change continues, research has shown that new diseases will emerge from the melting permafrost and ice caps. It is also likely that pre-existing diseases will be able to migrate to …

Lymphocyte Soluble Factors From Pregnant Cows Modulate Mrna Transcript Abundances Encoding For Proteins Associated With Trophoblast Growth And Development, Kelsy A. Leppo, Preston Collins, Kira Morgado, Ana C. Silva, Aaron Thomas, Heloisa M. Rutigliano

Animal, Dairy, and Veterinary Science Faculty Publications

This study was conducted to determine whether T cell populations are responsible for modulating placental development during gestation in cattle. It was hypothesized that CD4+CD25+ and γ/δ+ T cells modulate gene expression, based on mRNA transcript abundances, and promote proliferation and survival of trophoblast cells. Peripheral blood was collected from cows at 160 to 180 days of gestation and non-pregnant cows, T cell populations CD8+, CD4+, CD4+CD25+, CD24+CD25-, and γ/δ+ T cells were isolated, cultured for 48 h, and supernatant was collected. Placental samples were digested, and trophoblast cells were cultured for 24 h. Trophoblast cells were cultured with 50 …

Managing And Buying Bulls, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Weaning percentage and therefore conception rate, can significantly influence profitability in the breeder herd.

Bulls need to be managed to optimise their performance for the mating period to maximise conception rates. For producers retaining females in the breeding herd, bulls can have a significant impact on future herd productivity through the genetics they pass on.

Various tools are available to assess how a bull will perform before purchase, including visual appraisal, obtaining a bull breeding soundness examination (BBSE) and utilising estimated breeding values (EBVs).

Mice Pups Breastfed By Foster Mothers Whose Breast Milk Contains Plasmodium Falciparum Recombinant Se36 Antigen Develop Specific Antibodies, Margaret Mendi Njoroge, Victor Irungu Mwangi, Tonny Jimmy Owalla, Hastings Ozwara, Thomas G. Egwang

All Peer-Reviewed Publications

Intranasal instillation of SE36, a malaria vaccine candidate antigen, in lactating BALB/c strain (derived from the Bagg and albino laboratory inbred mice) female mice resulted in the appearance of the antigen in breast milk as demonstrated by sandwich ELISA and Western blot. Pups born of immunologically naive mice and breastfed on lactating foster mothers exposed intranasally to SE36 developed IgG anti-SE36 antibodies. These data demonstrate that maternal immunization in mice by this route in lactating mothers can result in active immunization of offspring via ingestion of breast milk containing antigen. If confirmed in a nonhuman primate model and in human …

Jackrabbit Dairy & Food Review, Winter 2020-2021, Dairy And Food Science Department

The Jackrabbit Dairy & Food Review

This is the Winter 2020- 2021 Jackrabbit Dairy and Food Review.

It contains the following articles and information:
Message from the Department Head
New Personnel
Judging Activities
Dairy Club
Food Science Club
Student Accomplishments
Professional Activities of Faculty and Staff
Dairy Facilities
2020 Graduates
Where Are They Now?
Scholarships
Dairy and Food Science Students
Outreach
Research Highlights

Dairy Digest 2021 : Striving To Support And Sustain, South Dakota State University Dairy Club

Dairy Digest

This is the 2021 Dairy Digest published annually by the South Dakota State University Dairy Club. The Digest contains information and news about the activities and members of the SDSU Dairy Club for the academic year 2020 - 2021.

Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez

Electronic Theses and Dissertations

This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …

Milk Phospholipids Extraction Using Switchable Solvents, Kaavya Rathnakumar

Electronic Theses and Dissertations

Phospholipids (PLs) found in milk are of great interest due to their health and nutritional benefits associated with their consumption. By-products generated during the manufacture of dairy products represent a source of PLs with potential for value-added opportunities. Currently, the extraction of PLs from by-products results in overall lowefficiencies, and it involves subsequent solvent separation and lipid recovery. This dissertation studied the extraction of milk PLs from by-product streams with a tertiary anime (N, N-dimethyl cyclohexylamine, CyNMe2) as a switchable hydrophilicity solvent. This type of solvents can be reversibly switched between a hydrophobic and hydrophilic form in the presence or …

Gene Network And Pathway Analysis Of Transcriptional Signatures Characterizing Sole Ulcer And Digital Dermatitis In Dairy Cows, Roshin Anie Mohan

Electronic Theses and Dissertations

This study aimed to evaluate the transcriptomic profile of both corium tissue from lactating dairy cows experiencing sole ulcers and skin lesions from lactating dairy cows diagnosed with digital dermatitis (DD). Hoof biopsies were performed in multiparous Holstein dairy cows selected based on their condition as clinically healthy or diagnosed with sole ulcers (n=7/group). Similarly, skin biopsies were taken from the center of active (M2/M4.1) DD lesions or non-active (M0/M4/M1) from multiparous Holstein dairy cows (n = 7/group) to assess the impact of DD on the skin transcriptome via RNA-seq analysis. All RNA samples were sequenced using Illumina, NovaSeq S4 …

Developing Whey Protein Encapsulated Probiotics And Evaluating Environmental Listeria Using Whole-Genome Sequencing, Shayanti Minj

Electronic Theses and Dissertations

In this study, a novel value-added dairy-based health formulation was developed using whey protein hydrolysate and probiotic organisms. In the first part of the study, different forms of whey proteins, concentrate WPC80, isolate WPI90, and hydrolysates WPH10, WPH15 and WPH20 were screened for bioactivities (antimicrobial, antioxidant, and antihypertensive activity). Hydrolysate WPH10, exhibiting the highest bioactivities was conjugated with maltodextrin. A batch of 2L conjugated solution was spray dried in a Niro drier with an inlet and outlet temperature of 200⁰C and 90⁰C, and alternatively, freeze dried at −80 °C under 50 mTorr vacuum. The bioactivities of the conjugated samples were …

An Exploration Of The Effects Of An Early Postpartum Intravenous Infusion With Carnosic Acid On Physiological Responses Of Transition Dairy Cows, Tainara Michelotti

Electronic Theses and Dissertations

The transition period is commonly defined as the last 3 wk before parturition to 3 wk after parturition. It is the most critical stage of the lactation cycle of high-producing dairy cows. The period consists of a complex interplay of metabolic and hormonal adaptations, inflammation, and immune activation. During the transition period, dairy cows commonly face oxidative stress, an underlying factor for dysfunctional immune response and enhanced inflammation, which can further increase the susceptibility of dairy cattle to health disorders and decrease productivity. In the last few decades, there has been an increased interest in studying alternative natural compounds with …

Cattle Industry Funding Scheme Annual Report 2019/2020, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

In June 2010 the Cattle Industry Funding Scheme (IFS) was introduced to address biosecurity threats relevant to the Western Australian (WA) cattle industry. The Scheme was established under the Biosecurity and Agriculture Management Act 2007 to enable cattle producers to identify the pest and disease priorities at a whole-of-industry level and to raise funds for activities to address these priorities.

There are currently three Industry Funding Schemes in operation. In addition to the Cattle IFS, there is a Sheep and Goat IFS and a Grains, Seeds and Hay IFS. The three IFSs operate in a similar manner.

The purpose of …

Isolation Box Test (Ibt) And Dairy Calf Personality Traits Relationship With Performance Through Weaning, Megan Marie Woodrum Setser

Theses and Dissertations--Animal and Food Sciences

Personality tests are utilized to characterize individual differences in personality traits across species. Currently, there is no objective personality test that can be implemented on-farm to measure personality in dairy calves. Measuring personality of youngstock in the dairy industry may allow producers to utilize information on individual differences for management decisions and limit the investment in unfit individuals. The isolation box test is a personality test that has potential to be adapted and utilized for dairy calves. The main objective of this thesis is to develop, validate, and utilize an isolation box test for use on-farm in dairy calves. More …

Single-Dose Oral Challenges To Validate Eliciting Doses In Children With Cow’S Milk Allergy, Paul J. Turner, Yvonne M. D'Art, Bettina Duca, Sophia A. Chastell, Guadalupe Marco-Martin, Rosialzira N. Vera-Berrios, Olaya Alvarez, Raphaëlle Bazire, Pablo Rodríguez Del Río, Marta Vazquez-Ortiz, Joseph L. Baumert, Ronald Van Ree, Clare E.N. Mills, Montserrat Fernandez-Rivas, Jonathan O.B. Hourihane

Department of Food Science and Technology: Faculty Publications

Background: There is increasing interest in the use of eliciting doses (EDs) to inform allergen risk management. The ED can be estimated from the distribution of threshold doses for allergic subjects undergoing food challenges within a specified population. Estimated ED₀₅ values for cow's milk (the dose expected to cause objective allergic symptoms in 5% of the milk-allergic population) range from 0.5 mg to 13.9 mg cow's milk protein. We undertook a single-dose challenge study to validate a predicted ED₀₅ for cow's milk of 0.5 mg protein.

Methods: Participants were recruited from 4 clinical centres. Predetermined criteria were used …

Incorporation Of Summer Annual Mixtures Into Grazing Systems In Kentucky, Kelly Marie Mercier

Theses and Dissertations--Plant and Soil Sciences

Utilizing summer annual grass-legume forage mixtures has the potential to improve forage yield and nutritive characteristics, and/or animal performance during times when cool-season pasture growth is limited by high temperatures. Legumes can utilize atmospheric nitrogen, which can increase crude protein and forage digestibility in mixtures. As nitrogen application generally improves both the yield and nutritive characteristics of summer annual forages, but can have a negative effect on legume competitiveness, nitrogen fertilizer recommendations for legume-containing summer annual mixtures are not well established.

Two experiments were conducted to determine the feasibility of utilizing summer annual mixtures in Kentucky, USA. The first experiment …